BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Perilla23_contig00022758
         (1263 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011082168.1| PREDICTED: uncharacterized protein LOC105164...   250   2e-63
ref|XP_012837335.1| PREDICTED: uncharacterized protein LOC105957...   233   2e-58
ref|XP_012837333.1| PREDICTED: uncharacterized protein LOC105957...   233   2e-58
ref|XP_012837332.1| PREDICTED: uncharacterized protein LOC105957...   233   2e-58
gb|EYU37440.1| hypothetical protein MIMGU_mgv11b003028mg [Erythr...   233   2e-58
ref|XP_002314010.1| las1-like family protein [Populus trichocarp...   176   5e-41
ref|XP_011036402.1| PREDICTED: uncharacterized protein LOC105133...   171   9e-40
ref|XP_010656891.1| PREDICTED: pre-rRNA-processing protein las1 ...   170   2e-39
emb|CBI17902.3| unnamed protein product [Vitis vinifera]              170   2e-39
ref|XP_008227626.1| PREDICTED: uncharacterized protein LOC103327...   165   9e-38
ref|XP_002298459.2| las1-like family protein [Populus trichocarp...   159   4e-36
gb|ABK93614.1| unknown [Populus trichocarpa]                          159   4e-36
ref|XP_007198899.1| hypothetical protein PRUPE_ppa003025mg [Prun...   159   6e-36
ref|XP_010097924.1| hypothetical protein L484_009358 [Morus nota...   157   2e-35
ref|XP_011464875.1| PREDICTED: protein LAS1 isoform X2 [Fragaria...   157   2e-35
ref|XP_004299975.1| PREDICTED: uncharacterized protein LOC101296...   157   2e-35
ref|XP_007214532.1| hypothetical protein PRUPE_ppa003033mg [Prun...   157   2e-35
ref|XP_008449642.1| PREDICTED: pre-rRNA-processing protein las1 ...   156   3e-35
ref|XP_009353741.1| PREDICTED: uncharacterized protein LOC103944...   156   4e-35
ref|XP_009353740.1| PREDICTED: uncharacterized protein LOC103944...   156   4e-35

>ref|XP_011082168.1| PREDICTED: uncharacterized protein LOC105164994 [Sesamum indicum]
          Length = 626

 Score =  250 bits (638), Expect = 2e-63
 Identities = 147/280 (52%), Positives = 188/280 (67%), Gaps = 9/280 (3%)
 Frame = -2

Query: 1175 YQSPLDSVKMRRFELLSHLFGWLVENLKTLNPVGSKESAETKG------LPRATXXXXXX 1014
            +QSP +S    RFE LS+LF WL  NLKTL PV  KESAE+K       L +AT      
Sbjct: 350  HQSPENSTNTHRFESLSYLFEWLTGNLKTLYPVRQKESAESKDSKTEKHLQKATVLGFLR 409

Query: 1013 XXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSVSEPNSISAYDDTLLSQ 834
                   PGN++LM +A+TLAQ +G  SL+ KLKKLALL+L S SE NS+ + ++T+LS 
Sbjct: 410  RCLLVSPPGNRKLMGAALTLAQQTGDRSLLHKLKKLALLDL-STSEANSVYSSNETILSG 468

Query: 833  QERSIRQAEGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTIGFS 654
            +E SIRQAE K ELI++ RMQ KH  P  + A  TKS+W+VAKSW PCPIGMLP T+GFS
Sbjct: 469  EEYSIRQAEEKFELIRQFRMQSKHIKPKQS-AMETKSKWAVAKSWNPCPIGMLPHTVGFS 527

Query: 653  GRLPTLDCVDNRREVTTASDIKEVSKQSHR--KREAEISTQELDVTPVKKV-EMEADCSM 483
            GRLP LD V+   E  T SD KE ++Q +R  KR+AE   +E   T VKK+ E E D   
Sbjct: 528  GRLPVLDRVNECTEAATLSDSKE-NRQLNRCGKRDAETDIEEKANTSVKKMKETEEDHVP 586

