BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia30_contig00002097
         (1363 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PIN03577.1| 1,4-alpha-glucan branching enzyme/starch branchin...   716   0.0  
ref|XP_011094068.1| isoamylase 1, chloroplastic isoform X2 [Sesa...   714   0.0  
ref|XP_011094067.1| isoamylase 1, chloroplastic isoform X1 [Sesa...   714   0.0  
ref|XP_012851211.1| PREDICTED: isoamylase 1, chloroplastic [Eryt...   701   0.0  
ref|XP_022850845.1| isoamylase 1, chloroplastic [Olea europaea v...   667   0.0  
gb|KZV34472.1| isoamylase isoform 1 [Dorcoceras hygrometricum]        621   0.0  
ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum] >gi...   591   0.0  
ref|XP_019234068.1| PREDICTED: isoamylase 1, chloroplastic [Nico...   591   0.0  
ref|XP_015082414.1| PREDICTED: isoamylase 1, chloroplastic isofo...   587   0.0  
dbj|BAW33106.1| isoamylase [Ipomoea batatas]                          587   0.0  
ref|XP_016580325.1| PREDICTED: isoamylase 1, chloroplastic-like ...   574   0.0  
ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic isofo...   583   0.0  
gb|EPS64233.1| isoamylase isoform 1, partial [Genlisea aurea]         582   0.0  
gb|AAY84833.1| isoamylase [Ipomoea batatas]                           582   0.0  
gb|PHT75937.1| Isoamylase 1, chloroplastic [Capsicum annuum]          583   0.0  
ref|XP_010323372.1| PREDICTED: isoamylase 1, chloroplastic isofo...   583   0.0  
ref|XP_009588914.1| PREDICTED: isoamylase 1, chloroplastic [Nico...   582   0.0  
ref|XP_019188755.1| PREDICTED: isoamylase 1, chloroplastic [Ipom...   582   0.0  
ref|XP_009771255.1| PREDICTED: isoamylase 1, chloroplastic [Nico...   581   0.0  
ref|XP_016434247.1| PREDICTED: isoamylase 1, chloroplastic-like ...   580   0.0  

>gb|PIN03577.1| 1,4-alpha-glucan branching enzyme/starch branching enzyme II
            [Handroanthus impetiginosus]
          Length = 798

 Score =  716 bits (1847), Expect = 0.0
 Identities = 336/407 (82%), Positives = 364/407 (89%)
 Frame = +3

Query: 141  MELQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKR 320
            MEL LLH+S  P+YCPPKFT  KYL+C KNKLN G+PSK F NTG   N  VILNNAS+R
Sbjct: 1    MELYLLHFSLTPNYCPPKFTSTKYLKCWKNKLNAGLPSKSFANTGTKANSFVILNNASER 60

Query: 321  GDTETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDL 500
            GD ETAVVVEKPP   RRFE F GHPTPFGATPRDGGV            +LCLISLSDL
Sbjct: 61   GDAETAVVVEKPPPRPRRFEFFPGHPTPFGATPRDGGVNFAVFSSTASSASLCLISLSDL 120

Query: 501  REKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDP 680
             EKRVT++IAL+PLTNKTGDVWHVFLKGDFEDM YGY+F+GSFSPEDG YFDSSK+LIDP
Sbjct: 121  PEKRVTKEIALNPLTNKTGDVWHVFLKGDFEDMVYGYRFEGSFSPEDGLYFDSSKILIDP 180

Query: 681  YAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGF 860
            YAKA+V RGEYG LGPD++CWPQMA +VPST+DEFDWEGDLPLK PQ +LVIYEMHVRGF
Sbjct: 181  YAKAIVSRGEYGTLGPDEECWPQMASVVPSTSDEFDWEGDLPLKFPQRELVIYEMHVRGF 240

Query: 861  TRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFW 1040
            TRHESSNSK+PGTYLG+VEKLD+LKKLGVNCIELMPCH+FNELE+YSYN+VLGD KMNFW
Sbjct: 241  TRHESSNSKFPGTYLGVVEKLDYLKKLGVNCIELMPCHDFNELEYYSYNTVLGDSKMNFW 300

Query: 1041 GYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENG 1220
            GYSTIN+FSPMARYSS+GIGNYGLGAVNEFK LVREAHKRGIEVIMDVVFNHTAEGNE G
Sbjct: 301  GYSTINYFSPMARYSSAGIGNYGLGAVNEFKCLVREAHKRGIEVIMDVVFNHTAEGNEQG 360

Query: 1221 PILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            PILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFI++CL
Sbjct: 361  PILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFIVECL 407


>ref|XP_011094068.1| isoamylase 1, chloroplastic isoform X2 [Sesamum indicum]
          Length = 798

 Score =  714 bits (1844), Expect = 0.0
 Identities = 340/409 (83%), Positives = 362/409 (88%)
 Frame = +3

Query: 135  VEMELQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNAS 314
            ++MELQ L  S IP++   K  PK  LRCRK+KLN GIPSK F N G NVNP VILNNAS
Sbjct: 1    MKMELQSLQSSLIPNHFSRKSAPKTCLRCRKDKLNVGIPSKAFANAGKNVNPFVILNNAS 60

Query: 315  KRGDTETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLS 494
            +R D ETAVVVEKPPA  RRFEVFSG PTPFGAT RDGGV            TLCLI+LS
Sbjct: 61   ERVDAETAVVVEKPPARARRFEVFSGQPTPFGATARDGGVNFAVSSGNASSATLCLINLS 120

Query: 495  DLREKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLI 674
            DL EKRVTEQIAL P  N+TGDVWHVFLKGDFEDM YGY+FDGSFSPEDG YFDSSK+LI
Sbjct: 121  DLPEKRVTEQIALDPTNNRTGDVWHVFLKGDFEDMVYGYRFDGSFSPEDGLYFDSSKILI 180

