BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00011263
         (2355 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011082531.1| putative E3 ubiquitin-protein ligase RF4 [Se...   738   0.0  
gb|KZV43102.1| hypothetical protein F511_04494 [Dorcoceras hygro...   607   0.0  
ref|XP_022854546.1| putative E3 ubiquitin-protein ligase RF298 [...   597   0.0  
ref|XP_009789682.1| PREDICTED: putative E3 ubiquitin-protein lig...   496   e-161
ref|XP_009622635.1| PREDICTED: putative E3 ubiquitin-protein lig...   496   e-161
ref|XP_009622634.1| PREDICTED: putative E3 ubiquitin-protein lig...   496   e-160
ref|XP_016482982.1| PREDICTED: putative E3 ubiquitin-protein lig...   495   e-160
ref|XP_016482981.1| PREDICTED: putative E3 ubiquitin-protein lig...   495   e-160
ref|XP_019234828.1| PREDICTED: putative E3 ubiquitin-protein lig...   492   e-159
ref|XP_006355787.1| PREDICTED: putative E3 ubiquitin-protein lig...   488   e-157
ref|XP_015078209.1| PREDICTED: putative E3 ubiquitin-protein lig...   481   e-154
ref|XP_010321841.1| PREDICTED: putative E3 ubiquitin-protein lig...   478   e-153
ref|XP_015078210.1| PREDICTED: putative E3 ubiquitin-protein lig...   476   e-153
ref|XP_019228345.1| PREDICTED: putative E3 ubiquitin-protein lig...   471   e-151
ref|XP_009614905.1| PREDICTED: putative E3 ubiquitin-protein lig...   471   e-151
ref|XP_009798672.1| PREDICTED: putative E3 ubiquitin-protein lig...   461   e-147
ref|XP_016463481.1| PREDICTED: putative E3 ubiquitin-protein lig...   460   e-147
ref|XP_015168014.1| PREDICTED: putative E3 ubiquitin-protein lig...   444   e-140
ref|XP_016565617.1| PREDICTED: putative E3 ubiquitin-protein lig...   389   e-119
gb|PIN13412.1| putative E3 ubiquitin ligase [Handroanthus impeti...   367   e-114

>ref|XP_011082531.1| putative E3 ubiquitin-protein ligase RF4 [Sesamum indicum]
 ref|XP_011082532.1| putative E3 ubiquitin-protein ligase RF4 [Sesamum indicum]
 ref|XP_020550376.1| putative E3 ubiquitin-protein ligase RF4 [Sesamum indicum]
          Length = 836

 Score =  738 bits (1904), Expect = 0.0
 Identities = 418/714 (58%), Positives = 494/714 (69%), Gaps = 31/714 (4%)
 Frame = -2

Query: 2051 MGESGSNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEK 1875
            M +SGS+AD S+EGLS+VLVNEKG RNKRK+LSDL +DIP +VS++SLTEFPRYEMLEEK
Sbjct: 1    MADSGSSADGSSEGLSSVLVNEKGSRNKRKYLSDLSVDIP-EVSSMSLTEFPRYEMLEEK 59

Query: 1874 FRNVLDELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKI 1695
            +RN L+ELGSMMERS+  +                PI CQLEELL+N L+ TFC+A+KKI
Sbjct: 60   YRNALNELGSMMERSDDTLEEHEVEEYLKADWDD-PICCQLEELLTNNLFTTFCSAVKKI 118

Query: 1694 VESGYNEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLV 1515
            VESGY EEVAEWAVLNSS+FHG KDA+SNVVD ALALLKKEK++NTPKH  VFEGLQ LV
Sbjct: 119  VESGYTEEVAEWAVLNSSLFHGSKDAVSNVVDSALALLKKEKELNTPKH-PVFEGLQCLV 177

Query: 1514 DYTLLEMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPAL 1335
            DYTLLEMIHV+REV+PTLT+ EAMWCLL++DLNLVNACV EGG   GS  QEA   SP L
Sbjct: 178  DYTLLEMIHVLREVRPTLTVAEAMWCLLITDLNLVNACVVEGGPLGGSSGQEAQRGSPRL 237

Query: 1334 XXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPNNKPVSGESA---KE 1164
                          +NKLDN KQ  PRAQ  + E PV+GPA Q  +++    E A   KE
Sbjct: 238  SQSKSETSSTSQADTNKLDNPKQTMPRAQSSQHEFPVSGPAPQPLSSRAAPDELASTGKE 297

Query: 1163 SSFSLQESKG-----------------ATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKN 1035
             S +LQE+KG                  TDE               RD+LRQK F FEKN
Sbjct: 298  ISLALQEAKGKLSVIGREHIQTSTQAMVTDEKSGGSRKGPSTNSK-RDMLRQKTFHFEKN 356

Query: 1034 YKGRLSKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXXX 855
            YKGR+SKGAFK+KVAAWGSMVLDK          VVMKG  +KLTT A T          
Sbjct: 357  YKGRMSKGAFKAKVAAWGSMVLDKSLKSQSGSSGVVMKG--TKLTTSAGTNRSLVEGSQP 414

Query: 854  XXXV----------KDPVFALPAVNSKSPASTVPDSDSCPNVEINVSDPPKPTNYFSSIP 705
                           DP F LP  NS+SP S+VPD+  C   EI VSDPPK T+Y++ IP
Sbjct: 415  PSSNLQSAVPSGVLSDPEFPLPGFNSQSPESSVPDTAPCAKGEITVSDPPKITDYYALIP 474

Query: 704  FDEILQKYVPQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKML 525
            FDE LQK++PQDDK+ETILMLVP K+ALEKEL+GWTDWANEKVMQAAR+LGKDQGELKML
Sbjct: 475  FDETLQKHMPQDDKEETILMLVPLKQALEKELQGWTDWANEKVMQAARRLGKDQGELKML 534

Query: 524  RQXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEM 345
            RQ           KQ+L+E+ +KRL EME+AL+N  GQIE+ANC VRRLEEENSLLKKEM
Sbjct: 535  RQEKEETEKFKKEKQSLEESTMKRLSEMEYALSNATGQIEVANCTVRRLEEENSLLKKEM 594

Query: 344  EAATFQARASADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAK 165
            E A  QA  SA  LQ+A+++EQE LKK+QSW+AEK LV+E++T+LK Q+AELEN LEKAK
Sbjct: 595  EDAKLQALKSATNLQEALMREQEALKKLQSWDAEKGLVLEQLTDLKSQIAELENILEKAK 654

Query: 164  DRHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
             R NQF                +ID++R K  E++A LKVEAD +KQ A+ + Q
Sbjct: 655  GRQNQFKVLLKHEEKEKLKVVNQIDTVRRKIAEEDALLKVEADRVKQTAEMSMQ 708


>gb|KZV43102.1| hypothetical protein F511_04494 [Dorcoceras hygrometricum]
          Length = 838

 Score =  607 bits (1564), Expect = 0.0
 Identities = 361/713 (50%), Positives = 450/713 (63%), Gaps = 30/713 (4%)
 Frame = -2

Query: 2051 MGESGSNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEK 1875
            MGE G   +  NEG S  LV EKG RNKRKFL +L +DIPVDV+TLS TEFPR++ML+EK
Sbjct: 1    MGEHGK-IEGINEGPSTGLVKEKGSRNKRKFLPNLSIDIPVDVTTLSRTEFPRHDMLQEK 59

Query: 1874 FRNVLDELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKI 1695
            FR  L  LGS++ RS  +                +PI CQLEELL+N L ATF +A+K++
Sbjct: 60   FRTALSNLGSLIARSA-DTSEEQEFEEFQQANWENPIACQLEELLTNNLLATFRSAIKQV 118

Query: 1694 VESGYNEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLV 1515
             E GY EE AEWAVLNS ++HG KDA++NVVDGALALL  EK+ +T  +H +F GLQ+LV
Sbjct: 119  AECGYTEEAAEWAVLNSGLYHGIKDAVTNVVDGALALLS-EKEFST-SNHPLFGGLQNLV 176

Query: 1514 DYTLLEMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPA- 1338
            DYT+LEMIHV+REV+P LTITEAMW LL+SD+NLVNACV EG  +  SC  E+  ES   
Sbjct: 177  DYTILEMIHVLREVRPALTITEAMWYLLISDMNLVNACVMEGVTSGSSCVPESPVESSVA 236

Query: 1337 -LXXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPNNKPVSGESA--- 1170
             +              S   ++ +Q  P AQ  +  +P++ P  Q+PN+ P + E A   
Sbjct: 237  TISQSKDGGSVNSHLCSKTCNDPRQLMPHAQSSQFGSPISVPVHQIPNSNPHAEEIAGVV 296

Query: 1169 KESSFSLQESKGA----------------TDEXXXXXXXXXXXXXXKRDLLRQKAFQFEK 1038
            KE S + QE KG                 T +              KRDLLRQK FQFEK
Sbjct: 297  KEGSVAPQEVKGKFSAITREKIQTSSQSMTVDERSGGSKKGPSGSSKRDLLRQKTFQFEK 356

Query: 1037 NYKGRLSKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXX 858
            +YKG   +G+ K+KVAAWGSMVLDK         SV  KG YSKLT   ++         
Sbjct: 357  SYKGHDVEGSLKAKVAAWGSMVLDKSSKSQSGSSSVATKGTYSKLTGIIDSSAEGKHHLS 416

Query: 857  XXXXV--------KDPVFALPAVNSKSPASTVPDSDSCPNVEINVSDPPKPTNYFSSIPF 702
                         KDPVFALPAV SKS  ST+PD  S  N + +VS   K  +YFS IP+
Sbjct: 417  SNSQHDGPGVLPVKDPVFALPAVISKSSVSTIPDPISLSNGDPHVSGSLKSADYFSGIPY 476

Query: 701  DEILQKYVPQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLR 522
            DE LQKY+PQDD+DETIL+L PHK ALE+EL+GWT WAN+KVMQAAR+LG DQGELK+LR
Sbjct: 477  DETLQKYLPQDDRDETILILAPHKRALERELQGWTHWANDKVMQAARRLGSDQGELKLLR 536

Query: 521  QXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEME 342
            Q           +Q+ +EN +KRL EME+AL+N  GQIE+AN AVRRL EENSLLKK+ME
Sbjct: 537  QEKEETEKLNKERQSSEENTLKRLSEMENALSNASGQIEMANSAVRRLGEENSLLKKKME 596

Query: 341  AATFQARASADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKD 162
             A  QA  +A  LQ+AV +EQ+ LKK Q W AEK L+ E++T+LKRQ+AEL N+LEKAK 
Sbjct: 597  DAKSQALRAAFNLQEAVQREQDVLKKSQLWNAEKGLMQEQLTDLKRQVAELNNQLEKAKV 656

Query: 161  RHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
            + +QF                 IDSLR K+E+D+A  K E D IKQ+ + N Q
Sbjct: 657  QVDQFKVLMKQEEREKIKALNCIDSLRQKKEDDDALTKEEEDKIKQMGEQNLQ 709


