BLASTX nr result
ID: Rehmannia23_contig00027408
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia23_contig00027408
(697 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 135 2e-29
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 135 2e-29
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 135 2e-29
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 133 5e-29
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 133 5e-29
ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 132 8e-29
emb|CBI34715.3| unnamed protein product [Vitis vinifera] 132 1e-28
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 132 1e-28
ref|XP_006358870.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 131 2e-28
ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 129 9e-28
ref|XP_004245646.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 126 6e-27
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 124 4e-26
ref|XP_002318833.1| hypothetical protein POPTR_0012s13580g [Popu... 124 4e-26
ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 123 6e-26
ref|XP_004234365.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 122 8e-26
ref|XP_006358915.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 121 2e-25
ref|XP_004234364.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 119 7e-25
ref|XP_006353349.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 119 1e-24
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 119 1e-24
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 118 2e-24
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 135 bits (339), Expect = 2e-29
Identities = 76/171 (44%), Positives = 103/171 (60%), Gaps = 1/171 (0%)
Frame = +3
Query: 183 KHFLFGSRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSP 362
KH LPPGP ++ L + PH L L++++GPLMS +LG QLMVVASSP
Sbjct: 569 KHIFLKPPPLPPGPYPWPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSP 628
Query: 363 DIAKEFCKTHDRIFSGRHL-PSVYHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSK 539
A E KTHDR SGR+L SV P + + SIV + DC+ +WK LR+I + +FS K
Sbjct: 629 AAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGK 688
Query: 540 AMESNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
AMES +R+ KV +LV L T EG + + D++F T+ NI+S S +L
Sbjct: 689 AMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDL 739
Score = 132 bits (333), Expect = 8e-29
Identities = 73/171 (42%), Positives = 104/171 (60%), Gaps = 1/171 (0%)
Frame = +3
Query: 183 KHFLFGSRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSP 362
KH LPPGP ++ L L PH L L++++GPLMS +LG QLMVVASSP
Sbjct: 1074 KHIFLKPPPLPPGPYPWPIIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSP 1133
Query: 363 DIAKEFCKTHDRIFSGRHL-PSVYHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSK 539
A E KTHDR SGR++ S+ P + + S+ + +C+ +WK LR+I + +FS K
Sbjct: 1134 AAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGK 1193
Query: 540 AMESNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
AMES+ +R+ KVM+LV L T EG + + D++F T+ NI+S S +L
Sbjct: 1194 AMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDL 1244
Score = 114 bits (286), Expect = 2e-23
Identities = 64/168 (38%), Positives = 98/168 (58%), Gaps = 1/168 (0%)
Frame = +3
Query: 183 KHFLFGSRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSP 362
KH S LPPGP ++ L + H L L++ +GPLMS +LG Q+MVVASS
Sbjct: 29 KHIFLKSPPLPPGPYPWPIIGNLLQMGGNLHVKLANLAKRHGPLMSLRLGTQIMVVASSS 88
Query: 363 DIAKEFCKTHDRIFSGRHL-PSVYHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSK 539
A E KTHDR SGR++ ++ N P + + ++ + C+ W+ L++I + +FS K
Sbjct: 89 AAAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGK 148
Query: 540 AMESNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTS 683
AMES +R+ KVM+LV L+ EG + + D+++ T+ NI+S S
Sbjct: 149 AMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFS 196
