BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia30_contig00021224
         (690 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020553764.1| leucine carboxyl methyltransferase 1 [Sesamu...   295   2e-97
ref|XP_012831473.1| PREDICTED: tRNA wybutosine-synthesizing prot...   292   1e-95
gb|PIN22782.1| Carboxymethyl transferase [Handroanthus impetigin...   289   2e-94
ref|XP_012831475.1| PREDICTED: tRNA wybutosine-synthesizing prot...   286   1e-93
gb|PHU29567.1| hypothetical protein BC332_01660 [Capsicum chinense]   273   1e-88
gb|PHT60268.1| hypothetical protein CQW23_02631 [Capsicum baccatum]   273   3e-88
ref|XP_016544995.1| PREDICTED: tRNA wybutosine-synthesizing prot...   272   7e-88
ref|XP_012083696.1| tRNA wybutosine-synthesizing protein 4 isofo...   271   3e-87
ref|XP_022002704.1| tRNA wybutosine-synthesizing protein 4 [Heli...   270   3e-87
ref|XP_016194165.1| tRNA wybutosine-synthesizing protein 4 [Arac...   270   4e-87
ref|XP_015962199.1| tRNA wybutosine-synthesizing protein 4 [Arac...   270   4e-87
ref|XP_019244375.1| PREDICTED: tRNA wybutosine-synthesizing prot...   270   5e-87
ref|XP_020203493.1| tRNA wybutosine-synthesizing protein 4 [Caja...   267   7e-86
ref|XP_021806697.1| tRNA wybutosine-synthesizing protein 4 [Prun...   267   8e-86
ref|XP_006347961.1| PREDICTED: tRNA wybutosine-synthesizing prot...   267   8e-86
ref|XP_009796080.1| PREDICTED: tRNA wybutosine-synthesizing prot...   265   9e-86
ref|XP_009796078.1| PREDICTED: tRNA wybutosine-synthesizing prot...   266   1e-85
ref|XP_009624471.1| PREDICTED: tRNA wybutosine-synthesizing prot...   265   1e-85
ref|XP_007218225.1| tRNA wybutosine-synthesizing protein 4 [Prun...   266   2e-85
ref|XP_009624470.1| PREDICTED: tRNA wybutosine-synthesizing prot...   266   2e-85

>ref|XP_020553764.1| leucine carboxyl methyltransferase 1 [Sesamum indicum]
          Length = 306

 Score =  295 bits (756), Expect = 2e-97
 Identities = 140/152 (92%), Positives = 144/152 (94%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDPESSRAIVGWASRTF TA+FFLYEQI PDDAFGQQMIRNLESRGCALLGI
Sbjct: 153 IAECVLIYLDPESSRAIVGWASRTFSTAVFFLYEQIRPDDAFGQQMIRNLESRGCALLGI 212

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTLHAKERLFLDQGWQRAVAWDM KVYSTFIEAQERRRIERLELFDEFEEWYMMQEH
Sbjct: 213 YATPTLHAKERLFLDQGWQRAVAWDMQKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 272

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSSTET 52
           YCVACAIND +GLF+NFGFPNE+N TGS T T
Sbjct: 273 YCVACAINDTLGLFKNFGFPNEDNATGSPTAT 304



 Score = 81.6 bits (200), Expect = 1e-14
 Identities = 43/69 (62%), Positives = 51/69 (73%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAA+IENS +LRSK+ EAASIS EKGEVL DHYKLLPV           ISLA+I
Sbjct: 85  EVTSKKAAIIENSTQLRSKISEAASISQEKGEVLADHYKLLPVDLCDIQKLDEIISLANI 144

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 145 DPSLPTFII 153


>ref|XP_012831473.1| PREDICTED: tRNA wybutosine-synthesizing protein 4 isoform X1
           [Erythranthe guttata]
 gb|EYU42345.1| hypothetical protein MIMGU_mgv1a009401mg [Erythranthe guttata]
          Length = 343

 Score =  292 bits (747), Expect = 1e-95
 Identities = 139/149 (93%), Positives = 142/149 (95%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSRAIVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI
Sbjct: 187 IAECVLIYLDPDSSRAIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 246

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           Y TPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH
Sbjct: 247 YDTPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 306

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSS 61
           YCV CAINDA G+F  FGFPNEE+VTGSS
Sbjct: 307 YCVVCAINDASGIFEKFGFPNEEHVTGSS 335



