BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia27_contig00039683
         (1316 letters)

Database: ./nr 
           84,704,028 sequences; 31,038,470,784 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011084469.1| PREDICTED: probable membrane-associated kina...   363   e-121
ref|XP_011076081.1| PREDICTED: probable membrane-associated kina...   328   e-107
ref|XP_012852259.1| PREDICTED: probable membrane-associated kina...   294   7e-94
ref|XP_012858210.1| PREDICTED: probable membrane-associated kina...   227   5e-68
gb|EYU19604.1| hypothetical protein MIMGU_mgv1a013544mg [Erythra...   145   1e-37
ref|XP_011047750.1| PREDICTED: probable membrane-associated kina...   144   2e-35
ref|XP_009587183.1| PREDICTED: probable membrane-associated kina...   143   2e-35
ref|XP_009787280.1| PREDICTED: probable membrane-associated kina...   142   2e-35
ref|XP_006374285.1| hypothetical protein POPTR_0015s05690g [Popu...   143   2e-35
ref|XP_009615505.1| PREDICTED: probable membrane-associated kina...   141   6e-35
ref|XP_004234203.1| PREDICTED: probable membrane-associated kina...   139   2e-34
ref|XP_012090160.1| PREDICTED: probable membrane-associated kina...   139   1e-33
ref|XP_011047751.1| PREDICTED: probable membrane-associated kina...   138   2e-33
ref|XP_015158582.1| PREDICTED: probable membrane-associated kina...   137   2e-33
ref|XP_002318350.1| hypothetical protein POPTR_0012s00970g [Popu...   138   2e-33
ref|XP_002275506.2| PREDICTED: probable membrane-associated kina...   135   2e-32
ref|XP_015068508.1| PREDICTED: probable membrane-associated kina...   133   3e-32
emb|CDP21604.1| unnamed protein product [Coffea canephora]            129   2e-30
ref|XP_009773432.1| PREDICTED: probable membrane-associated kina...   129   4e-30
ref|XP_002513625.1| PREDICTED: probable membrane-associated kina...   129   5e-30

>ref|XP_011084469.1| PREDICTED: probable membrane-associated kinase regulator 2 [Sesamum
           indicum]
          Length = 324

 Score =  363 bits (932), Expect = e-121
 Identities = 206/320 (64%), Positives = 232/320 (72%), Gaps = 10/320 (3%)
 Frame = -1

Query: 941 MEASGFLKFRRNAAPGAXXXXXXXXXD---------SFFDLVFKSPDRVAVRRGDEAAKK 789
           MEA G LKF RNAA GA         D         SFF+LV +SPDRVAV  GDEAA+K
Sbjct: 1   MEAFGILKFWRNAAAGADGRKAAVGMDESEGSDEEDSFFELVLRSPDRVAVTPGDEAARK 60

Query: 788 DFQFIESPRDVLLSKNGLSISNSKDLSPVTLLRSSPKFRVFFM-FRKPSKCEKIESNGEL 612
           DFQFIESPRDV  SKN    SNSK LSPV LLRS+PKF+VF +  RK SK EK ESNG+L
Sbjct: 61  DFQFIESPRDVFFSKN--DFSNSKLLSPVALLRSTPKFKVFMLGLRKSSKWEKTESNGKL 118

Query: 611 KGSPFNLFSKSSKIDENNRFSVKCRVEEMPIASNLARDNSLRSQLLKETSDYDTLSEKSS 432
           KGS  N FSKSS I+E+N +SVKCRVE+MP+AS LAR+NSLRS LLKET+DY+T S KSS
Sbjct: 119 KGSSLNQFSKSSNIEESNFYSVKCRVEKMPVASVLARENSLRSLLLKETADYETSSLKSS 178

Query: 431 RDAVXXXXXXXXXXXXXKVWKRQNEKAKLTDSLTPSSTPVNAPVDLSSMKFSEGSRAGSF 252
            DAV              V K QNEKAK T+SLTP  + + AP  LS  K S GSR GSF
Sbjct: 179 SDAVSRYLKLIKPLYLK-VSKWQNEKAKFTESLTPPPSLLTAPASLSPRKLSGGSRVGSF 237

Query: 251 KIATKRLGKSRSASAAVGLPPPPARRRDDSMLEQHEGIQGAVLYCKKSYNSSSKEISQLF 72
           KI  K LGKSRSASAAV + P P RRRDDS+LEQH+GIQ A+L+CK+SYNSS KE SQLF
Sbjct: 238 KIVAKHLGKSRSASAAVDVAPHPGRRRDDSLLEQHDGIQSAILHCKRSYNSSHKEFSQLF 297

Query: 71  RSSSDPSHEKLGHHGRHSCE 12
           RSSSDPSH+KLGH GR+SCE
Sbjct: 298 RSSSDPSHDKLGHPGRNSCE 317


>ref|XP_011076081.1| PREDICTED: probable membrane-associated kinase regulator 2 [Sesamum
           indicum]
          Length = 327

 Score =  328 bits (840), Expect = e-107
 Identities = 189/327 (57%), Positives = 226/327 (69%), Gaps = 17/327 (5%)
 Frame = -1

Query: 941 MEASGFLKFRRNAAPGAXXXXXXXXXD---------------SFFDLVFKSPDRVAVRRG 807
           MEA G LKF  N A GA                         S FDLVFKSPDR A +R 
Sbjct: 1   MEALGVLKFWGNEAAGADASSECDHLRNAAFGIDDEETDDDDSLFDLVFKSPDRGATKR- 59

Query: 806 DEAAKKDFQFIESPRDVLLSKNGLSISNSKDLSPVTLLRSSPKFRVFFM-FRKPSKCEKI 630
           D A+KKD QF+ESPRDV LSKN    SNSK LSPV +LRS+PKF+VF + F+K SKCEK 
Sbjct: 60  DGASKKDSQFLESPRDVFLSKN--DSSNSKPLSPVAILRSAPKFKVFMLGFKKSSKCEKT 117

Query: 629 ES-NGELKGSPFNLFSKSSKIDENNRFSVKCRVEEMPIASNLARDNSLRSQLLKETSDYD 453
           ES NGELK SP N  SKSSK+ ++NRFSVKCRV ++P+A   ARDNSLRS++LKE+ +Y+
Sbjct: 118 ESSNGELKASPLNQLSKSSKLGQSNRFSVKCRVADVPVAPVFARDNSLRSKMLKESIEYE 177

