BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00016335
         (1074 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_012851911.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   196   5e-75
gb|EYU25065.1| hypothetical protein MIMGU_mgv1a019909mg, partial...   196   5e-75
ref|XP_012852332.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   195   4e-74
gb|EYU25066.1| hypothetical protein MIMGU_mgv1a021633mg, partial...   195   4e-74
ref|XP_012837735.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   192   7e-73
gb|EYU33145.1| hypothetical protein MIMGU_mgv1a024173mg, partial...   188   4e-72
ref|XP_012842397.1| PREDICTED: cytochrome P450 76AD1-like [Eryth...   166   2e-65
gb|PIM98211.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   165   5e-63
gb|PIM98731.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   165   5e-63
gb|PIM98733.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   165   2e-62
gb|PIN05630.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   167   5e-61
ref|XP_012846401.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   165   8e-61
ref|XP_011083904.2| probable (S)-N-methylcoclaurine 3'-hydroxyla...   162   8e-61
gb|EYU45167.1| hypothetical protein MIMGU_mgv1a024523mg [Erythra...   162   3e-59
ref|XP_012846393.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   162   3e-59
ref|XP_011084765.1| probable (S)-N-methylcoclaurine 3'-hydroxyla...   166   3e-58
gb|PIN02757.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   168   1e-57
gb|PIN00451.1| hypothetical protein CDL12_27045 [Handroanthus im...   157   1e-54
ref|XP_019223612.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   137   3e-49
ref|XP_010112571.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   137   4e-49

>ref|XP_012851911.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
           [Erythranthe guttata]
          Length = 604

 Score =  196 bits (497), Expect(2) = 5e-75
 Identities = 93/124 (75%), Positives = 109/124 (87%), Gaps = 2/124 (1%)
 Frame = +3

Query: 459 AGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGD--TLINESILTDSQYFQACIKE 632
           AG+D+S  T VWLMVELI+NPHIL +VRDEIA AFEGD  TLI+ESIL+DS YFQACIKE
Sbjct: 404 AGMDTSGMTSVWLMVELIKNPHILRRVRDEIASAFEGDDPTLIDESILSDSHYFQACIKE 463

Query: 633 TLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLD 812
           TLR HIP PFL+PH AIE CK+ +Y+IPKD++VLVNAWAIS++P  WKDATSFNPD+FL+
Sbjct: 464 TLRLHIPGPFLMPHCAIEACKIDDYIIPKDSVVLVNAWAISMDPDTWKDATSFNPDRFLE 523

Query: 813 SKID 824
           SKID
Sbjct: 524 SKID 527



 Score =  115 bits (288), Expect(2) = 5e-75
 Identities = 53/76 (69%), Positives = 62/76 (81%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            FRG HFE+IPFS+GRR C G N+A KNIQIVVASLVHYFDW+LP G+DPT+I+   KFG 
Sbjct: 528  FRGNHFEYIPFSSGRRMCPGINLALKNIQIVVASLVHYFDWNLPNGMDPTQIDMADKFGT 587

Query: 1004 VLKKEKQLCLTPSLRQ 1051
            VLKKE  L L PSL++
Sbjct: 588  VLKKETPLLLIPSLKK 603



 Score = 92.8 bits (229), Expect = 7e-17
 Identities = 42/56 (75%), Positives = 46/56 (82%)
 Frame = +2

Query: 830 GTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKF 997
           G HFEFIPF AGRR C G N+A KNIQIVVASLVHYFDWSLP  +DPT+I+T  KF
Sbjct: 47  GNHFEFIPFRAGRRMCPGFNLALKNIQIVVASLVHYFDWSLPDTMDPTRIDTAEKF 102


>gb|EYU25065.1| hypothetical protein MIMGU_mgv1a019909mg, partial [Erythranthe
           guttata]
          Length = 482

 Score =  196 bits (497), Expect(2) = 5e-75
 Identities = 93/124 (75%), Positives = 109/124 (87%), Gaps = 2/124 (1%)
 Frame = +3

Query: 459 AGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGD--TLINESILTDSQYFQACIKE 632
           AG+D+S  T VWLMVELI+NPHIL +VRDEIA AFEGD  TLI+ESIL+DS YFQACIKE
Sbjct: 282 AGMDTSGMTSVWLMVELIKNPHILRRVRDEIASAFEGDDPTLIDESILSDSHYFQACIKE 341

Query: 633 TLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLD 812
           TLR HIP PFL+PH AIE CK+ +Y+IPKD++VLVNAWAIS++P  WKDATSFNPD+FL+
Sbjct: 342 TLRLHIPGPFLMPHCAIEACKIDDYIIPKDSVVLVNAWAISMDPDTWKDATSFNPDRFLE 401

