BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00008536
         (811 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_012843328.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [E...   345   e-113
gb|KZV33003.1| 4-coumarate--CoA ligase-like 5 [Dorcoceras hygrom...   335   e-109
gb|PIN24475.1| Acyl-CoA synthetase [Handroanthus impetiginosus]       330   e-107
ref|XP_020548878.1| LOW QUALITY PROTEIN: 4-coumarate--CoA ligase...   325   e-105
ref|XP_022841470.1| 4-coumarate--CoA ligase-like 5 [Olea europae...   315   e-101
gb|PIN04540.1| Acyl-CoA synthetase [Handroanthus impetiginosus]       310   4e-99
ref|XP_023751376.1| 4-coumarate--CoA ligase 1-like [Lactuca sati...   295   3e-93
gb|KVI05000.1| AMP-binding, conserved site-containing protein [C...   290   1e-91
ref|XP_024167799.1| 4-coumarate--CoA ligase-like 5 [Rosa chinensis]   290   3e-91
gb|OTG07932.1| putative AMP-dependent synthetase/ligase, AMP-bin...   288   4e-91
gb|PRQ56377.1| putative AMP-dependent synthetase/ligase, AMP-bin...   290   4e-91
ref|XP_015876656.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Z...   289   7e-91
ref|XP_021993469.1| 4-coumarate--CoA ligase-like 5 [Helianthus a...   288   7e-91
ref|XP_018848044.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [J...   288   1e-90
gb|KVI09677.1| AMP-binding, conserved site-containing protein [C...   286   4e-90
ref|XP_016470340.1| PREDICTED: 4-coumarate--CoA ligase-like 4 is...   284   7e-90
ref|XP_002271586.3| PREDICTED: 4-coumarate--CoA ligase-like 5 [V...   286   7e-90
ref|XP_019198798.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [I...   286   2e-89
ref|XP_009766303.1| PREDICTED: 4-coumarate--CoA ligase-like 4 [N...   284   5e-89
ref|XP_004308531.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [F...   283   9e-89

>ref|XP_012843328.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Erythranthe guttata]
 gb|EYU32443.1| hypothetical protein MIMGU_mgv1a019706mg [Erythranthe guttata]
          Length = 563

 Score =  345 bits (885), Expect = e-113
 Identities = 180/236 (76%), Positives = 200/236 (84%)
 Frame = -3

Query: 710 TEEEMESLPNPLLFDKKSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPDH 531
           T EE  S+PN   FD K+GF+ QT IYHSL+ L + QKIPS PNLDIATYVLS FP PD 
Sbjct: 5   TSEEESSIPNS--FDNKNGFNPQTGIYHSLVQLNEHQKIPSHPNLDIATYVLSHFPPPDK 62

Query: 530 AQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICLS 351
           A+ +VA IDS TG RLTYAQLH SITSLA+GLYHGLGL+KGDVVF+LSPNS LYPIICL+
Sbjct: 63  AEKQVAYIDSTTGRRLTYAQLHRSITSLASGLYHGLGLRKGDVVFILSPNSLLYPIICLA 122

Query: 350 VLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSKECDQ 171
            L IGA+ITTANPLNT+REI KQVRDSRA LAISAPEETHKL+ T+VPI+LT  +K+  Q
Sbjct: 123 ALHIGAIITTANPLNTEREIVKQVRDSRATLAISAPEETHKLLPTRVPILLT--AKKDGQ 180

Query: 170 LSVEELIEKCEPLDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
           LSVEELIE CE ++IPE KP QSD AAILYSSGTTGTSKGVVLTHSNFISVVTLLK
Sbjct: 181 LSVEELIENCEYIEIPEAKPTQSDVAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 236


>gb|KZV33003.1| 4-coumarate--CoA ligase-like 5 [Dorcoceras hygrometricum]
          Length = 592

 Score =  335 bits (860), Expect = e-109
 Identities = 172/245 (70%), Positives = 205/245 (83%)
 Frame = -3

Query: 737 EEKSTKMAITEEEMESLPNPLLFDKKSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYV 558
           EE+   MA  EE+  S+ N +  D++SG++ QT IY+SL+ L++ Q+IP+QPNL+ ATYV
Sbjct: 3   EERWMGMATIEED--SVTNTMTMDRRSGYEPQTGIYYSLVSLSEKQQIPTQPNLNTATYV 60

Query: 557 LSQFPSPDHAQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNS 378
           LSQFP+PD A+TRVALI+S+T  R+TYAQLH S+T LAAGLYHGLG++KGDVVF+LSPNS
Sbjct: 61  LSQFPTPDQAETRVALIESSTNRRVTYAQLHRSVTVLAAGLYHGLGIRKGDVVFILSPNS 120

