BLASTX nr result
ID: Rauwolfia21_contig00007900
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rauwolfia21_contig00007900
(183 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9SPP9.1|RG1_RAUSE RecName: Full=Raucaffricine-O-beta-D-gluco... 60 4e-13
pdb|4ATD|A Chain A, Crystal Structure Of Native Raucaffricine Gl... 60 4e-13
pdb|3U57|A Chain A, Structures Of Alkaloid Biosynthetic Glucosid... 60 4e-13
gb|AFI71457.1| scrictosidine-beta-D-glucosidase [Rauvolfia verti... 49 2e-06
ref|XP_003539381.1| PREDICTED: beta-glucosidase 12-like [Glycine... 46 2e-06
ref|XP_003597481.1| Beta-glucosidase D4 [Medicago truncatula] gi... 45 3e-06
ref|XP_003597482.1| Beta-glucosidase D4 [Medicago truncatula] gi... 44 4e-06
ref|XP_003597483.1| Beta-glucosidase D4 [Medicago truncatula] gi... 44 4e-06
ref|XP_004486932.1| PREDICTED: beta-glucosidase 12-like [Cicer a... 44 5e-06
ref|XP_003546992.2| PREDICTED: cyanogenic beta-glucosidase-like ... 44 5e-06
gb|ESW05681.1| hypothetical protein PHAVU_011G200700g, partial [... 44 5e-06
dbj|BAN58137.1| putative GH1 familly glucosidase [Ipomoea nil] 44 5e-06
sp|Q8GU20.1|SG1_RAUSE RecName: Full=Strictosidine-O-beta-D-gluco... 47 6e-06
ref|XP_006439048.1| hypothetical protein CICLE_v10031209mg [Citr... 49 8e-06
ref|XP_006439047.1| hypothetical protein CICLE_v10031209mg [Citr... 49 8e-06
ref|XP_003597509.1| Beta-glucosidase D2 [Medicago truncatula] gi... 43 8e-06
>sp|Q9SPP9.1|RG1_RAUSE RecName: Full=Raucaffricine-O-beta-D-glucosidase;
Short=Raucaffricine beta-glucosidase; Short=RsRG;
AltName: Full=Vomilenine glucosyltransferase;
Short=RsVGT gi|400977293|pdb|4A3Y|A Chain A, Crystal
Structure Of Raucaffricine Glucosidase From Ajmaline
Biosynthesis Pathway gi|400977294|pdb|4A3Y|B Chain B,
Crystal Structure Of Raucaffricine Glucosidase From
Ajmaline Biosynthesis Pathway gi|576864885|pdb|3ZJ6|A
Chain A, Crystal Of Raucaffricine Glucosidase In Complex
With Inhibitor gi|576864886|pdb|3ZJ6|B Chain B, Crystal
Of Raucaffricine Glucosidase In Complex With Inhibitor
gi|6103585|gb|AAF03675.1|AF149311_1
raucaffricine-O-beta-D-glucosidase [Rauvolfia
serpentina]
Length = 540
Score = 59.7 bits (143), Expect(3) = 4e-13
Identities = 26/31 (83%), Positives = 27/31 (87%)
Frame = +2
Query: 89 MRQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
MR YLQDH+FNVRQAM DGVNVKGYF WSL
Sbjct: 441 MRLKYLQDHIFNVRQAMNDGVNVKGYFAWSL 471
Score = 36.6 bits (83), Expect(3) = 4e-13
Identities = 15/16 (93%), Positives = 16/16 (100%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
KKTYNVPLIY+TENGV
Sbjct: 408 KKTYNVPLIYVTENGV 423
Score = 23.1 bits (48), Expect(3) = 4e-13
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +1
Query: 22 LSYISQRMEFDIQNTNLTLKLA 87
L Y+++ D++NTNLTL A
Sbjct: 415 LIYVTENGVDDVKNTNLTLSEA 436
>pdb|4ATD|A Chain A, Crystal Structure Of Native Raucaffricine Glucosidase
gi|442570519|pdb|4ATD|B Chain B, Crystal Structure Of
Native Raucaffricine Glucosidase gi|444302131|pdb|4ATL|A
Chain A, Crystal Structure Of Raucaffricine Glucosidase
In Complex With Glucose gi|444302132|pdb|4ATL|B Chain B,
Crystal Structure Of Raucaffricine Glucosidase In
Complex With Glucose
Length = 513
Score = 59.7 bits (143), Expect(3) = 4e-13
Identities = 26/31 (83%), Positives = 27/31 (87%)
Frame = +2
Query: 89 MRQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
