BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia24_contig00022990
         (1128 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_006472717.1| PREDICTED: biotin--protein ligase-like [Citr...   488   e-135
ref|XP_006434124.1| hypothetical protein CICLE_v10001583mg [Citr...   487   e-135
ref|XP_002301010.2| hypothetical protein POPTR_0002s08830g [Popu...   485   e-134
ref|XP_003523464.1| PREDICTED: biotin--protein ligase [Glycine max]   485   e-134
ref|XP_004290831.1| PREDICTED: biotin--protein ligase-like [Frag...   480   e-133
gb|EMJ22979.1| hypothetical protein PRUPE_ppa007504mg [Prunus pe...   479   e-132
gb|EOY16190.1| Holocarboxylase synthase 1 isoform 1 [Theobroma c...   474   e-131
ref|XP_003526610.1| PREDICTED: biotin--protein ligase-like isofo...   474   e-131
ref|XP_004500594.1| PREDICTED: biotin--protein ligase-like [Cice...   473   e-131
ref|XP_003601098.1| Holocarboxylase synthetase hcs2 [Medicago tr...   471   e-130
gb|ESW09361.1| hypothetical protein PHAVU_009G121400g [Phaseolus...   470   e-130
ref|XP_002307419.1| holocarboxylase synthetase 2 family protein ...   470   e-130
emb|CBI19072.3| unnamed protein product [Vitis vinifera]              468   e-129
ref|XP_002285834.1| PREDICTED: biotin--protein ligase [Vitis vin...   468   e-129
ref|XP_004250339.1| PREDICTED: biotin--protein ligase-like [Sola...   464   e-128
ref|XP_006351872.1| PREDICTED: biotin--protein ligase-like [Sola...   462   e-128
ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis ly...   450   e-124
gb|AAL93108.1|AF414937_1 holocarboxylase synthetase 1 [Arabidops...   449   e-124
ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thalian...   449   e-123
ref|XP_002878877.1| holocarboxylase synthetase 1 [Arabidopsis ly...   444   e-122

>ref|XP_006472717.1| PREDICTED: biotin--protein ligase-like [Citrus sinensis]
          Length = 366

 Score =  488 bits (1256), Expect = e-135
 Identities = 238/334 (71%), Positives = 284/334 (85%)
 Frame = +1

Query: 127  SAKMEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDD 306
            SA M+  SS +LV+ GKS AENE+A  LK+N+TLKLPD+S V++ LQSEI  QE+     
Sbjct: 39   SAAMDSNSSCVLVLSGKSLAENEIAESLKSNSTLKLPDNSEVSIHLQSEIVKQES----- 93

Query: 307  IFRINSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKN 486
             F +  +++ L+T+RFG+LLI+SP+L STHDVVS NF ELP+G VCVADVQFKGRGRSKN
Sbjct: 94   -FDVELFMNSLATNRFGRLLIWSPRLPSTHDVVSHNFCELPVGAVCVADVQFKGRGRSKN 152

Query: 487  VWESPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDL 666
             WESPKGCL+FSFTIQMEDGR+VPL+QYV SLA+TEAI DVC R+G+P LD++IKWPNDL
Sbjct: 153  AWESPKGCLMFSFTIQMEDGRVVPLLQYVASLALTEAIKDVCSRDGLPCLDIKIKWPNDL 212

Query: 667  YLDGLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEII 846
            YL+G+KVGGILCTSTY++KKFNVS GIGLNVNN++PT CLNAVL++L     Q QRE++I
Sbjct: 213  YLNGIKVGGILCTSTYRTKKFNVSIGIGLNVNNEEPTTCLNAVLRKLSDSTYQFQREDVI 272

Query: 847  AAFFNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLT 1026
            AAFFNKFE FYD FI QGFQ+LEELYYKTWLHSGQRVI+QEKNE  DQ+ E+VVTI+GLT
Sbjct: 273  AAFFNKFETFYDTFINQGFQSLEELYYKTWLHSGQRVIIQEKNE--DQVVENVVTIQGLT 330

Query: 1027 TSGYLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            +SGYLLA  +D Q CELHPDGNS DFFKGL+RRK
Sbjct: 331  SSGYLLAMGDDNQMCELHPDGNSLDFFKGLIRRK 364


>ref|XP_006434124.1| hypothetical protein CICLE_v10001583mg [Citrus clementina]
            gi|557536246|gb|ESR47364.1| hypothetical protein
            CICLE_v10001583mg [Citrus clementina]
          Length = 366

 Score =  487 bits (1253), Expect = e-135
 Identities = 239/334 (71%), Positives = 283/334 (84%)
 Frame = +1

Query: 127  SAKMEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDD 306
            SA M+  SS MLV+ GKS AENE+A  LK+N+TLKLPD+S V++ LQSEI  QE+     
Sbjct: 39   SAAMDSNSSCMLVLSGKSLAENEIAESLKSNSTLKLPDNSEVSIHLQSEIVKQES----- 93

Query: 307  IFRINSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKN 486
             F +  +++ L+T+RFG+LLI+SP+L STHDVVS NF ELP+G VCVADVQFKGRGRSKN
Sbjct: 94   -FDVELFMNSLATNRFGRLLIWSPRLPSTHDVVSHNFCELPVGAVCVADVQFKGRGRSKN 152

Query: 487  VWESPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDL 666
             WESPKGCL+FSFTIQMEDGR+VPL+QYV SLA+TEAIN VC R+G+P LD++IKWPNDL
Sbjct: 153  AWESPKGCLMFSFTIQMEDGRVVPLLQYVASLALTEAINYVCSRDGLPCLDIKIKWPNDL 212

Query: 667  YLDGLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEII 846
            YL+G+KVGGILCTSTY++KKFNVS GIGLNVNN++PT CLNAVL++L     Q +RE++I
Sbjct: 213  YLNGIKVGGILCTSTYRTKKFNVSIGIGLNVNNEEPTTCLNAVLRKLSDSTYQFRREDVI 272

Query: 847  AAFFNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLT 1026
            AAFFNKFE FYD FI QGFQ LEELYYKTWLHSGQRVIVQEKNE  DQ+ E+VVTI+GLT
Sbjct: 273  AAFFNKFETFYDTFINQGFQTLEELYYKTWLHSGQRVIVQEKNE--DQVVENVVTIQGLT 330

