BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00004119
         (787 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PIN12506.1| putative hydrolase related to dienelactone hydrol...   318   e-105
ref|XP_020548284.1| carboxymethylenebutenolidase homolog isoform...   316   e-105
ref|XP_011074966.1| carboxymethylenebutenolidase homolog isoform...   316   e-104
ref|XP_022875970.1| carboxymethylenebutenolidase homolog [Olea e...   293   3e-96
gb|KZV37316.1| carboxymethylenebutenolidase [Dorcoceras hygromet...   290   3e-95
ref|XP_024170424.1| carboxymethylenebutenolidase homolog [Rosa c...   270   8e-87
ref|XP_006473242.1| PREDICTED: carboxymethylenebutenolidase homo...   270   9e-87
dbj|GAY39609.1| hypothetical protein CUMW_045700 [Citrus unshiu]      269   1e-86
ref|XP_006434667.1| carboxymethylenebutenolidase homolog [Citrus...   269   2e-86
ref|XP_024021071.1| carboxymethylenebutenolidase homolog [Morus ...   269   2e-86
gb|POF00317.1| carboxymethylenebutenolidase like [Quercus suber]      266   3e-86
ref|XP_008219015.1| PREDICTED: carboxymethylenebutenolidase homo...   267   2e-85
gb|OAY58679.1| hypothetical protein MANES_02G198600 [Manihot esc...   266   2e-85
ref|XP_023920407.1| carboxymethylenebutenolidase homolog [Quercu...   266   3e-85
ref|XP_012848297.1| PREDICTED: carboxymethylenebutenolidase homo...   265   3e-85
gb|KDP26974.1| hypothetical protein JCGZ_22194 [Jatropha curcas]      266   4e-85
ref|XP_015902438.1| PREDICTED: carboxymethylenebutenolidase homo...   265   1e-84
ref|XP_015576379.1| PREDICTED: carboxymethylenebutenolidase homo...   264   1e-84
gb|POO00167.1| Dienelactone hydrolase [Trema orientalis]              263   2e-84
ref|XP_021603841.1| carboxymethylenebutenolidase homolog [Maniho...   266   2e-84

>gb|PIN12506.1| putative hydrolase related to dienelactone hydrolase [Handroanthus
           impetiginosus]
          Length = 310

 Score =  318 bits (814), Expect = e-105
 Identities = 160/209 (76%), Positives = 181/209 (86%)
 Frame = -3

Query: 785 KQMQCFAVLKKSNTLRVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVK 606
           + MQ FAV  KSN  R  CSQVKVE+SI+DEACELV+G+ELTI DE A+GIRA LFQAVK
Sbjct: 45  RPMQSFAV-GKSNRFRACCSQVKVEDSINDEACELVSGSELTIGDE-AEGIRACLFQAVK 102

Query: 605 NNNETGILLLSDILGFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWIS 426
           NNN TGILLLSDI GFEDS+TRDFAYRVACNGYNVLVPDL++G+ WT DRPNALLEQWI+
Sbjct: 103 NNNGTGILLLSDIFGFEDSSTRDFAYRVACNGYNVLVPDLFDGNSWTNDRPNALLEQWIT 162

Query: 425 KNGSESIAKTIFASAKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVS 246
           K+  E I++TIFASAKWMINEFA +GISK+LGIIGFC+GGGRV+DILAQD+D  FGSAVS
Sbjct: 163 KHNPERISETIFASAKWMINEFATVGISKKLGIIGFCHGGGRVLDILAQDKDVLFGSAVS 222

Query: 245 FYGTRIDSSVGANIKVPLLLITGEGSWFC 159
           FYGTRI+ S  +NIKVPLLLITGE    C
Sbjct: 223 FYGTRINPSTASNIKVPLLLITGENDALC 251



 Score = 79.0 bits (193), Expect = 2e-13
 Identities = 34/39 (87%), Positives = 37/39 (94%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           +GR HGF HRPQTPEEDADAE+AFMIMRNWLHDGLLV+S
Sbjct: 272 RGRGHGFAHRPQTPEEDADAEDAFMIMRNWLHDGLLVRS 310


>ref|XP_020548284.1| carboxymethylenebutenolidase homolog isoform X2 [Sesamum indicum]
          Length = 270

 Score =  316 bits (809), Expect = e-105
 Identities = 161/211 (76%), Positives = 177/211 (83%), Gaps = 3/211 (1%)
 Frame = -3

Query: 782 QMQCFAVLKKSNTLRVSCSQVKVEESIS---DEACELVNGTELTIEDEEADGIRASLFQA 612
           Q Q  + + KSN LRV C +VKVEES S   DEACELVNGTELTI D  ADGIRA LF A
Sbjct: 2   QRQWSSAVCKSNKLRVRCREVKVEESTSVHDDEACELVNGTELTIGDN-ADGIRAYLFTA 60

Query: 611 VKNNNETGILLLSDILGFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQW 432
           VKNNN TGILLLSDI GFEDSATRDFAYRVAC GYNVLVPDL+NGDPWTKDRPN+LL+QW
Sbjct: 61  VKNNNATGILLLSDIFGFEDSATRDFAYRVACTGYNVLVPDLFNGDPWTKDRPNSLLQQW 120