>ref|XP_022854546.1| putative E3 ubiquitin-protein ligase RF298 [Olea europaea var.
            sylvestris]
          Length = 809

 Score =  597 bits (1540), Expect = 0.0
 Identities = 351/705 (49%), Positives = 443/705 (62%), Gaps = 22/705 (3%)
 Frame = -2

Query: 2051 MGESGSNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEK 1875
            MG +G N   + EG S V   EKG RNKRK LSDL LD P+DVST SLTEFPRYE+LEEK
Sbjct: 1    MGVNG-NVHGNGEGSSDVSATEKGSRNKRKLLSDLVLDNPIDVSTFSLTEFPRYELLEEK 59

Query: 1874 FRNVLDELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKI 1695
            FRN L ELGS++ RSE  I               DP TCQLE++L++ L ATF +A+KKI
Sbjct: 60   FRNALSELGSLVSRSE-KIVAEPEVEEFHPSDWDDPFTCQLEKILTSNLLATFRSAVKKI 118

Query: 1694 VESGYNEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLV 1515
            VE GYNEE AEW +LN S++HGRKDA+SNVVDG LALLK+EK+ N P+   VF+ LQSL 
Sbjct: 119  VECGYNEETAEWVILNCSIYHGRKDAVSNVVDGVLALLKREKEFNFPRL-PVFQDLQSLA 177

Query: 1514 DYTLLEMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEG---GATSGSCEQEASG-- 1350
            +YT+LEMIHVVREVKP+LTI EAMW LLL DLNL+ AC  E    G   G C  E  G  
Sbjct: 178  EYTMLEMIHVVREVKPSLTIVEAMWRLLLCDLNLLYACAVEQDSLGYIGGICGPEVLGGN 237

Query: 1349 ESPALXXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPNNKPVSGESA 1170
               +               +NK  N KQ     Q  +P+   AG   QLPN+K       
Sbjct: 238  SESSFPESKPKLSDTSSLITNKPHNPKQS---IQSSQPKNLTAGAVSQLPNSKISRSRPK 294

Query: 1169 KESSFSLQESKGATD----------------EXXXXXXXXXXXXXXKRDLLRQKAFQFEK 1038
            K SS S Q+++G                   +              KRD+LRQK+F FEK
Sbjct: 295  KGSSSSFQDARGKFSGITKEHIRTSSQATVLDEKSGGSRTGLSGNSKRDMLRQKSFNFEK 354

Query: 1037 NYKGRLSKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXX 858
            +YKG +SKGAFK+KVAAWG+MVLDK         S+ MK +    T P +          
Sbjct: 355  SYKGCVSKGAFKAKVAAWGNMVLDKTLKSQSDSSSIAMKSHPPSCTLPVKDTRTLPI--- 411

Query: 857  XXXXVKDPVFALPAVNSKSPASTVPDSDSCPNVEINVSDPPKPTNYFSSIPFDEILQKYV 678
                 KD   ALPA+ +KSPAS+  +S+   N +I+ S P   T+Y+++IP+DE LQ Y+
Sbjct: 412  -----KDNDHALPALYAKSPASS--ESELSSNTKIDKSGPSSATDYYAAIPYDESLQNYI 464

Query: 677  PQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLRQXXXXXXX 498
            PQDDK+ET+L+LVPHK+ALEKEL+GWTDWANEKVMQAAR+L KD+GELK+LRQ       
Sbjct: 465  PQDDKEETLLILVPHKQALEKELQGWTDWANEKVMQAARRLRKDRGELKILRQEKEETEK 524

Query: 497  XXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEMEAATFQARA 318
                KQ L+EN +KRL EME+AL+N  GQIE+AN AV+RLEEENS+L+KEMEAA  QA  
Sbjct: 525  FKEEKQILEENTMKRLSEMEYALSNATGQIEVANSAVQRLEEENSVLRKEMEAAKLQALG 584

Query: 317  SADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKDRHNQFXXX 138
            SA  LQ+A+++EQE LKK +SW+ EK L +E+ T LKR  A+L+N+L+K K+R + F   
Sbjct: 585  SATNLQEAMLREQEALKKSKSWDMEKDLFLEQRTKLKRLAADLQNQLKKTKERKDNFKIL 644

Query: 137  XXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
                          IDS + KREE++A  K E D +KQ+A+ + Q
Sbjct: 645  WRQEEQEKRTAVSHIDSFKKKREEEDALAKAEEDDLKQVAELDVQ 689


>ref|XP_009789682.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            sylvestris]
          Length = 813

 Score =  496 bits (1278), Expect = e-161
 Identities = 302/694 (43%), Positives = 405/694 (58%), Gaps = 16/694 (2%)
 Frame = -2

Query: 2036 SNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRNVL 1860
            +N + ++E   A+   EKG RNKRKFLS+ PLDIPVD   LSLTEFPRYE+LEEK ++  
Sbjct: 13   NNGECADEKSLAISAQEKGSRNKRKFLSESPLDIPVDAPALSLTEFPRYELLEEKLQSTP 72

Query: 1859 DELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVESGY 1680
            + +GS+  R   +                D I CQL ELL + L ATF +A+K+IVE GY
Sbjct: 73   NGVGSLEGRCHQS-NEKQGVETLQHADWDDTIACQLMELLFHNLSATFQSAIKRIVECGY 131

Query: 1679 NEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEK--DVNTPKHHSVFEGLQSLVDYT 1506
            +EE+AEW +L S ++HG KDA+S +VDGALALL +EK  D +TP    +FE L SLV+YT
Sbjct: 132  SEEIAEWVILRSGLYHGSKDAVSTIVDGALALLSREKELDSSTPL---IFEDLNSLVEYT 188

Query: 1505 LLEMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPALXXX 1326
            +LEMI V+REVKP  T+ EAMWCLL+ DLNL++AC  EG      C  E+  ES      
Sbjct: 189  MLEMICVLREVKPAFTVAEAMWCLLICDLNLLHACAIEGDLLGELCSLESPRESSCGSKL 248

Query: 1325 XXXXXXXXXXXSN--KLDNLKQRDPRAQMFRPETPVAGPAQQLPNN-----KPVSGESAK 1167
                       SN  KL   K   P AQ    E P A    QL  +       V     +
Sbjct: 249  AQTKEASELIQSNMDKLQPPKPSMPIAQSLHSEVPCADVIAQLQESIYSHLHEVGITGNQ 308

Query: 1166 ESSFSLQE-----SKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKNYKGRLSKGAFK 1002
             SS  ++E     SK A  E               +D+LRQK+F FEK+YKGR+ KG+FK
Sbjct: 309  SSSGLIRENILSLSKAAILEDIAGAAKRGSSK---KDILRQKSFHFEKSYKGRMGKGSFK 365

Query: 1001 SKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXXXXXXVKDPVFAL 822
            +K+  WGSMVLDK           VMK  YSK+TT  +T                     
Sbjct: 366  AKLTTWGSMVLDKTLNSESGSSGFVMKSTYSKVTTSLKTNGPLAEGSSHSSSTS------ 419

Query: 821  PAVNSKSPASTV-PDSDSCPNVEINVSDPPKPTNYFSSIPFDEILQKYVPQDDKDETILM 645
            P++   S  S+V P  D+   +    ++ P   +Y++ IP+DE L K+VPQ++KDETIL+
Sbjct: 420  PSIAPSSETSSVQPAQDTVCALPAVNTNTPAVVDYYAGIPYDESLGKHVPQNEKDETILL 479

Query: 644  LVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLRQXXXXXXXXXXXKQTLQEN 465
            L+   + L+KEL+GWTDWANEKVMQAAR+LGKD+ ELKMLRQ           KQ ++EN
Sbjct: 480  LISRMQTLQKELQGWTDWANEKVMQAARRLGKDKEELKMLRQEKEEADKSQKEKQMVEEN 539

Query: 464  NIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEMEAATFQARASADKLQDAVVK 285
              KRL EM++AL+N  GQIE+AN ++RRLE EN++LKKEMEAA   A ASA     AV +
Sbjct: 540  AAKRLSEMDYALSNASGQIEMANSSLRRLEVENAVLKKEMEAAKLAAVASATNFHQAVAR 599

Query: 284  EQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKDRHNQFXXXXXXXXXXXXXX 105
            EQE LKK Q+WE EK  + +  + LK+++A LE  LE+AK   NQF              
Sbjct: 600  EQEILKKCQAWEIEKGSLQDDFSTLKQKVAHLEQELERAKKHQNQFKALSEQEEREKQRV 659

Query: 104  XKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
             ++ DS++ +RE+     K+E D+++++A++N Q
Sbjct: 660  LQQADSVKAEREQRGVESKMEEDNMREMAESNMQ 693


>ref|XP_009622635.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Nicotiana tomentosiformis]
 ref|XP_009622636.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Nicotiana tomentosiformis]
 ref|XP_018632442.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Nicotiana tomentosiformis]
          Length = 814

 Score =  496 bits (1277), Expect = e-161
 Identities = 303/700 (43%), Positives = 406/700 (58%), Gaps = 20/700 (2%)
 Frame = -2

Query: 2042 SGSNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRN 1866
            SG  AD  +  +SA    EKG RNKRKFLS+ PLDIPVD   LSLTEFPRYE+LEEK ++
Sbjct: 14   SGECADEKSLAISA---QEKGSRNKRKFLSESPLDIPVDAPALSLTEFPRYELLEEKHQS 70

Query: 1865 VLDELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVES 1686
              + +GS+  R  +                 D I CQL ELL + L ATF +A+K+IVE 
Sbjct: 71   TPNGVGSLEGRC-HKSNEKQGVETLQHADWDDTIACQLMELLFHNLSATFQSAIKRIVEC 129

Query: 1685 GYNEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEK--DVNTPKHHSVFEGLQSLVD 1512
            GY+EE+AEW +L S ++HG KDA+S +VDGALALL +EK  D +TP    +FE L SLV+
Sbjct: 130  GYSEEIAEWVILRSGLYHGSKDAVSTIVDGALALLSREKELDSSTPL---IFEDLNSLVE 186

Query: 1511 YTLLEMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPA-- 1338
            YT+LEMI V+REVKP  T+ EAMWCLL+ DLNL++AC  EG      C  E+  ES +  
Sbjct: 187  YTMLEMICVLREVKPAFTVAEAMWCLLICDLNLLHACAIEGDLLGELCGLESPRESSSGS 246

Query: 1337 -LXXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPN------------ 1197
                             NKL + K   P AQ    E P A    QL              
Sbjct: 247  KFAQTKEASEVIQSNMDNKLQSSKPSMPIAQSLHSEVPCADVIAQLQESIYSHLHEVGIT 306

Query: 1196 -NKPVSGESAKESSFSLQESKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKNYKGRL 1020
             N+  SG   +E+  SL ++    D+               +D+LRQK+F FEK+YKGR+
Sbjct: 307  GNESSSG-LIRENILSLSKAAILEDKSGTAKRGSSK-----KDILRQKSFHFEKSYKGRM 360

