BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00022305
         (591 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020550647.1| E4 SUMO-protein ligase PIAL2-like [Sesamum i...   233   4e-68
ref|XP_022867113.1| E4 SUMO-protein ligase PIAL2-like isoform X3...   175   6e-49
ref|XP_022867111.1| E4 SUMO-protein ligase PIAL1-like isoform X1...   175   9e-49
gb|PIN01560.1| hypothetical protein CDL12_25935 [Handroanthus im...   172   3e-46
ref|XP_011073310.1| E4 SUMO-protein ligase PIAL2 [Sesamum indicum]    170   2e-45
ref|XP_022880892.1| E4 SUMO-protein ligase PIAL2-like [Olea euro...   162   1e-42
gb|KZV15370.1| hypothetical protein F511_20355 [Dorcoceras hygro...   143   7e-36
emb|CDP12081.1| unnamed protein product [Coffea canephora]            140   6e-35
gb|PHT39733.1| hypothetical protein CQW23_18587 [Capsicum baccatum]   138   4e-34
ref|XP_016552263.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Cap...   137   7e-34
ref|XP_018629486.1| PREDICTED: uncharacterized protein LOC104105...   124   8e-31
ref|XP_016454178.1| PREDICTED: uncharacterized protein LOC107778...   124   4e-30
ref|XP_019247405.1| PREDICTED: E4 SUMO-protein ligase PIAL1-like...   125   7e-30
ref|XP_019247404.1| PREDICTED: E4 SUMO-protein ligase PIAL1-like...   125   7e-30
ref|XP_019247402.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   125   7e-30
ref|XP_019247401.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   125   7e-30
ref|XP_009783337.1| PREDICTED: uncharacterized protein LOC104231...   125   7e-30
gb|KZV31530.1| hypothetical protein F511_07381 [Dorcoceras hygro...   123   2e-29
ref|XP_016454176.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   124   3e-29
ref|XP_009612350.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   124   3e-29

>ref|XP_020550647.1| E4 SUMO-protein ligase PIAL2-like [Sesamum indicum]
          Length = 897

 Score =  233 bits (593), Expect = 4e-68
 Identities = 125/202 (61%), Positives = 147/202 (72%), Gaps = 5/202 (2%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGDGESPQPVDLLDLTQTNDGMDFV 180
           VEANVSDI+ SSDGSW AV +SD AIQKPE K S T + +SP+PVD+LDLTQT+D MD  
Sbjct: 393 VEANVSDIVFSSDGSWQAVKNSDAAIQKPEKKTSITENNDSPEPVDVLDLTQTDDAMDNT 452

Query: 181 TTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIFLSSLGQG- 357
            T ETEDRKLS+   Q+QS+TQT++V P IANT+ VN  S  VE DFWSG++LS+ G   
Sbjct: 453 ITCETEDRKLSQTACQSQSVTQTMAVNPLIANTNYVNTSSTDVEYDFWSGVYLSTFGLAS 512

Query: 358 ---PSNEQIGGVFVPTSNYIASPPVFTDSFTT-NGQVEGFNGNNLFTASVPQREPSLPNT 525
              PSNE +  V V TSN+IASPP  TDSF + NG+VE F GN+L  ASVPQ E SLP+T
Sbjct: 513 SNFPSNELVAAVSVSTSNFIASPPGLTDSFVSPNGEVEAFPGNSLMPASVPQSEASLPHT 572

Query: 526 LQLQQFQFGNSNYTAEYGRFPS 591
            QLQQ QFGN   T EYGR  S
Sbjct: 573 SQLQQCQFGNVTITNEYGRLQS 594


>ref|XP_022867113.1| E4 SUMO-protein ligase PIAL2-like isoform X3 [Olea europaea var.
           sylvestris]
 ref|XP_022867114.1| E4 SUMO-protein ligase PIAL2-like isoform X4 [Olea europaea var.
           sylvestris]
          Length = 507

