BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00003347
         (1903 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_012839211.1| PREDICTED: putative tRNA pseudouridine synth...   867   0.0  
ref|XP_011099760.1| putative tRNA pseudouridine synthase Pus10 i...   829   0.0  
ref|XP_020547511.1| putative tRNA pseudouridine synthase Pus10 i...   820   0.0  
ref|XP_019186230.1| PREDICTED: putative tRNA pseudouridine synth...   652   0.0  
ref|XP_019186228.1| PREDICTED: putative tRNA pseudouridine synth...   640   0.0  
ref|XP_002284814.1| PREDICTED: putative tRNA pseudouridine synth...   634   0.0  
ref|XP_010320448.1| PREDICTED: putative tRNA pseudouridine synth...   629   0.0  
ref|XP_015074304.1| PREDICTED: putative tRNA pseudouridine synth...   627   0.0  
ref|XP_004238405.1| PREDICTED: putative tRNA pseudouridine synth...   627   0.0  
emb|CDP07328.1| unnamed protein product [Coffea canephora]            627   0.0  
ref|XP_015074305.1| PREDICTED: putative tRNA pseudouridine synth...   626   0.0  
emb|CBI18893.3| unnamed protein product, partial [Vitis vinifera]     627   0.0  
ref|XP_009595594.1| PREDICTED: putative tRNA pseudouridine synth...   625   0.0  
ref|XP_009595593.1| PREDICTED: putative tRNA pseudouridine synth...   625   0.0  
ref|XP_006342101.1| PREDICTED: putative tRNA pseudouridine synth...   620   0.0  
ref|XP_009775534.1| PREDICTED: putative tRNA pseudouridine synth...   619   0.0  
ref|XP_015161917.1| PREDICTED: putative tRNA pseudouridine synth...   619   0.0  
ref|XP_021641351.1| putative tRNA pseudouridine synthase Pus10 [...   619   0.0  
ref|XP_016432383.1| PREDICTED: putative tRNA pseudouridine synth...   619   0.0  
ref|XP_016432382.1| PREDICTED: putative tRNA pseudouridine synth...   618   0.0  

>ref|XP_012839211.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 [Erythranthe
            guttata]
          Length = 556

 Score =  867 bits (2240), Expect = 0.0
 Identities = 430/557 (77%), Positives = 481/557 (86%)
 Frame = +2

Query: 107  MADGVATAHNDDEEAHVSSATEANKDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLL 286
            M DG A  H  +E+AHVSSATE NKDDL FLRDAVR+LPPQ +KDLL NGVCTRCIFRL 
Sbjct: 1    MTDGCAPVHRSNEDAHVSSATETNKDDLEFLRDAVRSLPPQPVKDLLLNGVCTRCIFRLF 60

Query: 287  ILHEEICSRPSLSTPILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRV 466
             LH++ICS  S++T  LS++LHEL EH G+GAN+   ET DL DT +VQ+F+EEF  CRV
Sbjct: 61   CLHKQICSPSSMATRNLSSVLHELREH-GNGANNGVRETNDLTDTGYVQEFEEEF-VCRV 118

Query: 467  CLGILQFVHNDDKGMLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAV 646
            CLGILQFV++D KG+L++K+ A DFAA I DAVK++GH  D+F LEVSLPPIV   EQAV
Sbjct: 119  CLGILQFVYSDGKGVLVEKNNAGDFAATIEDAVKKEGHEIDNFCLEVSLPPIVVEKEQAV 178

Query: 647  WSYIKKKYGSQPWFREKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSD 826
            WSYI+KKY S+ WF EK+PSE I VKDFLKLSV NPLEKLLDVKSN SSFRI LTYTLSD
Sbjct: 179  WSYIEKKYSSESWFSEKFPSERIGVKDFLKLSVANPLEKLLDVKSNGSSFRICLTYTLSD 238

Query: 827  ASAMKQPIHESNGCYKRRKVDDIDFIKNSAGLHKVERHEPSRNSSIESGDDGTGFLAFPI 1006
            AS+  +P+ ESNGC KRRK+DD D +KN  GLH+VE+H    NSSIES D+G GFL FP+
Sbjct: 239  ASSKIEPLPESNGCSKRRKIDDSDVLKNCTGLHEVEKHNTVTNSSIESVDEGNGFLPFPL 298

Query: 1007 EKVIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPIC 1186
            EKV  CCSL  Q YKT IYVGGRYLKYSRNVSQ+RWIIDDERMGEAS+EEI+GG+ILPIC
Sbjct: 299  EKVNNCCSLISQCYKTSIYVGGRYLKYSRNVSQSRWIIDDERMGEASVEEIIGGSILPIC 358

Query: 1187 QGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVK 1366
            QGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSD LIKD+EMKIN+LE   V+VK
Sbjct: 359  QGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDVLIKDMEMKINSLERNLVKVK 418

Query: 1367 NLKFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLH 1546
            NLKFLGSQGW +MREGEAEKQKQYAALVWISRPLN+DDV +LSS KDMQILQKTP+RVLH
Sbjct: 419  NLKFLGSQGWVMMREGEAEKQKQYAALVWISRPLNDDDVLSLSSFKDMQILQKTPVRVLH 478

Query: 1547 RRSPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCR 1726
            RRSPL REKTIHWM++ER+AGSS YF+LHLCTQAGTYIKEFVHGDLGRT PSVGSILGCR
Sbjct: 479  RRSPLGREKTIHWMKVERVAGSSQYFMLHLCTQAGTYIKEFVHGDLGRTQPSVGSILGCR 538

Query: 1727 AEILQLDVTDVKMDCFQ 1777
            AEILQLDVTDVKMDCFQ
Sbjct: 539  AEILQLDVTDVKMDCFQ 555


>ref|XP_011099760.1| putative tRNA pseudouridine synthase Pus10 isoform X1 [Sesamum
            indicum]
          Length = 558

 Score =  829 bits (2142), Expect = 0.0
 Identities = 419/558 (75%), Positives = 460/558 (82%)
 Frame = +2

Query: 107  MADGVATAHNDDEEAHVSSATEANKDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLL 286
            MA   A A   +EEA  SS+ EA+ DDL  LRD+VR +P Q LKDLLSNGVC RCIFRL 
Sbjct: 1    MATTGAAADGINEEARFSSSVEADNDDLKLLRDSVRAVPAQPLKDLLSNGVCVRCIFRLF 60

Query: 287  ILHEEICSRPSLSTPILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRV 466
             LHE+ICSRPSL TPILS IL E+T  EGDG +SS  ETKDL D E +++ +EE + C V
Sbjct: 61   SLHEQICSRPSLVTPILSTILREVTGCEGDGTSSSPRETKDLNDLESLREVKEEIKVCSV 120

Query: 467  CLGILQFVHNDDKGMLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAV 646
            CLGILQF++NDD G L+KK  ASDFAAA+ + VKQ+GH  DSFSLEVSLP  V  NEQAV
Sbjct: 121  CLGILQFLYNDDNGTLVKKDSASDFAAAMEEVVKQEGHQSDSFSLEVSLPQDVVENEQAV 180

Query: 647  WSYIKKKYGSQPWFREKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSD 826
            W YI++KY S+ WF EK PSE  A KDFLKLSVTNPLE LL +K N SSFR+RLTYTLSD
Sbjct: 181  WLYIQEKYSSESWFLEKLPSECFATKDFLKLSVTNPLETLLGIKCNVSSFRVRLTYTLSD 240

Query: 827  ASAMKQPIHESNGCYKRRKVDDIDFIKNSAGLHKVERHEPSRNSSIESGDDGTGFLAFPI 1006
            AS   + I E NG  KRRK DD D  K   G H+VE H+ +R SS    D   G   FP+
Sbjct: 241  ASTRDRHIKEKNGFCKRRKTDDSDNFKKDGGFHRVEGHDLTRKSSTALEDGRVGLSKFPL 300

Query: 1007 EKVIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPIC 1186
            EKV QCCSLNFQ Y+T +YVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILP+C
Sbjct: 301  EKVNQCCSLNFQCYRTSVYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPMC 360

Query: 1187 QGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVK 1366
            QGD+YKFHAAGREDIDVRMLGTGRPFLVEIQNARQ PSD L+KDIE KIN+LESKFV+VK
Sbjct: 361  QGDTYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSDALVKDIENKINSLESKFVKVK 420

Query: 1367 NLKFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLH 1546
            NLK LG+QGWELMREGEAEKQKQYAALVWI RPLN+DDVQTL+S +DMQILQKTPIRVLH
Sbjct: 421  NLKVLGNQGWELMREGEAEKQKQYAALVWIPRPLNDDDVQTLASVRDMQILQKTPIRVLH 480

Query: 1547 RRSPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCR 1726
            RRSPLEREKTIHWMRIERIAGSS YFLLHLCTQAGTYIKEFVHGDLGRTHPS+GSIL CR
Sbjct: 481  RRSPLEREKTIHWMRIERIAGSSQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILKCR 540

Query: 1727 AEILQLDVTDVKMDCFQA 1780
            AEILQLDVTDVKMDCFQA
Sbjct: 541  AEILQLDVTDVKMDCFQA 558


>ref|XP_020547511.1| putative tRNA pseudouridine synthase Pus10 isoform X2 [Sesamum
            indicum]
          Length = 556