Query: 452 TLSEKSSRDAVXXXXXXXXXXXXXKVWKRQNEKAKLTDSLTPSSTPVNAPVDLSSMKFSE 273
             S+  S +                 + + ++K K TDS+TPSS+PV APV+LS   FSE
Sbjct: 178 ASSDVLSSEK----SVPKYLKLIKPFYGKASKKTKSTDSVTPSSSPVTAPVNLSPKMFSE 233

Query: 272 GSRAGSFKIATKRLGKSRSASAAVGLPPPPARRRDDSMLEQHEGIQGAVLYCKKSYNSSS 93
           GSR GSFKI T+ LGKSRSASAAVG+ PP  RRRDDS+LEQH+GIQGA+L+CKKSYNSSS
Sbjct: 234 GSRIGSFKIVTRNLGKSRSASAAVGVVPPLVRRRDDSLLEQHDGIQGAILHCKKSYNSSS 293

Query: 92  KEISQLFRSSSDPSHEKLGHHGRHSCE 12
           +E SQLFRS+SDPSHEK    GR+S E
Sbjct: 294 QEYSQLFRSASDPSHEKQRVQGRNSWE 320


>ref|XP_012852259.1| PREDICTED: probable membrane-associated kinase regulator 5
           [Erythranthe guttata] gi|848905666|ref|XP_012852260.1|
           PREDICTED: probable membrane-associated kinase regulator
           5 [Erythranthe guttata] gi|604306112|gb|EYU25169.1|
           hypothetical protein MIMGU_mgv1a010164mg [Erythranthe
           guttata]
          Length = 320

 Score =  294 bits (753), Expect = 7e-94
 Identities = 182/328 (55%), Positives = 216/328 (65%), Gaps = 18/328 (5%)
 Frame = -1

Query: 941 MEASGFLKFRRNAAPGAXXXXXXXXXDS---------------FFDLVFKSPDRVAVRRG 807
           MEA G LKF R+A  G           +               FFDL FKS       RG
Sbjct: 1   MEALGILKFWRSAIAGGDVSSEGDDVLNAASGTDDDEAGKEEAFFDLDFKS------ERG 54

Query: 806 DEAAKKDFQFIESPRDVLLSKNGLSISNSKDLSPVTLLRSSPKFRVFFM-FRKPSKCEKI 630
           D AA KDFQFIESPRDV LSK+    SNSK LSPVT LRS+PKF+VF + F+K SK EK 
Sbjct: 55  D-AASKDFQFIESPRDVFLSKS--DFSNSKSLSPVTTLRSAPKFKVFMLGFKKSSKFEKT 111

Query: 629 E-SNGELKGSPFNLFSKSSKIDENNRFSVKCRVEEMPIASN-LARDNSLRSQLLKETSDY 456
           E SNGE K SP N  SKSSK++++NRFS+KCRV E P AS  L RDNSLRS++LKE  +Y
Sbjct: 112 ELSNGETKASPLNQLSKSSKLEQSNRFSLKCRVAEAPAASPALTRDNSLRSKMLKE--NY 169

Query: 455 DTLSEKSSRDAVXXXXXXXXXXXXXKVWKRQNEKAKLTDSLTPSSTPVNAPVDLSSMKFS 276
           +  S+  S +               KV    + K KLT SLTPSS+PV APV+LS  KFS
Sbjct: 170 EASSDVVSSEKSVPKYLRLIKPFYVKV----SNKTKLTYSLTPSSSPVTAPVNLSPRKFS 225

Query: 275 EGSRAGSFKIATKRLGKSRSASAAVGLPPPPARRRDDSMLEQHEGIQGAVLYCKKSYNSS 96
           EGSR GSFKI T+ L KSRSASAAVG  PP  RRRDDS+LEQH+GIQGA+L+CK+S+NSS
Sbjct: 226 EGSRVGSFKIVTRNLVKSRSASAAVGAAPPSIRRRDDSLLEQHDGIQGAILHCKRSFNSS 285

Query: 95  SKEISQLFRSSSDPSHEKLGHHGRHSCE 12
           S+E  QL R+SS+PS  ++G  GR+SCE
Sbjct: 286 SQEYPQLSRASSEPSQGRMGGQGRNSCE 313


>ref|XP_012858210.1| PREDICTED: probable membrane-associated kinase regulator 2
           [Erythranthe guttata]
          Length = 303

 Score =  227 bits (579), Expect = 5e-68
 Identities = 159/309 (51%), Positives = 176/309 (56%), Gaps = 7/309 (2%)
 Frame = -1

Query: 941 MEASGFLKFRRNAAPGAXXXXXXXXXDSFFDLVFKSPDRVAVRRGDEAAKKDFQFIESPR 762
           MEA G LKF RNAA G           SFFDLV KSPD  A  RG   AKKD +FIESP 
Sbjct: 18  MEALGILKFWRNAADGGGDEA------SFFDLVVKSPDGAAAGRGGGVAKKDLEFIESPT 71

Query: 761 DVLLSKNGLSISNSKDLSPVTLLRSSPKFRVFFM-FRKPSKCEKIESNGELKG-SPFNLF 588
           DV  +KN          SPVT L S+ KF+VF + FRK SKCEK ESNGEL G SP N  
Sbjct: 72  DVFPTKNS---------SPVTPLPSATKFKVFILGFRKLSKCEKTESNGELTGFSPANPI 122

Query: 587 SKSSKIDENNRFSVKCRVEEMPIASNLARDNSLRSQLLKETSDYDTLSEKSS-RDAVXXX 411
            K +K                  AS L RD+SLRS LLKE SD     E S   ++V   
Sbjct: 123 PKCAKT---------------AAASTLGRDDSLRSHLLKEASDCAASPENSPPEESVPKY 167

Query: 410 XXXXXXXXXXKVWKRQNEKAKLTDSLT-PSSTPVNAPVDLSSMKFSEGS-RAGSFKIATK 237
                     KVWKRQNEKAK T S T PSSTP  A    S    S+G  R G FKI TK
Sbjct: 168 LKLFKPLLQSKVWKRQNEKAKSTASSTAPSSTPAKARAHSSPRNSSDGGGRVGGFKIVTK 227