 Score =  175 bits (444), Expect = 6e-49
 Identities = 95/206 (46%), Positives = 132/206 (64%), Gaps = 9/206 (4%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGD---------GESPQPVDLLDLT 153
           V  NV ++IISSDGSWNAV++++   +KPEDK S+ G          G    PV++LDLT
Sbjct: 8   VGPNVKEVIISSDGSWNAVIETEDTTKKPEDKTSNVGHDAPSEIESTGLPGAPVEILDLT 67

Query: 154 QTNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGI 333
           + +DGMD V T E+ED KLS    Q+ SMTQT SV P +ANT +VNQ    +++DFWSGI
Sbjct: 68  EIDDGMDAVATRESEDTKLSPTACQSLSMTQTPSVNPQMANTCDVNQNIGQIDEDFWSGI 127

Query: 334 FLSSLGQGPSNEQIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQREPS 513
           ++S+ G G            TSN  ++ P+   +  ++ +++ F+GN   + SVP+ E +
Sbjct: 128 YMSTFGTG------------TSNVRSNLPI---AGVSSRELDAFHGNVPLSTSVPESETA 172

Query: 514 LPNTLQLQQFQFGNSNYTAEYGRFPS 591
           LPN LQLQQ+QFGNSN + EYGR PS
Sbjct: 173 LPNILQLQQYQFGNSNMSNEYGRLPS 198


>ref|XP_022867111.1| E4 SUMO-protein ligase PIAL1-like isoform X1 [Olea europaea var.
           sylvestris]
 ref|XP_022867112.1| E4 SUMO-protein ligase PIAL1-like isoform X2 [Olea europaea var.
           sylvestris]
          Length = 525

 Score =  175 bits (444), Expect = 9e-49
 Identities = 95/206 (46%), Positives = 132/206 (64%), Gaps = 9/206 (4%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGD---------GESPQPVDLLDLT 153
           V  NV ++IISSDGSWNAV++++   +KPEDK S+ G          G    PV++LDLT
Sbjct: 8   VGPNVKEVIISSDGSWNAVIETEDTTKKPEDKTSNVGHDAPSEIESTGLPGAPVEILDLT 67

Query: 154 QTNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGI 333
           + +DGMD V T E+ED KLS    Q+ SMTQT SV P +ANT +VNQ    +++DFWSGI
Sbjct: 68  EIDDGMDAVATRESEDTKLSPTACQSLSMTQTPSVNPQMANTCDVNQNIGQIDEDFWSGI 127

Query: 334 FLSSLGQGPSNEQIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQREPS 513
           ++S+ G G            TSN  ++ P+   +  ++ +++ F+GN   + SVP+ E +
Sbjct: 128 YMSTFGTG------------TSNVRSNLPI---AGVSSRELDAFHGNVPLSTSVPESETA 172

Query: 514 LPNTLQLQQFQFGNSNYTAEYGRFPS 591
           LPN LQLQQ+QFGNSN + EYGR PS
Sbjct: 173 LPNILQLQQYQFGNSNMSNEYGRLPS 198


>gb|PIN01560.1| hypothetical protein CDL12_25935 [Handroanthus impetiginosus]
          Length = 891

 Score =  172 bits (436), Expect = 3e-46
 Identities = 101/202 (50%), Positives = 124/202 (61%), Gaps = 5/202 (2%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGDGESPQPVDLLDLTQTNDGMDFV 180
           V ANVS I +SSDGSW+AVM+S+  IQKPED   +    +S QP D+LDLTQ +D MD V
Sbjct: 390 VGANVSVITVSSDGSWSAVMESEETIQKPEDNTLNPEQDDSHQPADVLDLTQNDDAMDAV 449

Query: 181 TTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIFLSSLG--- 351
              ETEDRK    +   Q +TQT  V PH+ANT++V Q      DDFWSGI + + G   
Sbjct: 450 AAYETEDRKQFPNLHGGQFVTQTTVVNPHMANTNDVTQ------DDFWSGIHMPTFGLVS 503

Query: 352 -QGPSNEQIGGVFVPTSNYIASPPVFTDSFTT-NGQVEGFNGNNLFTASVPQREPSLPNT 525
               SN QIG       ++ AS  V  DSFT+ +   E F+G+ L   SVPQ E SLPNT
Sbjct: 504 PNVRSNAQIG-------SFSASTSVLADSFTSPSTNAEAFHGSALVATSVPQGETSLPNT 556

