BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rauwolfia21_contig00017208
         (1404 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004250032.1| PREDICTED: pullulanase 1, chloroplastic-like...   558   e-156
ref|XP_006361707.1| PREDICTED: pullulanase 1, chloroplastic-like...   557   e-156
gb|EMJ21470.1| hypothetical protein PRUPE_ppa000905mg [Prunus pe...   542   e-151
ref|XP_006378784.1| hypothetical protein POPTR_0010s23600g [Popu...   527   e-147
ref|XP_002315334.2| pullulanase family protein [Populus trichoca...   527   e-147
gb|EOY18848.1| Limit dextrinase [Theobroma cacao]                     525   e-146
emb|CBI31395.3| unnamed protein product [Vitis vinifera]              524   e-146
ref|XP_002271820.2| PREDICTED: pullulanase 1, chloroplastic-like...   523   e-146
ref|XP_002532780.1| pullulanase, putative [Ricinus communis] gi|...   521   e-145
ref|XP_006485313.1| PREDICTED: pullulanase 1, chloroplastic-like...   520   e-145
ref|XP_006436527.1| hypothetical protein CICLE_v10031035mg [Citr...   520   e-145
ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic-like...   515   e-143
ref|XP_006485314.1| PREDICTED: pullulanase 1, chloroplastic-like...   513   e-143
ref|XP_004496127.1| PREDICTED: pullulanase 1, chloroplastic-like...   512   e-142
ref|XP_004307333.1| PREDICTED: pullulanase 1, chloroplastic-like...   511   e-142
ref|XP_004157562.1| PREDICTED: pullulanase 1, chloroplastic-like...   495   e-137
ref|XP_004142505.1| PREDICTED: pullulanase 1, chloroplastic-like...   495   e-137
ref|XP_006398895.1| hypothetical protein EUTSA_v10012585mg [Eutr...   479   e-133
emb|CAA58803.1| pullulanase [Spinacia oleracea]                       479   e-133
gb|AAO00771.1| pullulanase-like protein (starch debranching enzy...   479   e-132

>ref|XP_004250032.1| PREDICTED: pullulanase 1, chloroplastic-like [Solanum lycopersicum]
          Length = 951

 Score =  558 bits (1439), Expect = e-156
 Identities = 273/393 (69%), Positives = 319/393 (81%)
 Frame = +2

Query: 224  LTRRQLGAAGIATARTPTILCCFSAMPVEGPXXXXXXXXXQDSLFYSRAFWVSESIIAWN 403
            LTR + G    AT+R+  + CC S MPV+           QDSLFYSRAFWV++SIIAWN
Sbjct: 35   LTRYRSGFKS-ATSRSLNLRCC-STMPVQDQSESPSCS--QDSLFYSRAFWVTKSIIAWN 90

Query: 404  VEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSGLPDYVVKKFPHIGDYNAFSV 583
            V AG G CYLYASR A L + D+ IQG+DVKIKLE    GLP  V++KFPHI DY AF V
Sbjct: 91   VGAGEGECYLYASRKAGLCVGDDGIQGHDVKIKLERTNYGLPQQVIEKFPHIRDYAAFEV 150

Query: 584  PPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDELFSYNGPLGAVFSSEAVFLYL 763
            P  LD + L+KCQLA+ +F+S G C SATGLQLPG+LD+LFSY GPLG +F++EAV LYL
Sbjct: 151  PATLDFQSLVKCQLAVGIFNSYGKCASATGLQLPGILDDLFSYTGPLGGIFATEAVSLYL 210

Query: 764  WAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPRDWEGCYYVYEVFVYHPSTLK 943
            WAPTAQ V+A +YK+PS  DP EIVQLKEL+GVWSAKGPR WEGCYYVYEV VYHPSTL+
Sbjct: 211  WAPTAQAVQALIYKSPSEADPVEIVQLKELDGVWSAKGPRHWEGCYYVYEVSVYHPSTLR 270

Query: 944  IERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLANEKPSIDSFSDISIYELHVR 1123
            IE+C++NDPYARGLSADG++TLLVNLDSD  KPEGWDNL +EKP++ SFSD+SIYELHVR
Sbjct: 271  IEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNLQDEKPNLLSFSDVSIYELHVR 330

Query: 1124 DFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGITHVHLLPTFQFAGVDDEKHKW 1303
            DFSAND +V    +GGYLAFT QDSAGV HLKRLS+AGITHVHLLPT+QFAGV+DEKHKW
Sbjct: 331  DFSANDPTVPHEFQGGYLAFTSQDSAGVQHLKRLSNAGITHVHLLPTYQFAGVEDEKHKW 390

Query: 1304 KNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            K  + + L S PPDS++QQALITAI+DEDGYNW
Sbjct: 391  KYTDIEKLNSFPPDSEEQQALITAIQDEDGYNW 423


>ref|XP_006361707.1| PREDICTED: pullulanase 1, chloroplastic-like [Solanum tuberosum]
          Length = 951

 Score =  557 bits (1435), Expect = e-156
 Identities = 274/393 (69%), Positives = 318/393 (80%)
 Frame = +2

Query: 224  LTRRQLGAAGIATARTPTILCCFSAMPVEGPXXXXXXXXXQDSLFYSRAFWVSESIIAWN 403
            LTR + G     T+R+  + CC S MPV+           QDSLFYSRAFWV++SIIAWN
Sbjct: 35   LTRYRSGFKS-GTSRSLNLRCC-STMPVQDQSENPSCS--QDSLFYSRAFWVTKSIIAWN 90

Query: 404  VEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSGLPDYVVKKFPHIGDYNAFSV 583
            V AG G CYLYASR A+L +A + IQG+DVKIKLE    GLP  V++KFPHI DY AF V
Sbjct: 91   VGAGEGECYLYASRKAELCVAGDGIQGHDVKIKLERTNYGLPQQVIEKFPHIRDYAAFEV 150

Query: 584  PPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDELFSYNGPLGAVFSSEAVFLYL 763
            P  LD + L+KCQLA+ +F+S G C SATGLQLPG+LDELFSY GPLGAVF++EAV LYL
Sbjct: 151  PATLDFQSLVKCQLAVGIFNSYGKCASATGLQLPGILDELFSYTGPLGAVFATEAVSLYL 210

Query: 764  WAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPRDWEGCYYVYEVFVYHPSTLK 943
            WAPTAQ V A +YK+PS  DP EIVQLKEL+GVWSAKGP  WEGCYYVYEV VYHPSTL+
Sbjct: 211  WAPTAQAVEALIYKSPSEADPVEIVQLKELDGVWSAKGPMHWEGCYYVYEVSVYHPSTLR 270