Query: 431 ISKNGSESIAKTIFASAKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSA 252
           I+K+  E IAKTIFASA WM+NE AA+GIS+++GIIGFCYGGGRVIDILAQDQ  YFG  
Sbjct: 121 ITKHQPERIAKTIFASANWMVNELAAVGISRKVGIIGFCYGGGRVIDILAQDQGDYFGCG 180

Query: 251 VSFYGTRIDSSVGANIKVPLLLITGEGSWFC 159
           VSFYGTRIDSSV ++IKVPLLLITG+    C
Sbjct: 181 VSFYGTRIDSSVASHIKVPLLLITGDDDGLC 211



 Score = 65.9 bits (159), Expect = 6e-09
 Identities = 28/39 (71%), Positives = 32/39 (82%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           QGR H F HRPQTPEED DAEEAF +MRNWL++ LL K+
Sbjct: 232 QGRGHSFAHRPQTPEEDKDAEEAFTMMRNWLYNNLLAKT 270


>ref|XP_011074966.1| carboxymethylenebutenolidase homolog isoform X1 [Sesamum indicum]
          Length = 340

 Score =  316 bits (809), Expect = e-104
 Identities = 161/211 (76%), Positives = 177/211 (83%), Gaps = 3/211 (1%)
 Frame = -3

Query: 782 QMQCFAVLKKSNTLRVSCSQVKVEESIS---DEACELVNGTELTIEDEEADGIRASLFQA 612
           Q Q  + + KSN LRV C +VKVEES S   DEACELVNGTELTI D  ADGIRA LF A
Sbjct: 72  QRQWSSAVCKSNKLRVRCREVKVEESTSVHDDEACELVNGTELTIGDN-ADGIRAYLFTA 130

Query: 611 VKNNNETGILLLSDILGFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQW 432
           VKNNN TGILLLSDI GFEDSATRDFAYRVAC GYNVLVPDL+NGDPWTKDRPN+LL+QW
Sbjct: 131 VKNNNATGILLLSDIFGFEDSATRDFAYRVACTGYNVLVPDLFNGDPWTKDRPNSLLQQW 190

Query: 431 ISKNGSESIAKTIFASAKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSA 252
           I+K+  E IAKTIFASA WM+NE AA+GIS+++GIIGFCYGGGRVIDILAQDQ  YFG  
Sbjct: 191 ITKHQPERIAKTIFASANWMVNELAAVGISRKVGIIGFCYGGGRVIDILAQDQGDYFGCG 250

Query: 251 VSFYGTRIDSSVGANIKVPLLLITGEGSWFC 159
           VSFYGTRIDSSV ++IKVPLLLITG+    C
Sbjct: 251 VSFYGTRIDSSVASHIKVPLLLITGDDDGLC 281



 Score = 65.9 bits (159), Expect = 1e-08
 Identities = 28/39 (71%), Positives = 32/39 (82%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           QGR H F HRPQTPEED DAEEAF +MRNWL++ LL K+
Sbjct: 302 QGRGHSFAHRPQTPEEDKDAEEAFTMMRNWLYNNLLAKT 340


>ref|XP_022875970.1| carboxymethylenebutenolidase homolog [Olea europaea var.
           sylvestris]
          Length = 308

 Score =  293 bits (751), Expect = 3e-96
 Identities = 140/199 (70%), Positives = 168/199 (84%)
 Frame = -3

Query: 755 KSNTLRVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLL 576
           K+N   VSCSQV+VEES+SDEACELVNGTEL I + + + I A LFQAVKNNN TGILLL
Sbjct: 52  KTNKFEVSCSQVEVEESLSDEACELVNGTELIIGEGD-ESISAHLFQAVKNNNATGILLL 110

Query: 575 SDILGFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKT 396
           SDI GFE+S+T+DFAYRVACNGYNVLVPDL++GDPWTKD+P + +EQW++K   + +AK 
Sbjct: 111 SDIYGFEESSTKDFAYRVACNGYNVLVPDLFHGDPWTKDKPMSYIEQWLAKQNRQRVAKD 170

Query: 395 IFASAKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSV 216
           +FASAKWM+NEFAA+GISK+LGIIGFC+GGGRV+DIL+ DQD  FG  VSFYGTRI+SS 
Sbjct: 171 VFASAKWMMNEFAAVGISKKLGIIGFCFGGGRVVDILSHDQDTCFGVGVSFYGTRIESSA 230

Query: 215 GANIKVPLLLITGEGSWFC 159
            +NIKVPLLLI G+    C
Sbjct: 231 ASNIKVPLLLIAGDSDPLC 249



 Score = 75.1 bits (183), Expect = 5e-12
 Identities = 32/39 (82%), Positives = 36/39 (92%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           +GRDHGF HRPQTPEED DAEEAF IMR+WLHDGLLV++
Sbjct: 270 RGRDHGFAHRPQTPEEDEDAEEAFKIMRDWLHDGLLVQN 308


