BLASTX nr result
ID: Rehmannia27_contig00022859
seq
BLASTX 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Rehmannia27_contig00022859 (1094 letters) Database: ./nr 84,704,028 sequences; 31,038,470,784 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_012832117.1| PREDICTED: homocysteine S-methyltransferase ... 169 3e-46 ref|XP_012832126.1| PREDICTED: selenocysteine methyltransferase ... 168 3e-46 ref|XP_002300824.2| selenocysteine methyltransferase family prot... 158 6e-42 gb|AFK39355.1| unknown [Medicago truncatula] 154 9e-42 ref|XP_011079886.1| PREDICTED: LOW QUALITY PROTEIN: homocysteine... 157 1e-41 ref|XP_007200361.1| hypothetical protein PRUPE_ppa008204mg [Prun... 157 2e-41 ref|XP_009800629.1| PREDICTED: homocysteine S-methyltransferase ... 155 4e-41 ref|XP_015892056.1| PREDICTED: homocysteine S-methyltransferase ... 155 5e-41 ref|XP_006444023.1| hypothetical protein CICLE_v10020605mg [Citr... 155 5e-41 ref|XP_006479680.1| PREDICTED: selenocysteine methyltransferase ... 155 5e-41 ref|XP_009800628.1| PREDICTED: homocysteine S-methyltransferase ... 155 6e-41 ref|XP_008235379.1| PREDICTED: homocysteine S-methyltransferase ... 155 8e-41 ref|XP_015892055.1| PREDICTED: homocysteine S-methyltransferase ... 155 9e-41 gb|KVI03920.1| hypothetical protein Ccrd_017787 [Cynara carduncu... 156 9e-41 ref|XP_011026126.1| PREDICTED: selenocysteine methyltransferase ... 154 1e-40 ref|XP_010326389.1| PREDICTED: selenocysteine methyltransferase ... 154 1e-40 ref|XP_006444024.1| hypothetical protein CICLE_v10020605mg [Citr... 155 1e-40 ref|XP_008368822.1| PREDICTED: homocysteine S-methyltransferase ... 154 2e-40 ref|XP_013449767.1| homocysteine S-methyltransferase-like protei... 154 2e-40 ref|XP_011026125.1| PREDICTED: homocysteine S-methyltransferase ... 155 3e-40 >ref|XP_012832117.1| PREDICTED: homocysteine S-methyltransferase 2 isoform X1 [Erythranthe guttata] gi|604348397|gb|EYU46552.1| hypothetical protein MIMGU_mgv1a009479mg [Erythranthe guttata] Length = 340 Score = 169 bits (429), Expect = 3e-46 Identities = 86/101 (85%), Positives = 89/101 (88%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R VHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIA EARELYYSRC Sbjct: 54 RAVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIALEARELYYSRCS 113 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY 769 E TDH ++IL QR ILVAASVGSYGAYLADGSEY +Y Sbjct: 114 ESFTDHNPGEKILKQREILVAASVGSYGAYLADGSEYSGDY 154 >ref|XP_012832126.1| PREDICTED: selenocysteine methyltransferase isoform X2 [Erythranthe guttata] Length = 288 Score = 168 bits (425), Expect = 3e-46 Identities = 85/99 (85%), Positives = 88/99 (88%) Frame = -3 Query: 1065 VHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCREG 886 VHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIA EARELYYSRC E Sbjct: 4 VHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIALEARELYYSRCSES 63 Query: 885 LTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY 769 TDH ++IL QR ILVAASVGSYGAYLADGSEY +Y Sbjct: 64 FTDHNPGEKILKQREILVAASVGSYGAYLADGSEYSGDY 102 >ref|XP_002300824.2| selenocysteine methyltransferase family protein [Populus trichocarpa] gi|550344317|gb|EEE80097.2| selenocysteine methyltransferase family protein [Populus trichocarpa] Length = 332 Score = 158 bits (399), Expect = 6e-42 Identities = 83/121 (68%), Positives = 94/121 (77%), Gaps = 2/121 (1%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R VHLDYLEAGADIIITASYQATIQGFE KG+S E+SE LLRKSVEIA EAR++YY RCR Sbjct: 47 RAVHLDYLEAGADIIITASYQATIQGFEAKGFSREDSEALLRKSVEIACEARDIYYGRCR 106 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNV 718 EG D + D R+L RPILVAASVGSYGAYLADGSEY NY I L + +++ + Sbjct: 107 EGSPDGSDDGRVLKHRPILVAASVGSYGAYLADGSEYSGNYGDAITLETLKDFHRRRVQI 166 Query: 717 L 715 L Sbjct: 167 L 167 >gb|AFK39355.1| unknown [Medicago truncatula] Length = 218 Score = 154 bits (389), Expect = 9e-42 Identities = 81/121 (66%), Positives = 93/121 (76%), Gaps = 2/121 (1%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R+VHLDYLE GADII TASYQATIQGF+ KG+S EESEN+LR+SVEIA EAR+LYY RC Sbjct: 54 RQVHLDYLENGADIITTASYQATIQGFKEKGFSNEESENMLRRSVEIACEARDLYYERCA 113 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNV 718 + ADDRIL QRPIL+AASVGSYGAYLADGSEY NY I L + +++ V Sbjct: 114 ACSSGKNADDRILKQRPILIAASVGSYGAYLADGSEYSGNYGDAITLKTLKDFHRRRVQV 173 Query: 717 L 715 L Sbjct: 174 L 174 >ref|XP_011079886.1| PREDICTED: LOW QUALITY PROTEIN: homocysteine S-methyltransferase 2 [Sesamum indicum] Length = 337 Score = 157 bits (398), Expect = 1e-41 Identities = 83/121 (68%), Positives = 93/121 (76%), Gaps = 2/121 (1%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R VHLDYLEAGADIIITASYQATIQGFENKGYSLEESEN A EARELYY RCR Sbjct: 54 RTVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENXXXXKSVXACEARELYYRRCR 113 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNV 718 E T HT+D+R+L QR ILVAASVGSYGAYLADGSEY +Y + LG ++ +++ V Sbjct: 114 EASTGHTSDNRVLKQRKILVAASVGSYGAYLADGSEYSGDYGDAMDLGFLKNFHRRRVKV 173 Query: 717 L 715 L Sbjct: 174 L 174 >ref|XP_007200361.1| hypothetical protein PRUPE_ppa008204mg [Prunus persica] gi|462395761|gb|EMJ01560.1| hypothetical protein PRUPE_ppa008204mg [Prunus persica] Length = 341 Score = 157 bits (397), Expect = 2e-41 Identities = 82/121 (67%), Positives = 96/121 (79%), Gaps = 2/121 (1%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R VHLDYLEAGADIIITASYQATIQGFE KG+S EESE+LLRKSVEIAREAR++YY RC Sbjct: 56 RTVHLDYLEAGADIIITASYQATIQGFEAKGFSTEESEDLLRKSVEIAREARDIYYDRCT 115 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNV 718 EG + + + RIL +R ILVAASVGSYGAYLADGSEY +Y + LG + +++ V Sbjct: 116 EGASGDSGNGRILKRRKILVAASVGSYGAYLADGSEYSGDYGEAMTLGTLKDFHRRRVRV 