BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00016104
         (1234 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_012838234.1| PREDICTED: nicastrin [Erythranthe guttata] >...   737   0.0  
gb|PIN06941.1| Transmembrane glycoprotein nicastrin [Handroanthu...   701   0.0  
ref|XP_011085876.1| nicastrin [Sesamum indicum]                       692   0.0  
ref|XP_022884594.1| nicastrin isoform X2 [Olea europaea var. syl...   636   0.0  
ref|XP_022843240.1| nicastrin-like [Olea europaea var. sylvestris]    629   0.0  
ref|XP_022884595.1| nicastrin isoform X3 [Olea europaea var. syl...   628   0.0  
ref|XP_022884593.1| nicastrin isoform X1 [Olea europaea var. syl...   628   0.0  
gb|KZV53792.1| nicastrin [Dorcoceras hygrometricum]                   622   0.0  
ref|XP_009758540.1| PREDICTED: nicastrin isoform X2 [Nicotiana s...   595   0.0  
ref|XP_019223514.1| PREDICTED: nicastrin isoform X2 [Nicotiana a...   593   0.0  
ref|XP_019223517.1| PREDICTED: nicastrin isoform X5 [Nicotiana a...   589   0.0  
ref|XP_008368738.1| PREDICTED: nicastrin-like [Malus domestica]       585   0.0  
ref|XP_009758539.1| PREDICTED: nicastrin isoform X1 [Nicotiana s...   591   0.0  
ref|XP_019223515.1| PREDICTED: nicastrin isoform X3 [Nicotiana a...   589   0.0  
ref|XP_002264144.1| PREDICTED: nicastrin [Vitis vinifera] >gi|29...   590   0.0  
emb|CDP01653.1| unnamed protein product [Coffea canephora]            588   0.0  
ref|XP_019223513.1| PREDICTED: nicastrin isoform X1 [Nicotiana a...   589   0.0  
ref|XP_006352434.1| PREDICTED: nicastrin isoform X2 [Solanum tub...   588   0.0  
ref|XP_016488669.1| PREDICTED: nicastrin-like isoform X2 [Nicoti...   588   0.0  
ref|XP_009626875.1| PREDICTED: nicastrin isoform X2 [Nicotiana t...   588   0.0  

>ref|XP_012838234.1| PREDICTED: nicastrin [Erythranthe guttata]
 gb|EYU45769.1| hypothetical protein MIMGU_mgv1a002472mg [Erythranthe guttata]
          Length = 669

 Score =  737 bits (1903), Expect = 0.0
 Identities = 366/410 (89%), Positives = 393/410 (95%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            E++K +Y KVVSEFDLVMQTTKSGT DS+SCLKEGTCLPLGGYSVWSALPPINISSP+KA
Sbjct: 187  EESKSSYPKVVSEFDLVMQTTKSGTRDSNSCLKEGTCLPLGGYSVWSALPPINISSPVKA 246

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KP+VLTMTSMDSASFFRDKNLGAE+PISGLIALLAVVDALS   GL++LNKQLVFVV TG
Sbjct: 247  KPIVLTMTSMDSASFFRDKNLGAETPISGLIALLAVVDALSHFDGLKDLNKQLVFVVFTG 306

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRFFLES+QQSDT+KGLDLAMIETV+EIGSVGKS+P+ +KTFFAHTAGA SS
Sbjct: 307  EAWGYLGSRRFFLESEQQSDTIKGLDLAMIETVMEIGSVGKSFPESSKTFFAHTAGAKSS 366

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
            +NGTLNALQ AQ+SL TESI+VKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS
Sbjct: 367  LNGTLNALQSAQESLKTESIIVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 426

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDPSAINNIKINSSFVEELLGCLL 333
            NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDPSAI++IKIN+S V+ELLGCLL
Sbjct: 427  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDPSAISSIKINASLVDELLGCLL 486

Query: 332  DCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKTSIP 153
            +CEPGLSCGLVK YISPSTTCP+HYVGVILGEPSS+PYPAYAGDISRFVWNFLADKTSIP
Sbjct: 487  NCEPGLSCGLVKRYISPSTTCPNHYVGVILGEPSSEPYPAYAGDISRFVWNFLADKTSIP 546

Query: 152  SKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            SKNVSSSC KDC GD ELCIREEADGKGVCV+STTRFVPAYSTRLKY+ E
Sbjct: 547  SKNVSSSCPKDCKGDGELCIREEADGKGVCVVSTTRFVPAYSTRLKYESE 596


>gb|PIN06941.1| Transmembrane glycoprotein nicastrin [Handroanthus impetiginosus]
          Length = 595

 Score =  701 bits (1809), Expect = 0.0
 Identities = 347/409 (84%), Positives = 380/409 (92%)
 Frame = -3

Query: 1229 KTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKAK 1050
            K KK+Y + V+EFDLVMQTTKSGTH+SDSCLKEG+CLPLGGYSVWSALPPINIS   KAK
Sbjct: 148  KNKKSYIETVAEFDLVMQTTKSGTHNSDSCLKEGSCLPLGGYSVWSALPPINISYSPKAK 207

Query: 1049 PVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTGE 870
            P+VLTMTSMDSASFFRDKNLGA+SPISGLIALLAVVDALSRL GL+EL+KQLVF+VLTGE
Sbjct: 208  PIVLTMTSMDSASFFRDKNLGAQSPISGLIALLAVVDALSRLDGLQELHKQLVFMVLTGE 267

Query: 869  AWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSSV 690
            AWGY+GSRR FLE DQQSD+MKGLDLA I+TV+EIGSVGK  PQG  TFFAH +GATSSV
Sbjct: 268  AWGYIGSRRLFLEFDQQSDSMKGLDLATIDTVLEIGSVGKGSPQGINTFFAHASGATSSV 327

Query: 689  NGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFSN 510
            NGTL+AL+ AQD+L  ESI+VKTASTSNPGVPPSSLMTFLRKKP+ISGLVLEDFDSAFSN
Sbjct: 328  NGTLDALKRAQDALRNESIIVKTASTSNPGVPPSSLMTFLRKKPKISGLVLEDFDSAFSN 387

Query: 509  EFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDPSAINNIKINSSFVEELLGCLLD 330
            +FYHSHLDDLSNINS+SIVAAASLV+R+LY LAGGKD S +NNIKINSS VEELLGCLLD
Sbjct: 388  KFYHSHLDDLSNINSSSIVAAASLVSRTLYTLAGGKDSSVMNNIKINSSLVEELLGCLLD 447

