BLASTX nr result
ID: Rehmannia28_contig00001168
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia28_contig00001168
(427 letters)
Database: ./nr
84,704,028 sequences; 31,038,470,784 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_011084613.1| PREDICTED: 4-coumarate--CoA ligase 2 [Sesamu... 106 4e-32
ref|XP_012858335.1| PREDICTED: 4-coumarate--CoA ligase 2 [Erythr... 106 5e-32
gb|EPS66025.1| hypothetical protein M569_08750, partial [Genlise... 106 1e-31
gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshu... 105 2e-31
ref|XP_012844063.1| PREDICTED: 4-coumarate--CoA ligase 2-like [E... 104 2e-31
gb|AGW27193.1| 4-coumarate:coenzyme A ligase 3 [Salvia miltiorrh... 103 5e-31
dbj|BAA08366.2| 4-coumarate:CoA ligase [Lithospermum erythrorhizon] 102 3e-30
emb|CAJ43713.1| 4-coumaryl-CoA ligase [Plantago major] 101 6e-30
ref|XP_009351826.1| PREDICTED: 4-coumarate--CoA ligase 2-like [P... 100 1e-29
ref|XP_008234565.1| PREDICTED: 4-coumarate--CoA ligase 2 [Prunus... 100 1e-29
gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium] 100 1e-29
gb|AKZ20937.1| 4-coumarate-CoA ligase, partial [Citrus reticulata] 100 1e-29
gb|AKZ20945.1| 4-coumarate-CoA ligase, partial [Citrus reticulata] 100 1e-29
gb|AKZ20944.1| 4-coumarate-CoA ligase, partial [Citrus sinensis ... 100 1e-29
gb|AKZ20943.1| 4-coumarate-CoA ligase, partial [Citrus junos] 100 1e-29
gb|AKZ20939.1| 4-coumarate-CoA ligase, partial [Citrus limon] gi... 100 1e-29
gb|AKZ20938.1| 4-coumarate-CoA ligase, partial [Citrus jambhiri] 100 1e-29
gb|AKZ20933.1| 4-coumarate-CoA ligase, partial [Citrus daoxianen... 100 1e-29
ref|XP_015964696.1| PREDICTED: 4-coumarate--CoA ligase 2 [Arachi... 100 1e-29
ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis ... 100 1e-29
>ref|XP_011084613.1| PREDICTED: 4-coumarate--CoA ligase 2 [Sesamum indicum]
Length = 569
Score = 106 bits (265), Expect(2) = 4e-32
Identities = 53/55 (96%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+DDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDE AGEVPVA
Sbjct: 451 DDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEAAGEVPVA 505
Score = 58.5 bits (140), Expect(2) = 4e-32
Identities = 30/37 (81%), Positives = 30/37 (81%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAKXXXXXXXSTS 64
RLHKVYFVHAIPKSPAGKILRKDLRAK STS
Sbjct: 533 RLHKVYFVHAIPKSPAGKILRKDLRAKLAAAAPSSTS 569
>ref|XP_012858335.1| PREDICTED: 4-coumarate--CoA ligase 2 [Erythranthe guttata]
gi|604299897|gb|EYU19740.1| hypothetical protein
MIMGU_mgv1a003643mg [Erythranthe guttata]
Length = 572
Score = 106 bits (265), Expect(2) = 5e-32
Identities = 53/55 (96%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+DDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDE AGEVPVA
Sbjct: 455 DDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEAAGEVPVA 509
Score = 58.2 bits (139), Expect(2) = 5e-32
Identities = 27/27 (100%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHKVYFVHAIPKSPAGKILRKDLRAK
Sbjct: 537 RLHKVYFVHAIPKSPAGKILRKDLRAK 563
>gb|EPS66025.1| hypothetical protein M569_08750, partial [Genlisea aurea]