Query: 377 SLYPIICLSVLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIIL 198
            +YP ICL+VL IGAVITTANPLNT  EIEKQV DS A +AIS+PEE  KL  T VP +L
Sbjct: 121 LMYPTICLAVLSIGAVITTANPLNTSTEIEKQVHDSGAKVAISSPEEIKKLHRTGVPTLL 180

Query: 197 TKPSKECDQLSVEELIEKCEPLDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISV 18
           T  S+E DQLSVEELIEKCEPL+IPE KP Q+DTAAILYSSGTTGTSKGVVLTHSNFISV
Sbjct: 181 TSRSRETDQLSVEELIEKCEPLEIPEEKPIQTDTAAILYSSGTTGTSKGVVLTHSNFISV 240

Query: 17  VTLLK 3
           +TLLK
Sbjct: 241 ITLLK 245


>gb|PIN24475.1| Acyl-CoA synthetase [Handroanthus impetiginosus]
          Length = 558

 Score =  330 bits (845), Expect = e-107
 Identities = 173/239 (72%), Positives = 194/239 (81%)
 Frame = -3

Query: 719 MAITEEEMESLPNPLLFDKKSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPS 540
           M I EEE +      +F K+SGFD++T IYHSL HL Q QKIP+QPNLD AT+VLSQFP 
Sbjct: 1   MTIVEEESQ------MFHKRSGFDAKTGIYHSLAHLNQHQKIPTQPNLDTATFVLSQFPP 54

Query: 539 PDHAQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPII 360
           P+HA+TRV LIDS T H LTY QL  SITSLA GLYHGLGL+KGDVV +LSPNS LYP I
Sbjct: 55  PEHAETRVVLIDSMTNHELTYVQLRRSITSLAGGLYHGLGLRKGDVVLILSPNSLLYPTI 114

Query: 359 CLSVLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSKE 180
           CL+VL IGAVITTANPLNT+ EI KQV DS A LAIS+PEE HKL +T VP +LT  SK+
Sbjct: 115 CLAVLSIGAVITTANPLNTETEIAKQVCDSGAKLAISSPEEIHKLFTTGVPTLLTCRSKD 174

Query: 179 CDQLSVEELIEKCEPLDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
            +QLSVEELIE CE L+IPE K +QSDTAAILYSSGTTGTSKGVVLTHSN ISV+TLLK
Sbjct: 175 DNQLSVEELIENCEALEIPEVKQSQSDTAAILYSSGTTGTSKGVVLTHSNIISVITLLK 233


>ref|XP_020548878.1| LOW QUALITY PROTEIN: 4-coumarate--CoA ligase-like 5 [Sesamum
           indicum]
          Length = 578

 Score =  325 bits (833), Expect = e-105
 Identities = 166/239 (69%), Positives = 194/239 (81%)
 Frame = -3

Query: 719 MAITEEEMESLPNPLLFDKKSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPS 540
           MA+   E E +   LLFDK+SGFD+Q+ IY+SL+HL++  KIP+QPNLD AT+VLSQFP 
Sbjct: 1   MAMAGSEEEPVSQSLLFDKRSGFDAQSGIYYSLVHLSEQNKIPTQPNLDTATFVLSQFPP 60

Query: 539 PDHAQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPII 360
           P+HA+  VALIDSAT  RLTYA+L  SI +LAAGLYHGLGL+KGDVV +LSPNS LY  I
Sbjct: 61  PEHAEKLVALIDSATNRRLTYAKLRRSINTLAAGLYHGLGLRKGDVVLILSPNSLLYSTI 120

Query: 359 CLSVLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSKE 180
           CL+VL IG VITTANPLNT+ EI KQV DS   LA+S+PEE HK+  T VP++LT    +
Sbjct: 121 CLAVLSIGGVITTANPLNTEMEIAKQVSDSGPKLAVSSPEEMHKVSPTGVPVLLTSRGTD 180

Query: 179 CDQLSVEELIEKCEPLDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
             QLSVEELIEKCEPL+IP  KP QSDTAAILYSSGTTGTSKGVVLTH+NFISV+TLLK
Sbjct: 181 DSQLSVEELIEKCEPLEIPGVKPTQSDTAAILYSSGTTGTSKGVVLTHANFISVITLLK 239


>ref|XP_022841470.1| 4-coumarate--CoA ligase-like 5 [Olea europaea var. sylvestris]
          Length = 562

 Score =  315 bits (806), Expect = e-101
 Identities = 167/240 (69%), Positives = 195/240 (81%), Gaps = 1/240 (0%)
 Frame = -3