175 Query: 717 L 715 L Sbjct: 176 L 176 >ref|XP_009800629.1| PREDICTED: homocysteine S-methyltransferase 2-like isoform X2 [Nicotiana sylvestris] Length = 326 Score = 155 bits (393), Expect = 4e-41 Identities = 75/101 (74%), Positives = 90/101 (89%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R VHLDYLEAGADII+++SYQATIQGF+ KGYSLEESE+LL++SVEIA EAR++YY+RC Sbjct: 61 RAVHLDYLEAGADIILSSSYQATIQGFKAKGYSLEESESLLKRSVEIACEARDVYYTRCC 120 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY 769 + L+DH+ D ++L QRPILVAASVGSYGAYLADGSEY Y Sbjct: 121 KSLSDHSTDGKVLKQRPILVAASVGSYGAYLADGSEYSGEY 161 >ref|XP_015892056.1| PREDICTED: homocysteine S-methyltransferase 2 isoform X2 [Ziziphus jujuba] Length = 319 Score = 155 bits (392), Expect = 5e-41 Identities = 82/121 (67%), Positives = 95/121 (78%), Gaps = 2/121 (1%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R+VHLDYLEAGADIIITASYQATIQGF KG+S E+SE+LL+KSVEIA EARELYY RC Sbjct: 58 RQVHLDYLEAGADIIITASYQATIQGFAAKGFSKEQSESLLKKSVEIACEARELYYERCN 117 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNV 718 + +D T D+RIL RPILVAAS+GSYGAYLADGSEY Y I L V + +++ V Sbjct: 118 KSSSDETEDERILKHRPILVAASIGSYGAYLADGSEYSGIYGDAITLEVLKYFHRRRLQV 177 Query: 717 L 715 L Sbjct: 178 L 178 >ref|XP_006444023.1| hypothetical protein CICLE_v10020605mg [Citrus clementina] gi|557546285|gb|ESR57263.1| hypothetical protein CICLE_v10020605mg [Citrus clementina] Length = 333 Score = 155 bits (393), Expect = 5e-41 Identities = 76/101 (75%), Positives = 86/101 (85%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R+VHLDYLEAGADI+I+ASYQATIQGFE KG+S EESE +LR+SVEIAREAR++Y+ RC Sbjct: 95 RQVHLDYLEAGADILISASYQATIQGFEAKGFSREESETMLRRSVEIAREARDMYFERCS 154 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY 769 + D DDRIL RPILVAASVGSYGAYLADGSEY NY Sbjct: 155 KSSCDSVTDDRILKHRPILVAASVGSYGAYLADGSEYSGNY 195 >ref|XP_006479680.1| PREDICTED: selenocysteine methyltransferase [Citrus sinensis] Length = 337 Score = 155 bits (393), Expect = 5e-41 Identities = 76/101 (75%), Positives = 86/101 (85%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R+VHLDYLEAGADI+I+ASYQATIQGFE KG+S EESE +LR+SVEIAREAR++Y+ RC Sbjct: 52 RQVHLDYLEAGADILISASYQATIQGFEAKGFSREESETMLRRSVEIAREARDMYFERCS 111 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY 769 + D DDRIL RPILVAASVGSYGAYLADGSEY NY Sbjct: 112 KSSCDSVTDDRILKHRPILVAASVGSYGAYLADGSEYSGNY 152 >ref|XP_009800628.1| PREDICTED: homocysteine S-methyltransferase 2-like isoform X1 [Nicotiana sylvestris] Length = 342 Score = 155 bits (393), Expect = 6e-41 Identities = 75/101 (74%), Positives = 90/101 (89%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R VHLDYLEAGADII+++SYQATIQGF+ KGYSLEESE+LL++SVEIA EAR++YY+RC Sbjct: 61 RAVHLDYLEAGADIILSSSYQATIQGFKAKGYSLEESESLLKRSVEIACEARDVYYTRCC 120 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY 769 + L+DH+ D ++L