BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia30_contig00028235
         (461 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011095137.1| uncharacterized protein LOC105174662 [Sesamu...   211   1e-67
gb|PIN21378.1| hypothetical protein CDL12_05923 [Handroanthus im...   186   1e-57
gb|KZV40866.1| hypothetical protein F511_22805 [Dorcoceras hygro...    86   8e-19
gb|OVA17281.1| hypothetical protein BVC80_1837g79 [Macleaya cord...    70   2e-12
ref|XP_021687866.1| uncharacterized protein LOC110670200 [Hevea ...    69   4e-11
ref|XP_007033537.2| PREDICTED: uncharacterized protein LOC186022...    67   3e-10
ref|XP_022760946.1| uncharacterized protein LOC111307197 isoform...    65   5e-10
gb|EOY04463.1| Uncharacterized protein TCM_019705 [Theobroma cacao]    66   6e-10
ref|XP_022760945.1| uncharacterized protein LOC111307197 isoform...    65   8e-10
ref|XP_022760944.1| uncharacterized protein LOC111307197 isoform...    65   9e-10
ref|XP_021619469.1| uncharacterized protein LOC110620161 [Maniho...    64   2e-09
ref|XP_006374050.1| hypothetical protein POPTR_0016s14330g [Popu...    62   7e-09
gb|PNS99443.1| hypothetical protein POPTR_016G136300v3 [Populus ...    62   1e-08
ref|XP_011002604.1| PREDICTED: uncharacterized protein LOC105109...    62   2e-08
ref|XP_018628563.1| PREDICTED: uncharacterized protein LOC104102...    61   2e-08
ref|XP_016467142.1| PREDICTED: uncharacterized protein LOC107789...    61   3e-08
ref|XP_009608990.1| PREDICTED: uncharacterized protein LOC104102...    61   3e-08
ref|XP_010653342.1| PREDICTED: uncharacterized protein LOC104880...    60   9e-08
ref|XP_019238226.1| PREDICTED: uncharacterized protein LOC109218...    59   1e-07
ref|XP_019238225.1| PREDICTED: uncharacterized protein LOC109218...    59   2e-07

>ref|XP_011095137.1| uncharacterized protein LOC105174662 [Sesamum indicum]
          Length = 168

 Score =  211 bits (538), Expect = 1e-67
 Identities = 105/156 (67%), Positives = 120/156 (76%), Gaps = 4/156 (2%)
 Frame = -1

Query: 458 MSHVDQQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSKPKSSF 279
           M+   QQQPWTLSLRAKAKNLDFK K SR+LPV KLFRFS+QF++HPY L+IKSKP+SSF
Sbjct: 1   MTQAYQQQPWTLSLRAKAKNLDFKLKASRMLPVCKLFRFSIQFKLHPYFLEIKSKPESSF 60

Query: 278 SLNPKSLKLKFLRIFKNFHVKRSKNKGIARSWFPHKNYFNGRRKRCIWLWVLLFVMVGCF 99
           SL PK LKL FL IFK F  KR++NKGI RSW  HKNY  GRRK  IWLWVLL  +VGCF
Sbjct: 61  SLKPKCLKLIFLEIFKKFQNKRARNKGIVRSWLAHKNYLYGRRKTWIWLWVLLSAVVGCF 120

Query: 98  IIMRLSSFL----IWKCVWRAWNNAAPSGFYYQFFK 3
           I+M+LS +L    +WK   +A N AAPSGF YQ  K
Sbjct: 121 IVMQLSYYLGEGFLWKYSCKALNYAAPSGFLYQLSK 156


>gb|PIN21378.1| hypothetical protein CDL12_05923 [Handroanthus impetiginosus]
          Length = 163

 Score =  186 bits (472), Expect = 1e-57
 Identities = 89/152 (58%), Positives = 115/152 (75%)
 Frame = -1