Query: 329  CEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKTSIPS 150
            CEPGLSCGLVKHYISP+TTCPSHYVGVILGEPSS+PYP YAGDISRFVWNFLADKTSI S
Sbjct: 448  CEPGLSCGLVKHYISPATTCPSHYVGVILGEPSSEPYPPYAGDISRFVWNFLADKTSIQS 507

Query: 149  KNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            +N SSSC KDC+GD ELCIR+EADGKGVCVISTTR+VPAYS RLKY+ +
Sbjct: 508  RNGSSSCPKDCSGDGELCIRQEADGKGVCVISTTRYVPAYSARLKYESD 556


>ref|XP_011085876.1| nicastrin [Sesamum indicum]
          Length = 668

 Score =  692 bits (1786), Expect = 0.0
 Identities = 346/408 (84%), Positives = 376/408 (92%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK++ +YT+ V+EFDLVMQTTKS THDS+SCLKEGTCLPLGGYSVWSALPPINISS LKA
Sbjct: 187  EKSEMSYTENVAEFDLVMQTTKSSTHDSESCLKEGTCLPLGGYSVWSALPPINISSSLKA 246

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KP++L MTSMDSASFFRD++LGAESPISGLIALLAVVDALS L GLEELNKQLVFVVLTG
Sbjct: 247  KPIILAMTSMDSASFFRDRSLGAESPISGLIALLAVVDALSHLDGLEELNKQLVFVVLTG 306

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            E+WGYLGSRRFFLESD+QSD MKGLDLAM+E V+EIGSVGKS+ QG KTFFAHTAGA SS
Sbjct: 307  ESWGYLGSRRFFLESDKQSDAMKGLDLAMLEMVLEIGSVGKSFSQGNKTFFAHTAGAASS 366

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
            VNGT N LQ AQDSL+TESIMVK AST+NPGVPPSSLMTFLRKKPQISG VLEDFDS FS
Sbjct: 367  VNGTFNVLQRAQDSLVTESIMVKRASTTNPGVPPSSLMTFLRKKPQISGFVLEDFDSTFS 426

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDPSAINNIKINSSFVEELLGCLL 333
            N+FYHSHLD+LSNI+ +SIVAAASLVAR+LYILAGGKD SAIN IK+N+S VEELLGCLL
Sbjct: 427  NKFYHSHLDELSNISPSSIVAAASLVARTLYILAGGKD-SAINTIKVNASLVEELLGCLL 485

Query: 332  DCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKTSIP 153
             CEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSS  YPAYAGD+SRFVWNFLADKTSI 
Sbjct: 486  ACEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSALYPAYAGDVSRFVWNFLADKTSIS 545

Query: 152  SKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYD 9
             +NV SSC+K C+G  ELCIR EADGKGVCVISTTR+VPAYSTRLKY+
Sbjct: 546  LRNVGSSCAKACSGVGELCIRGEADGKGVCVISTTRYVPAYSTRLKYE 593


>ref|XP_022884594.1| nicastrin isoform X2 [Olea europaea var. sylvestris]
          Length = 667

 Score =  636 bits (1641), Expect = 0.0
 Identities = 312/408 (76%), Positives = 359/408 (87%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EKTKK+ T+ V+EF++VMQTTKSGTHDSDSCLKE TCLPLGGYSVWSALPPINISS  K+
Sbjct: 185  EKTKKSNTENVAEFNVVMQTTKSGTHDSDSCLKEETCLPLGGYSVWSALPPINISSSQKS 244

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV++TMTSMDSASFFRDK+LGA+SPISGLI LLA VDALS L GL+ELNKQLVFVVLTG
Sbjct: 245  KPVIVTMTSMDSASFFRDKSLGADSPISGLITLLAAVDALSYLDGLDELNKQLVFVVLTG 304

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            E+WGYLGSRRF LE+DQ SD+++GLD AMIETV+EIGSVGK    G KTF++HT+G T +
Sbjct: 305  ESWGYLGSRRFVLEADQHSDSVRGLDFAMIETVMEIGSVGKGLSNGVKTFYSHTSGDTLA 364

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             N T  AL  AQDSL TESI +  ASTSNPGVPPSSLMTFLRKK QISG+VLEDFD++F+
Sbjct: 365  TNETFQALLRAQDSLKTESIKISMASTSNPGVPPSSLMTFLRKKSQISGVVLEDFDTSFT 424

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDPSAINNIKINSSFVEELLGCLL 333
            N+FYHSHLDDLSN+NS++IV AASLVAR+LY+LA  ++ SAIN I +N+S VEELLGCLL
Sbjct: 425  NKFYHSHLDDLSNVNSSAIVTAASLVARTLYLLANDQNASAINAINVNASLVEELLGCLL 484

Query: 332  DCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKTSIP 153
            +C+PG SC L+KHYISPS TCPSHYVGVILGEPSS PYPAY  D+SRFVWNFLA KTSI 
Sbjct: 485  NCKPGSSCELMKHYISPSATCPSHYVGVILGEPSSVPYPAYVSDVSRFVWNFLASKTSIF 544

Query: 152  SKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYD 9
            S+N++S+C  DCNG  ELCIREEADGKG+CVISTTR+VPAYSTRLKY+
Sbjct: 545  SRNITSACPNDCNGVGELCIREEADGKGICVISTTRYVPAYSTRLKYE 592


>ref|XP_022843240.1| nicastrin-like [Olea europaea var. sylvestris]
          Length = 591

 Score =  629 bits (1623), Expect = 0.0
 Identities = 309/408 (75%), Positives = 357/408 (87%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EKTKK  T+ V+EFD+VMQTTKSGTHDSDSCLKE TCLPLGGYSVWSALPPINI S  K+
Sbjct: 109  EKTKKFNTENVAEFDVVMQTTKSGTHDSDSCLKEETCLPLGGYSVWSALPPINILSSQKS 168

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+L +TSMDSASFFRDK+LGA+SPISGLI LLA VDALS L GL+ELNKQLVFVVLTG
Sbjct: 169  KPVILAITSMDSASFFRDKSLGADSPISGLITLLAAVDALSHLDGLDELNKQLVFVVLTG 228

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            E+WGYLGSRRF LE+D+ SD+++ L+LAM ETV+EIGSVGK    G KTF++HT GAT +
Sbjct: 229  ESWGYLGSRRFLLEADEHSDSVRDLNLAMTETVMEIGSVGKGLSNGIKTFYSHTTGATWA 288

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
            +N T +AL  AQDSL TESI +  AS SNPGVPPSSLMTFLRKK  ISG+VLEDFD++F+
Sbjct: 289  MNETWHALLRAQDSLKTESIKISMASKSNPGVPPSSLMTFLRKKSHISGVVLEDFDTSFT 348

