BLASTX nr result
ID: Rehmannia26_contig00030167
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia26_contig00030167
(406 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006348011.1| PREDICTED: uncharacterized protein LOC102587... 117 2e-24
ref|XP_004231640.1| PREDICTED: uncharacterized protein LOC101266... 117 2e-24
gb|EMJ11759.1| hypothetical protein PRUPE_ppa014600mg [Prunus pe... 115 5e-24
gb|EOY22023.1| Uncharacterized protein TCM_014176 [Theobroma cacao] 112 4e-23
gb|EOX94693.1| Uncharacterized protein TCM_004305 [Theobroma cacao] 112 5e-23
ref|XP_003637710.1| Dual specificity protein phosphatase [Medica... 112 5e-23
gb|EOY27578.1| Uncharacterized protein TCM_029406 [Theobroma cacao] 109 3e-22
emb|CAN65842.1| hypothetical protein VITISV_027369 [Vitis vinifera] 108 1e-21
ref|XP_006494998.1| PREDICTED: uncharacterized protein LOC102629... 107 2e-21
gb|EOY18929.1| Retrotransposon protein, putative [Theobroma cacao] 106 4e-21
gb|EOX94441.1| Uncharacterized protein TCM_004024 [Theobroma cacao] 105 5e-21
ref|XP_006493601.1| PREDICTED: putative nuclease HARBI1-like iso... 104 1e-20
ref|XP_006444396.1| hypothetical protein CICLE_v10023542mg, part... 102 4e-20
ref|XP_006478124.1| PREDICTED: putative nuclease HARBI1-like [Ci... 96 4e-18
gb|AFW74633.1| hypothetical protein ZEAMMB73_204913 [Zea mays] 95 9e-18
gb|AFW82750.1| hypothetical protein ZEAMMB73_878123 [Zea mays] 95 1e-17
gb|ADN33754.1| retrotransposon protein [Cucumis melo subsp. melo] 94 2e-17
emb|CAN62415.1| hypothetical protein VITISV_032219 [Vitis vinifera] 92 6e-17
ref|XP_006471324.1| PREDICTED: uncharacterized protein LOC102618... 90 3e-16
gb|EMJ14692.1| hypothetical protein PRUPE_ppa022346mg, partial [... 90 4e-16
>ref|XP_006348011.1| PREDICTED: uncharacterized protein LOC102587703 [Solanum tuberosum]
Length = 285
Score = 117 bits (293), Expect = 2e-24
Identities = 56/134 (41%), Positives = 84/134 (62%)
Frame = -1
Query: 403 RQSNQLVKYNMISRIPAQVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDT 224
+++ +++Y M SR+P +SHL I+ SD CI+ LRM RN+F L L + VGG+TD+
Sbjct: 40 KRNRHVIRYLMSSRVPKIISHLNCIVQDSDTTCIDKLRMDRNSFHTLVLLTKEVGGLTDS 99
Query: 223 RNVRVHEKVAMFLSVLGHHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDP 44
+ + EK+AMFL++L HH KNR +K D++RSG++VS+ F+E L P P
Sbjct: 100 KYMSSSEKLAMFLNILAHHEKNRSIKVDYIRSGWSVSQAFNECLKAILKLAPLLLVNPKP 159
Query: 43 VPEHCTIESWKWFK 2
V E + W+WFK
Sbjct: 160 VLEDELDDRWRWFK 173
>ref|XP_004231640.1| PREDICTED: uncharacterized protein LOC101266775 [Solanum
lycopersicum]
Length = 315
Score = 117 bits (293), Expect = 2e-24
Identities = 57/128 (44%), Positives = 84/128 (65%)
Frame = -1
Query: 385 VKYNMISRIPAQVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVH 206
++Y M +RI +SHL +I +D+ CI+ LRM RNAF L L +N+GG+TD++N+