Query: 1019 SKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXXXXXXVK 840
             KG+FK+K+  WGSMVLDK           VMK  YSK+T   +T               
Sbjct: 361  CKGSFKAKLTTWGSMVLDKTLNSESGSSGFVMKSTYSKVTASLKTNGPLAEGSSHSSSTS 420

Query: 839  DPVFALPAVNSKSPASTV-PDSDSCPNVEINVSDPPKPTNYFSSIPFDEILQKYVPQDDK 663
                  P++   S  S+V P  D+   +    ++     +Y++ IP+DE L K+VPQ++K
Sbjct: 421  ------PSIAPSSETSSVQPTQDTICALPAVNTNTLAVVDYYAGIPYDESLGKHVPQNEK 474

Query: 662  DETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLRQXXXXXXXXXXXK 483
            DETIL+L+   + L+KEL+GWTDWANEKVMQAAR+LGKD+GELKMLRQ           K
Sbjct: 475  DETILLLISRMQTLQKELQGWTDWANEKVMQAARRLGKDKGELKMLRQEKEEAEKSQKEK 534

Query: 482  QTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEMEAATFQARASADKL 303
            Q ++EN  KRL EME+AL+N  GQI++AN ++RRLE EN++LKKEMEAA   A ASA   
Sbjct: 535  QMVEENTTKRLSEMEYALSNASGQIKMANSSLRRLEVENAVLKKEMEAAKLAAVASATNF 594

Query: 302  QDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKDRHNQFXXXXXXXX 123
              AV +EQE LKK Q+WE EK  + +  + LK++ A LE  LE+AK R NQF        
Sbjct: 595  HQAVAREQEILKKCQAWEIEKGSLQDDFSTLKQKAAHLEQELERAKKRQNQFKALSEQEE 654

Query: 122  XXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
                   ++ DS++ +RE+     K+E D+++++A++N Q
Sbjct: 655  RGKQRVLQQADSVKAEREQWGVESKMEEDNMREMAESNMQ 694


>ref|XP_009622634.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Nicotiana tomentosiformis]
          Length = 837

 Score =  496 bits (1277), Expect = e-160
 Identities = 303/700 (43%), Positives = 406/700 (58%), Gaps = 20/700 (2%)
 Frame = -2

Query: 2042 SGSNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRN 1866
            SG  AD  +  +SA    EKG RNKRKFLS+ PLDIPVD   LSLTEFPRYE+LEEK ++
Sbjct: 37   SGECADEKSLAISA---QEKGSRNKRKFLSESPLDIPVDAPALSLTEFPRYELLEEKHQS 93

Query: 1865 VLDELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVES 1686
              + +GS+  R  +                 D I CQL ELL + L ATF +A+K+IVE 
Sbjct: 94   TPNGVGSLEGRC-HKSNEKQGVETLQHADWDDTIACQLMELLFHNLSATFQSAIKRIVEC 152

Query: 1685 GYNEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEK--DVNTPKHHSVFEGLQSLVD 1512
            GY+EE+AEW +L S ++HG KDA+S +VDGALALL +EK  D +TP    +FE L SLV+
Sbjct: 153  GYSEEIAEWVILRSGLYHGSKDAVSTIVDGALALLSREKELDSSTPL---IFEDLNSLVE 209

Query: 1511 YTLLEMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPA-- 1338
            YT+LEMI V+REVKP  T+ EAMWCLL+ DLNL++AC  EG      C  E+  ES +  
Sbjct: 210  YTMLEMICVLREVKPAFTVAEAMWCLLICDLNLLHACAIEGDLLGELCGLESPRESSSGS 269

Query: 1337 -LXXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPN------------ 1197
                             NKL + K   P AQ    E P A    QL              
Sbjct: 270  KFAQTKEASEVIQSNMDNKLQSSKPSMPIAQSLHSEVPCADVIAQLQESIYSHLHEVGIT 329

Query: 1196 -NKPVSGESAKESSFSLQESKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKNYKGRL 1020
             N+  SG   +E+  SL ++    D+               +D+LRQK+F FEK+YKGR+
Sbjct: 330  GNESSSG-LIRENILSLSKAAILEDKSGTAKRGSSK-----KDILRQKSFHFEKSYKGRM 383

Query: 1019 SKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXXXXXXVK 840
             KG+FK+K+  WGSMVLDK           VMK  YSK+T   +T               
Sbjct: 384  CKGSFKAKLTTWGSMVLDKTLNSESGSSGFVMKSTYSKVTASLKTNGPLAEGSSHSSSTS 443

Query: 839  DPVFALPAVNSKSPASTV-PDSDSCPNVEINVSDPPKPTNYFSSIPFDEILQKYVPQDDK 663
                  P++   S  S+V P  D+   +    ++     +Y++ IP+DE L K+VPQ++K
Sbjct: 444  ------PSIAPSSETSSVQPTQDTICALPAVNTNTLAVVDYYAGIPYDESLGKHVPQNEK 497

Query: 662  DETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLRQXXXXXXXXXXXK 483
            DETIL+L+   + L+KEL+GWTDWANEKVMQAAR+LGKD+GELKMLRQ           K
Sbjct: 498  DETILLLISRMQTLQKELQGWTDWANEKVMQAARRLGKDKGELKMLRQEKEEAEKSQKEK 557

Query: 482  QTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEMEAATFQARASADKL 303
            Q ++EN  KRL EME+AL+N  GQI++AN ++RRLE EN++LKKEMEAA   A ASA   
Sbjct: 558  QMVEENTTKRLSEMEYALSNASGQIKMANSSLRRLEVENAVLKKEMEAAKLAAVASATNF 617

Query: 302  QDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKDRHNQFXXXXXXXX 123
              AV +EQE LKK Q+WE EK  + +  + LK++ A LE  LE+AK R NQF        
Sbjct: 618  HQAVAREQEILKKCQAWEIEKGSLQDDFSTLKQKAAHLEQELERAKKRQNQFKALSEQEE 677

Query: 122  XXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
                   ++ DS++ +RE+     K+E D+++++A++N Q
Sbjct: 678  RGKQRVLQQADSVKAEREQWGVESKMEEDNMREMAESNMQ 717


>ref|XP_016482982.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Nicotiana tabacum]
 ref|XP_016482983.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Nicotiana tabacum]
 ref|XP_016482984.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Nicotiana tabacum]
          Length = 814

 Score =  495 bits (1274), Expect = e-160
 Identities = 302/700 (43%), Positives = 406/700 (58%), Gaps = 20/700 (2%)
 Frame = -2

Query: 2042 SGSNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRN 1866
            SG  AD  +  +SA    EKG RNKRKFLS+ PLDIPVD   LSLTEFPRYE+LEEK ++
Sbjct: 14   SGECADEKSLAISA---QEKGSRNKRKFLSESPLDIPVDAPALSLTEFPRYELLEEKHQS 70

Query: 1865 VLDELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVES 1686
              + +GS+  R  +                 D I CQL ELL + L ATF +A+K+IVE 
Sbjct: 71   TPNGVGSLEGRC-HKSNEKQGVETLQHADWDDTIACQLMELLFHNLSATFQSAIKRIVEC 129

Query: 1685 GYNEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEK--DVNTPKHHSVFEGLQSLVD 1512
            GY+EE+AEW +L S ++HG KDA+S +VDGALALL +EK  D +TP    +FE L SLV+
Sbjct: 130  GYSEEIAEWVILRSGLYHGSKDAVSTIVDGALALLSREKELDSSTPL---IFEDLNSLVE 186

Query: 1511 YTLLEMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPA-- 1338
            YT+LEMI V+REVKP  T+ EAMWCLL+ DLNL++AC  EG      C  E+  ES +  
Sbjct: 187  YTMLEMICVLREVKPAFTVAEAMWCLLICDLNLLHACAIEGDLLGELCGLESPRESSSGS 246

Query: 1337 -LXXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPN------------ 1197
                             NKL + K   P AQ    E P A    QL              
Sbjct: 247  KFAQTKEASEVIQSNMDNKLQSSKPSMPIAQSLHSEVPCADVIAQLQESIYSHLHEVGIT 306

Query: 1196 -NKPVSGESAKESSFSLQESKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKNYKGRL 1020
             N+  SG   +E+  SL ++    D+               +D+LRQK+F FEK+YKGR+
Sbjct: 307  GNESSSG-LIRENILSLSKAAILEDKSGTAKRGSSK-----KDILRQKSFHFEKSYKGRM 360

Query: 1019 SKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXXXXXXVK 840
             KG+FK+K+  WGSMVLDK           VMK  YSK+T   +T               
Sbjct: 361  GKGSFKAKLTTWGSMVLDKTLNSESGSSGFVMKSTYSKVTASLKTNGPLAEGSSHSSSTS 420

Query: 839  DPVFALPAVNSKSPASTV-PDSDSCPNVEINVSDPPKPTNYFSSIPFDEILQKYVPQDDK 663
                  P++   S  S+V P  D+   +    ++     +Y++ IP+DE L K+VPQ++K
Sbjct: 421  ------PSIAPSSETSSVQPTQDTICALPAVNTNTLAVVDYYAGIPYDESLGKHVPQNEK 474

Query: 662  DETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLRQXXXXXXXXXXXK 483
            DETIL+L+   + L+KEL+GWTDWANEKVMQAAR+LGKD+GELKMLRQ           K
Sbjct: 475  DETILLLISRMQTLQKELQGWTDWANEKVMQAARRLGKDKGELKMLRQEKEEAEKSQKEK 534

Query: 482  QTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEMEAATFQARASADKL 303
            Q ++EN  KRL EME+AL+N  GQI++AN ++RRLE EN++LKKEMEAA   A ASA   
Sbjct: 535  QMVEENTTKRLSEMEYALSNASGQIKMANSSLRRLEVENAVLKKEMEAAKLAAVASATNF 594

Query: 302  QDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKDRHNQFXXXXXXXX 123
              AV +E+E LKK Q+WE EK  + +  + LK++ A LE  LE+AK R NQF        
Sbjct: 595  HQAVAREKEILKKCQAWEIEKGSLQDDFSTLKQKAAHLEQELERAKKRQNQFKALSEQEE 654

Query: 122  XXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
                   ++ DS++ +RE+     K+E D+++++A++N Q
Sbjct: 655  RGKQRVLQQADSVKAEREQWGVESKMEEDNMREMAESNMQ 694


>ref|XP_016482981.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Nicotiana tabacum]
          Length = 837

 Score =  495 bits (1274), Expect = e-160
 Identities = 302/700 (43%), Positives = 406/700 (58%), Gaps = 20/700 (2%)
 Frame = -2

Query: 2042 SGSNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRN 1866
            SG  AD  +  +SA    EKG RNKRKFLS+ PLDIPVD   LSLTEFPRYE+LEEK ++
Sbjct: 37   SGECADEKSLAISA---QEKGSRNKRKFLSESPLDIPVDAPALSLTEFPRYELLEEKHQS 93

Query: 1865 VLDELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVES 1686
              + +GS+  R  +                 D I CQL ELL + L ATF +A+K+IVE 
Sbjct: 94   TPNGVGSLEGRC-HKSNEKQGVETLQHADWDDTIACQLMELLFHNLSATFQSAIKRIVEC 152