Query: 458 MSHVDQQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSKPKSSF 279
           M+H++QQ+PWTLS RAK+KNLDFKF  S+ILPV KL RFS+Q ++H Y  +IKS+P SSF
Sbjct: 1   MTHLEQQEPWTLSFRAKSKNLDFKFSASKILPVCKLSRFSIQLKLHKYLFEIKSQPDSSF 60

Query: 278 SLNPKSLKLKFLRIFKNFHVKRSKNKGIARSWFPHKNYFNGRRKRCIWLWVLLFVMVGCF 99
           S  P SLK KFL+IF+ F  KR KN+GI RSW+  KNYF  RR+  + L+VLLF MV CF
Sbjct: 61  SFKPNSLKQKFLQIFRIFQRKRIKNRGIVRSWY-RKNYFYERRRAYLLLYVLLFAMVSCF 119

Query: 98  IIMRLSSFLIWKCVWRAWNNAAPSGFYYQFFK 3
           +I +L ++ +WK + +A N AAPSG  YQFF+
Sbjct: 120 LIRQLRTYFVWKYLCKALNYAAPSGLLYQFFQ 151


>gb|KZV40866.1| hypothetical protein F511_22805 [Dorcoceras hygrometricum]
          Length = 122

 Score = 86.3 bits (212), Expect = 8e-19
 Identities = 41/92 (44%), Positives = 62/92 (67%)
 Frame = -1

Query: 458 MSHVDQQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSKPKSSF 279
           M  V+Q+QPW++++RAK  N   + K+S++ P+ K+ RFSV+F++HP+  +IKS+P SSF
Sbjct: 1   MLQVNQKQPWSITVRAKPSNFYLRLKMSKLSPLCKISRFSVRFKLHPFHFRIKSEPHSSF 60

Query: 278 SLNPKSLKLKFLRIFKNFHVKRSKNKGIARSW 183
           S  PKSLK    RIF+ F   R+    I  +W
Sbjct: 61  SWKPKSLK---RRIFQIFRPNRTSRDDIVGTW 89


>gb|OVA17281.1| hypothetical protein BVC80_1837g79 [Macleaya cordata]
          Length = 135

 Score = 70.1 bits (170), Expect = 2e-12
 Identities = 39/102 (38%), Positives = 60/102 (58%), Gaps = 5/102 (4%)
 Frame = -1

Query: 458 MSHVDQQQPWTLSLRAKAKNLDFKFKLSRILPVSKLF--RFSVQFRVHPYSLQIKSKPKS 285
           M  + Q QPW +S+RAKAKN DFKF  S+ILP    +  RFSV  ++  + L++KS+   
Sbjct: 1   MEAIKQDQPWRISVRAKAKNFDFKFSASKILPSWGFYGTRFSVLIKLQRFFLKVKSETGD 60

Query: 284 SFSLNPKS---LKLKFLRIFKNFHVKRSKNKGIARSWFPHKN 168
           S     +S    K KFL++ + F +KR +++ ++ S    KN
Sbjct: 61  SAQRRRRSSGTFKSKFLQLLEKFRLKRVRDEKVSTSVIKKKN 102


>ref|XP_021687866.1| uncharacterized protein LOC110670200 [Hevea brasiliensis]
          Length = 224

 Score = 68.6 bits (166), Expect = 4e-11
 Identities = 33/84 (39%), Positives = 53/84 (63%)
 Frame = -1

Query: 443 QQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSKPKSSFSLNPK 264
           +Q PW +++ A+A    FKFK ++I P SK F+FS+  R+H + LQ+K+   S+    P 
Sbjct: 7   KQLPWKININARASKFHFKFKATKIDPTSKFFKFSLFLRLHAFLLQVKT--DSTPRQPPT 64

Query: 263 SLKLKFLRIFKNFHVKRSKNKGIA 192
           +LK KFL+ F+ F+   +K + IA
Sbjct: 65  TLKSKFLKFFRKFNTSSAKKRAIA 88


>ref|XP_007033537.2| PREDICTED: uncharacterized protein LOC18602222 [Theobroma cacao]
          Length = 238