QRPILVAASVGSYGAYLADGSEY Y Sbjct: 121 KSLSDHSTDGKVLKQRPILVAASVGSYGAYLADGSEYSGEY 161 >ref|XP_008235379.1| PREDICTED: homocysteine S-methyltransferase 2 [Prunus mume] Length = 341 Score = 155 bits (392), Expect = 8e-41 Identities = 81/121 (66%), Positives = 96/121 (79%), Gaps = 2/121 (1%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R VHLDYLEAGADIIITASYQATIQGF+ KG+S EESE+LLRKSVEIAREAR++YY RC Sbjct: 56 RTVHLDYLEAGADIIITASYQATIQGFKAKGFSTEESEDLLRKSVEIAREARDIYYDRCT 115 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNV 718 EG + + + RIL +R ILVAASVGSYGAYLADGSEY +Y + LG + +++ V Sbjct: 116 EGSSGDSGNGRILKRRKILVAASVGSYGAYLADGSEYSGDYGEAMTLGTLKDFHRRRVRV 175 Query: 717 L 715 L Sbjct: 176 L 176 >ref|XP_015892055.1| PREDICTED: homocysteine S-methyltransferase 2 isoform X1 [Ziziphus jujuba] Length = 343 Score = 155 bits (392), Expect = 9e-41 Identities = 82/121 (67%), Positives = 95/121 (78%), Gaps = 2/121 (1%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R+VHLDYLEAGADIIITASYQATIQGF KG+S E+SE+LL+KSVEIA EARELYY RC Sbjct: 58 RQVHLDYLEAGADIIITASYQATIQGFAAKGFSKEQSESLLKKSVEIACEARELYYERCN 117 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNV 718 + +D T D+RIL RPILVAAS+GSYGAYLADGSEY Y I L V + +++ V Sbjct: 118 KSSSDETEDERILKHRPILVAASIGSYGAYLADGSEYSGIYGDAITLEVLKYFHRRRLQV 177 Query: 717 L 715 L Sbjct: 178 L 178 >gb|KVI03920.1| hypothetical protein Ccrd_017787 [Cynara cardunculus var. scolymus] Length = 375 Score = 156 bits (394), Expect = 9e-41 Identities = 76/98 (77%), Positives = 86/98 (87%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R+VHLDYLEAGADI+ITASYQATIQGFE KGYS E+ E++LR+SVEI REAR+ YY +CR Sbjct: 56 RQVHLDYLEAGADILITASYQATIQGFEAKGYSSEDGESMLRRSVEIXREARDAYYQQCR 115 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYR 778 E +D+T D RIL RPILVAASVGSYGAYLADGSEYR Sbjct: 116 ESSSDYTGDGRILKHRPILVAASVGSYGAYLADGSEYR 153 >ref|XP_011026126.1| PREDICTED: selenocysteine methyltransferase isoform X2 [Populus euphratica] Length = 303 Score = 154 bits (388), Expect = 1e-40 Identities = 81/119 (68%), Positives = 92/119 (77%), Gaps = 2/119 (1%) Frame = -3 Query: 1065 VHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCREG 886 VHLDYLEAGADIIITASYQATIQGFE KG+S +SE LLRKSVEIA EAR++YY RCREG Sbjct: 20 VHLDYLEAGADIIITASYQATIQGFEAKGFSRGDSEALLRKSVEIACEARDIYYGRCREG 79 Query: 885 LTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNVL 715 D + D R+L RPILVAASVGSYGAYLADGSEY NY I L + +++ +L Sbjct: 80 SPDGSDDGRVLKHRPILVAASVGSYGAYLADGSEYSGNYGDAITLETLKDFHRRRVQIL 138 >ref|XP_010326389.1| PREDICTED: selenocysteine methyltransferase isoform X3 [Solanum lycopersicum] Length = 303 Score = 154 bits (388), Expect = 1e-40 Identities = 74/101 (73%), Positives = 88/101 (87%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R VHLDYLEAGADII+++SYQATIQGF+ KGYS+EESE+LL++SVEIA EAR++YY RCR Sbjct: 62 