Query: 1685 GYNEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEK--DVNTPKHHSVFEGLQSLVD 1512
            GY+EE+AEW +L S ++HG KDA+S +VDGALALL +EK  D +TP    +FE L SLV+
Sbjct: 153  GYSEEIAEWVILRSGLYHGSKDAVSTIVDGALALLSREKELDSSTPL---IFEDLNSLVE 209

Query: 1511 YTLLEMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPA-- 1338
            YT+LEMI V+REVKP  T+ EAMWCLL+ DLNL++AC  EG      C  E+  ES +  
Sbjct: 210  YTMLEMICVLREVKPAFTVAEAMWCLLICDLNLLHACAIEGDLLGELCGLESPRESSSGS 269

Query: 1337 -LXXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPN------------ 1197
                             NKL + K   P AQ    E P A    QL              
Sbjct: 270  KFAQTKEASEVIQSNMDNKLQSSKPSMPIAQSLHSEVPCADVIAQLQESIYSHLHEVGIT 329

Query: 1196 -NKPVSGESAKESSFSLQESKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKNYKGRL 1020
             N+  SG   +E+  SL ++    D+               +D+LRQK+F FEK+YKGR+
Sbjct: 330  GNESSSG-LIRENILSLSKAAILEDKSGTAKRGSSK-----KDILRQKSFHFEKSYKGRM 383

Query: 1019 SKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXXXXXXVK 840
             KG+FK+K+  WGSMVLDK           VMK  YSK+T   +T               
Sbjct: 384  GKGSFKAKLTTWGSMVLDKTLNSESGSSGFVMKSTYSKVTASLKTNGPLAEGSSHSSSTS 443

Query: 839  DPVFALPAVNSKSPASTV-PDSDSCPNVEINVSDPPKPTNYFSSIPFDEILQKYVPQDDK 663
                  P++   S  S+V P  D+   +    ++     +Y++ IP+DE L K+VPQ++K
Sbjct: 444  ------PSIAPSSETSSVQPTQDTICALPAVNTNTLAVVDYYAGIPYDESLGKHVPQNEK 497

Query: 662  DETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLRQXXXXXXXXXXXK 483
            DETIL+L+   + L+KEL+GWTDWANEKVMQAAR+LGKD+GELKMLRQ           K
Sbjct: 498  DETILLLISRMQTLQKELQGWTDWANEKVMQAARRLGKDKGELKMLRQEKEEAEKSQKEK 557

Query: 482  QTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEMEAATFQARASADKL 303
            Q ++EN  KRL EME+AL+N  GQI++AN ++RRLE EN++LKKEMEAA   A ASA   
Sbjct: 558  QMVEENTTKRLSEMEYALSNASGQIKMANSSLRRLEVENAVLKKEMEAAKLAAVASATNF 617

Query: 302  QDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKDRHNQFXXXXXXXX 123
              AV +E+E LKK Q+WE EK  + +  + LK++ A LE  LE+AK R NQF        
Sbjct: 618  HQAVAREKEILKKCQAWEIEKGSLQDDFSTLKQKAAHLEQELERAKKRQNQFKALSEQEE 677

Query: 122  XXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
                   ++ DS++ +RE+     K+E D+++++A++N Q
Sbjct: 678  RGKQRVLQQADSVKAEREQWGVESKMEEDNMREMAESNMQ 717


>ref|XP_019234828.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            attenuata]
 gb|OIT06952.1| putative e3 ubiquitin-protein ligase rf298 [Nicotiana attenuata]
          Length = 813

 Score =  492 bits (1267), Expect = e-159
 Identities = 301/699 (43%), Positives = 407/699 (58%), Gaps = 19/699 (2%)
 Frame = -2

Query: 2042 SGSNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRN 1866
            SG  AD  +  +SA    EKG RNKRKFLS+ PLDIPVD   LSLTEFPRYE+LEEK ++
Sbjct: 14   SGEFADEKSLAISA---QEKGSRNKRKFLSESPLDIPVDAPALSLTEFPRYELLEEKHQS 70

Query: 1865 VLDELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVES 1686
              + +GS+  R   +                D I CQL ELL + L ATF +A+K+IVE 
Sbjct: 71   TPNGVGSLEGRCHQS-NEKQGVETLQHADWDDTIACQLMELLFHNLSATFQSAIKRIVEC 129

Query: 1685 GYNEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEK--DVNTPKHHSVFEGLQSLVD 1512
            GY+EE+AEW +L S ++HG KDA+S +VDGALALL +EK  D +TP    +FE L SLV+
Sbjct: 130  GYSEEIAEWVILRSGLYHGSKDAVSTIVDGALALLSREKELDSSTPL---IFEDLNSLVE 186

Query: 1511 YTLLEMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPALX 1332
            YT+LEMI V+REVKP  T+ EAMWCLL+ DLNL++AC  EG      C  E+  ES +  
Sbjct: 187  YTMLEMICVLREVKPAFTVAEAMWCLLICDLNLLHACAIEGDLLGELCSLESPRESSSGS 246

Query: 1331 XXXXXXXXXXXXXSN--KLDNLKQRDPRAQMFRPETPVAGPAQQLPN------------- 1197
                         SN  KL + K   P AQ    E P A    QL               
Sbjct: 247  KLAQTKEASEVIQSNVDKLQSSKPSMPIAQSLHSEVPCADVIAQLQESIYSHLHEVGITG 306

Query: 1196 NKPVSGESAKESSFSLQESKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKNYKGRLS 1017
            N+  SG   +E+  SL ++    D+               +D+LRQK+F FEK+YKGR+ 
Sbjct: 307  NESSSG-LIRENILSLSKAAILEDKSGAAKRGSSK-----KDILRQKSFHFEKSYKGRMG 360

Query: 1016 KGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXXXXXXVKD 837
            KG+FK+K+  WGSMVLDK           VMK  YSK+TT  +T                
Sbjct: 361  KGSFKAKLTTWGSMVLDKTLNSESGSSGFVMKSTYSKVTTSLKTNGPLAEGSSHSSSTS- 419

Query: 836  PVFALPAVNSKSPASTV-PDSDSCPNVEINVSDPPKPTNYFSSIPFDEILQKYVPQDDKD 660
                 P++   S  S+V P  D+   +    ++ P   +Y++ IP+DE L K+VPQ++KD
Sbjct: 420  -----PSIAPSSEISSVQPTQDTVCALPAVNANTPAVVDYYAGIPYDESLGKHVPQNEKD 474

Query: 659  ETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLRQXXXXXXXXXXXKQ 480
            ETIL+L+   + L+KEL+GWTDWANEKVMQAAR+LGKD+ ELKMLRQ           +Q
Sbjct: 475  ETILLLISRMQTLQKELQGWTDWANEKVMQAARRLGKDKEELKMLRQEKEEAEKSQKEQQ 534

Query: 479  TLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEMEAATFQARASADKLQ 300
             ++EN  KRL EME+AL+N  GQIE+AN ++RRLE ENS+LKKEMEAA   A  SA    
Sbjct: 535  MVEENTTKRLSEMEYALSNASGQIEMANSSLRRLEVENSVLKKEMEAAKLAAVESATNFH 594

Query: 299  DAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKDRHNQFXXXXXXXXX 120
             AV +EQE LKK ++W+ EK  + + ++ LK++ A LE  LE+AK   +QF         
Sbjct: 595  QAVAREQEILKKCKAWKIEKGSLQDDLSTLKQKAAHLEQELERAKRCQSQFKALSEQEER 654

Query: 119  XXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
                  ++ DS++ +RE+     K+E D+++++A++N Q
Sbjct: 655  EKQRVLQQADSVKAEREQRGVESKMEEDNMREMAESNMQ 693


>ref|XP_006355787.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Solanum tuberosum]
 ref|XP_006355788.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Solanum tuberosum]
          Length = 845

 Score =  488 bits (1255), Expect = e-157
 Identities = 295/722 (40%), Positives = 403/722 (55%), Gaps = 44/722 (6%)
 Frame = -2

Query: 2036 SNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRNVL 1860
            +++D +NE    +   EKG RNKRKFLS+ PLD+P+D   LSLTEFPRYE+LEEK  +  
Sbjct: 7    NSSDGANEKSLVISAQEKGSRNKRKFLSEFPLDVPIDTPVLSLTEFPRYELLEEKLLSTP 66

Query: 1859 DELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVESGY 1680
            +E+GS+  R   +                D I CQL ELL + L ATF +A+K+IVE GY
Sbjct: 67   NEVGSLEGRCHQS-NEKQGVETLQQADWDDTIACQLMELLFHNLSATFQSAIKRIVECGY 125

Query: 1679 NEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLVDYTLL 1500
            +EE+AE  +L S ++HG KDA+S ++DGALALL +EK+++T     +FE L SLV+YT+L
Sbjct: 126  SEEIAERVLLRSGLYHGSKDAVSTIMDGALALLSREKELDT-STSLIFEDLNSLVEYTML 184

Query: 1499 EMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEA--SGESPALXXX 1326
            EMI V+REVKP  T+ EAMWCLL+ DLNL++AC  E      SC  E+     S +    
Sbjct: 185  EMICVLREVKPDFTVAEAMWCLLICDLNLLHACSIERDLQVDSCSSESPRKSSSGSKLAQ 244

Query: 1325 XXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQ-------------------- 1206
                        +KL   K   P A+  + ETP   P  Q                    
Sbjct: 245  PKEAFVVSQLGLDKLQLSKPSMPIAKSLQSETPCDDPVTQFAQLSNSIYSHLHGVEITAN 304

Query: 1205 -----LPNNKPVSGESAKESSFSLQESKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFE 1041
                 LP  K  S   + ES  S+  +K A  E              K+D+LRQK F FE
Sbjct: 305  RSSARLPVAKSKSAGVSGESVLSI--TKAAILEEKCGTGRRGSSLNSKKDMLRQKTFHFE 362

Query: 1040 KNYKGRLSKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAET-------- 885
            K+YKGR+ KG+FK+K+  W SMVLDK          + MK   SK+TT  ++        
Sbjct: 363  KSYKGRMGKGSFKAKLTTWSSMVLDKTLNSESRSSGLGMKSTNSKVTTTVKSNCPLAVGS 422

Query: 884  --------XXXXXXXXXXXXXVKDPVFALPAVNSKSPASTVPDSDSCPNVEINVSDPPKP 729
                                  +D V ALPAVN+   AS  PD  S  N   +    PK 
Sbjct: 423  SHSSSTSPSIAPSSETATVPATQDTVCALPAVNTNIAASLTPDPKSSSNTPGSTPALPKV 482

Query: 728  TNYFSSIPFDEILQKYVPQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGK 549
             +Y++ IP+DE L+K+VPQ++KD+ +L+ +   + L+KEL+GWTDWANEKVMQAAR+LGK
Sbjct: 483  LDYYAGIPYDESLEKHVPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVMQAARRLGK 542