 Score =  820 bits (2119), Expect = 0.0
 Identities = 417/558 (74%), Positives = 458/558 (82%)
 Frame = +2

Query: 107  MADGVATAHNDDEEAHVSSATEANKDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLL 286
            MA   A A   +EEA  SS+ EA+ DDL  LRD+VR +P Q LKDLLSNGVC RCIFRL 
Sbjct: 1    MATTGAAADGINEEARFSSSVEADNDDLKLLRDSVRAVPAQPLKDLLSNGVCVRCIFRLF 60

Query: 287  ILHEEICSRPSLSTPILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRV 466
             LHE+ICSRPSL TPILS IL E+T  EGDG +SS  ETKDL D E +++ +EE + C V
Sbjct: 61   SLHEQICSRPSLVTPILSTILREVTGCEGDGTSSSPRETKDLNDLESLREVKEEIKVCSV 120

Query: 467  CLGILQFVHNDDKGMLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAV 646
            CLGILQF++NDD G L+KK  ASDFAAA+ + VKQ+GH  DSFSLEVSLP  V  NEQAV
Sbjct: 121  CLGILQFLYNDDNGTLVKKDSASDFAAAMEEVVKQEGHQSDSFSLEVSLPQDVVENEQAV 180

Query: 647  WSYIKKKYGSQPWFREKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSD 826
            W YI++KY S+ WF EK PSE  A KDFLKLSVTNPLE LL +K N SSFR+RLTYTLSD
Sbjct: 181  WLYIQEKYSSESWFLEKLPSECFATKDFLKLSVTNPLETLLGIKCNVSSFRVRLTYTLSD 240

Query: 827  ASAMKQPIHESNGCYKRRKVDDIDFIKNSAGLHKVERHEPSRNSSIESGDDGTGFLAFPI 1006
            AS   + I E NG  KRRK DD D  K   G H+VE H+ +R SS    D   G   FP+
Sbjct: 241  ASTRDRHIKEKNGFCKRRKTDDSDNFKKDGGFHRVEGHDLTRKSSTALEDGRVGLSKFPL 300

Query: 1007 EKVIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPIC 1186
            EKV QCCSLNFQ Y+T +YVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILP+C
Sbjct: 301  EKVNQCCSLNFQCYRTSVYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPMC 360

Query: 1187 QGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVK 1366
            QGD+YKFHAAGREDIDVRMLGTGRPFLVEIQNARQ PSD L+KDIE KIN+LESKFV+VK
Sbjct: 361  QGDTYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSDALVKDIENKINSLESKFVKVK 420

Query: 1367 NLKFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLH 1546
            NLK LG+QGWELMREGEAE  KQYAALVWI RPLN+DDVQTL+S +DMQILQKTPIRVLH
Sbjct: 421  NLKVLGNQGWELMREGEAE--KQYAALVWIPRPLNDDDVQTLASVRDMQILQKTPIRVLH 478

Query: 1547 RRSPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCR 1726
            RRSPLEREKTIHWMRIERIAGSS YFLLHLCTQAGTYIKEFVHGDLGRTHPS+GSIL CR
Sbjct: 479  RRSPLEREKTIHWMRIERIAGSSQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILKCR 538

Query: 1727 AEILQLDVTDVKMDCFQA 1780
            AEILQLDVTDVKMDCFQA
Sbjct: 539  AEILQLDVTDVKMDCFQA 556


>ref|XP_019186230.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Ipomoea nil]
          Length = 553

 Score =  652 bits (1681), Expect = 0.0
 Identities = 337/551 (61%), Positives = 412/551 (74%), Gaps = 5/551 (0%)
 Frame = +2

Query: 137  DDEEAHVSSATEA----NKDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEI 304
            D+ E  +SS   A    +KD L  + DAVR +P   +KDL S GVC RCIFR+L LHE I
Sbjct: 13   DNGETLISSTIIAEDDKDKDVLKLISDAVREVPANAIKDLWSIGVCARCIFRVLGLHELI 72

Query: 305  CSRPSLSTPILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQ 484
            CS PSLS  +L  +L  +    GDG +SSA       +  ++Q+   E   C++CLG+LQ
Sbjct: 73   CSHPSLSYSVLITVLGVVRIEGGDGVDSSAKH-----ELLNIQELTTEANVCKICLGVLQ 127

Query: 485  FVHNDDKGMLMKKHRASDFAAAIGDAVKQDGHN-FDSFSLEVSLPPIVDVNEQAVWSYIK 661
            FV +D+ G L+KK  A DFA+ I + VKQ  H+   SFSLEVSLPP+V  N+QA+W Y++
Sbjct: 128  FVFSDENGTLLKKDSAYDFASKIAELVKQQQHHQIGSFSLEVSLPPLVTENDQAIWLYVQ 187

Query: 662  KKYGSQPWFREKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMK 841
            KKY S+ WF++K  S+  + KD LKL V  PL+ LL +K  ES+F IRLTY+    S  +
Sbjct: 188  KKYESELWFQQKAASQCFSTKDVLKLLVIKPLQTLLGLKHMESTFHIRLTYS---GSLRQ 244

Query: 842  QPIHESNGCYKRRKVDDIDFIKNSAGLHKVERHEPSRNSSIESGDDGTGFLAFPIEKVIQ 1021
            Q  +E N   KRRK D       S  + KVE    +R SS++S  D   ++   IEKVIQ
Sbjct: 245  QNANERNVGNKRRKTDSA--YTQSVDIQKVEGEAFTRTSSLKSQKDCFDYVKLLIEKVIQ 302

Query: 1022 CCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPICQGDSY 1201
             C L  Q Y+ PIY+GGRYLKYSRNVSQTRW+IDDERMGEAS+EEI+GG ILP+CQGD Y
Sbjct: 303  PCHLTTQCYRNPIYIGGRYLKYSRNVSQTRWVIDDERMGEASVEEILGGCILPLCQGDGY 362

Query: 1202 KFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVKNLKFL 1381
            KFHAAGREDIDVRMLGTGRPFLVEIQNARQ PS+  IKDIE+KIN+LE++ V+V+NLK L
Sbjct: 363  KFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEGFIKDIEIKINSLENQLVKVRNLKSL 422

Query: 1382 GSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLHRRSPL 1561
            GSQGW ++ EGEAEKQKQY+ALVWISR +NN+D+QT+SS KDM+I+QKTPIRVLHRRSP+
Sbjct: 423  GSQGWAMINEGEAEKQKQYSALVWISRDVNNEDLQTISSVKDMKIIQKTPIRVLHRRSPM 482

Query: 1562 EREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAEILQ 1741
            EREK IHWM++ER+ GSS YFLLHLCTQAGTYIKEFVHGDLGRT+PS+GS+LGCRAEILQ
Sbjct: 483  EREKIIHWMKLERLEGSSQYFLLHLCTQAGTYIKEFVHGDLGRTNPSIGSMLGCRAEILQ 542

Query: 1742 LDVTDVKMDCF 1774
            LDVTDVKMDCF
Sbjct: 543  LDVTDVKMDCF 553


>ref|XP_019186228.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Ipomoea nil]
          Length = 556

 Score =  640 bits (1651), Expect = 0.0
 Identities = 335/554 (60%), Positives = 409/554 (73%), Gaps = 8/554 (1%)
 Frame = +2

Query: 137  DDEEAHVSSATEA----NKDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEI 304
            D+ E  +SS   A    +KD L  + DAVR +P   +KDL S GVC RCIFR+L LHE I
Sbjct: 13   DNGETLISSTIIAEDDKDKDVLKLISDAVREVPANAIKDLWSIGVCARCIFRVLGLHELI 72

Query: 305  CSRPSLSTPILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQ 484
            CS PSLS  +L  +L  +    GDG +SSA       +  ++Q+   E   C++CLG+LQ
Sbjct: 73   CSHPSLSYSVLITVLGVVRIEGGDGVDSSAKH-----ELLNIQELTTEANVCKICLGVLQ 127

Query: 485  FVHNDDKGMLMKKHRASDFAAAIGDAVKQDGHN-FDSFSLEVSLPPIVDVNEQAVWSYIK 661
            FV +D+ G L+KK  A DFA+ I + VKQ  H+   SFSLEVSLPP+V  N+QA+W Y++
Sbjct: 128  FVFSDENGTLLKKDSAYDFASKIAELVKQQQHHQIGSFSLEVSLPPLVTENDQAIWLYVQ 187

Query: 662  KKYGSQPWFREKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMK 841
            KKY S+ WF++K  S+  + KD LKL V  PL+ LL +K  ES+F IRLTY+    S  +
Sbjct: 188  KKYESELWFQQKAASQCFSTKDVLKLLVIKPLQTLLGLKHMESTFHIRLTYS---GSLRQ 244

Query: 842  QPIHESNGCYKRRKVDDIDFIKNSAGLHKVERHEPSRNSSIESGDDGTGFLAFPIEKVIQ 1021
            Q  +E N   KRRK D       S  + KVE    +R SS++S  D   ++   IEKVIQ
Sbjct: 245  QNANERNVGNKRRKTDSA--YTQSVDIQKVEGEAFTRTSSLKSQKDCFDYVKLLIEKVIQ 302