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDPSAINNIKINSSFVEELLGCLL 333
            N+FYHSHLDDLSN+NS++IVAAASLVAR+LY+LA  ++ SAIN I +N+S V+ELLGCLL
Sbjct: 349  NKFYHSHLDDLSNVNSSAIVAAASLVARTLYLLASDQNASAINAINVNASLVKELLGCLL 408

Query: 332  DCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKTSIP 153
            +C+PGLSC LVKHYISPS TCPSHYVGV+LGEPS+ PYPAY  D+SRFVWNFLADKTSI 
Sbjct: 409  NCDPGLSCELVKHYISPSETCPSHYVGVVLGEPSALPYPAYVSDVSRFVWNFLADKTSIS 468

Query: 152  SKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYD 9
            S+N SS+C KDC G  ELCIREEADGKGVC+ISTTR+VPAYSTRLKY+
Sbjct: 469  SRNTSSACPKDCKGVGELCIREEADGKGVCIISTTRYVPAYSTRLKYE 516


>ref|XP_022884595.1| nicastrin isoform X3 [Olea europaea var. sylvestris]
 ref|XP_022884596.1| nicastrin isoform X3 [Olea europaea var. sylvestris]
          Length = 578

 Score =  628 bits (1619), Expect = 0.0
 Identities = 312/419 (74%), Positives = 359/419 (85%), Gaps = 11/419 (2%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EKTKK+ T+ V+EF++VMQTTKSGTHDSDSCLKE TCLPLGGYSVWSALPPINISS  K+
Sbjct: 85   EKTKKSNTENVAEFNVVMQTTKSGTHDSDSCLKEETCLPLGGYSVWSALPPINISSSQKS 144

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV++TMTSMDSASFFRDK+LGA+SPISGLI LLA VDALS L GL+ELNKQLVFVVLTG
Sbjct: 145  KPVIVTMTSMDSASFFRDKSLGADSPISGLITLLAAVDALSYLDGLDELNKQLVFVVLTG 204

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            E+WGYLGSRRF LE+DQ SD+++GLD AMIETV+EIGSVGK    G KTF++HT+G T +
Sbjct: 205  ESWGYLGSRRFVLEADQHSDSVRGLDFAMIETVMEIGSVGKGLSNGVKTFYSHTSGDTLA 264

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             N T  AL  AQDSL TESI +  ASTSNPGVPPSSLMTFLRKK QISG+VLEDFD++F+
Sbjct: 265  TNETFQALLRAQDSLKTESIKISMASTSNPGVPPSSLMTFLRKKSQISGVVLEDFDTSFT 324

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDPSAINNIKINSSFVEELLGCLL 333
            N+FYHSHLDDLSN+NS++IV AASLVAR+LY+LA  ++ SAIN I +N+S VEELLGCLL
Sbjct: 325  NKFYHSHLDDLSNVNSSAIVTAASLVARTLYLLANDQNASAINAINVNASLVEELLGCLL 384

Query: 332  DCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKTSIP 153
            +C+PG SC L+KHYISPS TCPSHYVGVILGEPSS PYPAY  D+SRFVWNFLA KTSI 
Sbjct: 385  NCKPGSSCELMKHYISPSATCPSHYVGVILGEPSSVPYPAYVSDVSRFVWNFLASKTSIF 444

Query: 152  SKNVSSSCSKDCNGDNELCIREEADGKGVCVIST-----------TRFVPAYSTRLKYD 9
            S+N++S+C  DCNG  ELCIREEADGKG+CVIST           TR+VPAYSTRLKY+
Sbjct: 445  SRNITSACPNDCNGVGELCIREEADGKGICVISTTRCLFLPFFYFTRYVPAYSTRLKYE 503


>ref|XP_022884593.1| nicastrin isoform X1 [Olea europaea var. sylvestris]
          Length = 678

 Score =  628 bits (1619), Expect = 0.0
 Identities = 312/419 (74%), Positives = 359/419 (85%), Gaps = 11/419 (2%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EKTKK+ T+ V+EF++VMQTTKSGTHDSDSCLKE TCLPLGGYSVWSALPPINISS  K+
Sbjct: 185  EKTKKSNTENVAEFNVVMQTTKSGTHDSDSCLKEETCLPLGGYSVWSALPPINISSSQKS 244

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV++TMTSMDSASFFRDK+LGA+SPISGLI LLA VDALS L GL+ELNKQLVFVVLTG
Sbjct: 245  KPVIVTMTSMDSASFFRDKSLGADSPISGLITLLAAVDALSYLDGLDELNKQLVFVVLTG 304

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            E+WGYLGSRRF LE+DQ SD+++GLD AMIETV+EIGSVGK    G KTF++HT+G T +
Sbjct: 305  ESWGYLGSRRFVLEADQHSDSVRGLDFAMIETVMEIGSVGKGLSNGVKTFYSHTSGDTLA 364

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             N T  AL  AQDSL TESI +  ASTSNPGVPPSSLMTFLRKK QISG+VLEDFD++F+
Sbjct: 365  TNETFQALLRAQDSLKTESIKISMASTSNPGVPPSSLMTFLRKKSQISGVVLEDFDTSFT 424

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDPSAINNIKINSSFVEELLGCLL 333
            N+FYHSHLDDLSN+NS++IV AASLVAR+LY+LA  ++ SAIN I +N+S VEELLGCLL
Sbjct: 425  NKFYHSHLDDLSNVNSSAIVTAASLVARTLYLLANDQNASAINAINVNASLVEELLGCLL 484

Query: 332  DCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKTSIP 153
            +C+PG SC L+KHYISPS TCPSHYVGVILGEPSS PYPAY  D+SRFVWNFLA KTSI 
Sbjct: 485  NCKPGSSCELMKHYISPSATCPSHYVGVILGEPSSVPYPAYVSDVSRFVWNFLASKTSIF 544

Query: 152  SKNVSSSCSKDCNGDNELCIREEADGKGVCVIST-----------TRFVPAYSTRLKYD 9
            S+N++S+C  DCNG  ELCIREEADGKG+CVIST           TR+VPAYSTRLKY+
Sbjct: 545  SRNITSACPNDCNGVGELCIREEADGKGICVISTTRCLFLPFFYFTRYVPAYSTRLKYE 603


>gb|KZV53792.1| nicastrin [Dorcoceras hygrometricum]
          Length = 669

 Score =  622 bits (1603), Expect = 0.0
 Identities = 302/408 (74%), Positives = 354/408 (86%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            +K K +YT+ V EFDLVM TTKSG  DSD+CL++G CLPLGGYSVWSALPP++ISS  K 
Sbjct: 187  DKRKTSYTEDVVEFDLVMDTTKSGNLDSDACLRDGACLPLGGYSVWSALPPVSISSSPKT 246