Query: 1027 TSGYLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            +SGYLLA  +D Q CELHPDGNS DFFKGL+RRK
Sbjct: 331  SSGYLLAIGDDNQMCELHPDGNSLDFFKGLIRRK 364


>ref|XP_002301010.2| hypothetical protein POPTR_0002s08830g [Populus trichocarpa]
            gi|550344587|gb|EEE80283.2| hypothetical protein
            POPTR_0002s08830g [Populus trichocarpa]
          Length = 390

 Score =  485 bits (1249), Expect = e-134
 Identities = 244/349 (69%), Positives = 293/349 (83%), Gaps = 3/349 (0%)
 Frame = +1

Query: 91   QKFSFTPNKPYLSAK-MEPQSST--MLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVV 261
            QK  FT ++   +A  M+  SST   LV+CGKS+AENE+A+ L NNNTLKLPD+  V+ +
Sbjct: 42   QKSPFTLSRSVAAATAMDSSSSTPCKLVLCGKSSAENEIAKSLMNNNTLKLPDNVQVSTL 101

Query: 262  LQSEIESQENVGNDDIFRINSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTV 441
            L SEI +++   +++ F I  +++ LST++FG+LLI+SP L STHDVVS+NF ELPIGTV
Sbjct: 102  LHSEIINKQQRQDEESFCIERFMNSLSTNQFGRLLIWSPILPSTHDVVSNNFGELPIGTV 161

Query: 442  CVADVQFKGRGRSKNVWESPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRN 621
            CVADVQ+KGRGRSKNVWESP GCL+FSFTIQMEDGR+VPL+QYVVSLA+TEAI DVC +N
Sbjct: 162  CVADVQYKGRGRSKNVWESPAGCLMFSFTIQMEDGRVVPLLQYVVSLAVTEAIKDVCDKN 221

Query: 622  GMPSLDVRIKWPNDLYLDGLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQ 801
            G+P +DVRIKWPNDLYL+GLKVGGIL TSTYKSKKFNVS GIGLNV+N+KPT CLNAVL+
Sbjct: 222  GLPRIDVRIKWPNDLYLNGLKVGGILSTSTYKSKKFNVSTGIGLNVDNEKPTTCLNAVLR 281

Query: 802  RLGCVAPQVQREEIIAAFFNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNED 981
             L   A  ++RE+I+AAF NKFE FYD+FI  GF+ LEELYYKTWLHSGQRVI+QEKNE 
Sbjct: 282  ELSAAACTLRREDIVAAFLNKFENFYDLFINGGFRTLEELYYKTWLHSGQRVIIQEKNE- 340

Query: 982  QDQISEHVVTIKGLTTSGYLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
             +Q+ E+VVTI+GLT+SGYLLA  ED Q CELHPDGNSFDFFKGLVRRK
Sbjct: 341  -NQVVENVVTIQGLTSSGYLLAIGEDNQMCELHPDGNSFDFFKGLVRRK 388


>ref|XP_003523464.1| PREDICTED: biotin--protein ligase [Glycine max]
          Length = 361

 Score =  485 bits (1248), Expect = e-134
 Identities = 235/325 (72%), Positives = 278/325 (85%)
 Frame = +1

Query: 154  TMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDDIFRINSYVD 333
            ++LV+CGKS AENE A+ +K NNTLKLP+D  +++VL SE++     G    F+++SY+D
Sbjct: 40   SLLVLCGKSLAENETAKAIKTNNTLKLPEDEKLSLVLHSEMDKSVMQG---FFQVHSYMD 96

Query: 334  LLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKNVWESPKGCL 513
             LST++FG+LL++SP L STHDVVS NF ELPIGTVC+ADVQ KGRGRSKNVWESP GCL
Sbjct: 97   SLSTNQFGRLLVWSPDLTSTHDVVSHNFCELPIGTVCIADVQTKGRGRSKNVWESPLGCL 156

Query: 514  LFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDLYLDGLKVGG 693
            +FSFT+QMEDGR+VPLVQYVVSLAMTEAI D+C +NG+PS+DV+IKWPNDLYL+G KVGG
Sbjct: 157  MFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDICDKNGLPSIDVKIKWPNDLYLNGSKVGG 216

Query: 694  ILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEIIAAFFNKFEC 873
            ILCTSTYKSKKFNVSAGIGLNVNN+KPT  LN +L+     A Q QREE++AAFFNKFE 
Sbjct: 217  ILCTSTYKSKKFNVSAGIGLNVNNKKPTTSLNTILKGFYSGAYQFQREEVLAAFFNKFEI 276

Query: 874  FYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLTTSGYLLAAT 1053
            FYD+F+ QGFQ LEELY KTWLHSGQRV+VQEKNE  D++ EHVVTI+GLT+SGYLLA  
Sbjct: 277  FYDLFLNQGFQTLEELYIKTWLHSGQRVVVQEKNE--DKVIEHVVTIQGLTSSGYLLAVG 334

Query: 1054 EDGQTCELHPDGNSFDFFKGLVRRK 1128
            +D Q CELHPDGNSFDFFKGLVRRK
Sbjct: 335  DDHQMCELHPDGNSFDFFKGLVRRK 359


>ref|XP_004290831.1| PREDICTED: biotin--protein ligase-like [Fragaria vesca subsp. vesca]
          Length = 329

 Score =  480 bits (1235), Expect = e-133
 Identities = 235/331 (70%), Positives = 278/331 (83%)
 Frame = +1

Query: 136  MEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDDIFR 315
            M+  SS MLV+ GKS AEN++A  LKN   LKL DD+ ++++L SE E     G  + F 
Sbjct: 1    MDCNSSCMLVLRGKSPAENKIAEALKNTKALKLSDDTELSILLDSESEKPFKEG--EAFG 58

Query: 316  INSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKNVWE 495
            ++S++  LST RFG+ L++SP+L ST DVVS NF ELP G VCVADVQFKGRGRSKNVWE
Sbjct: 59   VDSFMKSLSTDRFGRFLLWSPRLPSTQDVVSQNFCELPNGAVCVADVQFKGRGRSKNVWE 118