Query: 719 MAITEEEMESLPNPLLFDKKSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPS 540
           MA  EE+  SL    LFDK SGF+S+T IYHSL+HL++D +IP+QPNLD ATYVLSQFP 
Sbjct: 1   MATIEEQSTSLVQ--LFDKTSGFNSETGIYHSLVHLSEDDRIPTQPNLDTATYVLSQFPP 58

Query: 539 PDHAQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPII 360
            D A++RVALIDS T  ++TY++L  S+ +LAAGLYH LG+KKGDVVFVLSPNS LYP I
Sbjct: 59  LDQAESRVALIDSTTHEQVTYSRLDRSVRALAAGLYHRLGVKKGDVVFVLSPNSLLYPTI 118

Query: 359 CLSVLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSKE 180
           CL+VL IGAV+TTANPLNT  EI KQVRDS A LAI +PEE HKLV T VP ++T  SKE
Sbjct: 119 CLAVLSIGAVLTTANPLNTVAEIAKQVRDSGAKLAIVSPEEMHKLVPTGVPTLVTSYSKE 178

Query: 179 CDQ-LSVEELIEKCEPLDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
            D  L+++EL+E CEPL IPE KP QSDTAAILYSSGTTGTSKGVVLTHSNFIS++ LLK
Sbjct: 179 FDNALTIKELVENCEPLCIPEAKPVQSDTAAILYSSGTTGTSKGVVLTHSNFISIMKLLK 238


>gb|PIN04540.1| Acyl-CoA synthetase [Handroanthus impetiginosus]
          Length = 562

 Score =  310 bits (793), Expect = 4e-99
 Identities = 169/240 (70%), Positives = 196/240 (81%), Gaps = 1/240 (0%)
 Frame = -3

Query: 719 MAITEEEMESLPNPLLFDK-KSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFP 543
           MAI ++E  SLPN  +FDK +SGF+ QT IYHSL HL +   IPS PNL+ ATYVLSQFP
Sbjct: 1   MAIPKQE--SLPNSPIFDKNRSGFNPQTGIYHSLHHLPKHHIIPSHPNLNTATYVLSQFP 58

Query: 542 SPDHAQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPI 363
            P+ A+T+VALI SAT +RLTY+QL+ SITSLAAGLYHGLG++KGDVVF+LSPNS LYP 
Sbjct: 59  PPEQAETKVALIYSATYYRLTYSQLYKSITSLAAGLYHGLGVRKGDVVFILSPNSLLYPT 118

Query: 362 ICLSVLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSK 183
           ICL+VL IGAVITTANPL+T+ EI KQV DS A LAIS PE+ HKL ST VPI+LT   +
Sbjct: 119 ICLAVLTIGAVITTANPLHTEGEINKQVHDSGAKLAISCPEQIHKLYSTGVPILLTS-KE 177

Query: 182 ECDQLSVEELIEKCEPLDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
           + +QLSVEELI+ C+ L+IPE K  QSD AAILYSSGTTGTSKGVVLTHSNFISV  LLK
Sbjct: 178 DSNQLSVEELIQNCQALEIPEVKSIQSDIAAILYSSGTTGTSKGVVLTHSNFISVFRLLK 237


>ref|XP_023751376.1| 4-coumarate--CoA ligase 1-like [Lactuca sativa]
 gb|PLY94799.1| hypothetical protein LSAT_2X103361 [Lactuca sativa]
          Length = 565

 Score =  295 bits (754), Expect = 3e-93
 Identities = 152/219 (69%), Positives = 173/219 (78%)
 Frame = -3

Query: 659 SGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPDHAQTRVALIDSATGHRLT 480
           SGFD QT IYHSLL L  + KIP+ P LD AT+VLSQFP  D A++RVALID AT  R T
Sbjct: 22  SGFDPQTGIYHSLLQLGTNNKIPTTPYLDTATFVLSQFPKSDQAESRVALIDLATNQRAT 81

Query: 479 YAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICLSVLFIGAVITTANPLNTK 300
           Y QL  SI SLA GL+HGLG++KGDVVF+LSPNS LYP ICL+V  IG V+TTANPLNTK
Sbjct: 82  YGQLKRSIYSLATGLHHGLGIRKGDVVFLLSPNSILYPTICLAVFLIGGVLTTANPLNTK 141

Query: 299 REIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSKECDQLSVEELIEKCEPLDIPE 120
            EI KQV DS A LA+ AP E HKLVST VPI+LT      D+LSVEELI+ CEP+++PE
Sbjct: 142 SEITKQVLDSGATLAVVAPGEEHKLVSTNVPILLTSRPSSGDELSVEELIDGCEPMELPE 201