Query: 236 RLGKSRSASAAVGLPPPPARRRDDSMLEQHEGIQGAVLYCKKSYNSS-SKEISQLFRSSS 60
            LGKSRSA + +    PP RRRDD ++EQ +GI+ AVLYCKKSY SS  KE S LFRSS+
Sbjct: 228 HLGKSRSAPSVIA-AAPPIRRRDDLLMEQQDGIRSAVLYCKKSYKSSPGKEFSPLFRSST 286

Query: 59  -DPSHEKLG 36
            D SH KLG
Sbjct: 287 DDASHVKLG 295


>gb|EYU19604.1| hypothetical protein MIMGU_mgv1a013544mg [Erythranthe guttata]
          Length = 217

 Score =  145 bits (366), Expect = 1e-37
 Identities = 112/247 (45%), Positives = 123/247 (49%), Gaps = 4/247 (1%)
 Frame = -1

Query: 941 MEASGFLKFRRNAAPGAXXXXXXXXXDSFFDLVFKSPDRVAVRRGDEAAKKDFQFIESPR 762
           MEA G LKF RNAA G           SFFDLV KSPD  A  RG   AKKD +FIESP 
Sbjct: 1   MEALGILKFWRNAADGGGDEA------SFFDLVVKSPDGAAAGRGGGVAKKDLEFIESPT 54

Query: 761 DVLLSKNGLSISNSKDLSPVTLLRSSPKFRVFFM-FRKPSKCEKIESNGELKGSPFNLFS 585
           DV  +KN          SPVT L S+ KF+VF + FRK SKCEK ESNGEL G     FS
Sbjct: 55  DVFPTKNS---------SPVTPLPSATKFKVFILGFRKLSKCEKTESNGELTG-----FS 100

Query: 584 KSSKIDENNRFSVKCRVEEMPIASNLARDNSLRSQLLKETSDYDTLSEKSS-RDAVXXXX 408
            +                           N + + LLKE SD     E S   ++V    
Sbjct: 101 PA---------------------------NPIPNHLLKEASDCAASPENSPPEESVPKYL 133

Query: 407 XXXXXXXXXKVWKRQNEKAKLTDSLT-PSSTPVNAPVDLSSMKFSEGS-RAGSFKIATKR 234
                    KVWKRQNEKAK T S T PSSTP  A    S    S+G  R G FKI TK 
Sbjct: 134 KLFKPLLQSKVWKRQNEKAKSTASSTAPSSTPAKARAHSSPRNSSDGGGRVGGFKIVTKH 193

Query: 233 LGKSRSA 213
           LGKSRSA
Sbjct: 194 LGKSRSA 200


>ref|XP_011047750.1| PREDICTED: probable membrane-associated kinase regulator 5 isoform
           X1 [Populus euphratica]
          Length = 371

 Score =  144 bits (362), Expect = 2e-35
 Identities = 100/278 (35%), Positives = 156/278 (56%), Gaps = 10/278 (3%)
 Frame = -1

Query: 845 VFKSPDRVAVRRGDEAAKKDFQFIESPR---DVLLSKNGL----SISNSKDLSPVTLLRS 687
           VF S         D+ + K     + P    D LLSK  +     IS+SK  SP++LL+S
Sbjct: 88  VFDSKQAPLHNLADKESHKSQHTFQQPTLSTDHLLSKRKILPIEPISSSKPQSPISLLKS 147

Query: 686 SPKFRVFFMFRKPSKC--EKIESNGELKGSPFNLFSKSSKIDENNR-FSVKCRVEEMPIA 516
           +P+F+V  MF+K      +K E  GE +      F+ +SK  E N+ F+VK ++EE+  A
Sbjct: 148 APRFKVL-MFKKSKSMAPQKTEKTGETE-----YFNANSKKHEGNKSFTVKLKLEEVTNA 201

Query: 515 SNLARDNSLRSQLLKETSDYDTLSEKSSRDAVXXXXXXXXXXXXXKVWKRQNEKAKLTDS 336
           S   + NSLR Q+  ++ D D  S++ S++ +              V  +Q++K + +  
Sbjct: 202 SFFTKQNSLRKQIANDSYDNDA-SKRFSKEMIQKYLKLIKPLYIK-VSNKQSDKMRFSGE 259

Query: 335 LTPSSTPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSRSASAAVGLPPPPARRRDDSML 156
           ++  S P ++   +++ K  +GS     ++ ++ LGKS+SASA  G+ PP   RRDDS+L
Sbjct: 260 VSVGS-PSSSSATVTA-KEKQGSFPAGIRVVSRHLGKSKSASATAGVSPPIGSRRDDSLL 317

Query: 155 EQHEGIQGAVLYCKKSYNSSSKEISQLFRSSSDPSHEK 42
            QH+GIQ A+L+CKKS+NSSS + S + R SSDP HEK
Sbjct: 318 LQHDGIQSAILHCKKSFNSSSSDSSLMSRFSSDPLHEK 355


>ref|XP_009587183.1| PREDICTED: probable membrane-associated kinase regulator 2
           [Nicotiana tomentosiformis]
          Length = 363

 Score =  143 bits (361), Expect = 2e-35
 Identities = 109/328 (33%), Positives = 162/328 (49%), Gaps = 29/328 (8%)
 Frame = -1

Query: 953 CYS-SMEASGFLKFRRNAA-------------------PGAXXXXXXXXXDSFFDLVFKS 834
           C+S SMEA   LKF R AA                   P           DSFFDLVF  
Sbjct: 26  CFSKSMEAFNILKFWRTAAVRDIVSDFDSVNNFVDVHRPLENDEKTNDDEDSFFDLVFTG 85

Query: 833 PDRVAVRRGDEAAKKDFQFIESPRDVLLSKNGLSI-SNSKDLSPVTLLRSSPKFRVFFMF 657
             +      +  +  +    ESP+DV        I SNSK LSP ++LRS+PKFRV F+ 
Sbjct: 86  HPK---EYNNAKSVSETNLPESPKDVFFKPKAFPIDSNSKPLSPNSILRSAPKFRVCFLG 142