Query: 496  SPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDLYLD 675
            SPKGCLLFSFT+QMEDGR+VPL+QYVVSLA+TEAI DVC RNG+P +DVRIKWPNDLYL+
Sbjct: 119  SPKGCLLFSFTLQMEDGRVVPLIQYVVSLAITEAIKDVCDRNGLPYIDVRIKWPNDLYLN 178

Query: 676  GLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEIIAAF 855
            GLKVGGILCTSTYKSKKFNVSAG+GLN+NN+KPT CLNAVL+ L     Q +REEI+AAF
Sbjct: 179  GLKVGGILCTSTYKSKKFNVSAGVGLNINNEKPTTCLNAVLRELSATTYQFRREEILAAF 238

Query: 856  FNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLTTSG 1035
            FNKFE FYD+F+ QGFQ+LEELYYKTWLHSGQRVIVQEKN+  DQ+ E+VVT++GLT++G
Sbjct: 239  FNKFEKFYDLFMHQGFQSLEELYYKTWLHSGQRVIVQEKND--DQVVENVVTVQGLTSAG 296

Query: 1036 YLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            YLLA  +D Q CELHPDGNSFDFFKGLVR+K
Sbjct: 297  YLLAIGDDNQMCELHPDGNSFDFFKGLVRQK 327


>gb|EMJ22979.1| hypothetical protein PRUPE_ppa007504mg [Prunus persica]
          Length = 365

 Score =  479 bits (1232), Expect = e-132
 Identities = 234/349 (67%), Positives = 293/349 (83%)
 Frame = +1

Query: 82   LSLQKFSFTPNKPYLSAKMEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVV 261
            LS +K + +   P+  + M+  SS  LV+CGKS+AENE A+ LKN+  LKL D++ ++++
Sbjct: 20   LSTKKTNSSLPLPFAVSAMDGNSSCALVLCGKSSAENETAKTLKNSGALKLTDNTELSIL 79

Query: 262  LQSEIESQENVGNDDIFRINSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTV 441
            L SE  S + +  +D F ++S+++ LST+RFG+ L++SP+L STHDV+S NF ELPIG V
Sbjct: 80   LHSE--SAKPI-KEDAFGVDSFMNSLSTNRFGRFLLWSPRLPSTHDVISQNFCELPIGAV 136

Query: 442  CVADVQFKGRGRSKNVWESPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRN 621
            CVADVQ+KGRGRSKNVWESPKGCLLFSFT+QMEDGR+VPL+QYVVSLA+TEAI DVC +N
Sbjct: 137  CVADVQYKGRGRSKNVWESPKGCLLFSFTLQMEDGRVVPLIQYVVSLAVTEAIKDVCDKN 196

Query: 622  GMPSLDVRIKWPNDLYLDGLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQ 801
            G+P +DV+IKWPNDLYL+GLKVGGILCTST+KSK+FNVSAGIGLNV+N+KPT CLN  L+
Sbjct: 197  GLPYVDVKIKWPNDLYLNGLKVGGILCTSTFKSKQFNVSAGIGLNVDNEKPTTCLNTFLR 256

Query: 802  RLGCVAPQVQREEIIAAFFNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNED 981
             L     Q +RE+I+AAFF+KFE FY++FI QGFQ+LEELYYKTWLHSGQRVIVQEKN+ 
Sbjct: 257  ELSVTTYQFRREDILAAFFDKFEKFYELFINQGFQSLEELYYKTWLHSGQRVIVQEKND- 315

Query: 982  QDQISEHVVTIKGLTTSGYLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
             DQ+ E+VVTI+GLT+SGYLLA  +D Q CELHPDGNSFDFFKGLVR+K
Sbjct: 316  -DQVVENVVTIQGLTSSGYLLAIGDDNQMCELHPDGNSFDFFKGLVRQK 363


>gb|EOY16190.1| Holocarboxylase synthase 1 isoform 1 [Theobroma cacao]
          Length = 375

 Score =  474 bits (1220), Expect = e-131
 Identities = 237/355 (66%), Positives = 287/355 (80%), Gaps = 3/355 (0%)
 Frame = +1

Query: 73   LPFLSLQKFSFTPNKPYLS---AKMEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDD 243
            + F  L   + TP++   S   + ME  S + L +CGKS++E E+A+ LK NN LKLP+ 
Sbjct: 23   ISFSVLSLKNHTPSRLSFSVAASAMETSSPSALFLCGKSSSETEIAKSLKINNALKLPNG 82

Query: 244  SPVTVVLQSEIESQENVGNDDIFRINSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFME 423
              V+  LQSEI + +    ++ F +N +++ LST+RFG+ LI+SP+L STHDVVS NF E
Sbjct: 83   GEVSTHLQSEISNFQL--GEEAFNVNLFLNSLSTNRFGRNLIWSPRLPSTHDVVSHNFCE 140

Query: 424  LPIGTVCVADVQFKGRGRSKNVWESPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAIN 603
            LPIG  CVADVQFKGRGRSKN WESP+GCL+FSFT++MEDG+IVPLVQYVVSLA+TEAI 
Sbjct: 141  LPIGAACVADVQFKGRGRSKNAWESPRGCLMFSFTLEMEDGKIVPLVQYVVSLALTEAIK 200

Query: 604  DVCRRNGMPSLDVRIKWPNDLYLDGLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTIC 783
            D+C R G P LDVRIKWPNDLYL+GLKVGGILCTSTYKSKKFNVS+G+GLNV+N+KPT C
Sbjct: 201  DICDREGFPCLDVRIKWPNDLYLNGLKVGGILCTSTYKSKKFNVSSGVGLNVDNEKPTTC 260

Query: 784  LNAVLQRLGCVAPQVQREEIIAAFFNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIV 963
            LNAVL+ L   + + +RE+I+AAFFNKFE  YDIF+ QGFQ LEELYY+TWLHSGQRVIV
Sbjct: 261  LNAVLKELSTTSNKFRREDIMAAFFNKFEMLYDIFLNQGFQTLEELYYRTWLHSGQRVIV 320

Query: 964  QEKNEDQDQISEHVVTIKGLTTSGYLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            QEKNE  D+I E+VVTI+GLT+SGYLLA  +D Q CELHPDGNSFDFFKGLVRRK
Sbjct: 321  QEKNE--DRIVENVVTIQGLTSSGYLLAIGDDYQMCELHPDGNSFDFFKGLVRRK 373