Query: 119 NKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
            +  QSDTAAILYSSGTTG SKGVVLTH+NFISV+TLLK
Sbjct: 202 TRSAQSDTAAILYSSGTTGVSKGVVLTHANFISVMTLLK 240


>gb|KVI05000.1| AMP-binding, conserved site-containing protein [Cynara cardunculus
           var. scolymus]
          Length = 549

 Score =  290 bits (742), Expect = 1e-91
 Identities = 143/221 (64%), Positives = 180/221 (81%)
 Frame = -3

Query: 665 KKSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPDHAQTRVALIDSATGHR 486
           +K G+D QT IYHSL+ L    KIP++ +LDIAT+VLSQFP  D A++RVALIDSAT HR
Sbjct: 18  RKGGYDPQTGIYHSLIRLRGKYKIPTRAHLDIATFVLSQFPRLDQAESRVALIDSATNHR 77

Query: 485 LTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICLSVLFIGAVITTANPLN 306
           +TY QL  SI SLAAGLYHGLG++KGDVVFVLSPNS +YP ICL++L +G V+T +NP+N
Sbjct: 78  VTYGQLQRSIHSLAAGLYHGLGVRKGDVVFVLSPNSIIYPTICLAILLVGGVVTMSNPVN 137

Query: 305 TKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSKECDQLSVEELIEKCEPLDI 126
           T+ EI +QV DS A LAI+ PEE HKL+STKVP ++T  +   ++LSVEELI  CE +++
Sbjct: 138 TESEITEQVLDSGAKLAIADPEEMHKLLSTKVPTLVTTSNSSGNELSVEELINCCESMEL 197

Query: 125 PENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
           PE++P QSDTAAILYSSGTTGTSKGV+LTHSN +S++ LLK
Sbjct: 198 PEDRPTQSDTAAILYSSGTTGTSKGVILTHSNLVSMMMLLK 238


>ref|XP_024167799.1| 4-coumarate--CoA ligase-like 5 [Rosa chinensis]
          Length = 566

 Score =  290 bits (741), Expect = 3e-91
 Identities = 153/236 (64%), Positives = 184/236 (77%), Gaps = 4/236 (1%)
 Frame = -3

Query: 698 MESLPNPLLFDKKSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPDHAQTR 519
           ME L          GFDSQT +YHSL +L    KIP++PNLD AT+VLSQFP P  A++R
Sbjct: 3   MEELSGEYTGRTNGGFDSQTGVYHSLFNLGDRHKIPTRPNLDTATFVLSQFPHPHQAESR 62

Query: 518 VALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICLSVLFI 339
           VALIDSAT  R+TYAQLH SI SLA+GL+  LG++KGDVVFVLSPNS LYP ICL+V  +
Sbjct: 63  VALIDSATNQRVTYAQLHRSIQSLASGLHQALGVQKGDVVFVLSPNSLLYPTICLAVFSV 122

Query: 338 GAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILT-KPSKECD-QLS 165
           GA++TTANPLNT+ EI KQVRDS A LAI++PEE HKLV T VP I+T +PS   D  LS
Sbjct: 123 GAILTTANPLNTESEIAKQVRDSGAKLAIASPEEVHKLVPTGVPTIVTSRPSNGTDNSLS 182

Query: 164 VEELIEKCEPL--DIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
           +EELIE C+P+  ++   +P QSDTAAILYSSGTTGTSKGVVLTH+NFI+++TLLK
Sbjct: 183 IEELIESCDPIPTELALARPTQSDTAAILYSSGTTGTSKGVVLTHANFIAIMTLLK 238


>gb|OTG07932.1| putative AMP-dependent synthetase/ligase, AMP-binding enzyme
           C-terminal domain protein [Helianthus annuus]
          Length = 536

 Score =  288 bits (738), Expect = 4e-91
 Identities = 146/220 (66%), Positives = 174/220 (79%)
 Frame = -3

Query: 662 KSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPDHAQTRVALIDSATGHRL 483
           + GFDSQT IYHSL+ L    KIP++ NLD AT+V+SQFP  D A ++VA ID+ATGH++
Sbjct: 18  RGGFDSQTGIYHSLVQLGDGNKIPTKANLDTATFVMSQFPKLDQAVSKVAFIDTATGHQV 77

Query: 482 TYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICLSVLFIGAVITTANPLNT 303
           TY+QL  SI SLA GLYHGLG++KGDVVFVLSPNS LYP ICL+V  +G V+TTANPLNT
Sbjct: 78  TYSQLQRSIYSLATGLYHGLGVRKGDVVFVLSPNSILYPTICLAVFLVGGVLTTANPLNT 137