Query: 548  DQGELKMLRQXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEE 369
            DQGELKML+Q           KQ ++ENN+KRL EME+AL+N  GQ ++A+  + RL+EE
Sbjct: 543  DQGELKMLKQEKEEAEKLQKEKQIVEENNMKRLSEMEYALSNASGQSKMADSTLHRLKEE 602

Query: 368  NSLLKKEMEAATFQARASADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAEL 189
            N  LKKEM+AAT  A  SA     AV +EQE LKK Q+WE +K  +++  + LKR+    
Sbjct: 603  NVSLKKEMDAATLAALESATNFHQAVAREQEILKKCQAWEMKKGSLLDNFSTLKREAVHF 662

Query: 188  ENRLEKAKDRHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTN 9
            E  LE+AK R NQ                ++ DSL+ +RE+   + K+E D+I++ A+ N
Sbjct: 663  EQELERAKTRQNQLKVLSEQEEREKRKVLQQADSLKAEREKRGVQSKMEEDNIREKAERN 722

Query: 8    KQ 3
             Q
Sbjct: 723  MQ 724


>ref|XP_015078209.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Solanum pennellii]
          Length = 842

 Score =  481 bits (1237), Expect = e-154
 Identities = 297/726 (40%), Positives = 396/726 (54%), Gaps = 48/726 (6%)
 Frame = -2

Query: 2036 SNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRNVL 1860
            +++D +NE    +   EKG RNKRKFLS+ PLD+P+D   LSLTEFPRYE+LEEK  +  
Sbjct: 7    NSSDGANEKSLVISAQEKGSRNKRKFLSEFPLDVPIDTPVLSLTEFPRYELLEEKLLSTP 66

Query: 1859 DELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVESGY 1680
            +E  S+  R   +                D I CQL ELL + L ATF +A+K+IVE GY
Sbjct: 67   NEASSLEGRCHQS-NEKQGVETLQQADWDDTIACQLMELLFHNLSATFQSAIKRIVECGY 125

Query: 1679 NEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLVDYTLL 1500
            +EE AE  +L S ++HG KDA+S +VDGALALL +EK+++T   + +FE L SLV+YT+L
Sbjct: 126  SEETAERVLLRSGLYHGSKDAVSTIVDGALALLSREKELDT-STYLIFEDLNSLVEYTML 184

Query: 1499 EMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSC-----EQEASGESPAL 1335
            EMI V+REVKP  T+ EAMWCLL+ DLNL++AC  E   +  SC      + +SG  PA 
Sbjct: 185  EMICVLREVKPDFTVAEAMWCLLICDLNLLHACSIERDLSVDSCSLGSPRKSSSGSKPA- 243

Query: 1334 XXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQL---------------- 1203
                           +KL   K   P A+  + E P   P   L                
Sbjct: 244  --QPKEAFVVSQLGLDKLQLSKPSMPIAKGLQSEIPCDDPVALLSNSIYSHLHGVDITAN 301

Query: 1202 ----------PNNKPVSGESAKESSFSLQESKGATDEXXXXXXXXXXXXXXKRDLLRQKA 1053
                       N+  VSGES       L  +K A  E              K+D+LRQK 
Sbjct: 302  RSSARFPAAKSNSAGVSGESV------LSITKAAILEEKSGTGRRGSSMISKKDMLRQKT 355

Query: 1052 FQFEKNYKGRLSKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTT------PA 891
            F FEK+YKGR+ KG+FK+K+  W SMVLDK          + MK   SK+TT      P 
Sbjct: 356  FHFEKSYKGRMGKGSFKAKLTTWSSMVLDKTLNSESCSSGLAMKSTNSKVTTTVKCNVPL 415

Query: 890  ET----------XXXXXXXXXXXXXVKDPVFALPAVNSKSPASTVPDSDSCPNVEINVSD 741
            E                         +D V ALPAVN+    S  PD  S  N   +   
Sbjct: 416  EVASSHSLSTSPSIAPSSETASVPATQDTVCALPAVNTNISVSLTPDPKSSSNTPGSTPA 475

Query: 740  PPKPTNYFSSIPFDEILQKYVPQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAAR 561
            PPK  +Y++ IP+DE L KYVPQ++KD+ +L+ +   + L+KEL+GWTDWANEKVMQAAR
Sbjct: 476  PPKVLDYYAGIPYDESLGKYVPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVMQAAR 535

Query: 560  KLGKDQGELKMLRQXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRR 381
            +LGKDQ ELKML+Q           KQ ++ENN+KRL EME+AL+N  GQ ++A+  + R
Sbjct: 536  RLGKDQVELKMLKQEKEEAEKLQKEKQMVEENNMKRLSEMEYALSNASGQSKMADSTLHR 595

Query: 380  LEEENSLLKKEMEAATFQARASADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQ 201
            L+EEN  LKKEM+AAT  A  SA     AV +EQE LK  Q+WE EK  +    + LKR+
Sbjct: 596  LKEENVSLKKEMDAATLAALESATNFHQAVAREQEMLKNCQAWEMEKGSLQHNFSTLKRE 655

Query: 200  MAELENRLEKAKDRHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQI 21
                E  LE+AK R NQ                ++ DSL+ +RE+   + K+E D+I++ 
Sbjct: 656  AVHFEQELERAKTRQNQLKVLLEQEEREKRRVLQQADSLKAEREKRGVQSKMEEDNIRET 715

Query: 20   AQTNKQ 3
             + N Q
Sbjct: 716  TERNMQ 721


>ref|XP_010321841.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Solanum
            lycopersicum]
          Length = 842

 Score =  478 bits (1230), Expect = e-153
 Identities = 296/726 (40%), Positives = 393/726 (54%), Gaps = 48/726 (6%)
 Frame = -2

Query: 2036 SNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRNVL 1860
            +++D +NE    +   EKG RNKRKFLS+ PLD+P+D   LSLTEFPRYE+LEEK  +  
Sbjct: 7    NSSDGANEKSLVISAQEKGSRNKRKFLSEFPLDVPIDTPVLSLTEFPRYELLEEKLLSTP 66

Query: 1859 DELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVESGY 1680
            +E  S+  R   +                D I CQL ELL + L  TF +A+K+IVE GY
Sbjct: 67   NEASSLEGRCHQS-NEKQGVETLQQADWDDTIACQLMELLFHNLSGTFQSAIKRIVECGY 125

Query: 1679 NEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLVDYTLL 1500
            NEE AEW +L S ++HG KDA+S +VDGALALL +EK+++T   + +FE L SLV+YT+L
Sbjct: 126  NEETAEWVLLRSGLYHGSKDAVSTIVDGALALLSREKELDT-STYLIFEDLNSLVEYTML 184

Query: 1499 EMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQE-----ASGESPAL 1335
            EMI V+REVKP  T+ EAMWCLL+ DLNL++AC  E   +  SC  E     +SG  PA 
Sbjct: 185  EMICVLREVKPDFTVAEAMWCLLICDLNLLHACSIERDLSVDSCSLESPRKSSSGSKPA- 243

Query: 1334 XXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQL---------------- 1203
                           +KL   K   P A+  + E P   P   L                
Sbjct: 244  --QPKDAFVVSQLGLDKLQLSKPSMPIAKGLQSEIPCDDPVALLSNSIYSHLHGVDITAN 301

Query: 1202 ----------PNNKPVSGESAKESSFSLQESKGATDEXXXXXXXXXXXXXXKRDLLRQKA 1053
                       N+  VSGES       L  +K A  E              K+D+LRQK 
Sbjct: 302  RSSARFPAAKSNSAGVSGESV------LSITKAAILEEKNGTGRRGSSMISKKDMLRQKT 355

Query: 1052 FQFEKNYKGRLSKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTT------PA 891
            F FEK+YKGR+ KG+FK+K+  W SMVLDK          + MK   SK+TT      P 
Sbjct: 356  FHFEKSYKGRMGKGSFKAKLTTWSSMVLDKTLNSESCSSGLAMKSTNSKVTTTVKCNGPL 415

Query: 890  ET----------XXXXXXXXXXXXXVKDPVFALPAVNSKSPASTVPDSDSCPNVEINVSD 741
            E                         +D V ALPAVN+       PD  S  N   +   
Sbjct: 416  EVGSSHSSSTYPSIAPSSETASVPATQDTVCALPAVNTNISVPLTPDPKSSSNTPGSTPA 475

Query: 740  PPKPTNYFSSIPFDEILQKYVPQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAAR 561
            PPK  +Y++ IP+DE L KYVPQ++KD+ +L+ +   + L+KEL+GWTDWANEKVMQAAR
Sbjct: 476  PPKVLDYYAGIPYDESLGKYVPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVMQAAR 535

Query: 560  KLGKDQGELKMLRQXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRR 381
            +LGKDQGELKML+Q           KQ ++EN +KRL EME AL+N  GQ ++A+  + R
Sbjct: 536  RLGKDQGELKMLKQEKEEAEKLQKEKQMVEENTMKRLSEMECALSNASGQSKMADSTLHR 595

Query: 380  LEEENSLLKKEMEAATFQARASADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQ 201
            L+EEN  LKKEM+AAT  A  SA  +  AV +EQE LK  Q+ E E   +    + LKR+
Sbjct: 596  LKEENVSLKKEMDAATLAALESATNVHQAVAREQEMLKNCQALEMENGSLQHNFSTLKRE 655

Query: 200  MAELENRLEKAKDRHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQI 21
                E  LE+AK R NQ                ++ DSL+ +RE+   + K+E D+I++ 
Sbjct: 656  AVHFEQELERAKTRQNQLKVLLEQEEREKRRVLQQADSLKAEREKRGVQSKMEEDNIRET 715

Query: 20   AQTNKQ 3
             + N Q
Sbjct: 716  TERNMQ 721


>ref|XP_015078210.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Solanum pennellii]
          Length = 837

 Score =  476 bits (1226), Expect = e-153
 Identities = 297/726 (40%), Positives = 396/726 (54%), Gaps = 48/726 (6%)
 Frame = -2

Query: 2036 SNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRNVL 1860
            +++D +NE    +   EKG RNKRKFLS+ PLD+P+D   LSLTEFPRYE+LEEK  +  
Sbjct: 7    NSSDGANEKSLVISAQEKGSRNKRKFLSEFPLDVPIDTPVLSLTEFPRYELLEEKLLSTP 66

Query: 1859 DELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVESGY 1680
            +E  S+  R   +                D I CQL ELL + L ATF +A+K+IVE GY
Sbjct: 67   NEASSLEGRCHQS-NEKQGVETLQQADWDDTIACQLMELLFHNLSATFQSAIKRIVECGY 125

Query: 1679 NEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLVDYTLL 1500
            +EE AE  +L S ++HG KDA+S +VDGALALL +EK+++T   + +FE L SLV+YT+L
Sbjct: 126  SEETAERVLLRSGLYHGSKDAVSTIVDGALALLSREKELDT-STYLIFEDLNSLVEYTML 184

Query: 1499 EMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSC-----EQEASGESPAL 1335
            EMI V+REVKP  T+ EAMWCLL+ DLNL++AC  E   +  SC      + +SG  PA 
Sbjct: 185  EMICVLREVKPDFTVAEAMWCLLICDLNLLHACSIERDLSVDSCSLGSPRKSSSGSKPA- 243