Length = 208
Score = 106 bits (264), Expect(2) = 1e-31
Identities = 52/55 (94%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+DD+FIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDE AGEVPVA
Sbjct: 97 DDDIFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEAAGEVPVA 151
Score = 57.0 bits (136), Expect(2) = 1e-31
Identities = 26/27 (96%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHKVYFVHAIPKSP+GKILRKDLRAK
Sbjct: 180 RLHKVYFVHAIPKSPSGKILRKDLRAK 206
>gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshurica]
Length = 573
Score = 105 bits (263), Expect(2) = 2e-31
Identities = 51/55 (92%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+DD+FIVDRVKELIKFKGFQVPPAELEALL+SHPNIADAAVVPQKDE AGEVPVA
Sbjct: 454 DDDIFIVDRVKELIKFKGFQVPPAELEALLVSHPNIADAAVVPQKDEAAGEVPVA 508
Score = 56.6 bits (135), Expect(2) = 2e-31
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
+LHKVYF+HAIPKSPAGKILRKDLRAK
Sbjct: 536 KLHKVYFIHAIPKSPAGKILRKDLRAK 562
>ref|XP_012844063.1| PREDICTED: 4-coumarate--CoA ligase 2-like [Erythranthe guttata]
gi|604347072|gb|EYU45376.1| hypothetical protein
MIMGU_mgv1a003734mg [Erythranthe guttata]
Length = 567
Score = 104 bits (259), Expect(2) = 2e-31
Identities = 51/55 (92%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+DDVFIVDRVKELIKFKGFQVPPAELEALL+SHP+IADAAVVPQKDE AGEVPVA
Sbjct: 454 DDDVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDEAAGEVPVA 508
Score = 58.2 bits (139), Expect(2) = 2e-31
Identities = 27/27 (100%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHKVYFVHAIPKSPAGKILRKDLRAK
Sbjct: 536 RLHKVYFVHAIPKSPAGKILRKDLRAK 562
>gb|AGW27193.1| 4-coumarate:coenzyme A ligase 3 [Salvia miltiorrhiza]
Length = 569
Score = 103 bits (256), Expect(2) = 5e-31
Identities = 51/55 (92%), Positives = 53/55 (96%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+DDVFIVDRVKELIKFKGFQVPPAELEALLISHP I+DAAVVPQKDE AGEVPVA
Sbjct: 452 DDDVFIVDRVKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEVPVA 506
Score = 58.2 bits (139), Expect(2) = 5e-31
Identities = 27/27 (100%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHKVYFVHAIPKSPAGKILRKDLRAK
Sbjct: 534 RLHKVYFVHAIPKSPAGKILRKDLRAK 560
>dbj|BAA08366.2| 4-coumarate:CoA ligase [Lithospermum erythrorhizon]
Length = 585
Score = 102 bits (255), Expect(2) = 3e-30
Identities = 51/55 (92%), Positives = 53/55 (96%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+D+VFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKD AGEVPVA
Sbjct: 469 DDEVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDAAAGEVPVA 523
Score = 55.8 bits (133), Expect(2) = 3e-30
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHKVYFVH+IPKSP+GKILRKDLRAK
Sbjct: 552 RLHKVYFVHSIPKSPSGKILRKDLRAK 578
>emb|CAJ43713.1| 4-coumaryl-CoA ligase [Plantago major]
Length = 204
Score = 101 bits (252), Expect(2) = 6e-30
Identities = 50/55 (90%), Positives = 53/55 (96%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+DDVFIVDRVKELIKFKGFQVPPAELEALL+SHPNIADAAVVP+ DE AGEVPVA
Sbjct: 88 DDDVFIVDRVKELIKFKGFQVPPAELEALLVSHPNIADAAVVPKIDEAAGEVPVA 142