Query: 813 SKID 824
           SKID
Sbjct: 402 SKID 405



 Score =  115 bits (288), Expect(2) = 5e-75
 Identities = 53/76 (69%), Positives = 62/76 (81%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            FRG HFE+IPFS+GRR C G N+A KNIQIVVASLVHYFDW+LP G+DPT+I+   KFG 
Sbjct: 406  FRGNHFEYIPFSSGRRMCPGINLALKNIQIVVASLVHYFDWNLPNGMDPTQIDMADKFGT 465

Query: 1004 VLKKEKQLCLTPSLRQ 1051
            VLKKE  L L PSL++
Sbjct: 466  VLKKETPLLLIPSLKK 481


>ref|XP_012852332.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Erythranthe guttata]
          Length = 501

 Score =  195 bits (496), Expect(2) = 4e-74
 Identities = 93/124 (75%), Positives = 109/124 (87%), Gaps = 2/124 (1%)
 Frame = +3

Query: 459 AGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGD--TLINESILTDSQYFQACIKE 632
           AG+D+S  T VWLMVELI+NPHIL +VRDEIA AFEGD  TLI+ESIL+DS YFQACIKE
Sbjct: 301 AGMDTSGITSVWLMVELIKNPHILRRVRDEIASAFEGDDRTLIDESILSDSHYFQACIKE 360

Query: 633 TLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLD 812
           TLR HIP P L+P RAIETCK+ +YVIPKD+++LVNAWAIS++P  WKDATSFNPD+FL+
Sbjct: 361 TLRLHIPGPLLMPRRAIETCKIDDYVIPKDSVILVNAWAISMDPDTWKDATSFNPDRFLE 420

Query: 813 SKID 824
           SKID
Sbjct: 421 SKID 424



 Score =  113 bits (282), Expect(2) = 4e-74
 Identities = 52/76 (68%), Positives = 61/76 (80%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            FRG HFE+IPF +GRR C G N+A K+IQIVVASLVHYFDWSLP G+DPT+I+   KFG 
Sbjct: 425  FRGNHFEYIPFGSGRRMCPGFNLALKDIQIVVASLVHYFDWSLPNGMDPTQIDMADKFGT 484

Query: 1004 VLKKEKQLCLTPSLRQ 1051
            VLKKE  L L PSL++
Sbjct: 485  VLKKETPLLLIPSLKK 500


>gb|EYU25066.1| hypothetical protein MIMGU_mgv1a021633mg, partial [Erythranthe
           guttata]
          Length = 471

 Score =  195 bits (496), Expect(2) = 4e-74
 Identities = 93/124 (75%), Positives = 109/124 (87%), Gaps = 2/124 (1%)
 Frame = +3

Query: 459 AGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGD--TLINESILTDSQYFQACIKE 632
           AG+D+S  T VWLMVELI+NPHIL +VRDEIA AFEGD  TLI+ESIL+DS YFQACIKE
Sbjct: 271 AGMDTSGITSVWLMVELIKNPHILRRVRDEIASAFEGDDRTLIDESILSDSHYFQACIKE 330

Query: 633 TLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLD 812
           TLR HIP P L+P RAIETCK+ +YVIPKD+++LVNAWAIS++P  WKDATSFNPD+FL+
Sbjct: 331 TLRLHIPGPLLMPRRAIETCKIDDYVIPKDSVILVNAWAISMDPDTWKDATSFNPDRFLE 390

Query: 813 SKID 824
           SKID
Sbjct: 391 SKID 394



 Score =  113 bits (282), Expect(2) = 4e-74
 Identities = 52/76 (68%), Positives = 61/76 (80%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            FRG HFE+IPF +GRR C G N+A K+IQIVVASLVHYFDWSLP G+DPT+I+   KFG 
Sbjct: 395  FRGNHFEYIPFGSGRRMCPGFNLALKDIQIVVASLVHYFDWSLPNGMDPTQIDMADKFGT 454

Query: 1004 VLKKEKQLCLTPSLRQ 1051
            VLKKE  L L PSL++
Sbjct: 455  VLKKETPLLLIPSLKK 470


>ref|XP_012837735.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Erythranthe guttata]
 gb|EYU37231.1| hypothetical protein MIMGU_mgv1a005037mg [Erythranthe guttata]
          Length = 499

