BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00016722
         (522 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PIN24475.1| Acyl-CoA synthetase [Handroanthus impetiginosus]       238   4e-77
ref|XP_020548878.1| LOW QUALITY PROTEIN: 4-coumarate--CoA ligase...   234   8e-77
gb|PIN04540.1| Acyl-CoA synthetase [Handroanthus impetiginosus]       228   3e-73
gb|KZV33003.1| 4-coumarate--CoA ligase-like 5 [Dorcoceras hygrom...   223   2e-72
ref|XP_012843328.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [E...   215   3e-69
ref|XP_022841470.1| 4-coumarate--CoA ligase-like 5 [Olea europae...   216   2e-68
gb|KVI09677.1| AMP-binding, conserved site-containing protein [C...   206   7e-67
ref|XP_023751376.1| 4-coumarate--CoA ligase 1-like [Lactuca sati...   204   3e-66
ref|XP_010090435.1| 4-coumarate--CoA ligase-like 5 [Morus notabi...   197   3e-64
gb|KVI05000.1| AMP-binding, conserved site-containing protein [C...   203   9e-64
ref|XP_002271586.3| PREDICTED: 4-coumarate--CoA ligase-like 5 [V...   199   3e-63
ref|XP_019198798.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [I...   204   4e-63
ref|XP_023762700.1| 4-coumarate--CoA ligase 1-like [Lactuca sativa]   202   6e-63
gb|PLY86340.1| hypothetical protein LSAT_8X17560 [Lactuca sativa]     202   6e-63
ref|XP_007219482.1| 4-coumarate--CoA ligase-like 5 [Prunus persi...   197   1e-62
ref|XP_021818824.1| 4-coumarate--CoA ligase-like 5 isoform X1 [P...   194   3e-62
ref|XP_021818825.1| 4-coumarate--CoA ligase-like 5 isoform X2 [P...   194   3e-62
ref|XP_009350226.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [P...   195   8e-62
ref|XP_009361476.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [P...   195   8e-62
ref|XP_021993469.1| 4-coumarate--CoA ligase-like 5 [Helianthus a...   204   8e-62

>gb|PIN24475.1| Acyl-CoA synthetase [Handroanthus impetiginosus]
          Length = 558

 Score =  238 bits (607), Expect(2) = 4e-77
 Identities = 115/133 (86%), Positives = 119/133 (89%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVILG VKYNG GYDLSSLRR+GSGAAPLSKE A+ FR KFPWV LR GYGLTESCG
Sbjct: 297 AVPPVILGLVKYNGGGYDLSSLRRVGSGAAPLSKEVADEFRTKFPWVNLRTGYGLTESCG 356

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           A TFFVS+EEAKARP S ARLAPCFSAKVVDFETGL LPPYKEGELW+KSPTVMKEYLGN
Sbjct: 357 AGTFFVSDEEAKARPGSVARLAPCFSAKVVDFETGLALPPYKEGELWLKSPTVMKEYLGN 416

Query: 484 EAANAAAFDSEGW 522
           E A AA FDSEGW
Sbjct: 417 EEATAATFDSEGW 429



 Score = 78.2 bits (191), Expect(2) = 4e-77
 Identities = 38/49 (77%), Positives = 41/49 (83%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLFSRTSGYTRV 149
           SV+TLLKWSVDVSKA DD+FLCFIP+FHIYGL FFGLGL    SG T V
Sbjct: 227 SVITLLKWSVDVSKAQDDIFLCFIPMFHIYGLVFFGLGL--PCSGITTV 273


>ref|XP_020548878.1| LOW QUALITY PROTEIN: 4-coumarate--CoA ligase-like 5 [Sesamum
           indicum]
          Length = 578

 Score =  234 bits (596), Expect(2) = 8e-77
 Identities = 112/133 (84%), Positives = 119/133 (89%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVILG VKYNG GY+LSSLRR+GSGAAPLSKE A+ FR KFPWVELRPGYGLTESCG
Sbjct: 303 AVPPVILGLVKYNGGGYNLSSLRRVGSGAAPLSKEVADAFRVKFPWVELRPGYGLTESCG 362

