BLASTX nr result
ID: Rehmannia26_contig00027407
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia26_contig00027407
(354 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002332187.1| predicted protein [Populus trichocarpa] gi|5... 125 6e-27
ref|XP_002513408.1| conserved hypothetical protein [Ricinus comm... 114 1e-23
gb|EOY13767.1| SBP family protein, putative [Theobroma cacao] 112 5e-23
gb|ABK96244.1| unknown [Populus trichocarpa x Populus deltoides] 109 3e-22
ref|XP_002317616.1| hypothetical protein POPTR_0011s14590g [Popu... 109 4e-22
gb|EMJ12699.1| hypothetical protein PRUPE_ppa007884mg [Prunus pe... 99 6e-19
ref|XP_004171715.1| PREDICTED: uncharacterized LOC101212862, par... 92 9e-17
ref|XP_004139826.1| PREDICTED: uncharacterized protein LOC101212... 92 9e-17
gb|EXB53388.1| Baculoviral IAP repeat-containing protein 7-A [Mo... 90 3e-16
ref|XP_004488822.1| PREDICTED: uncharacterized protein LOC101497... 88 1e-15
ref|XP_004294111.1| PREDICTED: uncharacterized protein LOC101297... 82 7e-14
gb|AFK43980.1| unknown [Medicago truncatula] 79 6e-13
gb|ACJ85143.1| unknown [Medicago truncatula] 79 6e-13
ref|XP_003596191.1| S-RNase binding protein [Medicago truncatula... 79 6e-13
gb|EOY27519.1| SBP family protein, putative isoform 2, partial [... 77 2e-12
gb|EOY27518.1| SBP family protein, putative isoform 1 [Theobroma... 75 1e-11
ref|XP_006441059.1| hypothetical protein CICLE_v10021015mg [Citr... 70 3e-10
ref|XP_006478050.1| PREDICTED: probable BOI-related E3 ubiquitin... 69 7e-10
ref|XP_002266864.2| PREDICTED: uncharacterized protein LOC100255... 68 1e-09
emb|CBI21767.3| unnamed protein product [Vitis vinifera] 68 1e-09
>ref|XP_002332187.1| predicted protein [Populus trichocarpa]
gi|566154467|ref|XP_006370471.1| hypothetical protein
POPTR_0001s43020g [Populus trichocarpa]
gi|550349664|gb|ERP67040.1| hypothetical protein
POPTR_0001s43020g [Populus trichocarpa]
Length = 337
Score = 125 bits (314), Expect = 6e-27
Identities = 66/104 (63%), Positives = 77/104 (74%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPMRYGLAAPLSGTTTATETVVPMYG 173
MAVEARHLNLFPPQ+L NR +MMN IE +Y T M YG+ PLSGTTT ETV+PMY
Sbjct: 1 MAVEARHLNLFPPQLLSNREMMMNPIEADTYMYNTQMGYGV--PLSGTTTTAETVLPMYS 58
Query: 172 STITGTVPAKTAAIKSDSGLSYAVPVSRKRSRDAINPLVSSSFP 41
S IT ++P KT IKS+SGL+Y +PV RKR RD INPL+S P