 Score = 76.6 bits (187), Expect = 1e-12
 Identities = 40/69 (57%), Positives = 52/69 (75%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KK +LIE+S +LR++VGE+ASIS EKGEVLGDHYKL+PV           ISLA+I
Sbjct: 119 EVTSKKTSLIESSNQLRARVGESASISTEKGEVLGDHYKLIPVDLREIEKLDDLISLANI 178

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 179 DPSLPTFII 187


>gb|PIN22782.1| Carboxymethyl transferase [Handroanthus impetiginosus]
          Length = 340

 Score =  289 bits (739), Expect = 2e-94
 Identities = 137/152 (90%), Positives = 143/152 (94%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDPESSR IVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI
Sbjct: 187 IAECVLIYLDPESSRDIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 246

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFI+AQERRRIERLELFDEFEEWYMMQEH
Sbjct: 247 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIDAQERRRIERLELFDEFEEWYMMQEH 306

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSSTET 52
           YCV CAIND +G+F  FGFPN +++TGSST T
Sbjct: 307 YCVTCAINDTLGVFDKFGFPNGDHMTGSSTAT 338



 Score = 84.3 bits (207), Expect = 2e-15
 Identities = 45/69 (65%), Positives = 53/69 (76%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAA+IENS +LRSKVGEAASIS +KGEVLGDHYKLLPV           ISLA+I
Sbjct: 119 EVTSKKAAIIENSNQLRSKVGEAASISQDKGEVLGDHYKLLPVDLRDIQKLDDVISLANI 178

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 179 DPSLPTFII 187


>ref|XP_012831475.1| PREDICTED: tRNA wybutosine-synthesizing protein 4 isoform X2
           [Erythranthe guttata]
          Length = 336

 Score =  286 bits (733), Expect = 1e-93
 Identities = 136/146 (93%), Positives = 139/146 (95%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSRAIVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI
Sbjct: 187 IAECVLIYLDPDSSRAIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 246

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           Y TPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH
Sbjct: 247 YDTPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 306

Query: 147 YCVACAINDAMGLFRNFGFPNEENVT 70
           YCV CAINDA G+F  FGFPNEE+VT
Sbjct: 307 YCVVCAINDASGIFEKFGFPNEEHVT 332



 Score = 76.6 bits (187), Expect = 1e-12
 Identities = 40/69 (57%), Positives = 52/69 (75%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KK +LIE+S +LR++VGE+ASIS EKGEVLGDHYKL+PV           ISLA+I
Sbjct: 119 EVTSKKTSLIESSNQLRARVGESASISTEKGEVLGDHYKLIPVDLREIEKLDDLISLANI 178

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 179 DPSLPTFII 187


>gb|PHU29567.1| hypothetical protein BC332_01660 [Capsicum chinense]
          Length = 309

 Score =  273 bits (698), Expect = 1e-88
 Identities = 128/152 (84%), Positives = 138/152 (90%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSRAIVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGC LLGI
Sbjct: 156 IAECVLIYLDPDSSRAIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCGLLGI 215

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTL  KE LF+DQGWQ+AVAWDML+VYS FIEAQERRRIERLE+FDEFEEWYMMQEH
Sbjct: 216 YATPTLQEKENLFVDQGWQKAVAWDMLRVYSDFIEAQERRRIERLEIFDEFEEWYMMQEH 275

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSSTET 52
           YCV  A+NDAMGLF+ FGFPN++  T +ST T
Sbjct: 276 YCVTYAVNDAMGLFKEFGFPNKQPTTNTSTAT 307



 Score = 69.3 bits (168), Expect = 3e-10
 Identities = 38/69 (55%), Positives = 46/69 (66%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAALIE   +LR KVGE ASIS EKGEV  DHYKLLPV           I+ A++
Sbjct: 88  EVTSKKAALIETYSQLRDKVGETASISSEKGEVHSDHYKLLPVDLRNIENLNDIIAQANL 147

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 148 DPSLPTFII 156


>gb|PHT60268.1| hypothetical protein CQW23_02631 [Capsicum baccatum]
          Length = 344

 Score =  273 bits (698), Expect = 3e-88
 Identities = 128/152 (84%), Positives = 138/152 (90%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSRAIVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGC LLGI
Sbjct: 191 IAECVLIYLDPDSSRAIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCGLLGI 250

