BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00027425
         (471 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PIN25004.1| Serine/threonine protein kinase [Handroanthus imp...   183   5e-53
gb|KZV27376.1| hypothetical protein F511_02485 [Dorcoceras hygro...   172   3e-49
ref|XP_011070074.1| SNF1-related protein kinase catalytic subuni...   164   1e-45
ref|XP_012828001.1| PREDICTED: SNF1-related protein kinase catal...   158   8e-45
gb|EYU18585.1| hypothetical protein MIMGU_mgv1a019318mg [Erythra...   158   6e-44
emb|CDP06261.1| unnamed protein product [Coffea canephora]            160   1e-43
ref|XP_017243775.1| PREDICTED: SNF1-related protein kinase catal...   155   6e-42
gb|PLY75569.1| hypothetical protein LSAT_9X32220 [Lactuca sativa]     147   1e-39
ref|XP_023731702.1| SNF1-related protein kinase catalytic subuni...   147   2e-39
ref|XP_021988779.1| SNF1-related protein kinase catalytic subuni...   146   1e-38
ref|XP_022872089.1| SNF1-related protein kinase catalytic subuni...   143   7e-38
ref|XP_022872088.1| SNF1-related protein kinase catalytic subuni...   143   7e-38
ref|XP_010653647.1| PREDICTED: SNF1-related protein kinase catal...   144   1e-37
ref|XP_022872087.1| SNF1-related protein kinase catalytic subuni...   143   1e-37
ref|XP_019077464.1| PREDICTED: SNF1-related protein kinase catal...   144   1e-37
ref|XP_022872086.1| SNF1-related protein kinase catalytic subuni...   143   2e-37
ref|XP_022872085.1| SNF1-related protein kinase catalytic subuni...   143   2e-37
ref|XP_022872084.1| SNF1-related protein kinase catalytic subuni...   143   2e-37
emb|CBI32832.3| unnamed protein product, partial [Vitis vinifera]     144   2e-37
gb|KVH94776.1| Kinase associated domain 1 (KA1) [Cynara carduncu...   143   3e-37

>gb|PIN25004.1| Serine/threonine protein kinase [Handroanthus impetiginosus]
          Length = 467

 Score =  183 bits (465), Expect = 5e-53
 Identities = 90/101 (89%), Positives = 97/101 (96%)
 Frame = -1

Query: 303 VLPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLF 124
           VLPRYK GKTLGIG+FGKVKLAVHTLTGLKVAIKIL+RQS+D+ EAEKVRREI+IMRQLF
Sbjct: 12  VLPRYKIGKTLGIGAFGKVKLAVHTLTGLKVAIKILNRQSMDNAEAEKVRREINIMRQLF 71

Query: 123 HPHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           HPH+VRLFEVIDTPS+VYVVMEYMNSGELFYYLTE GKL E
Sbjct: 72  HPHIVRLFEVIDTPSNVYVVMEYMNSGELFYYLTEKGKLRE 112


>gb|KZV27376.1| hypothetical protein F511_02485 [Dorcoceras hygrometricum]
          Length = 402

 Score =  172 bits (436), Expect = 3e-49
 Identities = 83/101 (82%), Positives = 95/101 (94%)
 Frame = -1

Query: 303 VLPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLF 124
           VLP YK G+TLGIG+FGKVKLAVHTLTGLKVAIKILDR+SID  EAEKVR+EI+IM+QLF
Sbjct: 16  VLPCYKLGRTLGIGAFGKVKLAVHTLTGLKVAIKILDRRSIDSSEAEKVRKEINIMKQLF 75

Query: 123 HPHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           HPH+VRLFEVIDTP+ VYVVMEYMNSGELFY++TE+G+L E
Sbjct: 76  HPHIVRLFEVIDTPTKVYVVMEYMNSGELFYFITEHGRLDE 116


>ref|XP_011070074.1| SNF1-related protein kinase catalytic subunit alpha KIN10-like
           isoform X1 [Sesamum indicum]
          Length = 470