>gb|KZV37316.1| carboxymethylenebutenolidase [Dorcoceras hygrometricum]
          Length = 265

 Score =  290 bits (741), Expect = 3e-95
 Identities = 143/199 (71%), Positives = 165/199 (82%)
 Frame = -3

Query: 755 KSNTLRVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLL 576
           KS+   V CS VKVEES  DEACELVNGTELTI +E A  I+A LF+AVKNNN TGILLL
Sbjct: 9   KSSKFEVRCSPVKVEESQVDEACELVNGTELTIGNE-AYYIQAYLFKAVKNNNNTGILLL 67

Query: 575 SDILGFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKT 396
           SDILGFEDSATRDFAYRVACNGYNVLVPDL++GDPW KDRP A  E+WIS+   +S+ K 
Sbjct: 68  SDILGFEDSATRDFAYRVACNGYNVLVPDLFSGDPWRKDRPMASFEEWISRQEPQSVVKD 127

Query: 395 IFASAKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSV 216
           +  SA+WM+NEFA  GIS +LGIIGFC+GGGRVID+LAQD+ +YFG+ VSFYGTRIDSSV
Sbjct: 128 VLTSAEWMLNEFATAGISNKLGIIGFCFGGGRVIDVLAQDKGSYFGTGVSFYGTRIDSSV 187

Query: 215 GANIKVPLLLITGEGSWFC 159
            +NIKVPLLLI+G+    C
Sbjct: 188 ASNIKVPLLLISGDNDALC 206



 Score = 70.5 bits (171), Expect = 1e-10
 Identities = 30/39 (76%), Positives = 35/39 (89%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           +GR HGF HRP+TPEEDADAEEAFM+MRNWL+D LLV +
Sbjct: 227 RGRGHGFGHRPETPEEDADAEEAFMVMRNWLNDSLLVNN 265


>ref|XP_024170424.1| carboxymethylenebutenolidase homolog [Rosa chinensis]
 gb|PRQ20343.1| putative 2-oxo-3-(5-oxofuran-2-ylidene)propanoate lactonase [Rosa
           chinensis]
          Length = 317

 Score =  270 bits (690), Expect = 8e-87
 Identities = 131/209 (62%), Positives = 163/209 (77%)
 Frame = -3

Query: 782 QMQCFAVLKKSNTLRVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKN 603
           Q  C    +K    RVSCS +KVEE I+DEACELV+G EL++ + E D I A LF+AVKN
Sbjct: 50  QRACDIGRRKLVGRRVSCSLLKVEEDINDEACELVSGIELSVGEGE-DSINAHLFKAVKN 108

Query: 602 NNETGILLLSDILGFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISK 423
           NN TGILLLSDI GFEDS+TR+FAYRVACNGYNVL+PDL+  DPWTKDRP  L ++WI K
Sbjct: 109 NNGTGILLLSDIFGFEDSSTREFAYRVACNGYNVLLPDLFREDPWTKDRPKILFDEWIMK 168

Query: 422 NGSESIAKTIFASAKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSF 243
              + +AK I  SAKWM++EF A GISK+LG++GFC+GGG+VID+LA+DQ AYFG  VSF
Sbjct: 169 QEPQRVAKDISTSAKWMVDEFLAAGISKKLGLVGFCFGGGKVIDVLARDQGAYFGIGVSF 228

Query: 242 YGTRIDSSVGANIKVPLLLITGEGSWFCS 156
           YGTR+D SV ++IKVP+L I+G+    C+
Sbjct: 229 YGTRMDLSVASDIKVPVLFISGDNDPLCA 257



 Score = 62.0 bits (149), Expect = 2e-07
 Identities = 26/36 (72%), Positives = 31/36 (86%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLL 74
           +GR HGF HRPQ+ EED DAE+AF IMRNWL+DGL+
Sbjct: 278 KGRGHGFAHRPQSAEEDEDAEKAFTIMRNWLNDGLV 313


>ref|XP_006473242.1| PREDICTED: carboxymethylenebutenolidase homolog [Citrus sinensis]
          Length = 309

 Score =  270 bits (689), Expect = 9e-87
 Identities = 126/195 (64%), Positives = 156/195 (80%), Gaps = 1/195 (0%)
 Frame = -3

Query: 740 RVSCSQVKVEES-ISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLLSDIL 564
           +VSCS V+VEE  + DE CELVNG EL+I +E  D I+A LF+ VKNNN TG+LLLSDI 
Sbjct: 55  KVSCSLVEVEEDGLDDETCELVNGVELSIGEEADDAIQAYLFKPVKNNNGTGLLLLSDIF 114

Query: 563 GFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKTIFAS 384
           GF+DSATRDFAYRVACNGYNVLVPDL+ GDPW+K+RP A+ E+WI+K   E I+K I+ S
Sbjct: 115 GFQDSATRDFAYRVACNGYNVLVPDLFRGDPWSKERPKAMFEEWIAKQDFERISKDIYTS 174