Sbjct: 48 IRYCMSARIQKILSHLNVLIRDNDIVCIDKLRMDRNAFHILASLAKNIGGLTDSKNMSST 107
Query: 205 EKVAMFLSVLGHHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCT 26
EK+AMFL++L HH KNR +K D++RSG++VS+ F+E L P+PV E +
Sbjct: 108 EKLAMFLNILAHHEKNRSIKVDYIRSGWSVSRAFNECLRAILKLTPVLLVKPNPVLEADS 167
Query: 25 IESWKWFK 2
+ WKWFK
Sbjct: 168 DDRWKWFK 175
>gb|EMJ11759.1| hypothetical protein PRUPE_ppa014600mg [Prunus persica]
Length = 691
Score = 115 bits (289), Expect = 5e-24
Identities = 55/117 (47%), Positives = 72/117 (61%)
Frame = -1
Query: 352 QVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVLG 173
Q+ +L ++ SD CI+ LRM R +F KLC +L G + TRN+ E VA+FL++L
Sbjct: 17 QLEYLHGLVYESDTTCIDQLRMDRQSFHKLCQILVTKGELRSTRNMSTEEMVAIFLNILA 76
Query: 172 HHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
HH KNRV+K +F RSG TVSKYFHE +P+PVPE+ T WKWFK
Sbjct: 77 HHHKNRVIKFNFTRSGRTVSKYFHECLKAMIRCQKDFWKSPEPVPENSTDYRWKWFK 133
>gb|EOY22023.1| Uncharacterized protein TCM_014176 [Theobroma cacao]
Length = 706
Score = 112 bits (281), Expect = 4e-23
Identities = 48/114 (42%), Positives = 75/114 (65%)
Frame = -1
Query: 343 HLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVLGHHR 164
++RR++ +D+ CI +RM+R FLKLC +LE++GG+ T+N+ V E+VA+FL ++ HH
Sbjct: 63 YVRRLVYDNDISCISQIRMNRVTFLKLCEMLESIGGLKSTKNMLVDEQVAIFLHIIAHHV 122
Query: 163 KNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
KNRV+ +F RSG ++S++FH V P+P+P + T WKWFK
Sbjct: 123 KNRVISLNFRRSGESISRHFHNVLAAVLKLQEHLFRKPEPIPTNSTDNQWKWFK 176
>gb|EOX94693.1| Uncharacterized protein TCM_004305 [Theobroma cacao]
Length = 502
Score = 112 bits (280), Expect = 5e-23
Identities = 48/114 (42%), Positives = 75/114 (65%)
Frame = -1
Query: 343 HLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVLGHHR 164
++RR++ +D+ CI +RM+R FLKLC +LE++GG+ T+N+ V E+VA+FL ++ HH
Sbjct: 63 YVRRLVYDNDISCISQIRMNRVTFLKLCEMLESIGGLKSTKNMLVDEQVAIFLHIIAHHV 122
Query: 163 KNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
KNRV+ +F RSG ++S++FH V P+P+P + T WKWFK
Sbjct: 123 KNRVISLNFRRSGESISRHFHNVLAAVLKLQEHLFRKPEPIPTNSTDNRWKWFK 176
>ref|XP_003637710.1| Dual specificity protein phosphatase [Medicago truncatula]
gi|355503645|gb|AES84848.1| Dual specificity protein
phosphatase [Medicago truncatula]
Length = 399
Score = 112 bits (280), Expect = 5e-23
Identities = 59/123 (47%), Positives = 72/123 (58%)
Frame = -1
Query: 370 ISRIPAQVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAM 191
++R ++ L IIN SD CI LRM RN F LC +L GG+ R V V EKVA+
Sbjct: 150 LTRYESRRDILDSIINASDSHCIWELRMCRNTFAHLCEVLRVRGGLVKLRQVTVEEKVAV 209
Query: 190 FLSVLGHHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWK 11
FL+VL HH KNR ++ RSG T+SKY H V LA P+PVP+ CT E WK
Sbjct: 210 FLNVLAHHTKNRSIQVRLSRSGQTISKYCHRVLAAVLRLCNDLLAKPEPVPQDCTDERWK 269
Query: 10 WFK 2
WFK
Sbjct: 270 WFK 272