 Score =  192 bits (488), Expect(2) = 7e-73
 Identities = 97/143 (67%), Positives = 115/143 (80%), Gaps = 2/143 (1%)
 Frame = +3

Query: 402 SKSDSDIYIYIYMLSLGAIAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLI 581
           S +D  IY+    L    IAG DSSS TLVWLM ELI+NP IL KV +E+AKAFEGD LI
Sbjct: 279 SLTDDQIYVVFTEL---LIAGTDSSSITLVWLMAELIKNPDILRKVVEEVAKAFEGDLLI 335

Query: 582 NESILTDSQYFQACIKETLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVE 761
           +E+ILT+S+Y QACIKETLRFH+P PFLVPHRAIE CK+ +YVIPKD++VLVN W IS++
Sbjct: 336 DEAILTESKYLQACIKETLRFHMPGPFLVPHRAIEKCKIHDYVIPKDSVVLVNTWDISLD 395

Query: 762 PSNWKDATSFNPDKFL--DSKID 824
           P  WKDA+SFNPD+FL  DSKID
Sbjct: 396 PKYWKDASSFNPDRFLTGDSKID 418



 Score =  112 bits (279), Expect(2) = 7e-73
 Identities = 52/75 (69%), Positives = 61/75 (81%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            FRG HFE+ PF AGRR C G N+AFK+IQIVVASLVHYFDWSLP G+DPT+I+T S FG 
Sbjct: 419  FRGAHFEYTPFGAGRRMCPGFNLAFKSIQIVVASLVHYFDWSLPNGMDPTRIDTDSVFGT 478

Query: 1004 VLKKEKQLCLTPSLR 1048
            VLKK++ L L P+ R
Sbjct: 479  VLKKKQPLLLIPTPR 493


>gb|EYU33145.1| hypothetical protein MIMGU_mgv1a024173mg, partial [Erythranthe
           guttata]
          Length = 218

 Score =  188 bits (477), Expect(2) = 4e-72
 Identities = 90/125 (72%), Positives = 108/125 (86%), Gaps = 2/125 (1%)
 Frame = +3

Query: 456 IAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESILTDSQYFQACIKET 635
           IAG DSSS TLVWLM ELI+NP IL +V +E+AKAFEGD LI+E++LT+S+Y QACIKET
Sbjct: 13  IAGTDSSSITLVWLMAELIKNPDILRRVVEEVAKAFEGDMLIDEAVLTESKYLQACIKET 72

Query: 636 LRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFL-- 809
           LRFH+P PFLVPHRAIE CK+ +YVIPKD++VLVN W IS++P  WKDA+SFNPD+FL  
Sbjct: 73  LRFHMPGPFLVPHRAIEKCKIHDYVIPKDSVVLVNTWDISLDPEYWKDASSFNPDRFLTG 132

Query: 810 DSKID 824
           DSKID
Sbjct: 133 DSKID 137



 Score =  113 bits (283), Expect(2) = 4e-72
 Identities = 53/75 (70%), Positives = 61/75 (81%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            FRG HFE+ PF AGRR C G N+AFK+IQIVVASLVHYFDWSLP G+DPT+I+T S FG 
Sbjct: 138  FRGAHFEYTPFGAGRRMCPGFNLAFKSIQIVVASLVHYFDWSLPNGMDPTRIDTDSVFGT 197

Query: 1004 VLKKEKQLCLTPSLR 1048
            VLKK+K L L P+ R
Sbjct: 198  VLKKKKPLLLIPTPR 212


>ref|XP_012842397.1| PREDICTED: cytochrome P450 76AD1-like [Erythranthe guttata]
          Length = 192

 Score =  166 bits (419), Expect(2) = 2e-65
 Identities = 78/111 (70%), Positives = 96/111 (86%), Gaps = 2/111 (1%)
 Frame = +3

Query: 498 MVELIRNPHILHKVRDEIAKAFEGDTLINESILTDSQYFQACIKETLRFHIPVPFLVPHR 677
           M ELI+NP IL +V +E+AKAFEGD LI+E++LT+S+Y QACIKETLRFH+P PFLVPHR
Sbjct: 1   MAELIKNPDILRRVVEEVAKAFEGDMLIDEAVLTESKYLQACIKETLRFHMPGPFLVPHR 60

Query: 678 AIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFL--DSKID 824
           AIE CK+ +YVIPKD++VLVN W IS++P  WKDA+SFNPD+FL  DSKID
Sbjct: 61  AIEKCKIHDYVIPKDSVVLVNTWDISLDPEYWKDASSFNPDRFLTGDSKID 111



