BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia27_contig00006765
         (2013 letters)

Database: ./nr 
           84,704,028 sequences; 31,038,470,784 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011092928.1| PREDICTED: proton-coupled amino acid transpo...   686   0.0  
ref|XP_012833789.1| PREDICTED: proton-coupled amino acid transpo...   674   0.0  
ref|XP_011088718.1| PREDICTED: proton-coupled amino acid transpo...   624   0.0  
gb|EPS65904.1| hypothetical protein M569_08873, partial [Genlise...   583   0.0  
ref|XP_002282927.1| PREDICTED: proton-coupled amino acid transpo...   530   0.0  
ref|XP_010271152.1| PREDICTED: proton-coupled amino acid transpo...   529   0.0  
ref|XP_007043067.1| Transmembrane amino acid transporter family ...   528   e-180
ref|XP_002527144.1| PREDICTED: amino acid transporter ANTL1 [Ric...   526   e-180
ref|XP_002277064.1| PREDICTED: proton-coupled amino acid transpo...   524   e-179
ref|XP_004139971.1| PREDICTED: proton-coupled amino acid transpo...   522   e-178
ref|XP_009780247.1| PREDICTED: proton-coupled amino acid transpo...   521   e-178
emb|CDY59097.1| BnaCnng34220D [Brassica napus]                        521   e-178
emb|CDY54797.1| BnaA06g40910D [Brassica napus]                        521   e-177
ref|XP_013729571.1| PREDICTED: amino acid transporter ANTL2 [Bra...   521   e-177
ref|XP_013731901.1| PREDICTED: amino acid transporter ANTL2 [Bra...   520   e-177
ref|XP_013595228.1| PREDICTED: amino acid transporter ANTL2 [Bra...   520   e-177
ref|XP_009629537.1| PREDICTED: proton-coupled amino acid transpo...   520   e-177
ref|XP_010066350.1| PREDICTED: proton-coupled amino acid transpo...   520   e-177
ref|XP_008448189.1| PREDICTED: proton-coupled amino acid transpo...   518   e-176
ref|XP_009801334.1| PREDICTED: proton-coupled amino acid transpo...   516   e-176

>ref|XP_011092928.1| PREDICTED: proton-coupled amino acid transporter 3-like [Sesamum
            indicum]
          Length = 464

 Score =  686 bits (1769), Expect = 0.0
 Identities = 345/433 (79%), Positives = 366/433 (84%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSLPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLPY 562
            MGF+KQNEA SS NSLRS PREDTPLLS +  PH LSSQPKTFANVFIAIVGAGVLGLPY
Sbjct: 1    MGFEKQNEAGSSTNSLRSFPREDTPLLSSHANPHSLSSQPKTFANVFIAIVGAGVLGLPY 60

Query: 563  TFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGSV 742
            TFMRTGWLTSLI IFFVA  TY GMMLLI  R+KLE SP  AF KI+SFGDLGFAVCGSV
Sbjct: 61   TFMRTGWLTSLIMIFFVAALTYRGMMLLIAARRKLEGSPHCAFSKISSFGDLGFAVCGSV 120

Query: 743  GRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSVYIWSCFP 922
            GRFAVDSM+VLSQAGFCVGYLIFIGN                PKI GVSAKS+YIWSCFP
Sbjct: 121  GRFAVDSMIVLSQAGFCVGYLIFIGNTLSNLFSSPLTLSAASPKIWGVSAKSLYIWSCFP 180

Query: 923  FQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTFFYGL 1102
            FQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDA++F +QMP+VEAFG+LSTFFYGL
Sbjct: 181  FQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDALVFAKQMPLVEAFGTLSTFFYGL 240

Query: 1103 GVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDII 1282
            GVAVYSFEGVGMALPLESEMKD+SKFGKILGLTM+FI                +NTKDII
Sbjct: 241  GVAVYSFEGVGMALPLESEMKDRSKFGKILGLTMIFIAAMYGGFGALGYFAFGDNTKDII 300

Query: 1283 TGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXXX 1462
            T NMGKGLLS++VQ+GLCINLFFTFPLMMNPVYEVFERRFW GKYCLWMRWM        
Sbjct: 301  TANMGKGLLSSLVQVGLCINLFFTFPLMMNPVYEVFERRFWDGKYCLWMRWMLVLAVSLV 360

Query: 1463 XXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGVS 1642
                PNF DFLSLVGSSTCCVLGFVLPALFH+L FKDEM+WRRASADVGIIALGVFLGV+
Sbjct: 361  ALFVPNFADFLSLVGSSTCCVLGFVLPALFHYLAFKDEMNWRRASADVGIIALGVFLGVT 420

Query: 1643 GTWYSLLEIFSVK 1681
            GTWY+LLEIFSVK
Sbjct: 421  GTWYALLEIFSVK 433


>ref|XP_012833789.1| PREDICTED: proton-coupled amino acid transporter 3 [Erythranthe
            guttata] gi|604341095|gb|EYU40480.1| hypothetical protein
            MIMGU_mgv1a006582mg [Erythranthe guttata]
          Length = 438

 Score =  674 bits (1740), Expect = 0.0
 Identities = 342/438 (78%), Positives = 364/438 (83%), Gaps = 4/438 (0%)
 Frame = +2

Query: 383  MGFK--KQNEAESSNNSLRSLPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGL 556
            MGF   K NEA SS N+LR LPREDTPLLS N KPH LSSQPKTFANVFI+IVGAGVLGL
Sbjct: 1    MGFDDDKSNEAGSSTNTLRILPREDTPLLSVNGKPHTLSSQPKTFANVFISIVGAGVLGL 60

Query: 557  PYTFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCG 736
            PYTFMRTGWLTSL+ I FVA  T+HGMMLLI TRKKLEDSP+ AFPKIASFGDLGFAVCG
Sbjct: 61   PYTFMRTGWLTSLLMISFVAALTFHGMMLLIHTRKKLEDSPLAAFPKIASFGDLGFAVCG 120

Query: 737  SVGRFAVDSMVVLSQAGFCVGYLIFIGN--XXXXXXXXXXXXXXXXPKILGVSAKSVYIW 910
            S+GRFAVDSM+VLSQAGFCVGYLIFIGN                  P  LG+SAKS+Y+W
Sbjct: 121  SIGRFAVDSMIVLSQAGFCVGYLIFIGNTLANLSTSSSSSAAAASSPNFLGISAKSLYVW 180

Query: 911  SCFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTF 1090
            SCFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFT+QMP VEAFG+LSTF
Sbjct: 181  SCFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTKQMPAVEAFGTLSTF 240

Query: 1091 FYGLGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENT 1270
            FYGLGVAVYSFEG+GMALPLE+EMKDKSKFGKILGLTM FI                ENT
Sbjct: 241  FYGLGVAVYSFEGIGMALPLETEMKDKSKFGKILGLTMFFIASMYAAFGAMGYFAFGENT 300

Query: 1271 KDIITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXX 1450
            KDIIT NMGKGLLST+VQLGLCINLFFT PLMMNPVYEVFERRF +G+YCLWMRWM    
Sbjct: 301  KDIITANMGKGLLSTVVQLGLCINLFFTLPLMMNPVYEVFERRFSNGRYCLWMRWMLVLG 360

Query: 1451 XXXXXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVF 1630
                    PNF DFLSLVGSSTCCVLGFV PALFH+LTFK+EMSWRRASAD+ I+ LGV 
Sbjct: 361  VSLVALFVPNFADFLSLVGSSTCCVLGFVFPALFHYLTFKEEMSWRRASADISIVVLGVV 420