MR YLQDH+FNVRQAM DGVNVKGYF WSL
Sbjct: 441 MRLKYLQDHIFNVRQAMNDGVNVKGYFAWSL 471
Score = 36.6 bits (83), Expect(3) = 4e-13
Identities = 15/16 (93%), Positives = 16/16 (100%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
KKTYNVPLIY+TENGV
Sbjct: 408 KKTYNVPLIYVTENGV 423
Score = 23.1 bits (48), Expect(3) = 4e-13
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +1
Query: 22 LSYISQRMEFDIQNTNLTLKLA 87
L Y+++ D++NTNLTL A
Sbjct: 415 LIYVTENGVDDVKNTNLTLSEA 436
>pdb|3U57|A Chain A, Structures Of Alkaloid Biosynthetic Glucosidases Decode
Substrate Specificity gi|358439929|pdb|3U57|B Chain B,
Structures Of Alkaloid Biosynthetic Glucosidases Decode
Substrate Specificity gi|358439930|pdb|3U5U|A Chain A,
Structures Of Alkaloid Biosynthetic Glucosidases Decode
Substrate Specificity gi|358439931|pdb|3U5U|B Chain B,
Structures Of Alkaloid Biosynthetic Glucosidases Decode
Substrate Specificity gi|358439932|pdb|3U5Y|A Chain A,
Structures Of Alkaloid Biosynthetic Glucosidases Decode
Substrate Specificity gi|358439933|pdb|3U5Y|B Chain B,
Structures Of Alkaloid Biosynthetic Glucosidases Decode
Substrate Specificity gi|451928645|pdb|4EK7|A Chain A,
High Speed X-ray Analysis Of Plant Enzymes At Room
Temperature gi|451928646|pdb|4EK7|B Chain B, High Speed
X-ray Analysis Of Plant Enzymes At Room Temperature
Length = 513
Score = 59.7 bits (143), Expect(3) = 4e-13
Identities = 26/31 (83%), Positives = 27/31 (87%)
Frame = +2
Query: 89 MRQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
MR YLQDH+FNVRQAM DGVNVKGYF WSL
Sbjct: 441 MRLKYLQDHIFNVRQAMNDGVNVKGYFAWSL 471
Score = 36.6 bits (83), Expect(3) = 4e-13
Identities = 15/16 (93%), Positives = 16/16 (100%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
KKTYNVPLIY+TENGV
Sbjct: 408 KKTYNVPLIYVTENGV 423
Score = 23.1 bits (48), Expect(3) = 4e-13
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +1
Query: 22 LSYISQRMEFDIQNTNLTLKLA 87
L Y+++ D++NTNLTL A
Sbjct: 415 LIYVTENGVDDVKNTNLTLSEA 436
>gb|AFI71457.1| scrictosidine-beta-D-glucosidase [Rauvolfia verticillata]
Length = 536
Score = 49.3 bits (116), Expect(2) = 2e-06
Identities = 22/31 (70%), Positives = 24/31 (77%)
Frame = +2
Query: 86 PMRQDYLQDHLFNVRQAMTDGVNVKGYFVWS 178
P R DY Q HL +VR A+ DGVNVKGYFVWS
Sbjct: 439 PERTDYHQKHLASVRDAIDDGVNVKGYFVWS 469
Score = 28.1 bits (61), Expect(2) = 2e-06
Identities = 9/16 (56%), Positives = 16/16 (100%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
K+TY+VP++Y+TE+G+
Sbjct: 407 KETYHVPVLYVTESGM 422
>ref|XP_003539381.1| PREDICTED: beta-glucosidase 12-like [Glycine max]
Length = 516
Score = 46.2 bits (108), Expect(2) = 2e-06
Identities = 20/30 (66%), Positives = 24/30 (80%)
Frame = +2
Query: 92 RQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
R DYL HL+ ++ A+ DGVNVKGYFVWSL
Sbjct: 444 RVDYLYRHLYYLQTAIKDGVNVKGYFVWSL 473
Score = 30.8 bits (68), Expect(2) = 2e-06
Identities = 15/21 (71%), Positives = 16/21 (76%)
Frame = +3
Query: 3 KKTYNVPLIYITENGVRYTEY 65
KK YN PLIYITENG Y E+
Sbjct: 410 KKQYNNPLIYITENG--YDEF 428
>ref|XP_003597481.1| Beta-glucosidase D4 [Medicago truncatula]
gi|355486529|gb|AES67732.1| Beta-glucosidase D4
[Medicago truncatula]
Length = 505
Score = 44.7 bits (104), Expect(2) = 3e-06
Identities = 19/30 (63%), Positives = 22/30 (73%)
Frame = +2
Query: 92 RQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
R DY HLF +R A+ +GVNVKGYF WSL