>gb|EOY27578.1| Uncharacterized protein TCM_029406 [Theobroma cacao]
Length = 516
Score = 109 bits (273), Expect = 3e-22
Identities = 46/114 (40%), Positives = 74/114 (64%)
Frame = -1
Query: 343 HLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVLGHHR 164
++RR++ +D+ CI +RM+R F KLC +LE++GG+ T+N+ V E+VA+FL ++ HH
Sbjct: 63 YVRRLVYDNDISCISQIRMNRVTFFKLCEMLESIGGLKSTKNMLVDEQVAIFLHIIAHHV 122
Query: 163 KNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
KNRV+ +F +SG ++S++FH V P+P+P + T WKWFK
Sbjct: 123 KNRVISLNFRKSGESISRHFHNVLAAVLKLQEYLFRKPEPIPTNSTDNRWKWFK 176
>emb|CAN65842.1| hypothetical protein VITISV_027369 [Vitis vinifera]
Length = 579
Score = 108 bits (269), Expect = 1e-21
Identities = 53/117 (45%), Positives = 74/117 (63%)
Frame = -1
Query: 352 QVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVLG 173
+V +L R+I SDV C+E LRM R+ F LC +L +G + D++ + V E VA+FL +L
Sbjct: 53 RVENLNRLIYGSDVACMEQLRMDRHTFTTLCSMLRTIGKLKDSKYIDVEEMVALFLHILA 112
Query: 172 HHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
HH KNRV+K F+RSG T+S++F+ V L P+PV E+ T E WKWFK
Sbjct: 113 HHVKNRVIKFRFLRSGETISRHFNAVLNAVIRLQGVLLKKPEPVSENSTDERWKWFK 169
>ref|XP_006494998.1| PREDICTED: uncharacterized protein LOC102629376 [Citrus sinensis]
Length = 283
Score = 107 bits (267), Expect = 2e-21
Identities = 56/136 (41%), Positives = 74/136 (54%), Gaps = 2/136 (1%)
Frame = -1
Query: 403 RQSNQ--LVKYNMISRIPAQVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGIT 230
R+ NQ L++ ++ +L II SD+ C+ LRM R F LC LL GG+
Sbjct: 32 RKHNQRKLIRSRTFQNHATRLQYLDSIIGNSDIQCVNQLRMDRRTFGLLCELLRVNGGLR 91
Query: 229 DTRNVRVHEKVAMFLSVLGHHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATP 50
+V + E+V MFL L HH KNR + F RSG TVS+YF+ + L P
Sbjct: 92 ANGSVTIEEQVCMFLHTLSHHVKNRTIGSRFFRSGETVSRYFNSILNGVLRLHDVLLRAP 151
Query: 49 DPVPEHCTIESWKWFK 2
+PVPE+CT E WKWFK
Sbjct: 152 EPVPENCTDERWKWFK 167
>gb|EOY18929.1| Retrotransposon protein, putative [Theobroma cacao]
Length = 312
Score = 106 bits (264), Expect = 4e-21
Identities = 47/118 (39%), Positives = 77/118 (65%)
Frame = -1
Query: 355 AQVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVL 176
A ++ R+++ +D CI +RM+++ F+KLC +LEN+GG+ TRN+ V E+VA+FL ++
Sbjct: 58 ANREYVMRLVHDNDFSCIAQVRMNKHTFVKLCEMLENIGGLKSTRNMLVDEQVAIFLYII 117
Query: 175 GHHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
HH K++V++Q F RS T+S++F++V L P P+P + T WKWFK
Sbjct: 118 AHHLKSQVIRQKFKRSSKTISRHFNKVLNAVMKLQDHLLRKPKPIPTNSTDNWWKWFK 175
>gb|EOX94441.1| Uncharacterized protein TCM_004024 [Theobroma cacao]
Length = 357
Score = 105 bits (263), Expect = 5e-21
Identities = 46/118 (38%), Positives = 72/118 (61%)
Frame = -1
Query: 355 AQVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVL 176
A ++RR++ +D+ CI + M+R AF KLC +L+++GG+ T+N+ V E V +FL ++
Sbjct: 41 ANREYVRRLVYDNDISCISQIMMNRAAFFKLCEMLKSIGGLKSTKNMLVDEHVVIFLHII 100