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           AATFF SN+EAKARP SSARL PCF+AKVVDFETGL LPP +EGELW+KSPTVMKEYLGN
Sbjct: 363 AATFFASNQEAKARPGSSARLLPCFTAKVVDFETGLELPPNREGELWLKSPTVMKEYLGN 422

Query: 484 EAANAAAFDSEGW 522
             A AA FDSEGW
Sbjct: 423 AEATAATFDSEGW 435



 Score = 81.3 bits (199), Expect(2) = 8e-77
 Identities = 36/40 (90%), Positives = 39/40 (97%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLF 122
           SV+TLLKWSVDVSKA+DDVFLCFIP+FHIYGL FFGLGLF
Sbjct: 233 SVITLLKWSVDVSKAHDDVFLCFIPLFHIYGLVFFGLGLF 272


>gb|PIN04540.1| Acyl-CoA synthetase [Handroanthus impetiginosus]
          Length = 562

 Score =  228 bits (580), Expect(2) = 3e-73
 Identities = 110/133 (82%), Positives = 117/133 (87%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVILG VK+ G GYDLSSLRR+GSGAAPLSKE  +GFRKKFPWVELRPGYGLTESCG
Sbjct: 301 AVPPVILGLVKHGGGGYDLSSLRRVGSGAAPLSKEVVDGFRKKFPWVELRPGYGLTESCG 360

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           AATFF S+ EAKARPASSARL P FSAKVVD ETGL LPPY+EGELW+KSPTVMKEYLGN
Sbjct: 361 AATFFASDAEAKARPASSARLLPGFSAKVVDIETGLALPPYREGELWLKSPTVMKEYLGN 420

Query: 484 EAANAAAFDSEGW 522
           E A AA  DS+ W
Sbjct: 421 EEATAATIDSDRW 433



 Score = 75.5 bits (184), Expect(2) = 3e-73
 Identities = 35/41 (85%), Positives = 36/41 (87%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLFS 125
           SV  LLKWSVDVSKA DDV LCFIP+FHIYGL FFGLGLFS
Sbjct: 231 SVFRLLKWSVDVSKAYDDVLLCFIPLFHIYGLVFFGLGLFS 271


>gb|KZV33003.1| 4-coumarate--CoA ligase-like 5 [Dorcoceras hygrometricum]
          Length = 592

 Score =  223 bits (569), Expect(2) = 2e-72
 Identities = 108/133 (81%), Positives = 117/133 (87%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVILG VKY+G GYDLSSLRR+GSGAAPLSKE A+ FR+KFPWVELRPGYGLTESCG
Sbjct: 309 AVPPVILGLVKYDGGGYDLSSLRRVGSGAAPLSKELADRFREKFPWVELRPGYGLTESCG 368

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           A TFF S+ EAKARPASSARLAPCFSAKVVD ETG  LPP +EGELW++SPTVMKEYLGN
Sbjct: 369 AGTFFESHGEAKARPASSARLAPCFSAKVVDLETGFCLPPNREGELWLRSPTVMKEYLGN 428

Query: 484 EAANAAAFDSEGW 522
             A AA  DS+GW
Sbjct: 429 VEATAATLDSDGW 441



 Score = 77.4 bits (189), Expect(2) = 2e-72
 Identities = 34/39 (87%), Positives = 38/39 (97%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGL 119
           SV+TLLKWSV+VSKA DDVFLCF+P+FHIYGLAFFGLGL
Sbjct: 239 SVITLLKWSVEVSKAQDDVFLCFLPMFHIYGLAFFGLGL 277


>ref|XP_012843328.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Erythranthe guttata]
 gb|EYU32443.1| hypothetical protein MIMGU_mgv1a019706mg [Erythranthe guttata]
          Length = 563