Query: 944  IERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLANEKPSIDSFSDISIYELHVR 1123
            IE+C++NDPYARGLSADG++TLLVNLDSD  KPEGWDNL +EKP++ SFSD+SIYELHVR
Sbjct: 271  IEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNLQDEKPNLLSFSDVSIYELHVR 330

Query: 1124 DFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGITHVHLLPTFQFAGVDDEKHKW 1303
            DFSA+D +V    +GGYLAFT QDSAGV HLKRLSSAGITHVHLLPT+QFAGV+DEKHKW
Sbjct: 331  DFSASDPTVSHEFQGGYLAFTSQDSAGVQHLKRLSSAGITHVHLLPTYQFAGVEDEKHKW 390

Query: 1304 KNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            K  + + L S PPDS++QQALITAI+DEDGYNW
Sbjct: 391  KYTDIEKLNSFPPDSEEQQALITAIQDEDGYNW 423


>gb|EMJ21470.1| hypothetical protein PRUPE_ppa000905mg [Prunus persica]
          Length = 965

 Score =  542 bits (1396), Expect = e-151
 Identities = 259/379 (68%), Positives = 305/379 (80%)
 Frame = +2

Query: 266  RTPTILCCFSAMPVEGPXXXXXXXXXQDSLFYSRAFWVSESIIAWNVEAGHGSCYLYASR 445
            R P + C  S++ +            Q SL YSRAFWVSESIIAWNV  G+GSCYL AS+
Sbjct: 59   RKPNLQCSASSLYMSAEESTSTS---QGSLLYSRAFWVSESIIAWNVYVGNGSCYLLASK 115

Query: 446  TADLSIADNEIQGYDVKIKLEGGVSGLPDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQL 625
            TA LS+  + I G DVK+KLE    GLP+ V +KFPHI DY AF+VPPDLD K LLKCQL
Sbjct: 116  TAALSLTSDGILGEDVKVKLEEDKHGLPENVKEKFPHIKDYRAFNVPPDLDAKPLLKCQL 175

Query: 626  AIALFSSEGNCTSATGLQLPGVLDELFSYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYK 805
            A+A F+S+G C+ ATGLQLPG+LD+LFSYNGPLGA++S ++V LYLWAPTAQ+V   +YK
Sbjct: 176  AVATFNSDGRCSDATGLQLPGILDDLFSYNGPLGALYSKQSVSLYLWAPTAQEVCVCIYK 235

Query: 806  NPSSDDPDEIVQLKELNGVWSAKGPRDWEGCYYVYEVFVYHPSTLKIERCIANDPYARGL 985
             PS   P E+VQL+E NGVWS KGP+ WEGCYYVYEV VYHPSTL+IE+C ANDPYARGL
Sbjct: 236  EPSGGSPLEVVQLEEFNGVWSTKGPKSWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGL 295

Query: 986  SADGRQTLLVNLDSDTSKPEGWDNLANEKPSIDSFSDISIYELHVRDFSANDSSVHPNLR 1165
            S+DGR+TLLVNLDSD  KPEGWD L +EKP I SFSDISIYELH+RDFSA+D +VHP  R
Sbjct: 296  SSDGRRTLLVNLDSDNIKPEGWDKLVDEKPDILSFSDISIYELHIRDFSASDQAVHPEFR 355

Query: 1166 GGYLAFTLQDSAGVLHLKRLSSAGITHVHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPD 1345
            GGYLAFTLQDSAG +HLK+LS+AGITHVHLLP FQFAGVDDEK  WK+V+ ++LE  P D
Sbjct: 356  GGYLAFTLQDSAGAIHLKKLSNAGITHVHLLPAFQFAGVDDEKENWKSVDPEVLEKFPSD 415

Query: 1346 SDQQQALITAIKDEDGYNW 1402
            SD+QQALITAI+++DGYNW
Sbjct: 416  SDKQQALITAIQNDDGYNW 434


>ref|XP_006378784.1| hypothetical protein POPTR_0010s23600g [Populus trichocarpa]
            gi|550330451|gb|ERP56581.1| hypothetical protein
            POPTR_0010s23600g [Populus trichocarpa]
          Length = 945

 Score =  527 bits (1357), Expect = e-147
 Identities = 259/384 (67%), Positives = 305/384 (79%), Gaps = 3/384 (0%)
 Frame = +2

Query: 260  TARTPTILCC--FSAMPVEGPXXXXXXXXXQDSLFYSRAFWVSESIIAWNVEAGH-GSCY 430
            T+ T +I C   FS+MP+E           QDSL YSRAFWV++SIIAWN +    GSCY
Sbjct: 62   TSPTRSIHCSSSFSSMPLE---VSTSPSQLQDSLLYSRAFWVTQSIIAWNADVVRDGSCY 118

Query: 431  LYASRTADLSIADNEIQGYDVKIKLEGGVSGLPDYVVKKFPHIGDYNAFSVPPDLDMKCL 610
            LYAS+TA LS+ D E++G+D KIKLE    G+P  V+ KFPH+ DY AF VP  +D K L
Sbjct: 119  LYASQTAALSVTDGEVEGHDFKIKLEEDSGGIPQNVIAKFPHVRDYKAFKVPSTVDAKSL 178

Query: 611  LKCQLAIALFSSEGNCTSATGLQLPGVLDELFSYNGPLGAVFSSEAVFLYLWAPTAQDVR 790
            +KCQLA+A F S+G C+ ATGLQL GVLDELF+Y+GPLGA +S +AV LYLWAPTAQ V 
Sbjct: 179  VKCQLAVATFGSDGKCSYATGLQLSGVLDELFAYDGPLGAHYSEDAVSLYLWAPTAQAVC 238

Query: 791  AFVYKNPSSDDPDEIVQLKELNGVWSAKGPRDWEGCYYVYEVFVYHPSTLKIERCIANDP 970
            A VYKN +S DP E+VQLKE+NGVWS +G +DWEGCYYVYEV VYHPSTL +E+C ANDP
Sbjct: 239  ACVYKNANSRDPVEVVQLKEVNGVWSVEGSKDWEGCYYVYEVSVYHPSTLHVEKCYANDP 298

Query: 971  YARGLSADGRQTLLVNLDSDTSKPEGWDNLANEKPSIDSFSDISIYELHVRDFSANDSSV 1150
            YARGLS D ++TL VNLDSDT KPEGW+ LA+EKP I SFSDISIYELHVRDFSAND +V
Sbjct: 299  YARGLSPDSQRTLFVNLDSDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSANDHTV 358