Query: 675  DPYAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVR 854
            DPYAKAVV RGEYGALGPD++CWPQMAC+VPS NDEFDWEGDLPLK PQ DLVIYEMHVR
Sbjct: 181  DPYAKAVVSRGEYGALGPDEECWPQMACVVPSINDEFDWEGDLPLKFPQRDLVIYEMHVR 240

Query: 855  GFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMN 1034
            GFTRHESSNSK+PGTY G+VEKLDHLKKLG+NCIELMPCHEFNELE+YSYNSVLGDYK+N
Sbjct: 241  GFTRHESSNSKFPGTYRGLVEKLDHLKKLGINCIELMPCHEFNELEYYSYNSVLGDYKVN 300

Query: 1035 FWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNE 1214
            FWGYSTIN+FSPMARYSS+G+G YGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNE
Sbjct: 301  FWGYSTINYFSPMARYSSAGVGKYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNE 360

Query: 1215 NGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            NGPILSFRGLDNSVFYMLAPKGE+YNYSGCGNTFNCNHPIVRQFILDCL
Sbjct: 361  NGPILSFRGLDNSVFYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCL 409


>ref|XP_011094067.1| isoamylase 1, chloroplastic isoform X1 [Sesamum indicum]
          Length = 833

 Score =  714 bits (1844), Expect = 0.0
 Identities = 340/409 (83%), Positives = 362/409 (88%)
 Frame = +3

Query: 135  VEMELQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNAS 314
            ++MELQ L  S IP++   K  PK  LRCRK+KLN GIPSK F N G NVNP VILNNAS
Sbjct: 1    MKMELQSLQSSLIPNHFSRKSAPKTCLRCRKDKLNVGIPSKAFANAGKNVNPFVILNNAS 60

Query: 315  KRGDTETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLS 494
            +R D ETAVVVEKPPA  RRFEVFSG PTPFGAT RDGGV            TLCLI+LS
Sbjct: 61   ERVDAETAVVVEKPPARARRFEVFSGQPTPFGATARDGGVNFAVSSGNASSATLCLINLS 120

Query: 495  DLREKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLI 674
            DL EKRVTEQIAL P  N+TGDVWHVFLKGDFEDM YGY+FDGSFSPEDG YFDSSK+LI
Sbjct: 121  DLPEKRVTEQIALDPTNNRTGDVWHVFLKGDFEDMVYGYRFDGSFSPEDGLYFDSSKILI 180

Query: 675  DPYAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVR 854
            DPYAKAVV RGEYGALGPD++CWPQMAC+VPS NDEFDWEGDLPLK PQ DLVIYEMHVR
Sbjct: 181  DPYAKAVVSRGEYGALGPDEECWPQMACVVPSINDEFDWEGDLPLKFPQRDLVIYEMHVR 240

Query: 855  GFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMN 1034
            GFTRHESSNSK+PGTY G+VEKLDHLKKLG+NCIELMPCHEFNELE+YSYNSVLGDYK+N
Sbjct: 241  GFTRHESSNSKFPGTYRGLVEKLDHLKKLGINCIELMPCHEFNELEYYSYNSVLGDYKVN 300

Query: 1035 FWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNE 1214
            FWGYSTIN+FSPMARYSS+G+G YGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNE
Sbjct: 301  FWGYSTINYFSPMARYSSAGVGKYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNE 360

Query: 1215 NGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            NGPILSFRGLDNSVFYMLAPKGE+YNYSGCGNTFNCNHPIVRQFILDCL
Sbjct: 361  NGPILSFRGLDNSVFYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCL 409


>ref|XP_012851211.1| PREDICTED: isoamylase 1, chloroplastic [Erythranthe guttata]
 gb|EYU25738.1| hypothetical protein MIMGU_mgv1a001549mg [Erythranthe guttata]
          Length = 798

 Score =  701 bits (1808), Expect = 0.0
 Identities = 335/407 (82%), Positives = 357/407 (87%)
 Frame = +3

Query: 141  MELQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKR 320
            ME Q LH   IP+Y  PKF+ KKYLR RKN+LN GI SKPFEN+G N NP + L+ AS R
Sbjct: 1    METQFLHNFTIPNYRSPKFSSKKYLRRRKNELNAGITSKPFENSGTNPNPYITLSTASGR 60

Query: 321  GDTETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDL 500
            GD ETAVVVEKPP     FEVFSG+P PFGATP DGGV            TLCLI+LSDL
Sbjct: 61   GDAETAVVVEKPPPKPPSFEVFSGNPMPFGATPGDGGVNFSVVSSNASSATLCLITLSDL 120

Query: 501  REKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDP 680
            +E+RVTEQIAL+PLTNKTGDVWHVFLKGDFEDM Y Y+FDGSF PEDG YFDSSK+LIDP
Sbjct: 121  QERRVTEQIALNPLTNKTGDVWHVFLKGDFEDMVYAYRFDGSFCPEDGLYFDSSKLLIDP 180

Query: 681  YAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGF 860
            YAKAVV RGEYGALG   +CWPQMACLVPST DEFDWEGDLPLKLPQ DL +YEMHVRGF
Sbjct: 181  YAKAVVSRGEYGALGTGDECWPQMACLVPSTTDEFDWEGDLPLKLPQRDLFMYEMHVRGF 240

Query: 861  TRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFW 1040
            TRHESSNSKYPGTYLG+VEKLD+LKKLGVNCIELMPCHEFNELE+YSYN VLGDYKMNFW
Sbjct: 241  TRHESSNSKYPGTYLGVVEKLDYLKKLGVNCIELMPCHEFNELEYYSYNPVLGDYKMNFW 300

Query: 1041 GYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENG 1220
            GYSTIN+FSPMARYSSSGIGNYG+GAVN+FKYLVREAHKRGIEVIMDVVFNHTAEGNENG
Sbjct: 301  GYSTINYFSPMARYSSSGIGNYGIGAVNDFKYLVREAHKRGIEVIMDVVFNHTAEGNENG 360