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KP+VLT+TSMDSASFFRDK+LGA+SPISGLI LLA VDA+S +  L+EL+KQLVF+VLTG
Sbjct: 247  KPLVLTVTSMDSASFFRDKSLGADSPISGLITLLAAVDAISHVQKLDELDKQLVFLVLTG 306

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LESDQ SD +KGLD+AM+E V+EIGSVGKS+ QG KT + HT+G TSS
Sbjct: 307  EAWGYLGSRRFLLESDQHSDAIKGLDMAMVEMVLEIGSVGKSFSQGMKTLYTHTSGDTSS 366

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
            V+GTLNA+Q AQDS  T+ I+VK ASTSNPGVPPSSLMTFLRK  QISG+VLEDFDSAF+
Sbjct: 367  VHGTLNAIQRAQDSFKTDGIVVKRASTSNPGVPPSSLMTFLRKNHQISGVVLEDFDSAFT 426

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDPSAINNIKINSSFVEELLGCLL 333
            N+FYHSHLDDLSN+NS++IV+AA+LVAR+LYI AGG + S +++I +N+S VEELL CLL
Sbjct: 427  NKFYHSHLDDLSNVNSSAIVSAAALVARTLYIAAGGNNDSVMSSINVNASMVEELLSCLL 486

Query: 332  DCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKTSIP 153
             CEPGLSC LVKHYISPS+TCPSHYVGV+LGEPSS P PAY GD+SRFVWNFLADKTS P
Sbjct: 487  KCEPGLSCDLVKHYISPSSTCPSHYVGVVLGEPSSAPNPAYVGDVSRFVWNFLADKTSTP 546

Query: 152  SKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYD 9
            SK+ SS+C  DC    ELCI+ E DGKGVCV+STTR+VPAYSTRLKY+
Sbjct: 547  SKSTSSACPNDCKDVGELCIKTENDGKGVCVVSTTRYVPAYSTRLKYE 594


>ref|XP_009758540.1| PREDICTED: nicastrin isoform X2 [Nicotiana sylvestris]
 ref|XP_016500129.1| PREDICTED: nicastrin-like isoform X2 [Nicotiana tabacum]
          Length = 670

 Score =  595 bits (1534), Expect = 0.0
 Identities = 292/413 (70%), Positives = 350/413 (84%), Gaps = 3/413 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK KK+YT  V++F+LVMQTTKSGT DS+SCL+E TCLPLGGYSVWSALPP+ ISS  KA
Sbjct: 186  EKRKKSYTVDVADFNLVMQTTKSGTRDSESCLREQTCLPLGGYSVWSALPPMAISSSEKA 245

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+L + SMD+ASFFRDK++GA+SPISGLI+LL VVDALSR+ GL++L+KQLVF V TG
Sbjct: 246  KPVILVVASMDAASFFRDKSIGADSPISGLISLLTVVDALSRVDGLKDLDKQLVFAVFTG 305

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE DQ SD + GLDLA+IETV+EIGSVGK + Q  KTFFAH+   T++
Sbjct: 306  EAWGYLGSRRFLLELDQHSDAVSGLDLALIETVLEIGSVGKGFAQDDKTFFAHSTSETAT 365

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             NGTL+A+Q A  SL T+SI +  AS SNPG+PPSSLM+FL+K P+ SG+VLEDFD+AF+
Sbjct: 366  -NGTLSAIQDALGSLRTQSIKISRASKSNPGLPPSSLMSFLKKNPKTSGVVLEDFDTAFT 424

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGK---DPSAINNIKINSSFVEELLG 342
            N+FYHSHLDDL NINS++IVAAAS+VARSLYILA  K     SA+N I IN+S VEELLG
Sbjct: 425  NKFYHSHLDDLLNINSSAIVAAASVVARSLYILASNKKEIKTSALNTININASLVEELLG 484

Query: 341  CLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKT 162
            CLL CEPGLSC LV  YI+PST+CPSHYVGV+LGEPSS+PYP   GD+ RFVWNFLADKT
Sbjct: 485  CLLSCEPGLSCELVNRYIAPSTSCPSHYVGVVLGEPSSQPYPGNVGDVPRFVWNFLADKT 544

Query: 161  SIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            +IPSKN+SS+C K C+G  E+C++ E DGKGVCVISTTR++PAYSTRLKY+ +
Sbjct: 545  AIPSKNLSSTCPKGCSGKGEMCVKAETDGKGVCVISTTRYIPAYSTRLKYESD 597


>ref|XP_019223514.1| PREDICTED: nicastrin isoform X2 [Nicotiana attenuata]
          Length = 670

 Score =  593 bits (1530), Expect = 0.0
 Identities = 291/413 (70%), Positives = 351/413 (84%), Gaps = 3/413 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK KK+YT  V++F+LVMQTTKSGT DS+SCL+E TCLPLGGYSVWSALPP+ ISS  KA
Sbjct: 186  EKRKKSYTVDVADFNLVMQTTKSGTRDSESCLREQTCLPLGGYSVWSALPPMAISSSEKA 245

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+L + SMD+ASFFRDK++GA+SPISGLI+LLAVVDALSR+ GL++L+KQLVF V TG
Sbjct: 246  KPVILVVASMDAASFFRDKSIGADSPISGLISLLAVVDALSRVDGLKDLDKQLVFAVFTG 305

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE DQ SD + GLDLA+IETV+EIGSVGK + +  KTFFAH+   T++
Sbjct: 306  EAWGYLGSRRFLLELDQHSDAVSGLDLALIETVLEIGSVGKGFARDDKTFFAHSTSETAT 365

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             NGTL+A+Q A  SL T+S  +  AS SNPG+PPSSLM+FL+K P+ SG+VLEDFD+AF+
Sbjct: 366  -NGTLSAIQDAVGSLKTQSTKISRASKSNPGLPPSSLMSFLKKNPKTSGVVLEDFDTAFT 424

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGK---DPSAINNIKINSSFVEELLG 342
            N+FYHSHLDDL NINS++IVAAAS+VARSLYILA  K     SA+N I IN+S VEELLG
Sbjct: 425  NKFYHSHLDDLLNINSSAIVAAASVVARSLYILASDKKEIKTSALNTININASLVEELLG 484