Sbjct: 59 SVITDSIPHKT-QIKSESGLTYNLPVQRKRPRDTINPLLSYPIP 101
>ref|XP_002513408.1| conserved hypothetical protein [Ricinus communis]
gi|223547316|gb|EEF48811.1| conserved hypothetical
protein [Ricinus communis]
Length = 333
Score = 114 bits (286), Expect = 1e-23
Identities = 66/114 (57%), Positives = 84/114 (73%), Gaps = 1/114 (0%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPMRYGLAAPLSGTTTATETVVPMYG 173
MAVEA HLNLFPPQ+LGNR MMN +E N+Y T M Y L PLSGTT+ TET++P+Y
Sbjct: 1 MAVEAGHLNLFPPQLLGNRE-MMNQVEANTNIYNTQMGYRL--PLSGTTSTTETLLPIYN 57
Query: 172 STITGTVPAKTAAIKSDSGLSY-AVPVSRKRSRDAINPLVSSSFPNGVQNQSAN 14
S IT + P KT IKS+SGLSY +P+ RKRSR++INPL+ S+P N++A+
Sbjct: 58 SVITDSFPQKT-PIKSESGLSYNHLPMQRKRSRESINPLL--SYPTPQPNKTAS 108
>gb|EOY13767.1| SBP family protein, putative [Theobroma cacao]
Length = 355
Score = 112 bits (280), Expect = 5e-23
Identities = 66/117 (56%), Positives = 82/117 (70%), Gaps = 2/117 (1%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPMRYGLAAPLSGTTT-ATETVVPMY 176
MAVEARHLNLF PQ LGNR MMN IE GN++ T M YG+ PLSGTTT ATE ++P Y
Sbjct: 1 MAVEARHLNLFRPQFLGNRE-MMNPIEANGNIFQTQMGYGV--PLSGTTTAATEGLLPFY 57
Query: 175 GSTITGTVPAKTAAIKSDSGLSY-AVPVSRKRSRDAINPLVSSSFPNGVQNQSANNR 8
S +T ++ K AAIKS+S L+Y +P+ RKRSRD+INP++S Q Q NN+
Sbjct: 58 NSVVTDSIHQKPAAIKSESTLTYNNLPLPRKRSRDSINPILSFPSLQQQQQQPHNNK 114
>gb|ABK96244.1| unknown [Populus trichocarpa x Populus deltoides]
Length = 340
Score = 109 bits (273), Expect = 3e-22
Identities = 64/111 (57%), Positives = 80/111 (72%), Gaps = 2/111 (1%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPMRYGLAAPLSG--TTTATETVVPM 179
MAVEARHLNLFPPQ++ +R + N IE N+Y T M YG+ PLSG TTT +T++PM
Sbjct: 1 MAVEARHLNLFPPQLISSRDIT-NPIEANTNMYNTQMGYGV--PLSGGTTTTTADTLLPM 57
Query: 178 YGSTITGTVPAKTAAIKSDSGLSYAVPVSRKRSRDAINPLVSSSFPNGVQN 26
Y S IT ++ KT IKSDSGL+Y VPV RKR RD+INPL+ S+P VQ+
Sbjct: 58 YSSVITDSISHKTP-IKSDSGLTYNVPVQRKRPRDSINPLL--SYPTLVQS 105
>ref|XP_002317616.1| hypothetical protein POPTR_0011s14590g [Populus trichocarpa]
gi|222860681|gb|EEE98228.1| hypothetical protein
POPTR_0011s14590g [Populus trichocarpa]
Length = 340
Score = 109 bits (272), Expect = 4e-22
Identities = 64/111 (57%), Positives = 80/111 (72%), Gaps = 2/111 (1%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPMRYGLAAPLSG--TTTATETVVPM 179
MAVEARHLNLFPPQ++ +R + N IE N+Y T M YG+ PLSG TTT +T++PM