 Score =  113 bits (283), Expect(2) = 2e-65
 Identities = 53/75 (70%), Positives = 61/75 (81%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            FRG HFE+ PF AGRR C G N+AFK+IQIVVASLVHYFDWSLP G+DPT+I+T S FG 
Sbjct: 112  FRGAHFEYTPFGAGRRMCPGFNLAFKSIQIVVASLVHYFDWSLPNGMDPTRIDTDSVFGT 171

Query: 1004 VLKKEKQLCLTPSLR 1048
            VLKK+K L L P+ R
Sbjct: 172  VLKKKKPLLLIPTPR 186


>gb|PIM98211.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 493

 Score =  165 bits (417), Expect(2) = 5e-63
 Identities = 76/122 (62%), Positives = 97/122 (79%)
 Frame = +3

Query: 456 IAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESILTDSQYFQACIKET 635
           I   DS++ T VWLMVEL+RN  +L +VR+E+A+  EG+ L NES+L    YFQACIKET
Sbjct: 291 IGATDSTTITTVWLMVELLRNQEMLSRVRNELAQTIEGNEL-NESLLPQCLYFQACIKET 349

Query: 636 LRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLDS 815
           LR H+P P LVPHRA ETCK+ NY+IPKD+MVLVNAW I+ +P+ W+DATSF P++F+DS
Sbjct: 350 LRLHVPAPLLVPHRATETCKVNNYIIPKDSMVLVNAWGIARDPNYWEDATSFKPERFMDS 409

Query: 816 KI 821
           KI
Sbjct: 410 KI 411



 Score =  106 bits (264), Expect(2) = 5e-63
 Identities = 48/76 (63%), Positives = 59/76 (77%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            +RGTHFE++PF  G R C GS+VAFKNIQ++V SL++YFDWSLP G DPT I+T  K+  
Sbjct: 413  YRGTHFEYLPFGTGLRMCPGSSVAFKNIQMLVGSLLYYFDWSLPNGKDPTTIDTKEKYVT 472

Query: 1004 VLKKEKQLCLTPSLRQ 1051
             LKKEK L LTP LR+
Sbjct: 473  TLKKEKPLLLTPKLRE 488


>gb|PIM98731.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 357

 Score =  165 bits (417), Expect(2) = 5e-63
 Identities = 76/122 (62%), Positives = 97/122 (79%)
 Frame = +3

Query: 456 IAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESILTDSQYFQACIKET 635
           I   DS++ T VWLMVEL+RN  +L +VR+E+A+  EG+ L NES+L    YFQACIKET
Sbjct: 155 IGATDSTTITTVWLMVELLRNQEMLSRVRNELAQTIEGNEL-NESLLPQCLYFQACIKET 213

Query: 636 LRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLDS 815
           LR H+P P LVPHRA ETCK+ NY+IPKD+MVLVNAW I+ +P+ W+DATSF P++F+DS
Sbjct: 214 LRLHVPAPLLVPHRATETCKVNNYIIPKDSMVLVNAWGIARDPNYWEDATSFKPERFMDS 273

Query: 816 KI 821
           KI
Sbjct: 274 KI 275



 Score =  106 bits (264), Expect(2) = 5e-63
 Identities = 48/76 (63%), Positives = 59/76 (77%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            +RGTHFE++PF  G R C GS+VAFKNIQ++V SL++YFDWSLP G DPT I+T  K+  
Sbjct: 277  YRGTHFEYLPFGTGLRMCPGSSVAFKNIQMLVGSLLYYFDWSLPNGKDPTTIDTKEKYVT 336

Query: 1004 VLKKEKQLCLTPSLRQ 1051
             LKKEK L LTP LR+
Sbjct: 337  TLKKEKPLLLTPKLRE 352


>gb|PIM98733.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 493

 Score =  165 bits (417), Expect(2) = 2e-62
 Identities = 76/122 (62%), Positives = 96/122 (78%)
 Frame = +3

Query: 456 IAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESILTDSQYFQACIKET 635
           I   DS++ T VWLM+EL++N  IL KVR EIA+  EGD L +ES+L+   YFQACIKET
Sbjct: 291 IGATDSTTITTVWLMIELLKNQEILSKVRAEIAQTVEGDEL-SESLLSQCHYFQACIKET 349

Query: 636 LRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLDS 815
           LR H+P P LVPHRA+ETC + NY+IPKD+MVLVNAW I+ +P  W+DATSF P++FLDS
Sbjct: 350 LRLHVPAPLLVPHRAVETCMVNNYIIPKDSMVLVNAWGIARDPKYWEDATSFKPERFLDS 409

