BLASTX nr result
ID: Rehmannia23_contig00030197
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia23_contig00030197
(464 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003594727.1| Pectinesterase [Medicago truncatula] gi|3554... 163 2e-38
ref|XP_004486539.1| PREDICTED: probable pectinesterase 67-like [... 161 7e-38
ref|XP_002272939.1| PREDICTED: probable pectinesterase 67 [Vitis... 160 1e-37
ref|XP_002311381.2| hypothetical protein POPTR_0008s10440g [Popu... 159 3e-37
ref|XP_006488988.1| PREDICTED: probable pectinesterase 67-like [... 156 2e-36
ref|XP_006419374.1| hypothetical protein CICLE_v10006909mg [Citr... 156 2e-36
ref|XP_003533150.1| PREDICTED: probable pectinesterase 67-like [... 155 4e-36
ref|XP_004252928.1| PREDICTED: probable pectinesterase 67-like [... 154 1e-35
gb|ACU23222.1| unknown [Glycine max] 154 1e-35
ref|XP_002523016.1| Pectinesterase-2 precursor, putative [Ricinu... 154 2e-35
ref|XP_006406773.1| hypothetical protein EUTSA_v10021065mg [Eutr... 151 1e-34
ref|XP_006299552.1| hypothetical protein CARUB_v10015726mg [Caps... 150 2e-34
ref|XP_002885186.1| pectinesterase family protein [Arabidopsis l... 150 2e-34
gb|EMJ28056.1| hypothetical protein PRUPE_ppa017141mg [Prunus pe... 149 4e-34
gb|EOY06742.1| Pectin lyase-like superfamily protein isoform 2 [... 149 5e-34
gb|EOY06741.1| Pectin lyase-like superfamily protein isoform 1 [... 149 5e-34
ref|NP_188331.1| putative pectinesterase [Arabidopsis thaliana] ... 148 6e-34
ref|XP_004173405.1| PREDICTED: probable pectinesterase 67-like [... 146 3e-33
ref|XP_004295071.1| PREDICTED: probable pectinesterase 67-like [... 143 3e-32
gb|ABM68552.1| pectin esterase family protein [Lilium longiflorum] 143 3e-32
>ref|XP_003594727.1| Pectinesterase [Medicago truncatula] gi|355483775|gb|AES64978.1|
Pectinesterase [Medicago truncatula]
Length = 350
Score = 163 bits (413), Expect = 2e-38
Identities = 66/101 (65%), Positives = 85/101 (84%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
+F+ GK YG+G V+LGRAKG +SRVIFA TYLS+T++P+GWT WSY G T++L+HAEYKC
Sbjct: 250 IFIKGKVYGIGGVYLGRAKGPYSRVIFAKTYLSKTIVPEGWTNWSYDGSTEHLYHAEYKC 309
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPG+ ++ RA WS+QL +EEA PF+SID+I GK WLP WL
Sbjct: 310 HGPGAIAEKRASWSRQLSDEEAAPFISIDYIDGKNWLPAWL 350
>ref|XP_004486539.1| PREDICTED: probable pectinesterase 67-like [Cicer arietinum]
Length = 349
Score = 161 bits (408), Expect = 7e-38
Identities = 64/100 (64%), Positives = 83/100 (83%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
+F+ G+ YG+G V+LGRAKG +SRVIFA TYLS+T++P+GWT WSY G T+NL+HAEYKC
Sbjct: 249 IFIKGRVYGIGGVYLGRAKGPYSRVIFAKTYLSKTIVPEGWTNWSYDGSTENLYHAEYKC 308
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTW 301