Sbjct: 1 MAVEARHLNLFPPQLISSREIT-NPIEANTNMYNTQMGYGV--PLSGGTTTTTADTLLPM 57
Query: 178 YGSTITGTVPAKTAAIKSDSGLSYAVPVSRKRSRDAINPLVSSSFPNGVQN 26
Y S IT ++ KT IKSDSGL+Y VPV RKR RD+INPL+ S+P VQ+
Sbjct: 58 YSSVITDSISHKTP-IKSDSGLTYNVPVQRKRPRDSINPLL--SYPTLVQS 105
>gb|EMJ12699.1| hypothetical protein PRUPE_ppa007884mg [Prunus persica]
Length = 352
Score = 99.0 bits (245), Expect = 6e-19
Identities = 62/123 (50%), Positives = 79/123 (64%), Gaps = 6/123 (4%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPMRYGLAAPLSGTTTATETVVPMYG 173
MAVEARHLNLFP Q++ NR MMN I+G ++Y + YG+ PLS ATE ++P Y
Sbjct: 1 MAVEARHLNLFPSQLIPNRE-MMNPIQGNTSLYSAQIGYGV--PLSTGGVATEALLPTYN 57
Query: 172 STITGTV-PAK---TAAIKSDSGLSYAVPVS--RKRSRDAINPLVSSSFPNGVQNQSANN 11
S IT ++ P K TAA+KSDSGL+Y VP+S RKRSRD+IN +S F + N
Sbjct: 58 SVITDSIMPPKTSTTAAMKSDSGLTYNVPISLPRKRSRDSINNNISPPFSYPTAPANYKN 117
Query: 10 RCG 2
CG
Sbjct: 118 SCG 120
>ref|XP_004171715.1| PREDICTED: uncharacterized LOC101212862, partial [Cucumis sativus]
Length = 148
Score = 91.7 bits (226), Expect = 9e-17
Identities = 55/122 (45%), Positives = 78/122 (63%), Gaps = 8/122 (6%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVY-GTPMRYGLAAPLSGTTTATETVVPMY 176
MAVEARHLNLF PQ++G ++N IEG N+Y T M YG +SGTT+ ET++P Y
Sbjct: 1 MAVEARHLNLFHPQLIGGNRELVNLIEGDANMYNSTGMGYGGVPLVSGTTSTAETLLPAY 60
Query: 175 GSTIT-GTVPAKTAAI----KSDSGL--SYAVPVSRKRSRDAINPLVSSSFPNGVQNQSA 17
S I +V KTAA+ KSDSGL +Y +P+ RKR+R+ +N +S+P+ ++S
Sbjct: 61 NSVIVDSSVSPKTAAVSAAMKSDSGLTYNYTLPLPRKRARECMNINPFASYPSAPTSKSC 120
Query: 16 NN 11
+
Sbjct: 121 GS 122
>ref|XP_004139826.1| PREDICTED: uncharacterized protein LOC101212862 [Cucumis sativus]
Length = 351
Score = 91.7 bits (226), Expect = 9e-17
Identities = 55/122 (45%), Positives = 78/122 (63%), Gaps = 8/122 (6%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVY-GTPMRYGLAAPLSGTTTATETVVPMY 176
MAVEARHLNLF PQ++G ++N IEG N+Y T M YG +SGTT+ ET++P Y
Sbjct: 1 MAVEARHLNLFHPQLIGGNRELVNLIEGDANMYNSTGMGYGGVPLVSGTTSTAETLLPAY 60
Query: 175 GSTIT-GTVPAKTAAI----KSDSGL--SYAVPVSRKRSRDAINPLVSSSFPNGVQNQSA 17
S I +V KTAA+ KSDSGL +Y +P+ RKR+R+ +N +S+P+ ++S
Sbjct: 61 NSVIVDSSVSPKTAAVSAAMKSDSGLTYNYTLPLPRKRARECMNINPFASYPSAPTSKSC 120
Query: 16 NN 11
+
Sbjct: 121 GS 122
>gb|EXB53388.1| Baculoviral IAP repeat-containing protein 7-A [Morus notabilis]
Length = 361
Score = 90.1 bits (222), Expect = 3e-16