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTL  KE LF+DQGWQ+AVAWDML+VYS FIEAQERRRIERLE+FDEFEEWYMMQEH
Sbjct: 251 YATPTLQEKENLFVDQGWQKAVAWDMLRVYSDFIEAQERRRIERLEIFDEFEEWYMMQEH 310

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSSTET 52
           YCV  A+NDAMGLF+ FGFPN++  T +ST T
Sbjct: 311 YCVTYAVNDAMGLFKEFGFPNKQPTTNTSTAT 342



 Score = 69.3 bits (168), Expect = 4e-10
 Identities = 38/69 (55%), Positives = 46/69 (66%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAALIE   +LR KVGE ASIS EKGEV  DHYKLLPV           I+ A++
Sbjct: 123 EVTSKKAALIETYSQLRDKVGETASISSEKGEVHSDHYKLLPVDLRNIENLNDIIAQANL 182

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 183 DPSLPTFII 191


>ref|XP_016544995.1| PREDICTED: tRNA wybutosine-synthesizing protein 4 [Capsicum annuum]
 gb|PHT95371.1| hypothetical protein T459_03253 [Capsicum annuum]
          Length = 344

 Score =  272 bits (696), Expect = 7e-88
 Identities = 128/152 (84%), Positives = 138/152 (90%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSRAIVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGC LLGI
Sbjct: 191 IAECVLIYLDPDSSRAIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCGLLGI 250

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTL  KE LF+DQGWQ+AVAWDML+VYS FIEAQE+RRIERLE+FDEFEEWYMMQEH
Sbjct: 251 YATPTLQEKENLFVDQGWQKAVAWDMLRVYSDFIEAQEKRRIERLEIFDEFEEWYMMQEH 310

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSSTET 52
           YCV  AINDAMGLF+ FGFPN++  T +ST T
Sbjct: 311 YCVTYAINDAMGLFKEFGFPNKQPTTNTSTAT 342



 Score = 69.3 bits (168), Expect = 4e-10
 Identities = 38/69 (55%), Positives = 46/69 (66%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAALIE   +LR KVGE ASIS EKGEV  DHYKLLPV           I+ A++
Sbjct: 123 EVTSKKAALIETYSQLRDKVGETASISSEKGEVHSDHYKLLPVDLRNIENLNDIIAQANL 182

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 183 DPSLPTFII 191


>ref|XP_012083696.1| tRNA wybutosine-synthesizing protein 4 isoform X1 [Jatropha curcas]
 gb|KDP28857.1| hypothetical protein JCGZ_14628 [Jatropha curcas]
          Length = 342

 Score =  271 bits (692), Expect = 3e-87
 Identities = 128/143 (89%), Positives = 134/143 (93%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+S+RAIVGWASRTF TA+FFLYEQIHPDDAFGQQMIRNLESRGCALLGI
Sbjct: 188 IAECVLIYLDPDSTRAIVGWASRTFSTAVFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 247

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           Y TPTL AKERLFLDQGWQR VAWDML+VYS FIEAQERRRIERLELFDEFEEW+MMQEH
Sbjct: 248 YDTPTLLAKERLFLDQGWQRVVAWDMLRVYSDFIEAQERRRIERLELFDEFEEWHMMQEH 307

Query: 147 YCVACAINDAMGLFRNFGFPNEE 79
           YCVACAINDAMGLF NFGFP  +
Sbjct: 308 YCVACAINDAMGLFGNFGFPTHQ 330



 Score = 72.4 bits (176), Expect = 3e-11
 Identities = 38/69 (55%), Positives = 50/69 (72%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAA+IE+  +LR KVG  +SIS EKGEVLGD+YKLLPV           I+LA++
Sbjct: 120 EVTSKKAAIIESYSQLRDKVGATSSISREKGEVLGDNYKLLPVDLRDIQKLDDIIALANM 179

Query: 508 DPRMCSYIL 482
           DPR+ ++I+
Sbjct: 180 DPRLPTFII 188


>ref|XP_022002704.1| tRNA wybutosine-synthesizing protein 4 [Helianthus annuus]
 gb|OTG03361.1| putative leucine carboxyl methyltransferase [Helianthus annuus]
          Length = 339

 Score =  270 bits (691), Expect = 3e-87
 Identities = 125/150 (83%), Positives = 139/150 (92%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           + ECVLIYLDP+SS AIV WASRTFPT++FFLYEQI+P DAFGQQMIRNLESRGC LLGI
Sbjct: 186 VAECVLIYLDPDSSNAIVEWASRTFPTSVFFLYEQINPHDAFGQQMIRNLESRGCGLLGI 245