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 135 bits (339), Expect = 2e-29
Identities = 76/171 (44%), Positives = 103/171 (60%), Gaps = 1/171 (0%)
Frame = +3
Query: 183 KHFLFGSRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSP 362
KH LPPGP ++ L + PH L L++++GPLMS +LG QLMVVASSP
Sbjct: 29 KHIFLKPPPLPPGPYPWPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSP 88
Query: 363 DIAKEFCKTHDRIFSGRHL-PSVYHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSK 539
A E KTHDR SGR+L SV P + + SIV + DC+ +WK LR+I + +FS K
Sbjct: 89 AAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGK 148
Query: 540 AMESNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
AMES +R+ KV +LV L T EG + + D++F T+ NI+S S +L
Sbjct: 149 AMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDL 199
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 135 bits (339), Expect = 2e-29
Identities = 76/171 (44%), Positives = 103/171 (60%), Gaps = 1/171 (0%)
Frame = +3
Query: 183 KHFLFGSRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSP 362
KH LPPGP ++ L + PH L L++++GPLMS +LG QLMVVASSP
Sbjct: 29 KHIFLKPPPLPPGPYPWPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSP 88
Query: 363 DIAKEFCKTHDRIFSGRHL-PSVYHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSK 539
A E KTHDR SGR+L SV P + + SIV + DC+ +WK LR+I + +FS K
Sbjct: 89 AAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGK 148
Query: 540 AMESNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
AMES +R+ KV +LV L T EG + + D++F T+ NI+S S +L
Sbjct: 149 AMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDL 199
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 133 bits (335), Expect = 5e-29
Identities = 70/167 (41%), Positives = 107/167 (64%), Gaps = 4/167 (2%)
Frame = +3
Query: 201 SRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEF 380
S LPPGP+ +L L HL PH +L + SQ YGPL+S +LG Q++VVAS+ A E
Sbjct: 474 SPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEI 533
Query: 381 CKTHDRIFSGRHLPSVYHNLPG----IMNSSIVLSPDCSKSWKLLRSIGQNCVFSSKAME 548
KTHDR+ SGR++P H +P I S+ + +C+ +WK LR++ + +FS+K ME
Sbjct: 534 LKTHDRVLSGRYVP---HAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVME 590
Query: 549 SNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRN 689
S AW+ + KVM++V + T EG + + +++FAT+ N ++T L SR+
Sbjct: 591 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRD 637
>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 503
Score = 133 bits (335), Expect = 5e-29
Identities = 70/167 (41%), Positives = 107/167 (64%), Gaps = 4/167 (2%)
Frame = +3
Query: 201 SRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEF 380
S LPPGP+ +L L HL PH +L + SQ YGPL+S +LG Q++VVAS+ A E
Sbjct: 41 SPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEI 100
Query: 381 CKTHDRIFSGRHLPSVYHNLPG----IMNSSIVLSPDCSKSWKLLRSIGQNCVFSSKAME 548
KTHDR+ SGR++P H +P I S+ + +C+ +WK LR++ + +FS+K ME
Sbjct: 101 LKTHDRVLSGRYVP---HAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVME 157
Query: 549 SNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRN 689
S AW+ + KVM++V + T EG + + +++FAT+ N ++T L SR+
Sbjct: 158 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRD 204
>ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
[Vitis vinifera]
Length = 515
Score = 132 bits (333), Expect = 8e-29
Identities = 73/171 (42%), Positives = 104/171 (60%), Gaps = 1/171 (0%)
Frame = +3
Query: 183 KHFLFGSRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSP 362
KH LPPGP ++ L L PH L L++++GPLMS +LG QLMVVASSP
Sbjct: 29 KHIFLKPPPLPPGPYPWPIIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSP 88
Query: 363 DIAKEFCKTHDRIFSGRHL-PSVYHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSK 539
A E KTHDR SGR++ S+ P + + S+ + +C+ +WK LR+I + +FS K
Sbjct: 89 AAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGK 148
Query: 540 AMESNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