Query: 341  CLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKT 162
            CLL CEPGLSC LV  YI+PST+CPSHYVGV+LGEPSS+PYP   GD+ RFVWNFLADKT
Sbjct: 485  CLLSCEPGLSCELVNRYIAPSTSCPSHYVGVVLGEPSSQPYPGNVGDVPRFVWNFLADKT 544

Query: 161  SIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            +IPSKN+SS+C K C+G+ E+C++ E DGKGVCVISTTR++PAYSTRLKY+ +
Sbjct: 545  AIPSKNLSSTCPKGCSGNGEMCVKAETDGKGVCVISTTRYIPAYSTRLKYESD 597


>ref|XP_019223517.1| PREDICTED: nicastrin isoform X5 [Nicotiana attenuata]
          Length = 583

 Score =  589 bits (1519), Expect = 0.0
 Identities = 291/414 (70%), Positives = 351/414 (84%), Gaps = 4/414 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK KK+YT  V++F+LVMQTTKSGT DS+SCL+E TCLPLGGYSVWSALPP+ ISS  KA
Sbjct: 98   EKRKKSYTVDVADFNLVMQTTKSGTRDSESCLREQTCLPLGGYSVWSALPPMAISSSEKA 157

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+L + SMD+ASFFRDK++GA+SPISGLI+LLAVVDALSR+ GL++L+KQLVF V TG
Sbjct: 158  KPVILVVASMDAASFFRDKSIGADSPISGLISLLAVVDALSRVDGLKDLDKQLVFAVFTG 217

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE DQ SD + GLDLA+IETV+EIGSVGK + +  KTFFAH+   T++
Sbjct: 218  EAWGYLGSRRFLLELDQHSDAVSGLDLALIETVLEIGSVGKGFARDDKTFFAHSTSETAT 277

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             NGTL+A+Q A  SL T+S  +  AS SNPG+PPSSLM+FL+K P+ SG+VLEDFD+AF+
Sbjct: 278  -NGTLSAIQDAVGSLKTQSTKISRASKSNPGLPPSSLMSFLKKNPKTSGVVLEDFDTAFT 336

Query: 512  NEFYHSHLDDLS-NINSTSIVAAASLVARSLYILAGGK---DPSAINNIKINSSFVEELL 345
            N+FYHSHLDDL  NINS++IVAAAS+VARSLYILA  K     SA+N I IN+S VEELL
Sbjct: 337  NKFYHSHLDDLPVNINSSAIVAAASVVARSLYILASDKKEIKTSALNTININASLVEELL 396

Query: 344  GCLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADK 165
            GCLL CEPGLSC LV  YI+PST+CPSHYVGV+LGEPSS+PYP   GD+ RFVWNFLADK
Sbjct: 397  GCLLSCEPGLSCELVNRYIAPSTSCPSHYVGVVLGEPSSQPYPGNVGDVPRFVWNFLADK 456

Query: 164  TSIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            T+IPSKN+SS+C K C+G+ E+C++ E DGKGVCVISTTR++PAYSTRLKY+ +
Sbjct: 457  TAIPSKNLSSTCPKGCSGNGEMCVKAETDGKGVCVISTTRYIPAYSTRLKYESD 510


>ref|XP_008368738.1| PREDICTED: nicastrin-like [Malus domestica]
          Length = 504

 Score =  585 bits (1509), Expect = 0.0
 Identities = 287/411 (69%), Positives = 341/411 (82%), Gaps = 3/411 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            +K+KK+YT  V+EFDLVMQTTKSGTHDS+SCLKE TCLPLGGYSVWS+LPPINISSP  +
Sbjct: 18   QKSKKSYTDDVAEFDLVMQTTKSGTHDSESCLKENTCLPLGGYSVWSSLPPINISSPEHS 77

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KP++L +TSMDSASFFRDK LGA+SPISGLI+LLA VDALSR   L+  NKQLVF V TG
Sbjct: 78   KPIILVLTSMDSASFFRDKALGADSPISGLISLLAAVDALSRADDLDGFNKQLVFAVFTG 137

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE D QS+ + GL+ ++IE VVEIGSVGK   QG K FF HT G +SS
Sbjct: 138  EAWGYLGSRRFLLELDLQSNAVSGLNYSLIEKVVEIGSVGKGLNQGAKNFFIHTTGVSSS 197

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             N TL+A++ AQDS+ +E+I + +A+ SNPG+PPSSLMTFLRK    SG+VLEDFD+ F+
Sbjct: 198  TNETLDAVRRAQDSVKSENITISSANASNPGIPPSSLMTFLRKNSLTSGVVLEDFDNVFT 257

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAG---GKDPSAINNIKINSSFVEELLG 342
            N+FYHSHLDDLSNINS+SI AAASLVAR+LYILA        SA+ +I +N+S VEEL+G
Sbjct: 258  NKFYHSHLDDLSNINSSSIAAAASLVARALYILASDTKNLSSSALTSINVNASLVEELVG 317

Query: 341  CLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKT 162
            CL+DCEPGLSC LVK YISP+ TCPSHYVGVIL EPSS P   Y GDISRFVWNFLA++T
Sbjct: 318  CLVDCEPGLSCDLVKSYISPANTCPSHYVGVILDEPSSTPDLGYVGDISRFVWNFLAERT 377

Query: 161  SIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYD 9
            SIP +N SS C++DC+ D E+CIR E +GKGVCV+STTR+VPAYSTRLK++
Sbjct: 378  SIPKENASSVCTQDCSNDGEVCIRAETEGKGVCVVSTTRYVPAYSTRLKFE 428


>ref|XP_009758539.1| PREDICTED: nicastrin isoform X1 [Nicotiana sylvestris]
 ref|XP_016500128.1| PREDICTED: nicastrin-like isoform X1 [Nicotiana tabacum]
          Length = 671

 Score =  591 bits (1523), Expect = 0.0
 Identities = 292/414 (70%), Positives = 350/414 (84%), Gaps = 4/414 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK KK+YT  V++F+LVMQTTKSGT DS+SCL+E TCLPLGGYSVWSALPP+ ISS  KA
Sbjct: 186  EKRKKSYTVDVADFNLVMQTTKSGTRDSESCLREQTCLPLGGYSVWSALPPMAISSSEKA 245

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+L + SMD+ASFFRDK++GA+SPISGLI+LL VVDALSR+ GL++L+KQLVF V TG
Sbjct: 246  KPVILVVASMDAASFFRDKSIGADSPISGLISLLTVVDALSRVDGLKDLDKQLVFAVFTG 305

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE DQ SD + GLDLA+IETV+EIGSVGK + Q  KTFFAH+   T++
Sbjct: 306  EAWGYLGSRRFLLELDQHSDAVSGLDLALIETVLEIGSVGKGFAQDDKTFFAHSTSETAT 365