Query: 1022 CCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEE---IVGGNILPICQG 1192
             C L  Q Y+ PIY+GGRYLKYSRNVSQTRW+IDDERMGEAS+E      GG ILP+CQG
Sbjct: 303  PCHLTTQCYRNPIYIGGRYLKYSRNVSQTRWVIDDERMGEASVEVPQFSSGGCILPLCQG 362

Query: 1193 DSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVKNL 1372
            D YKFHAAGREDIDVRMLGTGRPFLVEIQNARQ PS+  IKDIE+KIN+LE++ V+V+NL
Sbjct: 363  DGYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEGFIKDIEIKINSLENQLVKVRNL 422

Query: 1373 KFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLHRR 1552
            K LGSQGW ++ EGEAEKQKQY+ALVWISR +NN+D+QT+SS KDM+I+QKTPIRVLHRR
Sbjct: 423  KSLGSQGWAMINEGEAEKQKQYSALVWISRDVNNEDLQTISSVKDMKIIQKTPIRVLHRR 482

Query: 1553 SPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAE 1732
            SP+EREK IHWM++ER+ GSS YFLLHLCTQAGTYIKEFVHGDLGRT+PS+GS+LGCRAE
Sbjct: 483  SPMEREKIIHWMKLERLEGSSQYFLLHLCTQAGTYIKEFVHGDLGRTNPSIGSMLGCRAE 542

Query: 1733 ILQLDVTDVKMDCF 1774
            ILQLDVTDVKMDCF
Sbjct: 543  ILQLDVTDVKMDCF 556


>ref|XP_002284814.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Vitis vinifera]
          Length = 578

 Score =  634 bits (1635), Expect = 0.0
 Identities = 344/580 (59%), Positives = 413/580 (71%), Gaps = 32/580 (5%)
 Frame = +2

Query: 131  HNDDEEAH----------VSSATE-----ANKDDLGFLRDAVRTLPPQILKDLLSNGVCT 265
            H++ E+AH          VSSA E     A +DD+  L DAV  + P  +KDLLS G+C 
Sbjct: 2    HHETEDAHASNGHEAVAKVSSAAEDRRMIAGEDDMRILNDAVSKVSPHAVKDLLSLGICI 61

Query: 266  RCIFRLLILHEEICSRPSLSTPILSAILHELTE-----HEGDGANSSAGETKDLIDTEHV 430
             CIFRL  +   I S PSL   IL  ++ E T+     + G G N  +G           
Sbjct: 62   GCIFRLSGVRGHIFSCPSLLLSILHPVIGEPTDSSTHRYTGIGGNEDSGAAHSS------ 115

Query: 431  QDFQEEFRFCRVCLGILQFVHNDDKGMLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVS 610
            Q+ + E   C +CLG+LQF + DDK ML+KK  A + A +I + VK++GH FD+FSLEVS
Sbjct: 116  QESECEPVICSICLGVLQFTYFDDKKMLVKKDSADELAVSIANLVKKEGHEFDNFSLEVS 175

Query: 611  LPPIVDVNEQAVWSYIKKKYGSQPWFREKYPSESIAVKDFLKLSVTNPLEKLLDVKSNES 790
            +PP++  NE  V  Y+KKKYGS+ WF+EK+ SE I+ KD LKLS+ NPLEKLLDVKS+ S
Sbjct: 176  IPPVIAENEHVVLLYMKKKYGSELWFQEKFLSERISAKDALKLSLCNPLEKLLDVKSSGS 235

Query: 791  S-FRIRLTYTLSDASAMKQPIHE-SNGCYKRRKVDDIDF--IKNSA--GLHKVERHEPSR 952
            S FRIRLTY    AS   Q I E S GC +RR   +  F  +  S   G+   +      
Sbjct: 236  STFRIRLTYAQPRASGKNQNITERSQGCKRRRTGTENIFSTVHGSVVDGIECSDISTSEA 295

Query: 953  NSSIESGDDG------TGFLAFPIEKVIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRW 1114
            ++ I    DG      +    FP+EKV Q C L F  Y+T IY+GGRYLKYSRNVSQTRW
Sbjct: 296  DAGIRKPSDGLQDNENSDCFKFPLEKVNQPCHLAFLCYRTHIYIGGRYLKYSRNVSQTRW 355

Query: 1115 IIDDERMGEASIEEIVGGNILPICQGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQF 1294
            IIDDERMGEAS+EEI+GGNILP+C GD+YKFHAAGREDI+VRMLG+GRPFLVEIQN+R  
Sbjct: 356  IIDDERMGEASVEEIIGGNILPMCLGDNYKFHAAGREDINVRMLGSGRPFLVEIQNSRHV 415

Query: 1295 PSDELIKDIEMKINNLESKFVRVKNLKFLGSQGWELMREGEAEKQKQYAALVWISRPLNN 1474
            PS+ LIK+IE+KINNLE+K VRVKNLK + S GW LM+EGE EKQKQYAALVWI RPL +
Sbjct: 416  PSEALIKEIEVKINNLENKLVRVKNLKLVDSHGWSLMQEGEEEKQKQYAALVWIDRPLKD 475

Query: 1475 DDVQTLSSPKDMQILQKTPIRVLHRRSPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGT 1654
            +D+Q +SS KD +ILQ+TPIRVLHRRSPLEREK IHWM+IE+IAGSS YFLLHLCTQAGT
Sbjct: 476  EDMQAISSLKDKKILQRTPIRVLHRRSPLEREKIIHWMKIEKIAGSSQYFLLHLCTQAGT 535

Query: 1655 YIKEFVHGDLGRTHPSVGSILGCRAEILQLDVTDVKMDCF 1774
            YIKEFVHGDLGRTHPSVGSILGCRAEILQLDVT+VKMDCF
Sbjct: 536  YIKEFVHGDLGRTHPSVGSILGCRAEILQLDVTEVKMDCF 575


>ref|XP_010320448.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Solanum lycopersicum]
          Length = 560

 Score =  629 bits (1621), Expect = 0.0
 Identities = 331/558 (59%), Positives = 402/558 (72%), Gaps = 16/558 (2%)
 Frame = +2

Query: 152  HVSSATEANKDD-LGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLST 328
            H+SSA  A +++ L  LR+AV  LP   +KDL S G C RCIFRL  LHE +CS PSLS 
Sbjct: 15   HLSSAEAAAEEEKLRLLREAVHALPVSAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSI 74

Query: 329  PILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKG 508
                 IL ++  ++    N  +            Q+   E  FC++CLGILQF++ DD G
Sbjct: 75   SKWCDILEDVMRNKDGEFNEQSH-----------QERSRETNFCKICLGILQFLYYDDTG 123

Query: 509  MLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWF 688
             L+KK+ A DFA  I + VKQ     DSFSLEVSLP +V  N+QAVW Y+K KY  + WF
Sbjct: 124  TLLKKYSADDFAVVISELVKQQYQQIDSFSLEVSLPSVVTENDQAVWLYMKNKYRHELWF 183

Query: 689  REKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGC 868
            +EK   + I+ KD  KL V NPL++LL VKS +SSFR+RLTY+ SDA   +  + E NG 
Sbjct: 184  QEKAECQFISTKDIFKLLVINPLQRLLGVKSTQSSFRVRLTYSHSDALT-RDDVQEGNGG 242

Query: 869  YKRRKVDDIDF---IKNSAGLHKVERHEPSRNSSIESGDDGTGF------------LAFP 1003
             KRRK D   F   +K +     V  H  S +S+I  GD  T              L   
Sbjct: 243  NKRRKTDKAGFTGDLKTTG--ESVTPHVESFDSNIVDGDGLTKVCPKEAQDLSCNNLMAK 300

Query: 1004 IEKVIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPI 1183
            ++KV + CSL     ++  Y+GGRYLKYSRNVSQTRW+IDDERMGEAS+EEI+GG ILPI
Sbjct: 301  LDKVGKPCSLAIHCCRSSTYIGGRYLKYSRNVSQTRWVIDDERMGEASVEEIIGGAILPI 360

Query: 1184 CQGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRV 1363
             QGD+YKFHAAGREDIDVRMLGTGRPFLVEIQNARQ PS+ LIK+IE KIN+LES++VRV
Sbjct: 361  FQGDNYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEALIKEIEKKINSLESQYVRV 420

Query: 1364 KNLKFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVL 1543
            KNL+ +GSQGW+LMREGEAEKQKQYAALVWISRP++++D+QT+SS K++++ QKTPIRVL
Sbjct: 421  KNLRAVGSQGWDLMREGEAEKQKQYAALVWISRPVSDEDLQTISSLKELKVAQKTPIRVL 480

Query: 1544 HRRSPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGC 1723
            HRRSPLEREK IHWM++ER++GSS Y LLHLCTQAGTYIKEFVHGD GRT PS+GSILGC
Sbjct: 481  HRRSPLEREKIIHWMQVERVSGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSIGSILGC 540

Query: 1724 RAEILQLDVTDVKMDCFQ 1777
            R EILQLDVTDVKMDCFQ
Sbjct: 541  RTEILQLDVTDVKMDCFQ 558


>ref|XP_015074304.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Solanum pennellii]
          Length = 560