Query: 526 LQLQQFQFGNSNYTAEYGRFPS 591
           +Q QQ+Q GN   T EYGRFPS
Sbjct: 557 MQTQQYQIGNPAITNEYGRFPS 578


>ref|XP_011073310.1| E4 SUMO-protein ligase PIAL2 [Sesamum indicum]
          Length = 886

 Score =  170 bits (430), Expect = 2e-45
 Identities = 101/199 (50%), Positives = 126/199 (63%), Gaps = 5/199 (2%)
 Frame = +1

Query: 10  NVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGDGESPQPVDLLDLTQTNDGMDFVTTN 189
           NVS I++SSDGSWNAVM+ DT +Q PED+  + G+ E  QP D+LDLTQT+D +  ++  
Sbjct: 394 NVSTIVVSSDGSWNAVMEEDT-VQNPEDETFNAGEDEFSQPRDVLDLTQTDDAIYAISAC 452

Query: 190 ETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIFLSSLGQG-PSN 366
           E EDRK     +  Q M QT ++ PHIANT++VNQ S HV +DF SGI+  ++G G PSN
Sbjct: 453 EPEDRKHFSD-ADLQFMDQTEAINPHIANTNDVNQSSTHVANDFRSGIYTPTIGLGLPSN 511

Query: 367 EQIGGVFVPTSNYIASP---PVFTDSFTTNGQ-VEGFNGNNLFTASVPQREPSLPNTLQL 534
                 F P  +    P      T  FT+  Q VE F GN L TAS  Q   SLPNT QL
Sbjct: 512 ------FRPNPDIAGCPVSISAITHPFTSPSQEVEAFRGNALVTASTSQTATSLPNTSQL 565

Query: 535 QQFQFGNSNYTAEYGRFPS 591
           QQ+QFGN + T EYGRFPS
Sbjct: 566 QQYQFGNPSITNEYGRFPS 584


>ref|XP_022880892.1| E4 SUMO-protein ligase PIAL2-like [Olea europaea var. sylvestris]
          Length = 854

 Score =  162 bits (410), Expect = 1e-42
 Identities = 92/206 (44%), Positives = 123/206 (59%), Gaps = 9/206 (4%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGD---------GESPQPVDLLDLT 153
           V  N  ++IISSDGSWNAVMD+D A ++PE+K  + G          G +   V++LDLT
Sbjct: 341 VGPNAKEVIISSDGSWNAVMDTDDATKEPEEKTFNVGQDVPRGIESAGLADASVEILDLT 400

Query: 154 QTNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGI 333
           + +D  D V T E+EDRKLS    Q+ S+TQT SV P +ANT++VNQ  A  +DDFWSGI
Sbjct: 401 EIDDVTDAVATRESEDRKLSPTAHQSLSITQTPSVNPQMANTNDVNQNIAQTDDDFWSGI 460

Query: 334 FLSSLGQGPSNEQIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQREPS 513
           FLS+   G            T N  ++PP+   +  +N +++ F GN   T  V + E +
Sbjct: 461 FLSTYEMG------------TLNVRSNPPI---TSVSNWELDAFQGNIPLTTFVMESETA 505

Query: 514 LPNTLQLQQFQFGNSNYTAEYGRFPS 591
           LPN  QLQQ+QFGN N   EYG  PS
Sbjct: 506 LPNISQLQQYQFGNPNMGNEYGELPS 531


>gb|KZV15370.1| hypothetical protein F511_20355 [Dorcoceras hygrometricum]
          Length = 2269

 Score =  143 bits (360), Expect = 7e-36
 Identities = 91/209 (43%), Positives = 122/209 (58%), Gaps = 13/209 (6%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGDGESPQ---------PVDLLDLT 153
           V  NV+D+I +SDGSW+AVM +D  I  PED+ S++G  ESP+         PV+++DLT
Sbjct: 333 VGKNVTDVIFASDGSWSAVMATDDNIHTPEDRTSNSGQNESPETESVSLSNVPVEIIDLT 392

