BLASTX nr result
ID: Rehmannia31_contig00016184
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia31_contig00016184
(540 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_011082655.1| nuclear/nucleolar GTPase 2 [Sesamum indicum] 148 9e-39
gb|PIN09211.1| Nucleolar GTPase [Handroanthus impetiginosus] 144 3e-37
ref|XP_022886660.1| nuclear/nucleolar GTPase 2 [Olea europaea va... 139 3e-35
emb|CDP05900.1| unnamed protein product [Coffea canephora] 132 5e-33
ref|XP_007145855.1| hypothetical protein PHAVU_007G2737000g, par... 123 2e-32
ref|XP_007133428.1| hypothetical protein PHAVU_011G1777000g, par... 123 8e-32
ref|XP_017247620.1| PREDICTED: nuclear/nucleolar GTPase 2-like [... 129 9e-32
ref|XP_019249766.1| PREDICTED: nuclear/nucleolar GTPase 2 [Nicot... 128 2e-31
ref|XP_009770289.1| PREDICTED: nucleolar GTP-binding protein 2 [... 128 2e-31
ref|XP_012847506.1| PREDICTED: nuclear/nucleolar GTPase 2 [Eryth... 127 6e-31
ref|XP_010062885.1| PREDICTED: nuclear/nucleolar GTPase 2 [Eucal... 127 9e-31
emb|CAN68109.1| hypothetical protein VITISV_000763 [Vitis vinifera] 126 9e-31
ref|XP_002284849.1| PREDICTED: nuclear/nucleolar GTPase 2 [Vitis... 126 9e-31
ref|XP_007149589.1| hypothetical protein PHAVU_005G082600g [Phas... 122 1e-30
ref|XP_009616873.1| PREDICTED: nuclear/nucleolar GTPase 2 [Nicot... 126 1e-30
gb|OAY52108.1| hypothetical protein MANES_04G058400 [Manihot esc... 121 2e-30
ref|XP_015082281.1| PREDICTED: nuclear/nucleolar GTPase 2-like [... 125 2e-30
ref|XP_006348237.1| PREDICTED: nuclear/nucleolar GTPase 2-like [... 125 2e-30
ref|XP_008386145.1| PREDICTED: nuclear/nucleolar GTPase 2 [Malus... 125 3e-30
gb|PHU12619.1| Nuclear/nucleolar GTPase 2 [Capsicum chinense] 124 4e-30
>ref|XP_011082655.1| nuclear/nucleolar GTPase 2 [Sesamum indicum]
Length = 526
Score = 148 bits (373), Expect = 9e-39
Identities = 73/99 (73%), Positives = 82/99 (82%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASHGLGGKEKAVDDDKASAA 182
+GKLLKGGEPD MTAAK+ILHDWQRGKIPFFVPPPKQE + K+K VDDDK SAA
Sbjct: 427 TGKLLKGGEPDLMTAAKVILHDWQRGKIPFFVPPPKQEADVDEPEEEKDKGVDDDKDSAA 486
Query: 183 RRAIDDLISSQQIDNVPIQKDLFSEQELTGDILETKPPA 299
R+AI D+ISSQQID+VPIQ+DLFSEQEL G+ LE P A
Sbjct: 487 RKAIKDVISSQQIDDVPIQEDLFSEQELRGETLELPPTA 525
>gb|PIN09211.1| Nucleolar GTPase [Handroanthus impetiginosus]
Length = 528
Score = 144 bits (362), Expect = 3e-37
Identities = 71/95 (74%), Positives = 82/95 (86%), Gaps = 4/95 (4%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASHGLG----GKEKAVDDDK 170
+GKLLKGGEPD+MTAAKMILHDWQRGKIPFFVPPPKQED+ S K++AVDDDK
Sbjct: 427 TGKLLKGGEPDYMTAAKMILHDWQRGKIPFFVPPPKQEDDISEQPNEVEEEKDEAVDDDK 486
Query: 171 ASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGD 275
A AAR+AI D++SSQQID+VPIQ+DLFSEQEL G+