>ref|XP_003526610.1| PREDICTED: biotin--protein ligase-like isoform X1 [Glycine max]
          Length = 361

 Score =  474 bits (1219), Expect = e-131
 Identities = 230/325 (70%), Positives = 276/325 (84%)
 Frame = +1

Query: 154  TMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDDIFRINSYVD 333
            ++LV+CGKS AENE A+ +K ++TLKLP+D  +++VL SE+++    G    F+++SY+D
Sbjct: 40   SLLVLCGKSLAENETAKAIKTSSTLKLPEDEKLSLVLHSEMDNSVMQG---FFQVHSYMD 96

Query: 334  LLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKNVWESPKGCL 513
             LST++FG+LLI+SP L STHD+VS NF ELPIGTV +ADVQ KGRGRSKNVWESP GCL
Sbjct: 97   SLSTNQFGRLLIWSPDLNSTHDIVSHNFCELPIGTVSIADVQTKGRGRSKNVWESPLGCL 156

Query: 514  LFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDLYLDGLKVGG 693
            +FSFT+QMEDGR+VPLVQYVVSLAMTEAI D+C +NG+PS+DVRIKWPNDLYL+G KVGG
Sbjct: 157  MFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDICDKNGLPSIDVRIKWPNDLYLNGCKVGG 216

Query: 694  ILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEIIAAFFNKFEC 873
            ILCTSTYKSKKFNVSAGIGLNVNN+ PT  LN +L+     A Q QRE+++AAFFNKFE 
Sbjct: 217  ILCTSTYKSKKFNVSAGIGLNVNNKNPTTSLNTILKGFYTGAYQFQREDVLAAFFNKFEK 276

Query: 874  FYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLTTSGYLLAAT 1053
            FYD+F+ QGFQ LEELY KTWLHSGQRV+VQEKNE  D++ EHVVTI+GLT+SGYLLA  
Sbjct: 277  FYDLFVNQGFQTLEELYIKTWLHSGQRVVVQEKNE--DKVIEHVVTIQGLTSSGYLLAVG 334

Query: 1054 EDGQTCELHPDGNSFDFFKGLVRRK 1128
            +D   CELHPDGNSFDFFKGLVRRK
Sbjct: 335  DDNHMCELHPDGNSFDFFKGLVRRK 359


>ref|XP_004500594.1| PREDICTED: biotin--protein ligase-like [Cicer arietinum]
          Length = 347

 Score =  473 bits (1216), Expect = e-131
 Identities = 227/323 (70%), Positives = 275/323 (85%)
 Frame = +1

Query: 160  LVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDDIFRINSYVDLL 339
            LV+CGKS AE E A  +K NNTLKLPD   +++VL SE+       +   F+++S+++ L
Sbjct: 28   LVLCGKSNAEIETAIAIKANNTLKLPDKGELSLVLHSELNKSVEQNS---FQVSSFMNSL 84

Query: 340  STSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKNVWESPKGCLLF 519
            ST++FG+LLI+SP+L STHDVVS+NF ELP+GTVCVAD+Q KGRGRSKNVWESP GCL+F
Sbjct: 85   STNQFGRLLIWSPRLTSTHDVVSNNFCELPLGTVCVADIQNKGRGRSKNVWESPLGCLMF 144

Query: 520  SFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDLYLDGLKVGGIL 699
            SFT+QMEDGR+VPLVQYVVSLA+TEAI D+C +NG+P +DV+IKWPNDLYL+G KVGGIL
Sbjct: 145  SFTLQMEDGRVVPLVQYVVSLAITEAIKDICDKNGLPCIDVKIKWPNDLYLNGFKVGGIL 204

Query: 700  CTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEIIAAFFNKFECFY 879
            CTSTYKSKKFN+SAGIGLNV+N+KPT  LN VL+ L   A Q QRE+++AAFFNKFE F+
Sbjct: 205  CTSTYKSKKFNISAGIGLNVHNEKPTTSLNTVLRELSVGAYQFQREDVLAAFFNKFEIFF 264

Query: 880  DIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLTTSGYLLAATED 1059
            D+F+ QGFQ LEELYYKTWLHSGQRV+VQEKNE  D++ EHVVT++GLT+SGYLLA  +D
Sbjct: 265  DLFVNQGFQTLEELYYKTWLHSGQRVVVQEKNE--DKVVEHVVTVQGLTSSGYLLAIGDD 322

Query: 1060 GQTCELHPDGNSFDFFKGLVRRK 1128
             Q CELHPDGNSFDFFKGLVRRK
Sbjct: 323  NQMCELHPDGNSFDFFKGLVRRK 345


>ref|XP_003601098.1| Holocarboxylase synthetase hcs2 [Medicago truncatula]
            gi|355490146|gb|AES71349.1| Holocarboxylase synthetase
            hcs2 [Medicago truncatula]
          Length = 361

 Score =  471 bits (1213), Expect = e-130
 Identities = 227/325 (69%), Positives = 274/325 (84%)
 Frame = +1

Query: 154  TMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDDIFRINSYVD 333
            ++LV+CGKS AENE A  +K NNTLKLPD   ++++L SE    ++V     F+ +S+++
Sbjct: 39   SLLVLCGKSLAENETAIAIKTNNTLKLPDKKELSIILHSEFN--KSVIQRQSFQFSSFMN 96

Query: 334  LLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKNVWESPKGCL 513
             LST++FG+LLI+SP+L STHDVVS NF ELP+GTVCV D+Q KGRGRSKN WESP GCL
Sbjct: 97   SLSTNQFGRLLIWSPELTSTHDVVSHNFCELPVGTVCVGDIQNKGRGRSKNAWESPLGCL 156

Query: 514  LFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDLYLDGLKVGG 693
            +FSFT+QMEDGRIVPLVQYVVSLA+TEAI D+C +NG+P +DV+IKWPNDLYL+G KVGG
Sbjct: 157  MFSFTLQMEDGRIVPLVQYVVSLAITEAIKDICDKNGLPCVDVKIKWPNDLYLNGFKVGG 216