Query: 1631 LGVSGTWYSLLEIFSVKV 1684
            LGVSGTWYSL+EIFSVKV
Sbjct: 421  LGVSGTWYSLIEIFSVKV 438


>ref|XP_011088718.1| PREDICTED: proton-coupled amino acid transporter 3-like [Sesamum
            indicum]
          Length = 429

 Score =  624 bits (1610), Expect = 0.0
 Identities = 316/423 (74%), Positives = 339/423 (80%), Gaps = 2/423 (0%)
 Frame = +2

Query: 416  SNNSLRSLPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLPYTFMRTGWLTSL 595
            S++S R LPRED+PLL     P ILSSQPKTFANVFI++VGAGVLGLPYTFMRTGWLTSL
Sbjct: 5    SSSSNRPLPREDSPLLPAKGNPQILSSQPKTFANVFISVVGAGVLGLPYTFMRTGWLTSL 64

Query: 596  ITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGSVGRFAVDSMVVL 775
            I IFFVA  TY  MMLLI TRKKLEDSPVG FPKI SFGDLGF VCGS+GRFAVD MVVL
Sbjct: 65   IMIFFVAALTYQCMMLLIHTRKKLEDSPVGGFPKITSFGDLGFVVCGSIGRFAVDFMVVL 124

Query: 776  SQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKI--LGVSAKSVYIWSCFPFQLGLNSIP 949
            SQAGFC+GYLIFIG+                 KI  L VSAKSVYIWSCFPFQLGLNSIP
Sbjct: 125  SQAGFCIGYLIFIGDTLAILFRSSFTSPAAGHKIMELSVSAKSVYIWSCFPFQLGLNSIP 184

Query: 950  SLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTFFYGLGVAVYSFEG 1129
            SLTLLAPLSIFAD+VDLGAMG+VMV+D +IFT+QMP VEAFG+LSTFFYGLGVAVYSFEG
Sbjct: 185  SLTLLAPLSIFADVVDLGAMGLVMVEDFLIFTKQMPAVEAFGTLSTFFYGLGVAVYSFEG 244

Query: 1130 VGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDIITGNMGKGLL 1309
            +GMALPLESEMKDKSKFGKILG+TM+FI                 NT+DIIT NMGKG L
Sbjct: 245  IGMALPLESEMKDKSKFGKILGMTMLFIAVMYGAFGAMGYFAFGGNTRDIITANMGKGTL 304

Query: 1310 STIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXXXXXXXPNFTD 1489
            ST+V+LGLCINLFFTFPLMMNPVYEVFERRFW GKYCLWMRWM            PNF D
Sbjct: 305  STLVKLGLCINLFFTFPLMMNPVYEVFERRFWDGKYCLWMRWMLVLAVSLVALFVPNFAD 364

Query: 1490 FLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGVSGTWYSLLEI 1669
            F SLVGSSTCC LGFVLPA+FH+LTFKDEMSWR AS D  II LG+ L VSGTWYSL EI
Sbjct: 365  FSSLVGSSTCCALGFVLPAMFHYLTFKDEMSWRGASGDFIIIVLGIALAVSGTWYSLSEI 424

Query: 1670 FSV 1678
            F V
Sbjct: 425  FHV 427


>gb|EPS65904.1| hypothetical protein M569_08873, partial [Genlisea aurea]
          Length = 409

 Score =  583 bits (1503), Expect = 0.0
 Identities = 296/414 (71%), Positives = 332/414 (80%), Gaps = 1/414 (0%)
 Frame = +2

Query: 446  EDTPLLSGNDKP-HILSSQPKTFANVFIAIVGAGVLGLPYTFMRTGWLTSLITIFFVALF 622
            EDTPLL+G DKP   LSS+ KTFANVFIAIVGAGVLGLPYTFMRTGW+ SL  +FFVA  
Sbjct: 1    EDTPLLAGIDKPPRKLSSRLKTFANVFIAIVGAGVLGLPYTFMRTGWVASLFMVFFVAAL 60

Query: 623  TYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGSVGRFAVDSMVVLSQAGFCVGY 802
            TYHGMMLL+ TR+K++DSP  +F  IASFGDLGFAVCGS+GRFAVD MV+LSQAGFCVGY
Sbjct: 61   TYHGMMLLVATRRKIQDSPACSFHNIASFGDLGFAVCGSIGRFAVDFMVLLSQAGFCVGY 120

Query: 803  LIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSVYIWSCFPFQLGLNSIPSLTLLAPLSIF 982
            LIFIGN                PKI GVSAK+VYIWSCFPFQLGLN+IPSLTLLAPLSIF
Sbjct: 121  LIFIGNTLGNLSAPNPN-----PKIWGVSAKAVYIWSCFPFQLGLNAIPSLTLLAPLSIF 175

Query: 983  ADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTFFYGLGVAVYSFEGVGMALPLESEM 1162
            AD +DLGAMGVVM++D  IF ++ P VEAFG++STFFYGLGVAVYSFEG+GM LPLESEM
Sbjct: 176  ADALDLGAMGVVMIEDLFIFLKRTPSVEAFGTVSTFFYGLGVAVYSFEGIGMVLPLESEM 235

Query: 1163 KDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDIITGNMGKGLLSTIVQLGLCIN 1342
            KD+SKFGKIL +TM FI                +NTK+IIT NMG GLLST+VQLGLCIN
Sbjct: 236  KDRSKFGKILAITMSFIAAMFGGFGALGYFAYGDNTKEIITANMGTGLLSTLVQLGLCIN 295

Query: 1343 LFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXXXXXXXPNFTDFLSLVGSSTCC 1522
            LFFT PLMMNPVYEV ERRF  G+Y +WMRWM            P+F DFLSLVGSSTCC
Sbjct: 296  LFFTLPLMMNPVYEVCERRFRSGRYSIWMRWMLVLIVVLVALFVPSFADFLSLVGSSTCC 355

Query: 1523 VLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGVSGTWYSLLEIFSVKV 1684
            VLGFVLPALFH+LTFKDEM+ ++A+ADV +I LG+ LGVSGTWYSLLEIFSVKV
Sbjct: 356  VLGFVLPALFHYLTFKDEMNLKQATADVSVIVLGLSLGVSGTWYSLLEIFSVKV 409


>ref|XP_002282927.1| PREDICTED: proton-coupled amino acid transporter 3 [Vitis vinifera]
          Length = 426

 Score =  530 bits (1365), Expect = 0.0
 Identities = 271/427 (63%), Positives = 315/427 (73%)
 Frame = +2

Query: 404  EAESSNNSLRSLPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLPYTFMRTGW 583
            EA SS++ L  +  E+ PLL    K  +LSSQPKTFANVFIA+VGAGVLGLPY F RTGW
Sbjct: 6    EASSSSHGLNPMSPEEAPLLP---KLKLLSSQPKTFANVFIAVVGAGVLGLPYCFKRTGW 62

Query: 584  LTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGSVGRFAVDS 763
            + SL+ +F VA  T+H MMLL+RTR++LE   V  F  IASFGDLGF VCGSVGRFAVD 
Sbjct: 63   VVSLLMLFSVAALTHHCMMLLVRTRRRLES--VMGFTNIASFGDLGFIVCGSVGRFAVDL 120

Query: 764  MVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSVYIWSCFPFQLGLNS 943
            M+VLSQAGFCVGYLIFI N                P+ILG+  K+VYIW C PFQLGLNS
Sbjct: 121  MLVLSQAGFCVGYLIFIANTLANLFNSPTPTNLH-PRILGLMPKTVYIWGCIPFQLGLNS 179