 Score =  164 bits (415), Expect = 1e-45
 Identities = 82/101 (81%), Positives = 90/101 (89%)
 Frame = -1

Query: 303 VLPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLF 124
           +L RYK GKTLG G  GKVKLAVH LTGLKVAIKI D +SI + EAEKVRREI+IM+QLF
Sbjct: 15  LLLRYKVGKTLGFGLSGKVKLAVHNLTGLKVAIKIFDVRSIANVEAEKVRREINIMKQLF 74

Query: 123 HPHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           HPH+VRLFEVIDTPS+VYVVMEYM+SGELFYYLTE GKLHE
Sbjct: 75  HPHIVRLFEVIDTPSYVYVVMEYMSSGELFYYLTEKGKLHE 115


>ref|XP_012828001.1| PREDICTED: SNF1-related protein kinase catalytic subunit alpha
           KIN10-like [Erythranthe guttata]
          Length = 316

 Score =  158 bits (400), Expect = 8e-45
 Identities = 77/97 (79%), Positives = 90/97 (92%)
 Frame = -1

Query: 303 VLPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLF 124
           +L RYK  K LGIG+FGKVKLAVH+LTG+KVAIKILDR+SI++CEAEKVRREI+IMR+L 
Sbjct: 10  ILSRYKIEKRLGIGTFGKVKLAVHSLTGIKVAIKILDRRSINNCEAEKVRREINIMRRLS 69

Query: 123 HPHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENG 13
           HP+VVRLFEVI+TPS VYVVMEY+NSGELFYYL +NG
Sbjct: 70  HPNVVRLFEVIETPSKVYVVMEYLNSGELFYYLIDNG 106


>gb|EYU18585.1| hypothetical protein MIMGU_mgv1a019318mg [Erythranthe guttata]
          Length = 402

 Score =  158 bits (400), Expect = 6e-44
 Identities = 77/97 (79%), Positives = 90/97 (92%)
 Frame = -1

Query: 303 VLPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLF 124
           +L RYK  K LGIG+FGKVKLAVH+LTG+KVAIKILDR+SI++CEAEKVRREI+IMR+L 
Sbjct: 10  ILSRYKIEKRLGIGTFGKVKLAVHSLTGIKVAIKILDRRSINNCEAEKVRREINIMRRLS 69

Query: 123 HPHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENG 13
           HP+VVRLFEVI+TPS VYVVMEY+NSGELFYYL +NG
Sbjct: 70  HPNVVRLFEVIETPSKVYVVMEYLNSGELFYYLIDNG 106


>emb|CDP06261.1| unnamed protein product [Coffea canephora]
          Length = 536

 Score =  160 bits (405), Expect = 1e-43
 Identities = 75/101 (74%), Positives = 89/101 (88%)
 Frame = -1

Query: 303 VLPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLF 124
           VL  Y+ GKTLG+G F KVKLAVH LTG+KVAIKILDRQ++D+ E  KVRRE++IMRQL 
Sbjct: 52  VLSNYRLGKTLGVGCFAKVKLAVHNLTGIKVAIKILDRQTLDEFETMKVRREVNIMRQLS 111

Query: 123 HPHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           HPH+VRLFEVI+T SH+YVVMEYMNSGELFYY+TE G+L+E
Sbjct: 112 HPHIVRLFEVIETTSHIYVVMEYMNSGELFYYITEKGRLNE 152


>ref|XP_017243775.1| PREDICTED: SNF1-related protein kinase catalytic subunit alpha
           KIN10-like [Daucus carota subsp. sativus]
 gb|KZM99105.1| hypothetical protein DCAR_013533 [Daucus carota subsp. sativus]
          Length = 493

 Score =  155 bits (391), Expect = 6e-42
 Identities = 75/101 (74%), Positives = 91/101 (90%)
 Frame = -1