 Score = 66.6 bits (161), Expect = 3e-10
 Identities = 30/82 (36%), Positives = 56/82 (68%), Gaps = 3/82 (3%)
 Frame = -1

Query: 443 QQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSKPKSSFSL--- 273
           QQQ W +S++A+A+N++FK + ++ILP  K  RFS+  R+H + L+++ K +S+ S+   
Sbjct: 6   QQQSWKVSIQARAQNVNFKLQATKILPTWKFHRFSLLLRLHKFVLKLQVKSRSTLSISIQ 65

Query: 272 NPKSLKLKFLRIFKNFHVKRSK 207
           N ++LK  FL++ +   ++R K
Sbjct: 66  NKRTLKSTFLKLLEKLRIRRPK 87


>ref|XP_022760946.1| uncharacterized protein LOC111307197 isoform X3 [Durio zibethinus]
          Length = 185

 Score = 65.1 bits (157), Expect = 5e-10
 Identities = 31/87 (35%), Positives = 56/87 (64%), Gaps = 3/87 (3%)
 Frame = -1

Query: 458 MSHVDQQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSKPKSSF 279
           M   +QQQ W ++++A+A+N  FK K ++ILP  K  RFS+  R+H   L+++ +P S+ 
Sbjct: 1   MQAEEQQQSWKVNIQARAQNFSFKLKATKILPTGKFQRFSLLLRLHKVILKLQLEPGSTV 60

Query: 278 SLN---PKSLKLKFLRIFKNFHVKRSK 207
           S++    ++LK  FLR+ +   ++R+K
Sbjct: 61  SISTQQKRTLKSTFLRLLEKLRLRRAK 87


>gb|EOY04463.1| Uncharacterized protein TCM_019705 [Theobroma cacao]
          Length = 300

 Score = 66.2 bits (160), Expect = 6e-10
 Identities = 30/82 (36%), Positives = 55/82 (67%), Gaps = 3/82 (3%)
 Frame = -1

Query: 443 QQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSKPKSSFSL--- 273
           QQQ W +S++A+A+N +FK + ++ILP  K  RFS+  R+H + L+++ K +S+ S+   
Sbjct: 68  QQQSWKVSIQARAQNFNFKLQATKILPTWKFHRFSLLLRLHKFVLKLQVKSRSTLSISIQ 127

Query: 272 NPKSLKLKFLRIFKNFHVKRSK 207
           N ++LK  FL++ +   ++R K
Sbjct: 128 NKRTLKSTFLKLLEKLRIRRPK 149


>ref|XP_022760945.1| uncharacterized protein LOC111307197 isoform X2 [Durio zibethinus]
          Length = 223

 Score = 65.1 bits (157), Expect = 8e-10
 Identities = 31/87 (35%), Positives = 56/87 (64%), Gaps = 3/87 (3%)
 Frame = -1

Query: 458 MSHVDQQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSKPKSSF 279
           M   +QQQ W ++++A+A+N  FK K ++ILP  K  RFS+  R+H   L+++ +P S+ 
Sbjct: 1   MQAEEQQQSWKVNIQARAQNFSFKLKATKILPTGKFQRFSLLLRLHKVILKLQLEPGSTV 60

Query: 278 SLN---PKSLKLKFLRIFKNFHVKRSK 207
           S++    ++LK  FLR+ +   ++R+K
Sbjct: 61  SISTQQKRTLKSTFLRLLEKLRLRRAK 87


>ref|XP_022760944.1| uncharacterized protein LOC111307197 isoform X1 [Durio zibethinus]
          Length = 232

 Score = 65.1 bits (157), Expect = 9e-10
 Identities = 31/87 (35%), Positives = 56/87 (64%), Gaps = 3/87 (3%)
 Frame = -1