Query: 1151 HPNLRGGYLAFTLQDSAGVLHLKRLSSAGITHVHLLPTFQFAGVDDEKHKWKNVNFQLLE 1330
            HP+ +GGYLAFTL+DSAGVLHLK+LS+AGITHVHLLPTFQFAGVDD K  WK V+  +LE
Sbjct: 359  HPDFQGGYLAFTLEDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDVKENWKCVDSTVLE 418

Query: 1331 SLPPDSDQQQALITAIKDEDGYNW 1402
             LPPDS +QQA IT I+D+DGYNW
Sbjct: 419  KLPPDSTEQQAQITLIQDDDGYNW 442


>ref|XP_002315334.2| pullulanase family protein [Populus trichocarpa]
            gi|550330450|gb|EEF01505.2| pullulanase family protein
            [Populus trichocarpa]
          Length = 943

 Score =  527 bits (1357), Expect = e-147
 Identities = 259/384 (67%), Positives = 305/384 (79%), Gaps = 3/384 (0%)
 Frame = +2

Query: 260  TARTPTILCC--FSAMPVEGPXXXXXXXXXQDSLFYSRAFWVSESIIAWNVEAGH-GSCY 430
            T+ T +I C   FS+MP+E           QDSL YSRAFWV++SIIAWN +    GSCY
Sbjct: 62   TSPTRSIHCSSSFSSMPLE-----VSTSPSQDSLLYSRAFWVTQSIIAWNADVVRDGSCY 116

Query: 431  LYASRTADLSIADNEIQGYDVKIKLEGGVSGLPDYVVKKFPHIGDYNAFSVPPDLDMKCL 610
            LYAS+TA LS+ D E++G+D KIKLE    G+P  V+ KFPH+ DY AF VP  +D K L
Sbjct: 117  LYASQTAALSVTDGEVEGHDFKIKLEEDSGGIPQNVIAKFPHVRDYKAFKVPSTVDAKSL 176

Query: 611  LKCQLAIALFSSEGNCTSATGLQLPGVLDELFSYNGPLGAVFSSEAVFLYLWAPTAQDVR 790
            +KCQLA+A F S+G C+ ATGLQL GVLDELF+Y+GPLGA +S +AV LYLWAPTAQ V 
Sbjct: 177  VKCQLAVATFGSDGKCSYATGLQLSGVLDELFAYDGPLGAHYSEDAVSLYLWAPTAQAVC 236

Query: 791  AFVYKNPSSDDPDEIVQLKELNGVWSAKGPRDWEGCYYVYEVFVYHPSTLKIERCIANDP 970
            A VYKN +S DP E+VQLKE+NGVWS +G +DWEGCYYVYEV VYHPSTL +E+C ANDP
Sbjct: 237  ACVYKNANSRDPVEVVQLKEVNGVWSVEGSKDWEGCYYVYEVSVYHPSTLHVEKCYANDP 296

Query: 971  YARGLSADGRQTLLVNLDSDTSKPEGWDNLANEKPSIDSFSDISIYELHVRDFSANDSSV 1150
            YARGLS D ++TL VNLDSDT KPEGW+ LA+EKP I SFSDISIYELHVRDFSAND +V
Sbjct: 297  YARGLSPDSQRTLFVNLDSDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSANDHTV 356

Query: 1151 HPNLRGGYLAFTLQDSAGVLHLKRLSSAGITHVHLLPTFQFAGVDDEKHKWKNVNFQLLE 1330
            HP+ +GGYLAFTL+DSAGVLHLK+LS+AGITHVHLLPTFQFAGVDD K  WK V+  +LE
Sbjct: 357  HPDFQGGYLAFTLEDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDVKENWKCVDSTVLE 416

Query: 1331 SLPPDSDQQQALITAIKDEDGYNW 1402
             LPPDS +QQA IT I+D+DGYNW
Sbjct: 417  KLPPDSTEQQAQITLIQDDDGYNW 440


>gb|EOY18848.1| Limit dextrinase [Theobroma cacao]
          Length = 965

 Score =  525 bits (1352), Expect = e-146
 Identities = 252/373 (67%), Positives = 300/373 (80%)
 Frame = +2

Query: 284  CCFSAMPVEGPXXXXXXXXXQDSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSI 463
            C  S+MP +           QDSL YSRA+WVS++IIAWNV+ G GSCYLYAS+ A LS+
Sbjct: 60   CSSSSMPFQ----VSSSSQLQDSLLYSRAYWVSKTIIAWNVDVGDGSCYLYASKVAALSV 115

Query: 464  ADNEIQGYDVKIKLEGGVSGLPDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFS 643
             D+ IQG D +IKLE   +GLP  V++KFPHI +Y AF VPP LD K LLKCQLA+A F+
Sbjct: 116  TDDGIQGQDAEIKLEEDRNGLPPNVIEKFPHIQNYRAFKVPPALDAKNLLKCQLAVAAFN 175

Query: 644  SEGNCTSATGLQLPGVLDELFSYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDD 823
            S G C++ATGLQLPG+LDELFSY+GPLGA +  E V LYLWAPTAQ V A +YK+P    
Sbjct: 176  SHGKCSNATGLQLPGILDELFSYDGPLGAHYLGEVVSLYLWAPTAQAVHAHIYKDPMGGS 235

Query: 824  PDEIVQLKELNGVWSAKGPRDWEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQ 1003
            P EI+QL E NGVWS KGP+ WEGCYYVYEV VYHPSTL+IE+C ANDPYARGLS+DGR+
Sbjct: 236  PLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYANDPYARGLSSDGRR 295

Query: 1004 TLLVNLDSDTSKPEGWDNLANEKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAF 1183
            TL VNLDS+  KPEGWD LA++KP I SFSDISIYELH+RDFSAND++V+P+  GGY+AF
Sbjct: 296  TLFVNLDSNGLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDNTVNPDFCGGYMAF 355

Query: 1184 TLQDSAGVLHLKRLSSAGITHVHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQA 1363
            TLQDSAGVLHLK+LS+AGITHVHLLPTFQFAGVDDE   WK V++++L+ LPPDS  QQA
Sbjct: 356  TLQDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDESENWKYVDYKILKKLPPDSAGQQA 415

Query: 1364 LITAIKDEDGYNW 1402
             ITAI+++D YNW
Sbjct: 416  QITAIQNDDAYNW 428


>emb|CBI31395.3| unnamed protein product [Vitis vinifera]
          Length = 956

 Score =  524 bits (1350), Expect = e-146
 Identities = 255/377 (67%), Positives = 297/377 (78%)
 Frame = +2