Query: 816 KI 821
           +I
Sbjct: 410 RI 411



 Score =  104 bits (260), Expect(2) = 2e-62
 Identities = 47/75 (62%), Positives = 58/75 (77%)
 Frame = +2

Query: 827  RGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGIV 1006
            RGTHFE++PF  G R C GS+VAFKNIQ++V SL+HYFDW+LP G DPT I+T  K+   
Sbjct: 414  RGTHFEYLPFGTGLRMCPGSSVAFKNIQLLVGSLLHYFDWNLPNGGDPTTIDTNEKYVTT 473

Query: 1007 LKKEKQLCLTPSLRQ 1051
            LKKEK L L P+LR+
Sbjct: 474  LKKEKPLLLIPTLRE 488


>gb|PIN05630.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 493

 Score =  167 bits (422), Expect(2) = 5e-61
 Identities = 79/122 (64%), Positives = 97/122 (79%)
 Frame = +3

Query: 456 IAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESILTDSQYFQACIKET 635
           I   DS++ T VWLMVEL++N  +L KVRDEIA+  EGD L +ES+L+   YFQACIKET
Sbjct: 291 IGATDSTTITTVWLMVELLKNQEMLSKVRDEIAQTVEGDEL-SESLLSQCHYFQACIKET 349

Query: 636 LRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLDS 815
           LR H+P P LVPHRAIETCK+ NYVIPKD+MVLVNAW I+ +P  W+DATSF P++FL S
Sbjct: 350 LRLHLPAPLLVPHRAIETCKVNNYVIPKDSMVLVNAWGIARDPKYWEDATSFKPERFLAS 409

Query: 816 KI 821
           +I
Sbjct: 410 RI 411



 Score = 97.8 bits (242), Expect(2) = 5e-61
 Identities = 45/75 (60%), Positives = 54/75 (72%)
 Frame = +2

Query: 827  RGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGIV 1006
            RG HFE++PF  G R C GS+VAFKNIQ++V SL+HYFDWSLP G D   I+T  K+   
Sbjct: 414  RGAHFEYLPFGTGLRMCPGSSVAFKNIQLLVGSLLHYFDWSLPNGKDLITIDTNEKYVTT 473

Query: 1007 LKKEKQLCLTPSLRQ 1051
            LKKEK L L P LR+
Sbjct: 474  LKKEKPLLLIPRLRE 488


>ref|XP_012846401.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Erythranthe guttata]
 gb|EYU45168.1| hypothetical protein MIMGU_mgv1a023853mg [Erythranthe guttata]
          Length = 493

 Score =  165 bits (418), Expect(2) = 8e-61
 Identities = 83/139 (59%), Positives = 107/139 (76%), Gaps = 1/139 (0%)
 Frame = +3

Query: 408 SDSDIYIYIYMLSLGAIAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINE 587
           S+  I I +  L +GA    DS++ T VWL+VELI+NP +L KVR+EIA+A EGD L NE
Sbjct: 282 SEDQIAISLMELLIGAT---DSTTITTVWLIVELIKNPEMLSKVREEIAQAVEGDEL-NE 337

Query: 588 SILTDS-QYFQACIKETLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEP 764
           S+L+++  YFQACIKETLR H+P P LVPHRAIETCK+ NY IPK++MVLVN+W I+ +P
Sbjct: 338 SLLSNNCNYFQACIKETLRLHVPAPLLVPHRAIETCKVNNYTIPKNSMVLVNSWGIARDP 397

Query: 765 SNWKDATSFNPDKFLDSKI 821
             W+DAT F P++FLDS I
Sbjct: 398 KYWEDATIFKPERFLDSNI 416



 Score = 98.6 bits (244), Expect(2) = 8e-61
 Identities = 45/76 (59%), Positives = 54/76 (71%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            +RGTHFE++PF  G R C GS VAFKNIQ++V SL+HYFDWSLP G DP  I+    +  
Sbjct: 418  YRGTHFEYLPFGTGLRMCPGSAVAFKNIQMLVGSLLHYFDWSLPNGDDPISIDMSETYLT 477

Query: 1004 VLKKEKQLCLTPSLRQ 1051
             LKKEK L L P LR+
Sbjct: 478  TLKKEKPLLLVPKLRE 493


>ref|XP_011083904.2| probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Sesamum
           indicum]
          Length = 423

 Score =  162 bits (411), Expect(2) = 8e-61
 Identities = 77/122 (63%), Positives = 93/122 (76%)
 Frame = +3