Query: 458 MSHVDQQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSKPKSSF 279
           M   +QQQ W ++++A+A+N  FK K ++ILP  K  RFS+  R+H   L+++ +P S+ 
Sbjct: 1   MQAEEQQQSWKVNIQARAQNFSFKLKATKILPTGKFQRFSLLLRLHKVILKLQLEPGSTV 60

Query: 278 SLN---PKSLKLKFLRIFKNFHVKRSK 207
           S++    ++LK  FLR+ +   ++R+K
Sbjct: 61  SISTQQKRTLKSTFLRLLEKLRLRRAK 87


>ref|XP_021619469.1| uncharacterized protein LOC110620161 [Manihot esculenta]
 gb|OAY44684.1| hypothetical protein MANES_08G171400 [Manihot esculenta]
          Length = 233

 Score = 64.3 bits (155), Expect = 2e-09
 Identities = 32/82 (39%), Positives = 50/82 (60%)
 Frame = -1

Query: 440 QQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSKPKSSFSLNPKS 261
           QQ W +S+ A+AK   FKFK + I P  K F+ S+  R+H + LQ+K+   S+    P  
Sbjct: 6   QQSWKISINARAKKFHFKFKATTIDPTWKFFKLSLFLRLHEFLLQVKT--DSTPRQPPTP 63

Query: 260 LKLKFLRIFKNFHVKRSKNKGI 195
           LK KFL+IF+ F+ + +K + +
Sbjct: 64  LKSKFLKIFRRFNARSAKKRAV 85


>ref|XP_006374050.1| hypothetical protein POPTR_0016s14330g [Populus trichocarpa]
          Length = 170

 Score = 61.6 bits (148), Expect = 7e-09
 Identities = 32/88 (36%), Positives = 53/88 (60%), Gaps = 3/88 (3%)
 Frame = -1

Query: 446 DQQQPWTLSLRAKAKNLDFKFKLSRI-LPVSKLFRFSVQFRVHPYSLQIKSKPKSSFS-- 276
           ++QQ W + + A+AK   FKFK ++   P+ K  +FS+  R+H + LQ+ ++ ++S    
Sbjct: 4   EKQQTWKIKVHARAKKFHFKFKATKTDQPIWKSPKFSIFLRIHRFFLQVNTESRNSIPRW 63

Query: 275 LNPKSLKLKFLRIFKNFHVKRSKNKGIA 192
             P++LK KFLR F+ F    SK + IA
Sbjct: 64  KKPRTLKSKFLRFFQKFKTLPSKKQAIA 91


>gb|PNS99443.1| hypothetical protein POPTR_016G136300v3 [Populus trichocarpa]
          Length = 215

 Score = 61.6 bits (148), Expect = 1e-08
 Identities = 32/88 (36%), Positives = 53/88 (60%), Gaps = 3/88 (3%)
 Frame = -1

Query: 446 DQQQPWTLSLRAKAKNLDFKFKLSRI-LPVSKLFRFSVQFRVHPYSLQIKSKPKSSFS-- 276
           ++QQ W + + A+AK   FKFK ++   P+ K  +FS+  R+H + LQ+ ++ ++S    
Sbjct: 4   EKQQTWKIKVHARAKKFHFKFKATKTDQPIWKSPKFSIFLRIHRFFLQVNTESRNSIPRW 63

Query: 275 LNPKSLKLKFLRIFKNFHVKRSKNKGIA 192
             P++LK KFLR F+ F    SK + IA
Sbjct: 64  KKPRTLKSKFLRFFQKFKTLPSKKQAIA 91


>ref|XP_011002604.1| PREDICTED: uncharacterized protein LOC105109563 [Populus
           euphratica]
 ref|XP_011002605.1| PREDICTED: uncharacterized protein LOC105109563 [Populus
           euphratica]
          Length = 242

 Score = 61.6 bits (148), Expect = 2e-08
 Identities = 31/88 (35%), Positives = 53/88 (60%), Gaps = 3/88 (3%)
 Frame = -1