Query: 272  PTILCCFSAMPVEGPXXXXXXXXXQDSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTA 451
            P   C    MP+E           QDSL YSRA+WVSESIIAWNV+ G GSCYLY+S+ A
Sbjct: 52   PISFCSCLPMPLE---VSTSTAQLQDSLLYSRAYWVSESIIAWNVDVGDGSCYLYSSKIA 108

Query: 452  DLSIADNEIQGYDVKIKLEGGVSGLPDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAI 631
             LSI D+ I G+D+ I+LE    GLP  V++KFP +  Y AF VP  +D K L+KCQLA+
Sbjct: 109  ALSIGDSGITGHDMTIQLEEDNGGLPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAV 168

Query: 632  ALFSSEGNCTSATGLQLPGVLDELFSYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNP 811
            A FS +G C + TGLQLPGVLDELFSY GPLGA+FS EAV LYLWAPTAQ V A +Y +P
Sbjct: 169  AAFSCDGQCNNVTGLQLPGVLDELFSYEGPLGAIFSKEAVTLYLWAPTAQVVHACIYGDP 228

Query: 812  SSDDPDEIVQLKELNGVWSAKGPRDWEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSA 991
            S  DP EIV L+E NGVWSA GP+ WEGCYY YEV VYHPSTL+IE+C ANDPYARGLSA
Sbjct: 229  SGGDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSA 288

Query: 992  DGRQTLLVNLDSDTSKPEGWDNLANEKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGG 1171
            D R+TLLVNLDSD  KPEGWDNLA+EKP++ SFSDISIYELH+RDFSA+D +VHP+ RGG
Sbjct: 289  DARRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDHTVHPDFRGG 348

Query: 1172 YLAFTLQDSAGVLHLKRLSSAGITHVHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSD 1351
            YLAFT QDSAG+LHLK+L +AGI+H+HLLPTFQFAGVDDEK KWK V+  +LE+LPPDS 
Sbjct: 349  YLAFTSQDSAGILHLKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPNILETLPPDSV 408

Query: 1352 QQQALITAIKDEDGYNW 1402
            +QQA I  I+DEDGYNW
Sbjct: 409  EQQAQIMTIQDEDGYNW 425


>ref|XP_002271820.2| PREDICTED: pullulanase 1, chloroplastic-like [Vitis vinifera]
          Length = 896

 Score =  523 bits (1347), Expect = e-146
 Identities = 250/353 (70%), Positives = 291/353 (82%)
 Frame = +2

Query: 344  QDSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSG 523
            QDSL YSRA+WVSESIIAWNV+ G GSCYLY+S+ A LSI D+ I G+D+ I+LE    G
Sbjct: 13   QDSLLYSRAYWVSESIIAWNVDVGDGSCYLYSSKIAALSIGDSGITGHDMTIQLEEDNGG 72

Query: 524  LPDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDEL 703
            LP  V++KFP +  Y AF VP  +D K L+KCQLA+A FS +G C + TGLQLPGVLDEL
Sbjct: 73   LPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNVTGLQLPGVLDEL 132

Query: 704  FSYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPR 883
            FSY GPLGA+FS EAV LYLWAPTAQ V A +Y +PS  DP EIV L+E NGVWSA GP+
Sbjct: 133  FSYEGPLGAIFSKEAVTLYLWAPTAQVVHACIYGDPSGGDPLEIVCLEEFNGVWSASGPK 192

Query: 884  DWEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLA 1063
             WEGCYY YEV VYHPSTL+IE+C ANDPYARGLSAD R+TLLVNLDSD  KPEGWDNLA
Sbjct: 193  SWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADARRTLLVNLDSDDLKPEGWDNLA 252

Query: 1064 NEKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGIT 1243
            +EKP++ SFSDISIYELH+RDFSA+D +VHP+ RGGYLAFT QDSAG+LHLK+L +AGI+
Sbjct: 253  DEKPALLSFSDISIYELHIRDFSASDHTVHPDFRGGYLAFTSQDSAGILHLKKLCNAGIS 312

Query: 1244 HVHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            H+HLLPTFQFAGVDDEK KWK V+  +LE+LPPDS +QQA I  I+DEDGYNW
Sbjct: 313  HLHLLPTFQFAGVDDEKDKWKCVDPNILETLPPDSVEQQAQIMTIQDEDGYNW 365


>ref|XP_002532780.1| pullulanase, putative [Ricinus communis] gi|223527468|gb|EEF29599.1|
            pullulanase, putative [Ricinus communis]
          Length = 964

 Score =  521 bits (1341), Expect = e-145
 Identities = 252/376 (67%), Positives = 304/376 (80%), Gaps = 1/376 (0%)
 Frame = +2

Query: 278  ILCCFSAMPVEGPXXXXXXXXXQDSLFYSRAFWVSESIIAWNVEAG-HGSCYLYASRTAD 454
            I C  S+MP++           QDSL YSRAFWVS++IIAWNV+ G +GSC+LYAS T  
Sbjct: 62   IRCSSSSMPLQ--ELSTSTSQFQDSLLYSRAFWVSKTIIAWNVDVGDNGSCFLYASDTGS 119

Query: 455  LSIADNEIQGYDVKIKLEGGVSGLPDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIA 634
            LS+++  IQG+DV++KLE    GLP+ VV KFPHI DY AF  PP LD K LLKCQLA+A
Sbjct: 120  LSVSNAGIQGHDVEVKLEKYNGGLPENVVVKFPHIRDYRAFKAPPTLDAKSLLKCQLAVA 179

Query: 635  LFSSEGNCTSATGLQLPGVLDELFSYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPS 814
             + ++G C SATGLQLPG+LDEL+SY+GPLGA +S  AV L+LWAPTAQ V  ++YK+  
Sbjct: 180  SYEADGKCRSATGLQLPGILDELYSYDGPLGAHYSKNAVSLHLWAPTAQAVLVYIYKDSF 239

Query: 815  SDDPDEIVQLKELNGVWSAKGPRDWEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSAD 994
            S  P EI QLKE+NGVWS KGP+DWEGCYYVYEV VYHPSTL+IE+C ANDPYARGLS+D
Sbjct: 240  SKVPLEIHQLKEVNGVWSIKGPKDWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSD 299

Query: 995  GRQTLLVNLDSDTSKPEGWDNLANEKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGY 1174
            G++TLLVNL S++ KPEGWDNLA EKPS+ SF DIS+YELH+RDFSAND +VHP+ RGGY
Sbjct: 300  GKRTLLVNLTSESLKPEGWDNLAKEKPSLLSFCDISLYELHIRDFSANDQTVHPDFRGGY 359