 Score =  215 bits (547), Expect(2) = 3e-69
 Identities = 109/135 (80%), Positives = 114/135 (84%), Gaps = 2/135 (1%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKG--YDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTES 297
           AVP VILG VKYNG G  YDLSSLRR+GSGAAPLSKE    FR KFP VELR GYGLTES
Sbjct: 300 AVPSVILGLVKYNGGGRHYDLSSLRRVGSGAAPLSKEVIGKFRMKFPRVELRSGYGLTES 359

Query: 298 CGAATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYL 477
           CGA T F S+EEA+ARPASSARL PCF AKVVDFETGL LPPYKEGELW+KSPTVMK YL
Sbjct: 360 CGATTLFSSDEEAEARPASSARLLPCFKAKVVDFETGLALPPYKEGELWLKSPTVMKGYL 419

Query: 478 GNEAANAAAFDSEGW 522
           GNE A AA+FDSEGW
Sbjct: 420 GNEEATAASFDSEGW 434



 Score = 75.1 bits (183), Expect(2) = 3e-69
 Identities = 34/39 (87%), Positives = 36/39 (92%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGL 119
           SVVTLLKWSVDV+ A  D+FLCFIPIFHIYGLAFFGLGL
Sbjct: 230 SVVTLLKWSVDVTNARKDIFLCFIPIFHIYGLAFFGLGL 268


>ref|XP_022841470.1| 4-coumarate--CoA ligase-like 5 [Olea europaea var. sylvestris]
          Length = 562

 Score =  216 bits (550), Expect(2) = 2e-68
 Identities = 104/133 (78%), Positives = 114/133 (85%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVILG VKY G   DLSSLRR+GSGAAPLSKE A+ FR+KFPWVELRPGYGLTES G
Sbjct: 302 AVPPVILGLVKYPGGANDLSSLRRVGSGAAPLSKEVADRFREKFPWVELRPGYGLTESTG 361

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
            AT+FVSN +AKAR ASS RL PCFSAKVVD ETG  LPP+K+GELW+KSPT+MKEYLGN
Sbjct: 362 GATYFVSNNQAKARSASSGRLIPCFSAKVVDVETGFTLPPHKQGELWLKSPTIMKEYLGN 421

Query: 484 EAANAAAFDSEGW 522
           E A AA FDS+GW
Sbjct: 422 EDATAATFDSDGW 434



 Score = 70.9 bits (172), Expect(2) = 2e-68
 Identities = 31/40 (77%), Positives = 35/40 (87%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLF 122
           S++ LLKWSVDVS A  DVFLCFIP+FHIYGLAFF LG+F
Sbjct: 232 SIMKLLKWSVDVSNAQKDVFLCFIPMFHIYGLAFFALGMF 271


>gb|KVI09677.1| AMP-binding, conserved site-containing protein [Cynara cardunculus
           var. scolymus]
          Length = 534

 Score =  206 bits (524), Expect(2) = 7e-67
 Identities = 98/133 (73%), Positives = 109/133 (81%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVIL  VKY G GYDLSSLRR+GSGAAPLSKE  + FR KFPWV LRPGYGLTESCG
Sbjct: 299 AVPPVILNLVKYKG-GYDLSSLRRVGSGAAPLSKELVDAFRLKFPWVALRPGYGLTESCG 357

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           AAT F+SNEEAK+ PA+S  L P F AKVVD+ETG+ +PPY+EGELW+  P VMK YLGN
Sbjct: 358 AATLFISNEEAKSHPAASGTLLPTFCAKVVDYETGIAMPPYREGELWLNGPAVMKGYLGN 417

Query: 484 EAANAAAFDSEGW 522
           EAA AA  DS+GW
Sbjct: 418 EAATAATIDSDGW 430



 Score = 75.9 bits (185), Expect(2) = 7e-67
 Identities = 34/40 (85%), Positives = 38/40 (95%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLF 122
           SV+TLLKWSVD + AN+DVFLCFIP+FHIYGLAFFGLGLF
Sbjct: 229 SVMTLLKWSVDNTSANNDVFLCFIPMFHIYGLAFFGLGLF 268