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             NGTL+A+Q A  SL T+SI +  AS SNPG+PPSSLM+FL+K P+ SG+VLEDFD+AF+
Sbjct: 366  -NGTLSAIQDALGSLRTQSIKISRASKSNPGLPPSSLMSFLKKNPKTSGVVLEDFDTAFT 424

Query: 512  NEFYHSHLDDLS-NINSTSIVAAASLVARSLYILAGGK---DPSAINNIKINSSFVEELL 345
            N+FYHSHLDDL  NINS++IVAAAS+VARSLYILA  K     SA+N I IN+S VEELL
Sbjct: 425  NKFYHSHLDDLPVNINSSAIVAAASVVARSLYILASNKKEIKTSALNTININASLVEELL 484

Query: 344  GCLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADK 165
            GCLL CEPGLSC LV  YI+PST+CPSHYVGV+LGEPSS+PYP   GD+ RFVWNFLADK
Sbjct: 485  GCLLSCEPGLSCELVNRYIAPSTSCPSHYVGVVLGEPSSQPYPGNVGDVPRFVWNFLADK 544

Query: 164  TSIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            T+IPSKN+SS+C K C+G  E+C++ E DGKGVCVISTTR++PAYSTRLKY+ +
Sbjct: 545  TAIPSKNLSSTCPKGCSGKGEMCVKAETDGKGVCVISTTRYIPAYSTRLKYESD 598


>ref|XP_019223515.1| PREDICTED: nicastrin isoform X3 [Nicotiana attenuata]
          Length = 632

 Score =  589 bits (1519), Expect = 0.0
 Identities = 291/414 (70%), Positives = 351/414 (84%), Gaps = 4/414 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK KK+YT  V++F+LVMQTTKSGT DS+SCL+E TCLPLGGYSVWSALPP+ ISS  KA
Sbjct: 147  EKRKKSYTVDVADFNLVMQTTKSGTRDSESCLREQTCLPLGGYSVWSALPPMAISSSEKA 206

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+L + SMD+ASFFRDK++GA+SPISGLI+LLAVVDALSR+ GL++L+KQLVF V TG
Sbjct: 207  KPVILVVASMDAASFFRDKSIGADSPISGLISLLAVVDALSRVDGLKDLDKQLVFAVFTG 266

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE DQ SD + GLDLA+IETV+EIGSVGK + +  KTFFAH+   T++
Sbjct: 267  EAWGYLGSRRFLLELDQHSDAVSGLDLALIETVLEIGSVGKGFARDDKTFFAHSTSETAT 326

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             NGTL+A+Q A  SL T+S  +  AS SNPG+PPSSLM+FL+K P+ SG+VLEDFD+AF+
Sbjct: 327  -NGTLSAIQDAVGSLKTQSTKISRASKSNPGLPPSSLMSFLKKNPKTSGVVLEDFDTAFT 385

Query: 512  NEFYHSHLDDLS-NINSTSIVAAASLVARSLYILAGGK---DPSAINNIKINSSFVEELL 345
            N+FYHSHLDDL  NINS++IVAAAS+VARSLYILA  K     SA+N I IN+S VEELL
Sbjct: 386  NKFYHSHLDDLPVNINSSAIVAAASVVARSLYILASDKKEIKTSALNTININASLVEELL 445

Query: 344  GCLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADK 165
            GCLL CEPGLSC LV  YI+PST+CPSHYVGV+LGEPSS+PYP   GD+ RFVWNFLADK
Sbjct: 446  GCLLSCEPGLSCELVNRYIAPSTSCPSHYVGVVLGEPSSQPYPGNVGDVPRFVWNFLADK 505

Query: 164  TSIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            T+IPSKN+SS+C K C+G+ E+C++ E DGKGVCVISTTR++PAYSTRLKY+ +
Sbjct: 506  TAIPSKNLSSTCPKGCSGNGEMCVKAETDGKGVCVISTTRYIPAYSTRLKYESD 559


>ref|XP_002264144.1| PREDICTED: nicastrin [Vitis vinifera]
 emb|CBI33472.3| unnamed protein product, partial [Vitis vinifera]
          Length = 671

 Score =  590 bits (1521), Expect = 0.0
 Identities = 289/411 (70%), Positives = 347/411 (84%), Gaps = 3/411 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK KKAYT  V+EFDLVMQTTKSGTHDS+SCLKE TCLPLGGYSVWS+LPPIN+SS  ++
Sbjct: 186  EKNKKAYTADVAEFDLVMQTTKSGTHDSESCLKEETCLPLGGYSVWSSLPPINVSSSDQS 245

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+LT+ SMDSASFFRDK+LGA+SPISGLI+L+A VDALS L GL +L+KQLVF+V TG
Sbjct: 246  KPVILTVASMDSASFFRDKSLGADSPISGLISLMAAVDALSHLDGLNDLSKQLVFLVFTG 305

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE D  SD +KG+D  +IE V+EIGSVGK + QG KTFFAH A  +S 
Sbjct: 306  EAWGYLGSRRFLLELDLHSDFVKGIDSTLIEMVMEIGSVGKGFSQGVKTFFAHVAEVSSV 365

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             N TLNALQ A+DSL +ESIM+ TA++SNPG+PPSSLM+FLRK    SG+VLEDFD+ F+
Sbjct: 366  TNETLNALQQAKDSLKSESIMISTANSSNPGIPPSSLMSFLRKNSSTSGIVLEDFDATFA 425

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAG-GKD--PSAINNIKINSSFVEELLG 342
            N+FYHSHLDDLSN+NS++IVAAASLVAR+LYILA   KD   SA++ I +N+S VE LLG
Sbjct: 426  NQFYHSHLDDLSNVNSSAIVAAASLVARTLYILASDDKDLSTSALSAINVNASLVEALLG 485

Query: 341  CLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKT 162
            CLL+C+PGLSC LVK YI+P T CPS+YVGV+LGEPS+  YP Y  D+SRF+WNFLAD+T
Sbjct: 486  CLLNCDPGLSCDLVKKYIAPRTNCPSNYVGVLLGEPSATLYPGYVSDVSRFIWNFLADRT 545

Query: 161  SIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYD 9
            SIP +N +S+C KDC+ + E+CI EE DGKGVCVISTTR+VPAYSTRL ++
Sbjct: 546  SIPRENATSACPKDCSNEGEVCIGEELDGKGVCVISTTRYVPAYSTRLMFE 596


>emb|CDP01653.1| unnamed protein product [Coffea canephora]
          Length = 618