Query: 383 AKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSVGANI 204
            KWM++EF A GISK+LGI+GFC+GGGRVID+LAQDQ   FG  VSFYGTRID S+ +N+
Sbjct: 175 TKWMVDEFMAAGISKKLGIVGFCFGGGRVIDVLAQDQGGCFGIGVSFYGTRIDQSLASNV 234

Query: 203 KVPLLLITGEGSWFC 159
            VP+L ++G+    C
Sbjct: 235 NVPVLFVSGDNDPLC 249



 Score = 64.3 bits (155), Expect = 3e-08
 Identities = 26/39 (66%), Positives = 32/39 (82%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           +GR HGF HRP +PEED DAE+AF  MRNWLHDGL++ +
Sbjct: 271 KGRGHGFAHRPSSPEEDEDAEQAFTEMRNWLHDGLVMNN 309


>dbj|GAY39609.1| hypothetical protein CUMW_045700 [Citrus unshiu]
          Length = 309

 Score =  269 bits (688), Expect = 1e-86
 Identities = 125/196 (63%), Positives = 157/196 (80%), Gaps = 1/196 (0%)
 Frame = -3

Query: 740 RVSCSQVKVEES-ISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLLSDIL 564
           +VSCS V+VEE  + DE CELVNG EL+I +E  D I+A LF+ VKNNN TG+LLLSDI 
Sbjct: 55  KVSCSLVEVEEDGLDDETCELVNGVELSIGEEADDAIQAYLFKPVKNNNGTGLLLLSDIF 114

Query: 563 GFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKTIFAS 384
           GF+DSATRDFAYRVACNGYNVLVPDL+ GDPW+K+RP A+ E+W++K   E I+K I+ S
Sbjct: 115 GFQDSATRDFAYRVACNGYNVLVPDLFRGDPWSKERPKAMFEEWLAKQDFERISKDIYTS 174

Query: 383 AKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSVGANI 204
            KWM++EF A GISK+LGI+GFC+GGGRVID+LAQDQ   FG  VSFYGTRID S+ +N+
Sbjct: 175 TKWMVDEFMAAGISKKLGIVGFCFGGGRVIDVLAQDQGGCFGIGVSFYGTRIDQSLASNV 234

Query: 203 KVPLLLITGEGSWFCS 156
            VP+L ++G+    C+
Sbjct: 235 NVPVLFVSGDNDPLCA 250



 Score = 64.3 bits (155), Expect = 3e-08
 Identities = 26/39 (66%), Positives = 32/39 (82%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           +GR HGF HRP +PEED DAE+AF  MRNWLHDGL++ +
Sbjct: 271 KGRGHGFAHRPSSPEEDEDAEQAFTEMRNWLHDGLVMNN 309


>ref|XP_006434667.1| carboxymethylenebutenolidase homolog [Citrus clementina]
 gb|ESR47907.1| hypothetical protein CICLE_v10001943mg [Citrus clementina]
          Length = 309

 Score =  269 bits (687), Expect = 2e-86
 Identities = 125/196 (63%), Positives = 157/196 (80%), Gaps = 1/196 (0%)
 Frame = -3

Query: 740 RVSCSQVKVEES-ISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLLSDIL 564
           +VSCS V+VEE  + DE CELVNG EL+I +E  D I+A LF+ VKNNN TG+LLLSDI 
Sbjct: 55  KVSCSLVEVEEDGLDDETCELVNGVELSIGEEADDAIQAYLFKPVKNNNGTGLLLLSDIF 114

Query: 563 GFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKTIFAS 384
           GF+DSATRDFAYRVACNGYNVLVPDL+ GDPW+K+RP A+ E+W++K   E I+K I+ S
Sbjct: 115 GFQDSATRDFAYRVACNGYNVLVPDLFRGDPWSKERPKAMFEEWLAKQDFERISKDIYTS 174

Query: 383 AKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSVGANI 204
            KWM++EF A GISK+LGI+GFC+GGGRVID+LAQDQ   FG  VSFYGTRID S+ +N+
Sbjct: 175 TKWMVDEFMAAGISKKLGIVGFCFGGGRVIDVLAQDQGGCFGIGVSFYGTRIDQSLASNV 234

Query: 203 KVPLLLITGEGSWFCS 156
            VP+L ++G+    C+
Sbjct: 235 NVPVLFVSGDNDPVCA 250



 Score = 64.3 bits (155), Expect = 3e-08
 Identities = 26/39 (66%), Positives = 32/39 (82%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           +GR HGF HRP +PEED DAE+AF  MRNWLHDGL++ +
Sbjct: 271 KGRGHGFAHRPSSPEEDEDAEQAFTEMRNWLHDGLVMNN 309


>ref|XP_024021071.1| carboxymethylenebutenolidase homolog [Morus notabilis]
          Length = 329

 Score =  269 bits (688), Expect = 2e-86
 Identities = 127/199 (63%), Positives = 157/199 (78%)
 Frame = -3

Query: 755 KSNTLRVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLL 576
           K+   RV+CS +KVE+ + D+ACELV+G EL+IE+ + D IRA LF+AVKNNN TGILLL
Sbjct: 71  KNIARRVNCSLLKVEDGLDDDACELVSGKELSIEEGD-DNIRAYLFKAVKNNNGTGILLL 129