Query: 1175 LAFTLQDSAGVLHLKRLSSAGITHVHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQ 1354
            LAFT ++SAGVLHLK+LS+AGITHVHLLPTFQFAGVDD K  WK V+ ++LE+L PDS +
Sbjct: 360  LAFTFENSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDVKENWKCVDNKMLETLLPDSIE 419

Query: 1355 QQALITAIKDEDGYNW 1402
            QQA ITA++D DGYNW
Sbjct: 420  QQAQITAVQDNDGYNW 435


>ref|XP_006485313.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X1 [Citrus
            sinensis]
          Length = 969

 Score =  520 bits (1339), Expect = e-145
 Identities = 247/352 (70%), Positives = 291/352 (82%)
 Frame = +2

Query: 347  DSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSGL 526
            DSL YSRA+WVSESIIAWNV+   GSCYLYASRTA LSI+   IQG DV+I+L+    GL
Sbjct: 87   DSLLYSRAYWVSESIIAWNVDVPDGSCYLYASRTAALSISYGGIQGADVEIELQEDKGGL 146

Query: 527  PDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDELF 706
            P  V++KFPHI DY AF VP   D K LLKCQLA+  ++S+  C+ ATGLQLPG+LDELF
Sbjct: 147  PANVIEKFPHIRDYKAFKVPAGSDAKLLLKCQLAVGTYNSDRKCSDATGLQLPGILDELF 206

Query: 707  SYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPRD 886
            SY+GPLGA+++ E V LYLWAPTAQ V A +Y++P   +P E+VQLKE +GVWS KGP+ 
Sbjct: 207  SYDGPLGALYAEETVSLYLWAPTAQSVSACIYRDPLGGNPLEVVQLKENDGVWSIKGPKS 266

Query: 887  WEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLAN 1066
            WEGCYYVYEV VYHPS L+IE+C ANDPYARGLS+DGR+TLLVNLDSDT KPEGWD L  
Sbjct: 267  WEGCYYVYEVSVYHPSALQIEKCYANDPYARGLSSDGRRTLLVNLDSDTLKPEGWDKLVY 326

Query: 1067 EKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGITH 1246
            EKP I SFSDISIYELHVRDFS +D +VHP+ RGGYLAFTLQ+SAGV HLK+LS+AG+TH
Sbjct: 327  EKPDILSFSDISIYELHVRDFSVSDHTVHPDFRGGYLAFTLQNSAGVSHLKKLSNAGLTH 386

Query: 1247 VHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            VHLLPTFQFAGVDD K  WK+V+ ++LE LPPDS +QQA ITAI+++DGYNW
Sbjct: 387  VHLLPTFQFAGVDDRKENWKSVDAEVLEKLPPDSTEQQAQITAIQNDDGYNW 438


>ref|XP_006436527.1| hypothetical protein CICLE_v10031035mg [Citrus clementina]
            gi|557538723|gb|ESR49767.1| hypothetical protein
            CICLE_v10031035mg [Citrus clementina]
          Length = 590

 Score =  520 bits (1339), Expect = e-145
 Identities = 247/352 (70%), Positives = 291/352 (82%)
 Frame = +2

Query: 347  DSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSGL 526
            DSL YSRA+WVSESIIAWNV+   GSCYLYASRTA LSI+   IQG DV+I+L+    GL
Sbjct: 87   DSLLYSRAYWVSESIIAWNVDVPDGSCYLYASRTAALSISYGGIQGADVEIELQEDKGGL 146

Query: 527  PDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDELF 706
            P  V++KFPHI DY AF VP   D K LLKCQLA+  ++S+  C+ ATGLQLPG+LDELF
Sbjct: 147  PANVIEKFPHIRDYKAFKVPAGSDAKLLLKCQLAVGTYNSDRKCSDATGLQLPGILDELF 206

Query: 707  SYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPRD 886
            SY+GPLGA+++ E V LYLWAPTAQ V A +Y++P   +P E+VQLKE +GVWS KGP+ 
Sbjct: 207  SYDGPLGALYAEETVSLYLWAPTAQSVSACIYRDPLGGNPLEVVQLKENDGVWSIKGPKS 266

Query: 887  WEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLAN 1066
            WEGCYYVYEV VYHPS L+IE+C ANDPYARGLS+DGR+TLLVNLDSDT KPEGWD L  
Sbjct: 267  WEGCYYVYEVSVYHPSALQIEKCYANDPYARGLSSDGRRTLLVNLDSDTLKPEGWDKLVY 326

Query: 1067 EKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGITH 1246
            EKP I SFSDISIYELHVRDFS +D +VHP+ RGGYLAFTLQ+SAGV HLK+LS+AG+TH
Sbjct: 327  EKPDILSFSDISIYELHVRDFSVSDHTVHPDFRGGYLAFTLQNSAGVSHLKKLSNAGLTH 386

Query: 1247 VHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            VHLLPTFQFAGVDD K  WK+V+ ++LE LPPDS +QQA ITAI+++DGYNW
Sbjct: 387  VHLLPTFQFAGVDDRKENWKSVDAEVLEKLPPDSTEQQAQITAIQNDDGYNW 438


>ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic-like [Glycine max]
          Length = 951

 Score =  515 bits (1327), Expect = e-143
 Identities = 246/354 (69%), Positives = 297/354 (83%), Gaps = 1/354 (0%)
 Frame = +2

Query: 344  QDSLFYSRAFWVSESIIAWNVEAGHG-SCYLYASRTADLSIADNEIQGYDVKIKLEGGVS 520
            Q+ L YSRA+WV+ES+IAW+V+ G+G SCYL AS+ A L+IA+ +IQG D+KIKL+    
Sbjct: 67   QNGLLYSRAYWVAESLIAWDVDVGNGFSCYLLASKNASLTIANCQIQGEDLKIKLQEDRV 126

Query: 521  GLPDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDE 700
            GLP  VV+KFPHI  +  FS+PP LD+K LLK +LA+ +  S+G C + TGLQLPGVLD+
Sbjct: 127  GLPANVVEKFPHIRGHKVFSLPPTLDVKPLLKFRLAVVICDSDGECMNCTGLQLPGVLDD 186

Query: 701  LFSYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGP 880
            LFSY+GPLGA+FS EAV LYLWAPTAQ V A++YK+PS DDP EIV L+E NGVW  KGP
Sbjct: 187  LFSYSGPLGALFSEEAVSLYLWAPTAQAVHAYIYKDPSGDDPIEIVCLEEENGVWRTKGP 246