Query: 175 GHHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
HH KNRV+ +F RSG T+S++FH V P+ +P + T WKWFK
Sbjct: 101 AHHVKNRVISLNFKRSGETISRHFHNVLNAVLKLQKHLFKKPESIPTNSTDNRWKWFK 158
>ref|XP_006493601.1| PREDICTED: putative nuclease HARBI1-like isoform X1 [Citrus
sinensis] gi|568881482|ref|XP_006493602.1| PREDICTED:
putative nuclease HARBI1-like isoform X2 [Citrus
sinensis]
Length = 393
Score = 104 bits (259), Expect = 1e-20
Identities = 54/124 (43%), Positives = 69/124 (55%)
Frame = -1
Query: 376 NMISRIPAQVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKV 197
N R+ Q+SHL ++ SD+ C+ LRM R AF KLC LL G + ++ V E V
Sbjct: 48 NSAYRVYQQLSHLHGLVFESDIKCLSQLRMDRQAFFKLCKLLCEKGSLVRSKRVSPEEMV 107
Query: 196 AMFLSVLGHHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIES 17
AMFLS+L HH KNRVV +F RS TVSK FHE P+P+ ++ T
Sbjct: 108 AMFLSILAHHVKNRVVGFNFKRSRRTVSKCFHECLRAMIRCQKEFWKKPEPITDNSTDPK 167
Query: 16 WKWF 5
WKWF
Sbjct: 168 WKWF 171
>ref|XP_006444396.1| hypothetical protein CICLE_v10023542mg, partial [Citrus clementina]
gi|557546658|gb|ESR57636.1| hypothetical protein
CICLE_v10023542mg, partial [Citrus clementina]
Length = 309
Score = 102 bits (255), Expect = 4e-20
Identities = 53/120 (44%), Positives = 67/120 (55%)
Frame = -1
Query: 364 RIPAQVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFL 185
R+ Q+SHL ++ SD+ C+ LRM R AF KLC LL G + ++ V E VAMFL
Sbjct: 169 RVYQQLSHLHGLVFESDIKCLSQLRMDRQAFFKLCKLLCEKGSLVRSKRVSPEEMVAMFL 228
Query: 184 SVLGHHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWF 5
S+L HH KNRVV +F RS TVSK FHE P+P+ + T WKWF
Sbjct: 229 SILAHHVKNRVVGFNFKRSRRTVSKCFHECLRAMIRCQKEFWKKPEPITNNSTDPKWKWF 288
>ref|XP_006478124.1| PREDICTED: putative nuclease HARBI1-like [Citrus sinensis]
Length = 370
Score = 96.3 bits (238), Expect = 4e-18
Identities = 50/118 (42%), Positives = 65/118 (55%)
Frame = -1
Query: 355 AQVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVL 176
+Q+ +L II D+ C+ LRM R F LC LL + G + V V E+V MFL +L
Sbjct: 29 SQLHYLDSIIGNGDLQCVHQLRMDRRTFGLLCELLRSRGKVKADGLVTVEEQVCMFLHIL 88
Query: 175 GHHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
HH KNR + F RSG TVS+YF+ V L P+PVPE+ T W+WFK
Sbjct: 89 SHHVKNRTISSRFFRSGETVSRYFNSVLKGVLRLQSLLLKAPEPVPENYTDGRWRWFK 146
>gb|AFW74633.1| hypothetical protein ZEAMMB73_204913 [Zea mays]
Length = 288
Score = 95.1 bits (235), Expect = 9e-18
Identities = 51/116 (43%), Positives = 66/116 (56%)
Frame = -1
Query: 349 VSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVLGH 170
+ +L +I SD CI LRM R F LC +L +VGG+ TRN+ + E VA FL L H
Sbjct: 1 MENLNGLIRESDRKCISELRMDRRTFFILCEMLRDVGGLKATRNMTLEEIVAHFLYTLAH 60
Query: 169 HRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
H KNR + + F RSG TVS+ F+ L TPDP+PE+ T +WK FK
Sbjct: 61 HLKNRTIGRFFFRSGETVSRQFNLCLLAVLKLQHLLLKTPDPIPENSTDNTWKNFK 116