Query: 1334 XXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQL---------------- 1203
                           +KL   K   P A+  + E P   P   L                
Sbjct: 244  --QPKEAFVVSQLGLDKLQLSKPSMPIAKGLQSEIPCDDPVALLSNSIYSHLHGVDITAN 301

Query: 1202 ----------PNNKPVSGESAKESSFSLQESKGATDEXXXXXXXXXXXXXXKRDLLRQKA 1053
                       N+  VSGES       L  +K A  E              K+D+LRQK 
Sbjct: 302  RSSARFPAAKSNSAGVSGESV------LSITKAAILEEKSGTGRRGSSMISKKDMLRQKT 355

Query: 1052 FQFEKNYKGRLSKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTT------PA 891
            F FEK+YKGR+ KG+FK+K+  W SMVLDK          + MK   SK+TT      P 
Sbjct: 356  FHFEKSYKGRMGKGSFKAKLTTWSSMVLDKTLNSESCSSGLAMKSTNSKVTTTVKCNVPL 415

Query: 890  ET----------XXXXXXXXXXXXXVKDPVFALPAVNSKSPASTVPDSDSCPNVEINVSD 741
            E                         +D V ALPAVN+    S  PD  S  N   +   
Sbjct: 416  EVASSHSLSTSPSIAPSSETASVPATQDTVCALPAVNTNISVSLTPDPKSSSNTPGSTPA 475

Query: 740  PPKPTNYFSSIPFDEILQKYVPQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAAR 561
            PPK  +Y++ IP+DE L KYVPQ++KD+ +L+ +   + L+KEL+GWTDWANEKVMQAAR
Sbjct: 476  PPKVLDYYAGIPYDESLGKYVPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVMQAAR 535

Query: 560  KLGKDQGELKMLRQXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRR 381
            +LGKDQ ELKML+Q           KQ ++ENN+KRL EME+AL+N  GQ ++A+  + R
Sbjct: 536  RLGKDQVELKMLKQEKEEAEKLQKEKQMVEENNMKRLSEMEYALSNASGQSKMADSTLHR 595

Query: 380  LEEENSLLKKEMEAATFQARASADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQ 201
            L+EEN  LKKEM+AAT  A  SA     AV +EQE LK  Q+WE EK  +    + LKR+
Sbjct: 596  LKEENVSLKKEMDAATLAALESATNFHQAVAREQEMLKNCQAWEMEKGSLQHNFSTLKRE 655

Query: 200  MAELENRLEKAKDRHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQI 21
                E  LE+AK R NQ                ++ DSL+ +RE+   + K+E D+I++ 
Sbjct: 656  AVHFEQELERAKTRQNQL-----KEEREKRRVLQQADSLKAEREKRGVQSKMEEDNIRET 710

Query: 20   AQTNKQ 3
             + N Q
Sbjct: 711  TERNMQ 716


>ref|XP_019228345.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            attenuata]
 ref|XP_019228352.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            attenuata]
 gb|OIT06223.1| putative e3 ubiquitin-protein ligase rf298 [Nicotiana attenuata]
          Length = 814

 Score =  471 bits (1212), Expect = e-151
 Identities = 290/713 (40%), Positives = 393/713 (55%), Gaps = 35/713 (4%)
 Frame = -2

Query: 2036 SNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRNVL 1860
            ++ D +NE    +   EKG RNKRKFLS+ P   P D   LSLTEFPRYE+LE+K ++ L
Sbjct: 5    NSTDDANEKSLVLSDQEKGSRNKRKFLSEFP---PSDSPVLSLTEFPRYELLEKKLQSTL 61

Query: 1859 DELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVESGY 1680
            ++L S+    +  +                PITCQLEELLS  L ATF +A+K+IVE GY
Sbjct: 62   NQLKSLDGGCQQGVERSADADWEE------PITCQLEELLSQNLSATFRSAVKRIVECGY 115

Query: 1679 NEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLVDYTLL 1500
            +EE+AE  +L S ++HG KD +SN+VDGALALL +EKD +T +H + F  L  LV+YT+L
Sbjct: 116  SEEIAERVILRSGLYHGTKDTVSNIVDGALALLSREKDFDTSRHLA-FSDLHGLVEYTML 174

Query: 1499 EMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPALXXXXX 1320
            EMI V+REVKP  T+ EAMWCLL+ DLNLV+AC  EG      C QE+ G S        
Sbjct: 175  EMICVLREVKPAFTVAEAMWCLLIWDLNLVHACTVEGDILDALCRQESPGNSSKTEASET 234

Query: 1319 XXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPNNKPVSGESA---KESSFSL 1149
                      +  D L+   P   + + +  VA  A Q P +K      A   K SS +L
Sbjct: 235  TL--------SNPDKLQLSKPSIPIAQSKVAVASAAPQEPTSKNSHVRQAAIGKGSSVAL 286

Query: 1148 QE-----------------SKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKNYKGRL 1020
             E                 SK A  E              K+DLLRQK FQFEKNYKGR+
Sbjct: 287  PEAEAKSLPTSSRGHKQNPSKAAVLEDKSGAGRKGSSLNSKKDLLRQKTFQFEKNYKGRM 346

Query: 1019 SKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXXXXXXV- 843
             KG+ K+K++AWG+MVLDK             K + SK+ T  +                
Sbjct: 347  GKGSIKAKLSAWGNMVLDKTLNPPSGSSGAATKSSSSKVNTSVKNNSPIAEGSSDSLCPC 406

Query: 842  ------------KDPVFALPAVNSKSPASTVPDSDSCPNVEI-NVSDPPKPTNYFSSIPF 702
                        +D +   PAVN K P+S   D  S       + +   +  +Y++ IP+
Sbjct: 407  SSTAPATDDSGVQDTIIPSPAVNKKDPSSLALDPKSSVKAPGGSTTSSSEVPDYYAGIPY 466

Query: 701  DEILQKYVPQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLR 522
            DE L KYVP++++D TIL+L PH + L+KEL+ WTDWANEKVMQAAR+LGKDQ ELKMLR
Sbjct: 467  DESLGKYVPRNERDATILLLTPHMKTLQKELQRWTDWANEKVMQAARRLGKDQAELKMLR 526

Query: 521  QXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEME 342
            Q            Q L+E   KRL EMEHAL+N  GQIELA   + RLE EN++LKKEM 
Sbjct: 527  QEKEDAERSQKETQMLEETTTKRLSEMEHALSNASGQIELAGSTLHRLEGENAVLKKEMN 586

Query: 341  AATFQARASADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKD 162
            AA   +  SA  +  A+  EQET+KK Q+ E EK  + E+++ LK + ++L+ R EK   
Sbjct: 587  AAKLFSAVSAINMHKALATEQETVKKYQAGEVEKRSLQEELSMLKHETSDLQQRQEKVNK 646

Query: 161  RHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
            R +QF               ++ DSL+ +RE+   + K   D++++ A+++ Q
Sbjct: 647  RLDQFEVLWKQEERQKQRFVQQADSLKAEREQLGVQGKAAEDNLREKAESSMQ 699


>ref|XP_009614905.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            tomentosiformis]
 ref|XP_009614906.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            tomentosiformis]
 ref|XP_016501150.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            tabacum]
 ref|XP_016501151.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            tabacum]
          Length = 814

 Score =  471 bits (1212), Expect = e-151
 Identities = 292/713 (40%), Positives = 395/713 (55%), Gaps = 35/713 (4%)
 Frame = -2

Query: 2036 SNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRNVL 1860
            ++ D +NE    +   EKG RNKRKFLS+ P   P D   LSLTEFPRYE+LE+K ++ L
Sbjct: 5    NSTDDANEKSLVLSDQEKGSRNKRKFLSEFP---PSDSPVLSLTEFPRYELLEKKLQSTL 61

Query: 1859 DELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVESGY 1680
            ++L S+    +  +                PITCQLEELLS  L ATF +A+K+IVE GY
Sbjct: 62   NQLKSLDGGCQQGVERSADADWEE------PITCQLEELLSQNLSATFRSAVKRIVECGY 115

Query: 1679 NEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLVDYTLL 1500
            +EE+AE  +L S ++HG KD +SN+VDGALALL +EKD +T +H + F  L  LV+YT+L
Sbjct: 116  SEEIAERVILRSGLYHGTKDTVSNIVDGALALLSREKDFDTSRHLA-FSDLHGLVEYTML 174

Query: 1499 EMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPALXXXXX 1320
            EMI V+REVKP  T+ EAMWCLL+ DLNLV+AC  EG      C QE+ G+S        
Sbjct: 175  EMICVLREVKPAFTVAEAMWCLLIWDLNLVHACTVEGDILDELCSQESLGDS-------- 226

Query: 1319 XXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPNNKPVSGESA---KESSFSL 1149
                      +  D L+   P   + + +  VA  A Q P +K      A   K SS  L
Sbjct: 227  LKTEASETTQSNPDKLQLSKPSIAIAQSKVAVASAAPQEPTSKNSHVRQAAIGKGSSVPL 286

Query: 1148 QE-----------------SKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKNYKGRL 1020
             E                 SK A  E              K+DLLRQK FQFEKNYKGR+
Sbjct: 287  PEAEAKSLPTSSRGHKQNPSKAAVLEDKSGAGRKGSSLNSKKDLLRQKTFQFEKNYKGRM 346

Query: 1019 SKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXXXXXXV- 843
             KG+ K+K++AWG+MVLDK             K + SK+ T  +                
Sbjct: 347  GKGSIKAKLSAWGNMVLDKTLNPPSGSSGAATKSSSSKVNTSVKNSSPIAEGSSDSLCPC 406

Query: 842  ------------KDPVFALPAVNSKSPASTVPDSDSCPNVEI-NVSDPPKPTNYFSSIPF 702
                        +D V   PAVN K P+S   D  S       + +   +  +Y++ IP+
Sbjct: 407  SSTAPATDDSGVQDTVITSPAVNKKDPSSLALDPKSSIKAPGGSTTSSSEVPDYYAGIPY 466

Query: 701  DEILQKYVPQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLR 522
            DE L KYVPQ+++D  IL+L PH + L+KEL+ WTDWANEKVMQAAR+LGKDQ ELKMLR
Sbjct: 467  DESLGKYVPQNERDAAILLLTPHMKTLQKELQRWTDWANEKVMQAARRLGKDQAELKMLR 526

Query: 521  QXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEME 342
            Q           KQ L+E+  KRL EMEHAL+N  GQIELA   + RLE EN++LKKEM+
Sbjct: 527  QEKEDAERSQKEKQMLEESTTKRLSEMEHALSNASGQIELAGSTLHRLEGENAVLKKEMD 586

Query: 341  AATFQARASADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKD 162
            AA   +  SA  +  A+  EQET+KK Q+ E EK  + E+++ LK + ++L+ R EK   
Sbjct: 587  AAKLFSAVSAINMHKALATEQETVKKYQAGEVEKRSLQEELSMLKHETSDLQQRQEKVNK 646