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTL AKE LFLD+GWQRAVAWDMLK+Y++FIE QER+RIERLELFDEFEEWYMMQEH
Sbjct: 246 YATPTLLAKENLFLDRGWQRAVAWDMLKIYTSFIEPQERQRIERLELFDEFEEWYMMQEH 305

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSST 58
           YCVACAINDAMG+F NFGFP ++ VTG+ST
Sbjct: 306 YCVACAINDAMGMFENFGFPKDQPVTGAST 335



 Score = 57.8 bits (138), Expect = 4e-06
 Identities = 27/43 (62%), Positives = 33/43 (76%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPV 560
           EVT KKA +IENS +LR K+ E A+I  EKGEV+ D YKLLP+
Sbjct: 119 EVTCKKATIIENSPQLRGKISEPATICQEKGEVISDRYKLLPI 161


>ref|XP_016194165.1| tRNA wybutosine-synthesizing protein 4 [Arachis ipaensis]
          Length = 335

 Score =  270 bits (690), Expect = 4e-87
 Identities = 126/149 (84%), Positives = 141/149 (94%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+S++A+VGWAS+TF TA FFLYEQIHPDDAFGQQM+RNLESRGCALLGI
Sbjct: 187 IAECVLIYLDPDSTQAVVGWASQTFSTAAFFLYEQIHPDDAFGQQMMRNLESRGCALLGI 246

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           +ATPTLHAKE+LFLDQGWQR+VAWDML++Y+ FI+AQERRRIERLELFDEFEEWYMMQEH
Sbjct: 247 HATPTLHAKEKLFLDQGWQRSVAWDMLRIYNDFIDAQERRRIERLELFDEFEEWYMMQEH 306

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSS 61
           YCVA AINDAMGLFR+FGF NEE+V  SS
Sbjct: 307 YCVAIAINDAMGLFRDFGFINEESVPHSS 335



 Score = 71.6 bits (174), Expect = 6e-11
 Identities = 37/69 (53%), Positives = 47/69 (68%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KK ALIE S +LR+KVGEAA IS EKGEVL DHYKL+P            ++ A +
Sbjct: 119 EVTSKKTALIETSNQLRNKVGEAAVISREKGEVLSDHYKLIPADLRDRQQLSDIVTRADM 178

Query: 508 DPRMCSYIL 482
           DPR+ ++I+
Sbjct: 179 DPRLPTFII 187


>ref|XP_015962199.1| tRNA wybutosine-synthesizing protein 4 [Arachis duranensis]
          Length = 335

 Score =  270 bits (690), Expect = 4e-87
 Identities = 126/149 (84%), Positives = 141/149 (94%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+S++A+VGWAS+TF TA FFLYEQIHPDDAFGQQM+RNLESRGCALLGI
Sbjct: 187 IAECVLIYLDPDSTQAVVGWASQTFSTAAFFLYEQIHPDDAFGQQMMRNLESRGCALLGI 246

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           +ATPTLHAKE+LFLDQGWQR+VAWDML++Y+ FI+AQERRRIERLELFDEFEEWYMMQEH
Sbjct: 247 HATPTLHAKEKLFLDQGWQRSVAWDMLRIYNDFIDAQERRRIERLELFDEFEEWYMMQEH 306

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSS 61
           YCVA AINDAMGLFR+FGF NEE+V  SS
Sbjct: 307 YCVAIAINDAMGLFRDFGFINEESVPHSS 335



 Score = 71.6 bits (174), Expect = 6e-11
 Identities = 37/69 (53%), Positives = 47/69 (68%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KK ALIE S +LR+KVGEAA IS EKGEVL DHYKL+P            ++ A +
Sbjct: 119 EVTSKKTALIETSNQLRNKVGEAAVISREKGEVLSDHYKLIPADLRDRQQLSDIVARADM 178

Query: 508 DPRMCSYIL 482
           DPR+ ++I+
Sbjct: 179 DPRLPTFII 187


>ref|XP_019244375.1| PREDICTED: tRNA wybutosine-synthesizing protein 4 isoform X2
           [Nicotiana attenuata]
 gb|OIT07771.1| hypothetical protein A4A49_00272 [Nicotiana attenuata]
          Length = 341