Query: 575 SDILGFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKT 396
           SD+ GFEDSATRDFAYRVACNGYNVLVPDL+ GDPW K RP  + EQWI K   E +AK 
Sbjct: 130 SDVYGFEDSATRDFAYRVACNGYNVLVPDLFRGDPWAKHRPKDVFEQWIRKQDPERVAKD 189

Query: 395 IFASAKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSV 216
           I  S  WM+NEF A GISK+LG+IGFCYGGG+VID+L++DQ  YFG  VSFYGTRI+ ++
Sbjct: 190 IATSTNWMVNEFVAAGISKKLGLIGFCYGGGKVIDVLSRDQGTYFGIGVSFYGTRINPTL 249

Query: 215 GANIKVPLLLITGEGSWFC 159
            +N+KVP+L I+G+    C
Sbjct: 250 ASNVKVPVLFISGDNDPLC 268



 Score = 62.0 bits (149), Expect = 2e-07
 Identities = 24/36 (66%), Positives = 31/36 (86%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLL 74
           +GR HGFVHRP++PEED DAE+ F + RNWL+DGL+
Sbjct: 290 KGRGHGFVHRPESPEEDGDAEQVFFMTRNWLYDGLV 325


>gb|POF00317.1| carboxymethylenebutenolidase like [Quercus suber]
          Length = 259

 Score =  266 bits (681), Expect = 3e-86
 Identities = 127/194 (65%), Positives = 155/194 (79%)
 Frame = -3

Query: 740 RVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLLSDILG 561
           +VSCS +KVE+ I DEACELV+G EL+I  E AD I A LF+A+KNNN  GILLLSDI G
Sbjct: 7   KVSCSLLKVEDGIDDEACELVSGLELSI-GEGADSINACLFKAMKNNNGCGILLLSDIFG 65

Query: 560 FEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKTIFASA 381
           FEDS+TR+FAYRVACNGYNVLVPDL+ GDPW KDRP  + EQWI+    + +A  I AS 
Sbjct: 66  FEDSSTREFAYRVACNGYNVLVPDLFRGDPWAKDRPKIMFEQWIASQDPQRMANDIAAST 125

Query: 380 KWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSVGANIK 201
           KWM++EF A GISK+LG+IGFCYGGGRVI++LA+DQDA FG  VSFYGTR+D S+ ++IK
Sbjct: 126 KWMVDEFVAAGISKKLGVIGFCYGGGRVIEVLARDQDACFGIGVSFYGTRMDPSIASDIK 185

Query: 200 VPLLLITGEGSWFC 159
           VP+L I+G+    C
Sbjct: 186 VPVLFISGDNDPLC 199



 Score = 60.8 bits (146), Expect = 3e-07
 Identities = 25/37 (67%), Positives = 32/37 (86%)
 Frame = -1

Query: 175 RDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           R HGF HRP +PEED DAE+AFMIMRN+L+DGL++ +
Sbjct: 223 RGHGFAHRPASPEEDGDAEQAFMIMRNFLNDGLVINN 259


>ref|XP_008219015.1| PREDICTED: carboxymethylenebutenolidase homolog [Prunus mume]
          Length = 326

 Score =  267 bits (682), Expect = 2e-85
 Identities = 125/194 (64%), Positives = 158/194 (81%)
 Frame = -3

Query: 740 RVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLLSDILG 561
           +++CS +KVE+  +DE+CELV+G ELT+  E  D I A LF+AVKNNN TGILLLSDI G
Sbjct: 73  KITCSLLKVEDDSNDESCELVSGVELTV-GEGVDSINAYLFKAVKNNNGTGILLLSDIFG 131

Query: 560 FEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKTIFASA 381
           FEDS+TR+FAYRVACNGYNVL+PDL+ GDPWTKDRP  L ++WIS+   + IAK I  SA
Sbjct: 132 FEDSSTREFAYRVACNGYNVLLPDLFRGDPWTKDRPKNLFDKWISEQEPQRIAKDIATSA 191

Query: 380 KWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSVGANIK 201
           KWM++EF A GISK+ G+IGFC+GGG+VI++LAQDQ A FG+ VSFYGTRID+SV +N+K
Sbjct: 192 KWMVDEFLAAGISKKFGLIGFCFGGGKVIEVLAQDQGASFGTGVSFYGTRIDTSVASNVK 251

Query: 200 VPLLLITGEGSWFC 159
           VP+L I+G+    C
Sbjct: 252 VPILFISGDNDPLC 265



 Score = 61.6 bits (148), Expect = 3e-07
 Identities = 25/39 (64%), Positives = 33/39 (84%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           +GR HGF HRPQ+ EED DAE+AFM+M+NWL+DGL+  +
Sbjct: 287 KGRGHGFAHRPQSFEEDEDAEQAFMMMKNWLNDGLVANT 325


>gb|OAY58679.1| hypothetical protein MANES_02G198600 [Manihot esculenta]
          Length = 321

 Score =  266 bits (681), Expect = 2e-85
 Identities = 127/195 (65%), Positives = 156/195 (80%)
 Frame = -3