Query: 944  IPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTFFYGLGVAVYSF 1123
            I +LT +APLSIFADIVD+GAMGVVM++D  IF +  P VEA GSLS FFYGLGVAVY+F
Sbjct: 180  ISTLTHMAPLSIFADIVDVGAMGVVMIEDVFIFFKNRPSVEAVGSLSMFFYGLGVAVYAF 239

Query: 1124 EGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDIITGNMGKG 1303
            EGVGM LP+ESE +++ KFGK+L L M  I                E+TKDIIT N+G G
Sbjct: 240  EGVGMVLPIESETQEREKFGKVLALAMASISLMYGGFGALGYFAFGEDTKDIITANLGTG 299

Query: 1304 LLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXXXXXXXPNF 1483
            L+S +VQLGLC+NLFFTFPLMMNPVYEV ERR ++G+YCLW+RW+            PNF
Sbjct: 300  LVSFLVQLGLCVNLFFTFPLMMNPVYEVVERRLYNGRYCLWLRWLLVLTVILVALLVPNF 359

Query: 1484 TDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGVSGTWYSLL 1663
            TDFLSLVGSS CC LGFVLPALFH + FK+EM W   S DVGI+ +GV LGVSGTWYSL+
Sbjct: 360  TDFLSLVGSSVCCGLGFVLPALFHLMVFKEEMGWGGVSLDVGIVVVGVVLGVSGTWYSLV 419

Query: 1664 EIFSVKV 1684
            EIFSVKV
Sbjct: 420  EIFSVKV 426


>ref|XP_010271152.1| PREDICTED: proton-coupled amino acid transporter 3-like [Nelumbo
            nucifera]
          Length = 426

 Score =  529 bits (1363), Expect = 0.0
 Identities = 268/434 (61%), Positives = 313/434 (72%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSLPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLPY 562
            MGF K  EA SS+  LR L REDTPL+    KP  LSS+PKTFANVFIAIVGAGVLGLPY
Sbjct: 1    MGFDK--EASSSSYRLRPLAREDTPLIG---KPQPLSSKPKTFANVFIAIVGAGVLGLPY 55

Query: 563  TFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGSV 742
            TFM+TGW+  ++ I  +A  TYH MMLL+ TR+KLE   +  F KI SFGDLGF VCG +
Sbjct: 56   TFMKTGWVFGILMIATIAFLTYHCMMLLVYTRRKLES--LQGFSKINSFGDLGFTVCGPI 113

Query: 743  GRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSVYIWSCFP 922
            GRFAVD+M+VLSQAGFCV YLIFI N                P+ILG   K  YIW C P
Sbjct: 114  GRFAVDAMIVLSQAGFCVSYLIFIANTLAYVFNSATATNFD-PRILGFKPKVFYIWGCLP 172

Query: 923  FQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTFFYGL 1102
            FQLGLNSI SLT LAPLSIFAD+VDLGAMG+VMV+D  IF +Q P +E+FG LS FFYGL
Sbjct: 173  FQLGLNSISSLTHLAPLSIFADVVDLGAMGIVMVEDVFIFLKQRPALESFGGLSVFFYGL 232

Query: 1103 GVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDII 1282
            GVA+Y+FEG+GM LPLE+E KDK KFGKILGL+M FI                E+TKDII
Sbjct: 233  GVAIYAFEGIGMVLPLETETKDKEKFGKILGLSMAFITLMYGAFGALGYFAFGEDTKDII 292

Query: 1283 TGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXXX 1462
            T N+GKGL+S++VQLGLC+NLFFTFPLMMNPVYE+ ERR   G+YC+W+RW+        
Sbjct: 293  TTNLGKGLVSSLVQLGLCVNLFFTFPLMMNPVYEIVERRLCEGRYCMWLRWVVVLGVTLA 352

Query: 1463 XXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGVS 1642
                PNF DFLSLVGSS CC LGFVLPALFH L FKDE+ W+    D+ I+ LG+  G+S
Sbjct: 353  ALLVPNFADFLSLVGSSVCCALGFVLPALFHLLVFKDEVGWKGLVLDIVIVVLGIIFGIS 412

Query: 1643 GTWYSLLEIFSVKV 1684
            GTW SL+ I + KV
Sbjct: 413  GTWSSLMAILAPKV 426


>ref|XP_007043067.1| Transmembrane amino acid transporter family protein [Theobroma cacao]
            gi|508707002|gb|EOX98898.1| Transmembrane amino acid
            transporter family protein [Theobroma cacao]
          Length = 432

 Score =  528 bits (1361), Expect = e-180
 Identities = 276/438 (63%), Positives = 320/438 (73%), Gaps = 4/438 (0%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRS-LPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLP 559
            MGF+K+  + SS+  LRS LPREDTPL+S  DKP  LSSQPKTFANVFIA+VGAGVLGLP
Sbjct: 1    MGFEKEAISSSSSKKLRSPLPREDTPLIS-KDKP--LSSQPKTFANVFIAVVGAGVLGLP 57

Query: 560  YTFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGS 739
            Y F RTGW+  L+ +FFVA  T + MMLL+ TR+KLE    G F KIASFGDLGFAVCG+
Sbjct: 58   YAFKRTGWIMGLLMLFFVAALTTYCMMLLVYTRRKLESFDNG-FAKIASFGDLGFAVCGT 116

Query: 740  VGRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVS---AKSVYIW 910
            VGR  VD +++LSQAGFCVGYLIFI N                   LG+S   AKS+YIW
Sbjct: 117  VGRCVVDVLIILSQAGFCVGYLIFIANTMANLFNNEISMNSSLS--LGMSSFTAKSLYIW 174

Query: 911  SCFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTF 1090
             CFPFQLGLNSI +LT LAPLSIFAD+VDLGAMGVV+V+D  +  +Q   V AFG LS F
Sbjct: 175  GCFPFQLGLNSIATLTHLAPLSIFADVVDLGAMGVVLVEDLSLILKQRHEVMAFGGLSVF 234

Query: 1091 FYGLGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENT 1270
            FYG+GVAVY+FEG+GM LP+ESEMKD +K GKIL L+M  I                  T
Sbjct: 235  FYGMGVAVYAFEGIGMVLPIESEMKDTAKCGKILALSMGLISLMYGAFGALGYFAFGAET 294

Query: 1271 KDIITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXX 1450
            KDIIT N+G G +ST+VQLGLCINLFFTFPLMMNPVYE+ ERRFW G YCLW+RW+    
Sbjct: 295  KDIITANLGAGWISTLVQLGLCINLFFTFPLMMNPVYEIVERRFWGGNYCLWLRWLLVMI 354

Query: 1451 XXXXXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVF 1630
                    PNF DFLSLVGSS CC LGFVLPA FH L FK+E+ W+  + DVGI+ALG+ 
Sbjct: 355  VSLVALFVPNFADFLSLVGSSVCCGLGFVLPAFFHLLVFKEELGWKGWTLDVGIVALGLV 414

Query: 1631 LGVSGTWYSLLEIFSVKV 1684
            LG+SGTWYSL+EIFSVKV
Sbjct: 415  LGISGTWYSLMEIFSVKV 432


>ref|XP_002527144.1| PREDICTED: amino acid transporter ANTL1 [Ricinus communis]
            gi|223533504|gb|EEF35246.1| amino acid transporter,
            putative [Ricinus communis]
          Length = 433