Query: 302 KREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSKECDQLSVEELIEKCEPLDIP 123
           K EI KQV DS A LAI AP E HKLVST VP +LT   +  D+LSV+ELI+ CE +++P
Sbjct: 138 KSEITKQVLDSGAKLAIVAPSEAHKLVSTNVPTLLTYRPENGDELSVQELIDCCEQMELP 197

Query: 122 ENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
           E +  QSD AAILYSSGTTG SKGV+LTHSNFIS +TLLK
Sbjct: 198 ETRSTQSDIAAILYSSGTTGVSKGVLLTHSNFISCMTLLK 237


>gb|PRQ56377.1| putative AMP-dependent synthetase/ligase, AMP-binding enzyme
           domain-containing protein [Rosa chinensis]
          Length = 609

 Score =  290 bits (743), Expect = 4e-91
 Identities = 154/241 (63%), Positives = 186/241 (77%), Gaps = 4/241 (1%)
 Frame = -3

Query: 713 ITEEEMESLPNPLLFDKKSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPD 534
           I+   ME L          GFDSQT +YHSL +L    KIP++PNLD AT+VLSQFP P 
Sbjct: 49  ISTMSMEELSGEYTGRTNGGFDSQTGVYHSLFNLGDRHKIPTRPNLDTATFVLSQFPHPH 108

Query: 533 HAQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICL 354
            A++RVALIDSAT  R+TYAQLH SI SLA+GL+  LG++KGDVVFVLSPNS LYP ICL
Sbjct: 109 QAESRVALIDSATNQRVTYAQLHRSIQSLASGLHQALGVQKGDVVFVLSPNSLLYPTICL 168

Query: 353 SVLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILT-KPSKEC 177
           +V  +GA++TTANPLNT+ EI KQVRDS A LAI++PEE HKLV T VP I+T +PS   
Sbjct: 169 AVFSVGAILTTANPLNTESEIAKQVRDSGAKLAIASPEEVHKLVPTGVPTIVTSRPSNGT 228

Query: 176 D-QLSVEELIEKCEPL--DIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLL 6
           D  LS+EELIE C+P+  ++   +P QSDTAAILYSSGTTGTSKGVVLTH+NFI+++TLL
Sbjct: 229 DNSLSIEELIESCDPIPTELALARPTQSDTAAILYSSGTTGTSKGVVLTHANFIAIMTLL 288

Query: 5   K 3
           K
Sbjct: 289 K 289


>ref|XP_015876656.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Ziziphus jujuba]
          Length = 572

 Score =  289 bits (739), Expect = 7e-91
 Identities = 159/245 (64%), Positives = 187/245 (76%), Gaps = 3/245 (1%)
 Frame = -3

Query: 728 STKMAITEEEMESLPNPLL--FDKKSGFDSQTKIYHSLLHL-TQDQKIPSQPNLDIATYV 558
           S + A T    E +P+     F K +GFDS T IY SL  L  Q  KIP++P+LD AT+V
Sbjct: 2   SAEEAWTSTTAEKIPDHHQPDFYKTAGFDSVTGIYRSLYELGEQYYKIPTRPDLDTATFV 61

Query: 557 LSQFPSPDHAQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNS 378
           LSQFPSP  A++RVALIDSATG +LTYAQLH SI SLA+GLYH LG++KGDVVF+LSPNS
Sbjct: 62  LSQFPSPHQAESRVALIDSATGRQLTYAQLHRSIRSLASGLYHALGVRKGDVVFLLSPNS 121

Query: 377 SLYPIICLSVLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIIL 198
            LYP ICL+VL +GAV+TTANPLNT+ E+ KQV DS A LAISAPEE HKL +T VPII 
Sbjct: 122 LLYPTICLAVLSVGAVLTTANPLNTESELAKQVTDSGAKLAISAPEELHKLAATGVPIIP 181

Query: 197 TKPSKECDQLSVEELIEKCEPLDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISV 18
           T    +   LSV ELIE C+ L++   +PNQSDTAAILYSSGTTGTSKGVVLTHSNFISV
Sbjct: 182 T--ISDDGSLSVVELIESCDDLELDHERPNQSDTAAILYSSGTTGTSKGVVLTHSNFISV 239

Query: 17  VTLLK 3
           + + K
Sbjct: 240 IRIFK 244


>ref|XP_021993469.1| 4-coumarate--CoA ligase-like 5 [Helianthus annuus]
          Length = 562

 Score =  288 bits (738), Expect = 7e-91
 Identities = 146/220 (66%), Positives = 174/220 (79%)
 Frame = -3

Query: 662 KSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPDHAQTRVALIDSATGHRL 483
           + GFDSQT IYHSL+ L    KIP++ NLD AT+V+SQFP  D A ++VA ID+ATGH++
Sbjct: 18  RGGFDSQTGIYHSLVQLGDGNKIPTKANLDTATFVMSQFPKLDQAVSKVAFIDTATGHQV 77