Query: 694  ILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEIIAAFFNKFEC 873
            ILCTSTY+SKKFN+SAGIGLNVNN+KPT  LN VL+ L   A Q QRE+++AAFFNKFE 
Sbjct: 217  ILCTSTYRSKKFNISAGIGLNVNNEKPTTSLNTVLRELSVGAYQFQREDVLAAFFNKFEK 276

Query: 874  FYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLTTSGYLLAAT 1053
            F+D+F+ QGFQ LEELYYKTWLHSGQRVIVQEKNE  +++ EH VTI+GLT+SGYLLA  
Sbjct: 277  FFDLFLNQGFQTLEELYYKTWLHSGQRVIVQEKNE--EKVVEHAVTIQGLTSSGYLLAVG 334

Query: 1054 EDGQTCELHPDGNSFDFFKGLVRRK 1128
            +D Q CELHPDGNSFDFFKGLVRRK
Sbjct: 335  DDNQMCELHPDGNSFDFFKGLVRRK 359


>gb|ESW09361.1| hypothetical protein PHAVU_009G121400g [Phaseolus vulgaris]
          Length = 360

 Score =  470 bits (1209), Expect = e-130
 Identities = 229/325 (70%), Positives = 273/325 (84%)
 Frame = +1

Query: 154  TMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDDIFRINSYVD 333
            ++LV+ GKS AENE A+ +K NNTLKLP++   ++VL S+++     G+   F+I S++D
Sbjct: 39   SLLVLYGKSLAENETAKAIKTNNTLKLPENGEYSLVLHSQLDKALMQGS---FQIPSFMD 95

Query: 334  LLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKNVWESPKGCL 513
             LST +FG+LLI+SP+L STHD+VS NF ELPIGTVCVADVQ KGRGRSKNVWESP GCL
Sbjct: 96   SLSTKQFGRLLIWSPQLTSTHDIVSQNFCELPIGTVCVADVQTKGRGRSKNVWESPSGCL 155

Query: 514  LFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDLYLDGLKVGG 693
            +FSFT+QMEDGR+VPLVQYVVSLA+TEA+ D+C +NG+PS+D +IKWPNDLYL+G KVGG
Sbjct: 156  MFSFTLQMEDGRVVPLVQYVVSLAITEAVKDICYKNGLPSIDAKIKWPNDLYLNGSKVGG 215

Query: 694  ILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEIIAAFFNKFEC 873
            ILCTSTYKSKKFNVSAGIGLNVNN+KPT  LN VL+ L     Q QRE+I+AAFFNKFE 
Sbjct: 216  ILCTSTYKSKKFNVSAGIGLNVNNEKPTTSLNTVLRELSTGGYQFQREDILAAFFNKFEE 275

Query: 874  FYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLTTSGYLLAAT 1053
            FY +F+ QGFQ LEELY KTWLHSGQRV+VQEKN   D++ EHVVTI+GLT+SGYLLA  
Sbjct: 276  FYGLFVNQGFQTLEELYIKTWLHSGQRVVVQEKN--GDKVIEHVVTIQGLTSSGYLLAVG 333

Query: 1054 EDGQTCELHPDGNSFDFFKGLVRRK 1128
            +D Q CELHPDGNSFDFFKGLVRRK
Sbjct: 334  DDNQMCELHPDGNSFDFFKGLVRRK 358


>ref|XP_002307419.1| holocarboxylase synthetase 2 family protein [Populus trichocarpa]
            gi|222856868|gb|EEE94415.1| holocarboxylase synthetase 2
            family protein [Populus trichocarpa]
          Length = 331

 Score =  470 bits (1209), Expect = e-130
 Identities = 228/323 (70%), Positives = 277/323 (85%)
 Frame = +1

Query: 160  LVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDDIFRINSYVDLL 339
            L++CGKS+AENE+A+ L NNNTLKLPD+  ++ +  SEI   E   N++ F ++ +++ L
Sbjct: 10   LLLCGKSSAENEIAKSLMNNNTLKLPDNVEISTLSHSEI-LDEPQQNEESFSLSRFMNSL 68

Query: 340  STSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKNVWESPKGCLLF 519
            ST+ FG+LLI+SP L STHD+VS+NF ELPIGTVC+ADVQ+KGRGRSKNVWESP GCL+F
Sbjct: 69   STNLFGRLLIWSPLLPSTHDLVSNNFGELPIGTVCIADVQYKGRGRSKNVWESPAGCLMF 128

Query: 520  SFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDLYLDGLKVGGIL 699
            SFTIQMEDG++VPL+QYVVSLA+TEAI D+C +NG+P +DVRIKWPNDLYL+G+KVGGIL
Sbjct: 129  SFTIQMEDGQVVPLLQYVVSLAVTEAIKDLCDKNGLPHIDVRIKWPNDLYLNGVKVGGIL 188

Query: 700  CTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEIIAAFFNKFECFY 879
            CTSTY+SKKFN+SAGIGLNV+N+KPT CLNAVL+ L   A  ++RE+I+AAF NKFE  Y
Sbjct: 189  CTSTYRSKKFNISAGIGLNVDNEKPTTCLNAVLRELAGAACALRREDIVAAFLNKFEKLY 248

Query: 880  DIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLTTSGYLLAATED 1059
            D+FI  GFQ LEELYYKTWLHSGQRVI+QEKNE  +Q+ E+VVTI+GLT SGYLLA  ED
Sbjct: 249  DLFINGGFQTLEELYYKTWLHSGQRVIIQEKNE--NQVVENVVTIQGLTPSGYLLAIGED 306

Query: 1060 GQTCELHPDGNSFDFFKGLVRRK 1128
             Q CELHPDGNSFDFFKGLVRRK
Sbjct: 307  NQMCELHPDGNSFDFFKGLVRRK 329


>emb|CBI19072.3| unnamed protein product [Vitis vinifera]
          Length = 416

 Score =  468 bits (1205), Expect = e-129
 Identities = 232/334 (69%), Positives = 277/334 (82%)
 Frame = +1

Query: 127  SAKMEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDD 306
            S  M+ QS+++LV+CGKS+AE E A+ LK  NTLKLPD+  V+++L  EIE  E    ++
Sbjct: 87   SLAMDSQSTSLLVLCGKSSAEIEFAKSLKAKNTLKLPDNGEVSILLHPEIEKPE----EE 142