RAVHLDYLEAGADIILSSSYQATIQGFKAKGYSIEESESLLKRSVEIACEARDVYYKRCR 121 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY 769 E +D + D ++L QRPILVAASVGSYGAYLADGSEY Y Sbjct: 122 ESSSDQSTDGKVLKQRPILVAASVGSYGAYLADGSEYSGEY 162 >ref|XP_006444024.1| hypothetical protein CICLE_v10020605mg [Citrus clementina] gi|557546286|gb|ESR57264.1| hypothetical protein CICLE_v10020605mg [Citrus clementina] Length = 380 Score = 155 bits (393), Expect = 1e-40 Identities = 76/101 (75%), Positives = 86/101 (85%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R+VHLDYLEAGADI+I+ASYQATIQGFE KG+S EESE +LR+SVEIAREAR++Y+ RC Sbjct: 95 RQVHLDYLEAGADILISASYQATIQGFEAKGFSREESETMLRRSVEIAREARDMYFERCS 154 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY 769 + D DDRIL RPILVAASVGSYGAYLADGSEY NY Sbjct: 155 KSSCDSVTDDRILKHRPILVAASVGSYGAYLADGSEYSGNY 195 >ref|XP_008368822.1| PREDICTED: homocysteine S-methyltransferase 2-like [Malus domestica] Length = 344 Score = 154 bits (390), Expect = 2e-40 Identities = 80/121 (66%), Positives = 97/121 (80%), Gaps = 2/121 (1%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R+VHLDYLEAGADIIITASYQATIQGF+ KG+S EESE+LLRKSVEIAREAR++YY RC Sbjct: 59 RKVHLDYLEAGADIIITASYQATIQGFKAKGFSTEESESLLRKSVEIAREARDIYYDRCT 118 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNV 718 + + + + RIL +RPILVAASVGSYGAYLADGSEY NY + LG + +++ + Sbjct: 119 QCSSADSGNGRILKRRPILVAASVGSYGAYLADGSEYSGNYGEAMTLGRLKDFHRRRVQI 178 Query: 717 L 715 L Sbjct: 179 L 179 >ref|XP_013449767.1| homocysteine S-methyltransferase-like protein [Medicago truncatula] gi|657379415|gb|KEH23795.1| homocysteine S-methyltransferase-like protein [Medicago truncatula] Length = 340 Score = 154 bits (389), Expect = 2e-40 Identities = 81/121 (66%), Positives = 93/121 (76%), Gaps = 2/121 (1%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R+VHLDYLE GADII TASYQATIQGF+ KG+S EESEN+LR+SVEIA EAR+LYY RC Sbjct: 54 RQVHLDYLENGADIITTASYQATIQGFKEKGFSNEESENMLRRSVEIACEARDLYYERCA 113 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNV 718 + ADDRIL QRPIL+AASVGSYGAYLADGSEY NY I L + +++ V Sbjct: 114 ACSSGKNADDRILKQRPILIAASVGSYGAYLADGSEYSGNYGDAITLKTLKDFHRRRVQV 173 Query: 717 L 715 L Sbjct: 174 L 174 >ref|XP_011026125.1| PREDICTED: homocysteine S-methyltransferase 2 isoform X1 [Populus euphratica] Length = 392 Score = 155 bits (392), Expect = 3e-40 Identities = 82/121 (67%), Positives = 93/121 (76%), Gaps = 2/121 (1%) Frame = -3 Query: 1071 REVHLDYLEAGADIIITASYQATIQGFENKGYSLEESENLLRKSVEIAREARELYYSRCR 892 R VHLDYLEAGADIIITASYQATIQGFE KG+S +SE LLRKSVEIA EAR++YY RCR Sbjct: 107 RAVHLDYLEAGADIIITASYQATIQGFEAKGFSRGDSEALLRKSVEIACEARDIYYGRCR 166 Query: 891 EGLTDHTADDRILNQRPILVAASVGSYGAYLADGSEYRDNY--KIPLGVWASYKKKKQNV 718 EG D + D R+L RPILVAASVGSYGAYLADGSEY NY I L + +++ + Sbjct: 167 EGSPDGSDDGRVLKHRPILVAASVGSYGAYLADGSEYSGNYGDAITLETLKDFHRRRVQI 226 Query: 717 L 715 L Sbjct: 227 L 227