Query: 482  QNRSDSSAEDIEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
              + D S++ ++GHLMIDG WK+VGE+ELL + SAV+LL+
Sbjct: 587  HYQGDISSQGVKGHLMIDGVWKEVGEEELLALTSAVRLLV 626


>ref|XP_012837335.1| PREDICTED: uncharacterized protein LOC105957904 isoform X3
            [Erythranthe guttatus]
          Length = 557

 Score =  233 bits (595), Expect = 2e-58
 Identities = 144/284 (50%), Positives = 188/284 (66%), Gaps = 12/284 (4%)
 Frame = -2

Query: 1178 DYQSPLDSVKMRRFELLSHLFGWLVENLKTLNPVGSK-ESAETKG------LPRATXXXX 1020
            D+QSP +S K  R ELL  LF WLV+NLKT  PV  K ESAE K       LP+ T    
Sbjct: 279  DHQSPDNSTKPHRNELLPCLFEWLVQNLKTSYPVSKKKESAEIKSSKTEKYLPKETVLGL 338

Query: 1019 XXXXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSVSEPNSISAYDDTLL 840
                     PG  +LM+SAVTLAQLSG ++L+QKLKK A+LE  S+SE N+  +  +TL 
Sbjct: 339  VRRCLLLSYPGKNRLMSSAVTLAQLSGDATLLQKLKKFAILEQLSISEANTEISCTETLF 398

Query: 839  SQQERSIRQAEGKLELIKRQ-RMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTI 663
            S+QE SIR+AE K EL+ RQ RMQ+K+  P L+E     SRWSV KSW  CP+GMLP ++
Sbjct: 399  SEQEYSIRKAEEKFELMMRQHRMQKKNEKPNLSE-----SRWSVIKSWNSCPMGMLPHSV 453

Query: 662  GFSGRLPTLDCVDN-RREVTTASDIK-EVSKQSHRKREAEISTQELDVTPVKKVEMEA-D 492
            GFSGR  ++  +D+   EV T+SD K +    +  KREAEI T E+D T VK++E+ A D
Sbjct: 454  GFSGRFSSVGLIDDVCEEVVTSSDNKGDCVVNNLGKREAEICTAEMDDTRVKRLEIVAED 513

Query: 491  CSMQNRSDSSAED-IEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            C MQ++ D S E+  +G L+IDG WKKVGE+EL  IASA+++L+
Sbjct: 514  CVMQDQDDVSEEEGTKGCLVIDGVWKKVGEEELSAIASAIRVLV 557


>ref|XP_012837333.1| PREDICTED: uncharacterized protein LOC105957904 isoform X2
            [Erythranthe guttatus]
          Length = 604

 Score =  233 bits (595), Expect = 2e-58
 Identities = 144/284 (50%), Positives = 188/284 (66%), Gaps = 12/284 (4%)
 Frame = -2

Query: 1178 DYQSPLDSVKMRRFELLSHLFGWLVENLKTLNPVGSK-ESAETKG------LPRATXXXX 1020
            D+QSP +S K  R ELL  LF WLV+NLKT  PV  K ESAE K       LP+ T    
Sbjct: 326  DHQSPDNSTKPHRNELLPCLFEWLVQNLKTSYPVSKKKESAEIKSSKTEKYLPKETVLGL 385

Query: 1019 XXXXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSVSEPNSISAYDDTLL 840
                     PG  +LM+SAVTLAQLSG ++L+QKLKK A+LE  S+SE N+  +  +TL 
Sbjct: 386  VRRCLLLSYPGKNRLMSSAVTLAQLSGDATLLQKLKKFAILEQLSISEANTEISCTETLF 445

Query: 839  SQQERSIRQAEGKLELIKRQ-RMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTI 663
            S+QE SIR+AE K EL+ RQ RMQ+K+  P L+E     SRWSV KSW  CP+GMLP ++
Sbjct: 446  SEQEYSIRKAEEKFELMMRQHRMQKKNEKPNLSE-----SRWSVIKSWNSCPMGMLPHSV 500