AMES+ +R+ KVM+LV L T EG + + D++F T+ NI+S S +L
Sbjct: 149 AMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDL 199
>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
Length = 1076
Score = 132 bits (332), Expect = 1e-28
Identities = 70/167 (41%), Positives = 107/167 (64%), Gaps = 4/167 (2%)
Frame = +3
Query: 201 SRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEF 380
S LPPGP+ +L L HL PH +L + SQ YGPL+S +LG Q++VVAS+ A E
Sbjct: 605 SPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEI 664
Query: 381 CKTHDRIFSGRHLPSVYHNLPG----IMNSSIVLSPDCSKSWKLLRSIGQNCVFSSKAME 548
KTHDR+ SGR++P H +P I S+ + +C+ +WK LR++ + +FS+K ME
Sbjct: 665 LKTHDRVLSGRYVP---HAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVME 721
Query: 549 SNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRN 689
S AW+ + KVM++V + T EG + + +++FAT+ N ++T L SR+
Sbjct: 722 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRD 768
>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Vitis vinifera]
Length = 503
Score = 132 bits (332), Expect = 1e-28
Identities = 70/167 (41%), Positives = 107/167 (64%), Gaps = 4/167 (2%)
Frame = +3
Query: 201 SRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEF 380
S LPPGP+ +L L HL PH +L + SQ YGPL+S +LG Q++VVAS+ A E
Sbjct: 41 SPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEI 100
Query: 381 CKTHDRIFSGRHLPSVYHNLPG----IMNSSIVLSPDCSKSWKLLRSIGQNCVFSSKAME 548
KTHDR+ SGR++P H +P I S+ + +C+ +WK LR++ + +FS+K ME
Sbjct: 101 LKTHDRVLSGRYVP---HAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVME 157
Query: 549 SNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRN 689
S AW+ + KVM++V + T EG + + +++FAT+ N ++T L SR+
Sbjct: 158 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRD 204
>ref|XP_006358870.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 495
Score = 131 bits (329), Expect = 2e-28
Identities = 66/163 (40%), Positives = 103/163 (63%), Gaps = 1/163 (0%)
Frame = +3
Query: 207 QLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEFCK 386
QLPPGP V+ + H+ PH L ++IYGPLMS +LG Q +VV SS A E K
Sbjct: 33 QLPPGPTPWPVIGNIFHMGKMPHITLTNFAKIYGPLMSLRLGTQCLVVGSSSSAAIEILK 92
Query: 387 THDRIFSGRHLPSVYHNLPGIMNS-SIVLSPDCSKSWKLLRSIGQNCVFSSKAMESNAWI 563
THDRI SGRH+P+ ++ S+ + +C W+ LR++ + +FS K +ES A++
Sbjct: 93 THDRILSGRHVPNAMPAKRSDLDKISMGWNSECHNEWRCLRTLCKTELFSGKVLESQAYL 152
Query: 564 RKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
R+ KVM+LV L++ EG + N+ +++FAT+ N++S L S+++
Sbjct: 153 REKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSNVLISKDM 195
>ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 496
Score = 129 bits (324), Expect = 9e-28
Identities = 63/163 (38%), Positives = 102/163 (62%), Gaps = 1/163 (0%)
Frame = +3
Query: 207 QLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEFCK 386
QLPPGP V+ + + PH L ++ YGPL+S +LG Q +VV SSP +A E K
Sbjct: 34 QLPPGPTPWPVIGNIFQIGKMPHITLTNFAKTYGPLISLRLGTQCLVVGSSPSVAIEIFK 93
Query: 387 THDRIFSGRHLPSVYHNL-PGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSKAMESNAWI 563
THDRI SGRH+P+ + + +S+ +C W+ LR++ + +F+ K +ES A +
Sbjct: 94 THDRILSGRHVPNAVRSKGSDLEKTSLGWKSECDSGWRYLRTLCRTDLFAGKVLESQACL 153
Query: 564 RKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
R+ KVM+LV L++ EG + N+ +++FAT+ N++S L S+++
Sbjct: 154 REKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSNVLISKDM 196
>ref|XP_004245646.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum lycopersicum]
Length = 496
Score = 126 bits (317), Expect = 6e-27
Identities = 68/164 (41%), Positives = 103/164 (62%), Gaps = 2/164 (1%)
Frame = +3
Query: 207 QLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEFCK 386
QLPPGP V+ + H+ PH L ++IYGPLMS +LG Q +VV SS A E K
Sbjct: 33 QLPPGPTPWPVIGNIFHMGKMPHITLTNFAKIYGPLMSLRLGSQCLVVGSSTSTAIEILK 92
Query: 387 THDRIFSGRHLP-SVYHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSKAMESNAWI 563