 Score =  526 bits (1356), Expect = e-180
 Identities = 268/441 (60%), Positives = 316/441 (71%), Gaps = 7/441 (1%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSLPREDTPLLSG-------NDKPHILSSQPKTFANVFIAIVGA 541
            MGF  +  +++    +    +EDTPL++        ND  + LSSQ KTFANVFIAIVGA
Sbjct: 1    MGFGNKEASKTRKPPILPPAKEDTPLITKPNNNNNINDNGNPLSSQSKTFANVFIAIVGA 60

Query: 542  GVLGLPYTFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLG 721
            GVLGLPY F RTGW+ SL+ +F VA  T++ MMLL+ TR+KL+  P G F KI SFGDLG
Sbjct: 61   GVLGLPYAFKRTGWIMSLLMLFSVAALTHYCMMLLVHTRRKLQSLP-GDFSKINSFGDLG 119

Query: 722  FAVCGSVGRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSV 901
            FAVCGSVGRF  D M+VLSQAGFCVGYLIFI N                 +I+G+SAKS 
Sbjct: 120  FAVCGSVGRFVADVMIVLSQAGFCVGYLIFIANTLANLFDMSS-------QIIGLSAKSF 172

Query: 902  YIWSCFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSL 1081
            YIW CFPFQLGLNSI +LT LAPLSIFAD+VDLGAMGVV+V+D +I  +  P V AFG L
Sbjct: 173  YIWGCFPFQLGLNSIATLTHLAPLSIFADLVDLGAMGVVIVEDILIMMKNRPQVNAFGGL 232

Query: 1082 STFFYGLGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXX 1261
            S FFYG+GVAVY+FEG+GM LPLESEMKDK KFG ILGL+M  I                
Sbjct: 233  SVFFYGMGVAVYAFEGIGMVLPLESEMKDKDKFGGILGLSMALISLLYGAFGVLGYFAFG 292

Query: 1262 ENTKDIITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMX 1441
              TKDIIT N+G GL+S++VQLGLCINLFFTFPLMM+PVYE+ ERRFW G+YCLW+RW+ 
Sbjct: 293  NETKDIITANLGAGLISSLVQLGLCINLFFTFPLMMHPVYEIVERRFWGGRYCLWLRWVL 352

Query: 1442 XXXXXXXXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIAL 1621
                       PNF DF+SLVGSS CC LGFVLPALFH L FK+EM W+  S D+ I+ +
Sbjct: 353  VLAVSLVALLVPNFADFMSLVGSSICCGLGFVLPALFHLLVFKEEMDWKGWSVDIAIVTI 412

Query: 1622 GVFLGVSGTWYSLLEIFSVKV 1684
            GV L VSGTWY+L+EIFSVKV
Sbjct: 413  GVVLAVSGTWYALMEIFSVKV 433


>ref|XP_002277064.1| PREDICTED: proton-coupled amino acid transporter 3 [Vitis vinifera]
          Length = 422

 Score =  524 bits (1350), Expect = e-179
 Identities = 271/431 (62%), Positives = 314/431 (72%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSLPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLPY 562
            MGF+K  EA SS+  L S+PREDTPLLS   K   LSSQ KTFANVFIAIVGAGVLGLPY
Sbjct: 1    MGFEK--EASSSSYGLDSVPREDTPLLS---KSRPLSSQTKTFANVFIAIVGAGVLGLPY 55

Query: 563  TFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGSV 742
            TF RTGW+   + +F VA+ TYH MMLL+ TR+KL+   +  F KIASFGDLGFAVCGS+
Sbjct: 56   TFKRTGWVLGSLMLFAVAILTYHCMMLLVHTRRKLDS--LHGFSKIASFGDLGFAVCGSI 113

Query: 743  GRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSVYIWSCFP 922
            GR AVD+M+VLSQAGFC+ YLIFI N                  ILG++ KS YIW CFP
Sbjct: 114  GRVAVDAMIVLSQAGFCISYLIFIANTLAYVSNSSPSNP-----ILGLTPKSFYIWGCFP 168

Query: 923  FQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTFFYGL 1102
            FQLGLNSIP+LT LAPLSIFAD+V++GAMGVVMV+D +IF +Q P + AFG  S FFYGL
Sbjct: 169  FQLGLNSIPTLTHLAPLSIFADVVEIGAMGVVMVEDVLIFLKQRPALRAFGGFSVFFYGL 228

Query: 1103 GVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDII 1282
            GVAVY+FEG+GM LPLESE KDK KFGK+L L+M FI                E TKDII
Sbjct: 229  GVAVYAFEGIGMVLPLESEAKDKDKFGKVLALSMAFISVMYGGFGALGYFAFGEETKDII 288

Query: 1283 TGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXXX 1462
            T N+G+G LS +VQLGLC+NLFFTFPLMMNPVYEV ERRF  G YCLW+RW+        
Sbjct: 289  TTNLGQGPLSIMVQLGLCVNLFFTFPLMMNPVYEVMERRFRDGAYCLWLRWVAVLGVILV 348

Query: 1463 XXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGVS 1642
                PNF DFLSLVGSS CCVL FVLP+LFH + FKD++S +  + DV I+ LG+  GVS
Sbjct: 349  ALMVPNFADFLSLVGSSVCCVLAFVLPSLFHLIVFKDQLSRKGMALDVAILVLGLVFGVS 408

Query: 1643 GTWYSLLEIFS 1675
            GTW SLLEI S
Sbjct: 409  GTWSSLLEIVS 419


>ref|XP_004139971.1| PREDICTED: proton-coupled amino acid transporter 3-like [Cucumis
            sativus] gi|700191452|gb|KGN46656.1| hypothetical protein
            Csa_6G118340 [Cucumis sativus]
          Length = 427

 Score =  522 bits (1344), Expect = e-178
 Identities = 270/435 (62%), Positives = 318/435 (73%), Gaps = 1/435 (0%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRS-LPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLP 559
            MGF +  EA SS+N LRS + REDTPLL     P  LSSQPKTFANVFIAIVGAGVLGLP
Sbjct: 1    MGFDR--EASSSSNRLRSSVAREDTPLLG---TPIPLSSQPKTFANVFIAIVGAGVLGLP 55

Query: 560  YTFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGS 739
            Y F RTGW+ SL+ +F V+  TY+ MMLL+ TR+K+E S +G F KI SFGDLG+ +CGS
Sbjct: 56   YAFKRTGWVMSLLMLFSVSFLTYYCMMLLVYTRRKIE-SLIG-FSKINSFGDLGYTICGS 113

Query: 740  VGRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSVYIWSCF 919
             GR  VD +++LSQ GFCVGYLIFIGN                PKILG+  K VY+W CF
Sbjct: 114  PGRLIVDFLIILSQTGFCVGYLIFIGNTMADVFNSPTVMDLN-PKILGLVPKVVYVWGCF 172

Query: 920  PFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTFFYG 1099
            PFQLGLNSI +LT LAPLSIFADIVDLGAM VVMV+D +I  +Q P VEAFG  S FFYG
Sbjct: 173  PFQLGLNSIQTLTHLAPLSIFADIVDLGAMVVVMVKDVLIIFKQSPSVEAFGGFSVFFYG 232

Query: 1100 LGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDI 1279
            +GVAVY+FEG+GM LPLESE KDK KFG++LGL+M FI                ++TKD+
Sbjct: 233  MGVAVYAFEGIGMVLPLESETKDKEKFGRVLGLSMAFITVLYGAFGTLGYFAFGKDTKDM 292

Query: 1280 ITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXX 1459
            ITGN+G G +ST+V+LGLCINLFFT PLMMNPVYE+ ERRFW G+YCLW+RW+       
Sbjct: 293  ITGNLGSGFISTVVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYCLWLRWLLVFLVSL 352

Query: 1460 XXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGV 1639
                 PNF DFLSLVGS+ CC L FVLPALFHFL FK E+  +    D+GI+ LG+ LGV
Sbjct: 353  VALLVPNFADFLSLVGSAVCCALAFVLPALFHFLVFKQELDIKGWCLDIGILVLGLVLGV 412

Query: 1640 SGTWYSLLEIFSVKV 1684
            SGTW +L+EIFSVKV
Sbjct: 413  SGTWSALVEIFSVKV 427


>ref|XP_009780247.1| PREDICTED: proton-coupled amino acid transporter 3-like [Nicotiana
            sylvestris]
          Length = 430

 Score =  521 bits (1342), Expect = e-178
 Identities = 264/423 (62%), Positives = 314/423 (74%), Gaps = 1/423 (0%)
 Frame = +2

Query: 410  ESSNNSLRSLPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLPYTFMRTGWLT 589
            ESS   L  LP+E+TP+L  +      S  PKTFAN FIAIVGAGVLGLPYTFM+TGW+T
Sbjct: 13   ESSKEYL--LPKEETPILENSK-----SCLPKTFANFFIAIVGAGVLGLPYTFMKTGWIT 65

Query: 590  SLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGSVGRFAVDSMV 769
             L+T+F VA  TYHGMMLLI TRK+L+ S +G   KI+SFGDLGF VCG +GRFAVD M+
Sbjct: 66   GLVTLFSVAALTYHGMMLLIHTRKRLDLSLIGD-SKISSFGDLGFVVCGPIGRFAVDVMI 124

Query: 770  VLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSVYIWSCFPFQLGLNSIP 949
            VL+Q GFC+GYLIFIGN                P  L  S KS+YIW CFPFQLGL SIP
Sbjct: 125  VLAQVGFCIGYLIFIGNTLSHLLSLSKGFGIGIP--LFSSTKSLYIWGCFPFQLGLTSIP 182

Query: 950  SLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPV-VEAFGSLSTFFYGLGVAVYSFE 1126
            SLTLLAPLSIFAD+  +GAMGVVMV+D + FT Q P  + AFG++STFFYGLGVA+YSFE
Sbjct: 183  SLTLLAPLSIFADVAQIGAMGVVMVEDVMTFTNQWPSDIHAFGTVSTFFYGLGVALYSFE 242

Query: 1127 GVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDIITGNMGKGL 1306
            GVGMA+PLE+EMKDKSK+GKIL L+M  I                 +TKDI+T NMGKG+
Sbjct: 243  GVGMAIPLEAEMKDKSKYGKILALSMFLIALIYGTFGVMGYFAFGSSTKDIVTSNMGKGV 302

Query: 1307 LSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXXXXXXXPNFT 1486
            LST+V++GLCINLFFTFPLM+NP +EVFERRF  G YC+WMRW+            PNF 
Sbjct: 303  LSTLVKVGLCINLFFTFPLMLNPAFEVFERRFCDGNYCVWMRWILVLGVTLVALLVPNFA 362

Query: 1487 DFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGVSGTWYSLLE 1666
            DF+SLVGSSTCC LGF+LPALFH+  FK++M  + A  D+G+IALGVF GVSGTWYSL+E
Sbjct: 363  DFMSLVGSSTCCTLGFILPALFHYQVFKEDMDRKGAFFDLGLIALGVFFGVSGTWYSLVE 422

Query: 1667 IFS 1675
            +F+
Sbjct: 423  MFA 425


>emb|CDY59097.1| BnaCnng34220D [Brassica napus]
          Length = 443

 Score =  521 bits (1343), Expect = e-178
 Identities = 272/444 (61%), Positives = 315/444 (70%), Gaps = 10/444 (2%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSL----PREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVL 550
            MGF K+  + SS++S  SL    PREDTPLL G   P  LSSQ KTFANVFIA+VGAGVL
Sbjct: 1    MGFAKEASSSSSSSSSYSLKMPPPREDTPLLLGKSPPP-LSSQFKTFANVFIAVVGAGVL 59

Query: 551  GLPYTFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAV 730
            GLPY F RTGWL  ++ +  V++ T+H MMLL+ TR++L+    G F KI SFGDLGFAV
Sbjct: 60   GLPYAFKRTGWLMGVLLLLSVSVLTHHCMMLLVHTRRRLDSFNGGGFSKIGSFGDLGFAV 119

Query: 731  CGSVGRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXP------KILGVSA 892
            CGS+GR  VD  ++LSQAGFCVGYLIFIG                        + LG+S+
Sbjct: 120  CGSLGRLVVDLFIILSQAGFCVGYLIFIGTTLANIFDPDSPTSLRHQITRLGSEFLGLSS 179

Query: 893  KSVYIWSCFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAF 1072
            KS+YIW CFPFQLGLNSI +LT LAPLSIFAD+VDL AM VV+V+D+II  +Q P V AF
Sbjct: 180  KSLYIWGCFPFQLGLNSIKTLTHLAPLSIFADVVDLAAMAVVVVEDSIIILKQRPDVVAF 239

Query: 1073 GSLSTFFYGLGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXX 1252
            G  S F YG+GVAVYSFEGVGM LPLESEMKDK KFGK+L L M FI             
Sbjct: 240  GGFSLFLYGMGVAVYSFEGVGMVLPLESEMKDKDKFGKVLALGMGFISLIYIAFGFLGYL 299

Query: 1253 XXXENTKDIITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMR 1432
               E+T DIITGN+G GL+STIVQLGLCINLFFTFPLMMNPV+E+ ERRF  G Y  W+R
Sbjct: 300  AFGEDTMDIITGNLGAGLISTIVQLGLCINLFFTFPLMMNPVFEIVERRFSGGMYSAWLR 359

Query: 1433 WMXXXXXXXXXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGI 1612
            W+            PNFTDFLSLVGSSTCC+LGFVLPALFH L FK+EM W++ S D+ I
Sbjct: 360  WLLVLAVTLVALFVPNFTDFLSLVGSSTCCILGFVLPALFHLLVFKEEMGWKQWSLDMAI 419

Query: 1613 IALGVFLGVSGTWYSLLEIFSVKV 1684
            + LGV L VSGTW SL EIFSVKV
Sbjct: 420  VGLGVVLAVSGTWSSLSEIFSVKV 443


>emb|CDY54797.1| BnaA06g40910D [Brassica napus]
          Length = 445

 Score =  521 bits (1342), Expect = e-177
 Identities = 274/447 (61%), Positives = 317/447 (70%), Gaps = 13/447 (2%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSL----PREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVL 550
            MGF K+  + SS++S  SL    PREDTPLL G   P  LSSQ KTFANVFIA+VGAGVL
Sbjct: 1    MGFAKEASSSSSSSSSYSLKMPPPREDTPLLLGKSPP--LSSQFKTFANVFIAVVGAGVL 58

Query: 551  GLPYTFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLED-SPVGAFPKIASFGDLGFA 727
            GLPY F RTGWL  ++ +  V++ T+H MMLL+ TR+KL+  +  G F KI SFGDLGFA
Sbjct: 59   GLPYAFKRTGWLMGVLLLLSVSVLTHHCMMLLVHTRRKLDSFNGGGGFSKIGSFGDLGFA 118

Query: 728  VCGSVGRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXX--------PKILG 883
            VCGS+GR  VD  ++LSQAGFCVGYLIFIG                          + LG
Sbjct: 119  VCGSLGRLVVDLFIILSQAGFCVGYLIFIGTTLANIFDPDSPTSLSLRHQITTLGSEFLG 178