Query: 154 QTNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGI 333
           Q ND MD VT NETED+KLS  I+Q++S+TQT+ V+P+  NT++VNQ +A  +DD W  I
Sbjct: 393 Q-NDSMD-VTPNETEDQKLSS-IAQSRSLTQTVVVDPYRTNTNDVNQSNACAKDDTWFRI 449

Query: 334 FLSSLGQG----PSNEQIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQ 501
           F+S+ G G     +N Q    +  T N+IA+P V  +   T                   
Sbjct: 450 FMSTFGPGVPDLTTNTQNLHAYASTLNHIAAPAVLPEIPVT------------------- 490

Query: 502 REPSLPNTLQLQQFQFGNSNYTAEYGRFP 588
              SLPNTL  QQ Q GN   T EY RFP
Sbjct: 491 ---SLPNTLLAQQNQVGNPAMTNEYERFP 516


>emb|CDP12081.1| unnamed protein product [Coffea canephora]
          Length = 929

 Score =  140 bits (353), Expect = 6e-35
 Identities = 85/205 (41%), Positives = 117/205 (57%), Gaps = 14/205 (6%)
 Frame = +1

Query: 10   NVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGDGESPQ---------PVDLLDLTQTN 162
            NV+D+IISSDGSW A+++SD   +K +DK       +  Q         P DLLDLT+ +
Sbjct: 390  NVNDVIISSDGSWKAIVESDDHAEKRQDKFPSAEQEQPTQPDSTSLPNAPPDLLDLTEID 449

Query: 163  DGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQR-SAHVEDDFWSGIFL 339
            D MD V  +E ED K+  + SQ     + +++ P    T+ V Q  S+  EDDFWSG++L
Sbjct: 450  DVMDTVDLSEAEDTKVFLVNSQKDCSIKDMTLRPPTNITNEVPQNSSSQTEDDFWSGVYL 509

Query: 340  SSLGQGP----SNEQIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQRE 507
            S+ G G     S+ Q GGV   TS+ I   P+ TD+ +    VE    N   + SVPQ E
Sbjct: 510  STFGSGTFSLMSDAQSGGVPQSTSSSILPSPLLTDASSPASNVEARASNAFLSNSVPQTE 569

Query: 508  PSLPNTLQLQQFQFGNSNYTAEYGR 582
             S P  LQLQ+FQFGN++ + EYGR
Sbjct: 570  IS-PTALQLQRFQFGNASISNEYGR 593


>gb|PHT39733.1| hypothetical protein CQW23_18587 [Capsicum baccatum]
          Length = 859

 Score =  138 bits (347), Expect = 4e-34
 Identities = 87/205 (42%), Positives = 113/205 (55%), Gaps = 8/205 (3%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDT--------GDGESPQPVDLLDLTQ 156
           V  +V+D++ISSDGSW AVM+SD   +KP DK  D          DG S  P D+LDLT+
Sbjct: 386 VSEDVTDVVISSDGSWKAVMESDDRTEKPRDKTPDIAQDSPHRGSDGPSNAPSDVLDLTE 445

Query: 157 TNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIF 336
            +D M  VTT ETEDRK    ISQ QS  Q+ +V   + N   +NQ    + DDFWS ++
Sbjct: 446 MDDEMYPVTTAETEDRKNFPAISQIQSNVQSTTV---VNNPSEINQTGPGMTDDFWSRLY 502

Query: 337 LSSLGQGPSNEQIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQREPSL 516
           LSSL  G ++E       P    +   PVFTD+             ++   +V + E + 
Sbjct: 503 LSSLQTGSASE-------PARINLLQLPVFTDAIPP---------TSIIPTAVLENELAA 546

Query: 517 PNTLQLQQFQFGNSNYTAEYGRFPS 591
            N LQLQQFQFGNS  + EYGRF S
Sbjct: 547 SNMLQLQQFQFGNSAISNEYGRFAS 571


>ref|XP_016552263.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Capsicum annuum]
          Length = 856