Query: 307  IFRINSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKN 486
             F+++ ++  LST+ FG+ LI+SP+L STHDVVS NF ELPIGTVCVADVQ KGRGRSKN
Sbjct: 143  PFQMDLFMSSLSTNTFGRFLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSKN 202

Query: 487  VWESPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDL 666
             WESP+GCLLFSFT+QMEDGR VPL+QYVVSLA+TEAI  +C   G+P LDV+IKWPNDL
Sbjct: 203  KWESPRGCLLFSFTLQMEDGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPNDL 262

Query: 667  YLDGLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEII 846
            YL+GLKVGGILCTSTYKS+KFNVSAGIGLNV+N+KPT CLNAVL+ L  VA Q +RE+I+
Sbjct: 263  YLNGLKVGGILCTSTYKSRKFNVSAGIGLNVDNEKPTTCLNAVLRELSSVAYQFRREDIV 322

Query: 847  AAFFNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLT 1026
            AAFFNKFE F+ +FI QGFQ LEELY KTWLHSGQRVIVQE  E   Q+ E+VVTI+GL 
Sbjct: 323  AAFFNKFETFFSLFINQGFQTLEELYCKTWLHSGQRVIVQENIE--GQMVENVVTIQGLA 380

Query: 1027 TSGYLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            +SGYLLA  +DGQ CELHPDGNSFDFFKGL+RRK
Sbjct: 381  SSGYLLAIGDDGQMCELHPDGNSFDFFKGLIRRK 414


>ref|XP_002285834.1| PREDICTED: biotin--protein ligase [Vitis vinifera]
          Length = 327

 Score =  468 bits (1203), Expect = e-129
 Identities = 231/331 (69%), Positives = 276/331 (83%)
 Frame = +1

Query: 136  MEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDDIFR 315
            M+ QS+++LV+CGKS+AE E A+ LK  NTLKLPD+  V+++L  EIE  E    ++ F+
Sbjct: 1    MDSQSTSLLVLCGKSSAEIEFAKSLKAKNTLKLPDNGEVSILLHPEIEKPE----EEPFQ 56

Query: 316  INSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKNVWE 495
            ++ ++  LST+ FG+ LI+SP+L STHDVVS NF ELPIGTVCVADVQ KGRGRSKN WE
Sbjct: 57   MDLFMSSLSTNTFGRFLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSKNKWE 116

Query: 496  SPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDLYLD 675
            SP+GCLLFSFT+QMEDGR VPL+QYVVSLA+TEAI  +C   G+P LDV+IKWPNDLYL+
Sbjct: 117  SPRGCLLFSFTLQMEDGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPNDLYLN 176

Query: 676  GLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEIIAAF 855
            GLKVGGILCTSTYKS+KFNVSAGIGLNV+N+KPT CLNAVL+ L  VA Q +RE+I+AAF
Sbjct: 177  GLKVGGILCTSTYKSRKFNVSAGIGLNVDNEKPTTCLNAVLRELSSVAYQFRREDIVAAF 236

Query: 856  FNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLTTSG 1035
            FNKFE F+ +FI QGFQ LEELY KTWLHSGQRVIVQE  E   Q+ E+VVTI+GL +SG
Sbjct: 237  FNKFETFFSLFINQGFQTLEELYCKTWLHSGQRVIVQENIE--GQMVENVVTIQGLASSG 294

Query: 1036 YLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            YLLA  +DGQ CELHPDGNSFDFFKGL+RRK
Sbjct: 295  YLLAIGDDGQMCELHPDGNSFDFFKGLIRRK 325


>ref|XP_004250339.1| PREDICTED: biotin--protein ligase-like [Solanum lycopersicum]
          Length = 374

 Score =  464 bits (1194), Expect = e-128
 Identities = 235/363 (64%), Positives = 287/363 (79%), Gaps = 15/363 (4%)
 Frame = +1

Query: 85   SLQKFSFTPN-------KPY-----LSAKMEPQSS--TMLVVCGKSAAENELARCLKNNN 222
            SL+KF   P+       K Y     L  KME ++   T+L++ GKS  EN++A+ LK+NN
Sbjct: 13   SLRKFPHFPSIFPLPHLKAYSASQVLGGKMESEAPDRTLLLLAGKSNEENDVAKVLKDNN 72

Query: 223  TLKL-PDDSPVTVVLQSEIESQENVGNDDIFRINSYVDLLSTSRFGKLLIYSPKLASTHD 399
            TL+L  +   V V+L SE+E+      DD F+I  Y + LST RFG+LLIYSPKL+STHD
Sbjct: 73   TLQLRKNGEEVEVILPSEVET---TFEDDAFQIGEYFNSLSTKRFGRLLIYSPKLSSTHD 129

Query: 400  VVSSNFMELPIGTVCVADVQFKGRGRSKNVWESPKGCLLFSFTIQMEDGRIVPLVQYVVS 579
            V+S N  +LP+GTVCVADVQ KGRGRS N+W+SPKGCLLFSF+IQMEDGR+VP +QYVV 
Sbjct: 130  VISQNNSDLPVGTVCVADVQLKGRGRSSNLWQSPKGCLLFSFSIQMEDGRVVPHLQYVVC 189

Query: 580  LAMTEAINDVCRRNGMPSLDVRIKWPNDLYLDGLKVGGILCTSTYKSKKFNVSAGIGLNV 759
            LAMTEAI  +C   GMP LDVRIKWPNDLYL GLKVGGIL TS Y+SKKF VSAG+GLNV
Sbjct: 190  LAMTEAIKAICLEKGMPHLDVRIKWPNDLYLGGLKVGGILSTSVYRSKKFYVSAGVGLNV 249

Query: 760  NNQKPTICLNAVLQRLGCVAPQVQREEIIAAFFNKFECFYDIFITQGFQALEELYYKTWL 939
             N+KPT CLNA L+R+  ++ +++RE+IIAAFFNKFE  YD+F+ QGFQALEELYY+TWL
Sbjct: 250  GNEKPTTCLNATLKRVNPLSRELKREDIIAAFFNKFENLYDVFLKQGFQALEELYYQTWL 309