Query: 482 TYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICLSVLFIGAVITTANPLNT 303
           TY+QL  SI SLA GLYHGLG++KGDVVFVLSPNS LYP ICL+V  +G V+TTANPLNT
Sbjct: 78  TYSQLQRSIYSLATGLYHGLGVRKGDVVFVLSPNSILYPTICLAVFLVGGVLTTANPLNT 137

Query: 302 KREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSKECDQLSVEELIEKCEPLDIP 123
           K EI KQV DS A LAI AP E HKLVST VP +LT   +  D+LSV+ELI+ CE +++P
Sbjct: 138 KSEITKQVLDSGAKLAIVAPSEAHKLVSTNVPTLLTYRPENGDELSVQELIDCCEQMELP 197

Query: 122 ENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
           E +  QSD AAILYSSGTTG SKGV+LTHSNFIS +TLLK
Sbjct: 198 ETRSTQSDIAAILYSSGTTGVSKGVLLTHSNFISCMTLLK 237


>ref|XP_018848044.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Juglans regia]
          Length = 573

 Score =  288 bits (738), Expect = 1e-90
 Identities = 153/238 (64%), Positives = 186/238 (78%), Gaps = 2/238 (0%)
 Frame = -3

Query: 710 TEEEMESLPNPLLFDKKSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPDH 531
           T  E ES      FD+KSGFD +T IYHSL+ L + ++IPS+P+LD AT+VLSQFP    
Sbjct: 10  TTTEEESANPQRGFDEKSGFDPETGIYHSLVKLGEQKQIPSRPDLDTATFVLSQFPDSHK 69

Query: 530 AQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICLS 351
           +++++ALID+AT   +TYAQLH SI SLA+GLYH LG++KGDVVFVLSPNS LYP ICL+
Sbjct: 70  SESQIALIDAATNQHVTYAQLHRSIRSLASGLYHALGVRKGDVVFVLSPNSLLYPTICLA 129

Query: 350 VLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSKECD- 174
           V  +GAV+TTANPLNT+ EI KQVRDS A LAISAP E HKLV T VP +LT      D 
Sbjct: 130 VFSVGAVLTTANPLNTQAEIAKQVRDSGAKLAISAPAELHKLVPTGVPTVLTSGKSSNDG 189

Query: 173 QLSVEELIEKC-EPLDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
            LS+EELIE C +PL++P+    QSDTAAILYSSGTTGTSKGV+LTH+NFIS+VTLL+
Sbjct: 190 LLSIEELIECCDDPLELPQISRAQSDTAAILYSSGTTGTSKGVILTHANFISLVTLLQ 247


>gb|KVI09677.1| AMP-binding, conserved site-containing protein [Cynara cardunculus
           var. scolymus]
          Length = 534

 Score =  286 bits (731), Expect = 4e-90
 Identities = 144/219 (65%), Positives = 174/219 (79%)
 Frame = -3

Query: 659 SGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPDHAQTRVALIDSATGHRLT 480
           SGFDS+T IYHSLL L  ++ IP+ P LD AT+VLSQFP  D A++++ALIDS T  RLT
Sbjct: 17  SGFDSRTGIYHSLLQLGDNRNIPTTPYLDTATFVLSQFPQRDQAESKLALIDSVTNKRLT 76

Query: 479 YAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICLSVLFIGAVITTANPLNTK 300
           Y+QL  SI SLAAGL+HGLG+ KGDVVFVLSPNS LYP ICL++  +G V+TTANPLNT+
Sbjct: 77  YSQLQRSIYSLAAGLHHGLGVCKGDVVFVLSPNSILYPTICLAIFLVGGVLTTANPLNTE 136

Query: 299 REIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSKECDQLSVEELIEKCEPLDIPE 120
            EI KQV DS A LAI+ P E HKL STK+P++LT      D+LSVEELI+ C P+++PE
Sbjct: 137 SEITKQVLDSGAKLAIADPREVHKLASTKLPVLLTYRPSSGDELSVEELIDNCGPMELPE 196

Query: 119 NKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
            +  QSDTAAILYSSGTTG SKGV+LTH+N ISV+TLLK
Sbjct: 197 TQSTQSDTAAILYSSGTTGVSKGVILTHANIISVMTLLK 235


>ref|XP_016470340.1| PREDICTED: 4-coumarate--CoA ligase-like 4 isoform X2 [Nicotiana
           tabacum]
          Length = 489

 Score =  284 bits (726), Expect = 7e-90
 Identities = 148/238 (62%), Positives = 190/238 (79%), Gaps = 2/238 (0%)
 Frame = -3