Query: 656 RKPSKCEKIESNGELKGSPFNLFSKSSKIDENNRFSVKCRVEEMPIASNLARDN-SLRSQ 480
            + SK EK+  +     SP     K S   ++NRF+ KC+VEE+P +    R N SL+  
Sbjct: 143 FRKSKPEKVGVDDSYAASPKMQDQKLSPKAQSNRFTTKCKVEEVPASCISFRYNKSLKGN 202

Query: 479 LLKETSDYDTLSEKSSRDAVXXXXXXXXXXXXXKVWKRQNEKAKLTDSLT-------PSS 321
            +++  +  ++ + S +D                  KR  +  K++D+++       PS 
Sbjct: 203 QVRKEEEVSSVKDSSKQDMPKYLKLMKPLYSRAS--KRYTDNIKISDNVSRASQLCSPSM 260

Query: 320 TPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSRSASAAVGLPPPPARRRDDSMLEQHEG 141
             +N P   SS +  +GSR     +  K+L KSRS ++  G+   P  RRDDS+LEQ++G
Sbjct: 261 QSLNLPRK-SSEEGKQGSRVAVLGVVRKQLRKSRSMASVPGVSTSPMNRRDDSLLEQNDG 319

Query: 140 IQGAVLYCKKSYNSSSKEISQLFRSSSD 57
           IQ A+L+CK+ Y+S+SK+ S L   S++
Sbjct: 320 IQSAILHCKRCYSSASKDCSVLLSRSAN 347


>ref|XP_009787280.1| PREDICTED: probable membrane-associated kinase regulator 2
           [Nicotiana sylvestris]
          Length = 335

 Score =  142 bits (359), Expect = 2e-35
 Identities = 104/279 (37%), Positives = 153/279 (54%), Gaps = 11/279 (3%)
 Frame = -1

Query: 860 SFFDLVFKSPDR---VAVRRGDEAAKKDFQFIESPRDVLLSKNGLSI-SNSKDLSPVTLL 693
           SFF+LVF  PDR   V        +    +  ESPRDV   +   +I SNSK  SP+ +L
Sbjct: 51  SFFELVFTGPDRRPKVDNNNPKCISVNSPRNPESPRDVFFKQKPFAIDSNSKPQSPIWIL 110

Query: 692 RSSPKFRVFFMFRKPSKCEKIESNGELKGSPFNLFSKSSKIDENNRFSVKCRVEEMPIAS 513
           +S+PKFRVF +  + SK EK+  +     SP        KI ++ RF+ KC+ EE+P+  
Sbjct: 111 KSAPKFRVFLLGFRKSKPEKVGIDDSATASP--------KI-QSKRFTAKCKAEEVPVPM 161

Query: 512 N--LARDNSLRSQLLKETSDYDTLSEKSSR--DAVXXXXXXXXXXXXXKVWKRQNEKAKL 345
           +    RDNSLRS+L KE  +  ++ E   +   A              +  KR  EK  +
Sbjct: 162 SPVFTRDNSLRSKLQKEKEEDLSVDESLKKFIRADVPKYLKLMKPLYARASKRYTEKIGV 221

Query: 344 TDSLTPSSTPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSRSASAA-VGLPPPPARRRD 168
           +   +PS+  + +    +S +  +G+R  +F    K LGKSRSA++   G  P P  RRD
Sbjct: 222 SPLSSPSTQSMCSSPRKASEEGKQGNRVAAFGAVRKHLGKSRSAASTFAGASPSPMNRRD 281

Query: 167 DSML-EQHEGIQGAVLYCKKSYNSSSKEISQLF-RSSSD 57
           DS+L EQ++GIQGA+L+CK+SY+S +K+ S L  RS S+
Sbjct: 282 DSLLQEQNDGIQGAILHCKRSYSSVAKDCSMLLPRSGSE 320


>ref|XP_006374285.1| hypothetical protein POPTR_0015s05690g [Populus trichocarpa]
           gi|550322042|gb|ERP52082.1| hypothetical protein
           POPTR_0015s05690g [Populus trichocarpa]
          Length = 368

 Score =  143 bits (361), Expect = 2e-35
 Identities = 98/250 (39%), Positives = 147/250 (58%), Gaps = 10/250 (4%)
 Frame = -1

Query: 761 DVLLSKNGL----SISNSKDLSPVTLLRSSPKFRVFFMFRKPSKC--EKIESNGELKGSP 600
           D LLSK  +     IS+SK  SP++LL+S+P+F+V  MF+K      +K E  GE +   
Sbjct: 117 DHLLSKRKILPIEPISSSKPQSPISLLKSAPRFKVL-MFKKSKSMASQKTEKTGETES-- 173

Query: 599 FNLFSKSSKIDENNRF-SVKCRVEEMPIASNLARDNSLRSQLLKETSDYDTLSEKSSRDA 423
              F+ +SK  E+N+F +VK ++EE+  AS   + NSLR Q   ++ D DT S++ S++ 
Sbjct: 174 ---FNANSKKHESNKFFTVKLKLEEVTNASFFTKQNSLRKQFANDSYDNDT-SKRFSKEV 229

Query: 422 VXXXXXXXXXXXXXKVWKRQNEKAKLTDSLT---PSSTPVNAPVDLSSMKFSEGSRAGSF 252
           +              V KRQ++K + +  L+   PSS+    P      K  +GS     
Sbjct: 230 IQKYLKLIKPLYIK-VSKRQSDKMRFSGELSVGSPSSSSATVPA-----KEKQGSFPAGI 283

Query: 251 KIATKRLGKSRSASAAVGLPPPPARRRDDSMLEQHEGIQGAVLYCKKSYNSSSKEISQLF 72
           ++ ++ LGKS+SASA  G+ PP   RRDDS+L Q++GIQ A+L+CKKS+N SS++ S + 
Sbjct: 284 RVVSRHLGKSKSASATTGVSPPIGSRRDDSLLLQNDGIQSAILHCKKSFN-SSRDSSLMS 342

Query: 71  RSSSDPSHEK 42
           R SSDP HEK
Sbjct: 343 RFSSDPLHEK 352


>ref|XP_009615505.1| PREDICTED: probable membrane-associated kinase regulator 2
           [Nicotiana tomentosiformis]
          Length = 336