HGPG+ S+ RA WS+QL +E+ PFLS+D+I GK WLP W
Sbjct: 309 HGPGATSEGRAPWSRQLSDEDVAPFLSVDYIDGKNWLPAW 348
>ref|XP_002272939.1| PREDICTED: probable pectinesterase 67 [Vitis vinifera]
gi|296083238|emb|CBI22874.3| unnamed protein product
[Vitis vinifera]
Length = 348
Score = 160 bits (406), Expect = 1e-37
Identities = 66/101 (65%), Positives = 84/101 (83%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VFV GK YG+G V+LGRAKG+HSR +FA Y+SRT++PQGWTKWSY G T+NL AEYKC
Sbjct: 248 VFVKGKVYGIGGVYLGRAKGSHSRAVFAKVYMSRTIVPQGWTKWSYTGSTENLFQAEYKC 307
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
+GPG+ ++NRA WS QL +EEA P+LS+DF+ G++WLP WL
Sbjct: 308 YGPGAETENRASWSLQLTDEEAAPYLSVDFVDGQKWLPAWL 348
>ref|XP_002311381.2| hypothetical protein POPTR_0008s10440g [Populus trichocarpa]
gi|550332785|gb|EEE88748.2| hypothetical protein
POPTR_0008s10440g [Populus trichocarpa]
Length = 352
Score = 159 bits (403), Expect = 3e-37
Identities = 68/100 (68%), Positives = 82/100 (82%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VF+ GK YG+GNV+LGRAKG +SRV+FA YLS+T+ P+GWT WSY G+T+NL+ AEYKC
Sbjct: 251 VFIKGKFYGIGNVYLGRAKGAYSRVVFAKAYLSKTIAPKGWTNWSYAGKTENLYQAEYKC 310
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTW 301
HGPG+ +NRA WSKQL EEA F+SIDFI GKEWLP W
Sbjct: 311 HGPGADPENRAPWSKQLTEEEAKSFMSIDFIDGKEWLPVW 350
>ref|XP_006488988.1| PREDICTED: probable pectinesterase 67-like [Citrus sinensis]
Length = 351
Score = 156 bits (395), Expect = 2e-36
Identities = 65/101 (64%), Positives = 81/101 (80%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VF+ GK YG G+V+LGRAKG +SRV+FA TYLSRT++P GWT WSY G TDNL AEY+C
Sbjct: 251 VFIEGKVYGTGDVYLGRAKGAYSRVVFAKTYLSRTIVPHGWTNWSYVGSTDNLFQAEYRC 310
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPG+ +K+R WSKQL ++EA F+SIDF+ GK WLP W+
Sbjct: 311 HGPGAEAKHRVPWSKQLSDQEAEKFMSIDFVDGKNWLPAWV 351
>ref|XP_006419374.1| hypothetical protein CICLE_v10006909mg [Citrus clementina]
gi|557521247|gb|ESR32614.1| hypothetical protein
CICLE_v10006909mg [Citrus clementina]
Length = 351
Score = 156 bits (395), Expect = 2e-36
Identities = 65/101 (64%), Positives = 81/101 (80%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VF+ GK YG G+V+LGRAKG +SRV+FA TYLSRT++P GWT WSY G TDNL AEY+C
Sbjct: 251 VFIEGKVYGTGDVYLGRAKGAYSRVVFAKTYLSRTIVPHGWTNWSYVGSTDNLFQAEYRC 310
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPG+ +K+R WSKQL ++EA F+SIDF+ GK WLP W+
Sbjct: 311 HGPGAEAKHRVPWSKQLSDQEAEKFMSIDFVDGKNWLPAWV 351
>ref|XP_003533150.1| PREDICTED: probable pectinesterase 67-like [Glycine max]
Length = 346
Score = 155 bits (393), Expect = 4e-36
Identities = 63/101 (62%), Positives = 81/101 (80%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