Query: 740 RVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLLSDILG 561
           RVSC+ +KVE+ I+DEACELVNG EL+I  E  D  RA LF+AVKNNN TGILLLSD+ G
Sbjct: 69  RVSCNLLKVEDDINDEACELVNGVELSI-GEGIDSFRAHLFKAVKNNNGTGILLLSDVFG 127

Query: 560 FEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKTIFASA 381
           FEDSATRDFAYRVACNGYNVLVPDL+ GDPW KD+P    EQW++K   + IA  I  +A
Sbjct: 128 FEDSATRDFAYRVACNGYNVLVPDLFRGDPWAKDQPMTTFEQWLAKQEPQRIAMDIATAA 187

Query: 380 KWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSVGANIK 201
           KWM +EF A+GISK+LG+IGFC+GGGR+I++L++DQ A FG  VSFYGTR+D SV +N+K
Sbjct: 188 KWMADEFLAVGISKKLGVIGFCFGGGRLIEVLSRDQGARFGIGVSFYGTRMDPSVASNVK 247

Query: 200 VPLLLITGEGSWFCS 156
           VP+L I+G+    CS
Sbjct: 248 VPVLFISGDNDPLCS 262



 Score = 70.1 bits (170), Expect = 3e-10
 Identities = 28/38 (73%), Positives = 34/38 (89%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVK 68
           +GR HGF HRP +PEED DAEEAF++MRNWLHDGL++K
Sbjct: 283 EGRGHGFAHRPNSPEEDRDAEEAFVLMRNWLHDGLVLK 320


>ref|XP_023920407.1| carboxymethylenebutenolidase homolog [Quercus suber]
          Length = 328

 Score =  266 bits (681), Expect = 3e-85
 Identities = 127/194 (65%), Positives = 155/194 (79%)
 Frame = -3

Query: 740 RVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLLSDILG 561
           +VSCS +KVE+ I DEACELV+G EL+I  E AD I A LF+A+KNNN  GILLLSDI G
Sbjct: 76  KVSCSLLKVEDGIDDEACELVSGLELSI-GEGADSINACLFKAMKNNNGCGILLLSDIFG 134

Query: 560 FEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKTIFASA 381
           FEDS+TR+FAYRVACNGYNVLVPDL+ GDPW KDRP  + EQWI+    + +A  I AS 
Sbjct: 135 FEDSSTREFAYRVACNGYNVLVPDLFRGDPWAKDRPKIMFEQWIASQDPQRMANDIAAST 194

Query: 380 KWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSVGANIK 201
           KWM++EF A GISK+LG+IGFCYGGGRVI++LA+DQDA FG  VSFYGTR+D S+ ++IK
Sbjct: 195 KWMVDEFVAAGISKKLGVIGFCYGGGRVIEVLARDQDACFGIGVSFYGTRMDPSIASDIK 254

Query: 200 VPLLLITGEGSWFC 159
           VP+L I+G+    C
Sbjct: 255 VPVLFISGDNDPLC 268



 Score = 60.8 bits (146), Expect = 5e-07
 Identities = 25/37 (67%), Positives = 32/37 (86%)
 Frame = -1

Query: 175 RDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           R HGF HRP +PEED DAE+AFMIMRN+L+DGL++ +
Sbjct: 292 RGHGFAHRPASPEEDGDAEQAFMIMRNFLNDGLVINN 328


>ref|XP_012848297.1| PREDICTED: carboxymethylenebutenolidase homolog [Erythranthe
           guttata]
 gb|EYU28218.1| hypothetical protein MIMGU_mgv1a010825mg [Erythranthe guttata]
          Length = 300

 Score =  265 bits (678), Expect = 3e-85
 Identities = 142/216 (65%), Positives = 163/216 (75%), Gaps = 8/216 (3%)
 Frame = -3

Query: 782 QMQCFAVLKKSNTLR-------VSCS-QVKVEESISDEACELVNGTELTIEDEEADGIRA 627
           Q Q FAV   S++ +       VSCS Q+K+EE   +E CELVNGTELTI     + +RA
Sbjct: 36  QKQPFAVHTSSSSSKNKLKSVIVSCSSQLKIEE---EETCELVNGTELTI----GENLRA 88

Query: 626 SLFQAVKNNNETGILLLSDILGFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNA 447
            LF AVKNNN TGILLLSDI GFEDS+T DFAYR+ACNGYNVLVPDL+NGD WTKDRP  
Sbjct: 89  HLFTAVKNNNATGILLLSDIFGFEDSSTTDFAYRLACNGYNVLVPDLFNGDSWTKDRP-- 146

Query: 446 LLEQWISKNGSESIAKTIFASAKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDA 267
            LE+WI+K+  E IAKT+ ASA+WM++EFA LGIS + GIIGFCYGGGRVIDILAQD+ A
Sbjct: 147 -LEEWITKHSPEKIAKTVSASAQWMLDEFAQLGISNKFGIIGFCYGGGRVIDILAQDKGA 205