Identities = 61/125 (48%), Positives = 74/125 (59%), Gaps = 9/125 (7%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPMR------YGLAAPLSGTTTATET 191
MAVEARHLNLFPPQ++GNR + MN IE N++ YGL P +G A E
Sbjct: 1 MAVEARHLNLFPPQLIGNREI-MNPIETNANIFTNAHHQSNLGLYGL--PPAG-AIAAEA 56
Query: 190 VVPMYGSTITGTVPAKTAAIKSDSGLSYAV---PVSRKRSRDAINPLVSSSFPNGVQNQS 20
++P Y S I P KTA SDSGL+Y V P SRKRSRD+INPL+ S+P Q+
Sbjct: 57 LLPSYNSPIND--PPKTAKKSSDSGLTYTVNPTPYSRKRSRDSINPLL--SYPISTTAQT 112
Query: 19 ANNRC 5
N C
Sbjct: 113 MNKNC 117
>ref|XP_004488822.1| PREDICTED: uncharacterized protein LOC101497466 [Cicer arietinum]
Length = 368
Score = 87.8 bits (216), Expect = 1e-15
Identities = 62/114 (54%), Positives = 73/114 (64%), Gaps = 3/114 (2%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYG--TPMRYGLAAPLSGTTTATET-VVP 182
MAVEARHL+LF PQIL NR MMN E N++ + M Y P SGTTTATET ++P
Sbjct: 1 MAVEARHLHLFSPQILNNRE-MMNPNETNVNIFNNMSQMGYSSILPSSGTTTATETLILP 59
Query: 181 MYGSTITGTVPAKTAAIKSDSGLSYAVPVSRKRSRDAINPLVSSSFPNGVQNQS 20
Y S ++P KT A+ SDSGL+Y VP SRKRSRD N SS+FP N S
Sbjct: 60 AYNSITADSLPQKT-AMNSDSGLTYNVP-SRKRSRDN-NYSNSSNFPYPSYNLS 110
>ref|XP_004294111.1| PREDICTED: uncharacterized protein LOC101297222 [Fragaria vesca
subsp. vesca]
Length = 339
Score = 82.0 bits (201), Expect = 7e-14
Identities = 50/105 (47%), Positives = 69/105 (65%), Gaps = 8/105 (7%)
Frame = -3
Query: 352 MAVEARHLNLFPPQ-ILGNRGLMMNSIEGIGNVYGTPMRYGLAAPLSGTTTATETVVPMY 176
MAVEARHLNLFP Q + N +MN ++G G +YG + YG+ ++G ATE ++P Y
Sbjct: 1 MAVEARHLNLFPSQQLFCNNSEIMNPVQGNGGMYGAQIGYGVPM-MTGGLVATEALLPAY 59
Query: 175 G--STITGTVPAKTAAIKSDSGLSYAVPV----SRKRSR-DAINP 62
S + ++ KT A++SDSGL+Y VP+ SRKRSR D+INP
Sbjct: 60 NNNSMMNDSMLPKTTAMQSDSGLTYNVPISTSLSRKRSRVDSINP 104
>gb|AFK43980.1| unknown [Medicago truncatula]
Length = 291
Score = 79.0 bits (193), Expect = 6e-13
Identities = 52/108 (48%), Positives = 68/108 (62%), Gaps = 4/108 (3%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYG---TPMRYGLAAPLSGTTTATETVV- 185
MAVEA HL+LF PQ++ NR MMN +E N+ + M Y P + TTATET++
Sbjct: 1 MAVEAHHLHLFSPQLITNRE-MMNPVETNTNIIYNNISQMGYSSIPPSTIKTTATETMIL 59
Query: 184 PMYGSTITGTVPAKTAAIKSDSGLSYAVPVSRKRSRDAINPLVSSSFP 41
P Y S T ++P KTA + SDSGL+Y VP RKRSRD+ + S +FP
Sbjct: 60 PPYNSITTDSLPQKTA-MNSDSGLTYNVPPLRKRSRDSRDYSNSINFP 106
>gb|ACJ85143.1| unknown [Medicago truncatula]