Query: 884  VSAKSVYIWSCFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVV 1063
            VS+KS+YIW CFPFQLGLNSI +LT LAPLSIFAD+VDL AM VV+V+D++I  +Q P V
Sbjct: 179  VSSKSLYIWGCFPFQLGLNSIKTLTHLAPLSIFADVVDLAAMAVVVVEDSMIILKQRPDV 238

Query: 1064 EAFGSLSTFFYGLGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXX 1243
             AFG  S FFYG+GVAVYSFEGVGM LPLESEMKDK KFGK+L L M FI          
Sbjct: 239  VAFGGFSLFFYGMGVAVYSFEGVGMVLPLESEMKDKDKFGKVLALGMGFISFIYIAFGFL 298

Query: 1244 XXXXXXENTKDIITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCL 1423
                  E+T DIITGN+G GL+STIVQLGLCINLFFTFPLMMNPV+E+ ERRF  G Y  
Sbjct: 299  GYLAFGEDTMDIITGNLGAGLISTIVQLGLCINLFFTFPLMMNPVFEIVERRFSGGMYSA 358

Query: 1424 WMRWMXXXXXXXXXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASAD 1603
            W+RW+            PNFTDFLSLVGSSTCC+LGFVLPALFH L FK+EM W++ S D
Sbjct: 359  WLRWLLVLAVTLVALFVPNFTDFLSLVGSSTCCILGFVLPALFHLLVFKEEMGWKQWSLD 418

Query: 1604 VGIIALGVFLGVSGTWYSLLEIFSVKV 1684
            + I+ LGV L VSGTW SL EIFSVKV
Sbjct: 419  MAIVGLGVVLAVSGTWSSLSEIFSVKV 445


>ref|XP_013729571.1| PREDICTED: amino acid transporter ANTL2 [Brassica napus]
          Length = 443

 Score =  521 bits (1341), Expect = e-177
 Identities = 271/444 (61%), Positives = 315/444 (70%), Gaps = 10/444 (2%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSL----PREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVL 550
            MGF K+  + SS++S  SL    PREDTPLL G   P  LSSQ KTFANVFIA+VGAGVL
Sbjct: 1    MGFAKEASSSSSSSSSYSLKMPPPREDTPLLLGKSPPP-LSSQFKTFANVFIAVVGAGVL 59

Query: 551  GLPYTFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAV 730
            GLPY F RTGWL  ++ +  V++ T+H MMLL+ TR++L+    G F K+ SFGDLGFAV
Sbjct: 60   GLPYAFKRTGWLMGVLLLLSVSVLTHHCMMLLVHTRRRLDSFNGGGFSKLGSFGDLGFAV 119

Query: 731  CGSVGRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXP------KILGVSA 892
            CGS+GR  VD  ++LSQAGFCVGYLIFIG                        + LG+S+
Sbjct: 120  CGSLGRLVVDLFIILSQAGFCVGYLIFIGTTLANIFDPDSPTSLRHQITRLGSEFLGLSS 179

Query: 893  KSVYIWSCFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAF 1072
            KS+YIW CFPFQLGLNSI +LT LAPLSIFAD+VDL AM VV+V+D+II  +Q P V AF
Sbjct: 180  KSLYIWGCFPFQLGLNSIKTLTHLAPLSIFADVVDLAAMAVVVVEDSIIILKQRPDVVAF 239

Query: 1073 GSLSTFFYGLGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXX 1252
            G  S F YG+GVAVYSFEGVGM LPLESEMKDK KFGK+L L M FI             
Sbjct: 240  GGFSLFLYGMGVAVYSFEGVGMVLPLESEMKDKDKFGKVLALGMGFISLIYIAFGFLGYL 299

Query: 1253 XXXENTKDIITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMR 1432
               E+T DIITGN+G GL+STIVQLGLCINLFFTFPLMMNPV+E+ ERRF  G Y  W+R
Sbjct: 300  AFGEDTMDIITGNLGAGLISTIVQLGLCINLFFTFPLMMNPVFEIVERRFSGGMYSAWLR 359

Query: 1433 WMXXXXXXXXXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGI 1612
            W+            PNFTDFLSLVGSSTCC+LGFVLPALFH L FK+EM W++ S D+ I
Sbjct: 360  WLLVLAVTLVALFVPNFTDFLSLVGSSTCCILGFVLPALFHLLVFKEEMGWKQWSLDMAI 419

Query: 1613 IALGVFLGVSGTWYSLLEIFSVKV 1684
            + LGV L VSGTW SL EIFSVKV
Sbjct: 420  VGLGVVLAVSGTWSSLSEIFSVKV 443


>ref|XP_013731901.1| PREDICTED: amino acid transporter ANTL2 [Brassica napus]
          Length = 442

 Score =  520 bits (1340), Expect = e-177
 Identities = 273/444 (61%), Positives = 316/444 (71%), Gaps = 10/444 (2%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSLP-REDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLP 559
            MGF K+  + SS+ SL+  P REDTPLL G   P  LSSQ KTFANVFIA+VGAGVLGLP
Sbjct: 1    MGFAKEASSSSSSYSLKMPPPREDTPLLLGKSPP--LSSQFKTFANVFIAVVGAGVLGLP 58

Query: 560  YTFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLED-SPVGAFPKIASFGDLGFAVCG 736
            Y F RTGWL  ++ +  V++ T+H MMLL+ TR+KL+  +  G F KI SFGDLGFAVCG
Sbjct: 59   YAFKRTGWLMGVLLLLSVSVLTHHCMMLLVHTRRKLDSFNGGGGFSKIGSFGDLGFAVCG 118

Query: 737  SVGRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXX--------PKILGVSA 892
            S+GR  VD  ++LSQAGFCVGYLIFIG                          + LGVS+
Sbjct: 119  SLGRLVVDLFIILSQAGFCVGYLIFIGTTLANIFDPDSPTSLSLRHQITTLGSEFLGVSS 178

Query: 893  KSVYIWSCFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAF 1072
            KS+YIW CFPFQLGLNSI +LT LAPLSIFAD+VDL AM VV+V+D++I  +Q P V AF
Sbjct: 179  KSLYIWGCFPFQLGLNSIKTLTHLAPLSIFADVVDLAAMAVVVVEDSMIILKQRPDVVAF 238

Query: 1073 GSLSTFFYGLGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXX 1252
            G  S FFYG+GVAVYSFEGVGM LPLESEMKDK KFGK+L L M FI             
Sbjct: 239  GGFSLFFYGMGVAVYSFEGVGMVLPLESEMKDKDKFGKVLALGMGFISFIYIAFGFLGYL 298

Query: 1253 XXXENTKDIITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMR 1432
               E+T DIITGN+G GL+STIVQLGLCINLFFTFPLMMNPV+E+ ERRF  G Y  W+R
Sbjct: 299  AFGEDTMDIITGNLGAGLISTIVQLGLCINLFFTFPLMMNPVFEIVERRFSGGMYSAWLR 358

Query: 1433 WMXXXXXXXXXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGI 1612
            W+            PNFTDFLSLVGSSTCC+LGFVLPALFH L FK+EM W++ S D+ I
Sbjct: 359  WLLVLAVTLVALFVPNFTDFLSLVGSSTCCILGFVLPALFHLLVFKEEMGWKQWSLDMAI 418