 Score =  270 bits (690), Expect = 5e-87
 Identities = 128/152 (84%), Positives = 136/152 (89%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSR IVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGC LLGI
Sbjct: 188 IAECVLIYLDPDSSRTIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCGLLGI 247

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTL  KE LFLDQGWQ+AVAWDML+VYS FIEAQERRRIERLE+FDEFEEWYMMQEH
Sbjct: 248 YATPTLQEKENLFLDQGWQKAVAWDMLRVYSDFIEAQERRRIERLEIFDEFEEWYMMQEH 307

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSSTET 52
           YCV  AINDAMGLF+ FGFPN + +T +S  T
Sbjct: 308 YCVTYAINDAMGLFKEFGFPNTQPMTDTSIAT 339



 Score = 69.3 bits (168), Expect = 4e-10
 Identities = 38/69 (55%), Positives = 46/69 (66%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAALIE   +LR KVGE ASIS EKGEV  DHYKLLPV           I+ A++
Sbjct: 120 EVTSKKAALIETYSQLRDKVGETASISSEKGEVHSDHYKLLPVDLRNIDNLNDVIARANL 179

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 180 DPSLPTFII 188


>ref|XP_020203493.1| tRNA wybutosine-synthesizing protein 4 [Cajanus cajan]
          Length = 337

 Score =  267 bits (682), Expect = 7e-86
 Identities = 124/145 (85%), Positives = 137/145 (94%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSRA+VGWAS+TF TA+FFLYEQI PDDAFGQQM+RNLESRGCALLGI
Sbjct: 187 ISECVLIYLDPDSSRAVVGWASQTFTTAVFFLYEQILPDDAFGQQMMRNLESRGCALLGI 246

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTLHAKE+LFLDQGWQ++VAWDML+VY+ FI+AQERRRIERLELFDEFEEWYMMQEH
Sbjct: 247 YATPTLHAKEKLFLDQGWQKSVAWDMLRVYNDFIDAQERRRIERLELFDEFEEWYMMQEH 306

Query: 147 YCVACAINDAMGLFRNFGFPNEENV 73
           YCVA AIND MGLFRNFGF N++ V
Sbjct: 307 YCVAYAINDGMGLFRNFGFVNDKTV 331



 Score = 65.1 bits (157), Expect = 1e-08
 Identities = 34/69 (49%), Positives = 46/69 (66%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAALIE   +LR+K+GE A+IS EKGEV+  HYKL+P            IS+A +
Sbjct: 119 EVTSKKAALIETHSQLRNKLGELATISREKGEVVSAHYKLVPADLRYVQQLNDIISVAGM 178

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 179 DPSLPTFII 187


>ref|XP_021806697.1| tRNA wybutosine-synthesizing protein 4 [Prunus avium]
          Length = 351

 Score =  267 bits (683), Expect = 8e-86
 Identities = 126/143 (88%), Positives = 133/143 (93%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSRAIVGW S+ F TAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI
Sbjct: 188 IAECVLIYLDPDSSRAIVGWTSKAFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 247

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           + TPTL AKE+LFLDQGWQRAVAWDMLKVYS F+EAQERRRIERLELFDEFEEWYMMQEH
Sbjct: 248 HDTPTLQAKEKLFLDQGWQRAVAWDMLKVYSNFVEAQERRRIERLELFDEFEEWYMMQEH 307

Query: 147 YCVACAINDAMGLFRNFGFPNEE 79
           YCVA AINDAMGLF +FGFPN +
Sbjct: 308 YCVAYAINDAMGLFEDFGFPNNQ 330



 Score = 63.5 bits (153), Expect = 4e-08
 Identities = 34/69 (49%), Positives = 42/69 (60%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KK  LIE    LR K+G  ASIS EKGEVL DHYKLLP+           I+LA +
Sbjct: 120 EVTSKKTTLIETCNHLRDKIGATASISQEKGEVLSDHYKLLPIDLRNIQQLDDVIALAGM 179

Query: 508 DPRMCSYIL 482
           D  + ++I+
Sbjct: 180 DRSLPTFII 188


>ref|XP_006347961.1| PREDICTED: tRNA wybutosine-synthesizing protein 4 [Solanum
           tuberosum]
          Length = 341