Query: 940  HSGQRVIVQEKNEDQDQISEHVVTIKGLTTSGYLLAATEDGQTCELHPDGNSFDFFKGLV 1119
            HSGQRVIVQE+++DQDQ  E+VVTI+GLT+SGYLL  T+DGQ CELHPDGNSFDFFKGL+
Sbjct: 310  HSGQRVIVQERSDDQDQFVENVVTIQGLTSSGYLLGITDDGQMCELHPDGNSFDFFKGLI 369

Query: 1120 RRK 1128
            +RK
Sbjct: 370  KRK 372


>ref|XP_006351872.1| PREDICTED: biotin--protein ligase-like [Solanum tuberosum]
          Length = 374

 Score =  462 bits (1190), Expect = e-128
 Identities = 233/355 (65%), Positives = 284/355 (80%), Gaps = 3/355 (0%)
 Frame = +1

Query: 73   LPFLSLQKFSFTPNKPYLSAKMEPQSS--TMLVVCGKSAAENELARCLKNNNTLKL-PDD 243
            LP   L+ FS +     L  KME ++   T+L++ GKS  EN++A+ LK+NNTL+L  + 
Sbjct: 24   LPLPHLKAFSASH---VLGGKMESEAPDRTLLLLAGKSNEENDVAKVLKDNNTLQLRKNG 80

Query: 244  SPVTVVLQSEIESQENVGNDDIFRINSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFME 423
              V VVL+ E+E+      DD FRI  Y + LST RFG+LLIYSPKL+STHDV+S N  +
Sbjct: 81   EEVEVVLRPELETPFE---DDAFRIGEYFNSLSTKRFGRLLIYSPKLSSTHDVISQNNSD 137

Query: 424  LPIGTVCVADVQFKGRGRSKNVWESPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAIN 603
            LP+GTVCVADVQ KGRGRS N+W+SPKGCLLFSF+IQMEDGR+VP +QYVV LAMTEAI 
Sbjct: 138  LPVGTVCVADVQLKGRGRSSNLWQSPKGCLLFSFSIQMEDGRVVPHLQYVVCLAMTEAIK 197

Query: 604  DVCRRNGMPSLDVRIKWPNDLYLDGLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTIC 783
             +C   G P LDVRIKWPNDLYL GLKVGGIL TS Y+SKKF VSAG+GLNV N+KPT C
Sbjct: 198  AICLEKGTPHLDVRIKWPNDLYLGGLKVGGILSTSVYRSKKFYVSAGVGLNVGNEKPTTC 257

Query: 784  LNAVLQRLGCVAPQVQREEIIAAFFNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIV 963
            LNA L+RL  ++ +++RE+IIAAFFNKFE  YD+F+ QGFQALEELYY+TWLHSGQRVIV
Sbjct: 258  LNAALKRLNPLSRELKREDIIAAFFNKFENLYDVFLKQGFQALEELYYQTWLHSGQRVIV 317

Query: 964  QEKNEDQDQISEHVVTIKGLTTSGYLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            Q++++DQDQ  E+VVTI+GLT+SGYLL  T+DGQ CELHPDGNSFDFFKGL++RK
Sbjct: 318  QDRSDDQDQFVENVVTIQGLTSSGYLLGITDDGQMCELHPDGNSFDFFKGLIKRK 372


>ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis lyrata subsp. lyrata]
            gi|297339768|gb|EFH70185.1| holocarboxylase synthetase 2
            [Arabidopsis lyrata subsp. lyrata]
          Length = 328

 Score =  450 bits (1157), Expect = e-124
 Identities = 215/331 (64%), Positives = 278/331 (83%)
 Frame = +1

Query: 136  MEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQSEIESQENVGNDDIFR 315
            M+  +S  LV+CGKS+ E + A  LKNNN LKLPD++ V++ LQSEI++   V +DD F 
Sbjct: 1    MDFDASCSLVLCGKSSVETDAATRLKNNNVLKLPDNTKVSIFLQSEIKNL--VKDDDSFN 58

Query: 316  INSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCVADVQFKGRGRSKNVWE 495
            ++ +++ +ST RFG+ LI+SP L+STHDVVS NF E+P+G+VCV D+QFKGRGR+KNVWE
Sbjct: 59   LSLFMNSISTHRFGRFLIWSPFLSSTHDVVSHNFSEIPVGSVCVTDIQFKGRGRTKNVWE 118

Query: 496  SPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGMPSLDVRIKWPNDLYLD 675
            SPKGCL++SFT++MEDGR+VPL+QYVVSLA+TEA+ DVC + G+  +DV+IKWPNDLYL+
Sbjct: 119  SPKGCLMYSFTVEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYIDVKIKWPNDLYLN 178

Query: 676  GLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRLGCVAPQVQREEIIAAF 855
            GLKVGGILCTSTY S+KF+VS G+GLNV+N+KPT CLNAVL+     +  ++REEI+ AF
Sbjct: 179  GLKVGGILCTSTYGSRKFHVSVGVGLNVDNEKPTTCLNAVLKENCPASNLLKREEILGAF 238

Query: 856  FNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQDQISEHVVTIKGLTTSG 1035
            F+KFE F+D+F+ QGF++LEELYY+TWLHSGQRVIV+EKNE  DQ+ ++VVTI+GLT+SG
Sbjct: 239  FHKFETFFDLFMEQGFKSLEELYYRTWLHSGQRVIVEEKNE--DQVVQNVVTIQGLTSSG 296

Query: 1036 YLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            YLLA  +D    ELHPDGNSFDFFKGLVRRK
Sbjct: 297  YLLAIGDDNVMYELHPDGNSFDFFKGLVRRK 327


>gb|AAL93108.1|AF414937_1 holocarboxylase synthetase 1 [Arabidopsis thaliana]
          Length = 367

 Score =  449 bits (1155), Expect = e-124
 Identities = 214/347 (61%), Positives = 289/347 (83%)
 Frame = +1

Query: 88   LQKFSFTPNKPYLSAKMEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQ 267
            L++ SF+    + ++ ME  +S  LV+CGKS+ E E+A+ LKN N+LKLPD++ V+++L+
Sbjct: 27   LRRLSFS----FSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILE 82

Query: 268  SEIESQENVGNDDIFRINSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCV 447
            SE ++     +D+ F ++ +++ + T RFG+ LI+SP+L+STHDVVS NF ELP+G+VCV
Sbjct: 83   SEAKNLVK-DDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCV 141