Query: 1221 PILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            PILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFI+D L
Sbjct: 361  PILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFIVDSL 407


>ref|XP_022850845.1| isoamylase 1, chloroplastic [Olea europaea var. sylvestris]
          Length = 793

 Score =  667 bits (1722), Expect = 0.0
 Identities = 311/403 (77%), Positives = 350/403 (86%)
 Frame = +3

Query: 153  LLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKRGDTE 332
            LLH S + S C PKF P       K+K+NFG+  K FEN+    N ++I+N+A+ RGD E
Sbjct: 3    LLHCSSL-SNCTPKFPPTNSFHFGKSKINFGVSQKSFENSVRKPNSLLIVNDATDRGDAE 61

Query: 333  TAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLREKR 512
            T+VVVEKPP  LRRFEVFSGHP PFGAT RDGGV            TLCLI+LSDL EKR
Sbjct: 62   TSVVVEKPPPKLRRFEVFSGHPMPFGATLRDGGVNFAISSSNATSATLCLINLSDLSEKR 121

Query: 513  VTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYAKA 692
            VTEQI+L+PLTNKTGDVWHVFL GDFE+M YGYKFDG FSPE+G YF+SSK+LIDPYAK+
Sbjct: 122  VTEQISLNPLTNKTGDVWHVFLTGDFENMVYGYKFDGEFSPEEGHYFNSSKILIDPYAKS 181

Query: 693  VVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGFTRHE 872
            +V RGEYG +GP++DCWPQ+AC VPS NDEFDWEGDLPL  PQ DL+IYEMHVRG+TRHE
Sbjct: 182  IVSRGEYGTIGPEEDCWPQLACSVPSDNDEFDWEGDLPLNFPQRDLIIYEMHVRGYTRHE 241

Query: 873  SSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGYST 1052
            SS +K PGTYLG+VEKLDHLKKLGVNCIELMPCHEFNELE+YSYNS+LG+YKMNFWGYST
Sbjct: 242  SSKAKCPGTYLGVVEKLDHLKKLGVNCIELMPCHEFNELEYYSYNSILGNYKMNFWGYST 301

Query: 1053 INFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPILS 1232
            +NFFSPMARYSS+GIGNYGLGAVNEFK+LVREAHKRGIEVIMDVVFNHTAEGNENGP++S
Sbjct: 302  VNFFSPMARYSSAGIGNYGLGAVNEFKHLVREAHKRGIEVIMDVVFNHTAEGNENGPVIS 361

Query: 1233 FRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            FRG+DNSVFYMLAPKGE+YNYSGCGNTFNCNHPIVRQFIL+CL
Sbjct: 362  FRGVDNSVFYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILECL 404


>gb|KZV34472.1| isoamylase isoform 1 [Dorcoceras hygrometricum]
          Length = 792

 Score =  621 bits (1602), Expect = 0.0
 Identities = 298/405 (73%), Positives = 332/405 (81%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKRGD 326
            ++LLHY  I S   PKF+  KYLR +K +L+ G+  K   N       ++++NNAS+ G+
Sbjct: 1    MELLHYPSISSI-HPKFSSIKYLRWKK-RLSVGLSGKLSANAKKGFGSLLVMNNASEGGE 58

Query: 327  TETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLRE 506
             +  VVV  P     R EV SG+PTPFGATPRDGG+            TLCLI +SDL E
Sbjct: 59   ADITVVVRPPSTKPWRLEVLSGYPTPFGATPRDGGINFVVSSSNATSATLCLIDISDLPE 118

Query: 507  KRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYA 686
            KRVTE+IAL PLT+KTGDVWHVF+KGD ED+ YGY+FDG FSPEDG YFDSSK+LIDPYA
Sbjct: 119  KRVTEEIALDPLTHKTGDVWHVFVKGDLEDVVYGYRFDGRFSPEDGLYFDSSKILIDPYA 178

Query: 687  KAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGFTR 866
            KAVV RGEYGALG + DCWPQMACLVPS N+EFDWEGDLPL LPQ DLVIYEMHVRGFT 
Sbjct: 179  KAVVSRGEYGALGHNGDCWPQMACLVPSANNEFDWEGDLPLNLPQRDLVIYEMHVRGFTN 238

Query: 867  HESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGY 1046
            HESSNSK+PGTYLG+VEKLDHL+ +G NCIELMPCHEFNELE+YSYN V GDYKMNFWGY
Sbjct: 239  HESSNSKFPGTYLGVVEKLDHLENIGFNCIELMPCHEFNELEYYSYNPVQGDYKMNFWGY 298

Query: 1047 STINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPI 1226
            STINFFSPMARYS +G+GN+GLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGP 
Sbjct: 299  STINFFSPMARYSFAGLGNHGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPT 358

Query: 1227 LSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            LSFRGLDN VFYM AP+GEYYNYSGCGNT NCN PIVRQFILD L
Sbjct: 359  LSFRGLDNLVFYMTAPRGEYYNYSGCGNTLNCNQPIVRQFILDSL 403


>ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum]
 gb|AAN15317.1| isoamylase isoform 1 [Solanum tuberosum]
          Length = 793

 Score =  591 bits (1524), Expect = 0.0
 Identities = 286/405 (70%), Positives = 320/405 (79%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKRGD 326
            ++LLH   I +Y  PK +   +L  R++       S   ++    VN  V          
Sbjct: 1    MELLHCPSISTY-KPKLSFHNHLFSRRSSNGVDFESIWRKSRSSVVNAAVDSGRGGVVKT 59

Query: 327  TETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLRE 506
              TAVVVEKP     RFEV SG P PFGAT  DGGV            TLCLI+LSDL E
Sbjct: 60   AATAVVVEKPTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSDLPE 119

Query: 507  KRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYA 686
            KRVTEQI L PL NKTGDVWHVFLKGDFE+M YGYKFDG F PE+G YFDSS++++DPYA
Sbjct: 120  KRVTEQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLDPYA 179