 Score =  627 bits (1618), Expect = 0.0
 Identities = 330/558 (59%), Positives = 401/558 (71%), Gaps = 16/558 (2%)
 Frame = +2

Query: 152  HVSSATEANKDD-LGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLST 328
            H+SSA  A +++ L  LR+AV  LP   +KDL S G C RCIFRL  LHE +CS PSLS 
Sbjct: 15   HLSSAEAAAEEEKLRLLREAVHALPVSAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSI 74

Query: 329  PILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKG 508
                 IL ++   +    N  +            Q+   E  FC++CLGILQF++ DD G
Sbjct: 75   SKWCDILEDVMRKKDGEFNEQSH-----------QERSRETNFCKICLGILQFLYYDDTG 123

Query: 509  MLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWF 688
             L+KK+ A DFA  I + VKQ     DSFSLEVSLP +V  N+QAVW Y+K KY  + WF
Sbjct: 124  TLLKKYSADDFAVVISELVKQQYQQIDSFSLEVSLPSVVTENDQAVWLYMKNKYRHELWF 183

Query: 689  REKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGC 868
            +EK   + I+ KD  KL V NPL++LL VKS +SSFR+RLTY+ SDA   +  + E NG 
Sbjct: 184  QEKAECQFISTKDIFKLLVINPLQRLLGVKSTQSSFRVRLTYSHSDALT-RDDVQEGNGG 242

Query: 869  YKRRKVDDIDF---IKNSAGLHKVERHEPSRNSSIESGDDGTGF------------LAFP 1003
             KRRK D   F   +K +     V  H  S +S+I  GD  T              L   
Sbjct: 243  NKRRKTDKAGFTGDLKTTG--ESVTPHVESFDSNIVDGDGLTKVCPKEAQDLSCNNLMAK 300

Query: 1004 IEKVIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPI 1183
            ++K+ + CSL     ++  Y+GGRYLKYSRNVSQTRW+IDDERMGEAS+EEI+GG ILPI
Sbjct: 301  LDKIGKPCSLAIHCCRSSTYIGGRYLKYSRNVSQTRWVIDDERMGEASVEEIIGGAILPI 360

Query: 1184 CQGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRV 1363
             QGD+YKFHAAGREDIDVRMLGTGRPFLVEIQNARQ PS+ LIK+IE KIN+LES++VRV
Sbjct: 361  FQGDNYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEALIKEIEKKINSLESQYVRV 420

Query: 1364 KNLKFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVL 1543
            KNL+ +GSQGW+LMREGEAEKQKQYAALVWISRP++++D+QT+SS K++++ QKTPIRVL
Sbjct: 421  KNLRAVGSQGWDLMREGEAEKQKQYAALVWISRPVSDEDLQTISSLKELKVAQKTPIRVL 480

Query: 1544 HRRSPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGC 1723
            HRRSPLEREK IHWM++ER++GSS Y LLHLCTQAGTYIKEFVHGD GRT PS+GSILGC
Sbjct: 481  HRRSPLEREKIIHWMQVERVSGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSIGSILGC 540

Query: 1724 RAEILQLDVTDVKMDCFQ 1777
            R EILQLDVTDVKMDCFQ
Sbjct: 541  RTEILQLDVTDVKMDCFQ 558


>ref|XP_004238405.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Solanum lycopersicum]
          Length = 557

 Score =  627 bits (1617), Expect = 0.0
 Identities = 329/555 (59%), Positives = 400/555 (72%), Gaps = 13/555 (2%)
 Frame = +2

Query: 152  HVSSATEANKDD-LGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLST 328
            H+SSA  A +++ L  LR+AV  LP   +KDL S G C RCIFRL  LHE +CS PSLS 
Sbjct: 15   HLSSAEAAAEEEKLRLLREAVHALPVSAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSI 74

Query: 329  PILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKG 508
                 IL ++  ++    N  +            Q+   E  FC++CLGILQF++ DD G
Sbjct: 75   SKWCDILEDVMRNKDGEFNEQSH-----------QERSRETNFCKICLGILQFLYYDDTG 123

Query: 509  MLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWF 688
             L+KK+ A DFA  I + VKQ     DSFSLEVSLP +V  N+QAVW Y+K KY  + WF
Sbjct: 124  TLLKKYSADDFAVVISELVKQQYQQIDSFSLEVSLPSVVTENDQAVWLYMKNKYRHELWF 183

Query: 689  REKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGC 868
            +EK   + I+ KD  KL V NPL++LL VKS +SSFR+RLTY+ SDA   +  + E NG 
Sbjct: 184  QEKAECQFISTKDIFKLLVINPLQRLLGVKSTQSSFRVRLTYSHSDALT-RDDVQEGNGG 242

Query: 869  YKRRKVDDIDFIKNSAGLHKVERHEPSRNSSIESGDDGTGF------------LAFPIEK 1012
             KRRK      +K +     V  H  S +S+I  GD  T              L   ++K
Sbjct: 243  NKRRKTGFTGDLKTTG--ESVTPHVESFDSNIVDGDGLTKVCPKEAQDLSCNNLMAKLDK 300

Query: 1013 VIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPICQG 1192
            V + CSL     ++  Y+GGRYLKYSRNVSQTRW+IDDERMGEAS+EEI+GG ILPI QG
Sbjct: 301  VGKPCSLAIHCCRSSTYIGGRYLKYSRNVSQTRWVIDDERMGEASVEEIIGGAILPIFQG 360

Query: 1193 DSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVKNL 1372
            D+YKFHAAGREDIDVRMLGTGRPFLVEIQNARQ PS+ LIK+IE KIN+LES++VRVKNL
Sbjct: 361  DNYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEALIKEIEKKINSLESQYVRVKNL 420

Query: 1373 KFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLHRR 1552
            + +GSQGW+LMREGEAEKQKQYAALVWISRP++++D+QT+SS K++++ QKTPIRVLHRR
Sbjct: 421  RAVGSQGWDLMREGEAEKQKQYAALVWISRPVSDEDLQTISSLKELKVAQKTPIRVLHRR 480

Query: 1553 SPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAE 1732
            SPLEREK IHWM++ER++GSS Y LLHLCTQAGTYIKEFVHGD GRT PS+GSILGCR E
Sbjct: 481  SPLEREKIIHWMQVERVSGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSIGSILGCRTE 540

Query: 1733 ILQLDVTDVKMDCFQ 1777
            ILQLDVTDVKMDCFQ
Sbjct: 541  ILQLDVTDVKMDCFQ 555


>emb|CDP07328.1| unnamed protein product [Coffea canephora]
          Length = 572

 Score =  627 bits (1617), Expect = 0.0
 Identities = 330/561 (58%), Positives = 411/561 (73%), Gaps = 16/561 (2%)
 Frame = +2

Query: 140  DEEAHVSSATEANK----DDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEIC 307
            DE   +S++  AN     ++L  L+DAV  LP   +KD  S G C RCIFRL  L ++I 
Sbjct: 13   DERPQISNSAAANVAEEVEELKLLQDAVYALPTHAVKDFFSLGACARCIFRLFGLCQKIS 72

Query: 308  SRPSLSTPILSAILHE-LTEHEGDGANSSAGETKDLIDTE-HVQDFQEEFRFCRVCLGIL 481
            S PSL T  ++A+L + +   + D   S + E KD    +   Q F  E   CR+CLGIL
Sbjct: 73   SFPSLPTATMNAMLEKNVLGRDRDDNCSGSAERKDSGSQQLSSQTFVLEHAVCRICLGIL 132

Query: 482  QFVHNDDKGMLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIK 661
             +V++D+K +L+KK  A +FA  + ++VKQ+    DSFSLEVSLPP+V  NEQ V S+++
Sbjct: 133  DYVYHDEKEILVKKDSAYEFAKIVAESVKQEHPQIDSFSLEVSLPPVVMDNEQVVRSFMR 192

Query: 662  KKYGSQPWFREKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMK 841
            KKYGS+ WF +K  ++ I++KD LKLS+ + LEKLL  KSN S+FRIRLTY  S+ S  K
Sbjct: 193  KKYGSEHWFLQKTLTDCISIKDALKLSILDTLEKLLGTKSNLSTFRIRLTYETSEESRRK 252

Query: 842  QPIHESNGCYKRR----------KVDDIDFIKNSAGLHKVERHEPSRNSSIESGDDGTGF 991
                E +GC +R+          +  +    KN A  H+ +++E +   + +    G+ +
Sbjct: 253  PDNEEIDGCKRRKTNMLYTADEKQASETSGSKNFAE-HENKQYECTEGFAKDLEGHGSDY 311

Query: 992  LAFPIEKVIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGN 1171
            L F +EK  Q   L F  Y+ PIY+GGRYLKYSRNVSQT W+I+DERMGEAS+EEI+GG+
Sbjct: 312  LKFQLEKESQPFGLVFLCYRCPIYIGGRYLKYSRNVSQTCWMIEDERMGEASVEEIIGGS 371

Query: 1172 ILPICQGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESK 1351
            ILP+CQGD YKFHAAGREDIDVRMLGTGRPFLVEIQNA Q PS+ELIK++EMKIN+ E+K
Sbjct: 372  ILPLCQGDGYKFHAAGREDIDVRMLGTGRPFLVEIQNAHQVPSEELIKEMEMKINSQENK 431