 Score =  588 bits (1515), Expect = 0.0
 Identities = 288/413 (69%), Positives = 341/413 (82%), Gaps = 3/413 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK+K++YT  V+EF LVMQTTKSGT DS+SCL+E TCLPLGGYSVWSALPPIN S+  K+
Sbjct: 134  EKSKESYTSDVAEFGLVMQTTKSGTRDSESCLREETCLPLGGYSVWSALPPINTSTSQKS 193

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KP++L +TSMDSASFFRDK+LGA+SPISGLIALL  VDALS L GL  L+KQLVFVV TG
Sbjct: 194  KPIILAVTSMDSASFFRDKSLGADSPISGLIALLGAVDALSHLNGLH-LSKQLVFVVFTG 252

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWG+LGSRRF LE DQ SD + GLD  +IETV+EIGSVGKS+ QG K FF HT  A S 
Sbjct: 253  EAWGFLGSRRFLLELDQHSDAVSGLDFTLIETVLEIGSVGKSFDQGVKKFFVHTTEAASI 312

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             N TL+AL+ AQDSL TE++ +  AS SNPG+PPSSLM FLRK P  SG+VLEDFD+AF+
Sbjct: 313  SNQTLDALKRAQDSLKTENVKISVASKSNPGIPPSSLMMFLRKNPDSSGIVLEDFDTAFT 372

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGK---DPSAINNIKINSSFVEELLG 342
            N+FYHSHLD+LSNIN+++I AA+SLVARSLYILA  K   + S +  I +N SF++ELLG
Sbjct: 373  NKFYHSHLDNLSNINASAIAAASSLVARSLYILASEKNEINDSVLTAISVNISFIDELLG 432

Query: 341  CLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKT 162
            CLLDCEPGLSC LV  YISPS  CPSHYVGVI GEPSS PYP Y GD+SRF+WNFLADKT
Sbjct: 433  CLLDCEPGLSCDLVNQYISPSAACPSHYVGVIQGEPSSSPYPGYVGDVSRFLWNFLADKT 492

Query: 161  SIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            S+P++N S++C K C+G  ELCIR E DGKGVCV+S+TR+VPAYSTRLK++ +
Sbjct: 493  SVPTENRSATCPKHCSGTGELCIRGETDGKGVCVVSSTRYVPAYSTRLKFESD 545


>ref|XP_019223513.1| PREDICTED: nicastrin isoform X1 [Nicotiana attenuata]
 gb|OIT34003.1| nicastrin [Nicotiana attenuata]
          Length = 671

 Score =  589 bits (1519), Expect = 0.0
 Identities = 291/414 (70%), Positives = 351/414 (84%), Gaps = 4/414 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK KK+YT  V++F+LVMQTTKSGT DS+SCL+E TCLPLGGYSVWSALPP+ ISS  KA
Sbjct: 186  EKRKKSYTVDVADFNLVMQTTKSGTRDSESCLREQTCLPLGGYSVWSALPPMAISSSEKA 245

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+L + SMD+ASFFRDK++GA+SPISGLI+LLAVVDALSR+ GL++L+KQLVF V TG
Sbjct: 246  KPVILVVASMDAASFFRDKSIGADSPISGLISLLAVVDALSRVDGLKDLDKQLVFAVFTG 305

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE DQ SD + GLDLA+IETV+EIGSVGK + +  KTFFAH+   T++
Sbjct: 306  EAWGYLGSRRFLLELDQHSDAVSGLDLALIETVLEIGSVGKGFARDDKTFFAHSTSETAT 365

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             NGTL+A+Q A  SL T+S  +  AS SNPG+PPSSLM+FL+K P+ SG+VLEDFD+AF+
Sbjct: 366  -NGTLSAIQDAVGSLKTQSTKISRASKSNPGLPPSSLMSFLKKNPKTSGVVLEDFDTAFT 424

Query: 512  NEFYHSHLDDLS-NINSTSIVAAASLVARSLYILAGGK---DPSAINNIKINSSFVEELL 345
            N+FYHSHLDDL  NINS++IVAAAS+VARSLYILA  K     SA+N I IN+S VEELL
Sbjct: 425  NKFYHSHLDDLPVNINSSAIVAAASVVARSLYILASDKKEIKTSALNTININASLVEELL 484

Query: 344  GCLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADK 165
            GCLL CEPGLSC LV  YI+PST+CPSHYVGV+LGEPSS+PYP   GD+ RFVWNFLADK
Sbjct: 485  GCLLSCEPGLSCELVNRYIAPSTSCPSHYVGVVLGEPSSQPYPGNVGDVPRFVWNFLADK 544

Query: 164  TSIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            T+IPSKN+SS+C K C+G+ E+C++ E DGKGVCVISTTR++PAYSTRLKY+ +
Sbjct: 545  TAIPSKNLSSTCPKGCSGNGEMCVKAETDGKGVCVISTTRYIPAYSTRLKYESD 598


>ref|XP_006352434.1| PREDICTED: nicastrin isoform X2 [Solanum tuberosum]
          Length = 670

 Score =  588 bits (1516), Expect = 0.0
 Identities = 291/413 (70%), Positives = 346/413 (83%), Gaps = 3/413 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK KK+ T  V++FDLVMQTTKSGT DS+SCL+E TCLPLGGYSVWSALPPI +SS  KA
Sbjct: 186  EKRKKSSTVDVADFDLVMQTTKSGTRDSESCLREQTCLPLGGYSVWSALPPIIVSSSKKA 245

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+LT+ SMD+AS FRD ++GA+SPISGLI+LLAVVDALSR+ GL +L+KQLVF V TG
Sbjct: 246  KPVILTVASMDAASLFRDVSIGADSPISGLISLLAVVDALSRVDGLGDLDKQLVFAVFTG 305

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE DQ SD + GLDLA+IETV+EIGSVGK + Q   TFFAH+    S+
Sbjct: 306  EAWGYLGSRRFLLELDQHSDAVSGLDLALIETVLEIGSVGKGFTQDDNTFFAHST-TESA 364

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             NGTL+AL+ A  SL T+SI +  AS SNPG+PPSSLM+FL+K P+ SG+VLEDFD+AF+
Sbjct: 365  TNGTLSALKDALGSLKTQSIKISRASKSNPGLPPSSLMSFLKKNPETSGVVLEDFDAAFT 424

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGKDP---SAINNIKINSSFVEELLG 342
            N+FYHSHLDDLSNINS++IVAAAS+VARSLYILA  K     S +N I IN+S VEELLG
Sbjct: 425  NKFYHSHLDDLSNINSSAIVAAASIVARSLYILASDKKEIKNSVLNTININASLVEELLG 484