Score = 56.2 bits (134), Expect(2) = 6e-30
Identities = 26/27 (96%), Positives = 26/27 (96%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLH VYFVHAIPKSPAGKILRKDLRAK
Sbjct: 170 RLHSVYFVHAIPKSPAGKILRKDLRAK 196
>ref|XP_009351826.1| PREDICTED: 4-coumarate--CoA ligase 2-like [Pyrus x bretschneideri]
Length = 606
Score = 100 bits (250), Expect(2) = 1e-29
Identities = 49/55 (89%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+D+VFIVDRVKELIKFKGFQVPPAELE+LLISHP+IADAAVVPQKD+ AGEVPVA
Sbjct: 487 DDEVFIVDRVKELIKFKGFQVPPAELESLLISHPSIADAAVVPQKDDAAGEVPVA 541
Score = 56.2 bits (134), Expect(2) = 1e-29
Identities = 26/27 (96%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHKV+FVHAIPKSPAGKILRKDLRAK
Sbjct: 569 RLHKVHFVHAIPKSPAGKILRKDLRAK 595
>ref|XP_008234565.1| PREDICTED: 4-coumarate--CoA ligase 2 [Prunus mume]
Length = 606
Score = 100 bits (248), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+++VFIVDRVKELIKFKGFQVPPAELE+LL+SHP+IADAAVVPQKDE AGEVPVA
Sbjct: 490 DEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQKDEAAGEVPVA 544
Score = 57.0 bits (136), Expect(2) = 1e-29
Identities = 26/27 (96%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHKVYFVHAIPKSP+GKILRKDLRAK
Sbjct: 572 RLHKVYFVHAIPKSPSGKILRKDLRAK 598
>gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]
Length = 604
Score = 100 bits (248), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+++VFIVDRVKELIKFKGFQVPPAELE+LL+SHP+IADAAVVPQKDE AGEVPVA
Sbjct: 488 DEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQKDEAAGEVPVA 542
Score = 57.0 bits (136), Expect(2) = 1e-29
Identities = 26/27 (96%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHKVYFVHAIPKSP+GKILRKDLRAK
Sbjct: 570 RLHKVYFVHAIPKSPSGKILRKDLRAK 596
>gb|AKZ20937.1| 4-coumarate-CoA ligase, partial [Citrus reticulata]
Length = 561
Score = 100 bits (249), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+D+VFIVDRVKE+IKFKGFQVPPAE+EALL+SHP+IADAAVVPQKDE AGEVPVA
Sbjct: 444 DDEVFIVDRVKEIIKFKGFQVPPAEIEALLLSHPSIADAAVVPQKDEVAGEVPVA 498
Score = 56.6 bits (135), Expect(2) = 1e-29
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHK+YFVHAIPKSP+GKILRKDLRAK
Sbjct: 527 RLHKIYFVHAIPKSPSGKILRKDLRAK 553
>gb|AKZ20945.1| 4-coumarate-CoA ligase, partial [Citrus reticulata]
Length = 560
Score = 100 bits (249), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+D+VFIVDRVKE+IKFKGFQVPPAE+EALL+SHP+IADAAVVPQKDE AGEVPVA
Sbjct: 444 DDEVFIVDRVKEIIKFKGFQVPPAEIEALLLSHPSIADAAVVPQKDEVAGEVPVA 498
Score = 56.6 bits (135), Expect(2) = 1e-29
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHK+YFVHAIPKSP+GKILRKDLRAK
Sbjct: 526 RLHKIYFVHAIPKSPSGKILRKDLRAK 552
>gb|AKZ20944.1| 4-coumarate-CoA ligase, partial [Citrus sinensis x Citrus
trifoliata]
Length = 560
Score = 100 bits (249), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+D+VFIVDRVKE+IKFKGFQVPPAE+EALL+SHP+IADAAVVPQKDE AGEVPVA
Sbjct: 444 DDEVFIVDRVKEIIKFKGFQVPPAEIEALLLSHPSIADAAVVPQKDEVAGEVPVA 498
Score = 56.6 bits (135), Expect(2) = 1e-29
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHK+YFVHAIPKSP+GKILRKDLRAK
Sbjct: 526 RLHKIYFVHAIPKSPSGKILRKDLRAK 552