 Score =  137 bits (345), Expect = 7e-34
 Identities = 87/205 (42%), Positives = 113/205 (55%), Gaps = 8/205 (3%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDT--------GDGESPQPVDLLDLTQ 156
           V  +V+D++ISSDGSW AVM+SD   +KP DK  D          DG S  P D+LDLT+
Sbjct: 383 VSEDVTDVVISSDGSWKAVMESDDRTEKPRDKTPDIAQDSPHRGSDGPSNAPSDVLDLTE 442

Query: 157 TNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIF 336
            +D M  VTT ETEDRK    ISQ QS  Q+ +V   + N   +NQ    + DDFWS ++
Sbjct: 443 MDDEMYPVTTAETEDRKNFPAISQIQSNVQSTTV---VNNPSELNQTGPGMTDDFWSRLY 499

Query: 337 LSSLGQGPSNEQIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQREPSL 516
           LSSL  G ++E       P    +   PVFTD+             ++   +V + E + 
Sbjct: 500 LSSLQTGSASE-------PARINLLQLPVFTDAIPP---------TSIIPTAVLENELAA 543

Query: 517 PNTLQLQQFQFGNSNYTAEYGRFPS 591
            N LQLQQFQFGNS  + EYGRF S
Sbjct: 544 SNMLQLQQFQFGNSAISNEYGRFAS 568


>ref|XP_018629486.1| PREDICTED: uncharacterized protein LOC104105686 isoform X1
           [Nicotiana tomentosiformis]
          Length = 315

 Score =  124 bits (310), Expect = 8e-31
 Identities = 84/204 (41%), Positives = 111/204 (54%), Gaps = 10/204 (4%)
 Frame = +1

Query: 10  NVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTG--------DGESPQPVDLLDLTQTND 165
           +V D++ISS GSW A+M+S+   +KP+DK  D          DG S    D+LDLT+ +D
Sbjct: 11  DVIDVMISSVGSWKAIMESNDHTEKPKDKTPDFAQDSSQRGSDGLSNAHSDVLDLTEIDD 70

Query: 166 GMDFVTTNETEDRK-LSRIISQNQSMTQTISVEPHIANTDNVNQRSA-HVEDDFWSGIFL 339
            M+ + T ETEDRK L+ I  QN +          + N   ++Q  A ++ DDFW GI+L
Sbjct: 71  EMNPMITAETEDRKNLASINVQNTTA---------VINPCEISQTGASYLADDFWPGIYL 121

Query: 340 SSLGQGPSNEQIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQREPSLP 519
           +SL       Q GG   P    +  PPV TD+ +     EG   N L    V Q   S P
Sbjct: 122 ASL-------QTGGASEPARTNLLQPPVLTDAISPALNPEG---NGLIPTFVLQSGLSSP 171

Query: 520 NTLQLQQFQFGNSNYTAEYGRFPS 591
           N LQLQQFQFGNS+ + EYG FPS
Sbjct: 172 NILQLQQFQFGNSSISNEYGMFPS 195


>ref|XP_016454178.1| PREDICTED: uncharacterized protein LOC107778436 [Nicotiana tabacum]
          Length = 417

 Score =  124 bits (310), Expect = 4e-30
 Identities = 84/204 (41%), Positives = 111/204 (54%), Gaps = 10/204 (4%)
 Frame = +1

Query: 10  NVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTG--------DGESPQPVDLLDLTQTND 165
           +V D++ISS GSW A+M+S+   +KP+DK  D          DG S    D+LDLT+ +D
Sbjct: 46  DVIDVMISSVGSWKAIMESNDHTEKPKDKTPDFAQDSSQRGSDGLSNAHSDVLDLTEIDD 105

Query: 166 GMDFVTTNETEDRK-LSRIISQNQSMTQTISVEPHIANTDNVNQRSA-HVEDDFWSGIFL 339
            M+ + T ETEDRK L+ I  QN +          + N   ++Q  A ++ DDFW GI+L
Sbjct: 106 EMNPMITAETEDRKNLASINVQNTTA---------VINPCEISQTGASYLADDFWPGIYL 156