Query: 687  KAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGFTR 866
            KA+V RGEYG LGP+ DCWP MA +VPS +D+FDWEGDLPLK PQ DLVIYEMHVRGFT 
Sbjct: 180  KAIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFPQRDLVIYEMHVRGFTN 239

Query: 867  HESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGY 1046
            HESS +KYPGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK NFWGY
Sbjct: 240  HESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGY 299

Query: 1047 STINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPI 1226
            ST+NFFSPM RYSS+G+ N GLGA+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNENGPI
Sbjct: 300  STVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPI 359

Query: 1227 LSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            LSFRG+DNSVFY LAPKGE+YNYSGCGNTFNCN+PIVRQFI+DCL
Sbjct: 360  LSFRGIDNSVFYTLAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCL 404


>ref|XP_019234068.1| PREDICTED: isoamylase 1, chloroplastic [Nicotiana attenuata]
 gb|OIT26982.1| isoamylase 1, chloroplastic [Nicotiana attenuata]
          Length = 794

 Score =  591 bits (1523), Expect = 0.0
 Identities = 284/406 (69%), Positives = 323/406 (79%), Gaps = 1/406 (0%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKRGD 326
            ++LLH   I S C PK   + Y   R+N+++F    +    +   VN        +K   
Sbjct: 1    MKLLHCPSI-STCKPKLHSQNYPFSRRNRVDFVSIGRKSRISVAVVNAAADSGRGAKAET 59

Query: 327  TETAVVVE-KPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLR 503
              TAVVVE KP    RRFEV  G P PFGAT  DGGV            TLCLI+LSDL 
Sbjct: 60   AATAVVVEEKPMVERRRFEVLPGKPLPFGATATDGGVNFAVFSSNATSTTLCLITLSDLP 119

Query: 504  EKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPY 683
            +KRVT QI+L PL NKTGDVWHVFLKGDFE+M YGYKFDG FSPE+G +FDSS++++DPY
Sbjct: 120  QKRVTVQISLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFSPEEGHFFDSSQIVLDPY 179

Query: 684  AKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGFT 863
            AKA V RGEYG LGP+ DCWP MA +VPS  D+FDWEGDLPLK PQ DLVIYEMHVRG+T
Sbjct: 180  AKATVSRGEYGVLGPEDDCWPPMAGMVPSAADQFDWEGDLPLKFPQRDLVIYEMHVRGYT 239

Query: 864  RHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWG 1043
             HESS +K+PGTY G+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK+NFWG
Sbjct: 240  NHESSGTKFPGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKLNFWG 299

Query: 1044 YSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGP 1223
            YST+NFFSPM RYSS+G+ N GLGA+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNENGP
Sbjct: 300  YSTVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGP 359

Query: 1224 ILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            I+SFRG+DNSVFYMLAPKGE+YNYSGCGNTFNCNHPIVRQFI+DCL
Sbjct: 360  IVSFRGIDNSVFYMLAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCL 405


>ref|XP_015082414.1| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Solanum pennellii]
 ref|XP_015082415.1| PREDICTED: isoamylase 1, chloroplastic isoform X2 [Solanum pennellii]
          Length = 787

 Score =  587 bits (1514), Expect = 0.0
 Identities = 290/408 (71%), Positives = 324/408 (79%), Gaps = 3/408 (0%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKRGD 326
            ++LLH   I +Y  PK   + +L  R+N       SKP +   I    +V  N A  R  
Sbjct: 1    MELLHSPSIYTY-NPKLNFQNHLFSRRN-------SKPVDFETIRRKSVV--NAAVHRVA 50

Query: 327  TETA---VVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSD 497
             +TA   VVVEK      RFEV SG P PFGAT  DGGV            TLCLI+LSD
Sbjct: 51   AKTAASAVVVEKSTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSD 110

Query: 498  LREKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLID 677
            L EKRVTEQI L PL NKTGDVWHVFLKGDFE+M YGYKFDG F PE+G YFDSS++++D
Sbjct: 111  LPEKRVTEQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLD 170

Query: 678  PYAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRG 857
            PYAKA+V RGEYG LGP+ DCWP MA +VPS +D+FDWEGDLPLK PQ DLVIYEMHVRG
Sbjct: 171  PYAKAIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFPQRDLVIYEMHVRG 230

Query: 858  FTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNF 1037
            FT HESS +KYPGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK NF
Sbjct: 231  FTNHESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNF 290

Query: 1038 WGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNEN 1217
            WGYST+NFFSPM RYSS+G+ N GLGA+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNEN
Sbjct: 291  WGYSTVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNEN 350

Query: 1218 GPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            GPILSFRG+DNSVFY LAPKGE+YNYSGCGNTFNCN+PIVRQFI+DCL
Sbjct: 351  GPILSFRGIDNSVFYTLAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCL 398


>dbj|BAW33106.1| isoamylase [Ipomoea batatas]
          Length = 799

 Score =  587 bits (1514), Expect = 0.0
 Identities = 281/410 (68%), Positives = 325/410 (79%), Gaps = 5/410 (1%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKRGD 326
            ++L+H   I +Y P   +    +R  K  L+FG P   F   G      V+  NA+  G 
Sbjct: 1    MELIHPPSISAYSPKLHSRSSSIR--KTGLSFGFPQNYFAEFGKKARRSVLAVNAAIDGG 58

Query: 327  TET---AVVVEKPP--AMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISL 491
             E+   AVVVEKP    + RRFEVFSGHP PFGAT RDGG+             LCLISL
Sbjct: 59   GESDTKAVVVEKPLPYGLRRRFEVFSGHPAPFGATARDGGINFAVFSSNATSAALCLISL 118

Query: 492  SDLREKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVL 671
            +DL EK+VTEQI L P  NKTGDVWHVFLKGDF++M YGY FDG F+PE+G YFDS+++L
Sbjct: 119  ADLPEKKVTEQIPLDPSINKTGDVWHVFLKGDFDNMLYGYSFDGKFAPEEGHYFDSARIL 178