Length = 199
Score = 79.0 bits (193), Expect = 6e-13
Identities = 52/108 (48%), Positives = 68/108 (62%), Gaps = 4/108 (3%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYG---TPMRYGLAAPLSGTTTATETVV- 185
MAVEA HL+LF PQ++ NR MMN +E N+ + M Y P + TTATET++
Sbjct: 1 MAVEAHHLHLFSPQLITNRE-MMNPVETNTNIIYNNISQMGYSSIPPSTIKTTATETMIL 59
Query: 184 PMYGSTITGTVPAKTAAIKSDSGLSYAVPVSRKRSRDAINPLVSSSFP 41
P Y S T ++P KTA + SDSGL+Y VP RKRSRD+ + S +FP
Sbjct: 60 PPYNSITTDSLPQKTA-MNSDSGLTYNVPPLRKRSRDSRDYSNSINFP 106
>ref|XP_003596191.1| S-RNase binding protein [Medicago truncatula]
gi|355485239|gb|AES66442.1| S-RNase binding protein
[Medicago truncatula]
Length = 366
Score = 79.0 bits (193), Expect = 6e-13
Identities = 52/108 (48%), Positives = 68/108 (62%), Gaps = 4/108 (3%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYG---TPMRYGLAAPLSGTTTATETVV- 185
MAVEA HL+LF PQ++ NR MMN +E N+ + M Y P + TTATET++
Sbjct: 1 MAVEAHHLHLFSPQLITNRE-MMNPVETNTNIIYNNISQMGYSSIPPSTIKTTATETMIL 59
Query: 184 PMYGSTITGTVPAKTAAIKSDSGLSYAVPVSRKRSRDAINPLVSSSFP 41
P Y S T ++P KTA + SDSGL+Y VP RKRSRD+ + S +FP
Sbjct: 60 PPYNSITTDSLPQKTA-MNSDSGLTYNVPPLRKRSRDSRDYSNSINFP 106
>gb|EOY27519.1| SBP family protein, putative isoform 2, partial [Theobroma cacao]
Length = 327
Score = 77.4 bits (189), Expect = 2e-12
Identities = 42/96 (43%), Positives = 57/96 (59%), Gaps = 2/96 (2%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPMRYGLAAPLSGTTTATETVVPMYG 173
MAVEARH+NLFPPQ++ N + + +G GN+Y T M PL + P+Y
Sbjct: 1 MAVEARHMNLFPPQLITNSSDFIKTNQGSGNIYNTQM--DSCVPLVDAMQEASQLFPLYQ 58
Query: 172 STITGTVPAKTAAIKSDSGLSYAVPVS--RKRSRDA 71
S + AKT+ K+DSGL+Y +PVS RKR RD+
Sbjct: 59 SLVCDPTSAKTSINKADSGLTYNIPVSAPRKRPRDS 94
>gb|EOY27518.1| SBP family protein, putative isoform 1 [Theobroma cacao]
Length = 328
Score = 74.7 bits (182), Expect = 1e-11
Identities = 42/96 (43%), Positives = 57/96 (59%), Gaps = 2/96 (2%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPMRYGLAAPLSGTTTATETVVPMYG 173
MAVEARH+NLFPPQ++ N + + +G GN+Y T M PL + P+Y
Sbjct: 1 MAVEARHMNLFPPQLITNSDFIKTN-QGSGNIYNTQM--DSCVPLVDAMQEASQLFPLYQ 57
Query: 172 STITGTVPAKTAAIKSDSGLSYAVPVS--RKRSRDA 71
S + AKT+ K+DSGL+Y +PVS RKR RD+
Sbjct: 58 SLVCDPTSAKTSINKADSGLTYNIPVSAPRKRPRDS 93
>ref|XP_006441059.1| hypothetical protein CICLE_v10021015mg [Citrus clementina]
gi|557543321|gb|ESR54299.1| hypothetical protein