Query: 1352 FVRVKNLKFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTP 1531
             V VKNLK L ++GW LMREGEAEKQKQYAALVWISRP N+DD +TLSS ++MQILQKTP
Sbjct: 432  LVGVKNLKILDNKGWALMREGEAEKQKQYAALVWISRPFNDDDSKTLSSLQEMQILQKTP 491

Query: 1532 IRVLHRRSPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGS 1711
            IRVLHRRSPLEREK IHWM++E+IAGS  YFLLHLCTQAGTYIKEFVHGDLGRTHPS+GS
Sbjct: 492  IRVLHRRSPLEREKVIHWMKVEKIAGSCQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGS 551

Query: 1712 ILGCRAEILQLDVTDVKMDCF 1774
            ILGCRAEILQLDVTDVKMDCF
Sbjct: 552  ILGCRAEILQLDVTDVKMDCF 572


>ref|XP_015074305.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Solanum pennellii]
          Length = 557

 Score =  626 bits (1614), Expect = 0.0
 Identities = 328/555 (59%), Positives = 399/555 (71%), Gaps = 13/555 (2%)
 Frame = +2

Query: 152  HVSSATEANKDD-LGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLST 328
            H+SSA  A +++ L  LR+AV  LP   +KDL S G C RCIFRL  LHE +CS PSLS 
Sbjct: 15   HLSSAEAAAEEEKLRLLREAVHALPVSAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSI 74

Query: 329  PILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKG 508
                 IL ++   +    N  +            Q+   E  FC++CLGILQF++ DD G
Sbjct: 75   SKWCDILEDVMRKKDGEFNEQSH-----------QERSRETNFCKICLGILQFLYYDDTG 123

Query: 509  MLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWF 688
             L+KK+ A DFA  I + VKQ     DSFSLEVSLP +V  N+QAVW Y+K KY  + WF
Sbjct: 124  TLLKKYSADDFAVVISELVKQQYQQIDSFSLEVSLPSVVTENDQAVWLYMKNKYRHELWF 183

Query: 689  REKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGC 868
            +EK   + I+ KD  KL V NPL++LL VKS +SSFR+RLTY+ SDA   +  + E NG 
Sbjct: 184  QEKAECQFISTKDIFKLLVINPLQRLLGVKSTQSSFRVRLTYSHSDALT-RDDVQEGNGG 242

Query: 869  YKRRKVDDIDFIKNSAGLHKVERHEPSRNSSIESGDDGTGF------------LAFPIEK 1012
             KRRK      +K +     V  H  S +S+I  GD  T              L   ++K
Sbjct: 243  NKRRKTGFTGDLKTTG--ESVTPHVESFDSNIVDGDGLTKVCPKEAQDLSCNNLMAKLDK 300

Query: 1013 VIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPICQG 1192
            + + CSL     ++  Y+GGRYLKYSRNVSQTRW+IDDERMGEAS+EEI+GG ILPI QG
Sbjct: 301  IGKPCSLAIHCCRSSTYIGGRYLKYSRNVSQTRWVIDDERMGEASVEEIIGGAILPIFQG 360

Query: 1193 DSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVKNL 1372
            D+YKFHAAGREDIDVRMLGTGRPFLVEIQNARQ PS+ LIK+IE KIN+LES++VRVKNL
Sbjct: 361  DNYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEALIKEIEKKINSLESQYVRVKNL 420

Query: 1373 KFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLHRR 1552
            + +GSQGW+LMREGEAEKQKQYAALVWISRP++++D+QT+SS K++++ QKTPIRVLHRR
Sbjct: 421  RAVGSQGWDLMREGEAEKQKQYAALVWISRPVSDEDLQTISSLKELKVAQKTPIRVLHRR 480

Query: 1553 SPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAE 1732
            SPLEREK IHWM++ER++GSS Y LLHLCTQAGTYIKEFVHGD GRT PS+GSILGCR E
Sbjct: 481  SPLEREKIIHWMQVERVSGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSIGSILGCRTE 540

Query: 1733 ILQLDVTDVKMDCFQ 1777
            ILQLDVTDVKMDCFQ
Sbjct: 541  ILQLDVTDVKMDCFQ 555


>emb|CBI18893.3| unnamed protein product, partial [Vitis vinifera]
          Length = 591

 Score =  627 bits (1617), Expect = 0.0
 Identities = 342/575 (59%), Positives = 410/575 (71%), Gaps = 27/575 (4%)
 Frame = +2

Query: 131  HNDDEEAH----------VSSATE-----ANKDDLGFLRDAVRTLPPQILKDLLSNGVCT 265
            H++ E+AH          VSSA E     A +DD+  L DAV  + P  +KDLLS G+C 
Sbjct: 33   HHETEDAHASNGHEAVAKVSSAAEDRRMIAGEDDMRILNDAVSKVSPHAVKDLLSLGICI 92

Query: 266  RCIFRLLILHEEICSRPSLSTPILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQE 445
             CIFRL  +   I S PSL    L +ILH + +  G   +S   E + +I          
Sbjct: 93   GCIFRLSGVRGHIFSCPSL----LLSILHPVID-SGAAHSSQESECEPVI---------- 137

Query: 446  EFRFCRVCLGILQFVHNDDKGMLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIV 625
                C +CLG+LQF + DDK ML+KK  A + A +I + VK++GH FD+FSLEVS+PP++
Sbjct: 138  ----CSICLGVLQFTYFDDKKMLVKKDSADELAVSIANLVKKEGHEFDNFSLEVSIPPVI 193

Query: 626  DVNEQAVWSYIKKKYGSQPWFREKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESS-FRI 802
              NE  V  Y+KKKYGS+ WF+EK+ SE I+ KD LKLS+ NPLEKLLDVKS+ SS FRI
Sbjct: 194  AENEHVVLLYMKKKYGSELWFQEKFLSERISAKDALKLSLCNPLEKLLDVKSSGSSTFRI 253

Query: 803  RLTYTLSDASAMKQPIHE-SNGCYKRRKVDDIDF--IKNSA--GLHKVERHEPSRNSSIE 967
            RLTY    AS   Q I E S GC +RR   +  F  +  S   G+   +      ++ I 
Sbjct: 254  RLTYAQPRASGKNQNITERSQGCKRRRTGTENIFSTVHGSVVDGIECSDISTSEADAGIR 313

Query: 968  SGDDG------TGFLAFPIEKVIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDE 1129
               DG      +    FP+EKV Q C L F  Y+T IY+GGRYLKYSRNVSQTRWIIDDE
Sbjct: 314  KPSDGLQDNENSDCFKFPLEKVNQPCHLAFLCYRTHIYIGGRYLKYSRNVSQTRWIIDDE 373

Query: 1130 RMGEASIEEIVGGNILPICQGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDEL 1309
            RMGEAS+EEI+GGNILP+C GD+YKFHAAGREDI+VRMLG+GRPFLVEIQN+R  PS+ L
Sbjct: 374  RMGEASVEEIIGGNILPMCLGDNYKFHAAGREDINVRMLGSGRPFLVEIQNSRHVPSEAL 433

Query: 1310 IKDIEMKINNLESKFVRVKNLKFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQT 1489
            IK+IE+KINNLE+K VRVKNLK + S GW LM+EGE EKQKQYAALVWI RPL ++D+Q 
Sbjct: 434  IKEIEVKINNLENKLVRVKNLKLVDSHGWSLMQEGEEEKQKQYAALVWIDRPLKDEDMQA 493

Query: 1490 LSSPKDMQILQKTPIRVLHRRSPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEF 1669
            +SS KD +ILQ+TPIRVLHRRSPLEREK IHWM+IE+IAGSS YFLLHLCTQAGTYIKEF
Sbjct: 494  ISSLKDKKILQRTPIRVLHRRSPLEREKIIHWMKIEKIAGSSQYFLLHLCTQAGTYIKEF 553

Query: 1670 VHGDLGRTHPSVGSILGCRAEILQLDVTDVKMDCF 1774
            VHGDLGRTHPSVGSILGCRAEILQLDVT+VKMDCF
Sbjct: 554  VHGDLGRTHPSVGSILGCRAEILQLDVTEVKMDCF 588


>ref|XP_009595594.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Nicotiana tomentosiformis]
          Length = 564

 Score =  625 bits (1613), Expect = 0.0
 Identities = 332/547 (60%), Positives = 396/547 (72%), Gaps = 13/547 (2%)
 Frame = +2

Query: 179  KDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLSTPILSAILHEL 358
            ++ L  LRDAV  LP   +KDL S G C RCIFRL  LHE +CS PSLS      IL ++
Sbjct: 21   EEKLRLLRDAVNELPVNAVKDLWSIGACARCIFRLFGLHELMCSHPSLSISKWYDILADV 80

Query: 359  TEHEG-DGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKGMLMKKHRAS 535
               E  D A+    + +  I   H Q+  +E  FC++CLGILQF++NDD G L+KK  A 
Sbjct: 81   MRKEDVDTADHPQKDGEFSIKQLH-QEPLKETNFCKICLGILQFLYNDDSGTLLKKDSAD 139

Query: 536  DFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWFREKYPSESI 715
            DFA A+ + VKQ  H  DSFSLE SLP  +  N+QAVW Y+K +Y  + WF+EK   + I
Sbjct: 140  DFALAVSEPVKQQHHQIDSFSLEFSLPSALTENDQAVWLYMKNRYHHELWFQEKAEYQFI 199