Query: 1613 IALGVFLGVSGTWYSLLEIFSVKV 1684
            + LGV L VSGTW SL EIFSVKV
Sbjct: 419  VGLGVVLAVSGTWSSLSEIFSVKV 442


>ref|XP_013595228.1| PREDICTED: amino acid transporter ANTL2 [Brassica oleracea var.
            oleracea]
          Length = 443

 Score =  520 bits (1340), Expect = e-177
 Identities = 271/444 (61%), Positives = 315/444 (70%), Gaps = 10/444 (2%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSL----PREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVL 550
            MGF K+  + SS++S  SL    PREDTPLL G   P  LSSQ KTFANVFIA+VGAGVL
Sbjct: 1    MGFAKEASSSSSSSSSYSLKMPPPREDTPLLLGKSPPP-LSSQFKTFANVFIAVVGAGVL 59

Query: 551  GLPYTFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAV 730
            GLPY F RTGWL  ++ +  V++ T+H MMLL+ TR++L+    G F KI SFGDLGFAV
Sbjct: 60   GLPYAFKRTGWLMGVLLLLSVSVLTHHCMMLLVHTRRRLDSFNGGGFSKIGSFGDLGFAV 119

Query: 731  CGSVGRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXP------KILGVSA 892
            CGS+GR  VD  ++LSQAGFCVGYLIFIG                        + LG+S+
Sbjct: 120  CGSLGRLVVDLFIILSQAGFCVGYLIFIGTTLANIFDPDSPTSLRHQITRLGSEFLGLSS 179

Query: 893  KSVYIWSCFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAF 1072
            KS+YIW CFPFQLGLNSI +LT LAPLSIFAD+VDL AM VV+V+D++I  +Q P V AF
Sbjct: 180  KSLYIWGCFPFQLGLNSIKTLTHLAPLSIFADVVDLAAMAVVVVEDSMIILKQRPDVVAF 239

Query: 1073 GSLSTFFYGLGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXX 1252
            G  S F YG+GVAVYSFEGVGM LPLESEMKDK KFGK+L L M FI             
Sbjct: 240  GGFSLFLYGMGVAVYSFEGVGMVLPLESEMKDKDKFGKVLALGMGFISLIYIAFGFLGYL 299

Query: 1253 XXXENTKDIITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMR 1432
               E+T DIITGN+G GL+STIVQLGLCINLFFTFPLMMNPV+E+ ERRF  G Y  W+R
Sbjct: 300  AFGEDTMDIITGNLGAGLISTIVQLGLCINLFFTFPLMMNPVFEIVERRFSGGMYSAWLR 359

Query: 1433 WMXXXXXXXXXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGI 1612
            W+            PNFTDFLSLVGSSTCC+LGFVLPALFH L FK+EM W++ S D+ I
Sbjct: 360  WLLVLAVTLVALFVPNFTDFLSLVGSSTCCILGFVLPALFHLLVFKEEMGWKQWSLDMAI 419

Query: 1613 IALGVFLGVSGTWYSLLEIFSVKV 1684
            + LGV L VSGTW SL EIFSVKV
Sbjct: 420  VGLGVVLAVSGTWSSLSEIFSVKV 443


>ref|XP_009629537.1| PREDICTED: proton-coupled amino acid transporter 3-like [Nicotiana
            tomentosiformis]
          Length = 428

 Score =  520 bits (1338), Expect = e-177
 Identities = 264/423 (62%), Positives = 313/423 (73%), Gaps = 1/423 (0%)
 Frame = +2

Query: 410  ESSNNSLRSLPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLPYTFMRTGWLT 589
            ESS   L  LP+E+TP+L  +      S  PKTFAN FIAIVGAGVLGLPYTFMRTGW+T
Sbjct: 13   ESSKGHL--LPKEETPILENSK-----SCLPKTFANFFIAIVGAGVLGLPYTFMRTGWIT 65

Query: 590  SLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGSVGRFAVDSMV 769
             L+T+F VA  TYHGMMLLI TRK+L+ S +G   KI+SFGDLGF VCG +GRFAVD M+
Sbjct: 66   GLVTLFSVAALTYHGMMLLIHTRKRLDLSLIGD-SKISSFGDLGFVVCGPIGRFAVDVMI 124

Query: 770  VLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSVYIWSCFPFQLGLNSIP 949
            VL+Q GFC+GYLIFIGN                   L  SAKS+YIW CFPFQLGL SIP
Sbjct: 125  VLAQVGFCIGYLIFIGNTLSHLLSLSKGFGIP----LFSSAKSLYIWGCFPFQLGLTSIP 180

Query: 950  SLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPV-VEAFGSLSTFFYGLGVAVYSFE 1126
            SLTLLAPLSIFAD+  +GAMGVVMV+D + FT Q P  + AFG++STFFYGLGVA+YSFE
Sbjct: 181  SLTLLAPLSIFADVAQIGAMGVVMVEDVLTFTNQWPSDIHAFGTVSTFFYGLGVALYSFE 240

Query: 1127 GVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDIITGNMGKGL 1306
            GVGMA+PLE+EMKDKSK+GKIL L+M  I                 +TKDI+T NM KG+
Sbjct: 241  GVGMAIPLEAEMKDKSKYGKILALSMFLIALMYGTFGVMGYFAFGSSTKDIVTSNMAKGI 300

Query: 1307 LSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXXXXXXXPNFT 1486
            LST+V++GLCINLFFTFPLM+NP +EVFERRF  G YC+WMRW+            PNF 
Sbjct: 301  LSTLVKVGLCINLFFTFPLMLNPAFEVFERRFCDGNYCVWMRWILVLGVTLVALLVPNFA 360

Query: 1487 DFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGVSGTWYSLLE 1666
            DF+SLVGSSTCC LGF+LPALFH+  FK++M  + A  D+G+IALGVF GVSGTWYSL+E
Sbjct: 361  DFMSLVGSSTCCALGFILPALFHYQVFKEDMDRKGAFFDLGLIALGVFFGVSGTWYSLVE 420

Query: 1667 IFS 1675
            +F+
Sbjct: 421  MFA 423


>ref|XP_010066350.1| PREDICTED: proton-coupled amino acid transporter 3-like [Eucalyptus
            grandis] gi|629098461|gb|KCW64226.1| hypothetical protein
            EUGRSUZ_G01876 [Eucalyptus grandis]
          Length = 428

 Score =  520 bits (1338), Expect = e-177
 Identities = 272/433 (62%), Positives = 307/433 (70%), Gaps = 3/433 (0%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSLPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLPY 562
            MGF K  EA SS+ +L  +PREDTPLL    KP  LSSQ KTFANVFIAIVGAGVLGLPY
Sbjct: 1    MGFDK--EAGSSSRTLTPIPREDTPLLG---KPRQLSSQAKTFANVFIAIVGAGVLGLPY 55

Query: 563  TFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGSV 742
             F RTGWLT L+ +F +A  TYH MMLL+RTR++LE   V  F KIASFGDLGF VCG +
Sbjct: 56   AFKRTGWLTGLLMLFSIAALTYHCMMLLVRTRRRLES--VDGFSKIASFGDLGFTVCGWI 113

Query: 743  GRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXX---PKILGVSAKSVYIWS 913
            GR  VD M+VLSQAGFCV YLIFIGN                     I G+SAKS YIW 
Sbjct: 114  GRVVVDIMMVLSQAGFCVSYLIFIGNTMANLFNSGPATHVAAYVSSSIWGISAKSFYIWG 173

Query: 914  CFPFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTFF 1093
            C PFQLG+NSI +LT LAPLSIFAD+VDLGAMGVVM  +A I  +    V+AFG LS FF
Sbjct: 174  CLPFQLGINSIKTLTHLAPLSIFADVVDLGAMGVVMADEAGIVWKNGANVQAFGGLSVFF 233