Query: 672  IDPYAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHV 851
            +DPYAKA+V R E+GALGP+KDCWP MAC++PS  D+FDWEGDLPLK PQ DLVIYEMHV
Sbjct: 179  LDPYAKAIVSRAEFGALGPEKDCWPPMACMLPSA-DKFDWEGDLPLKFPQRDLVIYEMHV 237

Query: 852  RGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKM 1031
            RGFT HESS +++PGTY G+VEKLDHLK LGVNCIELMPCHEFNELE+YSYN VLGDYK+
Sbjct: 238  RGFTNHESSGTEFPGTYRGVVEKLDHLKGLGVNCIELMPCHEFNELEYYSYNPVLGDYKV 297

Query: 1032 NFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGN 1211
            NFWGYST+NFFSPM RYSS+G+   GLGA++EFKYLVREAH+RGIEVIMDVVFNHTAEGN
Sbjct: 298  NFWGYSTVNFFSPMGRYSSAGMHKSGLGAIDEFKYLVREAHRRGIEVIMDVVFNHTAEGN 357

Query: 1212 ENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            ENGP+ SFRG+DNSVFYMLAPKGE+YNYSGCGNTFNCNHP+ RQFILDCL
Sbjct: 358  ENGPMFSFRGVDNSVFYMLAPKGEFYNYSGCGNTFNCNHPVARQFILDCL 407


>ref|XP_016580325.1| PREDICTED: isoamylase 1, chloroplastic-like [Capsicum annuum]
          Length = 518

 Score =  574 bits (1480), Expect = 0.0
 Identities = 282/412 (68%), Positives = 316/412 (76%), Gaps = 7/412 (1%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKRG- 323
            ++LLH   I +Y P K   + +   R+N   F       EN        V+++ A+  G 
Sbjct: 1    MELLHCPSISTYTP-KLCFQNHPFSRRNYAGF-------ENKWRKSRSSVMMDAAADSGR 52

Query: 324  ------DTETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLI 485
                   T T VVVEK     RRFEV SG P PFGAT RDGGV            TLCLI
Sbjct: 53   GGAAAKTTATVVVVEKLTTERRRFEVLSGKPLPFGATARDGGVNFAVFSRNATSATLCLI 112

Query: 486  SLSDLREKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSK 665
            +LSDL EKRVTEQI L PL NKTGDVWHVFLKGDFE+M YGY FDG F PE G YFDSS+
Sbjct: 113  TLSDLPEKRVTEQIFLDPLVNKTGDVWHVFLKGDFENMLYGYNFDGKFCPEGGHYFDSSQ 172

Query: 666  VLIDPYAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEM 845
            +++DPYAKA V RGEYG LGP+ DCWP MA +VPS  D+F WEGDLPLK PQ DLVIYEM
Sbjct: 173  IVLDPYAKATVSRGEYGVLGPEDDCWPPMAGMVPSAPDQFYWEGDLPLKFPQRDLVIYEM 232

Query: 846  HVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDY 1025
            HVRGFT HESS +K PGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYNSVLGDY
Sbjct: 233  HVRGFTNHESSGTKCPGTYLGIVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY 292

Query: 1026 KMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAE 1205
            K NFWGYST+NFFSPM RY+S+G+ N GLGA+NEFKYLV+EAHKRGIEVIMDVVFNHTAE
Sbjct: 293  KFNFWGYSTVNFFSPMGRYASAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAE 352

Query: 1206 GNENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            GNENGPI+SFRG+D  VFYMLAPKGE+YNY+GCGNTFNCNHPIV QFI+DCL
Sbjct: 353  GNENGPIVSFRGIDGGVFYMLAPKGEFYNYAGCGNTFNCNHPIVCQFIVDCL 404


>ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic isoform X2 [Solanum
            lycopersicum]
          Length = 787

 Score =  583 bits (1504), Expect = 0.0
 Identities = 288/408 (70%), Positives = 323/408 (79%), Gaps = 3/408 (0%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKRGD 326
            ++LLH   I +Y  PK   + +L  R+N       SKP +   I    +V  N A  R  
Sbjct: 1    MELLHSPSISTY-KPKLNYQNHLFSRRN-------SKPVDFETIRRKSVV--NAAVHRLA 50

Query: 327  TETA---VVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSD 497
             +TA   VVVEK      RFEV SG P PFGAT  DGGV            TLCLI+LSD
Sbjct: 51   AKTAASAVVVEKSTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSD 110

Query: 498  LREKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLID 677
            L EKRVTEQI L PL NKTGDVWHVFLKGDFE+M YGYKFDG F PE+G YFDSS++++D
Sbjct: 111  LPEKRVTEQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLD 170

Query: 678  PYAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRG 857
            PYAKA+V RGEYG LGP+ DCWP MA +VPS +D+FDWEGDLPLK  Q +LVIYEMHVRG
Sbjct: 171  PYAKAIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFSQRNLVIYEMHVRG 230

Query: 858  FTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNF 1037
            FT HESS +KYPGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK NF
Sbjct: 231  FTNHESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNF 290

Query: 1038 WGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNEN 1217
            WGYST+NFFSPM RYSS+G+ N GLGA+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNEN
Sbjct: 291  WGYSTVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNEN 350

Query: 1218 GPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            GPILSFRG+DNSVFY LAPKGE+YNYSGCGNTFNCN+PIVRQFI+DCL
Sbjct: 351  GPILSFRGIDNSVFYTLAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCL 398


>gb|EPS64233.1| isoamylase isoform 1, partial [Genlisea aurea]
          Length = 774

 Score =  582 bits (1501), Expect = 0.0
 Identities = 276/390 (70%), Positives = 316/390 (81%), Gaps = 9/390 (2%)
 Frame = +3