Query: 716  AVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGCYKRRKVDDI 895
              KD LKL VTNPL+ LL VKS++ S RIRLTY+ SDA   +  I E NG  KRRK    
Sbjct: 200  CTKDILKLLVTNPLQSLLGVKSSQGSLRIRLTYSHSDALT-RDDIQERNGGNKRRKTGFT 258

Query: 896  DFIKNSAGLHKVERHEPSRNSSIESGDDGT------------GFLAFPIEKVIQCCSLNF 1039
              +K +     V  H  S ++ I  GD  T             +L   + KV + C L  
Sbjct: 259  GDLKTTE--ESVTPHVESFDNHIGDGDGLTKVCPKDSQDLSCSYLMAQLNKVSKPCCLTI 316

Query: 1040 QFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPICQGDSYKFHAAG 1219
               ++PI++GGRYLKYSRNVSQT W+IDDERMGEAS+EEIVGG ILPI QGD+YKFHAAG
Sbjct: 317  HCCRSPIFIGGRYLKYSRNVSQTPWVIDDERMGEASVEEIVGGAILPIFQGDNYKFHAAG 376

Query: 1220 REDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVKNLKFLGSQGWE 1399
            REDIDVRMLGTGRPFLVEIQNAR+ PS+ LIK+IE KIN+LES+ VRVKNL+ +GSQGW+
Sbjct: 377  REDIDVRMLGTGRPFLVEIQNARKVPSEVLIKEIERKINSLESQLVRVKNLRAVGSQGWD 436

Query: 1400 LMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLHRRSPLEREKTI 1579
            LMREGEAEKQKQY ALVWISRP+++DD+QT+SS K++++ QKTPIRVLHRRSPLEREK I
Sbjct: 437  LMREGEAEKQKQYTALVWISRPVSDDDLQTISSLKELKVAQKTPIRVLHRRSPLEREKII 496

Query: 1580 HWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAEILQLDVTDV 1759
            HWM++ERI+GSS Y LLHLCTQAGTYIKEFVHGD GRT PSVGSILGCRAEILQLDVTDV
Sbjct: 497  HWMKVERISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGCRAEILQLDVTDV 556

Query: 1760 KMDCFQA 1780
            KMDCFQA
Sbjct: 557  KMDCFQA 563


>ref|XP_009595593.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Nicotiana tomentosiformis]
 ref|XP_018624870.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Nicotiana tomentosiformis]
          Length = 567

 Score =  625 bits (1611), Expect = 0.0
 Identities = 332/548 (60%), Positives = 396/548 (72%), Gaps = 14/548 (2%)
 Frame = +2

Query: 179  KDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLSTPILSAILHEL 358
            ++ L  LRDAV  LP   +KDL S G C RCIFRL  LHE +CS PSLS      IL ++
Sbjct: 21   EEKLRLLRDAVNELPVNAVKDLWSIGACARCIFRLFGLHELMCSHPSLSISKWYDILADV 80

Query: 359  TEHEG-DGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKGMLMKKHRAS 535
               E  D A+    + +  I   H Q+  +E  FC++CLGILQF++NDD G L+KK  A 
Sbjct: 81   MRKEDVDTADHPQKDGEFSIKQLH-QEPLKETNFCKICLGILQFLYNDDSGTLLKKDSAD 139

Query: 536  DFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWFREKYPSESI 715
            DFA A+ + VKQ  H  DSFSLE SLP  +  N+QAVW Y+K +Y  + WF+EK   + I
Sbjct: 140  DFALAVSEPVKQQHHQIDSFSLEFSLPSALTENDQAVWLYMKNRYHHELWFQEKAEYQFI 199

Query: 716  AVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGCYKRRKVDDI 895
              KD LKL VTNPL+ LL VKS++ S RIRLTY+ SDA   +  I E NG  KRRK    
Sbjct: 200  CTKDILKLLVTNPLQSLLGVKSSQGSLRIRLTYSHSDALT-RDDIQERNGGNKRRKTGKA 258

Query: 896  DFIKNSAGLHK-VERHEPSRNSSIESGDDGT------------GFLAFPIEKVIQCCSLN 1036
             F  +     + V  H  S ++ I  GD  T             +L   + KV + C L 
Sbjct: 259  GFTGDLKTTEESVTPHVESFDNHIGDGDGLTKVCPKDSQDLSCSYLMAQLNKVSKPCCLT 318

Query: 1037 FQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPICQGDSYKFHAA 1216
                ++PI++GGRYLKYSRNVSQT W+IDDERMGEAS+EEIVGG ILPI QGD+YKFHAA
Sbjct: 319  IHCCRSPIFIGGRYLKYSRNVSQTPWVIDDERMGEASVEEIVGGAILPIFQGDNYKFHAA 378

Query: 1217 GREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVKNLKFLGSQGW 1396
            GREDIDVRMLGTGRPFLVEIQNAR+ PS+ LIK+IE KIN+LES+ VRVKNL+ +GSQGW
Sbjct: 379  GREDIDVRMLGTGRPFLVEIQNARKVPSEVLIKEIERKINSLESQLVRVKNLRAVGSQGW 438

Query: 1397 ELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLHRRSPLEREKT 1576
            +LMREGEAEKQKQY ALVWISRP+++DD+QT+SS K++++ QKTPIRVLHRRSPLEREK 
Sbjct: 439  DLMREGEAEKQKQYTALVWISRPVSDDDLQTISSLKELKVAQKTPIRVLHRRSPLEREKI 498

Query: 1577 IHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAEILQLDVTD 1756
            IHWM++ERI+GSS Y LLHLCTQAGTYIKEFVHGD GRT PSVGSILGCRAEILQLDVTD
Sbjct: 499  IHWMKVERISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGCRAEILQLDVTD 558

Query: 1757 VKMDCFQA 1780
            VKMDCFQA
Sbjct: 559  VKMDCFQA 566


>ref|XP_006342101.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Solanum tuberosum]
          Length = 560

 Score =  620 bits (1600), Expect = 0.0
 Identities = 328/558 (58%), Positives = 398/558 (71%), Gaps = 16/558 (2%)
 Frame = +2

Query: 152  HVSSATEANKDD-LGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLST 328
            H+SSA  A +++ L  LR+AV  LP   +KDL S G C RCIFRL  LHE +CS PSLS 
Sbjct: 15   HLSSAEAAAEEEKLRLLREAVHALPVNAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSI 74

Query: 329  PILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKG 508
                 IL ++   +    N  +            Q+   E  FC++CLGILQF++ DD G
Sbjct: 75   SKWCDILEDVMRKKDGEFNEQSH-----------QERSRETNFCKICLGILQFLYYDDTG 123

Query: 509  MLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWF 688
             L+KK+ A DFA  I + VKQ     DSFSLEVSLP  V  N+QAVW Y+K K+  + WF
Sbjct: 124  TLLKKYSADDFAVVISELVKQQYQQIDSFSLEVSLPSSVTENDQAVWLYMKNKFRHELWF 183

Query: 689  REKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGC 868
            +EK   + I+ KD LKL V NPL++LL VKS++SSFR+RLTY+ SDA   +  + + NG 
Sbjct: 184  QEKAECQFISTKDILKLLVINPLQRLLGVKSSQSSFRVRLTYSHSDALT-RDDVQDGNGG 242

Query: 869  YKRRKVDDIDF---IKNSAGLHKVERHEPSRNSSIESGDDGTGF------------LAFP 1003
             KRRK D   F   +K +     V  H  S ++ I  GD  T              L   
Sbjct: 243  NKRRKTDKAGFTGDLKTTG--ESVTPHVESSDNHIVDGDGLTKVCPKESQDLSCNNLMAQ 300

Query: 1004 IEKVIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPI 1183
            ++KV + C L     ++  Y+GGRYLKYSRNVSQTRW+IDDERMGEAS+EEI+GG ILPI
Sbjct: 301  LDKVGKPCCLTIHCCRSSTYIGGRYLKYSRNVSQTRWVIDDERMGEASVEEIIGGAILPI 360

Query: 1184 CQGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRV 1363
             QGD+YKFHAAGREDIDVRMLGTGRPFLVEIQNARQ PS+ LIK+IE KIN+LES +VRV
Sbjct: 361  FQGDNYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEALIKEIERKINSLESHYVRV 420

Query: 1364 KNLKFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVL 1543
            KNL+ +G QGW+LMREGEAEKQKQYAALVWISRP++++D+QT+SS K++++ QKTPIRVL
Sbjct: 421  KNLRAVGIQGWDLMREGEAEKQKQYAALVWISRPVSDEDLQTISSLKELKVAQKTPIRVL 480

Query: 1544 HRRSPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGC 1723
            HRRSPLEREK IHWM+ ER++GSS YFLLHLCTQAGTYIKEFVHGD GRT PS+GSILGC
Sbjct: 481  HRRSPLEREKIIHWMQAERVSGSSQYFLLHLCTQAGTYIKEFVHGDFGRTQPSIGSILGC 540