>ref|XP_023751376.1| 4-coumarate--CoA ligase 1-like [Lactuca sativa]
 gb|PLY94799.1| hypothetical protein LSAT_2X103361 [Lactuca sativa]
          Length = 565

 Score =  204 bits (519), Expect(2) = 3e-66
 Identities = 98/133 (73%), Positives = 108/133 (81%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVIL  VK++G GYDLSSLRR+GSGAAPLSKE  + FR +FPWV LRPGYGLTESCG
Sbjct: 304 AVPPVILSLVKHDGGGYDLSSLRRVGSGAAPLSKELVDRFRARFPWVALRPGYGLTESCG 363

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           AAT FVSNEEAK   A S  L P F AKVVDFETG+ +PPYKEGELW+K PTVMK YLGN
Sbjct: 364 AATLFVSNEEAKTHLAGSGGLLPSFCAKVVDFETGVAMPPYKEGELWLKGPTVMKGYLGN 423

Query: 484 EAANAAAFDSEGW 522
           E A AA  +S+GW
Sbjct: 424 EEATAATINSDGW 436



 Score = 75.9 bits (185), Expect(2) = 3e-66
 Identities = 38/49 (77%), Positives = 41/49 (83%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLFSRTSGYTRV 149
           SV+TLLKWSVD   AN+DVFLCFIP+FHIYGLAFFGLGLF   SG T V
Sbjct: 234 SVMTLLKWSVDNMSANNDVFLCFIPMFHIYGLAFFGLGLF--CSGTTTV 280


>ref|XP_010090435.1| 4-coumarate--CoA ligase-like 5 [Morus notabilis]
 gb|EXB39450.1| 4-coumarate--CoA ligase-like 5 [Morus notabilis]
          Length = 572

 Score =  197 bits (500), Expect(2) = 3e-64
 Identities = 99/135 (73%), Positives = 106/135 (78%), Gaps = 2/135 (1%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGK--GYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTES 297
           AVPPVILG VKY GK  G DLSSLRR+GSGAAPLSKE  E FR++FPWVELRPGYGLTES
Sbjct: 309 AVPPVILGMVKYAGKFAGVDLSSLRRVGSGAAPLSKELVEEFRQRFPWVELRPGYGLTES 368

Query: 298 CGAATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYL 477
           CGAAT F SN++AK R  SS RL P F AK+VD ET   LPPYKEGELWIKSPTVMKEYL
Sbjct: 369 CGAATIFASNKDAKTRSGSSGRLIPGFCAKIVDVETRKSLPPYKEGELWIKSPTVMKEYL 428

Query: 478 GNEAANAAAFDSEGW 522
           G   A A    S+GW
Sbjct: 429 GKTEATAETIVSDGW 443



 Score = 76.3 bits (186), Expect(2) = 3e-64
 Identities = 33/40 (82%), Positives = 38/40 (95%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLF 122
           SV+TLLKWS+DV+ A +DVFLCFIP+FHIYGLAFFGLGLF
Sbjct: 239 SVITLLKWSMDVTSAQNDVFLCFIPLFHIYGLAFFGLGLF 278


>gb|KVI05000.1| AMP-binding, conserved site-containing protein [Cynara cardunculus
           var. scolymus]
          Length = 549

 Score =  203 bits (517), Expect(2) = 9e-64
 Identities = 96/133 (72%), Positives = 108/133 (81%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVILG VKY+G GYDLSSLR +GSGAAPLSK  A  FR KFPW++L+PGYGLTE CG
Sbjct: 302 AVPPVILGLVKYDGGGYDLSSLRSVGSGAAPLSKHLASRFRAKFPWIDLKPGYGLTEGCG 361

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           A  FFVS+EEAKAR A+S  L P FSAKVVDFETG+ LPPY EGELW+K P VMK YLGN
Sbjct: 362 AVAFFVSSEEAKARSAASGALLPTFSAKVVDFETGIALPPYSEGELWLKGPAVMKGYLGN 421