Sbjct: 433 RIDYYHRHLFYIRSAIKNGVNVKGYFAWSL 462
Score = 32.0 bits (71), Expect(2) = 3e-06
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
KK YN PLIYITENG+
Sbjct: 399 KKVYNNPLIYITENGI 414
>ref|XP_003597482.1| Beta-glucosidase D4 [Medicago truncatula]
gi|355486530|gb|AES67733.1| Beta-glucosidase D4
[Medicago truncatula]
Length = 508
Score = 44.3 bits (103), Expect(2) = 4e-06
Identities = 18/30 (60%), Positives = 22/30 (73%)
Frame = +2
Query: 92 RQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
R DY HLF +R A+ +GVN+KGYF WSL
Sbjct: 436 RIDYYHRHLFYIRSAIKNGVNIKGYFAWSL 465
Score = 32.0 bits (71), Expect(2) = 4e-06
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
KK YN PLIYITENG+
Sbjct: 402 KKVYNNPLIYITENGI 417
>ref|XP_003597483.1| Beta-glucosidase D4 [Medicago truncatula]
gi|355486531|gb|AES67734.1| Beta-glucosidase D4
[Medicago truncatula]
Length = 460
Score = 44.3 bits (103), Expect(2) = 4e-06
Identities = 18/30 (60%), Positives = 22/30 (73%)
Frame = +2
Query: 92 RQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
R DY HLF +R A+ +GVN+KGYF WSL
Sbjct: 388 RIDYYHRHLFYIRSAIKNGVNIKGYFAWSL 417
Score = 32.0 bits (71), Expect(2) = 4e-06
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
KK YN PLIYITENG+
Sbjct: 354 KKVYNNPLIYITENGI 369
>ref|XP_004486932.1| PREDICTED: beta-glucosidase 12-like [Cicer arietinum]
Length = 514
Score = 43.9 bits (102), Expect(2) = 5e-06
Identities = 18/30 (60%), Positives = 22/30 (73%)
Frame = +2
Query: 92 RQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
R DY HLF +R A+ +GVN+KGYF WSL
Sbjct: 442 RIDYYYRHLFYIRSAIRNGVNIKGYFAWSL 471
Score = 32.0 bits (71), Expect(2) = 5e-06
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
KK YN PLIYITENGV
Sbjct: 408 KKKYNNPLIYITENGV 423
>ref|XP_003546992.2| PREDICTED: cyanogenic beta-glucosidase-like isoform X1 [Glycine
max]
Length = 513
Score = 44.3 bits (103), Expect(2) = 5e-06
Identities = 19/30 (63%), Positives = 22/30 (73%)
Frame = +2
Query: 92 RQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
R DY HLF ++ A+ DG NVKGYFVWSL
Sbjct: 441 RIDYYYRHLFYLKSAIKDGANVKGYFVWSL 470
Score = 31.6 bits (70), Expect(2) = 5e-06
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
KK YN PLIYITENG+
Sbjct: 407 KKKYNNPLIYITENGI 422
>gb|ESW05681.1| hypothetical protein PHAVU_011G200700g, partial [Phaseolus
vulgaris]
Length = 334
Score = 44.3 bits (103), Expect(2) = 5e-06
Identities = 18/30 (60%), Positives = 23/30 (76%)
Frame = +2
Query: 92 RQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
R DY DHL+ ++ A+ +GVNVKGYF WSL
Sbjct: 289 RIDYFYDHLYYLQSAIENGVNVKGYFAWSL 318
Score = 31.6 bits (70), Expect(2) = 5e-06
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
KK YN PLIYITENG+
Sbjct: 255 KKKYNNPLIYITENGI 270
>dbj|BAN58137.1| putative GH1 familly glucosidase [Ipomoea nil]
Length = 527
Score = 43.9 bits (102), Expect(3) = 5e-06
Identities = 17/30 (56%), Positives = 25/30 (83%)
Frame = +2
Query: 92 RQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
R +Y ++H+F + +A+ +GVNVKGYFVWSL
Sbjct: 453 RLNYHREHIFYISKAINEGVNVKGYFVWSL 482
Score = 30.4 bits (67), Expect(3) = 5e-06
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = +3
Query: 3 KKTYNVPLIYITE 41
KKTYN+PLIYITE
Sbjct: 419 KKTYNIPLIYITE 431
Score = 20.4 bits (41), Expect(3) = 5e-06
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +1