Sbjct: 487 ACAARKAIKDVVSSQQIDDVPIQEDLFSEQELRGE 521
>ref|XP_022886660.1| nuclear/nucleolar GTPase 2 [Olea europaea var. sylvestris]
ref|XP_022886661.1| nuclear/nucleolar GTPase 2 [Olea europaea var. sylvestris]
ref|XP_022886662.1| nuclear/nucleolar GTPase 2 [Olea europaea var. sylvestris]
Length = 529
Score = 139 bits (349), Expect = 3e-35
Identities = 69/101 (68%), Positives = 83/101 (82%), Gaps = 4/101 (3%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASHGL----GGKEKAVDDDK 170
+GKLLKGGEPD TAAKMILHDWQRGKIPFFVPPPKQEDE++ K++AVDDDK
Sbjct: 425 TGKLLKGGEPDLKTAAKMILHDWQRGKIPFFVPPPKQEDESTQDPIEFDVEKDEAVDDDK 484
Query: 171 ASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGDILETKP 293
ASAAR+AI D+ISSQQ+ +VP+Q+DLF+E EL G+ +E P
Sbjct: 485 ASAARKAIADVISSQQLKDVPVQEDLFTENELIGEAVEKLP 525
>emb|CDP05900.1| unnamed protein product [Coffea canephora]
Length = 531
Score = 132 bits (333), Expect = 5e-33
Identities = 64/101 (63%), Positives = 82/101 (81%), Gaps = 4/101 (3%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASHGLG----GKEKAVDDDK 170
SGKLL+GGEPD MTAAKM+LHDWQRGKIPFFVPPPK+E+++S G +KAV+DD+
Sbjct: 427 SGKLLRGGEPDLMTAAKMVLHDWQRGKIPFFVPPPKEENDSSEGPNESGLENDKAVNDDE 486
Query: 171 ASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGDILETKP 293
SAAR+AI D+ISSQQ+ +VP+Q+ LF+E EL G+ +E P
Sbjct: 487 VSAARKAIADVISSQQLKDVPVQEGLFTENELKGNDIEQLP 527
>ref|XP_007145855.1| hypothetical protein PHAVU_007G2737000g, partial [Phaseolus
vulgaris]
gb|ESW17849.1| hypothetical protein PHAVU_007G2737000g, partial [Phaseolus
vulgaris]
Length = 170
Score = 123 bits (309), Expect = 2e-32
Identities = 62/100 (62%), Positives = 78/100 (78%), Gaps = 3/100 (3%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASH-GLGGK--EKAVDDDKA 173
SGKLLKGGEPD MT AKM+LHDWQRG+IPFFVPPPKQE+E+ + G + AVD ++A
Sbjct: 48 SGKLLKGGEPDLMTGAKMVLHDWQRGRIPFFVPPPKQEEESEEPNVNGVDIDDAVDGNEA 107
Query: 174 SAARRAIDDLISSQQIDNVPIQKDLFSEQELTGDILETKP 293
SAA +AI D++SSQQ +VP+QKDL+SE EL G+ + P
Sbjct: 108 SAAIKAIADVLSSQQQISVPVQKDLYSENELKGEATDQTP 147
>ref|XP_007133428.1| hypothetical protein PHAVU_011G1777000g, partial [Phaseolus
vulgaris]
gb|ESW05422.1| hypothetical protein PHAVU_011G1777000g, partial [Phaseolus
vulgaris]
Length = 227
Score = 123 bits (309), Expect = 8e-32
Identities = 62/100 (62%), Positives = 78/100 (78%), Gaps = 3/100 (3%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASH-GLGGK--EKAVDDDKA 173
SGKLLKGGEPD MT AKM+LHDWQRG+IPFFVPPPKQE+E+ + G + AVD ++A
Sbjct: 95 SGKLLKGGEPDLMTGAKMVLHDWQRGRIPFFVPPPKQEEESEEPNVNGVDIDDAVDGNEA 154
Query: 174 SAARRAIDDLISSQQIDNVPIQKDLFSEQELTGDILETKP 293
SAA +AI D++SSQQ +VP+QKDL+SE EL G+ + P
Sbjct: 155 SAAIKAIADVLSSQQQISVPVQKDLYSENELKGEATDQTP 194
>ref|XP_017247620.1| PREDICTED: nuclear/nucleolar GTPase 2-like [Daucus carota subsp.