Query: 484 EAANAAAFDSEGW 522
           EAA +A   ++GW
Sbjct: 422 EAATSATIVADGW 434



 Score = 68.2 bits (165), Expect(2) = 9e-64
 Identities = 31/49 (63%), Positives = 40/49 (81%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLFSRTSGYTRV 149
           S++ LLKW+V+++ + DD+FLCFIP+FHIYGLAFFGLGL    SG T V
Sbjct: 232 SMMMLLKWAVEITASKDDIFLCFIPMFHIYGLAFFGLGLL--CSGTTTV 278


>ref|XP_002271586.3| PREDICTED: 4-coumarate--CoA ligase-like 5 [Vitis vinifera]
 emb|CAN70408.1| hypothetical protein VITISV_021990 [Vitis vinifera]
          Length = 569

 Score =  199 bits (505), Expect(2) = 3e-63
 Identities = 100/133 (75%), Positives = 109/133 (81%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVILG VK N    DLSSLRR+GSGAAPLSKE AEGFR+KFPWVELR GYGLTESCG
Sbjct: 309 AVPPVILGLVK-NSSSCDLSSLRRVGSGAAPLSKEVAEGFREKFPWVELRTGYGLTESCG 367

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           AAT+FV++E+AKAR  SS RL P F AKVVD ETGL LPP +EGELW+KSPTVMK YLGN
Sbjct: 368 AATYFVTDEQAKARAGSSGRLLPRFCAKVVDTETGLALPPNREGELWLKSPTVMKGYLGN 427

Query: 484 EAANAAAFDSEGW 522
             A AA   S+GW
Sbjct: 428 AEATAATITSDGW 440



 Score = 70.9 bits (172), Expect(2) = 3e-63
 Identities = 33/49 (67%), Positives = 41/49 (83%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLFSRTSGYTRV 149
           +++TLLKWSVD++ +N+DVFLCFIP+FHIYGLAFF LGL    SG T V
Sbjct: 239 AMMTLLKWSVDITSSNNDVFLCFIPMFHIYGLAFFALGLL--RSGTTTV 285


>ref|XP_019198798.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Ipomoea nil]
          Length = 599

 Score =  204 bits (520), Expect(2) = 4e-63
 Identities = 99/134 (73%), Positives = 111/134 (82%), Gaps = 1/134 (0%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKG-YDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESC 300
           AVPPVILG VKYN +G YDLSSLRR+GSGAAPLSKE A+GFRKKFPWVELRPGYGLTES 
Sbjct: 337 AVPPVILGLVKYNNRGGYDLSSLRRVGSGAAPLSKEVADGFRKKFPWVELRPGYGLTESS 396

Query: 301 GAATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLG 480
           GAAT FVS++EA ARPASS  L PC SAKVVD ETG  LPP K+GELW++ P +M EY+G
Sbjct: 397 GAATSFVSSQEANARPASSGVLFPCISAKVVDTETGEALPPLKQGELWLRGPNIMNEYIG 456

Query: 481 NEAANAAAFDSEGW 522
           N+ A A   DS+GW
Sbjct: 457 NQEATANTLDSDGW 470



 Score = 64.7 bits (156), Expect(2) = 4e-63
 Identities = 28/40 (70%), Positives = 34/40 (85%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLF 122
           +V+TLLKW V  + + DDVFLCFIP+FHIYGL+FF LGLF
Sbjct: 267 AVMTLLKWYVASTSSQDDVFLCFIPMFHIYGLSFFALGLF 306


>ref|XP_023762700.1| 4-coumarate--CoA ligase 1-like [Lactuca sativa]
          Length = 565

 Score =  202 bits (513), Expect(2) = 6e-63
 Identities = 97/133 (72%), Positives = 109/133 (81%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVIL  VKYNG GYDLSSLR +GSGAAPLSK  A  FR+KFPWV+L+PGYGLTESCG
Sbjct: 304 AVPPVILAMVKYNGGGYDLSSLRSVGSGAAPLSKHVATRFREKFPWVDLKPGYGLTESCG 363