 Score =  141 bits (356), Expect = 6e-35
 Identities = 103/280 (36%), Positives = 153/280 (54%), Gaps = 12/280 (4%)
 Frame = -1

Query: 860 SFFDLVFKSPDRVAVRRGDEAAKK----DFQFIESPRDVLLSKNGLSI-SNSKDLSPVTL 696
           SFFDLVF  PD+      +  +K       +  ESPRDV       +I SNSK  SP+++
Sbjct: 51  SFFDLVFTGPDKWPKVDNNNDSKSVSVNSPRDPESPRDVFFKPKPFAIDSNSKPQSPISI 110

Query: 695 LRSSPKFRVFFMFRKPSKCEKIESNGELKGSPFNLFSKSSKIDENNRFSVKCRVEEMPIA 516
           L+S+P FRVF +  + SK EK+  +     SP        KI  + RF+VKC+ EE+P+ 
Sbjct: 111 LKSAPMFRVFLLGFRKSKPEKVGIDDSPTASP--------KI-RSKRFTVKCKAEEVPVP 161

Query: 515 SN--LARDNSLRSQLLKETSDYDTLSEKSSR--DAVXXXXXXXXXXXXXKVWKRQNEKAK 348
            +    RDNSLRS+L KE  +  ++ E   +   A              +  KR  +K  
Sbjct: 162 MSPVFTRDNSLRSKLQKEKEEDLSIDESLKKFVRADVPKYLKLMKPLYARASKRYTDKIG 221

Query: 347 LTDSLTPSSTPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSRSASAA-VGLPPPPARRR 171
           ++   +PS+  + +    +S +  +G+R  +F    K LGKSRSA++   G  P P  RR
Sbjct: 222 VSPLSSPSTQSMCSSPRKASEEGKQGNRVAAFGAVRKHLGKSRSAASTFAGASPSPMNRR 281

Query: 170 DDSML-EQHEGIQGAVLYCKKSYNSSSKEISQLF-RSSSD 57
           DDS+L EQ++GIQGA+L+CK+SY+S +K+ S L  RS S+
Sbjct: 282 DDSLLQEQNDGIQGAILHCKRSYSSVAKDCSMLLPRSGSE 321


>ref|XP_004234203.1| PREDICTED: probable membrane-associated kinase regulator 2 [Solanum
           lycopersicum]
          Length = 307

 Score =  139 bits (351), Expect = 2e-34
 Identities = 101/273 (36%), Positives = 143/273 (52%), Gaps = 7/273 (2%)
 Frame = -1

Query: 860 SFFDLVFKSPDRVAVRRGDEAAKKDFQFIESPRDVLLSKNGLSISNSKDLSPVTLLRSSP 681
           SFFDLVF  PD       + +        ESPRDV         S SK  SP+++LRS+P
Sbjct: 50  SFFDLVFTGPDGRPKLDNNNSKSVSVNTPESPRDVFFKPKA---SISKPQSPISILRSAP 106

Query: 680 KFRVFFMFRKPSKCEKIESNGELKGSPFNLFSKSSKID-ENNRFSVKCRVEEM--PIASN 510
           KFRVFF+  + +K EK+  +     SP        KI  ++ RFSVKC VEE+  P+   
Sbjct: 107 KFRVFFLGLRKTKLEKVGIDDSSPASP--------KIQTQSKRFSVKCNVEEVRAPVNPV 158

Query: 509 LARDNSLRSQLLKETSDYDTLSEKSSR--DAVXXXXXXXXXXXXXKVWKRQNEKAKLTDS 336
             RDNSLRS+L  E  +  ++ E   +   A              +  KR  +K +++  
Sbjct: 159 FTRDNSLRSKLQSEKEEELSVDESLKKFVRADVPKYLKLMKPLYARASKRYTDKIRVSPL 218

Query: 335 LTPSSTPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSRSASAA-VGLPPPPARRRDDSM 159
            +PS+  +++P      K SE  R  +F    K LGKSRSA++   G  P P  RRDDS+
Sbjct: 219 SSPSAQSMSSP-----RKSSEEKRVATFGSVRKHLGKSRSAASTFAGASPSPLNRRDDSL 273

Query: 158 L-EQHEGIQGAVLYCKKSYNSSSKEISQLFRSS 63
           + EQ++GIQGA+L+CK+ Y+S +K    L R S
Sbjct: 274 VQEQNDGIQGAILHCKRCYSSVAKSSVDLNRWS 306


>ref|XP_012090160.1| PREDICTED: probable membrane-associated kinase regulator 5
           [Jatropha curcas] gi|643706070|gb|KDP22202.1|
           hypothetical protein JCGZ_26033 [Jatropha curcas]
          Length = 368

 Score =  139 bits (349), Expect = 1e-33
 Identities = 99/260 (38%), Positives = 146/260 (56%), Gaps = 7/260 (2%)
 Frame = -1

Query: 770 SPRDVLLSKNGLSISN-SKDLSPVTLLRSSPKFRVFFMFRKPSKCEKIESN--GELKGSP 600
           S  D+L  +  L I   SK  SP++LL+S+P+FRV  MF+K       E+   GE +   
Sbjct: 108 SSTDLLSKRKILPIEPISKPQSPISLLKSAPRFRVL-MFKKSKSMAAKETGKTGETELKG 166

Query: 599 FNLFSKSSKIDENNRFSVKCRVEEMPIASNLARDNSLRSQLLKETSDYDTLSEKSSRDAV 420
             + +  +K  E+  F++K +++E        RDNSLR Q+  ++   D+ S++ S++A+
Sbjct: 167 VFVDAPKNKKQESKLFTIKFKLKEAANVPIFTRDNSLRKQISDDSLSDDS-SKRFSKEAI 225

Query: 419 XXXXXXXXXXXXXKVWKRQNEKAKLTDSLT---PSSTPVNAPVDLSSMKFSEGSRAGSFK 249
                         V K+Q+EK K +  L+   PSS+P   P   S  K  +GS     +
Sbjct: 226 QKYLKLIKPLYIK-VSKKQSEKPKFSGELSAASPSSSPATLPSG-SPKKEKQGSIPAGIR 283