Query: 161  RHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
            R +QF               ++ DSL+ +RE+   + K   D++++ A+++ Q
Sbjct: 647  RLDQFEVLWKQEERQKQRFVQQADSLKAEREQLGVQGKAAEDNLREKAESSMQ 699


>ref|XP_009798672.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            sylvestris]
 ref|XP_009798673.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            sylvestris]
 ref|XP_009798674.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            sylvestris]
          Length = 814

 Score =  461 bits (1186), Expect = e-147
 Identities = 288/713 (40%), Positives = 389/713 (54%), Gaps = 35/713 (4%)
 Frame = -2

Query: 2036 SNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRNVL 1860
            ++ D SNE        EKG RNKRKFLS+ P   P D   LSLTEFPRYE+LE+K  + L
Sbjct: 5    NSTDDSNEKSLVHSDQEKGSRNKRKFLSEFP---PSDSPVLSLTEFPRYELLEKKLHSTL 61

Query: 1859 DELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVESGY 1680
            ++L S+    +  +                PITCQLEELLS  L ATF +A+K+IVE GY
Sbjct: 62   NQLKSLDGGCQQGVERSADGDWEE------PITCQLEELLSQNLSATFRSAVKRIVECGY 115

Query: 1679 NEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLVDYTLL 1500
            +EE+AE  +L S ++HG KD +SN+VDGALALL +EKD +T + H  F  L  LV+YT+L
Sbjct: 116  SEEIAERVILRSGLYHGTKDTVSNIVDGALALLSREKDFDTSR-HLAFSDLHGLVEYTML 174

Query: 1499 EMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPALXXXXX 1320
            EM+ V+REVKP  T+ EAMWCLL+ DLNLV+AC  EG      C QE+ G S        
Sbjct: 175  EMVCVLREVKPAFTVAEAMWCLLIWDLNLVHACTVEGDILDELCSQESPGNS-------- 226

Query: 1319 XXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPNNKPVSGESA---KESSFSL 1149
                      +  D L+   P   + + +  VA  A Q P +K      A   K SS  L
Sbjct: 227  SKTEASETTQSNPDKLQLSKPSIPIAQSKVAVASAAPQEPTSKNSHVRQAAIGKGSSVPL 286

Query: 1148 QE-----------------SKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKNYKGRL 1020
             E                 SK A  E              K+DLLRQK FQFEKNYKGR+
Sbjct: 287  PEAEAKSLPTSSRGHKQNPSKAAVLEDKLGAGRKGSSLNSKKDLLRQKTFQFEKNYKGRM 346

Query: 1019 SKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAET-------------XX 879
             KG+ K+K++AWG+MV+DK             K + SK+ T  +                
Sbjct: 347  GKGSIKAKLSAWGNMVMDKTLNPLSGSSGAATKSSSSKVNTSVKNNSPIAEGSSDSLRPC 406

Query: 878  XXXXXXXXXXXVKDPVFALPAVNSKSPASTVPDSDSCPNVE-INVSDPPKPTNYFSSIPF 702
                       V+  V   PAVN K P+S   D  S       + +   +  +Y++ I +
Sbjct: 407  SSTAPATDDSGVQHTVIPSPAVNKKDPSSLALDPKSSVKAPGGSTTSSSEVPDYYAGIAY 466

Query: 701  DEILQKYVPQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLR 522
            DE L KYVPQ+++D  IL+L PH + L+KEL+ WTDWANEKVMQAAR+LGKDQ ELKMLR
Sbjct: 467  DESLGKYVPQNERDAAILLLTPHMKTLQKELQRWTDWANEKVMQAARRLGKDQAELKMLR 526

Query: 521  QXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEME 342
            Q            Q L+E+  KRL EMEHAL+N  GQIELA   + RLE EN++LKKEM+
Sbjct: 527  QEKEDAERSQKETQMLEESTTKRLSEMEHALSNASGQIELAGSTLHRLEGENAVLKKEMD 586

Query: 341  AATFQARASADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKD 162
            AA   +  SA  +  A+  EQET+KK Q+ E EK  + E+++ LK + ++L+ R EK   
Sbjct: 587  AAKLFSAVSAINMHKALATEQETVKKYQAGEVEKRSLQEELSMLKHETSDLQQRQEKVNK 646

Query: 161  RHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
            R +QF               ++ DSL+ +RE+   + K   +++++ A+++ Q
Sbjct: 647  RLDQFEVLWKQEERQKQRFVQQADSLKAEREQLGVQGKAAENNLREKAESSMQ 699


>ref|XP_016463481.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            tabacum]
 ref|XP_016463482.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Nicotiana
            tabacum]
          Length = 814

 Score =  460 bits (1184), Expect = e-147
 Identities = 288/713 (40%), Positives = 388/713 (54%), Gaps = 35/713 (4%)
 Frame = -2

Query: 2036 SNADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRNVL 1860
            ++ D SNE        EKG RNKRKFLS+ P   P D   LSLTEFPRYE+LE+K  + L
Sbjct: 5    NSTDDSNEKSLVHSDQEKGSRNKRKFLSEFP---PSDSPVLSLTEFPRYELLEKKLHSTL 61

Query: 1859 DELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVESGY 1680
            ++L S+       +                PITCQLEELLS  L ATF +A+K+IVE GY
Sbjct: 62   NQLKSLDGGCHQGVERSADGDWEE------PITCQLEELLSQNLSATFRSAVKRIVECGY 115

Query: 1679 NEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLVDYTLL 1500
            +EE+AE  +L S ++HG KD +SN+VDGALALL +EKD +T + H  F  L  LV+YT+L
Sbjct: 116  SEEIAERVILRSGLYHGTKDTVSNIVDGALALLSREKDFDTSR-HLAFSDLHGLVEYTML 174

Query: 1499 EMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPALXXXXX 1320
            EM+ V+REVKP  T+ EAMWCLL+ DLNLV+AC  EG      C QE+ G S        
Sbjct: 175  EMVCVLREVKPAFTVAEAMWCLLIWDLNLVHACTVEGDILDELCSQESPGNS-------- 226

Query: 1319 XXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQLPNNKPVSGESA---KESSFSL 1149
                      +  D L+   P   + + +  VA  A Q P +K      A   K SS  L
Sbjct: 227  SKTEASETTQSNPDKLQLSKPSIPIAQSKVAVASAAPQEPTSKNSHVRQAAIGKGSSVPL 286

Query: 1148 QE-----------------SKGATDEXXXXXXXXXXXXXXKRDLLRQKAFQFEKNYKGRL 1020
             E                 SK A  E              K+DLLRQK FQFEKNYKGR+
Sbjct: 287  PEAEAKSLPTSSRGHKQNPSKAAVLEDKLGAGRKGSSLNSKKDLLRQKTFQFEKNYKGRM 346

Query: 1019 SKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAET-------------XX 879
             KG+ K+K++AWG+MV+DK             K + SK+ T  +                
Sbjct: 347  GKGSIKAKLSAWGNMVMDKTLNPLSGSSGAATKSSSSKVNTSVKNNSPIAEGSSDSLRPC 406

Query: 878  XXXXXXXXXXXVKDPVFALPAVNSKSPASTVPDSDSCPNVE-INVSDPPKPTNYFSSIPF 702
                       V+  V   PAVN K P+S   D  S       + +   +  +Y++ I +
Sbjct: 407  SSTAPATDDSGVQHTVIPSPAVNKKDPSSLALDPKSSVKAPGGSTTSSSEVPDYYAGIAY 466

Query: 701  DEILQKYVPQDDKDETILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLR 522
            DE L KYVPQ+++D  IL+L PH + L+KEL+ WTDWANEKVMQAAR+LGKDQ ELKMLR
Sbjct: 467  DESLGKYVPQNERDAAILLLTPHMKTLQKELQRWTDWANEKVMQAARRLGKDQAELKMLR 526

Query: 521  QXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEME 342
            Q            Q L+E+  KRL EMEHAL+N  GQIELA   + RLE EN++LKKEM+
Sbjct: 527  QEKEDAERSQKETQMLEESTTKRLSEMEHALSNASGQIELAGSTLHRLEGENAVLKKEMD 586

Query: 341  AATFQARASADKLQDAVVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKD 162
            AA   +  SA  +  A+  EQET+KK Q+ E EK  + E+++ LK + ++L+ R EK   
Sbjct: 587  AAKLFSAVSAINMHKALATEQETVKKYQAGEVEKRSLQEELSMLKHETSDLQQRQEKVNK 646

Query: 161  RHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
            R +QF               ++ DSL+ +RE+   + K   +++++ A+++ Q
Sbjct: 647  RLDQFEVLWKQEERQKQRFVQQADSLKAEREQLGVQGKAAENNLREKAESSMQ 699


>ref|XP_015168014.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Solanum tuberosum]
          Length = 819

 Score =  444 bits (1141), Expect = e-140
 Identities = 272/678 (40%), Positives = 372/678 (54%), Gaps = 43/678 (6%)
 Frame = -2

Query: 1907 EFPRYEMLEEKFRNVLDELGSMMERSEYNIXXXXXXXXXXXXXXXDPITCQLEELLSNAL 1728
            EFPRYE+LEEK  +  +E+GS+  R   +                D I CQL ELL + L
Sbjct: 25   EFPRYELLEEKLLSTPNEVGSLEGRCHQS-NEKQGVETLQQADWDDTIACQLMELLFHNL 83

Query: 1727 YATFCNAMKKIVESGYNEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKH 1548
             ATF +A+K+IVE GY+EE+AE  +L S ++HG KDA+S ++DGALALL +EK+++T   
Sbjct: 84   SATFQSAIKRIVECGYSEEIAERVLLRSGLYHGSKDAVSTIMDGALALLSREKELDT-ST 142

Query: 1547 HSVFEGLQSLVDYTLLEMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSC 1368
              +FE L SLV+YT+LEMI V+REVKP  T+ EAMWCLL+ DLNL++AC  E      SC
Sbjct: 143  SLIFEDLNSLVEYTMLEMICVLREVKPDFTVAEAMWCLLICDLNLLHACSIERDLQVDSC 202

Query: 1367 EQEA--SGESPALXXXXXXXXXXXXXXSNKLDNLKQRDPRAQMFRPETPVAGPAQQ---- 1206
              E+     S +                +KL   K   P A+  + ETP   P  Q    
Sbjct: 203  SSESPRKSSSGSKLAQPKEAFVVSQLGLDKLQLSKPSMPIAKSLQSETPCDDPVTQFAQL 262

Query: 1205 ---------------------LPNNKPVSGESAKESSFSLQESKGATDEXXXXXXXXXXX 1089
                                 LP  K  S   + ES  S+  +K A  E           
Sbjct: 263  SNSIYSHLHGVEITANRSSARLPVAKSKSAGVSGESVLSI--TKAAILEEKCGTGRRGSS 320

Query: 1088 XXXKRDLLRQKAFQFEKNYKGRLSKGAFKSKVAAWGSMVLDKXXXXXXXXXSVVMKGNYS 909
               K+D+LRQK F FEK+YKGR+ KG+FK+K+  W SMVLDK          + MK   S
Sbjct: 321  LNSKKDMLRQKTFHFEKSYKGRMGKGSFKAKLTTWSSMVLDKTLNSESRSSGLGMKSTNS 380