+F+ GK YG+G V+LGRAKG +SRVIFA TYLS+T++P+GWT WSY G T +L+HAEY+C
Sbjct: 246 IFIKGKVYGIGGVYLGRAKGPYSRVIFAETYLSKTIVPEGWTNWSYDGSTKDLYHAEYEC 305
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPG+ + RA WS+QL EE PF+SID+I GK WLP W+
Sbjct: 306 HGPGALTTGRAPWSRQLTKEEVAPFISIDYIDGKNWLPAWV 346
>ref|XP_004252928.1| PREDICTED: probable pectinesterase 67-like [Solanum lycopersicum]
Length = 342
Score = 154 bits (389), Expect = 1e-35
Identities = 66/101 (65%), Positives = 81/101 (80%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VFV GK YG+G V+LGRA G +SRVIFA TY S+T++PQGWT WS G T NL+H EYKC
Sbjct: 242 VFVEGKVYGIGGVYLGRAIGAYSRVIFAKTYFSKTIVPQGWTNWSSPGSTKNLYHGEYKC 301
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPGSA + RA WSKQL ++EA+ F SI+FI+GK+WLP W+
Sbjct: 302 HGPGSAIETRATWSKQLNDKEAMAFTSIEFIQGKQWLPAWI 342
>gb|ACU23222.1| unknown [Glycine max]
Length = 346
Score = 154 bits (389), Expect = 1e-35
Identities = 62/101 (61%), Positives = 80/101 (79%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
+F+ GK YG+G V+LGRAKG +SRVIF TYLS+T++P+GWT WSY G T +L+HAEY+C
Sbjct: 246 IFIKGKVYGIGGVYLGRAKGPYSRVIFVETYLSKTIVPEGWTNWSYDGSTKDLYHAEYEC 305
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPG+ + RA WS+QL EE PF+SID+I GK WLP W+
Sbjct: 306 HGPGALTTGRAPWSRQLTKEEVAPFISIDYIDGKNWLPAWV 346
>ref|XP_002523016.1| Pectinesterase-2 precursor, putative [Ricinus communis]
gi|223537738|gb|EEF39358.1| Pectinesterase-2 precursor,
putative [Ricinus communis]
Length = 350
Score = 154 bits (388), Expect = 2e-35
Identities = 66/101 (65%), Positives = 80/101 (79%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VFV GK YG+G+V+LGRAKG +SR IFA TYLSRT+ P+GWT WSY G T+NL AEYKC
Sbjct: 250 VFVKGKVYGIGDVYLGRAKGAYSRTIFAKTYLSRTIDPRGWTNWSYSGTTENLFQAEYKC 309
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPG+ + +R W+KQL EA PF+SIDFI G++WLP WL
Sbjct: 310 HGPGADTTDRVEWAKQLTEAEAEPFMSIDFIDGQQWLPVWL 350
>ref|XP_006406773.1| hypothetical protein EUTSA_v10021065mg [Eutrema salsugineum]
gi|557107919|gb|ESQ48226.1| hypothetical protein
EUTSA_v10021065mg [Eutrema salsugineum]
Length = 344
Score = 151 bits (381), Expect = 1e-34
Identities = 64/101 (63%), Positives = 78/101 (77%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VF+ GK YG+ V+LGRAKG +SRVIFA TYLS+TV+P GWT WSY G T NL+HAEYKC
Sbjct: 240 VFIRGKVYGIDEVYLGRAKGPYSRVIFAKTYLSKTVVPNGWTNWSYDGSTQNLYHAEYKC 299
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPG+ ++R+ W+K+L +E FLSIDFI G WLP WL
Sbjct: 300 HGPGAERQHRSDWAKELTKQEVESFLSIDFIDGTSWLPVWL 340
>ref|XP_006299552.1| hypothetical protein CARUB_v10015726mg [Capsella rubella]
gi|482568261|gb|EOA32450.1| hypothetical protein
CARUB_v10015726mg [Capsella rubella]
Length = 344
Score = 150 bits (378), Expect = 2e-34