Query: 248 IATKRLGKSRSASAAVGLPPPPARRRDDSMLEQHEGIQGAVLYCKKSYNSS-SKEISQLF 72
           +  K LGKS+SASAA  + PP   RRDDS+L QH+GIQ A+L+CKKS+NSS S++ S L 
Sbjct: 284 VVCKHLGKSKSASAATAVLPPTVTRRDDSLLLQHDGIQSAILHCKKSFNSSTSRDSSLLS 343

Query: 71  RSSSDPSHEKLGHHGRHSCE 12
           R +SDP HEK     R+S E
Sbjct: 344 RFASDPLHEKSMASPRNSHE 363


>ref|XP_011047751.1| PREDICTED: probable membrane-associated kinase regulator 5 isoform
           X2 [Populus euphratica]
          Length = 370

 Score =  138 bits (348), Expect = 2e-33
 Identities = 99/278 (35%), Positives = 156/278 (56%), Gaps = 10/278 (3%)
 Frame = -1

Query: 845 VFKSPDRVAVRRGDEAAKKDFQFIESPR---DVLLSKNGL----SISNSKDLSPVTLLRS 687
           VF S         D+ + K     + P    D LLSK  +     IS+SK  SP++LL+S
Sbjct: 88  VFDSKQAPLHNLADKESHKSQHTFQQPTLSTDHLLSKRKILPIEPISSSKPQSPISLLKS 147

Query: 686 SPKFRVFFMFRKPSKC--EKIESNGELKGSPFNLFSKSSKIDENNR-FSVKCRVEEMPIA 516
           +P+F+V  MF+K      +K E  GE +      F+ +SK  E N+ F+VK ++EE+  A
Sbjct: 148 APRFKVL-MFKKSKSMAPQKTEKTGETE-----YFNANSKKHEGNKSFTVKLKLEEVTNA 201

Query: 515 SNLARDNSLRSQLLKETSDYDTLSEKSSRDAVXXXXXXXXXXXXXKVWKRQNEKAKLTDS 336
           S   + NSLR Q+  ++ D D  S++ S++ +              V  +Q++K + +  
Sbjct: 202 SFFTKQNSLRKQIANDSYDNDA-SKRFSKEMIQKYLKLIKPLYIK-VSNKQSDKMRFSGE 259

Query: 335 LTPSSTPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSRSASAAVGLPPPPARRRDDSML 156
           ++  S P ++   +++ K  +GS     ++ ++ LGKS+SASA  G+ PP   RRDDS+L
Sbjct: 260 VSVGS-PSSSSATVTA-KEKQGSFPAGIRVVSRHLGKSKSASATAGVSPPIGSRRDDSLL 317

Query: 155 EQHEGIQGAVLYCKKSYNSSSKEISQLFRSSSDPSHEK 42
            QH+GIQ A+L+CKKS+N SS++ S + R SSDP HEK
Sbjct: 318 LQHDGIQSAILHCKKSFN-SSRDSSLMSRFSSDPLHEK 354


>ref|XP_015158582.1| PREDICTED: probable membrane-associated kinase regulator 2 [Solanum
           tuberosum]
          Length = 327

 Score =  137 bits (345), Expect = 2e-33
 Identities = 105/275 (38%), Positives = 148/275 (53%), Gaps = 8/275 (2%)
 Frame = -1

Query: 860 SFFDLVFKSPD-RVAVRRGDEAAKKDF-QFIESPRDVLLSKNGLSISN-SKDLSPVTLLR 690
           SFFDLVF  PD R  +    ++   +  +  ESPRDV        I + SK  SP+++L+
Sbjct: 50  SFFDLVFTGPDERPKLDNNSKSVSVNTPREPESPRDVFFKPIAFPIDSISKPQSPISILK 109

Query: 689 SSPKFRVFFMFRKPSKCEKIESNGELKGSPFNLFSKSSKIDENNRFSVKCRVEEMPIASN 510
           S+PKFRVFF+  + +K EK+   G    SP     ++ K+D   RF VKC VEE+P+   
Sbjct: 110 SAPKFRVFFLGLRKTKLEKV---GIDNSSPSGTKFQTQKLDLK-RFPVKCNVEEVPVPVT 165

Query: 509 --LARDNSLRSQLLKETSDYDTLSEKSSRDAVXXXXXXXXXXXXXKVWKRQNEKAKLTDS 336
               RDNSLRS+L  E  +   LS   S                  ++ R +++     S
Sbjct: 166 PVFTRDNSLRSKLQNEKEE--ELSVDESLKKFVRADVPKYLKLMKPLYARASKRYTDKIS 223

Query: 335 LTPSSTPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSRSASA--AVGLPPPPARRRDDS 162
           ++P S+P +A    S  K SE  R  SF    K LGKSRSA++  A G    P  RRD+S
Sbjct: 224 VSPLSSP-SAQSMCSPRKTSEVKRVASFGSVRKHLGKSRSAASTFAAGASSSPLNRRDNS 282

Query: 161 ML-EQHEGIQGAVLYCKKSYNSSSKEISQLFRSSS 60
           +L EQ++GIQGA+L+CK+ Y+S +K    L R+SS
Sbjct: 283 LLQEQNDGIQGAILHCKRCYSSLAKSSENLARASS 317


>ref|XP_002318350.1| hypothetical protein POPTR_0012s00970g [Populus trichocarpa]
           gi|222859023|gb|EEE96570.1| hypothetical protein
           POPTR_0012s00970g [Populus trichocarpa]
          Length = 359

 Score =  138 bits (347), Expect = 2e-33
 Identities = 86/229 (37%), Positives = 133/229 (58%), Gaps = 3/229 (1%)
 Frame = -1

Query: 719 KDLSPVTLLRSSPKFRVFFMFRKPSKCEKIESNGELKGSPFNLFSKSSKIDENNRFSVKC 540
           K  SP++LL+S+P+F++  MF+K       ++  E  G+   L + + K + N  F+VK 
Sbjct: 131 KPQSPISLLKSAPRFKIL-MFKKSKSMASQKT--EKTGATEYLKANNKKHESNKLFTVKF 187