Query: 340 SSLGQGPSNEQIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQREPSLP 519
           +SL       Q GG   P    +  PPV TD+ +     EG   N L    V Q   S P
Sbjct: 157 ASL-------QTGGASEPARTNLLQPPVLTDAISPALNPEG---NGLIPTFVLQSGLSSP 206

Query: 520 NTLQLQQFQFGNSNYTAEYGRFPS 591
           N LQLQQFQFGNS+ + EYG FPS
Sbjct: 207 NILQLQQFQFGNSSISNEYGMFPS 230


>ref|XP_019247405.1| PREDICTED: E4 SUMO-protein ligase PIAL1-like isoform X4 [Nicotiana
           attenuata]
          Length = 778

 Score =  125 bits (315), Expect = 7e-30
 Identities = 80/209 (38%), Positives = 112/209 (53%), Gaps = 12/209 (5%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDLLDLTQ 156
           V  +V+D++ISSDGSW A+M+SD   +KP DKI D          D  S  P D++DLT 
Sbjct: 284 VGEDVTDVMISSDGSWKAIMESDDHSEKPRDKIPDFTQDSPQRGSDSFSNTPADVMDLTA 343

Query: 157 TNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIF 336
            +D ++ + T+ETED K    I    S  Q  +V  + +  D+     + + DDFWS ++
Sbjct: 344 IDDEINPMATSETEDSKNFPSIPNIHSNVQNTTVVNNPSEIDHTG--GSDMADDFWSRMY 401

Query: 337 LSSLGQGPSNE----QIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQR 504
           +SS G G S      Q G    P+   +  PPV TD+ +     E   GN L   S+ + 
Sbjct: 402 MSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPAFNPE---GNALIPTSILES 458

Query: 505 EPSLPNTLQLQQFQFGNSNYTAEYGRFPS 591
             S  + +QLQQFQFGNS  + EYGRFPS
Sbjct: 459 GLSSSDMMQLQQFQFGNSAISNEYGRFPS 487


>ref|XP_019247404.1| PREDICTED: E4 SUMO-protein ligase PIAL1-like isoform X3 [Nicotiana
           attenuata]
          Length = 782

 Score =  125 bits (315), Expect = 7e-30
 Identities = 80/209 (38%), Positives = 112/209 (53%), Gaps = 12/209 (5%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDLLDLTQ 156
           V  +V+D++ISSDGSW A+M+SD   +KP DKI D          D  S  P D++DLT 
Sbjct: 284 VGEDVTDVMISSDGSWKAIMESDDHSEKPRDKIPDFTQDSPQRGSDSFSNTPADVMDLTA 343

Query: 157 TNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIF 336
            +D ++ + T+ETED K    I    S  Q  +V  + +  D+     + + DDFWS ++
Sbjct: 344 IDDEINPMATSETEDSKNFPSIPNIHSNVQNTTVVNNPSEIDHTG--GSDMADDFWSRMY 401

Query: 337 LSSLGQGPSNE----QIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQR 504
           +SS G G S      Q G    P+   +  PPV TD+ +     E   GN L   S+ + 
Sbjct: 402 MSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPAFNPE---GNALIPTSILES 458

Query: 505 EPSLPNTLQLQQFQFGNSNYTAEYGRFPS 591
             S  + +QLQQFQFGNS  + EYGRFPS
Sbjct: 459 GLSSSDMMQLQQFQFGNSAISNEYGRFPS 487


>ref|XP_019247402.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X2 [Nicotiana
           attenuata]
          Length = 789

 Score =  125 bits (315), Expect = 7e-30
 Identities = 80/209 (38%), Positives = 112/209 (53%), Gaps = 12/209 (5%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDLLDLTQ 156
           V  +V+D++ISSDGSW A+M+SD   +KP DKI D          D  S  P D++DLT 
Sbjct: 284 VGEDVTDVMISSDGSWKAIMESDDHSEKPRDKIPDFTQDSPQRGSDSFSNTPADVMDLTA 343

Query: 157 TNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIF 336
            +D ++ + T+ETED K    I    S  Q  +V  + +  D+     + + DDFWS ++
Sbjct: 344 IDDEINPMATSETEDSKNFPSIPNIHSNVQNTTVVNNPSEIDHTG--GSDMADDFWSRMY 401