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           AA FFVS+EEA AR A+S  L P FSAKVVDFE+G+  PPY EGE+W+K PTVMK YLGN
Sbjct: 364 AAAFFVSSEEAIARSAASGGLFPSFSAKVVDFESGIAQPPYSEGEIWLKGPTVMKGYLGN 423

Query: 484 EAANAAAFDSEGW 522
           EAA +A   S+GW
Sbjct: 424 EAATSATIVSDGW 436



 Score = 67.0 bits (162), Expect(2) = 6e-63
 Identities = 27/39 (69%), Positives = 33/39 (84%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGL 119
           S +TLLKW+ + + A DD+FLCFIP+FHIYGL FFGLGL
Sbjct: 234 STITLLKWAAETTAAKDDIFLCFIPMFHIYGLVFFGLGL 272


>gb|PLY86340.1| hypothetical protein LSAT_8X17560 [Lactuca sativa]
          Length = 553

 Score =  202 bits (513), Expect(2) = 6e-63
 Identities = 97/133 (72%), Positives = 109/133 (81%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPPVIL  VKYNG GYDLSSLR +GSGAAPLSK  A  FR+KFPWV+L+PGYGLTESCG
Sbjct: 292 AVPPVILAMVKYNGGGYDLSSLRSVGSGAAPLSKHVATRFREKFPWVDLKPGYGLTESCG 351

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           AA FFVS+EEA AR A+S  L P FSAKVVDFE+G+  PPY EGE+W+K PTVMK YLGN
Sbjct: 352 AAAFFVSSEEAIARSAASGGLFPSFSAKVVDFESGIAQPPYSEGEIWLKGPTVMKGYLGN 411

Query: 484 EAANAAAFDSEGW 522
           EAA +A   S+GW
Sbjct: 412 EAATSATIVSDGW 424



 Score = 67.0 bits (162), Expect(2) = 6e-63
 Identities = 27/39 (69%), Positives = 33/39 (84%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGL 119
           S +TLLKW+ + + A DD+FLCFIP+FHIYGL FFGLGL
Sbjct: 222 STITLLKWAAETTAAKDDIFLCFIPMFHIYGLVFFGLGL 260


>ref|XP_007219482.1| 4-coumarate--CoA ligase-like 5 [Prunus persica]
 gb|ONI21826.1| hypothetical protein PRUPE_2G091700 [Prunus persica]
          Length = 569

 Score =  197 bits (500), Expect(2) = 1e-62
 Identities = 94/134 (70%), Positives = 108/134 (80%), Gaps = 1/134 (0%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGY-DLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESC 300
           AVPPVILG VKY  K   DLSSLRR+ SGAAPLSKE  + FR++FPWV++RPGYGLTESC
Sbjct: 305 AVPPVILGLVKYASKAACDLSSLRRVSSGAAPLSKEVVDEFRERFPWVQMRPGYGLTESC 364

Query: 301 GAATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLG 480
           GAATFF+S+E+A+  PAS   L P F AK+VD ETG  LPPYKEGELW+KSPT+MKEYLG
Sbjct: 365 GAATFFISDEQARGHPASCGLLLPTFCAKIVDIETGEALPPYKEGELWMKSPTIMKEYLG 424

Query: 481 NEAANAAAFDSEGW 522
           N  A AA  DS+GW
Sbjct: 425 NVEATAATIDSDGW 438



 Score = 71.2 bits (173), Expect(2) = 1e-62
 Identities = 34/49 (69%), Positives = 40/49 (81%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLFSRTSGYTRV 149
           +V+T L+W+VD + A DDVFLCFIP+FHIYGLAFFGLGLF   SG T V
Sbjct: 235 AVMTTLRWTVDGTSAQDDVFLCFIPMFHIYGLAFFGLGLF--CSGITTV 281