>gb|AFW82750.1| hypothetical protein ZEAMMB73_878123 [Zea mays]
Length = 445
Score = 94.7 bits (234), Expect = 1e-17
Identities = 50/116 (43%), Positives = 65/116 (56%)
Frame = -1
Query: 349 VSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVLGH 170
+ +L +I SD CI LRM F LC +L +VGG+ TRN+ + E VA FL L H
Sbjct: 1 MENLNELIRESDRKCISELRMDTRTFFILCEMLRDVGGLKATRNMTLEEVVAQFLYTLAH 60
Query: 169 HRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
H KNR + + F RSG TVS+ F+ L TPDP+PE+ T +WK FK
Sbjct: 61 HLKNRTIGRFFFRSGETVSRQFNLCLLAVLKLQHLLLKTPDPIPENSTDNTWKNFK 116
>gb|ADN33754.1| retrotransposon protein [Cucumis melo subsp. melo]
Length = 623
Score = 94.0 bits (232), Expect = 2e-17
Identities = 49/110 (44%), Positives = 67/110 (60%)
Frame = -1
Query: 331 IINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVLGHHRKNRV 152
+I+ SD+ C ++ RM R F LC+LL NV G++ T V V E VAMFL VL H KNRV
Sbjct: 1 MIHESDLVCRQSTRMDRRTFAILCHLLRNVAGLSSTEIVDVEEMVAMFLHVLAHDVKNRV 60
Query: 151 VKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
++Q+FVRSG TVS++F+ V + P PV +C + WK F+
Sbjct: 61 IQQEFVRSGETVSRHFNIVLLAVLRLYEELIKRPVPVTSNCNDQRWKCFE 110
>emb|CAN62415.1| hypothetical protein VITISV_032219 [Vitis vinifera]
Length = 185
Score = 92.4 bits (228), Expect = 6e-17
Identities = 45/101 (44%), Positives = 62/101 (61%)
Frame = -1
Query: 304 IENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVLGHHRKNRVVKQDFVRSG 125
+E LRM R+ F LC +L +G + D++ V V E VA+FL +L HH KNRV+K F+RSG
Sbjct: 1 MEQLRMDRHTFTMLCSMLRTIGKLKDSKYVDVEEMVALFLHILAHHVKNRVIKFRFLRSG 60
Query: 124 YTVSKYFHEVXXXXXXXXXXXLATPDPVPEHCTIESWKWFK 2
T+S++F+ V L P+PV E+ E WKWFK
Sbjct: 61 ETISRHFNAVLNAVIRLQGVLLKKPEPVSENSIDERWKWFK 101
>ref|XP_006471324.1| PREDICTED: uncharacterized protein LOC102618885 [Citrus sinensis]
Length = 167
Score = 90.1 bits (222), Expect = 3e-16
Identities = 47/112 (41%), Positives = 63/112 (56%)
Frame = -1
Query: 370 ISRIPAQVSHLRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAM 191
++R+ ++S L +I SDV C+ LRM R F KLC +L GG+ + NV E +AM
Sbjct: 50 VNRLWTRMSFLYGLIFDSDVKCLSQLRMDRQTFTKLCNMLREEGGLVGSTNVSPEEMLAM 109
Query: 190 FLSVLGHHRKNRVVKQDFVRSGYTVSKYFHEVXXXXXXXXXXXLATPDPVPE 35
FL +L HH KN V+ +F RSG T+SK FHE TP+PV E
Sbjct: 110 FLYILAHHMKNWVIGFNFTRSGRTISKSFHECLKAMIRCQKNFWKTPEPVME 161
>gb|EMJ14692.1| hypothetical protein PRUPE_ppa022346mg, partial [Prunus persica]
Length = 364
Score = 89.7 bits (221), Expect = 4e-16
Identities = 42/82 (51%), Positives = 58/82 (70%)
Frame = -1
Query: 340 LRRIINISDVDCIENLRMSRNAFLKLCYLLENVGGITDTRNVRVHEKVAMFLSVLGHHRK 161
+ R+I +D C++ LRM + F KLC +L G + DTRNV V E VA+FL+VL HH+K
Sbjct: 1 MNRMIYETDRRCVDELRMDLHTFSKLCSMLRATGRLHDTRNVSVDEMVAVFLNVLAHHKK 60
Query: 160 NRVVKQDFVRSGYTVSKYFHEV 95
NRV++ DF RSG T+S+YF+ V
Sbjct: 61 NRVIQNDFYRSGETISRYFNAV 82