Identities = 63/101 (62%), Positives = 77/101 (76%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VF+ GK YG+ V+LGRAKG +SRVIFA TYLS+TV+P GWT WSY G T+NL+H EYKC
Sbjct: 240 VFIRGKVYGIDEVYLGRAKGPYSRVIFAKTYLSKTVVPDGWTNWSYHGSTENLYHGEYKC 299
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPG+ + R+ W+K+L +E FLSIDFI G WLP WL
Sbjct: 300 HGPGAERQQRSDWAKELTKQEVESFLSIDFIDGTSWLPVWL 340
>ref|XP_002885186.1| pectinesterase family protein [Arabidopsis lyrata subsp. lyrata]
gi|297331026|gb|EFH61445.1| pectinesterase family
protein [Arabidopsis lyrata subsp. lyrata]
Length = 344
Score = 150 bits (378), Expect = 2e-34
Identities = 63/101 (62%), Positives = 77/101 (76%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VF+ GK YG+ V+LGRAKG +SRVIFA TYLS+TV+P GWT WSY G T+NL+H EYKC
Sbjct: 240 VFIRGKVYGIDEVYLGRAKGPYSRVIFAKTYLSKTVVPDGWTNWSYHGSTENLYHGEYKC 299
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPG+ + R+ W+K+L +E FLSIDFI G WLP WL
Sbjct: 300 HGPGAERQKRSDWAKELTKQEVESFLSIDFIDGTSWLPVWL 340
>gb|EMJ28056.1| hypothetical protein PRUPE_ppa017141mg [Prunus persica]
Length = 345
Score = 149 bits (376), Expect = 4e-34
Identities = 67/102 (65%), Positives = 81/102 (79%), Gaps = 1/102 (0%)
Frame = +2
Query: 2 VFVNGKAYGVGN-VHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYK 178
VF GK YGVG+ V+LGRAKG SRV++A+ YLS+TV+PQGWT WSY G T+ L+HAEYK
Sbjct: 244 VFNKGKLYGVGDTVYLGRAKGAFSRVVYAHMYLSKTVVPQGWTNWSYAGGTETLYHAEYK 303
Query: 179 CHGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
C GPG+ + RA W+KQL +EA PFLSIDFI G+EWLP WL
Sbjct: 304 CKGPGAEAGGRAEWAKQLTEKEAAPFLSIDFINGQEWLPVWL 345
>gb|EOY06742.1| Pectin lyase-like superfamily protein isoform 2 [Theobroma cacao]
Length = 337
Score = 149 bits (375), Expect = 5e-34
Identities = 63/101 (62%), Positives = 77/101 (76%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VFV GK YG+G V+LGRAKG +S V+FA TYLSRT++P GWT WSY G +D+L HAEY C
Sbjct: 237 VFVEGKVYGIGGVYLGRAKGAYSTVVFAKTYLSRTIVPHGWTDWSYHGGSDHLFHAEYDC 296
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
GPG+ + R HW+KQL +EA + SIDFI GK+WLP WL
Sbjct: 297 RGPGADTSERGHWAKQLTEKEAAYWTSIDFINGKDWLPVWL 337
>gb|EOY06741.1| Pectin lyase-like superfamily protein isoform 1 [Theobroma cacao]
Length = 410
Score = 149 bits (375), Expect = 5e-34
Identities = 63/101 (62%), Positives = 77/101 (76%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VFV GK YG+G V+LGRAKG +S V+FA TYLSRT++P GWT WSY G +D+L HAEY C
Sbjct: 310 VFVEGKVYGIGGVYLGRAKGAYSTVVFAKTYLSRTIVPHGWTDWSYHGGSDHLFHAEYDC 369
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
GPG+ + R HW+KQL +EA + SIDFI GK+WLP WL
Sbjct: 370 RGPGADTSERGHWAKQLTEKEAAYWTSIDFINGKDWLPVWL 410
>ref|NP_188331.1| putative pectinesterase [Arabidopsis thaliana]