Query: 881  RDWEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNL 1060
            + WEGCYYVYEV VYHPST++IE+C  +DPYARGLS+DGR++ L+NLDS   KP+GWDNL
Sbjct: 247  KSWEGCYYVYEVCVYHPSTMRIEKCYTSDPYARGLSSDGRRSFLLNLDSVKLKPDGWDNL 306

Query: 1061 ANEKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGI 1240
            AN+KP+I SFSDISIYE+H+RDFSA+D SV P  RGGYLAFTLQDSAGVLHLK+LSSAGI
Sbjct: 307  ANKKPTIHSFSDISIYEMHIRDFSASDLSVQPEFRGGYLAFTLQDSAGVLHLKKLSSAGI 366

Query: 1241 THVHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            THVHLLPTFQFAGVDD+K  W+ V+  +LESLPPDSDQQQALITAI++ DGYNW
Sbjct: 367  THVHLLPTFQFAGVDDQKEDWRFVDTSILESLPPDSDQQQALITAIQNFDGYNW 420


>ref|XP_006485314.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X2 [Citrus
            sinensis]
          Length = 965

 Score =  513 bits (1321), Expect = e-143
 Identities = 247/352 (70%), Positives = 289/352 (82%)
 Frame = +2

Query: 347  DSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSGL 526
            DSL YSRA+WVSESIIAWNV+   GSCYLYASRTA LSI+   IQG DV+I+L+    GL
Sbjct: 87   DSLLYSRAYWVSESIIAWNVDVPDGSCYLYASRTAALSISYGGIQGADVEIELQEDKGGL 146

Query: 527  PDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDELF 706
            P  V++KFPHI DY AF VP   D K LLKCQLA+A    +  C+ ATGLQLPG+LDELF
Sbjct: 147  PANVIEKFPHIRDYKAFKVPAGSDAKLLLKCQLAVA----DRKCSDATGLQLPGILDELF 202

Query: 707  SYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPRD 886
            SY+GPLGA+++ E V LYLWAPTAQ V A +Y++P   +P E+VQLKE +GVWS KGP+ 
Sbjct: 203  SYDGPLGALYAEETVSLYLWAPTAQSVSACIYRDPLGGNPLEVVQLKENDGVWSIKGPKS 262

Query: 887  WEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLAN 1066
            WEGCYYVYEV VYHPS L+IE+C ANDPYARGLS+DGR+TLLVNLDSDT KPEGWD L  
Sbjct: 263  WEGCYYVYEVSVYHPSALQIEKCYANDPYARGLSSDGRRTLLVNLDSDTLKPEGWDKLVY 322

Query: 1067 EKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGITH 1246
            EKP I SFSDISIYELHVRDFS +D +VHP+ RGGYLAFTLQ+SAGV HLK+LS+AG+TH
Sbjct: 323  EKPDILSFSDISIYELHVRDFSVSDHTVHPDFRGGYLAFTLQNSAGVSHLKKLSNAGLTH 382

Query: 1247 VHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            VHLLPTFQFAGVDD K  WK+V+ ++LE LPPDS +QQA ITAI+++DGYNW
Sbjct: 383  VHLLPTFQFAGVDDRKENWKSVDAEVLEKLPPDSTEQQAQITAIQNDDGYNW 434


>ref|XP_004496127.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X1 [Cicer
            arietinum]
          Length = 953

 Score =  512 bits (1319), Expect = e-142
 Identities = 244/350 (69%), Positives = 285/350 (81%)
 Frame = +2

Query: 353  LFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSGLPD 532
            L YSRA+WVSE +IAWNV+  +G CYL +S+ A LSI++ +IQG D+KIKLE   +GL  
Sbjct: 73   LLYSRAYWVSEFVIAWNVDVENGFCYLLSSKYASLSISNGQIQGEDLKIKLEEDKAGLSA 132

Query: 533  YVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDELFSY 712
             VV+KFPHI  Y AF +PP  D+K  LK QLA+ +  S+  C + TGLQLPGVLDELFSY
Sbjct: 133  NVVEKFPHIRGYRAFRLPPGSDVKSFLKSQLAVVICDSDEKCRNCTGLQLPGVLDELFSY 192

Query: 713  NGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPRDWE 892
            NGPLGA+FS EAV LYLWAPTAQ V A++YK+PS DDP EIV L+E +GVW  KGP+ WE
Sbjct: 193  NGPLGALFSEEAVSLYLWAPTAQLVHAYIYKHPSGDDPIEIVPLEEEHGVWRTKGPKSWE 252

Query: 893  GCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLANEK 1072
            GCYYVYEV VYHPST ++E+C ANDPYARGLSADGR+T L++LDSD  KP+GWDNLANEK
Sbjct: 253  GCYYVYEVCVYHPSTSRVEKCYANDPYARGLSADGRRTFLLDLDSDELKPDGWDNLANEK 312

Query: 1073 PSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGITHVH 1252
            P + SFSDISIYE+H+RDFSAND SV    RGGYLAFT  DSAGVLHLK+LSSAGITHVH
Sbjct: 313  PILHSFSDISIYEMHIRDFSANDLSVQTEFRGGYLAFTFPDSAGVLHLKKLSSAGITHVH 372

Query: 1253 LLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            LLP FQFAGVDD+K  WKNV+  +LES PPDSDQQQALITAI++ DGYNW
Sbjct: 373  LLPAFQFAGVDDQKENWKNVDTSVLESFPPDSDQQQALITAIQNIDGYNW 422


>ref|XP_004307333.1| PREDICTED: pullulanase 1, chloroplastic-like [Fragaria vesca subsp.
            vesca]
          Length = 964

 Score =  511 bits (1316), Expect = e-142
 Identities = 241/353 (68%), Positives = 286/353 (81%)
 Frame = +2

Query: 344  QDSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSG 523
            + SL  SRAFWVSESIIAWNV+ G  SCYL+AS+TA LS+  + + G DVK++L     G
Sbjct: 81   EGSLLKSRAFWVSESIIAWNVDTGDDSCYLFASKTAALSLTGDGVSGEDVKVELREDRHG 140

Query: 524  LPDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDEL 703
            LP  V +KFPHI DY AF+VP DLD+K LLK QLA+A FS +G C+ ATGLQLPG+LDEL
Sbjct: 141  LPQNVKEKFPHIKDYRAFNVPSDLDVKSLLKAQLAVATFSCDGRCSDATGLQLPGILDEL 200