Query: 219  CRKNKLNFGIPSKPFENTGINVNPMVILNNASKRGDTETAVVVEKPPAMLR--------- 371
            CR+N+   G   +     G    P V+++  ++R   +      +PPA+L          
Sbjct: 4    CRRNEFTVGNRRRFLVLCGEGAKPSVVVDEVNERRSGK-----RRPPALLEKTKIREEPS 58

Query: 372  RFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLREKRVTEQIALSPLTNK 551
            RFEV SG P PFGATPRDGGV             LCLISL DL E +VTE+I L+PLTNK
Sbjct: 59   RFEVSSGQPMPFGATPRDGGVNFAIFSSNASLAALCLISLGDLSEGKVTEEITLNPLTNK 118

Query: 552  TGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYAKAVVRRGEYGALGPD 731
            TGDVWH+FLKGDFE+MAYGY+FDGSFSPEDG YFDSSK+LIDPYAKA+  RGEYGAL PD
Sbjct: 119  TGDVWHIFLKGDFENMAYGYRFDGSFSPEDGLYFDSSKILIDPYAKAIAGRGEYGALAPD 178

Query: 732  KDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGFTRHESSNSKYPGTYLGM 911
             +CWPQMA +VPST DEFDWEGDLPL+LPQ +L+IYEMHVRGFTRHESS +++PGTY G+
Sbjct: 179  GNCWPQMASMVPSTVDEFDWEGDLPLRLPQRELIIYEMHVRGFTRHESSMTEFPGTYSGV 238

Query: 912  VEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGYSTINFFSPMARYSSS 1091
            VEKL++LKK+GVNCIELMPC EFNELE+YSYN VLGDYKMNFWGYS INFFSPMARYSS+
Sbjct: 239  VEKLEYLKKIGVNCIELMPCQEFNELEYYSYNRVLGDYKMNFWGYSPINFFSPMARYSSA 298

Query: 1092 GIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPILSFRGLDNSVFYMLA 1271
            G+ N+GLGAVNEFK+LV+EAHK+GIEVIMDVVFNHTAEGNENGP LSFRG+DNSVFYMLA
Sbjct: 299  GVQNHGLGAVNEFKHLVKEAHKKGIEVIMDVVFNHTAEGNENGPTLSFRGVDNSVFYMLA 358

Query: 1272 PKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            P GEYYNYSGCGNTFNCNHP VRQFI+DCL
Sbjct: 359  PMGEYYNYSGCGNTFNCNHPFVRQFIVDCL 388


>gb|AAY84833.1| isoamylase [Ipomoea batatas]
          Length = 785

 Score =  582 bits (1501), Expect = 0.0
 Identities = 278/409 (67%), Positives = 324/409 (79%), Gaps = 4/409 (0%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNAS--KR 320
            ++LLH   I  Y P   +    +R  K  L+FG P   F        P  +  NA+    
Sbjct: 1    MELLHSPSISQYSPKLHSRSSNIR--KTGLSFGFPHNYFAEFEKKARPSALAVNAAIDGG 58

Query: 321  GDTETAVVVEKPP--AMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLS 494
            G+++TAVVVEKP    +LRRFEV SGHP PFGAT RDGG+             LCLISL+
Sbjct: 59   GESDTAVVVEKPLPYGLLRRFEVLSGHPAPFGATARDGGINFAVFSSNATSAALCLISLA 118

Query: 495  DLREKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLI 674
            DL EK+VTEQI L P  NKTGDVWHVFL+GDF++M YGY FDG F+PE+G YFDS+++L+
Sbjct: 119  DLPEKKVTEQIPLDPSINKTGDVWHVFLQGDFDNMLYGYSFDGKFAPEEGHYFDSARILL 178

Query: 675  DPYAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVR 854
            DPYAKA+V R E+GALGP+KDCWP MAC++P T D+FDWEGDLPLK PQ DLVIYEMHVR
Sbjct: 179  DPYAKAIVSRAEFGALGPEKDCWPPMACMLP-TADKFDWEGDLPLKFPQRDLVIYEMHVR 237

Query: 855  GFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMN 1034
            GFT HESS +++PGTY G+VEKLDHLK LGVNCIELMPCHEFNELE+YSYN VLGDYK+N
Sbjct: 238  GFTNHESSGTEFPGTYRGVVEKLDHLKVLGVNCIELMPCHEFNELEYYSYNPVLGDYKVN 297

Query: 1035 FWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNE 1214
            FWGYST+NFFSPM RYSS+G+   GLGA++EFKYLVREAH+RGIEVIMDVVFNHTAEGNE
Sbjct: 298  FWGYSTVNFFSPMGRYSSAGMHKSGLGAIDEFKYLVREAHRRGIEVIMDVVFNHTAEGNE 357

Query: 1215 NGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            NGP+ SFRG+DNSVFYMLAPKGE+YNYSGCGNTFNCNHP+ RQFIL+ L
Sbjct: 358  NGPMFSFRGVDNSVFYMLAPKGEFYNYSGCGNTFNCNHPVARQFILEFL 406


>gb|PHT75937.1| Isoamylase 1, chloroplastic [Capsicum annuum]
          Length = 797

 Score =  583 bits (1502), Expect = 0.0
 Identities = 285/407 (70%), Positives = 318/407 (78%), Gaps = 2/407 (0%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNA--SKR 320
            ++LLH   I +Y P K   + +   R+N   F       EN        V+++ A  S R
Sbjct: 1    MELLHCPSISTYTP-KLCFQNHPFSRRNYAGF-------ENKWRKSRSSVMMDAAADSGR 52

Query: 321  GDTETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDL 500
            G   T VVVEK     RRFEV SG P PFGAT RDGGV            TLCLI+LSDL
Sbjct: 53   GGAXTVVVVEKLTTERRRFEVLSGKPLPFGATARDGGVNFAVFSRNATSATLCLITLSDL 112