CICLE_v10021015mg [Citrus clementina]
Length = 338
Score = 70.1 bits (170), Expect = 3e-10
Identities = 52/120 (43%), Positives = 69/120 (57%), Gaps = 17/120 (14%)
Frame = -3
Query: 352 MAVEARH-LNLFPPQILGNRGLMMNSIEGIGNVYGTPM----RYGLAAPLSGTTTATETV 188
MAVEARH ++FPPQ+L NR ++MN IE N+Y T M YG PLSGT T E
Sbjct: 1 MAVEARHQYSIFPPQLLANREIIMNPIEANSNIYNTQMGGTGGYG-RLPLSGTATTAEAF 59
Query: 187 VP--MYGSTI-TGTVPAKTAAI-KSDSGLSY--------AVPVSRKRSRDAINPLVSSSF 44
+P +YGS+I + + P + I KSDS L+Y + SRKRSR++ + SF
Sbjct: 60 LPTVVYGSSINSDSFPHQKPLINKSDSSLTYNNYENNNLPLTPSRKRSRESCSTPTPFSF 119
>ref|XP_006478050.1| PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 3-like
[Citrus sinensis]
Length = 340
Score = 68.9 bits (167), Expect = 7e-10
Identities = 51/120 (42%), Positives = 69/120 (57%), Gaps = 17/120 (14%)
Frame = -3
Query: 352 MAVEARH-LNLFPPQILGNRGLMMNSIEGIGNVYGTPM----RYGLAAPLSGTTTATETV 188
MAVEARH ++FPPQ+L NR +++N IE N+Y T M YG PLSGT T E
Sbjct: 1 MAVEARHQYSIFPPQLLANREIILNPIEANSNIYNTQMGGTGGYG-RLPLSGTATTAEAF 59
Query: 187 VP--MYGSTI-TGTVPAKTAAI-KSDSGLSY--------AVPVSRKRSRDAINPLVSSSF 44
+P +YGS+I + + P + I KSDS L+Y + SRKRSR++ + SF
Sbjct: 60 LPTVVYGSSINSDSFPHQKPLINKSDSSLTYNNYENNNLPLTPSRKRSRESCSTPTPFSF 119
>ref|XP_002266864.2| PREDICTED: uncharacterized protein LOC100255881 [Vitis vinifera]
Length = 313
Score = 68.2 bits (165), Expect = 1e-09
Identities = 44/105 (41%), Positives = 60/105 (57%), Gaps = 1/105 (0%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPM-RYGLAAPLSGTTTATETVVPMY 176
MAVEAR +NLFP Q + N+ +M S++G G VYG+ + + GL + +VP Y
Sbjct: 1 MAVEARRINLFPSQFIPNKRQVMGSLDGNGVVYGSQVGQRGL--------VSENLMVPFY 52
Query: 175 GSTITGTVPAKTAAIKSDSGLSYAVPVSRKRSRDAINPLVSSSFP 41
S PA K+DSGL+Y +PV+RKR+RD V SFP
Sbjct: 53 SSAFVDPAPA-----KADSGLTYNLPVARKRARD---ESVMMSFP 89
>emb|CBI21767.3| unnamed protein product [Vitis vinifera]
Length = 289
Score = 68.2 bits (165), Expect = 1e-09
Identities = 44/105 (41%), Positives = 60/105 (57%), Gaps = 1/105 (0%)
Frame = -3
Query: 352 MAVEARHLNLFPPQILGNRGLMMNSIEGIGNVYGTPM-RYGLAAPLSGTTTATETVVPMY 176
MAVEAR +NLFP Q + N+ +M S++G G VYG+ + + GL + +VP Y
Sbjct: 1 MAVEARRINLFPSQFIPNKRQVMGSLDGNGVVYGSQVGQRGL--------VSENLMVPFY 52
Query: 175 GSTITGTVPAKTAAIKSDSGLSYAVPVSRKRSRDAINPLVSSSFP 41
S PA K+DSGL+Y +PV+RKR+RD V SFP
Sbjct: 53 SSAFVDPAPA-----KADSGLTYNLPVARKRARD---ESVMMSFP 89