Query: 337 LSSLGQGPSNE----QIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQR 504
           +SS G G S      Q G    P+   +  PPV TD+ +     E   GN L   S+ + 
Sbjct: 402 MSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPAFNPE---GNALIPTSILES 458

Query: 505 EPSLPNTLQLQQFQFGNSNYTAEYGRFPS 591
             S  + +QLQQFQFGNS  + EYGRFPS
Sbjct: 459 GLSSSDMMQLQQFQFGNSAISNEYGRFPS 487


>ref|XP_019247401.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X1 [Nicotiana
           attenuata]
 gb|OIT02146.1| e4 sumo-protein ligase pial2 [Nicotiana attenuata]
          Length = 793

 Score =  125 bits (315), Expect = 7e-30
 Identities = 80/209 (38%), Positives = 112/209 (53%), Gaps = 12/209 (5%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDLLDLTQ 156
           V  +V+D++ISSDGSW A+M+SD   +KP DKI D          D  S  P D++DLT 
Sbjct: 284 VGEDVTDVMISSDGSWKAIMESDDHSEKPRDKIPDFTQDSPQRGSDSFSNTPADVMDLTA 343

Query: 157 TNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIF 336
            +D ++ + T+ETED K    I    S  Q  +V  + +  D+     + + DDFWS ++
Sbjct: 344 IDDEINPMATSETEDSKNFPSIPNIHSNVQNTTVVNNPSEIDHTG--GSDMADDFWSRMY 401

Query: 337 LSSLGQGPSNE----QIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQR 504
           +SS G G S      Q G    P+   +  PPV TD+ +     E   GN L   S+ + 
Sbjct: 402 MSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPAFNPE---GNALIPTSILES 458

Query: 505 EPSLPNTLQLQQFQFGNSNYTAEYGRFPS 591
             S  + +QLQQFQFGNS  + EYGRFPS
Sbjct: 459 GLSSSDMMQLQQFQFGNSAISNEYGRFPS 487


>ref|XP_009783337.1| PREDICTED: uncharacterized protein LOC104231952 [Nicotiana
           sylvestris]
 ref|XP_016457244.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X1 [Nicotiana
           tabacum]
          Length = 882

 Score =  125 bits (315), Expect = 7e-30
 Identities = 81/210 (38%), Positives = 112/210 (53%), Gaps = 13/210 (6%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDLLDLTQ 156
           V  +V+D++ISSDGSW A+M+SD   +KP DKI D          DG S  P D++DLT 
Sbjct: 385 VGEDVTDVMISSDGSWKAIMESDDHTEKPRDKIPDFTQDSPQRGSDGFSNTPADVMDLTD 444

Query: 157 TNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQR-SAHVEDDFWSGI 333
            +D ++ + T ETED K    I    S  Q  +V   + N   +N    + + DDFWS +
Sbjct: 445 IDDEINPMATCETEDSKNFPSIPNIHSNVQNTTV---VNNPSEINHTGGSDMADDFWSRM 501

Query: 334 FLSSLGQGPSNE----QIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQ 501
           ++SS G G S      Q G    P+   +  PPV TD+ + +   E   GN L   S+ +
Sbjct: 502 YMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPSFNPE---GNALIPTSLLE 558

Query: 502 REPSLPNTLQLQQFQFGNSNYTAEYGRFPS 591
              S  + +QLQ FQFGNS  + EYGRFPS
Sbjct: 559 SGLSSSDMMQLQHFQFGNSAVSNEYGRFPS 588


>gb|KZV31530.1| hypothetical protein F511_07381 [Dorcoceras hygrometricum]
          Length = 534

 Score =  123 bits (309), Expect = 2e-29
 Identities = 79/211 (37%), Positives = 111/211 (52%), Gaps = 14/211 (6%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGDGESPQ---------PVDLLDLT 153
           V AN +++I +SDGSWN    +D + QK  D IS     E PQ         P D+LDLT
Sbjct: 35  VGANDTEVIFASDGSWNVGTGNDDSTQKSMDNISINAQNEPPQSESVPLSKAPADVLDLT 94