sativus]
gb|KZM99548.1| hypothetical protein DCAR_013090 [Daucus carota subsp. sativus]
Length = 535
Score = 129 bits (324), Expect = 9e-32
Identities = 63/101 (62%), Positives = 79/101 (78%), Gaps = 4/101 (3%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASHGLGG----KEKAVDDDK 170
+GKLLKGGEPD MTA KMILHDWQRGKIPFFVPPPK E+E+S G ++K ++DD+
Sbjct: 431 TGKLLKGGEPDLMTAGKMILHDWQRGKIPFFVPPPKLEEESSTEPAGPGLEQDKVIEDDQ 490
Query: 171 ASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGDILETKP 293
+AAR+AI D+ISSQQ+ VP+Q++LFSE EL G+ E P
Sbjct: 491 QAAARKAIADVISSQQLKEVPVQEELFSETELRGETTEPCP 531
>ref|XP_019249766.1| PREDICTED: nuclear/nucleolar GTPase 2 [Nicotiana attenuata]
gb|OIT00432.1| nuclearnucleolar gtpase 2 [Nicotiana attenuata]
Length = 520
Score = 128 bits (321), Expect = 2e-31
Identities = 60/95 (63%), Positives = 79/95 (83%), Gaps = 4/95 (4%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASHG----LGGKEKAVDDDK 170
+GKLLKGGEPD+MTAAKM+LHDWQRGKIPFFVPPPK +D+AS + + AVDDD+
Sbjct: 425 TGKLLKGGEPDYMTAAKMVLHDWQRGKIPFFVPPPKLDDDASDEQNELVSEADTAVDDDQ 484
Query: 171 ASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGD 275
A+AA++AI D++SSQQ+ VP+Q++LFS+ EL G+
Sbjct: 485 AAAAKKAIADVVSSQQLKEVPVQEELFSQTELLGE 519
>ref|XP_009770289.1| PREDICTED: nucleolar GTP-binding protein 2 [Nicotiana sylvestris]
ref|XP_016491910.1| PREDICTED: nuclear/nucleolar GTPase 2-like [Nicotiana tabacum]
Length = 520
Score = 128 bits (321), Expect = 2e-31
Identities = 60/95 (63%), Positives = 79/95 (83%), Gaps = 4/95 (4%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASHG----LGGKEKAVDDDK 170
+GKLLKGGEPD+MTAAKM+LHDWQRGKIPFFVPPPK +D+AS + + AVDDD+
Sbjct: 425 TGKLLKGGEPDYMTAAKMVLHDWQRGKIPFFVPPPKLDDDASDEQNELVSEADTAVDDDQ 484
Query: 171 ASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGD 275
A+AA++AI D++SSQQ+ VP+Q++LFS+ EL G+
Sbjct: 485 AAAAKKAIADVVSSQQLKEVPVQEELFSQTELLGE 519
>ref|XP_012847506.1| PREDICTED: nuclear/nucleolar GTPase 2 [Erythranthe guttata]
gb|EYU44894.1| hypothetical protein MIMGU_mgv1a004445mg [Erythranthe guttata]
Length = 526
Score = 127 bits (318), Expect = 6e-31
Identities = 65/89 (73%), Positives = 74/89 (83%), Gaps = 1/89 (1%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQ-EDEASHGLGGKEKAVDDDKASA 179
SGKLLKGGEPD MT AKMILHDWQRGKIPFFV PPKQ EDE + KEK V+DD A+A
Sbjct: 427 SGKLLKGGEPDLMTGAKMILHDWQRGKIPFFVEPPKQLEDEE---IEDKEKTVEDDMAAA 483
Query: 180 ARRAIDDLISSQQIDNVPIQKDLFSEQEL 266
ARRAI+++ISSQ+ID+ P+QKDLF E EL
Sbjct: 484 ARRAINNVISSQKIDDFPVQKDLFDEDEL 512
>ref|XP_010062885.1| PREDICTED: nuclear/nucleolar GTPase 2 [Eucalyptus grandis]
gb|KCW70026.1| hypothetical protein EUGRSUZ_F03333 [Eucalyptus grandis]
Length = 573
Score = 127 bits (318), Expect = 9e-31
Identities = 67/99 (67%), Positives = 78/99 (78%), Gaps = 5/99 (5%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDE-----ASHGLGGKEKAVDDD 167