Query: 446 DQQQPWTLSLRAKAKNLDFKFKLSRI-LPVSKLFRFSVQFRVHPYSLQIKSKPKSSFS-- 276
           ++QQ W + + A+AK   FKFK ++   P+ K  +FS+  R+H + LQ+ ++ ++S    
Sbjct: 4   EKQQTWKIKVHARAKRFHFKFKATKTDQPIWKSPKFSIFLRIHRFFLQVNTESRNSIPRW 63

Query: 275 LNPKSLKLKFLRIFKNFHVKRSKNKGIA 192
             P++LK KFLR F+ F    SK + +A
Sbjct: 64  KKPRTLKSKFLRFFRKFKTLPSKKQAVA 91


>ref|XP_018628563.1| PREDICTED: uncharacterized protein LOC104102855 isoform X2
           [Nicotiana tomentosiformis]
          Length = 205

 Score = 60.8 bits (146), Expect = 2e-08
 Identities = 38/91 (41%), Positives = 51/91 (56%), Gaps = 2/91 (2%)
 Frame = -1

Query: 443 QQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSK-PKSSFSLNP 267
           Q   W L+++AKAKNLDFK   S  LP  K F FS++F+   + + +KS+ P S      
Sbjct: 6   QCHSWKLNVQAKAKNLDFKLVASNFLPKCKFFPFSLKFKYCRFLIHLKSETPTSPNPQKS 65

Query: 266 KSLKLKFLRIFKNFHV-KRSKNKGIARSWFP 177
           KSLK K  R F+   + +RSKN   A S  P
Sbjct: 66  KSLKSKLHRFFERLRIRRRSKNNSRAISSKP 96


>ref|XP_016467142.1| PREDICTED: uncharacterized protein LOC107789788 [Nicotiana tabacum]
          Length = 213

 Score = 60.8 bits (146), Expect = 3e-08
 Identities = 38/91 (41%), Positives = 51/91 (56%), Gaps = 2/91 (2%)
 Frame = -1

Query: 443 QQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSK-PKSSFSLNP 267
           Q   W L+++AKAKNLDFK   S  LP  K F FS++F+   + + +KS+ P S      
Sbjct: 6   QCHSWKLNVQAKAKNLDFKLVASNFLPKCKFFPFSLKFKYCRFLIHLKSETPTSPNPQKS 65

Query: 266 KSLKLKFLRIFKNFHV-KRSKNKGIARSWFP 177
           KSLK K  R F+   + +RSKN   A S  P
Sbjct: 66  KSLKSKLHRFFERLRIRRRSKNNSRAISSKP 96


>ref|XP_009608990.1| PREDICTED: uncharacterized protein LOC104102855 isoform X1
           [Nicotiana tomentosiformis]
          Length = 213

 Score = 60.8 bits (146), Expect = 3e-08
 Identities = 38/91 (41%), Positives = 51/91 (56%), Gaps = 2/91 (2%)
 Frame = -1

Query: 443 QQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSK-PKSSFSLNP 267
           Q   W L+++AKAKNLDFK   S  LP  K F FS++F+   + + +KS+ P S      
Sbjct: 6   QCHSWKLNVQAKAKNLDFKLVASNFLPKCKFFPFSLKFKYCRFLIHLKSETPTSPNPQKS 65

Query: 266 KSLKLKFLRIFKNFHV-KRSKNKGIARSWFP 177
           KSLK K  R F+   + +RSKN   A S  P
Sbjct: 66  KSLKSKLHRFFERLRIRRRSKNNSRAISSKP 96


>ref|XP_010653342.1| PREDICTED: uncharacterized protein LOC104880011 [Vitis vinifera]
          Length = 236

 Score = 59.7 bits (143), Expect = 9e-08
 Identities = 34/87 (39%), Positives = 51/87 (58%), Gaps = 3/87 (3%)
 Frame = -1