 Score =  267 bits (682), Expect = 8e-86
 Identities = 127/152 (83%), Positives = 134/152 (88%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSRAIVGWASRTF T+IFFLYEQIHPDDAFGQQMIRNLESRGC LLGI
Sbjct: 188 IAECVLIYLDPDSSRAIVGWASRTFSTSIFFLYEQIHPDDAFGQQMIRNLESRGCGLLGI 247

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTL  KE LFLDQGWQ+AVAWDML+VYS FIEAQERRRIERLE+FDEFEEWYMMQEH
Sbjct: 248 YATPTLQEKENLFLDQGWQKAVAWDMLQVYSDFIEAQERRRIERLEIFDEFEEWYMMQEH 307

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSSTET 52
           YCV  AINDAMGLF+ FGFP     T +S  T
Sbjct: 308 YCVTYAINDAMGLFKEFGFPKTHPTTNTSIAT 339



 Score = 69.3 bits (168), Expect = 4e-10
 Identities = 38/69 (55%), Positives = 46/69 (66%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAALIE   +LR KVGE ASIS EKGEV  DHYKLLPV           I+ A++
Sbjct: 120 EVTSKKAALIETYSQLRDKVGETASISSEKGEVHSDHYKLLPVDLRNIENLNDIIAQANL 179

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 180 DPSLPTFII 188


>ref|XP_009796080.1| PREDICTED: tRNA wybutosine-synthesizing protein 4 isoform X3
           [Nicotiana sylvestris]
          Length = 299

 Score =  265 bits (678), Expect = 9e-86
 Identities = 126/150 (84%), Positives = 134/150 (89%)
 Frame = -1

Query: 501 ECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGIYA 322
           ECVLIYLDP+SSR IVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGC LLGIYA
Sbjct: 148 ECVLIYLDPDSSRTIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCGLLGIYA 207

Query: 321 TPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEHYC 142
           TPTL  KE LFLDQGWQ+AVAWDML+VYS FIEAQERRRIERLE+FDEFEEWYMMQEHYC
Sbjct: 208 TPTLQEKENLFLDQGWQKAVAWDMLRVYSDFIEAQERRRIERLEIFDEFEEWYMMQEHYC 267

Query: 141 VACAINDAMGLFRNFGFPNEENVTGSSTET 52
           V  AINDAM LF+ FGFPN + +T +S  T
Sbjct: 268 VTYAINDAMCLFKEFGFPNTQPMTDTSIAT 297


>ref|XP_009796078.1| PREDICTED: tRNA wybutosine-synthesizing protein 4 isoform X1
           [Nicotiana sylvestris]
 ref|XP_016510297.1| PREDICTED: tRNA wybutosine-synthesizing protein 4-like isoform X1
           [Nicotiana tabacum]
          Length = 341

 Score =  266 bits (681), Expect = 1e-85
 Identities = 127/152 (83%), Positives = 135/152 (88%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSR IVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGC LLGI
Sbjct: 188 IAECVLIYLDPDSSRTIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCGLLGI 247

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTL  KE LFLDQGWQ+AVAWDML+VYS FIEAQERRRIERLE+FDEFEEWYMMQEH
Sbjct: 248 YATPTLQEKENLFLDQGWQKAVAWDMLRVYSDFIEAQERRRIERLEIFDEFEEWYMMQEH 307

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSSTET 52
           YCV  AINDAM LF+ FGFPN + +T +S  T
Sbjct: 308 YCVTYAINDAMCLFKEFGFPNTQPMTDTSIAT 339



 Score = 68.9 bits (167), Expect = 5e-10
 Identities = 38/69 (55%), Positives = 46/69 (66%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAALIE   +LR KVGE ASIS EKGEV  DHYKLLPV           I+ A++
Sbjct: 120 EVTSKKAALIETYSQLRDKVGEIASISSEKGEVHSDHYKLLPVDLRNIDNLNDVIARANL 179

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 180 DPSLPTFII 188


>ref|XP_009624471.1| PREDICTED: tRNA wybutosine-synthesizing protein 4 isoform X3
           [Nicotiana tomentosiformis]
          Length = 299

 Score =  265 bits (677), Expect = 1e-85
 Identities = 125/150 (83%), Positives = 133/150 (88%)
 Frame = -1

Query: 501 ECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGIYA 322
           ECVLIYLDP+SSR IVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGC LLGIYA
Sbjct: 148 ECVLIYLDPDSSRTIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCGLLGIYA 207