gi|75311531|sp|Q9LSP1.1|PME67_ARATH RecName:
Full=Probable pectinesterase 67; Short=PE 67; AltName:
Full=Pectin methylesterase 67; Short=AtPME67; Flags:
Precursor gi|7670030|dbj|BAA94984.1| pectinesterase-like
protein [Arabidopsis thaliana]
gi|21553361|gb|AAM62454.1| pectinesterase, putative
[Arabidopsis thaliana] gi|26451762|dbj|BAC42976.1|
putative pectinesterase [Arabidopsis thaliana]
gi|28973561|gb|AAO64105.1| putative pectinesterase
[Arabidopsis thaliana] gi|332642378|gb|AEE75899.1|
putative pectinesterase [Arabidopsis thaliana]
Length = 344
Score = 148 bits (374), Expect = 6e-34
Identities = 63/101 (62%), Positives = 75/101 (74%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VF+ GK YG+ V+LGRAKG +SRVIFA TYLS+TV+P GWT WSY G T NL+H EYKC
Sbjct: 240 VFIRGKVYGIDEVYLGRAKGPYSRVIFAKTYLSKTVVPDGWTNWSYHGSTQNLYHGEYKC 299
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
HGPG+ + R+ W+K L +E FLSIDFI G WLP WL
Sbjct: 300 HGPGAERQKRSDWAKDLTKQEVESFLSIDFIDGTSWLPVWL 340
>ref|XP_004173405.1| PREDICTED: probable pectinesterase 67-like [Cucumis sativus]
Length = 350
Score = 146 bits (368), Expect = 3e-33
Identities = 63/101 (62%), Positives = 75/101 (74%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VF+ GK YGVG +LGRAKG SRVIFA TY S +V+P GWT WS+ G T+NL+H EY C
Sbjct: 250 VFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTENLYHGEYDC 309
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLPTWL 304
+GPGS S NRA W+KQL EEA PF+ + FI G +WLP WL
Sbjct: 310 YGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
>ref|XP_004295071.1| PREDICTED: probable pectinesterase 67-like [Fragaria vesca subsp.
vesca]
Length = 348
Score = 143 bits (360), Expect = 3e-32
Identities = 62/99 (62%), Positives = 80/99 (80%), Gaps = 1/99 (1%)
Frame = +2
Query: 2 VFVNGKAYGVGN-VHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYK 178
VF+ GK YGVG+ V+LGRAKG SRV++A TYLSRT++P+GWT WSY G T+NL+HAEY
Sbjct: 247 VFIKGKVYGVGDHVYLGRAKGAFSRVVYAETYLSRTIMPEGWTNWSYAGGTENLYHAEYN 306
Query: 179 CHGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWLP 295
C GPG+ +R W+KQL ++E PFL+IDFI+GK+WLP
Sbjct: 307 CKGPGAEFSDRVPWAKQLTDQEVQPFLTIDFIEGKDWLP 345
>gb|ABM68552.1| pectin esterase family protein [Lilium longiflorum]
Length = 163
Score = 143 bits (360), Expect = 3e-32
Identities = 62/97 (63%), Positives = 73/97 (75%)
Frame = +2
Query: 2 VFVNGKAYGVGNVHLGRAKGTHSRVIFANTYLSRTVIPQGWTKWSYQGRTDNLHHAEYKC 181
VF+ G+ YGV +V LGRAKG HSRV+FA TYLSRT+ P GWT WSY G T+N+ H EY C
Sbjct: 67 VFLKGRVYGVSDVFLGRAKGAHSRVVFAKTYLSRTITPAGWTNWSYDGSTNNILHGEYDC 126
Query: 182 HGPGSASKNRAHWSKQLGNEEAVPFLSIDFIKGKEWL 292
GPG+ S R WS+QLG EA PFL++DFI GKEWL
Sbjct: 127 SGPGADSSLRVPWSRQLGENEAAPFLNVDFINGKEWL 163