Query: 501  REKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDP 680
             EKRVTEQI L PL NKTGDVWHVFLKGDFE+M YGY FDG F PE+G YFDSS++++DP
Sbjct: 113  PEKRVTEQIFLDPLVNKTGDVWHVFLKGDFENMLYGYNFDGKFCPEEGHYFDSSQIVLDP 172

Query: 681  YAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGF 860
            YAKA V RGEYG LGP+ DCWP MA +VPS  D+F WEGDLPLK PQ DLVIYEMHVRGF
Sbjct: 173  YAKATVSRGEYGVLGPEDDCWPPMAGMVPSAPDQFYWEGDLPLKFPQRDLVIYEMHVRGF 232

Query: 861  TRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFW 1040
            T HESS +K PGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK NFW
Sbjct: 233  TNHESSGTKCPGTYLGIVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFW 292

Query: 1041 GYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENG 1220
            GYST+NFFSPM RY+S+G+ N GLGA+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNENG
Sbjct: 293  GYSTVNFFSPMGRYASAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENG 352

Query: 1221 PILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            PI+SFRG+D  VFYMLAPKGE+YNYSGCGNTFNCNHPIVRQFI+DCL
Sbjct: 353  PIVSFRGIDGGVFYMLAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCL 399


>ref|XP_010323372.1| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Solanum
            lycopersicum]
          Length = 823

 Score =  583 bits (1504), Expect = 0.0
 Identities = 288/408 (70%), Positives = 323/408 (79%), Gaps = 3/408 (0%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKRGD 326
            ++LLH   I +Y  PK   + +L  R+N       SKP +   I    +V  N A  R  
Sbjct: 1    MELLHSPSISTY-KPKLNYQNHLFSRRN-------SKPVDFETIRRKSVV--NAAVHRLA 50

Query: 327  TETA---VVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSD 497
             +TA   VVVEK      RFEV SG P PFGAT  DGGV            TLCLI+LSD
Sbjct: 51   AKTAASAVVVEKSTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSD 110

Query: 498  LREKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLID 677
            L EKRVTEQI L PL NKTGDVWHVFLKGDFE+M YGYKFDG F PE+G YFDSS++++D
Sbjct: 111  LPEKRVTEQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLD 170

Query: 678  PYAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRG 857
            PYAKA+V RGEYG LGP+ DCWP MA +VPS +D+FDWEGDLPLK  Q +LVIYEMHVRG
Sbjct: 171  PYAKAIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFSQRNLVIYEMHVRG 230

Query: 858  FTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNF 1037
            FT HESS +KYPGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK NF
Sbjct: 231  FTNHESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNF 290

Query: 1038 WGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNEN 1217
            WGYST+NFFSPM RYSS+G+ N GLGA+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNEN
Sbjct: 291  WGYSTVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNEN 350

Query: 1218 GPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            GPILSFRG+DNSVFY LAPKGE+YNYSGCGNTFNCN+PIVRQFI+DCL
Sbjct: 351  GPILSFRGIDNSVFYTLAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCL 398


>ref|XP_009588914.1| PREDICTED: isoamylase 1, chloroplastic [Nicotiana tomentosiformis]
          Length = 793

 Score =  582 bits (1499), Expect = 0.0
 Identities = 280/405 (69%), Positives = 317/405 (78%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILNNASKRGD 326
            ++LLH   I +Y  PK   +     R+N ++F    +    +   VN         K   
Sbjct: 1    MKLLHCPSISTY-KPKLHSQDCPFSRRNSVDFVSIGRKSRISVAVVNAAADSGRGVKVET 59

Query: 327  TETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLRE 506
              TAVVVEKP     RFEV  G P PFGAT  DGGV            TLCLI+LSDL +
Sbjct: 60   AATAVVVEKPMIERHRFEVLPGKPLPFGATATDGGVNFAVFSSHATSATLCLITLSDLPQ 119

Query: 507  KRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYA 686
            KRVT QI L PL NKTGDVWHVFLKGDFE+M YGYKFDG FSPE+G +FDSS++++DPYA
Sbjct: 120  KRVTVQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFSPEEGHFFDSSQIVLDPYA 179

Query: 687  KAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGFTR 866
            KA V RGEYG LGP+ DCWP MA +VPS  D+FDWEGDLPLK PQ D+VIYEMHVRG+T 
Sbjct: 180  KATVSRGEYGVLGPEDDCWPPMAGMVPSAADQFDWEGDLPLKFPQRDVVIYEMHVRGYTN 239

Query: 867  HESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGY 1046
            HESS +K+PGTY G+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK NFWGY
Sbjct: 240  HESSGTKFPGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGY 299

Query: 1047 STINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPI 1226
            ST+NFFSPM RYSS+G+ N GLGA+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNENGPI
Sbjct: 300  STVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPI 359

Query: 1227 LSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            +SFRG+DNSVFYMLAPKGE+YNYSGCGNTFNCNHPIVRQFI+DCL
Sbjct: 360  VSFRGVDNSVFYMLAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCL 404


>ref|XP_019188755.1| PREDICTED: isoamylase 1, chloroplastic [Ipomoea nil]
          Length = 800

 Score =  582 bits (1499), Expect = 0.0
 Identities = 282/411 (68%), Positives = 325/411 (79%), Gaps = 6/411 (1%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKPFENTGINVNPMVILN-NASKRG 323
            ++LLH   I  Y P   +    +R  K  LNFG     F   G    P  +L  NA+  G
Sbjct: 1    MELLHSPSISPYSPKLHSRSSNIR--KTGLNFGFLQNYFAEFGKKARPSSVLAVNAAIDG 58

Query: 324  DTE---TAVVVEKPPA--MLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLIS 488
              E   TAVVVEKP    + RRFEVFSGHPTPFGAT R+GG+             L LIS
Sbjct: 59   GGESDTTAVVVEKPLPYELRRRFEVFSGHPTPFGATAREGGINFAVFSSNATSAALYLIS 118