Query: 303 VLPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLF 124
           VLP YK GKTLGIG+FGKVKLA H LTG+KVA+KIL+RQSID+  AEKVRREI+I+R L 
Sbjct: 15  VLPNYKIGKTLGIGAFGKVKLAHHILTGIKVAVKILERQSIDEKAAEKVRREINILRLLS 74

Query: 123 HPHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           HPHVVRLFEVI+TP+ +Y+VMEYM+SGELF ++TENG+L+E
Sbjct: 75  HPHVVRLFEVIETPTTIYMVMEYMDSGELFDHITENGRLNE 115


>gb|PLY75569.1| hypothetical protein LSAT_9X32220 [Lactuca sativa]
          Length = 410

 Score =  147 bits (371), Expect = 1e-39
 Identities = 72/100 (72%), Positives = 86/100 (86%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           L  Y+ GKTLGIG+FG+VK+A H LTG+KVAIKIL RQSI+D  AEKVRREI+IMR L H
Sbjct: 15  LSNYRMGKTLGIGAFGRVKVAQHILTGVKVAIKILARQSINDSAAEKVRREINIMRLLSH 74

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRL+EVI+T S +YV+MEYMNSGELF Y+TENG+L E
Sbjct: 75  PHIVRLYEVIETRSCIYVIMEYMNSGELFDYITENGRLEE 114


>ref|XP_023731702.1| SNF1-related protein kinase catalytic subunit alpha KIN10-like
           [Lactuca sativa]
          Length = 441

 Score =  147 bits (371), Expect = 2e-39
 Identities = 72/100 (72%), Positives = 86/100 (86%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           L  Y+ GKTLGIG+FG+VK+A H LTG+KVAIKIL RQSI+D  AEKVRREI+IMR L H
Sbjct: 15  LSNYRMGKTLGIGAFGRVKVAQHILTGVKVAIKILARQSINDSAAEKVRREINIMRLLSH 74

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRL+EVI+T S +YV+MEYMNSGELF Y+TENG+L E
Sbjct: 75  PHIVRLYEVIETRSCIYVIMEYMNSGELFDYITENGRLEE 114


>ref|XP_021988779.1| SNF1-related protein kinase catalytic subunit alpha KIN11-like
           [Helianthus annuus]
 gb|OTG11417.1| putative serine/threonine-protein kinase, SIK1/2, KA1 domain/Ssp2
           [Helianthus annuus]
          Length = 492

 Score =  146 bits (369), Expect = 1e-38
 Identities = 71/100 (71%), Positives = 84/100 (84%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           L  Y+ GKTLG G+FGKVK+A H LTG+KVAIKILDRQSI+D + E+VRREI IMR L H
Sbjct: 13  LSNYRLGKTLGFGAFGKVKVAQHVLTGIKVAIKILDRQSINDSDGERVRREIKIMRLLSH 72

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRL+EVI+T S +YVVMEYMNSGELF Y+TEN +L E
Sbjct: 73  PHIVRLYEVIETQSTIYVVMEYMNSGELFDYITENRRLEE 112


>ref|XP_022872089.1| SNF1-related protein kinase catalytic subunit alpha KIN10-like
           isoform X6 [Olea europaea var. sylvestris]
          Length = 422

 Score =  143 bits (360), Expect = 7e-38
 Identities = 71/100 (71%), Positives = 87/100 (87%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           LP YKF +TLGIG+FGKVKLAVHTLTGL VAIKILDR+SI++ +A+KVRREI+IM++L H
Sbjct: 26  LPHYKFVRTLGIGAFGKVKLAVHTLTGLNVAIKILDRESINNLDADKVRREINIMKRLSH 85

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRLFEVIDT S VYVVME+M+ GEL+ Y+T   +L E
Sbjct: 86  PHIVRLFEVIDTQSKVYVVMEHMDLGELYDYVTLRVRLDE 125


>ref|XP_022872088.1| SNF1-related protein kinase catalytic subunit alpha KIN10-like
           isoform X5 [Olea europaea var. sylvestris]
          Length = 427