Query: 22 LSYISQRMEFDIQNTNLTLKLA 87
L YI++++ N+NLTL A
Sbjct: 426 LIYITEKVMMKSNNSNLTLSEA 447
>sp|Q8GU20.1|SG1_RAUSE RecName: Full=Strictosidine-O-beta-D-glucosidase
gi|167013222|pdb|2JF6|A Chain A, Structure Of Inactive
Mutant Of Strictosidine Glucosidase In Complex With
Strictosidine gi|167013223|pdb|2JF6|B Chain B, Structure
Of Inactive Mutant Of Strictosidine Glucosidase In
Complex With Strictosidine gi|167013224|pdb|2JF7|A Chain
A, Structure Of Strictosidine Glucosidase
gi|167013225|pdb|2JF7|B Chain B, Structure Of
Strictosidine Glucosidase gi|582044995|pdb|3ZJ7|A Chain
A, Crystal Structure Of Strictosidine Glucosidase In
Complex With Inhibitor-1 gi|582044996|pdb|3ZJ7|B Chain
B, Crystal Structure Of Strictosidine Glucosidase In
Complex With Inhibitor-1 gi|587759589|pdb|3ZJ8|A Chain
A, Crystal Structure Of Strictosidine Glucosidase In
Complex With Inhibitor-2 gi|587759590|pdb|3ZJ8|B Chain
B, Crystal Structure Of Strictosidine Glucosidase In
Complex With Inhibitor-2 gi|27527664|emb|CAC83098.1|
strictosidine-O-beta-D-glucosidase [Rauvolfia
serpentina]
Length = 532
Score = 47.4 bits (111), Expect(2) = 6e-06
Identities = 21/29 (72%), Positives = 23/29 (79%)
Frame = +2
Query: 92 RQDYLQDHLFNVRQAMTDGVNVKGYFVWS 178
R DY Q HL +VR A+ DGVNVKGYFVWS
Sbjct: 438 RTDYHQKHLASVRDAIDDGVNVKGYFVWS 466
Score = 28.1 bits (61), Expect(2) = 6e-06
Identities = 9/16 (56%), Positives = 16/16 (100%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
K+TY+VP++Y+TE+G+
Sbjct: 404 KETYHVPVLYVTESGM 419
>ref|XP_006439048.1| hypothetical protein CICLE_v10031209mg [Citrus clementina]
gi|557541244|gb|ESR52288.1| hypothetical protein
CICLE_v10031209mg [Citrus clementina]
Length = 530
Score = 48.9 bits (115), Expect(2) = 8e-06
Identities = 20/32 (62%), Positives = 26/32 (81%)
Frame = +2
Query: 86 PMRQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
PMR DY ++HL +R+A+ +GVNVKGYF WSL
Sbjct: 457 PMRLDYFKNHLLFLRKAIKEGVNVKGYFAWSL 488
Score = 26.2 bits (56), Expect(2) = 8e-06
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3
Query: 3 KKTYNVPLIYITENG 47
K+ YN P +YITENG
Sbjct: 425 KEKYNNPTLYITENG 439
>ref|XP_006439047.1| hypothetical protein CICLE_v10031209mg [Citrus clementina]
gi|568858588|ref|XP_006482831.1| PREDICTED:
beta-glucosidase 13-like [Citrus sinensis]
gi|557541243|gb|ESR52287.1| hypothetical protein
CICLE_v10031209mg [Citrus clementina]
Length = 517
Score = 48.9 bits (115), Expect(2) = 8e-06
Identities = 20/32 (62%), Positives = 26/32 (81%)
Frame = +2
Query: 86 PMRQDYLQDHLFNVRQAMTDGVNVKGYFVWSL 181
PMR DY ++HL +R+A+ +GVNVKGYF WSL
Sbjct: 444 PMRLDYFKNHLLFLRKAIKEGVNVKGYFAWSL 475
Score = 26.2 bits (56), Expect(2) = 8e-06
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3
Query: 3 KKTYNVPLIYITENG 47
K+ YN P +YITENG
Sbjct: 412 KEKYNNPTLYITENG 426
>ref|XP_003597509.1| Beta-glucosidase D2 [Medicago truncatula]
gi|355486557|gb|AES67760.1| Beta-glucosidase D2
[Medicago truncatula]
Length = 515
Score = 42.7 bits (99), Expect(2) = 8e-06
Identities = 17/29 (58%), Positives = 21/29 (72%)
Frame = +2
Query: 92 RQDYLQDHLFNVRQAMTDGVNVKGYFVWS 178
R DY HL+ +R A+ DGVN+KGYF WS
Sbjct: 443 RIDYFHRHLYYIRCAIKDGVNIKGYFAWS 471
Score = 32.3 bits (72), Expect(2) = 8e-06
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = +3
Query: 3 KKTYNVPLIYITENGV 50
KK YN PLIYITENGV
Sbjct: 409 KKVYNNPLIYITENGV 424