Query: 456 IAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESILTDSQYFQACIKET 635
           I   DS++ T VWLMVELIRN  +L +VRDEIA+   GD   +ES+L+   YFQACIKET
Sbjct: 224 IGATDSTTITTVWLMVELIRNQEMLSRVRDEIAQTVTGDKP-SESLLSQCHYFQACIKET 282

Query: 636 LRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLDS 815
           LR H+P P LVPHRA ETCK+ NYVIPKD+M+LVNAW I+ +P  W DAT F PD+FLD+
Sbjct: 283 LRLHVPAPLLVPHRATETCKVNNYVIPKDSMILVNAWGIARDPDYWNDATVFKPDRFLDT 342

Query: 816 KI 821
           KI
Sbjct: 343 KI 344



 Score =  101 bits (251), Expect(2) = 8e-61
 Identities = 47/75 (62%), Positives = 55/75 (73%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            FRGTHFE++PF  G R C GS VAFKNIQ++V SL+HYFDWSLPGG+  T I+   K+  
Sbjct: 346  FRGTHFEYLPFGTGLRMCPGSTVAFKNIQMLVGSLLHYFDWSLPGGVALTAIDMNEKYVT 405

Query: 1004 VLKKEKQLCLTPSLR 1048
             LKKEK L L P LR
Sbjct: 406  TLKKEKPLLLIPKLR 420


>gb|EYU45167.1| hypothetical protein MIMGU_mgv1a024523mg [Erythranthe guttata]
          Length = 482

 Score =  162 bits (409), Expect(2) = 3e-59
 Identities = 77/123 (62%), Positives = 99/123 (80%), Gaps = 1/123 (0%)
 Frame = +3

Query: 456 IAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESILTDS-QYFQACIKE 632
           I   D+++ T VWLMVELI+NP +L KVRDEI++AF+GD L NES+L+++  YFQACIKE
Sbjct: 279 IGATDTTTITSVWLMVELIKNPEMLSKVRDEISQAFKGDEL-NESLLSNNCNYFQACIKE 337

Query: 633 TLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLD 812
           TLR H+P P LVPHRA E CK+ NY IPK++MVLVNAW I+ +PS W+DA  F P++FL+
Sbjct: 338 TLRLHVPAPLLVPHRATEACKVNNYTIPKNSMVLVNAWGIARDPSYWEDAPIFKPERFLE 397

Query: 813 SKI 821
           SKI
Sbjct: 398 SKI 400



 Score = 97.1 bits (240), Expect(2) = 3e-59
 Identities = 45/75 (60%), Positives = 54/75 (72%)
 Frame = +2

Query: 827  RGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGIV 1006
            RGTHFE++PF  G R C GS VAFKNIQI+V SL+HYFDWSLP G D + I+T  K+   
Sbjct: 403  RGTHFEYLPFGTGLRMCPGSTVAFKNIQILVGSLLHYFDWSLPNGEDISSIDTNEKYITT 462

Query: 1007 LKKEKQLCLTPSLRQ 1051
            LKKEK L L   +R+
Sbjct: 463  LKKEKPLLLVSRIRE 477


>ref|XP_012846393.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Erythranthe guttata]
          Length = 426

 Score =  162 bits (409), Expect(2) = 3e-59
 Identities = 77/123 (62%), Positives = 99/123 (80%), Gaps = 1/123 (0%)
 Frame = +3

Query: 456 IAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESILTDS-QYFQACIKE 632
           I   D+++ T VWLMVELI+NP +L KVRDEI++AF+GD L NES+L+++  YFQACIKE
Sbjct: 223 IGATDTTTITSVWLMVELIKNPEMLSKVRDEISQAFKGDEL-NESLLSNNCNYFQACIKE 281

Query: 633 TLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLD 812
           TLR H+P P LVPHRA E CK+ NY IPK++MVLVNAW I+ +PS W+DA  F P++FL+
Sbjct: 282 TLRLHVPAPLLVPHRATEACKVNNYTIPKNSMVLVNAWGIARDPSYWEDAPIFKPERFLE 341

Query: 813 SKI 821
           SKI
Sbjct: 342 SKI 344



 Score = 97.1 bits (240), Expect(2) = 3e-59
 Identities = 45/75 (60%), Positives = 54/75 (72%)
 Frame = +2

Query: 827  RGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGIV 1006
            RGTHFE++PF  G R C GS VAFKNIQI+V SL+HYFDWSLP G D + I+T  K+   
Sbjct: 347  RGTHFEYLPFGTGLRMCPGSTVAFKNIQILVGSLLHYFDWSLPNGEDISSIDTNEKYITT 406