SGKLLKGGEPD TAAKM+LHDWQRGKIPFFVPPP+QE E AS+G+ KE AVD D
Sbjct: 423 SGKLLKGGEPDLTTAAKMVLHDWQRGKIPFFVPPPQQEKELPEEPASYGV-EKEAAVDSD 481
Query: 168 KASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGDILE 284
KASAA RAI +I+SQQ +VP+Q+DLF+E EL G+ E
Sbjct: 482 KASAAFRAIASVITSQQQKSVPVQRDLFTEDELKGEPAE 520
>emb|CAN68109.1| hypothetical protein VITISV_000763 [Vitis vinifera]
Length = 530
Score = 126 bits (317), Expect = 9e-31
Identities = 65/96 (67%), Positives = 77/96 (80%), Gaps = 5/96 (5%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQE-----DEASHGLGGKEKAVDDD 167
+GKLLKGGEPD MT AKM+LHDWQRG+IPFFVPPP+Q+ DE S K+ AVDD+
Sbjct: 425 TGKLLKGGEPDLMTVAKMVLHDWQRGRIPFFVPPPRQQEGDSSDEPSTLGVEKDTAVDDN 484
Query: 168 KASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGD 275
+ASAA RAI ++ISSQQ NVP+QKDLFSE+EL GD
Sbjct: 485 QASAAIRAIANVISSQQTMNVPVQKDLFSERELEGD 520
>ref|XP_002284849.1| PREDICTED: nuclear/nucleolar GTPase 2 [Vitis vinifera]
ref|XP_010644203.1| PREDICTED: nuclear/nucleolar GTPase 2 [Vitis vinifera]
Length = 530
Score = 126 bits (317), Expect = 9e-31
Identities = 65/96 (67%), Positives = 77/96 (80%), Gaps = 5/96 (5%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQE-----DEASHGLGGKEKAVDDD 167
+GKLLKGGEPD MT AKM+LHDWQRG+IPFFVPPP+Q+ DE S K+ AVDD+
Sbjct: 425 TGKLLKGGEPDLMTVAKMVLHDWQRGRIPFFVPPPRQQEGDSSDEPSTLGVEKDTAVDDN 484
Query: 168 KASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGD 275
+ASAA RAI ++ISSQQ NVP+QKDLFSE+EL GD
Sbjct: 485 QASAAIRAIANVISSQQTMNVPVQKDLFSERELEGD 520
>ref|XP_007149589.1| hypothetical protein PHAVU_005G082600g [Phaseolus vulgaris]
gb|ESW21583.1| hypothetical protein PHAVU_005G082600g [Phaseolus vulgaris]
Length = 306
Score = 122 bits (307), Expect = 1e-30
Identities = 61/100 (61%), Positives = 78/100 (78%), Gaps = 3/100 (3%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASH-GLGGK--EKAVDDDKA 173
SGKLLKGGEPD MT AKM+LHDWQRG++PFFVPPPKQE+E+ + G + AVD ++A
Sbjct: 177 SGKLLKGGEPDLMTGAKMVLHDWQRGRLPFFVPPPKQEEESEEPNVNGVDIDDAVDGNEA 236
Query: 174 SAARRAIDDLISSQQIDNVPIQKDLFSEQELTGDILETKP 293
SAA +AI D++SSQQ +VP+QKDL+SE EL G+ + P
Sbjct: 237 SAAIKAIADVLSSQQQISVPVQKDLYSENELKGEATDQAP 276
>ref|XP_009616873.1| PREDICTED: nuclear/nucleolar GTPase 2 [Nicotiana tomentosiformis]
ref|XP_016504582.1| PREDICTED: nuclear/nucleolar GTPase 2-like [Nicotiana tabacum]
Length = 520
Score = 126 bits (316), Expect = 1e-30
Identities = 59/95 (62%), Positives = 78/95 (82%), Gaps = 4/95 (4%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQE----DEASHGLGGKEKAVDDDK 170
+GKLLKGGEPD+MTAAKM+LHDWQRGKIPFFVPPPK + DE + + + AVDDD+
Sbjct: 425 TGKLLKGGEPDYMTAAKMVLHDWQRGKIPFFVPPPKLDIDASDEQNELVSEADTAVDDDQ 484
Query: 171 ASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGD 275
A+AA++AI D++SSQQ+ VP+Q++LFS+ EL G+