Query: 704  FSYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPR 883
            FSY GPLGAVFS EAV LYLWAPT+Q V   +YK P   +P EIVQL+E+NGVWS +GP+
Sbjct: 201  FSYEGPLGAVFSKEAVSLYLWAPTSQAVSVLIYKEPLGGNPLEIVQLEEVNGVWSTRGPK 260

Query: 884  DWEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLA 1063
             WEGCYYVYEV VYHPSTL+IE+C ANDPYARGLS+DGR+TL +NLDSD+ KP+GWD L 
Sbjct: 261  SWEGCYYVYEVSVYHPSTLQIEKCYANDPYARGLSSDGRRTLFINLDSDSIKPDGWDKLV 320

Query: 1064 NEKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGIT 1243
            +EKP I S SDISIYELH+RDFSAND +V P  RGGYLAFT QDSAGV HLK+LS AG+T
Sbjct: 321  DEKPDILSSSDISIYELHIRDFSANDQTVLPEFRGGYLAFTSQDSAGVSHLKKLSKAGMT 380

Query: 1244 HVHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            HVHLLP FQF+GVDDEK  WK+++ ++LE  P DS +QQALITAI+++DGYNW
Sbjct: 381  HVHLLPAFQFSGVDDEKENWKSIDSKILEKFPSDSSEQQALITAIQNDDGYNW 433


>ref|XP_004157562.1| PREDICTED: pullulanase 1, chloroplastic-like [Cucumis sativus]
          Length = 946

 Score =  495 bits (1274), Expect = e-137
 Identities = 228/353 (64%), Positives = 288/353 (81%)
 Frame = +2

Query: 344  QDSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSG 523
            +DSL YSRAFWVS+ +IAWNVE   GSCYL+AS+ A L + D  ++GYDVKI+LE   S 
Sbjct: 62   EDSLLYSRAFWVSKHVIAWNVEVESGSCYLFASKMATLRVEDGVVEGYDVKIRLEKDRSQ 121

Query: 524  LPDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDEL 703
            LP+ V+KKFPHI +Y  F+VPP  D++ LLKCQLA+A F+S G C + T LQLPGVLD+L
Sbjct: 122  LPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVATFNSYGECKNITCLQLPGVLDDL 181

Query: 704  FSYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPR 883
            FSY GPLGA++S EAV LYLWAPTAQ VRA ++++P    P E++ L+E++G+W  KGP+
Sbjct: 182  FSYEGPLGAIYSKEAVSLYLWAPTAQAVRAQIFRDPVGGMPFEVIPLEEVDGIWRTKGPK 241

Query: 884  DWEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLA 1063
             W+GCYY YEV VYHPSTL++E+C   DPY+RG+S+DGR+TL V+L SD   P+GWD LA
Sbjct: 242  SWKGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSDGRRTLFVDLFSDDLIPKGWDKLA 301

Query: 1064 NEKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGIT 1243
            +EKP +DSFSDISIYELHVRDFS +D SVHP+LRGGY+AFTLQDSAG+ HLK+LS+AG++
Sbjct: 302  DEKPPVDSFSDISIYELHVRDFSISDQSVHPDLRGGYMAFTLQDSAGINHLKKLSNAGLS 361

Query: 1244 HVHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            HVHLLPTFQF GVDD+K KWK V+ +LLE+LPPDS +QQ+LI  I++ DGYNW
Sbjct: 362  HVHLLPTFQFGGVDDDKTKWKFVDTELLENLPPDSAEQQSLIADIQNSDGYNW 414


>ref|XP_004142505.1| PREDICTED: pullulanase 1, chloroplastic-like [Cucumis sativus]
          Length = 960

 Score =  495 bits (1274), Expect = e-137
 Identities = 228/353 (64%), Positives = 288/353 (81%)
 Frame = +2

Query: 344  QDSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSG 523
            +DSL YSRAFWVS+ +IAWNVE   GSCYL+AS+ A L + D  ++GYDVKI+LE   S 
Sbjct: 76   EDSLLYSRAFWVSKHVIAWNVEVESGSCYLFASKMATLRVEDGVVEGYDVKIRLEKDRSQ 135

Query: 524  LPDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDEL 703
            LP+ V+KKFPHI +Y  F+VPP  D++ LLKCQLA+A F+S G C + T LQLPGVLD+L
Sbjct: 136  LPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVATFNSYGECKNITCLQLPGVLDDL 195

Query: 704  FSYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPR 883
            FSY GPLGA++S EAV LYLWAPTAQ VRA ++++P    P E++ L+E++G+W  KGP+
Sbjct: 196  FSYEGPLGAIYSKEAVSLYLWAPTAQAVRAQIFRDPVGGMPFEVIPLEEVDGIWRTKGPK 255

Query: 884  DWEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLA 1063
             W+GCYY YEV VYHPSTL++E+C   DPY+RG+S+DGR+TL V+L SD   P+GWD LA
Sbjct: 256  SWKGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSDGRRTLFVDLFSDDLIPKGWDKLA 315

Query: 1064 NEKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGIT 1243
            +EKP +DSFSDISIYELHVRDFS +D SVHP+LRGGY+AFTLQDSAG+ HLK+LS+AG++
Sbjct: 316  DEKPPVDSFSDISIYELHVRDFSISDQSVHPDLRGGYMAFTLQDSAGINHLKKLSNAGLS 375

Query: 1244 HVHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            HVHLLPTFQF GVDD+K KWK V+ +LLE+LPPDS +QQ+LI  I++ DGYNW
Sbjct: 376  HVHLLPTFQFGGVDDDKTKWKFVDTELLENLPPDSAEQQSLIADIQNSDGYNW 428


>ref|XP_006398895.1| hypothetical protein EUTSA_v10012585mg [Eutrema salsugineum]
            gi|557099985|gb|ESQ40348.1| hypothetical protein
            EUTSA_v10012585mg [Eutrema salsugineum]
          Length = 964

 Score =  479 bits (1234), Expect = e-133
 Identities = 225/352 (63%), Positives = 271/352 (76%)
 Frame = +2

Query: 347  DSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSGL 526
            D L YSRA+WV++ +IAWNV+ G GSCYLYAS  A LS +++ I GYD  +KLE     L
Sbjct: 82   DCLLYSRAYWVTQGVIAWNVDVGEGSCYLYASSIAGLSFSEDGIDGYDFSLKLEAESGSL 141

Query: 527  PDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDELF 706
            P  V++KFPHI +Y  F VP DLD++ L+K QLA+  F +EG     TGLQLPGVLDELF
Sbjct: 142  PTNVIEKFPHIRNYKPFKVPSDLDIRDLVKSQLAVVCFDAEGRLIQGTGLQLPGVLDELF 201