Query: 341  CLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKT 162
            CLL CEPG +C LV HYI+PST CPSHYVGV++GEPSS+PY    GD+SRFVWNFLADKT
Sbjct: 485  CLLSCEPGYTCELVNHYIAPSTRCPSHYVGVVMGEPSSQPYLGNVGDVSRFVWNFLADKT 544

Query: 161  SIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            +IPSKN+SS+C K C+G+ E+C++ E DGKGVCVISTTR+VPAYSTRLKY+ E
Sbjct: 545  AIPSKNMSSTCPKGCSGNGEMCVKAETDGKGVCVISTTRYVPAYSTRLKYESE 597


>ref|XP_016488669.1| PREDICTED: nicastrin-like isoform X2 [Nicotiana tabacum]
          Length = 670

 Score =  588 bits (1515), Expect = 0.0
 Identities = 292/413 (70%), Positives = 346/413 (83%), Gaps = 3/413 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK KK+YT  V++F+LVMQTTKSGT DS+SCL+E TCLPLGGYSVWSALPPI ISS  KA
Sbjct: 186  EKRKKSYTVDVADFNLVMQTTKSGTRDSESCLREQTCLPLGGYSVWSALPPIAISSSEKA 245

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+L + SMD+ASFFRDK++GA+SPISGLI+LLAVVDALSR  G++ L+KQLVF V TG
Sbjct: 246  KPVILIVASMDTASFFRDKSIGADSPISGLISLLAVVDALSRADGVKGLDKQLVFAVFTG 305

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE DQ SD + GLDLA+IETV+EIGSVGK + Q  KTFFAH+   T++
Sbjct: 306  EAWGYLGSRRFLLELDQHSDAVSGLDLALIETVLEIGSVGKGFAQDDKTFFAHSTSETAT 365

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             NG L+A+Q A  SL  +SI V  AS SNPG+PPSSLM+FL+K P+ SG+VLEDFD+AF+
Sbjct: 366  -NGMLSAIQDALGSLNNQSIKVSRASKSNPGLPPSSLMSFLKKNPKTSGVVLEDFDTAFT 424

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGK---DPSAINNIKINSSFVEELLG 342
            N+FYHSHLDDL NINS++IVAAAS+VARSLYILA  K     SA+N I IN+S VEELLG
Sbjct: 425  NKFYHSHLDDLLNINSSAIVAAASVVARSLYILASDKKEIKTSALNTININASLVEELLG 484

Query: 341  CLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKT 162
            CLL CEPGLSC LV  YI+PS +CPSHYVGV+LGEPSS+PYP   GD+ RFVWNFLADKT
Sbjct: 485  CLLSCEPGLSCELVNRYIAPSASCPSHYVGVVLGEPSSQPYPGNVGDVPRFVWNFLADKT 544

Query: 161  SIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            +IPSKN+SS+C K C+G  E+C++ E DGKGVCVISTTR+VPAYSTRLKY+ +
Sbjct: 545  AIPSKNMSSACPKGCSGTGEVCVKAETDGKGVCVISTTRYVPAYSTRLKYESD 597


>ref|XP_009626875.1| PREDICTED: nicastrin isoform X2 [Nicotiana tomentosiformis]
          Length = 670

 Score =  588 bits (1515), Expect = 0.0
 Identities = 292/413 (70%), Positives = 346/413 (83%), Gaps = 3/413 (0%)
 Frame = -3

Query: 1232 EKTKKAYTKVVSEFDLVMQTTKSGTHDSDSCLKEGTCLPLGGYSVWSALPPINISSPLKA 1053
            EK KK+YT  V++F+LVMQTTKSGT DS+SCL+E TCLPLGGYSVWSALPPI ISS  KA
Sbjct: 186  EKRKKSYTVDVADFNLVMQTTKSGTRDSESCLREQTCLPLGGYSVWSALPPIAISSSEKA 245

Query: 1052 KPVVLTMTSMDSASFFRDKNLGAESPISGLIALLAVVDALSRLGGLEELNKQLVFVVLTG 873
            KPV+L + SMD+ASFFRDK++GA+SPISGLI+LLAVVDALSR  G++ L+KQLVF V TG
Sbjct: 246  KPVILIVASMDTASFFRDKSIGADSPISGLISLLAVVDALSRADGVKGLDKQLVFAVFTG 305

Query: 872  EAWGYLGSRRFFLESDQQSDTMKGLDLAMIETVVEIGSVGKSYPQGTKTFFAHTAGATSS 693
            EAWGYLGSRRF LE DQ SD + GLDLA+IETV+EIGSVGK + Q  KTFFAH+   T++
Sbjct: 306  EAWGYLGSRRFLLELDQHSDAVSGLDLALIETVLEIGSVGKGFAQDDKTFFAHSTSETAT 365

Query: 692  VNGTLNALQHAQDSLITESIMVKTASTSNPGVPPSSLMTFLRKKPQISGLVLEDFDSAFS 513
             NG L+A+Q A  SL  +SI V  AS SNPG+PPSSLM+FL+K P+ SG+VLEDFD+AF+
Sbjct: 366  -NGMLSAIQDALGSLNNQSIKVSRASKSNPGLPPSSLMSFLKKNPKTSGVVLEDFDTAFT 424

Query: 512  NEFYHSHLDDLSNINSTSIVAAASLVARSLYILAGGK---DPSAINNIKINSSFVEELLG 342
            N+FYHSHLDDL NINS++IVAAAS+VARSLYILA  K     SA+N I IN+S VEELLG
Sbjct: 425  NKFYHSHLDDLLNINSSAIVAAASVVARSLYILASDKKEIKTSALNTININASLVEELLG 484

Query: 341  CLLDCEPGLSCGLVKHYISPSTTCPSHYVGVILGEPSSKPYPAYAGDISRFVWNFLADKT 162
            CLL CEPGLSC LV  YI+PS +CPSHYVGV+LGEPSS+PYP   GD+ RFVWNFLADKT
Sbjct: 485  CLLSCEPGLSCELVNRYIAPSASCPSHYVGVVLGEPSSQPYPGNVGDVPRFVWNFLADKT 544

Query: 161  SIPSKNVSSSCSKDCNGDNELCIREEADGKGVCVISTTRFVPAYSTRLKYDDE 3
            +IPSKN+SS+C K C+G  E+C++ E DGKGVCVISTTR+VPAYSTRLKY+ +
Sbjct: 545  AIPSKNMSSACPKGCSGTGEVCVKAETDGKGVCVISTTRYVPAYSTRLKYESD 597