Query: 154 QTNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGI 333
             +D +  ++ +E +D  L+    + Q+ T T++V+    + ++ NQ +  ++DDFWSG+
Sbjct: 95  AIDDAICVISDDEIQDMALNPTY-KCQTETPTVAVDSQKTSKNDGNQSNTCLDDDFWSGL 153

Query: 334 FLSSLGQ--GPSNEQIGGVFVPTSNYIASPPVFTDSFTTNG---QVEGFNGNNLFTASVP 498
            L +  +    SN  I GV   TSN I    VFT          ++E    + L TASVP
Sbjct: 154 VLPNFFEVSSQSNSPIDGVSATTSNKIVQTSVFTQPVGPKAPTQELESIYTDALLTASVP 213

Query: 499 QREPSLPNTLQLQQFQFGNSNYTAEYGRFPS 591
           +   SL  TL  QQ QFG S  T EYGRFPS
Sbjct: 214 RTVTSLSTTLPFQQHQFGTSAITNEYGRFPS 244


>ref|XP_016454176.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X2 [Nicotiana
           tabacum]
          Length = 890

 Score =  124 bits (310), Expect = 3e-29
 Identities = 81/210 (38%), Positives = 112/210 (53%), Gaps = 13/210 (6%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDLLDLTQ 156
           V  +V+D++ISSDGSW A+M+SD   +K  DKI D          DG S  P D++DLT 
Sbjct: 385 VGEDVTDVMISSDGSWKAIMESDDHTEKSRDKIPDFTQDSPQRGSDGFSNTPADVMDLTD 444

Query: 157 TNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQR-SAHVEDDFWSGI 333
            +D ++ + T+ETED K    I    S  Q  +V   + N   +N    + + DDFWS +
Sbjct: 445 IDDEINPMATSETEDSKNFPSIPNIHSNGQNTTV---VNNPSEINHTGGSDMADDFWSRM 501

Query: 334 FLSSLGQGPSNE----QIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQ 501
           ++SS G G S      Q G    P+   +  PPV TD+ +     E   GN L   S+ +
Sbjct: 502 YMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPALNPE---GNALIPTSILE 558

Query: 502 REPSLPNTLQLQQFQFGNSNYTAEYGRFPS 591
              S  + +QLQQFQFGNS  + EYGRFPS
Sbjct: 559 CGLSSSDLMQLQQFQFGNSAISNEYGRFPS 588


>ref|XP_009612350.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X2 [Nicotiana
           tomentosiformis]
          Length = 890

 Score =  124 bits (310), Expect = 3e-29
 Identities = 81/210 (38%), Positives = 112/210 (53%), Gaps = 13/210 (6%)
 Frame = +1

Query: 1   VEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDLLDLTQ 156
           V  +V+D++ISSDGSW A+M+SD   +K  DKI D          DG S  P D++DLT 
Sbjct: 385 VGEDVTDVMISSDGSWKAIMESDDHTEKSRDKIPDFTQDSPQRGSDGFSNTPADVMDLTD 444

Query: 157 TNDGMDFVTTNETEDRKLSRIISQNQSMTQTISVEPHIANTDNVNQR-SAHVEDDFWSGI 333
            +D ++ + T+ETED K    I    S  Q  +V   + N   +N    + + DDFWS +
Sbjct: 445 IDDEINPMATSETEDSKNFPSIPNIHSNGQNTTV---VNNPSEINHTGGSDMADDFWSRM 501

Query: 334 FLSSLGQGPSNE----QIGGVFVPTSNYIASPPVFTDSFTTNGQVEGFNGNNLFTASVPQ 501
           ++SS G G S      Q G    P+   +  PPV TD+ +     E   GN L   S+ +
Sbjct: 502 YMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPALNPE---GNALIPTSILE 558

Query: 502 REPSLPNTLQLQQFQFGNSNYTAEYGRFPS 591
              S  + +QLQQFQFGNS  + EYGRFPS
Sbjct: 559 CGLSSSDLMQLQQFQFGNSAISNEYGRFPS 588