Query: 1007 LKKEKQLCLTPSLRQ 1051
            LKKEK L L   +R+
Sbjct: 407  LKKEKPLLLVSRIRE 421


>ref|XP_011084765.1| probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Sesamum
           indicum]
          Length = 498

 Score =  166 bits (419), Expect(2) = 3e-58
 Identities = 80/137 (58%), Positives = 102/137 (74%), Gaps = 1/137 (0%)
 Frame = +3

Query: 417 DIYIYIYMLSLGAIAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAF-EGDTLINESI 593
           D+  ++ M  L  IAG DS   T +WLMVEL+RN  IL +VR EI +A     T+IN+SI
Sbjct: 283 DLICFVLMELL--IAGGDSGVVTTIWLMVELMRNQDILSRVRQEIVEAMGTKTTVINDSI 340

Query: 594 LTDSQYFQACIKETLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNW 773
           L+  +YFQAC+KETLR HIP PFLVPH+A+E C + +Y IPKD++VLVNAW I ++P NW
Sbjct: 341 LSTCEYFQACVKETLRLHIPAPFLVPHQALENCVLNDYTIPKDSIVLVNAWTIQLDPGNW 400

Query: 774 KDATSFNPDKFLDSKID 824
           KDATSF PD+FL SK+D
Sbjct: 401 KDATSFKPDRFLGSKVD 417



 Score = 89.7 bits (221), Expect(2) = 3e-58
 Identities = 40/80 (50%), Positives = 55/80 (68%), Gaps = 1/80 (1%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            FRG HFEF+PF +GRR C G  + F+N+Q+VVA L+HYFDWSLP G +P  ++T      
Sbjct: 418  FRGNHFEFVPFGSGRRMCPGFQMGFRNVQLVVACLIHYFDWSLPDGENPENLDTADVLHA 477

Query: 1004 -VLKKEKQLCLTPSLRQ*YI 1060
              L++EK L + P LR+ Y+
Sbjct: 478  GGLRREKPLFMIPRLREKYV 497


>gb|PIN02757.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 490

 Score =  168 bits (426), Expect(2) = 1e-57
 Identities = 75/123 (60%), Positives = 97/123 (78%)
 Frame = +3

Query: 456 IAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESILTDSQYFQACIKET 635
           +AG+D+++ T+VWLM ELI+NP I HKVR EI      +  +NES+L++ QYFQACIKET
Sbjct: 291 VAGMDTTTITIVWLMAELIKNPEIFHKVRKEITDQAIQNNTLNESLLSECQYFQACIKET 350

Query: 636 LRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLDS 815
           LR HIP PF VPHRA ETC++ NY +PKD++VLVN WAI V+P+NWKD T F P++FL+S
Sbjct: 351 LRLHIPGPFGVPHRATETCQVNNYKVPKDSIVLVNLWAIQVDPNNWKDGTKFKPERFLNS 410

Query: 816 KID 824
            ID
Sbjct: 411 TID 413



 Score = 85.1 bits (209), Expect(2) = 1e-57
 Identities = 39/75 (52%), Positives = 53/75 (70%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            F+G   EFIPF AG R C GSNVA + +Q++VASLV+YFDW+LP G + ++++   +FG 
Sbjct: 414  FKGATSEFIPFGAGSRKCPGSNVALRVVQLIVASLVYYFDWNLPNGKNWSELDLTDRFGT 473

Query: 1004 VLKKEKQLCLTPSLR 1048
             LKK+K L L P  R
Sbjct: 474  TLKKDKPLYLIPKQR 488


>gb|PIN00451.1| hypothetical protein CDL12_27045 [Handroanthus impetiginosus]
          Length = 197

 Score =  157 bits (396), Expect(2) = 1e-54
 Identities = 71/120 (59%), Positives = 92/120 (76%)
 Frame = +3

Query: 465 IDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESILTDSQYFQACIKETLRF 644
           +D+++ T+VWLM ELI+NP I HKVR EI      +  +NES+L++ QY QACIKETLR 
Sbjct: 1   MDTTTITIVWLMAELIKNPEIFHKVRKEITDQAIQNNTLNESLLSECQYSQACIKETLRL 60

Query: 645 HIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNWKDATSFNPDKFLDSKID 824
           HIP PF VPHRA ETC++ N  +PKD++VLVN WAI V+P+NWKD T F P++FL+S ID
Sbjct: 61  HIPGPFGVPHRATETCQVNNCKVPKDSIVLVNLWAIQVDPNNWKDGTKFKPERFLNSTID 120