 Score =  143 bits (360), Expect = 7e-38
 Identities = 71/100 (71%), Positives = 87/100 (87%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           LP YKF +TLGIG+FGKVKLAVHTLTGL VAIKILDR+SI++ +A+KVRREI+IM++L H
Sbjct: 26  LPHYKFVRTLGIGAFGKVKLAVHTLTGLNVAIKILDRESINNLDADKVRREINIMKRLSH 85

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRLFEVIDT S VYVVME+M+ GEL+ Y+T   +L E
Sbjct: 86  PHIVRLFEVIDTQSKVYVVMEHMDLGELYDYVTLRVRLDE 125


>ref|XP_010653647.1| PREDICTED: SNF1-related protein kinase catalytic subunit alpha
           KIN10 isoform X2 [Vitis vinifera]
 ref|XP_010653648.1| PREDICTED: SNF1-related protein kinase catalytic subunit alpha
           KIN10 isoform X2 [Vitis vinifera]
 ref|XP_010653649.1| PREDICTED: SNF1-related protein kinase catalytic subunit alpha
           KIN10 isoform X2 [Vitis vinifera]
          Length = 494

 Score =  144 bits (362), Expect = 1e-37
 Identities = 69/100 (69%), Positives = 85/100 (85%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           L  Y+ GKTLGIG+FGKVK+A+HT T LKVAIKILDRQSIDD  A++V+REI+I+R   H
Sbjct: 14  LGNYRLGKTLGIGAFGKVKVALHTTTKLKVAIKILDRQSIDDSTADRVKREINILRLFSH 73

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRL+EVI+T + +YVVMEYMNSGELF Y+TEN +L E
Sbjct: 74  PHIVRLYEVIETRTKIYVVMEYMNSGELFDYITENDRLQE 113


>ref|XP_022872087.1| SNF1-related protein kinase catalytic subunit alpha KIN10-like
           isoform X4 [Olea europaea var. sylvestris]
          Length = 455

 Score =  143 bits (360), Expect = 1e-37
 Identities = 71/100 (71%), Positives = 87/100 (87%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           LP YKF +TLGIG+FGKVKLAVHTLTGL VAIKILDR+SI++ +A+KVRREI+IM++L H
Sbjct: 26  LPHYKFVRTLGIGAFGKVKLAVHTLTGLNVAIKILDRESINNLDADKVRREINIMKRLSH 85

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRLFEVIDT S VYVVME+M+ GEL+ Y+T   +L E
Sbjct: 86  PHIVRLFEVIDTQSKVYVVMEHMDLGELYDYVTLRVRLDE 125


>ref|XP_019077464.1| PREDICTED: SNF1-related protein kinase catalytic subunit alpha
           KIN10 isoform X1 [Vitis vinifera]
          Length = 514

 Score =  144 bits (362), Expect = 1e-37
 Identities = 69/100 (69%), Positives = 85/100 (85%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           L  Y+ GKTLGIG+FGKVK+A+HT T LKVAIKILDRQSIDD  A++V+REI+I+R   H
Sbjct: 34  LGNYRLGKTLGIGAFGKVKVALHTTTKLKVAIKILDRQSIDDSTADRVKREINILRLFSH 93

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRL+EVI+T + +YVVMEYMNSGELF Y+TEN +L E
Sbjct: 94  PHIVRLYEVIETRTKIYVVMEYMNSGELFDYITENDRLQE 133


>ref|XP_022872086.1| SNF1-related protein kinase catalytic subunit alpha KIN10-like
           isoform X3 [Olea europaea var. sylvestris]
 ref|XP_022872091.1| SNF1-related protein kinase catalytic subunit alpha KIN10-like
           isoform X3 [Olea europaea var. sylvestris]
          Length = 474