Query: 710 TEEEMESLPNPLLFDKKSGFDSQTKIYHSLLHLTQDQKIPSQP-NLDIATYVLSQFPSPD 534
           T EE ES+     +D+K+G+DS+T IYHS+L L++  K+PS   +LD A +VLSQFPSP 
Sbjct: 10  TGEEEESM-----YDRKNGYDSKTGIYHSVLQLSEQHKMPSDNLDLDTAKFVLSQFPSPA 64

Query: 533 HAQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICL 354
            A++++ALIDSA   +LTYAQL  +I SLA GLYH LG++KGDVVFVLSPNS LYP ICL
Sbjct: 65  QAESQIALIDSANNQKLTYAQLRRAIASLATGLYHALGVRKGDVVFVLSPNSLLYPTICL 124

Query: 353 SVLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSK-EC 177
           +VL +GA+++ ANPLNT+ EI KQVR SRA LAISAPEE HKLV T VP +LT  SK + 
Sbjct: 125 AVLSVGAILSPANPLNTESEIYKQVRLSRAKLAISAPEEVHKLVRTGVPTLLTTRSKNDE 184

Query: 176 DQLSVEELIEKCEPLDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
           + +SVE+LIE C+PL++P+++P  SDTAAILYSSGTTG SKGVVLTH+NF++++ LLK
Sbjct: 185 NSVSVEDLIENCDPLEMPKDRPMLSDTAAILYSSGTTGVSKGVVLTHANFVAIMKLLK 242


>ref|XP_002271586.3| PREDICTED: 4-coumarate--CoA ligase-like 5 [Vitis vinifera]
 emb|CAN70408.1| hypothetical protein VITISV_021990 [Vitis vinifera]
          Length = 569

 Score =  286 bits (732), Expect = 7e-90
 Identities = 150/242 (61%), Positives = 191/242 (78%), Gaps = 7/242 (2%)
 Frame = -3

Query: 707 EEEMESLPNPLL------FDKKSGFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQF 546
           EEEM++     L      FD +SGFDS+T +Y SL+ L    ++P++P+LD AT+VLSQF
Sbjct: 4   EEEMDAAAEQRLGGSPPDFDGRSGFDSRTGVYSSLIKLGGQHRVPTRPDLDTATFVLSQF 63

Query: 545 PSPDHAQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYP 366
           P+PD A++RVALIDSATG R+TYA+L  SI  LA GLYHGLG++KGDVVF+L+PNS LYP
Sbjct: 64  PAPDCAESRVALIDSATGRRVTYAELRRSIRMLATGLYHGLGIRKGDVVFLLAPNSLLYP 123

Query: 365 IICLSVLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPS 186
            ICL+VL IGAV+TTANPLNT+ EI KQV DS A +AISAPEE HKL+ T VP +LT  +
Sbjct: 124 TICLAVLSIGAVLTTANPLNTQSEISKQVDDSGAKVAISAPEELHKLLQTGVPTLLTHRT 183

Query: 185 KECDQLSVEELIEKCEP-LDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTL 9
            + + LS+EELI+  +P LD+P  + +QSDTAAILYSSGTTGTSKGV+LTH+ FI+++TL
Sbjct: 184 CDENSLSIEELIDCSDPALDLPPAQMSQSDTAAILYSSGTTGTSKGVILTHAKFIAMMTL 243

Query: 8   LK 3
           LK
Sbjct: 244 LK 245


>ref|XP_019198798.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Ipomoea nil]
          Length = 599

 Score =  286 bits (731), Expect = 2e-89
 Identities = 143/219 (65%), Positives = 178/219 (81%), Gaps = 1/219 (0%)
 Frame = -3

Query: 656 GFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPDHAQTRVALIDSATGHRLTY 477
           GFDS+T IYHSLL L +  KIP+ P+LD AT+VLSQFP    A++RVAL+DSAT   +TY
Sbjct: 55  GFDSKTGIYHSLLQLDEHTKIPTSPDLDTATFVLSQFPDISRAESRVALVDSATNQSVTY 114

Query: 476 AQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICLSVLFIGAVITTANPLNTKR 297
           AQ+H SI SLA GL+HGLG++KGDVVFVLSPNS LYP++CL+VL +GA+++T+NPLNT+ 
Sbjct: 115 AQIHRSIASLATGLHHGLGVRKGDVVFVLSPNSLLYPVMCLAVLKVGAILSTSNPLNTQA 174