Sbjct: 485 AAAAKKAIADVVSSQQLKEVPVQEELFSQNELLGE 519
>gb|OAY52108.1| hypothetical protein MANES_04G058400 [Manihot esculenta]
Length = 282
Score = 121 bits (304), Expect = 2e-30
Identities = 62/96 (64%), Positives = 77/96 (80%), Gaps = 6/96 (6%)
Frame = +3
Query: 6 GKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEAS------HGLGGKEKAVDDD 167
GKLL+GGEPD MTAAKM+LHDWQRG+IPFFVPPP+Q+++ S G+ +E A DD+
Sbjct: 145 GKLLEGGEPDLMTAAKMVLHDWQRGRIPFFVPPPRQQEDDSLEEPNADGI-NEETAGDDN 203
Query: 168 KASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGD 275
+ASAA RAI ++ISSQQ NVP+Q+DLFSE EL GD
Sbjct: 204 QASAAFRAIVNVISSQQQKNVPVQRDLFSENELRGD 239
>ref|XP_015082281.1| PREDICTED: nuclear/nucleolar GTPase 2-like [Solanum pennellii]
Length = 516
Score = 125 bits (314), Expect = 2e-30
Identities = 58/91 (63%), Positives = 75/91 (82%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASHGLGGKEKAVDDDKASAA 182
SGKLLKGGEPD+ TAAKM+LHDWQRG IPFFVPPPK +DE + + + AVD+D+A+AA
Sbjct: 425 SGKLLKGGEPDYNTAAKMVLHDWQRGNIPFFVPPPKLDDEPNELVAEADTAVDNDQATAA 484
Query: 183 RRAIDDLISSQQIDNVPIQKDLFSEQELTGD 275
RAI D++SSQQ+ VP+Q++LFS+ EL G+
Sbjct: 485 LRAIADVVSSQQLKEVPVQEELFSKAELLGE 515
>ref|XP_006348237.1| PREDICTED: nuclear/nucleolar GTPase 2-like [Solanum tuberosum]
Length = 516
Score = 125 bits (314), Expect = 2e-30
Identities = 57/91 (62%), Positives = 76/91 (83%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASHGLGGKEKAVDDDKASAA 182
+GKLLKGGEPD+ TAAKM+LHDWQRGKIPFFVPPPK +DE + + + AVD+D+A+AA
Sbjct: 425 TGKLLKGGEPDYKTAAKMVLHDWQRGKIPFFVPPPKLDDEQNELVAEADTAVDNDQATAA 484
Query: 183 RRAIDDLISSQQIDNVPIQKDLFSEQELTGD 275
+AI D++SSQQ+ VP+Q++LFS+ EL G+
Sbjct: 485 LKAIADVVSSQQLKEVPVQEELFSKAELLGE 515
>ref|XP_008386145.1| PREDICTED: nuclear/nucleolar GTPase 2 [Malus domestica]
Length = 562
Score = 125 bits (314), Expect = 3e-30
Identities = 63/106 (59%), Positives = 78/106 (73%), Gaps = 5/106 (4%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQED-----EASHGLGGKEKAVDDD 167
SGKLLKGGEPD MTAAKM+LHDWQRGKIPFFVPPPK+E + +H KE V+D+
Sbjct: 428 SGKLLKGGEPDLMTAAKMVLHDWQRGKIPFFVPPPKRESPTGEPDINH---NKEDTVEDN 484
Query: 168 KASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGDILETKPPAVE 305
+AA RAI D+I+SQ++ +P+Q+DLFSE+EL GD PA E
Sbjct: 485 NQAAAYRAIGDIIASQKVSTIPVQRDLFSEEELRGDEGPEHLPAAE 530
>gb|PHU12619.1| Nuclear/nucleolar GTPase 2 [Capsicum chinense]
Length = 520
Score = 124 bits (312), Expect = 4e-30
Identities = 60/95 (63%), Positives = 76/95 (80%), Gaps = 4/95 (4%)
Frame = +3
Query: 3 SGKLLKGGEPDFMTAAKMILHDWQRGKIPFFVPPPKQEDEASHG----LGGKEKAVDDDK 170
+GKLLKGGEPDF TAAKMILHDWQRGKIPFFVPPPK +D+AS + + VD+D+
Sbjct: 425 TGKLLKGGEPDFKTAAKMILHDWQRGKIPFFVPPPKLDDDASDEQNVLVSEADPTVDEDQ 484
Query: 171 ASAARRAIDDLISSQQIDNVPIQKDLFSEQELTGD 275
A AA++AI D++SSQQ+ VP+Q+DLFS+ EL G+
Sbjct: 485 AMAAKKAIADVVSSQQLKEVPVQEDLFSKTELLGE 519