THDRI SGRH+P +V + S+ + +C W+ LR++ + +FS K +ES A++
Sbjct: 93 THDRILSGRHVPNAVPAKRSDLDKISMGWTSECHNEWRYLRTLCRAELFSGKVLESQAYL 152
Query: 564 RKAKVMDLVSHLQTNEGTIANL-EDMMFATLANIISTALTSRNL 692
R+ KVM+LV L++ EG + N+ E ++FAT+ N++S L S+++
Sbjct: 153 REKKVMELVEFLRSKEGQVVNIGELVVFATVLNMLSNVLISKDM 196
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 124 bits (310), Expect = 4e-26
Identities = 61/164 (37%), Positives = 106/164 (64%), Gaps = 1/164 (0%)
Frame = +3
Query: 204 RQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEFC 383
R LPPGP +L + + PH ++ ++++GPL+S +LG +++VVASSP A E
Sbjct: 35 RPLPPGPRQWPILGNILQVGKNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEIL 94
Query: 384 KTHDRIFSGRHLPSVYHNLPGIMNS-SIVLSPDCSKSWKLLRSIGQNCVFSSKAMESNAW 560
KTHDR+ SGR++P+ +++ ++V +P CS WK LR++ ++ +FS+KA+ES A
Sbjct: 95 KTHDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQAT 154
Query: 561 IRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
+R+ K+ +++ L + +G I N+ +++F T N IS L S++L
Sbjct: 155 LREKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDL 198
>ref|XP_002318833.1| hypothetical protein POPTR_0012s13580g [Populus trichocarpa]
gi|222859506|gb|EEE97053.1| hypothetical protein
POPTR_0012s13580g [Populus trichocarpa]
Length = 503
Score = 124 bits (310), Expect = 4e-26
Identities = 65/165 (39%), Positives = 105/165 (63%), Gaps = 1/165 (0%)
Frame = +3
Query: 201 SRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEF 380
S+ LPPGP ++ L H+ +PH +L ++++I+GPL+S +LG QL+VV SS A E
Sbjct: 41 SKYLPPGPKPWPIIGNLLHVGNQPHVSLAEIAKIHGPLISLRLGTQLLVVGSSAKAAAEI 100
Query: 381 CKTHDRIFSGRHLPSVYHNLPGIMNS-SIVLSPDCSKSWKLLRSIGQNCVFSSKAMESNA 557
KTHDR S RH+P V ++ ++V P+ +WKLLR + + +FS+KA+ES+A
Sbjct: 101 LKTHDRFLSARHVPQVIPRESHVLRRVALVWCPESIDTWKLLRGLCRTELFSAKAIESSA 160
Query: 558 WIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
+R+ KV +L+ L EG + ++ +++F+T+ N IS L S +L
Sbjct: 161 TLREKKVGELMDFLVAREGKVVSIGEVVFSTVFNTISNLLFSNDL 205
>ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 486
Score = 123 bits (308), Expect = 6e-26
Identities = 69/171 (40%), Positives = 102/171 (59%), Gaps = 1/171 (0%)
Frame = +3
Query: 183 KHFLFGSRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSP 362
K + S LPPGP +L + + PH +L + ++IYGPL+S +LG QL+VVASSP
Sbjct: 22 KQLIPKSPNLPPGPRPWPLLGNILQIGKSPHISLAQFAKIYGPLISVKLGTQLVVVASSP 81
Query: 363 DIAKEFCKTHDRIFSGRHLPSV-YHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSK 539
D A E KT DR+ S R +P V + L I SIV S D S WK LR+ + +FS K
Sbjct: 82 DSAAEILKTQDRLLSVRSVPKVATYELSVIDQHSIVFSSDLSNHWKFLRAFCRTHLFSPK 141
Query: 540 AMESNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
A+ES A +R+ KV +++ L++ +G + ++MFAT+ N + S++L
Sbjct: 142 AVESQAALREKKVSEMIHFLRSRKGETVKISEIMFATILNTLGNLFFSKDL 192
>ref|XP_004234365.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Solanum lycopersicum]
Length = 486
Score = 122 bits (307), Expect = 8e-26
Identities = 70/171 (40%), Positives = 101/171 (59%), Gaps = 1/171 (0%)
Frame = +3
Query: 183 KHFLFGSRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSP 362
K F+ S LPPGP S +L + + PH +L K +QI+GPL+S +LG QL+VVASSP
Sbjct: 22 KQFIPKSGNLPPGPRSWPLLGNILQIGKNPHISLAKFAQIHGPLISVKLGTQLVVVASSP 81
Query: 363 DIAKEFCKTHDRIFSGRHLPSVY-HNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSK 539
A E KT DR+ S R PSV + L I SIV S D S WKLLR+ + +FS K
Sbjct: 82 ASAAEILKTQDRLLSARSPPSVAPYELSVINQHSIVFSSDLSNHWKLLRAFCRTHLFSPK 141
Query: 540 AMESNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
A+ES +R+ KV +++ L++ +G + +++F T+ N S++L
Sbjct: 142 AVESQTALREKKVSEMIHFLKSKKGETVMISEILFGTILNTFGNLFFSKDL 192
>ref|XP_006358915.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 495
Score = 121 bits (303), Expect = 2e-25
Identities = 62/163 (38%), Positives = 102/163 (62%), Gaps = 1/163 (0%)
Frame = +3