Query: 296 EIEKQVRDSRAILAISAPEETHKLVSTKVP-IILTKPSKECDQLSVEELIEKCEPLDIPE 120
           EI KQV  S A LAI+APEE  KLV T VP +  T+P  + + LS+EELIE CEP D+PE
Sbjct: 175 EIAKQVSISGAKLAIAAPEEVDKLVPTGVPTLYTTRPKDDANALSIEELIENCEPRDLPE 234

Query: 119 NKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
            +P+QSDTAAILYSSGTTG SKGVVLTH+N ++V+TLLK
Sbjct: 235 CRPSQSDTAAILYSSGTTGVSKGVVLTHANLVAVMTLLK 273


>ref|XP_009766303.1| PREDICTED: 4-coumarate--CoA ligase-like 4 [Nicotiana sylvestris]
 ref|XP_016470276.1| PREDICTED: 4-coumarate--CoA ligase-like 4 isoform X1 [Nicotiana
           tabacum]
          Length = 566

 Score =  284 bits (726), Expect = 5e-89
 Identities = 148/238 (62%), Positives = 190/238 (79%), Gaps = 2/238 (0%)
 Frame = -3

Query: 710 TEEEMESLPNPLLFDKKSGFDSQTKIYHSLLHLTQDQKIPSQP-NLDIATYVLSQFPSPD 534
           T EE ES+     +D+K+G+DS+T IYHS+L L++  K+PS   +LD A +VLSQFPSP 
Sbjct: 10  TGEEEESM-----YDRKNGYDSKTGIYHSVLQLSEQHKMPSDNLDLDTAKFVLSQFPSPA 64

Query: 533 HAQTRVALIDSATGHRLTYAQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICL 354
            A++++ALIDSA   +LTYAQL  +I SLA GLYH LG++KGDVVFVLSPNS LYP ICL
Sbjct: 65  QAESQIALIDSANNQKLTYAQLRRAIASLATGLYHALGVRKGDVVFVLSPNSLLYPTICL 124

Query: 353 SVLFIGAVITTANPLNTKREIEKQVRDSRAILAISAPEETHKLVSTKVPIILTKPSK-EC 177
           +VL +GA+++ ANPLNT+ EI KQVR SRA LAISAPEE HKLV T VP +LT  SK + 
Sbjct: 125 AVLSVGAILSPANPLNTESEIYKQVRLSRAKLAISAPEEVHKLVRTGVPTLLTTRSKNDE 184

Query: 176 DQLSVEELIEKCEPLDIPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
           + +SVE+LIE C+PL++P+++P  SDTAAILYSSGTTG SKGVVLTH+NF++++ LLK
Sbjct: 185 NSVSVEDLIENCDPLEMPKDRPMLSDTAAILYSSGTTGVSKGVVLTHANFVAIMKLLK 242


>ref|XP_004308531.1| PREDICTED: 4-coumarate--CoA ligase-like 5 [Fragaria vesca subsp.
           vesca]
          Length = 562

 Score =  283 bits (724), Expect = 9e-89
 Identities = 148/222 (66%), Positives = 179/222 (80%), Gaps = 4/222 (1%)
 Frame = -3

Query: 656 GFDSQTKIYHSLLHLTQDQKIPSQPNLDIATYVLSQFPSPDHAQTRVALIDSATGHRLTY 477
           GFD QT +YHS+ +L    KIP+ PNLD AT+VLSQFP P  A++RVALIDSAT  R+TY
Sbjct: 13  GFDPQTGVYHSIFNLGDRHKIPTTPNLDTATFVLSQFPHPHLAESRVALIDSATNQRVTY 72

Query: 476 AQLHHSITSLAAGLYHGLGLKKGDVVFVLSPNSSLYPIICLSVLFIGAVITTANPLNTKR 297
           AQLH SI SLA+GL+  LG++KGDVVFVLSPNS LYP ICL+V  +GA++TTANPLNT+ 
Sbjct: 73  AQLHRSIQSLASGLHQALGVQKGDVVFVLSPNSLLYPTICLAVFSVGAILTTANPLNTES 132

Query: 296 EIEKQVRDSRAILAISAPEETHKLVSTKVPIILT-KPSKECD-QLSVEELIEKCEPL--D 129
           EI KQVRDS A LAI++PEE HKLV T VP I+T +PS   D  LS+EELIE C+P+  +
Sbjct: 133 EIGKQVRDSGAKLAIASPEEVHKLVPTGVPTIVTSRPSNGTDNSLSIEELIESCDPIPTE 192

Query: 128 IPENKPNQSDTAAILYSSGTTGTSKGVVLTHSNFISVVTLLK 3
           +   +P QSDTAAILYSSGTTGTSKGVVLTH+NFI+++TLLK
Sbjct: 193 LALARPTQSDTAAILYSSGTTGTSKGVVLTHANFIAIMTLLK 234