Query: 443 QQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSK---PKSSFSL 273
           Q+QPW+L + AKAKN +FK K ++I+P  K FR SV  +V  +SL+ +S+   P S    
Sbjct: 8   QKQPWSLQVHAKAKNFNFKLKATQIIPSHKFFRLSVFLKVCSFSLKARSERGPPASRQHR 67

Query: 272 NPKSLKLKFLRIFKNFHVKRSKNKGIA 192
            P  L+ KF RI +    + +K   I+
Sbjct: 68  TP--LRSKFARILEKIRSRGTKKPAIS 92


>ref|XP_019238226.1| PREDICTED: uncharacterized protein LOC109218326 isoform X2
           [Nicotiana attenuata]
 gb|OIT21877.1| hypothetical protein A4A49_33507 [Nicotiana attenuata]
          Length = 212

 Score = 58.9 bits (141), Expect = 1e-07
 Identities = 55/191 (28%), Positives = 78/191 (40%), Gaps = 51/191 (26%)
 Frame = -1

Query: 443 QQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSK-PKSSFSLNP 267
           Q   W L+++AKAKNLDFK   S  LP  K F FS++F+   + + +KS+ P        
Sbjct: 6   QCHSWKLNVQAKAKNLDFKLVASNFLPNCKFFPFSLKFKYCRFFIHLKSETPTFPNPQKS 65

Query: 266 KSLKLKFLRIFKNFHV-KRSKNKGIARSWFPHK------------------NYFNGRRKR 144
           KSLK K  R  +   + +RSKN   A S  P                         R K 
Sbjct: 66  KSLKSKLHRFLERLRIRRRSKNNSRAISSKPKNKATISQSSVVVGNLALFIQSLYQRDKD 125

Query: 143 CIWL---------------------WVLLFVMVGCFII------MRLSSFLIWKCV---- 57
            I++                     W+LL  ++G  ++      +R SS+    C+    
Sbjct: 126 GIFILGSTVFICFASIFKSFMARKSWILLVFVIGAIVLCSNVMHLRFSSYFDHHCLSRFF 185

Query: 56  WRAWNNAAPSG 24
           WRA  NAA SG
Sbjct: 186 WRACKNAASSG 196


>ref|XP_019238225.1| PREDICTED: uncharacterized protein LOC109218326 isoform X1
           [Nicotiana attenuata]
          Length = 266

 Score = 58.9 bits (141), Expect = 2e-07
 Identities = 55/191 (28%), Positives = 78/191 (40%), Gaps = 51/191 (26%)
 Frame = -1

Query: 443 QQQPWTLSLRAKAKNLDFKFKLSRILPVSKLFRFSVQFRVHPYSLQIKSK-PKSSFSLNP 267
           Q   W L+++AKAKNLDFK   S  LP  K F FS++F+   + + +KS+ P        
Sbjct: 6   QCHSWKLNVQAKAKNLDFKLVASNFLPNCKFFPFSLKFKYCRFFIHLKSETPTFPNPQKS 65

Query: 266 KSLKLKFLRIFKNFHV-KRSKNKGIARSWFPHK------------------NYFNGRRKR 144
           KSLK K  R  +   + +RSKN   A S  P                         R K 
Sbjct: 66  KSLKSKLHRFLERLRIRRRSKNNSRAISSKPKNKATISQSSVVVGNLALFIQSLYQRDKD 125

Query: 143 CIWL---------------------WVLLFVMVGCFII------MRLSSFLIWKCV---- 57
            I++                     W+LL  ++G  ++      +R SS+    C+    
Sbjct: 126 GIFILGSTVFICFASIFKSFMARKSWILLVFVIGAIVLCSNVMHLRFSSYFDHHCLSRFF 185

Query: 56  WRAWNNAAPSG 24
           WRA  NAA SG
Sbjct: 186 WRACKNAASSG 196