Query: 908  KLTTPAET----------------XXXXXXXXXXXXXVKDPVFALPAVNSKSPASTVPDS 777
            K+TT  ++                              +D V ALPAVN+   AS  PD 
Sbjct: 381  KVTTTVKSNCPLAVGSSHSSSTSPSIAPSSETATVPATQDTVCALPAVNTNIAASLTPDP 440

Query: 776  DSCPNVEINVSDPPKPTNYFSSIPFDEILQKYVPQDDKDETILMLVPHKEALEKELRGWT 597
             S  N   +    PK  +Y++ IP+DE L+K+VPQ++KD+ +L+ +   + L+KEL+GWT
Sbjct: 441  KSSSNTPGSTPALPKVLDYYAGIPYDESLEKHVPQNEKDKIVLLRIARIQTLQKELQGWT 500

Query: 596  DWANEKVMQAARKLGKDQGELKMLRQXXXXXXXXXXXKQTLQENNIKRLCEMEHALANTK 417
            DWANEKVMQAAR+LGKDQGELKML+Q           KQ ++ENN+KRL EME+AL+N  
Sbjct: 501  DWANEKVMQAARRLGKDQGELKMLKQEKEEAEKLQKEKQIVEENNMKRLSEMEYALSNAS 560

Query: 416  GQIELANCAVRRLEEENSLLKKEMEAATFQARASADKLQDAVVKEQETLKKMQSWEAEKS 237
            GQ ++A+  + RL+EEN  LKKEM+AAT  A  SA     AV +EQE LKK Q+WE +K 
Sbjct: 561  GQSKMADSTLHRLKEENVSLKKEMDAATLAALESATNFHQAVAREQEILKKCQAWEMKKG 620

Query: 236  LVMEKVTNLKRQMAELENRLEKAKDRHNQFXXXXXXXXXXXXXXXKRIDSLRGKREEDEA 57
             +++  + LKR+    E  LE+AK R NQ                ++ DSL+ +RE+   
Sbjct: 621  SLLDNFSTLKREAVHFEQELERAKTRQNQLKVLSEQEEREKRKVLQQADSLKAEREKRGV 680

Query: 56   RLKVEADSIKQIAQTNKQ 3
            + K+E D+I++ A+ N Q
Sbjct: 681  QSKMEEDNIREKAERNMQ 698


>ref|XP_016565617.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Capsicum
            annuum]
          Length = 798

 Score =  389 bits (998), Expect = e-119
 Identities = 272/697 (39%), Positives = 371/697 (53%), Gaps = 21/697 (3%)
 Frame = -2

Query: 2030 ADSSNEGLSAVLVNEKG-RNKRKFLSDLPLDIPVDVSTLSLTEFPRYEMLEEKFRN-VLD 1857
            AD +N   S V   EKG RNKRKF  + PL+ P D   LSLTEFPRYE+LEE   +  L 
Sbjct: 2    ADDANGNWSVVSDQEKGSRNKRKF--EFPLETPTDSPALSLTEFPRYELLEEVLNDDTLI 59

Query: 1856 ELGSMMERS-EYNIXXXXXXXXXXXXXXXDPITCQLEELLSNALYATFCNAMKKIVESGY 1680
            E   + +R  +YN                D I+ +L ELL   L+  F +A+K+I ESGY
Sbjct: 60   EFEPLTDRHPQYN---EEPLESLTDADWEDSISWELLELLHQDLFTIFQSAVKRISESGY 116

Query: 1679 NEEVAEWAVLNSSVFHGRKDALSNVVDGALALLKKEKDVNTPKHHSVFEGLQSLVDYTLL 1500
            +EE A   +L S ++HG KDA+SN+VDGALALL   K ++T +H  VF  L+SLVDYTLL
Sbjct: 117  SEETAVRVILRSGLYHGNKDAVSNIVDGALALLSSGKGLDTGRH-LVFADLRSLVDYTLL 175

Query: 1499 EMIHVVREVKPTLTITEAMWCLLLSDLNLVNACVPEGGATSGSCEQEASGESPALXXXXX 1320
            EMI V+R VKP LT+ EAMW LL+ DLNLV+AC          C QE+ G S +      
Sbjct: 176  EMIGVLRAVKPVLTVAEAMWWLLMLDLNLVHACTVGSDLLDELCNQESPGNSSSALELSQ 235

Query: 1319 XXXXXXXXXSNKLDNL---KQRDPRAQMFRPETPVAGPAQQLPNNKPVSGESA---KESS 1158
                         DN    K     AQ  + + PVA  A +   +K +  + A   K SS
Sbjct: 236  SKTGASGNTQLNPDNQQLSKNSTEIAQTLQSKVPVASAAPEESKSKNLHVQPAAQGKGSS 295

Query: 1157 FSLQESKGATDEXXXXXXXXXXXXXXK--RDLLRQKAFQFEKNYKGRLSKGAFKSKVAAW 984
              L E++                      +DL  ++  Q+E+N + R  K   K+ + A 
Sbjct: 296  VPLPEAEAKLKAAVLEDKSGAGRKVLNSRKDLFGRRN-QYERNCRCRTKK--IKANITAL 352

Query: 983  GSMVLDKXXXXXXXXXSVVMKGNYSKLTTPAETXXXXXXXXXXXXXVKDPVF------AL 822
            GSMVLDK             KG++SK+ T  ++                 +       +L
Sbjct: 353  GSMVLDKTVNSSASSG-ATKKGSHSKVNTLVKSNKALAKASSDSPCSSSSIAPSTDTSSL 411

Query: 821  PA----VNSKSPASTVPDSDSCPNVEINVSDPPKPTNYFSSIPFDEILQKYVPQDDKDET 654
            P     +N K P S+  +  S       +     P +Y + IP+DE L KYVPQD +DE 
Sbjct: 412  PPAEDNINKKDPDSSAMEPKSSIKAHSTIISSAVP-DYCTGIPYDESLGKYVPQDGRDED 470

Query: 653  ILMLVPHKEALEKELRGWTDWANEKVMQAARKLGKDQGELKMLRQXXXXXXXXXXXKQTL 474
            IL+LVP  + L+KEL+GW+DWANEKVMQA R+LGK+Q ELKMLRQ           KQ L
Sbjct: 471  ILVLVPRLKTLQKELQGWSDWANEKVMQATRRLGKNQAELKMLRQEKKDVEKVHKEKQIL 530

Query: 473  QENNIKRLCEMEHALANTKGQIELANCAVRRLEEENSLLKKEMEAATFQARASADKLQDA 294
            +EN +KRL EME  L NT  QIE AN  V RL EE+ +LKKEME     A+ SA  + +A
Sbjct: 531  EENTMKRLMEMEQVLVNTNYQIETANSDVSRLGEESGVLKKEMETLKLCAKISAISMDNA 590

Query: 293  VVKEQETLKKMQSWEAEKSLVMEKVTNLKRQMAELENRLEKAKDRHNQFXXXXXXXXXXX 114
            + KE ET+KK Q+ E EK L+ E+++ LK++ A+ + +  KAK R +QF           
Sbjct: 591  LAKELETVKKCQAAEVEKHLLQEQLSTLKQESADSQPQQGKAKKRLDQFEVLRKQEEKVK 650

Query: 113  XXXXKRIDSLRGKREEDEARLKVEADSIKQIAQTNKQ 3
                ++  SL+ KRE+  A+ K+E D++++ A+ N Q
Sbjct: 651  QRFLQQAASLKAKREQLVAQGKLEEDNLREPAKRNLQ 687


>gb|PIN13412.1| putative E3 ubiquitin ligase [Handroanthus impetiginosus]
          Length = 570

 Score =  367 bits (943), Expect = e-114
 Identities = 216/443 (48%), Positives = 268/443 (60%), Gaps = 26/443 (5%)
 Frame = -2

Query: 1253 AQMFRPETPVAGPAQQLPNNKPVSGESAKESSFSLQ-------------ESKGATDEXXX 1113
            A+  +PETPVA PA + PN+KP  GESA  SSF+ +              S+G   +   
Sbjct: 5    AEGSQPETPVARPAHRPPNHKPSPGESA--SSFAQETKGKLSGGGRQNLSSQGMVIDGKL 62

Query: 1112 XXXXXXXXXXXKRDLLRQKAFQFEKNYKGRLSKGAFKSKVAAWGSMVLDKXXXXXXXXXS 933
                       KRD+LRQK F  EKN+KGRLSKG FK+KVAAWGSMV+DK          
Sbjct: 63   GANRGGLSINSKRDMLRQKTFHSEKNHKGRLSKGVFKAKVAAWGSMVVDKALKSQSGSSG 122

Query: 932  VVMKGNYSKLTTPAETXXXXXXXXXXXXXV------------KDPVFALPAVNSKSPAST 789
            VVMKGN S LTT A T                          KD    LPAV+S  P S+
Sbjct: 123  VVMKGNSSNLTTAAGTNSSLMEGNHHLLSNSRSGVVAGTSSVKDSACTLPAVDSTYPGSS 182

Query: 788  VPDSDSCPNV-EINVSDPPKPTNYFSSIPFDEILQKYVPQDDKDETILMLVPHKEALEKE 612
             P+++  P   + NVSD P   NY++ IPFDE LQKYVP DDKDE IL LVPHKEAL +E
Sbjct: 183  APNTNIGPKGGQTNVSDAPHTFNYYALIPFDETLQKYVPVDDKDEAILKLVPHKEALNEE 242

Query: 611  LRGWTDWANEKVMQAARKLGKDQGELKMLRQXXXXXXXXXXXKQTLQENNIKRLCEMEHA 432
            L+GWTDWAN+K+MQAAR+LGKDQGELK LRQ           KQ L+E+ +KRL EME  
Sbjct: 243  LQGWTDWANKKIMQAARRLGKDQGELKKLRQEREELEKLKKEKQILEESTVKRLSEMECK 302

Query: 431  LANTKGQIELANCAVRRLEEENSLLKKEMEAATFQARASADKLQDAVVKEQETLKKMQSW 252
            L+ T GQIE+ N ++RRLEEENSLLK++M  A  QA  SA  LQ+A+++EQE L+K+Q  
Sbjct: 303  LSTTTGQIEVGNSSIRRLEEENSLLKEDMVVAKLQAVKSATNLQEAMLREQEALRKLQLC 362

Query: 251  EAEKSLVMEKVTNLKRQMAELENRLEKAKDRHNQFXXXXXXXXXXXXXXXKRIDSLRGKR 72
            + EK L++E++ NLK Q AEL   L KAK+RHNQF                ++DSLR K+
Sbjct: 363  DTEKGLLLEQLANLKCQKAELVGPLAKAKERHNQFKALSTHEAKENLKVAAQVDSLRRKK 422

Query: 71   EEDEARLKVEADSIKQIAQTNKQ 3
            EED A  K + D+IKQ A+ N Q
Sbjct: 423  EEDNALTKADEDNIKQTAEKNMQ 445