Query: 321 TPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEHYC 142
           TPTL  KE LFLDQGWQ+AVAWDML+VYS FIEAQERRRIERLE+FDEFEEWYMMQEHYC
Sbjct: 208 TPTLQEKENLFLDQGWQKAVAWDMLRVYSDFIEAQERRRIERLEIFDEFEEWYMMQEHYC 267

Query: 141 VACAINDAMGLFRNFGFPNEENVTGSSTET 52
           V  AIND MG+F+ FGFPN + +T  S  T
Sbjct: 268 VTYAINDPMGMFKEFGFPNTQPMTDISIAT 297


>ref|XP_007218225.1| tRNA wybutosine-synthesizing protein 4 [Prunus persica]
 gb|ONI22463.1| hypothetical protein PRUPE_2G131200 [Prunus persica]
          Length = 351

 Score =  266 bits (681), Expect = 2e-85
 Identities = 126/143 (88%), Positives = 132/143 (92%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSRAIVGW S+ F TAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI
Sbjct: 188 IAECVLIYLDPDSSRAIVGWTSKAFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 247

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           + TPTL AKE LFLDQGWQRAVAWDMLKVYS F+EAQERRRIERLELFDEFEEWYMMQEH
Sbjct: 248 HDTPTLQAKENLFLDQGWQRAVAWDMLKVYSNFVEAQERRRIERLELFDEFEEWYMMQEH 307

Query: 147 YCVACAINDAMGLFRNFGFPNEE 79
           YCVA AINDAMGLF +FGFPN +
Sbjct: 308 YCVAYAINDAMGLFEDFGFPNNQ 330



 Score = 60.5 bits (145), Expect = 4e-07
 Identities = 32/69 (46%), Positives = 41/69 (59%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KK  LIE    L+ K+   ASIS EKGEVL DHYKLLP+           I+LA +
Sbjct: 120 EVTSKKTTLIETCSHLKDKISATASISREKGEVLSDHYKLLPIDLRNIQQLDDVIALAGM 179

Query: 508 DPRMCSYIL 482
           D  + ++I+
Sbjct: 180 DRSLPTFII 188


>ref|XP_009624470.1| PREDICTED: tRNA wybutosine-synthesizing protein 4 isoform X1
           [Nicotiana tomentosiformis]
 ref|XP_016439467.1| PREDICTED: tRNA wybutosine-synthesizing protein 4-like [Nicotiana
           tabacum]
          Length = 341

 Score =  266 bits (680), Expect = 2e-85
 Identities = 126/152 (82%), Positives = 134/152 (88%)
 Frame = -1

Query: 507 IQECVLIYLDPESSRAIVGWASRTFPTAIFFLYEQIHPDDAFGQQMIRNLESRGCALLGI 328
           I ECVLIYLDP+SSR IVGWASRTF TAIFFLYEQIHPDDAFGQQMIRNLESRGC LLGI
Sbjct: 188 IAECVLIYLDPDSSRTIVGWASRTFSTAIFFLYEQIHPDDAFGQQMIRNLESRGCGLLGI 247

Query: 327 YATPTLHAKERLFLDQGWQRAVAWDMLKVYSTFIEAQERRRIERLELFDEFEEWYMMQEH 148
           YATPTL  KE LFLDQGWQ+AVAWDML+VYS FIEAQERRRIERLE+FDEFEEWYMMQEH
Sbjct: 248 YATPTLQEKENLFLDQGWQKAVAWDMLRVYSDFIEAQERRRIERLEIFDEFEEWYMMQEH 307

Query: 147 YCVACAINDAMGLFRNFGFPNEENVTGSSTET 52
           YCV  AIND MG+F+ FGFPN + +T  S  T
Sbjct: 308 YCVTYAINDPMGMFKEFGFPNTQPMTDISIAT 339



 Score = 69.3 bits (168), Expect = 4e-10
 Identities = 38/69 (55%), Positives = 46/69 (66%)
 Frame = -3

Query: 688 EVTRKKAALIENSVELRSKVGEAASISHEKGEVLGDHYKLLPVXXXXXXXXXXXISLASI 509
           EVT KKAALIE   +LR KVGE ASIS EKGEV  DHYKLLPV           I+ A++
Sbjct: 120 EVTSKKAALIETYSQLRDKVGETASISSEKGEVHSDHYKLLPVDLRNIDNLNDIIARANL 179

Query: 508 DPRMCSYIL 482
           DP + ++I+
Sbjct: 180 DPSLPTFII 188