Query: 207 QLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEFCK 386
QLPPGP V+ + + PH L ++ YGPLMS +LG + +VV SSP A E K
Sbjct: 33 QLPPGPTPWPVIGNIFQMGKMPHITLTNFAKTYGPLMSLKLGTKWLVVGSSPLAAVEILK 92
Query: 387 THDRIFSGRHLPSVYHNLPGIMN-SSIVLSPDCSKSWKLLRSIGQNCVFSSKAMESNAWI 563
THD+I SGR +PS + ++ +S+ +C+ W+ LR++ + +F+ K +ES A +
Sbjct: 93 THDKILSGRDVPSAAPSKGSDLDKTSLGWISECNSGWRYLRTLCRTELFAGKVLESQACL 152
Query: 564 RKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
R+ KVM+LV L++ EG + N+ +++FAT+ N++S L S+++
Sbjct: 153 REKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSNVLISKDM 195
>ref|XP_004234364.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Solanum lycopersicum]
Length = 486
Score = 119 bits (299), Expect = 7e-25
Identities = 66/165 (40%), Positives = 100/165 (60%), Gaps = 1/165 (0%)
Frame = +3
Query: 201 SRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEF 380
S LPPGP ++L L + PH +L K +QI+GPL+S +LG QL+VVASSP A E
Sbjct: 28 STNLPPGPRPWHLLGNLLQIGKTPHVSLAKFAQIHGPLISVKLGTQLVVVASSPASAAEI 87
Query: 381 CKTHDRIFSGRHLPSV-YHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSKAMESNA 557
K+ DR+ S R +P V + L I SIV S D S WK LR+ + +FS KA+ES A
Sbjct: 88 LKSQDRLLSVRSVPKVATYELSVIDQHSIVFSSDLSNQWKFLRAFCRTHLFSPKAVESQA 147
Query: 558 WIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
+R+ KV ++++ L++ +G + +++F T+ N + S++L
Sbjct: 148 ALREKKVSEMMNFLRSRKGETVKISEILFGTILNTLGNLFFSKDL 192
>ref|XP_006353349.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 487
Score = 119 bits (297), Expect = 1e-24
Identities = 68/171 (39%), Positives = 98/171 (57%), Gaps = 1/171 (0%)
Frame = +3
Query: 183 KHFLFGSRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSP 362
K + S LPPGP VL + L PH +L K ++I+GPL+S +LG QL+VVASSP
Sbjct: 22 KQLIPKSGNLPPGPRPWPVLGNILQLGKNPHISLAKFAEIHGPLISVKLGTQLVVVASSP 81
Query: 363 DIAKEFCKTHDRIFSGRHLPSVY-HNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSK 539
A E KT DR+ S R PSV + L I SIV S D WK LR+ + +FS K
Sbjct: 82 HSAAEILKTQDRLLSARSPPSVVPYELSVIDQHSIVFSSDLGNHWKFLRAFCRTHLFSPK 141
Query: 540 AMESNAWIRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNL 692
A+ES A +R KV +++ L++ +G + +++F T+ N + S++L
Sbjct: 142 AVESQAALRDKKVSEMIHFLRSKKGETVKISEILFGTILNTLGNLFFSKDL 192
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
isozyme 2; AltName: Full=Cytochrome P450 80B2
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 119 bits (297), Expect = 1e-24
Identities = 63/164 (38%), Positives = 98/164 (59%)
Frame = +3
Query: 204 RQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEFC 383
+ LPPGP ++ L L KPH KL+Q YG L S +LG Q +VVASSP A E
Sbjct: 27 KNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEIL 86
Query: 384 KTHDRIFSGRHLPSVYHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSKAMESNAWI 563
KTHD+I SGR++ + + NS ++ +C+ +WKLLR + + +F+ K +ES + I
Sbjct: 87 KTHDKILSGRYVFQSFRVKEHVENS--IVWSECNDNWKLLRKVCRTELFTPKMIESQSEI 144
Query: 564 RKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNLF 695
R+AK ++V L+ EG + + +++F TL NI + S+++F
Sbjct: 145 REAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVF 188
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 118 bits (296), Expect = 2e-24
Identities = 62/165 (37%), Positives = 100/165 (60%)
Frame = +3
Query: 201 SRQLPPGPNSLYVLTYLSHLIYKPHRALQKLSQIYGPLMSFQLGDQLMVVASSPDIAKEF 380
++ LPPGP ++ L L KPH KL++ YG L + +LG Q +VVASSP A E
Sbjct: 27 NKDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEKYGELFTLKLGSQTVVVASSPAAAAEI 86
Query: 381 CKTHDRIFSGRHLPSVYHNLPGIMNSSIVLSPDCSKSWKLLRSIGQNCVFSSKAMESNAW 560
KT D+I SGR++ + ++NS ++ +C+++WKLLR + + +FS K +ES A+
Sbjct: 87 LKTRDKILSGRYVFQSFRVYEHVLNS--IVWSECNENWKLLRKVCRTELFSPKMIESQAY 144
Query: 561 IRKAKVMDLVSHLQTNEGTIANLEDMMFATLANIISTALTSRNLF 695
IR+AK +D+V L+ E + +++F TL NI + S+++F
Sbjct: 145 IREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLIFSKDVF 189