Query: 489  LSDLREKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKV 668
            L+DL +K+VTEQI L P  NKTGDVWHVFLKGDF++M YGY FDG F+PE+G YFDS+++
Sbjct: 119  LADLPQKKVTEQIPLDPSINKTGDVWHVFLKGDFDNMLYGYSFDGKFAPEEGHYFDSTRI 178

Query: 669  LIDPYAKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMH 848
            L+DPYAKA+V R E+GALGP+KDCW  MAC++PS  DEFDWEGDLPLK PQ DLVIYEMH
Sbjct: 179  LLDPYAKAIVSRAEFGALGPEKDCWSPMACMLPSA-DEFDWEGDLPLKFPQRDLVIYEMH 237

Query: 849  VRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYK 1028
            VRGFT+HESS +++PGTY G+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYN VLGDYK
Sbjct: 238  VRGFTKHESSGTEFPGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNPVLGDYK 297

Query: 1029 MNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEG 1208
            +NFWGYST+NFFSPM RYSS+G+   GLGA++EFKYLVREAH+RGIEVIMDVVFNHTAEG
Sbjct: 298  VNFWGYSTVNFFSPMGRYSSAGMHKSGLGAIDEFKYLVREAHRRGIEVIMDVVFNHTAEG 357

Query: 1209 NENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            NENGP+ SFRG+DNSVFYMLAPKGE+YNYSGCGNTFNCNHP+ RQFILDCL
Sbjct: 358  NENGPMFSFRGVDNSVFYMLAPKGEFYNYSGCGNTFNCNHPVARQFILDCL 408


>ref|XP_009771255.1| PREDICTED: isoamylase 1, chloroplastic [Nicotiana sylvestris]
          Length = 792

 Score =  581 bits (1497), Expect = 0.0
 Identities = 284/406 (69%), Positives = 320/406 (78%), Gaps = 1/406 (0%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNF-GIPSKPFENTGINVNPMVILNNASKRG 323
            ++LLH   I +Y  PK   + Y   R+N ++F  I  K   +  + VN        +K  
Sbjct: 1    MKLLHCPLISAY-KPKLHSQNYPFSRRNSVDFVSIGGKSRISVAV-VNAAADSGRGAKAE 58

Query: 324  DTETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLR 503
               TAVVVEKP    RRFEV  G P PFGAT  DGGV            TLCLI+LSDL 
Sbjct: 59   TAATAVVVEKPTMERRRFEVLPGKPLPFGATATDGGVNFAVFSSNATSATLCLITLSDLP 118

Query: 504  EKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPY 683
            +KRVT QI L PL NKTGDVWHVFLKGDFE+M YGYKFDG FSPE G +FDSS++++DPY
Sbjct: 119  QKRVTVQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFSPE-GHFFDSSQIVLDPY 177

Query: 684  AKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGFT 863
            AKA V RGEYG LGP+ DCWP MA +VPS  D+FDWEGDLPLK PQ DLVIYEMHVRG+T
Sbjct: 178  AKATVSRGEYGVLGPEDDCWPLMAGMVPSAADQFDWEGDLPLKFPQRDLVIYEMHVRGYT 237

Query: 864  RHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWG 1043
             HESS +K+PGTY G+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK NFWG
Sbjct: 238  NHESSGTKFPGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWG 297

Query: 1044 YSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGP 1223
            YST+NFFSPM RYSS+G+ N GL A+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNENGP
Sbjct: 298  YSTVNFFSPMGRYSSAGLSNCGLVAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGP 357

Query: 1224 ILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            I+SFRG+DNSVFYMLAPKGE+YNYSGCGNTFNCNHPIVRQFI+DCL
Sbjct: 358  IVSFRGIDNSVFYMLAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCL 403


>ref|XP_016434247.1| PREDICTED: isoamylase 1, chloroplastic-like [Nicotiana tabacum]
          Length = 792

 Score =  580 bits (1496), Expect = 0.0
 Identities = 284/406 (69%), Positives = 320/406 (78%), Gaps = 1/406 (0%)
 Frame = +3

Query: 147  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNF-GIPSKPFENTGINVNPMVILNNASKRG 323
            ++LLH   I +Y  PK   + Y   R+N ++F  I  K   +  + VN        +K  
Sbjct: 1    MKLLHCPLISAY-KPKLHSQNYPFSRRNSVDFVSIGGKSRISVAV-VNAAADSGRGAKAE 58

Query: 324  DTETAVVVEKPPAMLRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLR 503
               TAVVVEKP    RRFEV  G P PFGAT  DGGV            TLCLI+LSDL 
Sbjct: 59   TPATAVVVEKPTMERRRFEVLPGKPLPFGATATDGGVNFAVFSSNATSATLCLITLSDLP 118

Query: 504  EKRVTEQIALSPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPY 683
            +KRVT QI L PL NKTGDVWHVFLKGDFE+M YGYKFDG FSPE G +FDSS++++DPY
Sbjct: 119  QKRVTVQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFSPE-GHFFDSSQIVLDPY 177

Query: 684  AKAVVRRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQCDLVIYEMHVRGFT 863
            AKA V RGEYG LGP+ DCWP MA +VPS  D+FDWEGDLPLK PQ DLVIYEMHVRG+T
Sbjct: 178  AKATVSRGEYGVLGPEDDCWPLMAGMVPSAADQFDWEGDLPLKFPQRDLVIYEMHVRGYT 237

Query: 864  RHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWG 1043
             HESS +K+PGTY G+VEKLDHLK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK NFWG
Sbjct: 238  NHESSGTKFPGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWG 297

Query: 1044 YSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGP 1223
            YST+NFFSPM RYSS+G+ N GL A+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNENGP
Sbjct: 298  YSTVNFFSPMGRYSSAGLSNCGLVAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGP 357

Query: 1224 ILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1361
            I+SFRG+DNSVFYMLAPKGE+YNYSGCGNTFNCNHPIVRQFI+DCL
Sbjct: 358  IVSFRGIDNSVFYMLAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCL 403