>gb|AKZ20943.1| 4-coumarate-CoA ligase, partial [Citrus junos]
Length = 560
Score = 100 bits (249), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+D+VFIVDRVKE+IKFKGFQVPPAE+EALL+SHP+IADAAVVPQKDE AGEVPVA
Sbjct: 444 DDEVFIVDRVKEIIKFKGFQVPPAEIEALLLSHPSIADAAVVPQKDEVAGEVPVA 498
Score = 56.6 bits (135), Expect(2) = 1e-29
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHK+YFVHAIPKSP+GKILRKDLRAK
Sbjct: 526 RLHKIYFVHAIPKSPSGKILRKDLRAK 552
>gb|AKZ20939.1| 4-coumarate-CoA ligase, partial [Citrus limon]
gi|916370575|gb|AKZ20940.1| 4-coumarate-CoA ligase,
partial [Citrus limonia]
Length = 560
Score = 100 bits (249), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+D+VFIVDRVKE+IKFKGFQVPPAE+EALL+SHP+IADAAVVPQKDE AGEVPVA
Sbjct: 444 DDEVFIVDRVKEIIKFKGFQVPPAEIEALLLSHPSIADAAVVPQKDEVAGEVPVA 498
Score = 56.6 bits (135), Expect(2) = 1e-29
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHK+YFVHAIPKSP+GKILRKDLRAK
Sbjct: 526 RLHKIYFVHAIPKSPSGKILRKDLRAK 552
>gb|AKZ20938.1| 4-coumarate-CoA ligase, partial [Citrus jambhiri]
Length = 560
Score = 100 bits (249), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+D+VFIVDRVKE+IKFKGFQVPPAE+EALL+SHP+IADAAVVPQKDE AGEVPVA
Sbjct: 444 DDEVFIVDRVKEIIKFKGFQVPPAEIEALLLSHPSIADAAVVPQKDEVAGEVPVA 498
Score = 56.6 bits (135), Expect(2) = 1e-29
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHK+YFVHAIPKSP+GKILRKDLRAK
Sbjct: 526 RLHKIYFVHAIPKSPSGKILRKDLRAK 552
>gb|AKZ20933.1| 4-coumarate-CoA ligase, partial [Citrus daoxianensis]
Length = 560
Score = 100 bits (249), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+D+VFIVDRVKE+IKFKGFQVPPAE+EALL+SHP+IADAAVVPQKDE AGEVPVA
Sbjct: 444 DDEVFIVDRVKEIIKFKGFQVPPAEIEALLLSHPSIADAAVVPQKDEVAGEVPVA 498
Score = 56.6 bits (135), Expect(2) = 1e-29
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHK+YFVHAIPKSP+GKILRKDLRAK
Sbjct: 526 RLHKIYFVHAIPKSPSGKILRKDLRAK 552
>ref|XP_015964696.1| PREDICTED: 4-coumarate--CoA ligase 2 [Arachis duranensis]
Length = 574
Score = 99.8 bits (247), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 53/55 (96%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+D++FIVDRVKELIKFKGFQVPPAELEALL+SHP+IADAAVVPQKD AGEVPVA
Sbjct: 457 DDEIFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVAAGEVPVA 511
Score = 57.0 bits (136), Expect(2) = 1e-29
Identities = 26/27 (96%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHKVYFVHAIPKSP+GKILRKDLRAK
Sbjct: 539 RLHKVYFVHAIPKSPSGKILRKDLRAK 565
>ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinifera]
Length = 570
Score = 99.8 bits (247), Expect(2) = 1e-29
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = -3
Query: 425 EDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEDAGEVPVA 261
+++VFIVDRVKELIKFKGFQVPPAELEALL+SHP+IADAAVVPQKD+ AGEVPVA
Sbjct: 453 DEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDDVAGEVPVA 507
Score = 57.0 bits (136), Expect(2) = 1e-29
Identities = 26/27 (96%), Positives = 27/27 (100%)
Frame = -2
Query: 174 RLHKVYFVHAIPKSPAGKILRKDLRAK 94
RLHKVYFVHAIPKSP+GKILRKDLRAK
Sbjct: 535 RLHKVYFVHAIPKSPSGKILRKDLRAK 561