Query: 448  ADVQFKGRGRSKNVWESPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGM 627
             D+QFKGRGR+KNVWESPKGCL++SFT++MEDGR+VPL+QYVVSLA+TEA+ DVC + G+
Sbjct: 142  TDIQFKGRGRTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGL 201

Query: 628  PSLDVRIKWPNDLYLDGLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRL 807
            P +DV+IKWPNDLY++GLKVGGILCTSTY+SKKFNVS G+GLNV+N +PT CLNAVL+ +
Sbjct: 202  PYIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGM 261

Query: 808  GCVAPQVQREEIIAAFFNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQD 987
               +  ++REEI+ AFF+KFE F+D+F+ QGF++LEELYY+TWLHS QRVIV++K E  D
Sbjct: 262  APESNLLKREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVE--D 319

Query: 988  QISEHVVTIKGLTTSGYLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            Q+ ++VVTI+GLT+SGYLLA  +D Q  ELHPDGNSFDFFKGLVRRK
Sbjct: 320  QVVQNVVTIQGLTSSGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRK 366


>ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thaliana]
            gi|30682866|ref|NP_850067.1| holocarboxylase synthase 1
            [Arabidopsis thaliana] gi|1688072|gb|AAC49706.1| biotin
            holocarboxylase synthetase [Arabidopsis thaliana]
            gi|20197509|gb|AAD31371.2| biotin holocarboxylase
            synthetase [Arabidopsis thaliana]
            gi|89000993|gb|ABD59086.1| At2g25710 [Arabidopsis
            thaliana] gi|330252646|gb|AEC07740.1| holocarboxylase
            synthase 1 [Arabidopsis thaliana]
            gi|330252647|gb|AEC07741.1| holocarboxylase synthase 1
            [Arabidopsis thaliana]
          Length = 367

 Score =  449 bits (1154), Expect = e-123
 Identities = 214/347 (61%), Positives = 288/347 (82%)
 Frame = +1

Query: 88   LQKFSFTPNKPYLSAKMEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQ 267
            L + SF+    + ++ ME  +S  LV+CGKS+ E E+A+ LKN N+LKLPD++ V+++L+
Sbjct: 27   LHRLSFS----FSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILE 82

Query: 268  SEIESQENVGNDDIFRINSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCV 447
            SE ++     +D+ F ++ +++ + T RFG+ LI+SP+L+STHDVVS NF ELP+G+VCV
Sbjct: 83   SEAKNLVK-DDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCV 141

Query: 448  ADVQFKGRGRSKNVWESPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGM 627
             D+QFKGRGR+KNVWESPKGCL++SFT++MEDGR+VPL+QYVVSLA+TEA+ DVC + G+
Sbjct: 142  TDIQFKGRGRTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGL 201

Query: 628  PSLDVRIKWPNDLYLDGLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRL 807
            P +DV+IKWPNDLY++GLKVGGILCTSTY+SKKFNVS G+GLNV+N +PT CLNAVL+ +
Sbjct: 202  PYIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGM 261

Query: 808  GCVAPQVQREEIIAAFFNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQD 987
               +  ++REEI+ AFF+KFE F+D+F+ QGF++LEELYY+TWLHS QRVIV++K E  D
Sbjct: 262  APESNLLKREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVE--D 319

Query: 988  QISEHVVTIKGLTTSGYLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            Q+ ++VVTI+GLT+SGYLLA  +D Q  ELHPDGNSFDFFKGLVRRK
Sbjct: 320  QVVQNVVTIQGLTSSGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRK 366


>ref|XP_002878877.1| holocarboxylase synthetase 1 [Arabidopsis lyrata subsp. lyrata]
            gi|297324716|gb|EFH55136.1| holocarboxylase synthetase 1
            [Arabidopsis lyrata subsp. lyrata]
          Length = 365

 Score =  444 bits (1142), Expect = e-122
 Identities = 213/347 (61%), Positives = 286/347 (82%)
 Frame = +1

Query: 88   LQKFSFTPNKPYLSAKMEPQSSTMLVVCGKSAAENELARCLKNNNTLKLPDDSPVTVVLQ 267
            L++ SF+ +    ++ ME  +S  LV+ GKS+ E E+A+CLKN N+L LPD++ V++ L+
Sbjct: 26   LRRLSFSVS----ASAMESDASCNLVLYGKSSVETEIAKCLKNKNSLTLPDNTKVSLFLE 81

Query: 268  SEIESQENVGNDDIFRINSYVDLLSTSRFGKLLIYSPKLASTHDVVSSNFMELPIGTVCV 447
            SE ++   V +D  F ++ +++ + T RFG+ LI+SP+L+STHDVVS NF ELP+G+VCV
Sbjct: 82   SEAKNL--VKDDGSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSQNFSELPVGSVCV 139

Query: 448  ADVQFKGRGRSKNVWESPKGCLLFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCRRNGM 627
             D+QFKGRGR+KNVWESPKGCL++SFT++MEDGR+VPL+QYVVSLA+TEA+ DVC + G+
Sbjct: 140  TDIQFKGRGRTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGL 199

Query: 628  PSLDVRIKWPNDLYLDGLKVGGILCTSTYKSKKFNVSAGIGLNVNNQKPTICLNAVLQRL 807
            P +DV+IKWPNDLYL+GLKVGGILCTSTY+SKKF+VS G+GLNV+N++PT CLNAVL+ +
Sbjct: 200  PYIDVKIKWPNDLYLNGLKVGGILCTSTYRSKKFHVSVGVGLNVDNEQPTTCLNAVLKGM 259

Query: 808  GCVAPQVQREEIIAAFFNKFECFYDIFITQGFQALEELYYKTWLHSGQRVIVQEKNEDQD 987
               +  ++REEI+ AFF+KFE F+D+F+ QGF++LEELYY+TWLHS QRV V+EK E  D
Sbjct: 260  SPESTLLKREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVTVEEKVE--D 317

Query: 988  QISEHVVTIKGLTTSGYLLAATEDGQTCELHPDGNSFDFFKGLVRRK 1128
            Q+ ++VVTI+GL +SGYLLA  +D Q  ELHPDGNSFDFFKGLVRRK
Sbjct: 318  QVVQNVVTIQGLASSGYLLAIGDDNQMYELHPDGNSFDFFKGLVRRK 364