>ref|XP_021818824.1| 4-coumarate--CoA ligase-like 5 isoform X1 [Prunus avium]
          Length = 596

 Score =  194 bits (493), Expect(2) = 3e-62
 Identities = 94/134 (70%), Positives = 107/134 (79%), Gaps = 1/134 (0%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGY-DLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESC 300
           AVPPVILG VKY  K   DLSSLRR+ SGAAPLSKE  + FR++FPWV++RPGYGLTESC
Sbjct: 305 AVPPVILGLVKYASKAACDLSSLRRVSSGAAPLSKEVVDEFRERFPWVQMRPGYGLTESC 364

Query: 301 GAATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLG 480
           GAATFFVS+E+A+   AS   L P F AK+VD ETG  LPPYKEGELW+KSPT+MKEYLG
Sbjct: 365 GAATFFVSDEQARGHSASCGLLLPSFCAKIVDVETGEALPPYKEGELWLKSPTIMKEYLG 424

Query: 481 NEAANAAAFDSEGW 522
           N  A AA  DS+GW
Sbjct: 425 NVEATAATIDSDGW 438



 Score = 72.4 bits (176), Expect(2) = 3e-62
 Identities = 34/49 (69%), Positives = 41/49 (83%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLFSRTSGYTRV 149
           +V+T+L+W+VD + A DDVFLCFIP+FHIYGLAFFGLGLF   SG T V
Sbjct: 235 AVMTMLRWTVDETSAQDDVFLCFIPMFHIYGLAFFGLGLF--CSGTTTV 281


>ref|XP_021818825.1| 4-coumarate--CoA ligase-like 5 isoform X2 [Prunus avium]
          Length = 569

 Score =  194 bits (493), Expect(2) = 3e-62
 Identities = 94/134 (70%), Positives = 107/134 (79%), Gaps = 1/134 (0%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGY-DLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESC 300
           AVPPVILG VKY  K   DLSSLRR+ SGAAPLSKE  + FR++FPWV++RPGYGLTESC
Sbjct: 305 AVPPVILGLVKYASKAACDLSSLRRVSSGAAPLSKEVVDEFRERFPWVQMRPGYGLTESC 364

Query: 301 GAATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLG 480
           GAATFFVS+E+A+   AS   L P F AK+VD ETG  LPPYKEGELW+KSPT+MKEYLG
Sbjct: 365 GAATFFVSDEQARGHSASCGLLLPSFCAKIVDVETGEALPPYKEGELWLKSPTIMKEYLG 424

Query: 481 NEAANAAAFDSEGW 522
           N  A AA  DS+GW
Sbjct: 425 NVEATAATIDSDGW 438



 Score = 72.4 bits (176), Expect(2) = 3e-62
 Identities = 34/49 (69%), Positives = 41/49 (83%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLFSRTSGYTRV 149
           +V+T+L+W+VD + A DDVFLCFIP+FHIYGLAFFGLGLF   SG T V
Sbjct: 235 AVMTMLRWTVDETSAQDDVFLCFIPMFHIYGLAFFGLGLF--CSGTTTV 281


>ref|XP_009350226.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Pyrus x bretschneideri]
 ref|XP_009350228.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Pyrus x bretschneideri]
          Length = 569

 Score =  195 bits (495), Expect(2) = 8e-62
 Identities = 95/134 (70%), Positives = 108/134 (80%), Gaps = 1/134 (0%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGK-GYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESC 300
           AVPPVILG VK+  K   DLSSLRR+GSGAAPLSKE  + FRK+FPWVE+RPGYGLTESC
Sbjct: 305 AVPPVILGLVKHASKTACDLSSLRRVGSGAAPLSKELIDEFRKRFPWVEMRPGYGLTESC 364

Query: 301 GAATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLG 480
           GAATFF+S+++AKA  AS   L P F AK+VD ETG  LPPYKEGELW+KSPT+MKEYLG
Sbjct: 365 GAATFFISDKQAKAHSASCGELLPGFLAKIVDVETGEALPPYKEGELWLKSPTIMKEYLG 424