Query: 266 YFGSAVSFYGTRIDSSVGANIKVPLLLITGEGSWFC 159
            FG  VSFYGTRIDSS+   I VPLLLITG     C
Sbjct: 206 LFGCGVSFYGTRIDSSIATQINVPLLLITGGNDPLC 241



 Score = 70.5 bits (171), Expect = 2e-10
 Identities = 31/37 (83%), Positives = 33/37 (89%)
 Frame = -1

Query: 175 RDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           R HGF HRPQTPEEDADAEEAF+IMRNWL DGLLV +
Sbjct: 264 RGHGFAHRPQTPEEDADAEEAFIIMRNWLSDGLLVNT 300


>gb|KDP26974.1| hypothetical protein JCGZ_22194 [Jatropha curcas]
          Length = 320

 Score =  266 bits (679), Expect = 4e-85
 Identities = 128/195 (65%), Positives = 154/195 (78%)
 Frame = -3

Query: 740 RVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLLSDILG 561
           +VSC+  KVE+ I+DEACELVNG EL+I D   D IRA LF+AVKNNN  GILLLSDI G
Sbjct: 71  KVSCNLFKVEDDINDEACELVNGMELSIGDG-VDTIRAYLFKAVKNNNGIGILLLSDIFG 129

Query: 560 FEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKTIFASA 381
           FEDS+TRDFAYRVACNGYNVLVPDL+ GDPW KDRP ++ EQWI K  +  I K I  +A
Sbjct: 130 FEDSSTRDFAYRVACNGYNVLVPDLFRGDPWGKDRPKSMFEQWIKKQEANRIVKDIATAA 189

Query: 380 KWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSVGANIK 201
           KWM +E  A GISK+LGIIGFCYGGGRV+D+L++DQ + F + VSFYGTRID S+ +N+K
Sbjct: 190 KWMADELLAAGISKKLGIIGFCYGGGRVVDVLSEDQGSCFSTGVSFYGTRIDPSIASNVK 249

Query: 200 VPLLLITGEGSWFCS 156
           VP+L I+G+    CS
Sbjct: 250 VPVLFISGDDDPLCS 264



 Score = 61.6 bits (148), Expect = 3e-07
 Identities = 25/36 (69%), Positives = 30/36 (83%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLL 74
           +GR H F HRP +PEED DAE+AF IMRNWL+DGL+
Sbjct: 285 EGRGHSFAHRPNSPEEDEDAEQAFAIMRNWLNDGLV 320


>ref|XP_015902438.1| PREDICTED: carboxymethylenebutenolidase homolog [Ziziphus jujuba]
 ref|XP_015902696.1| PREDICTED: carboxymethylenebutenolidase homolog [Ziziphus jujuba]
          Length = 318

 Score =  265 bits (676), Expect = 1e-84
 Identities = 126/199 (63%), Positives = 158/199 (79%)
 Frame = -3

Query: 755 KSNTLRVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLL 576
           K  T +V+C+ +KVE+ I+DEACELV G EL+I + + D I+A LF+AVKNNNETG+LLL
Sbjct: 54  KKVTSKVNCNLLKVEDDINDEACELVYGVELSIGEGD-DNIQAHLFKAVKNNNETGLLLL 112

Query: 575 SDILGFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKT 396
           SDI GFED+ATRDFAYRVACNGYNVL+PDL+ GDPW K+RP  L E+WI+K   + IAK 
Sbjct: 113 SDIFGFEDTATRDFAYRVACNGYNVLLPDLFRGDPWAKNRPKTLFEEWIAKQDPQRIAKD 172

Query: 395 IFASAKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSV 216
           I  S  WMI EF A GISK+LG+IGFC+GGG+VI++LA+DQ  +FG  VSFYGTR+D SV
Sbjct: 173 ISTSTNWMIEEFLAAGISKKLGLIGFCFGGGKVIEVLARDQGTHFGIGVSFYGTRMDLSV 232

Query: 215 GANIKVPLLLITGEGSWFC 159
            +NIKVP+L I+G+    C
Sbjct: 233 ASNIKVPVLFISGDDDPLC 251



 Score = 71.6 bits (174), Expect = 9e-11
 Identities = 29/39 (74%), Positives = 35/39 (89%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           QGR HGFVHRP++PEED DAE AF++MRNWLHDGL+ K+
Sbjct: 273 QGRGHGFVHRPESPEEDGDAERAFVMMRNWLHDGLVAKN 311


>ref|XP_015576379.1| PREDICTED: carboxymethylenebutenolidase homolog [Ricinus communis]
          Length = 310

 Score =  264 bits (675), Expect = 1e-84
 Identities = 128/195 (65%), Positives = 156/195 (80%)
 Frame = -3

Query: 740 RVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLLSDILG 561
           +V C+ +KVE+ I+DEACELVNG EL++  E  D I A LF AVKNNN TG+LLLSDI G
Sbjct: 58  QVFCNLLKVEDDINDEACELVNGVELSL-GEGVDTISAYLFTAVKNNNGTGVLLLSDIFG 116