 Score =  143 bits (360), Expect = 2e-37
 Identities = 71/100 (71%), Positives = 87/100 (87%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           LP YKF +TLGIG+FGKVKLAVHTLTGL VAIKILDR+SI++ +A+KVRREI+IM++L H
Sbjct: 16  LPHYKFVRTLGIGAFGKVKLAVHTLTGLNVAIKILDRESINNLDADKVRREINIMKRLSH 75

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRLFEVIDT S VYVVME+M+ GEL+ Y+T   +L E
Sbjct: 76  PHIVRLFEVIDTQSKVYVVMEHMDLGELYDYVTLRVRLDE 115


>ref|XP_022872085.1| SNF1-related protein kinase catalytic subunit alpha KIN10-like
           isoform X2 [Olea europaea var. sylvestris]
          Length = 480

 Score =  143 bits (360), Expect = 2e-37
 Identities = 71/100 (71%), Positives = 87/100 (87%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           LP YKF +TLGIG+FGKVKLAVHTLTGL VAIKILDR+SI++ +A+KVRREI+IM++L H
Sbjct: 26  LPHYKFVRTLGIGAFGKVKLAVHTLTGLNVAIKILDRESINNLDADKVRREINIMKRLSH 85

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRLFEVIDT S VYVVME+M+ GEL+ Y+T   +L E
Sbjct: 86  PHIVRLFEVIDTQSKVYVVMEHMDLGELYDYVTLRVRLDE 125


>ref|XP_022872084.1| SNF1-related protein kinase catalytic subunit alpha KIN10-like
           isoform X1 [Olea europaea var. sylvestris]
          Length = 484

 Score =  143 bits (360), Expect = 2e-37
 Identities = 71/100 (71%), Positives = 87/100 (87%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           LP YKF +TLGIG+FGKVKLAVHTLTGL VAIKILDR+SI++ +A+KVRREI+IM++L H
Sbjct: 26  LPHYKFVRTLGIGAFGKVKLAVHTLTGLNVAIKILDRESINNLDADKVRREINIMKRLSH 85

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRLFEVIDT S VYVVME+M+ GEL+ Y+T   +L E
Sbjct: 86  PHIVRLFEVIDTQSKVYVVMEHMDLGELYDYVTLRVRLDE 125


>emb|CBI32832.3| unnamed protein product, partial [Vitis vinifera]
          Length = 557

 Score =  144 bits (362), Expect = 2e-37
 Identities = 69/100 (69%), Positives = 85/100 (85%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           L  Y+ GKTLGIG+FGKVK+A+HT T LKVAIKILDRQSIDD  A++V+REI+I+R   H
Sbjct: 77  LGNYRLGKTLGIGAFGKVKVALHTTTKLKVAIKILDRQSIDDSTADRVKREINILRLFSH 136

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRL+EVI+T + +YVVMEYMNSGELF Y+TEN +L E
Sbjct: 137 PHIVRLYEVIETRTKIYVVMEYMNSGELFDYITENDRLQE 176


>gb|KVH94776.1| Kinase associated domain 1 (KA1) [Cynara cardunculus var. scolymus]
          Length = 514

 Score =  143 bits (360), Expect = 3e-37
 Identities = 71/100 (71%), Positives = 82/100 (82%)
 Frame = -1

Query: 300 LPRYKFGKTLGIGSFGKVKLAVHTLTGLKVAIKILDRQSIDDCEAEKVRREIHIMRQLFH 121
           L  Y+ GKTLGIG+FGKVK A H LTG+KVAIKIL RQS  D  AEKVRREI+IMR L H
Sbjct: 13  LSNYRLGKTLGIGAFGKVKAAQHILTGVKVAIKILARQSFHDYAAEKVRREINIMRLLSH 72

Query: 120 PHVVRLFEVIDTPSHVYVVMEYMNSGELFYYLTENGKLHE 1
           PH+VRL+EVI+T S +YV+MEYMNSGELF Y+TEN +L E
Sbjct: 73  PHIVRLYEVIETRSAIYVIMEYMNSGELFDYITENSRLEE 112