Query: 539 RVEEMPIASNLARDNSLRSQLLKETSDYDTLSEKSSRDAVXXXXXXXXXXXXXKVWKRQN 360
           ++EE+   S   + NSLR Q+  E+ D DT S++ S++ +              V K+ +
Sbjct: 188 KLEEVTNISFFTKQNSLRKQISHESDDNDT-SKRFSKEMIQKYLKLIKPLYIK-VSKKHS 245

Query: 359 EKAKLTDSLT---PSSTPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSRSASAAVGLPP 189
           +K K +  L+   PSS+P   P      K  +GS     ++ ++ LGKS+SASA  G+ P
Sbjct: 246 DKMKFSSELSVGSPSSSPATVPA-----KEKQGSFPSGIRVVSRHLGKSKSASATTGVSP 300

Query: 188 PPARRRDDSMLEQHEGIQGAVLYCKKSYNSSSKEISQLFRSSSDPSHEK 42
           P   RRDDS+L QH+GIQ A+L+CKKS+N SS++ S + R  SDPSHEK
Sbjct: 301 PVVSRRDDSLLLQHDGIQSAILHCKKSFN-SSRDSSSMSRFVSDPSHEK 348


>ref|XP_002275506.2| PREDICTED: probable membrane-associated kinase regulator 2 [Vitis
           vinifera]
          Length = 354

 Score =  135 bits (339), Expect = 2e-32
 Identities = 109/299 (36%), Positives = 154/299 (51%), Gaps = 16/299 (5%)
 Frame = -1

Query: 860 SFFDLVFKSPDRVAVRRGDEAAKKDFQFIESPRDVLLSKNGLSI-SNSKDLSPVTLLRSS 684
           SFFDL    P     +  +E + K  +   S  ++   +  L I S+SK  SP++LLRS+
Sbjct: 68  SFFDLELAVP-----QNKEENSNKSGEG-GSAHELFFKRRILPIESSSKPQSPISLLRSA 121

Query: 683 PKFRVFFMFRKPSKCEKIESNGELKGSPFNLFSKSSKIDENNR-FSVKCRVEEMPIASNL 507
           P+FRVF    + SK EK E   +++ S       S +   N++ F+VK +VEE+P+ S  
Sbjct: 122 PRFRVFMFGLRRSKSEKTEKAEKMEAS-----GVSEQPPRNSKLFTVKFKVEEVPVVSVF 176

Query: 506 ARDNSLRSQLLKETSDYDTLSEKSSRDAVXXXXXXXXXXXXXKVWKRQNEKAKLTDSLTP 327
            R+NS RS     T +  +   K     V             KV K+  EK   T S +P
Sbjct: 177 TRENSSRSFGKHGTEETTSFESKRFSKDVVHKYLKLIKPLYIKVSKKYGEKT--TSSPSP 234

Query: 326 SSTPV-------NAPVDLSSMKFSEGSRAGSF----KIATKRLGKSRSASAAVGLPPP-- 186
           SS+P+         PV   S +     R GS     ++  K LGKSRSASAAV + PP  
Sbjct: 235 SSSPIPGPIFSPKKPVKTQSRREDGEERPGSLPAGLRVVCKHLGKSRSASAAVQMMPPVS 294

Query: 185 -PARRRDDSMLEQHEGIQGAVLYCKKSYNSSSKEISQLFRSSSDPSHEKLGHHGRHSCE 12
             A RRDDS+ +QH+GIQ A+++CK+S NSS+ + S L R +SD SHE+     R S E
Sbjct: 295 FQASRRDDSLAQQHDGIQSAIMHCKRSLNSST-DSSHLSRWTSDSSHEQSLDSSRRSVE 352


>ref|XP_015068508.1| PREDICTED: probable membrane-associated kinase regulator 2 [Solanum
           pennellii]
          Length = 307

 Score =  133 bits (335), Expect = 3e-32
 Identities = 99/273 (36%), Positives = 140/273 (51%), Gaps = 7/273 (2%)
 Frame = -1

Query: 860 SFFDLVFKSPDRVAVRRGDEAAKKDFQFIESPRDVLLSKNGLSISNSKDLSPVTLLRSSP 681
           SFFDLVF  PD       + +        ESPRDV         S SK  SP+++LRS+P
Sbjct: 50  SFFDLVFTGPDGRPKLDNNNSKSVSVNTPESPRDVFFKPKA---SISKPQSPISILRSAP 106

Query: 680 KFRVFFMFRKPSKCEKIESNGELKGSPFNLFSKSSKID-ENNRFSVKCRVEEM--PIASN 510
           K RVFF+  + +K EK+  +     SP        KI  ++ RFSVKC VEE+  P+   
Sbjct: 107 KLRVFFLGLRKTKLEKVGIDDSSPASP--------KIQTQSKRFSVKCNVEEVRAPVNPV 158

Query: 509 LARDNSLRSQLLKETSDYDTLSEKSSR--DAVXXXXXXXXXXXXXKVWKRQNEKAKLTDS 336
             RDNSLRS+L  E  +  ++ E   +   A              +  KR  +K  ++  
Sbjct: 159 FTRDNSLRSKLQSEKEEELSVDESLKKFVRADVPKYLKLMKPLYARASKRYTDKISVSPL 218

Query: 335 LTPSSTPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSRSASAA-VGLPPPPARRRDDSM 159
            +PS+  +++P      K SE  R  +F    K LGKSRSA++   G    P  RRDDS+
Sbjct: 219 SSPSAQSMSSP-----RKSSEEKRVATFGSVRKHLGKSRSAASTFAGASSSPLNRRDDSL 273

Query: 158 L-EQHEGIQGAVLYCKKSYNSSSKEISQLFRSS 63
           + EQ++GIQGA+L+CK+ Y+S +K    L R S
Sbjct: 274 VQEQNDGIQGAILHCKRCYSSVAKSSVDLNRWS 306


>emb|CDP21604.1| unnamed protein product [Coffea canephora]
          Length = 326

 Score =  129 bits (324), Expect = 2e-30
 Identities = 97/284 (34%), Positives = 152/284 (53%), Gaps = 24/284 (8%)
 Frame = -1

Query: 860 SFFDLVFKSPD---RVAVR------------RGD--EAAKKDFQFIESPRDVLLSKNGLS 732
           SFFDLVF +PD   + A+             +GD  E +      +ESP    +    + 
Sbjct: 45  SFFDLVFTAPDCYNKQAIANDANCILPHDSSKGDRREGSIHSLIELESPLKSKVFPVDVV 104