Query: 481 NEAANAAAFDSEGW 522
           N  A  A  DS+GW
Sbjct: 425 NVEATLATIDSDGW 438



 Score = 70.1 bits (170), Expect(2) = 8e-62
 Identities = 28/40 (70%), Positives = 36/40 (90%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLF 122
           +++ +L+W+VD + A DDVFLCFIP+FHIYGLAFFGLGLF
Sbjct: 235 AIIAMLRWTVDKTSAQDDVFLCFIPMFHIYGLAFFGLGLF 274


>ref|XP_009361476.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Pyrus x bretschneideri]
          Length = 569

 Score =  195 bits (495), Expect(2) = 8e-62
 Identities = 95/134 (70%), Positives = 108/134 (80%), Gaps = 1/134 (0%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGK-GYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESC 300
           AVPPVILG VK+  K   DLSSLRR+GSGAAPLSKE  + FRK+FPWVE+RPGYGLTESC
Sbjct: 305 AVPPVILGLVKHASKTACDLSSLRRVGSGAAPLSKELIDEFRKRFPWVEMRPGYGLTESC 364

Query: 301 GAATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLG 480
           GAATFF+S+++AKA  AS   L P F AK+VD ETG  LPPYKEGELW+KSPT+MKEYLG
Sbjct: 365 GAATFFISDKQAKAHSASCGELLPGFLAKIVDVETGEALPPYKEGELWLKSPTIMKEYLG 424

Query: 481 NEAANAAAFDSEGW 522
           N  A  A  DS+GW
Sbjct: 425 NVEATLATIDSDGW 438



 Score = 70.1 bits (170), Expect(2) = 8e-62
 Identities = 28/40 (70%), Positives = 36/40 (90%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLF 122
           +++ +L+W+VD + A DDVFLCFIP+FHIYGLAFFGLGLF
Sbjct: 235 AIIAMLRWTVDKTSAQDDVFLCFIPMFHIYGLAFFGLGLF 274


>ref|XP_021993469.1| 4-coumarate--CoA ligase-like 5 [Helianthus annuus]
          Length = 562

 Score =  204 bits (519), Expect(2) = 8e-62
 Identities = 97/133 (72%), Positives = 109/133 (81%)
 Frame = +1

Query: 124 AVPPVILGFVKYNGKGYDLSSLRRMGSGAAPLSKETAEGFRKKFPWVELRPGYGLTESCG 303
           AVPP+IL  VK+N  GYDLSSLRR+GSGAAPLSKE  + FR KFPWV LRPGYGLTESCG
Sbjct: 301 AVPPIILSLVKHNKGGYDLSSLRRVGSGAAPLSKELVDRFRAKFPWVHLRPGYGLTESCG 360

Query: 304 AATFFVSNEEAKARPASSARLAPCFSAKVVDFETGLGLPPYKEGELWIKSPTVMKEYLGN 483
           AAT F+++EEAKA  A S +L P F AKVVD+ETG  +PPY EGELW+KSPTVMK YLGN
Sbjct: 361 AATLFLTHEEAKAHHAGSGKLLPTFCAKVVDYETGAAMPPYGEGELWLKSPTVMKGYLGN 420

Query: 484 EAANAAAFDSEGW 522
           EAA AA  DS+GW
Sbjct: 421 EAATAATIDSDGW 433



 Score = 60.8 bits (146), Expect(2) = 8e-62
 Identities = 31/49 (63%), Positives = 35/49 (71%)
 Frame = +3

Query: 3   SVVTLLKWSVDVSKANDDVFLCFIPIFHIYGLAFFGLGLFSRTSGYTRV 149
           S +TLLKWS     A+ DVFLCF+P+FHIYGLAFF LGL   T G T V
Sbjct: 231 SCMTLLKWSTYKMSADQDVFLCFLPMFHIYGLAFFTLGLL--TFGATTV 277