Query: 1724 RAEILQLDVTDVKMDCFQ 1777
            R EILQLDVTDVKMDCFQ
Sbjct: 541  RTEILQLDVTDVKMDCFQ 558


>ref|XP_009775534.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Nicotiana sylvestris]
 ref|XP_016457703.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 [Nicotiana
            tabacum]
          Length = 567

 Score =  619 bits (1597), Expect = 0.0
 Identities = 321/549 (58%), Positives = 396/549 (72%), Gaps = 13/549 (2%)
 Frame = +2

Query: 173  ANKDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLSTPILSAILH 352
            + +++L  LR+AV  LP   +KDL S G C RC+FRLL L E +CS PSLS      IL 
Sbjct: 19   SEEENLRLLREAVNELPVNAVKDLWSIGACVRCVFRLLGLRELMCSHPSLSISKWCDILE 78

Query: 353  ELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKGMLMKKHRA 532
            ++   E         +  +L + +  Q+  +E  FC++CLGILQF++NDD G L+KK  A
Sbjct: 79   DVMRKEDGDTADQPQKDGELSNKQLHQEPLKETNFCKICLGILQFLYNDDNGTLLKKDSA 138

Query: 533  SDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWFREKYPSES 712
            +DFA  I + +KQ  H  DSFSLEVS+P  +   +QAV  Y+K +Y  + WF+EK   + 
Sbjct: 139  NDFALVIAELLKQQHHQIDSFSLEVSVPSALAETDQAVRLYMKNRYHHELWFQEKAECQF 198

Query: 713  IAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGCYKRRKVDD 892
            I+ KD LKL V NPL+KLL VKS++SSFR+RLTY+ SDA   +  + E  G  KRRK D 
Sbjct: 199  ISTKDILKLLVINPLQKLLGVKSSQSSFRVRLTYSHSDALT-RDDVQERAGGNKRRKTDK 257

Query: 893  IDFIKN-SAGLHKVERHEPSRNSSIESGDD------------GTGFLAFPIEKVIQCCSL 1033
              F  +       V  H  S ++ I  GD                +L   + KV + C L
Sbjct: 258  AGFTGDLKTTKESVTPHVESFDNHIGDGDGLMKLCPKDSQDLSCSYLMAQLNKVSKPCCL 317

Query: 1034 NFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPICQGDSYKFHA 1213
                 ++PI++GGRYLKYSRNVSQTRW+IDDERMGEAS+EEIVGG ILPI QGD+YKFHA
Sbjct: 318  TIHCCRSPIFIGGRYLKYSRNVSQTRWVIDDERMGEASVEEIVGGAILPIFQGDNYKFHA 377

Query: 1214 AGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVKNLKFLGSQG 1393
            AGREDIDVRMLGTGRPFLVEIQNARQ PS+ LIK+IE KIN++ES+ VRVKNL+ +GSQG
Sbjct: 378  AGREDIDVRMLGTGRPFLVEIQNARQVPSEVLIKEIERKINSVESRLVRVKNLRAVGSQG 437

Query: 1394 WELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLHRRSPLEREK 1573
            W+LMREGEAEKQKQY ALVWISRP++++D+QT+SS K++++ Q+TPIRVLHRRSPLEREK
Sbjct: 438  WDLMREGEAEKQKQYTALVWISRPVSDEDLQTISSLKELKVAQRTPIRVLHRRSPLEREK 497

Query: 1574 TIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAEILQLDVT 1753
             IHWM++ERI+GSS Y LLHLCTQAGTYIKEFVHGD GRT PSVGSILGCR EILQLDVT
Sbjct: 498  IIHWMKVERISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGCRTEILQLDVT 557

Query: 1754 DVKMDCFQA 1780
            DVKMDCFQA
Sbjct: 558  DVKMDCFQA 566


>ref|XP_015161917.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Solanum tuberosum]
          Length = 557

 Score =  619 bits (1596), Expect = 0.0
 Identities = 326/555 (58%), Positives = 396/555 (71%), Gaps = 13/555 (2%)
 Frame = +2

Query: 152  HVSSATEANKDD-LGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLST 328
            H+SSA  A +++ L  LR+AV  LP   +KDL S G C RCIFRL  LHE +CS PSLS 
Sbjct: 15   HLSSAEAAAEEEKLRLLREAVHALPVNAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSI 74

Query: 329  PILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKG 508
                 IL ++   +    N  +            Q+   E  FC++CLGILQF++ DD G
Sbjct: 75   SKWCDILEDVMRKKDGEFNEQSH-----------QERSRETNFCKICLGILQFLYYDDTG 123

Query: 509  MLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWF 688
             L+KK+ A DFA  I + VKQ     DSFSLEVSLP  V  N+QAVW Y+K K+  + WF
Sbjct: 124  TLLKKYSADDFAVVISELVKQQYQQIDSFSLEVSLPSSVTENDQAVWLYMKNKFRHELWF 183

Query: 689  REKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGC 868
            +EK   + I+ KD LKL V NPL++LL VKS++SSFR+RLTY+ SDA   +  + + NG 
Sbjct: 184  QEKAECQFISTKDILKLLVINPLQRLLGVKSSQSSFRVRLTYSHSDALT-RDDVQDGNGG 242

Query: 869  YKRRKVDDIDFIKNSAGLHKVERHEPSRNSSIESGDDGTGF------------LAFPIEK 1012
             KRRK      +K +     V  H  S ++ I  GD  T              L   ++K
Sbjct: 243  NKRRKTGFTGDLKTTG--ESVTPHVESSDNHIVDGDGLTKVCPKESQDLSCNNLMAQLDK 300

Query: 1013 VIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPICQG 1192
            V + C L     ++  Y+GGRYLKYSRNVSQTRW+IDDERMGEAS+EEI+GG ILPI QG
Sbjct: 301  VGKPCCLTIHCCRSSTYIGGRYLKYSRNVSQTRWVIDDERMGEASVEEIIGGAILPIFQG 360

Query: 1193 DSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVKNL 1372
            D+YKFHAAGREDIDVRMLGTGRPFLVEIQNARQ PS+ LIK+IE KIN+LES +VRVKNL
Sbjct: 361  DNYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEALIKEIERKINSLESHYVRVKNL 420

Query: 1373 KFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLHRR 1552
            + +G QGW+LMREGEAEKQKQYAALVWISRP++++D+QT+SS K++++ QKTPIRVLHRR
Sbjct: 421  RAVGIQGWDLMREGEAEKQKQYAALVWISRPVSDEDLQTISSLKELKVAQKTPIRVLHRR 480

Query: 1553 SPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAE 1732
            SPLEREK IHWM+ ER++GSS YFLLHLCTQAGTYIKEFVHGD GRT PS+GSILGCR E
Sbjct: 481  SPLEREKIIHWMQAERVSGSSQYFLLHLCTQAGTYIKEFVHGDFGRTQPSIGSILGCRTE 540

Query: 1733 ILQLDVTDVKMDCFQ 1777
            ILQLDVTDVKMDCFQ
Sbjct: 541  ILQLDVTDVKMDCFQ 555


>ref|XP_021641351.1| putative tRNA pseudouridine synthase Pus10 [Hevea brasiliensis]
          Length = 563

 Score =  619 bits (1596), Expect = 0.0
 Identities = 326/559 (58%), Positives = 404/559 (72%), Gaps = 12/559 (2%)
 Frame = +2

Query: 134  NDDEEAHVSSATEANKDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSR 313
            ++ E A  +S T    +D+   +DAVR+LP  ++KDLLS GVC RCIFRL  +H  + S 
Sbjct: 3    SESEAADFNSHTAVGGEDMQLFKDAVRSLPSHVVKDLLSIGVCMRCIFRLFGMHGHVYSC 62

Query: 314  PSLSTPILSAILHELTEHEGDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVH 493
             S S+ ++ AIL E +  E   A SS    +D  DT    D + E   C +CLGILQF++
Sbjct: 63   SSPSSLVMCAILGEPSCPEEHMAVSSLKGDEDKKDTSSSDDSEVEPEICSICLGILQFIY 122

Query: 494  NDDKGMLMKKHRASDFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYG 673
             D KGML+KK  A   A +I + +K++G+  DSFSLEVS+PP++  NE  +  Y+K+KY 
Sbjct: 123  YDAKGMLVKKRSAISLALSISEQIKKEGNQLDSFSLEVSIPPVIQENEHNIHLYMKRKYR 182

Query: 674  SQPWFREKYPSESIAVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIH 853
            S+ WF E+  S+ I+ K+ LK ++ N LEK+LDVKS  SS RIRLTY  +  S+M   + 
Sbjct: 183  SELWFEERL-SKCISTKEALKFAMANLLEKVLDVKSGPSSCRIRLTYAQTKPSSMVPNLA 241

Query: 854  ESN-GCYKRRKVDD--IDFIKNS---AGLHKVERHEPSRNSSIESGDDG------TGFLA 997
                GC +R+ V +   D + N    A     +    +RN+  ES  +G      +  + 
Sbjct: 242  AGGEGCKRRKTVANPGFDSVNNKLVDASNECFDFSTTNRNALGESSTNGLEDHESSECII 301

Query: 998  FPIEKVIQCCSLNFQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNIL 1177
            FP +KV + C L F +Y+TPIY+GGRYLK+SRNVSQTRWIIDDERMGEAS+EEI+GGNIL
Sbjct: 302  FPKDKVNEPCHLVFLYYRTPIYIGGRYLKFSRNVSQTRWIIDDERMGEASVEEIIGGNIL 361

Query: 1178 PICQGDSYKFHAAGREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFV 1357
            P C GDSYKFHAAGREDIDVRMLG+GRPFLVEIQNARQ PS+ L+K+IE KINNL +K V
Sbjct: 362  PACHGDSYKFHAAGREDIDVRMLGSGRPFLVEIQNARQVPSEALVKEIETKINNLNTKLV 421

Query: 1358 RVKNLKFLGSQGWELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIR 1537
             VKNLK + ++GW LM EGEAEKQKQY ALVWISRPL + D+Q+++S KD+QILQKTPIR
Sbjct: 422  AVKNLKMVANEGWTLMHEGEAEKQKQYCALVWISRPLEDQDLQSIASLKDLQILQKTPIR 481

Query: 1538 VLHRRSPLEREKTIHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSIL 1717
            VLHRRSPLEREK IHWM+IE IA SS YFLLHLCTQAGTYIKEFVHGDLGRT PS+GSIL
Sbjct: 482  VLHRRSPLEREKIIHWMKIETIAESSQYFLLHLCTQAGTYIKEFVHGDLGRTCPSIGSIL 541

Query: 1718 GCRAEILQLDVTDVKMDCF 1774
            GCRAEILQLDVTDVKMDCF
Sbjct: 542  GCRAEILQLDVTDVKMDCF 560


>ref|XP_016432383.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Nicotiana tabacum]
          Length = 564

 Score =  619 bits (1596), Expect = 0.0
 Identities = 330/547 (60%), Positives = 394/547 (72%), Gaps = 13/547 (2%)
 Frame = +2

Query: 179  KDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLSTPILSAILHEL 358
            ++ L  LRDAV  LP   +KDL S G C RCIFRL  LHE +CS PSLS      IL ++
Sbjct: 21   EETLRLLRDAVNELPLNAVKDLWSIGACARCIFRLFGLHELMCSHPSLSISKWYDILADV 80

Query: 359  TEHE-GDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKGMLMKKHRAS 535
               E  D A+    + +  I   H Q+  +E  FC++CLGILQF++NDD G L+KK  A 
Sbjct: 81   MRKEDADTADHPQKDGEFSIKQLH-QEPLKETNFCKICLGILQFLYNDDSGTLLKKDSAD 139

Query: 536  DFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWFREKYPSESI 715
            DFA A+ + VKQ  H  DSFSLE SLP  +  N+QAVW Y+K +Y  + WF+EK   + I
Sbjct: 140  DFALAVSEPVKQQHHQIDSFSLEFSLPSALTENDQAVWLYMKNRYRHELWFQEKAEYQFI 199

Query: 716  AVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGCYKRRKVDDI 895
              KD LKL VTNPL+ LL VKS++ S RIRLTY+ SDA   +  I E NG  KRRK    
Sbjct: 200  CTKDILKLLVTNPLQSLLGVKSSQGSLRIRLTYSHSDALT-RDDIQERNGGNKRRKTGFT 258

Query: 896  DFIKNSAGLHKVERHEPSRNSSIESGDDGT------------GFLAFPIEKVIQCCSLNF 1039
              +K +     V  H  S ++ I  GD  T             +L   + KV + C L  
Sbjct: 259  GDLKTTE--ESVTPHVESFDNHIGDGDGLTKVCPKDSQDLSCSYLMAQLNKVSKPCCLTI 316

Query: 1040 QFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPICQGDSYKFHAAG 1219
               ++PI++GGRY KYSRNVSQTRW+IDDERMGEAS+EEIVG  ILPI QGD+YKFHAAG
Sbjct: 317  HCCRSPIFIGGRYFKYSRNVSQTRWVIDDERMGEASVEEIVGRAILPIFQGDNYKFHAAG 376

Query: 1220 REDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVKNLKFLGSQGWE 1399
            REDIDVRMLGTGRPFLVEIQNAR+ PS+ LIK+IE KIN+LES+ VRVKNL+ +GSQGW+
Sbjct: 377  REDIDVRMLGTGRPFLVEIQNARKVPSEVLIKEIERKINSLESQLVRVKNLRAVGSQGWD 436

Query: 1400 LMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLHRRSPLEREKTI 1579
            LMREGEAEKQKQY ALVWISRP+++DD+QT+SS K++++ QKTPIRVLHRRSPLEREK I
Sbjct: 437  LMREGEAEKQKQYTALVWISRPVSDDDLQTISSLKELKVAQKTPIRVLHRRSPLEREKII 496

Query: 1580 HWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAEILQLDVTDV 1759
            HWM++ERI+GSS Y LLHLCTQAGTYIKEFVHGD GRT PSVGSILG RAEILQLDVTDV
Sbjct: 497  HWMKVERISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGRRAEILQLDVTDV 556

Query: 1760 KMDCFQA 1780
            KMDCFQA
Sbjct: 557  KMDCFQA 563


>ref|XP_016432382.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Nicotiana tabacum]
          Length = 567

 Score =  618 bits (1594), Expect = 0.0
 Identities = 330/548 (60%), Positives = 394/548 (71%), Gaps = 14/548 (2%)
 Frame = +2

Query: 179  KDDLGFLRDAVRTLPPQILKDLLSNGVCTRCIFRLLILHEEICSRPSLSTPILSAILHEL 358
            ++ L  LRDAV  LP   +KDL S G C RCIFRL  LHE +CS PSLS      IL ++
Sbjct: 21   EETLRLLRDAVNELPLNAVKDLWSIGACARCIFRLFGLHELMCSHPSLSISKWYDILADV 80

Query: 359  TEHE-GDGANSSAGETKDLIDTEHVQDFQEEFRFCRVCLGILQFVHNDDKGMLMKKHRAS 535
               E  D A+    + +  I   H Q+  +E  FC++CLGILQF++NDD G L+KK  A 
Sbjct: 81   MRKEDADTADHPQKDGEFSIKQLH-QEPLKETNFCKICLGILQFLYNDDSGTLLKKDSAD 139

Query: 536  DFAAAIGDAVKQDGHNFDSFSLEVSLPPIVDVNEQAVWSYIKKKYGSQPWFREKYPSESI 715
            DFA A+ + VKQ  H  DSFSLE SLP  +  N+QAVW Y+K +Y  + WF+EK   + I
Sbjct: 140  DFALAVSEPVKQQHHQIDSFSLEFSLPSALTENDQAVWLYMKNRYRHELWFQEKAEYQFI 199

Query: 716  AVKDFLKLSVTNPLEKLLDVKSNESSFRIRLTYTLSDASAMKQPIHESNGCYKRRKVDDI 895
              KD LKL VTNPL+ LL VKS++ S RIRLTY+ SDA   +  I E NG  KRRK    
Sbjct: 200  CTKDILKLLVTNPLQSLLGVKSSQGSLRIRLTYSHSDALT-RDDIQERNGGNKRRKTGKA 258

Query: 896  DFIKNSAGLHK-VERHEPSRNSSIESGDDGT------------GFLAFPIEKVIQCCSLN 1036
             F  +     + V  H  S ++ I  GD  T             +L   + KV + C L 
Sbjct: 259  GFTGDLKTTEESVTPHVESFDNHIGDGDGLTKVCPKDSQDLSCSYLMAQLNKVSKPCCLT 318

Query: 1037 FQFYKTPIYVGGRYLKYSRNVSQTRWIIDDERMGEASIEEIVGGNILPICQGDSYKFHAA 1216
                ++PI++GGRY KYSRNVSQTRW+IDDERMGEAS+EEIVG  ILPI QGD+YKFHAA
Sbjct: 319  IHCCRSPIFIGGRYFKYSRNVSQTRWVIDDERMGEASVEEIVGRAILPIFQGDNYKFHAA 378

Query: 1217 GREDIDVRMLGTGRPFLVEIQNARQFPSDELIKDIEMKINNLESKFVRVKNLKFLGSQGW 1396
            GREDIDVRMLGTGRPFLVEIQNAR+ PS+ LIK+IE KIN+LES+ VRVKNL+ +GSQGW
Sbjct: 379  GREDIDVRMLGTGRPFLVEIQNARKVPSEVLIKEIERKINSLESQLVRVKNLRAVGSQGW 438

Query: 1397 ELMREGEAEKQKQYAALVWISRPLNNDDVQTLSSPKDMQILQKTPIRVLHRRSPLEREKT 1576
            +LMREGEAEKQKQY ALVWISRP+++DD+QT+SS K++++ QKTPIRVLHRRSPLEREK 
Sbjct: 439  DLMREGEAEKQKQYTALVWISRPVSDDDLQTISSLKELKVAQKTPIRVLHRRSPLEREKI 498

Query: 1577 IHWMRIERIAGSSHYFLLHLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAEILQLDVTD 1756
            IHWM++ERI+GSS Y LLHLCTQAGTYIKEFVHGD GRT PSVGSILG RAEILQLDVTD
Sbjct: 499  IHWMKVERISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGRRAEILQLDVTD 558

Query: 1757 VKMDCFQA 1780
            VKMDCFQA
Sbjct: 559  VKMDCFQA 566