 Score = 86.3 bits (212), Expect(2) = 1e-54
 Identities = 40/75 (53%), Positives = 53/75 (70%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            F+G   EFIPF AG R C GSNVA + +Q++VASLV+YFDWSLP G + ++++   +FG 
Sbjct: 121  FKGATSEFIPFGAGSRKCPGSNVALRVVQLIVASLVYYFDWSLPNGKNWSELDMTDRFGT 180

Query: 1004 VLKKEKQLCLTPSLR 1048
             LKK+K L L P  R
Sbjct: 181  TLKKDKPLYLIPKQR 195


>ref|XP_019223612.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Nicotiana attenuata]
 gb|OIT33933.1| cytochrome p450 76c2 [Nicotiana attenuata]
          Length = 489

 Score =  137 bits (346), Expect(2) = 3e-49
 Identities = 63/137 (45%), Positives = 92/137 (67%)
 Frame = +3

Query: 414 SDIYIYIYMLSLGAIAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLINESI 593
           SD+ I I ++ L A AG D++++T+ W M EL+RN  ++HK++ E+      +  I ES 
Sbjct: 281 SDLQINIILMELIA-AGTDTTTSTIEWAMAELLRNKEVMHKLQAELRSKIGSNANIRESN 339

Query: 594 LTDSQYFQACIKETLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVEPSNW 773
           +++  Y  AC+KETLR H P PFL+P RA ETCK+ NY IPK++ + VN WAI  +   W
Sbjct: 340 ISELPYLAACVKETLRIHPPTPFLIPRRAPETCKLMNYTIPKNSRLFVNVWAIGRDSKTW 399

Query: 774 KDATSFNPDKFLDSKID 824
           +DA SF P++FLDS +D
Sbjct: 400 EDALSFRPERFLDSSVD 416



 Score = 87.8 bits (216), Expect(2) = 3e-49
 Identities = 37/72 (51%), Positives = 52/72 (72%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            FRG  FEFIPF AGRRTC G   A + + +++ASL+H+F+WS+P G DP +++   KFG+
Sbjct: 417  FRGQDFEFIPFGAGRRTCPGLPFARQEVHLILASLIHHFEWSIPNGEDPMQLDMEEKFGV 476

Query: 1004 VLKKEKQLCLTP 1039
             L+KEK L + P
Sbjct: 477  TLQKEKPLLVVP 488


>ref|XP_010112571.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Morus notabilis]
 gb|EXC34118.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Morus notabilis]
          Length = 499

 Score =  137 bits (346), Expect(2) = 4e-49
 Identities = 63/141 (44%), Positives = 94/141 (66%)
 Frame = +3

Query: 402 SKSDSDIYIYIYMLSLGAIAGIDSSSTTLVWLMVELIRNPHILHKVRDEIAKAFEGDTLI 581
           S +D   ++ I +++    AG D+SS+T+ W + ELI+NP  +  V+ E+A+      ++
Sbjct: 287 SSNDQINFLLIELIT----AGTDTSSSTIEWTLTELIKNPKCMKIVKAELAREIVDQDMV 342

Query: 582 NESILTDSQYFQACIKETLRFHIPVPFLVPHRAIETCKMGNYVIPKDNMVLVNAWAISVE 761
            ES L    Y QAC+KETLR H P PFL+PHRA+E+C++ NY IPKD+ +LVN WAI  +
Sbjct: 343 RESHLPKLPYLQACVKETLRLHPPAPFLLPHRAVESCQVLNYTIPKDSQLLVNVWAIGRD 402

Query: 762 PSNWKDATSFNPDKFLDSKID 824
           P  WKD  +F P++FL+S +D
Sbjct: 403 PMYWKDPLTFKPERFLESSLD 423



 Score = 87.4 bits (215), Expect(2) = 4e-49
 Identities = 39/75 (52%), Positives = 52/75 (69%)
 Frame = +2

Query: 824  FRGTHFEFIPFSAGRRTCHGSNVAFKNIQIVVASLVHYFDWSLPGGLDPTKINTGSKFGI 1003
            F+G  FEF+PF AGRR C G  +A K++ +VVASL+H+ DWSLP G DP  +N   K+GI
Sbjct: 424  FKGNDFEFLPFGAGRRICPGLPMAAKHVPLVVASLIHFLDWSLPYGKDPLDMNMSEKYGI 483

Query: 1004 VLKKEKQLCLTPSLR 1048
             L KE+ L L P ++
Sbjct: 484  TLIKEEPLILIPKVK 498