Query: 560 FEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKTIFASA 381
           FEDS+TRDFAYR+ACNGYNVLVPDL+ GDPWTKDRP A+LEQWI+K   + +AK I  +A
Sbjct: 117 FEDSSTRDFAYRLACNGYNVLVPDLFCGDPWTKDRPKAMLEQWIAKQEPQRVAKDIATAA 176

Query: 380 KWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSVGANIK 201
           KWM +EF A GISK+LGIIGFC+GGGRVI++L++DQ A FG  VSFYGTR+D S+ + IK
Sbjct: 177 KWMADEFLAAGISKKLGIIGFCFGGGRVIEVLSRDQGACFGIGVSFYGTRMDPSLASEIK 236

Query: 200 VPLLLITGEGSWFCS 156
           VP+L I+G     CS
Sbjct: 237 VPVLFISGNSDPLCS 251



 Score = 67.4 bits (163), Expect = 3e-09
 Identities = 27/39 (69%), Positives = 35/39 (89%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVKS 65
           QGR HGF HRP +PEED DAE+AF+IMRNWL+DGL++++
Sbjct: 272 QGRGHGFAHRPNSPEEDKDAEQAFVIMRNWLYDGLVLEN 310


>gb|POO00167.1| Dienelactone hydrolase [Trema orientalis]
          Length = 276

 Score =  263 bits (671), Expect = 2e-84
 Identities = 122/199 (61%), Positives = 155/199 (77%)
 Frame = -3

Query: 755 KSNTLRVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLL 576
           K+   +++CS +KVE+ + DEACELVNG EL+I ++    + A LF+AVKNNN TGILLL
Sbjct: 21  KNTASKINCSLLKVEDGLDDEACELVNGIELSIGED----VDAYLFKAVKNNNGTGILLL 76

Query: 575 SDILGFEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKT 396
           SD+ GFEDS+TRDFAYRVACNGYNVLVPDL+ GDPW K RP A+ E+WI+K   E +A  
Sbjct: 77  SDVFGFEDSSTRDFAYRVACNGYNVLVPDLFRGDPWAKHRPKAMFEEWITKQEPERVAND 136

Query: 395 IFASAKWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSV 216
           I  S  WM+NEF A GISK+LG+IGFC+GGG+VI++LA+DQ AYFG  VSFYGTRID S+
Sbjct: 137 IAMSTNWMVNEFVAAGISKKLGLIGFCFGGGKVIEVLARDQGAYFGVGVSFYGTRIDPSL 196

Query: 215 GANIKVPLLLITGEGSWFC 159
            +N+K P+L I+G+    C
Sbjct: 197 ASNVKAPVLFISGDNYPLC 215



 Score = 66.6 bits (161), Expect = 4e-09
 Identities = 26/36 (72%), Positives = 33/36 (91%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLL 74
           +GR HGF HRP++PEEDADAE+AF++ RNWLHDGL+
Sbjct: 237 EGRGHGFAHRPESPEEDADAEQAFIMTRNWLHDGLV 272


>ref|XP_021603841.1| carboxymethylenebutenolidase homolog [Manihot esculenta]
          Length = 390

 Score =  266 bits (681), Expect = 2e-84
 Identities = 127/195 (65%), Positives = 156/195 (80%)
 Frame = -3

Query: 740 RVSCSQVKVEESISDEACELVNGTELTIEDEEADGIRASLFQAVKNNNETGILLLSDILG 561
           RVSC+ +KVE+ I+DEACELVNG EL+I  E  D  RA LF+AVKNNN TGILLLSD+ G
Sbjct: 138 RVSCNLLKVEDDINDEACELVNGVELSI-GEGIDSFRAHLFKAVKNNNGTGILLLSDVFG 196

Query: 560 FEDSATRDFAYRVACNGYNVLVPDLYNGDPWTKDRPNALLEQWISKNGSESIAKTIFASA 381
           FEDSATRDFAYRVACNGYNVLVPDL+ GDPW KD+P    EQW++K   + IA  I  +A
Sbjct: 197 FEDSATRDFAYRVACNGYNVLVPDLFRGDPWAKDQPMTTFEQWLAKQEPQRIAMDIATAA 256

Query: 380 KWMINEFAALGISKRLGIIGFCYGGGRVIDILAQDQDAYFGSAVSFYGTRIDSSVGANIK 201
           KWM +EF A+GISK+LG+IGFC+GGGR+I++L++DQ A FG  VSFYGTR+D SV +N+K
Sbjct: 257 KWMADEFLAVGISKKLGVIGFCFGGGRLIEVLSRDQGARFGIGVSFYGTRMDPSVASNVK 316

Query: 200 VPLLLITGEGSWFCS 156
           VP+L I+G+    CS
Sbjct: 317 VPVLFISGDNDPLCS 331



 Score = 70.1 bits (170), Expect = 4e-10
 Identities = 28/38 (73%), Positives = 34/38 (89%)
 Frame = -1

Query: 181 QGRDHGFVHRPQTPEEDADAEEAFMIMRNWLHDGLLVK 68
           +GR HGF HRP +PEED DAEEAF++MRNWLHDGL++K
Sbjct: 352 EGRGHGFAHRPNSPEEDRDAEEAFVLMRNWLHDGLVLK 389