Query: 707  SYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPRD 886
            SY+GPLGA F+ E V L+LWAPT+Q V   +YKNP    P EI  LKE+NGVWS +GP  
Sbjct: 202  SYDGPLGAHFTPEGVSLHLWAPTSQAVSVCIYKNPLDKSPMEICPLKEVNGVWSTEGPSS 261

Query: 887  WEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLAN 1066
            WEGCYYVYEV VYHPSTLK+E C ANDPYARGLSADG +TLLVNL+SD  KPEGWD LA+
Sbjct: 262  WEGCYYVYEVSVYHPSTLKVETCYANDPYARGLSADGSKTLLVNLNSDDLKPEGWDKLAD 321

Query: 1067 EKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGITH 1246
             KP + S+SDISIYELHVRDFSAND +V P  RGGYLAFT +DSAGV HL++L+ AG+TH
Sbjct: 322  TKPCLRSYSDISIYELHVRDFSANDETVGPENRGGYLAFTFKDSAGVKHLQKLADAGLTH 381

Query: 1247 VHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            +HLLPT+QF  VDDEK  WK+++  LL+ LPPDS + QA IT I+++DGYNW
Sbjct: 382  LHLLPTYQFGDVDDEKENWKSIDSSLLKGLPPDSAEAQARITEIQNDDGYNW 433


>emb|CAA58803.1| pullulanase [Spinacia oleracea]
          Length = 964

 Score =  479 bits (1234), Expect = e-133
 Identities = 235/373 (63%), Positives = 287/373 (76%)
 Frame = +2

Query: 284  CCFSAMPVEGPXXXXXXXXXQDSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSI 463
            CC  A+ V G          Q SL   RA+W S+   AWNV+ G+GS YL+AS+TA L  
Sbjct: 62   CCSMAVEV-GSASSVSQSELQGSLNSCRAYWPSKYTFAWNVDIGNGSYYLFASKTAALKF 120

Query: 464  ADNEIQGYDVKIKLEGGVSGLPDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFS 643
             D  I+GYDVKIKL+    GLP  V +KFPHI  Y+AF  P  LD+  LLKCQLA+A FS
Sbjct: 121  TDAGIEGYDVKIKLDKDQGGLPANVTEKFPHIRGYSAFKAPATLDVDSLLKCQLAVAAFS 180

Query: 644  SEGNCTSATGLQLPGVLDELFSYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDD 823
            ++G C +ATGLQLPGV+DEL+SY+GPLGAVFS   + LYLWAPTAQ V A ++K+PS  +
Sbjct: 181  ADGACRNATGLQLPGVIDELYSYDGPLGAVFSENTISLYLWAPTAQAVSASIFKDPSGGE 240

Query: 824  PDEIVQLKELNGVWSAKGPRDWEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQ 1003
            P + VQL E NGVWSA GPR WEGCYYVYE+ VYH STL+IE+  A DPYARG+SAD ++
Sbjct: 241  PLQTVQLIESNGVWSAVGPRTWEGCYYVYEITVYHHSTLRIEKSFAIDPYARGISADVKR 300

Query: 1004 TLLVNLDSDTSKPEGWDNLANEKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAF 1183
            TLL +L S+T KPEGW+NLA+EKP + S SDIS+YELH+RDFSA D +VHP+LRGGYLAF
Sbjct: 301  TLLADLSSETLKPEGWENLADEKPHLLSPSDISLYELHIRDFSAYDLTVHPDLRGGYLAF 360

Query: 1184 TLQDSAGVLHLKRLSSAGITHVHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQA 1363
            T QDSAGV HL++LS+AG+THVHLLP+FQFA VDD+K KWK V+ +  E+LPPDS++QQA
Sbjct: 361  TSQDSAGVNHLEKLSAAGLTHVHLLPSFQFAEVDDDKKKWKFVDTKRFETLPPDSEEQQA 420

Query: 1364 LITAIKDEDGYNW 1402
             ITAI+DEDGYNW
Sbjct: 421  QITAIRDEDGYNW 433


>gb|AAO00771.1| pullulanase-like protein (starch debranching enzyme) [Arabidopsis
            thaliana]
          Length = 965

 Score =  479 bits (1233), Expect = e-132
 Identities = 227/352 (64%), Positives = 272/352 (77%)
 Frame = +2

Query: 347  DSLFYSRAFWVSESIIAWNVEAGHGSCYLYASRTADLSIADNEIQGYDVKIKLEGGVSGL 526
            D L YSRA+WV++ +IAWNV+ G GSCY YAS++A LS +++ I GYD++IKLE     L
Sbjct: 83   DCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSEDGIDGYDLRIKLEAESGSL 142

Query: 527  PDYVVKKFPHIGDYNAFSVPPDLDMKCLLKCQLAIALFSSEGNCTSATGLQLPGVLDELF 706
            P  V++KFPHI +Y +F VP DLD++ L+K QLA+  F +EG     TGLQLPGVLDELF
Sbjct: 143  PADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAEGRLIQGTGLQLPGVLDELF 202

Query: 707  SYNGPLGAVFSSEAVFLYLWAPTAQDVRAFVYKNPSSDDPDEIVQLKELNGVWSAKGPRD 886
            SY+GPLGA F+ E V L+LWAPTAQ V   +YKNP    P EI  LKE NGVWS +G   
Sbjct: 203  SYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPMEICPLKEANGVWSTEGACS 262

Query: 887  WEGCYYVYEVFVYHPSTLKIERCIANDPYARGLSADGRQTLLVNLDSDTSKPEGWDNLAN 1066
            W GCYYVY+V VYHPST+K+E C ANDPYARGLSADGR+T LVNLDSD  KPEGWDNLA+
Sbjct: 263  WGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTFLVNLDSDDLKPEGWDNLAD 322

Query: 1067 EKPSIDSFSDISIYELHVRDFSANDSSVHPNLRGGYLAFTLQDSAGVLHLKRLSSAGITH 1246
            +KP + SFSDISIYELHVRDFSAND +V P  RGGYLAFT +DSAGV HL++L  AG+TH
Sbjct: 323  KKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSKDSAGVKHLQKLVDAGLTH 382

Query: 1247 VHLLPTFQFAGVDDEKHKWKNVNFQLLESLPPDSDQQQALITAIKDEDGYNW 1402
            +HLLPTFQF  VDDEK  WK+V+  LLE L PDS + QA IT I+++DGYNW
Sbjct: 383  LHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNW 434