Query: 731 ISNSKDLSPVTLLRSSPKFRVFFMFRKPSKCEKIESN---GELKGSPFNLFSKSSKIDEN 561
             NSK LSP++LL+S+PKFRV  +  + SK +K +SN    E K   F+  ++++  +  
Sbjct: 105 DPNSKPLSPISLLKSAPKFRVLLLGFRKSKSDKPQSNPASSEPKRRQFHKPAQNTNRESK 164

Query: 560 NRFSVKCRVEEMPIASNLARDNSLRSQLLKETSDYDTLSEKSSRDAVXXXXXXXXXXXXX 381
              +VK +VE++PI S  +RDNSLRS+L +E + +D   + SS+                
Sbjct: 165 RLAAVKYKVEQVPIGSLFSRDNSLRSKLQRERT-FDFSIDDSSKPMPKDVPMYLKLIRPL 223

Query: 380 KVW--KRQNEKAKLT-DSLTPSSTPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSRSA- 213
            +   KR ++K +   +  +   +P+++P   S      G   G F+  +K LG+SRSA 
Sbjct: 224 YIRASKRYSDKMRFPWEHQSSRVSPMSSPAGESRRGVDRGG--GGFRAVSKHLGRSRSAA 281

Query: 212 SAAVGLPPPPARRRDDSMLEQHEGIQGAVLYCKKSYNSSSKEIS 81
           S+  G+   PA RRDDS+L  ++GIQ A+L+CK+SYNS +KE S
Sbjct: 282 SSTAGMTASPANRRDDSLLLHNDGIQSAILHCKRSYNSPAKECS 325


>ref|XP_009773432.1| PREDICTED: probable membrane-associated kinase regulator 2
           [Nicotiana sylvestris]
          Length = 351

 Score =  129 bits (323), Expect = 4e-30
 Identities = 100/295 (33%), Positives = 151/295 (51%), Gaps = 27/295 (9%)
 Frame = -1

Query: 860 SFFDLVFKSP----------------DRVAVRRGDEAAKKDFQFIESPRDVLLSKNGLSI 729
           SFFDLVF                   D  +V++G    K      ESP+DV        +
Sbjct: 52  SFFDLVFTGHPKENNNSKSVSETGPLDDSSVKQG----KSKLNLPESPKDVFFKPKAFPV 107

Query: 728 -SNSKDLSPVTLLRSSPKFRVFFMFRKPSKCEKIESNGELKGSP-FNLFSKSSKIDENNR 555
            SNSK LSP  +LRS+PKFRV F+  + SK EK+  N     SP   +  K S   E+ R
Sbjct: 108 DSNSKPLSPNYILRSAPKFRVCFLGFRKSKPEKVGINDSSAASPKIQVGQKLSPKAESTR 167

Query: 554 FSVKCRVEEMPIASNLARDN-SLRSQLLKETSDYDTLSEKSSRDAVXXXXXXXXXXXXXK 378
           F+VKC+VEE+P +    R N S +   +++  D  ++ + S +D                
Sbjct: 168 FTVKCKVEEVPASCISFRYNKSSKGNQVQQEEDDSSVKDSSKQDMPKYLKLMKPLYSRAS 227

Query: 377 VWKRQNEKAKLTDSLT-------PSSTPVNAPVDLSSMKFSEGSRAGSFKIATKRLGKSR 219
             KR  +  K++D+++       PS   ++ P   SS +  +GSR     +  K+L KSR
Sbjct: 228 --KRYTDNIKISDNVSRAGQLCSPSMQSLSLPRK-SSEEGKQGSRVAVLGVVRKQLRKSR 284

Query: 218 SASAAVGLPPPPARRRDDSMLEQHEGIQGAVLYCKKSYNSSSKEIS-QLFRSSSD 57
           S ++ V     P  RRDDS+LEQ++GIQGA+L+CK+ Y+S++K+ S  L RS+++
Sbjct: 285 STASFVA---SPMNRRDDSLLEQNDGIQGAILHCKRCYSSAAKDCSMSLSRSTNE 336


>ref|XP_002513625.1| PREDICTED: probable membrane-associated kinase regulator 5 [Ricinus
           communis] gi|223547533|gb|EEF49028.1| hypothetical
           protein RCOM_1581540 [Ricinus communis]
          Length = 382

 Score =  129 bits (324), Expect = 5e-30
 Identities = 82/236 (34%), Positives = 129/236 (54%), Gaps = 3/236 (1%)
 Frame = -1

Query: 710 SPVTLLRSSPKFRVFFMFRKPSKCEKIESNGELKGSPFNLFSKSSKIDENNRFSVKCRVE 531
           SP++LL+S+P+FRV  MF+K       E+         +  + S+K  E+  F+VK +++
Sbjct: 149 SPISLLKSAPRFRVL-MFKKSRSMAAHETEF------LDTATNSNKKQESKLFTVKFKLK 201

Query: 530 EMPIASNLARDNSLRSQLLKETSDYDTLSEKSSRDAVXXXXXXXXXXXXXKVWKRQNEKA 351
           E+       RDNSLR Q+  ++      S++ S++ +              V K+Q EK 
Sbjct: 202 EVASVPIFTRDNSLRKQISDDSLSDQESSKRFSKEVIQKYLKLIKPLYIK-VSKKQTEKP 260

Query: 350 KLTDSLTPSSTPVNAPVDL---SSMKFSEGSRAGSFKIATKRLGKSRSASAAVGLPPPPA 180
           + +      ++P ++P  +   S  K  +G+     ++  K LGKS+S SA+   PP   
Sbjct: 261 RFSGESLSVASPSSSPATVLCSSPKKEKQGTIPAGIRVVCKHLGKSKSESASTVAPPSTV 320

Query: 179 RRRDDSMLEQHEGIQGAVLYCKKSYNSSSKEISQLFRSSSDPSHEKLGHHGRHSCE 12
            RRDDS+L QH+GIQ A+L+CKKS+NSSS++ S L R +SDP HE+L    R S E
Sbjct: 321 SRRDDSLLLQHDGIQSAILHCKKSFNSSSRDSSLLSRFASDPLHERLVASPRISYE 376