Query: 1094 YGLGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTK 1273
            YG+GVAVY+FEG+GMAL LESEM+DK KFGKIL + M FI                 NT+
Sbjct: 234  YGMGVAVYAFEGIGMALHLESEMEDKRKFGKILAIAMAFIALMYGGFGLLGYLAFGANTR 293

Query: 1274 DIITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXX 1453
            DIIT NMGKGL+STIVQLGLCINLFFTFPLMM+PVYE+ ERR W G+YC+W+RW+     
Sbjct: 294  DIITANMGKGLISTIVQLGLCINLFFTFPLMMHPVYEIVERRLWGGRYCIWLRWLLVLLV 353

Query: 1454 XXXXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFL 1633
                   PNF DFLSLVGSS CC LGFVLP+LFH L  K+EM W   S DVGI  LG+ L
Sbjct: 354  SLVALMVPNFGDFLSLVGSSVCCGLGFVLPSLFHLLVCKEEMGWIGLSTDVGIFVLGLVL 413

Query: 1634 GVSGTWYSLLEIF 1672
             VSGTWY+L EIF
Sbjct: 414  AVSGTWYALTEIF 426


>ref|XP_008448189.1| PREDICTED: proton-coupled amino acid transporter 3-like [Cucumis
            melo]
          Length = 427

 Score =  518 bits (1334), Expect = e-176
 Identities = 267/435 (61%), Positives = 314/435 (72%), Gaps = 1/435 (0%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRS-LPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLP 559
            MGF +  EA SS+N LRS + REDTPLL     P  LSSQPKTFANVFIAIVGAGVLGLP
Sbjct: 1    MGFDR--EASSSSNRLRSTVAREDTPLLGA---PLPLSSQPKTFANVFIAIVGAGVLGLP 55

Query: 560  YTFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGS 739
            Y F RTGW+ SL+ +F V+  TY+ MMLL+ TR+K+E   V  F KI SFGDLG+ +CGS
Sbjct: 56   YAFKRTGWVMSLLMLFSVSFLTYYCMMLLVYTRRKIES--VIGFSKINSFGDLGYTICGS 113

Query: 740  VGRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSVYIWSCF 919
             GRF VD +++LSQ GFCVGYLIFIGN                PKILG++ K  Y+W CF
Sbjct: 114  PGRFIVDLLIILSQTGFCVGYLIFIGNTMADVFNSPTVMDLN-PKILGLAPKVAYVWGCF 172

Query: 920  PFQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTFFYG 1099
            PFQLGLNSI +LT LAPLSIFADIVDLGAM VVMV+D +I   Q   VEAFG  S FFYG
Sbjct: 173  PFQLGLNSIQTLTHLAPLSIFADIVDLGAMVVVMVKDVLIIFEQSQSVEAFGGFSVFFYG 232

Query: 1100 LGVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDI 1279
            +GVAVY+FEG+GM LPLESE KDK KFG++LGL+M FI                ++TKD+
Sbjct: 233  MGVAVYAFEGIGMVLPLESETKDKEKFGRVLGLSMAFITVLYGAFGTLGYFAFGKDTKDM 292

Query: 1280 ITGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXX 1459
            IT N+G G +ST+V+LGLCINLFFT PLMMNPVYE+ ERRFW G+YCLW+RW+       
Sbjct: 293  ITSNLGPGFISTVVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYCLWLRWLLVFLVSL 352

Query: 1460 XXXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGV 1639
                 PNF DFLSLVGS+ CC L FVLPALFHFL FK E+  +    D+GI+ LG+ LGV
Sbjct: 353  VALLVPNFADFLSLVGSAVCCALAFVLPALFHFLVFKQELDIKGWCLDIGILVLGLVLGV 412

Query: 1640 SGTWYSLLEIFSVKV 1684
            SGTW +L+EIFSVKV
Sbjct: 413  SGTWSALVEIFSVKV 427


>ref|XP_009801334.1| PREDICTED: proton-coupled amino acid transporter 3-like [Nicotiana
            sylvestris]
          Length = 426

 Score =  516 bits (1329), Expect = e-176
 Identities = 264/433 (60%), Positives = 315/433 (72%)
 Frame = +2

Query: 383  MGFKKQNEAESSNNSLRSLPREDTPLLSGNDKPHILSSQPKTFANVFIAIVGAGVLGLPY 562
            MGF+K N+A SS++ L+ +PREDTPLL+ N     LSS  KTFANVFIAIVGAGVLGLPY
Sbjct: 1    MGFEKNNKASSSSHVLQ-IPREDTPLLAKNQH---LSSPSKTFANVFIAIVGAGVLGLPY 56

Query: 563  TFMRTGWLTSLITIFFVALFTYHGMMLLIRTRKKLEDSPVGAFPKIASFGDLGFAVCGSV 742
            TF RTGW+   I +F VA  TYH MMLL+ +R+K+E     +  +I+SFGDLGFAVCG +
Sbjct: 57   TFKRTGWIMGAIMLFSVAFLTYHCMMLLVFSRRKIESHLKTS--EISSFGDLGFAVCGPI 114

Query: 743  GRFAVDSMVVLSQAGFCVGYLIFIGNXXXXXXXXXXXXXXXXPKILGVSAKSVYIWSCFP 922
            GRFAVD+M+VLSQAGFC+ Y+IFI N                PKILG   K+VYIWSCFP
Sbjct: 115  GRFAVDAMIVLSQAGFCISYMIFIANTLAYCFNYSKSNPS--PKILGFPPKTVYIWSCFP 172

Query: 923  FQLGLNSIPSLTLLAPLSIFADIVDLGAMGVVMVQDAIIFTRQMPVVEAFGSLSTFFYGL 1102
            FQLGLNSIPSLTLLAPLSIFAD+V+LGAMGVVMV+D +++ +   ++EAFG  S FFYGL
Sbjct: 173  FQLGLNSIPSLTLLAPLSIFADVVELGAMGVVMVEDVMVYLKSSHILEAFGGFSVFFYGL 232

Query: 1103 GVAVYSFEGVGMALPLESEMKDKSKFGKILGLTMVFIXXXXXXXXXXXXXXXXENTKDII 1282
            GVAVY+FEGVGM LPLE+EMK+K+KFGKILGL+M  I                E TKDII
Sbjct: 233  GVAVYAFEGVGMVLPLEAEMKNKAKFGKILGLSMALISLMYGAFGVLGYFAFGEETKDII 292

Query: 1283 TGNMGKGLLSTIVQLGLCINLFFTFPLMMNPVYEVFERRFWHGKYCLWMRWMXXXXXXXX 1462
            T N+G+G LS++VQ+GLCINLFFTFPLMMNPVYEV ERRF  G+YCLW+RW+        
Sbjct: 293  TTNLGQGWLSSLVQIGLCINLFFTFPLMMNPVYEVMERRFCEGRYCLWIRWIVVLVVTIV 352

Query: 1463 XXXXPNFTDFLSLVGSSTCCVLGFVLPALFHFLTFKDEMSWRRASADVGIIALGVFLGVS 1642
                PNF DFLSLVGSS C +LGFVLPALFH + FKDE+ W   + D  II +     V 
Sbjct: 353  ALLVPNFADFLSLVGSSVCIILGFVLPALFHLIVFKDELRWHGLACDGAIIVMATVFSVY 412

Query: 1643 GTWYSLLEIFSVK 1681
            GT+ S+LEIF  K
Sbjct: 413  GTFSSMLEIFGAK 425