Query: 662  GFSGRLPTLDCVDN-RREVTTASDIK-EVSKQSHRKREAEISTQELDVTPVKKVEMEA-D 492
            GFSGR  ++  +D+   EV T+SD K +    +  KREAEI T E+D T VK++E+ A D
Sbjct: 501  GFSGRFSSVGLIDDVCEEVVTSSDNKGDCVVNNLGKREAEICTAEMDDTRVKRLEIVAED 560

Query: 491  CSMQNRSDSSAED-IEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            C MQ++ D S E+  +G L+IDG WKKVGE+EL  IASA+++L+
Sbjct: 561  CVMQDQDDVSEEEGTKGCLVIDGVWKKVGEEELSAIASAIRVLV 604


>ref|XP_012837332.1| PREDICTED: uncharacterized protein LOC105957904 isoform X1
            [Erythranthe guttatus]
          Length = 622

 Score =  233 bits (595), Expect = 2e-58
 Identities = 144/284 (50%), Positives = 188/284 (66%), Gaps = 12/284 (4%)
 Frame = -2

Query: 1178 DYQSPLDSVKMRRFELLSHLFGWLVENLKTLNPVGSK-ESAETKG------LPRATXXXX 1020
            D+QSP +S K  R ELL  LF WLV+NLKT  PV  K ESAE K       LP+ T    
Sbjct: 344  DHQSPDNSTKPHRNELLPCLFEWLVQNLKTSYPVSKKKESAEIKSSKTEKYLPKETVLGL 403

Query: 1019 XXXXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSVSEPNSISAYDDTLL 840
                     PG  +LM+SAVTLAQLSG ++L+QKLKK A+LE  S+SE N+  +  +TL 
Sbjct: 404  VRRCLLLSYPGKNRLMSSAVTLAQLSGDATLLQKLKKFAILEQLSISEANTEISCTETLF 463

Query: 839  SQQERSIRQAEGKLELIKRQ-RMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTI 663
            S+QE SIR+AE K EL+ RQ RMQ+K+  P L+E     SRWSV KSW  CP+GMLP ++
Sbjct: 464  SEQEYSIRKAEEKFELMMRQHRMQKKNEKPNLSE-----SRWSVIKSWNSCPMGMLPHSV 518

Query: 662  GFSGRLPTLDCVDN-RREVTTASDIK-EVSKQSHRKREAEISTQELDVTPVKKVEMEA-D 492
            GFSGR  ++  +D+   EV T+SD K +    +  KREAEI T E+D T VK++E+ A D
Sbjct: 519  GFSGRFSSVGLIDDVCEEVVTSSDNKGDCVVNNLGKREAEICTAEMDDTRVKRLEIVAED 578

Query: 491  CSMQNRSDSSAED-IEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            C MQ++ D S E+  +G L+IDG WKKVGE+EL  IASA+++L+
Sbjct: 579  CVMQDQDDVSEEEGTKGCLVIDGVWKKVGEEELSAIASAIRVLV 622


>gb|EYU37440.1| hypothetical protein MIMGU_mgv11b003028mg [Erythranthe guttata]
          Length = 571

 Score =  233 bits (595), Expect = 2e-58
 Identities = 144/284 (50%), Positives = 188/284 (66%), Gaps = 12/284 (4%)
 Frame = -2

Query: 1178 DYQSPLDSVKMRRFELLSHLFGWLVENLKTLNPVGSK-ESAETKG------LPRATXXXX 1020
            D+QSP +S K  R ELL  LF WLV+NLKT  PV  K ESAE K       LP+ T    
Sbjct: 293  DHQSPDNSTKPHRNELLPCLFEWLVQNLKTSYPVSKKKESAEIKSSKTEKYLPKETVLGL 352

Query: 1019 XXXXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSVSEPNSISAYDDTLL 840
                     PG  +LM+SAVTLAQLSG ++L+QKLKK A+LE  S+SE N+  +  +TL 
Sbjct: 353  VRRCLLLSYPGKNRLMSSAVTLAQLSGDATLLQKLKKFAILEQLSISEANTEISCTETLF 412

Query: 839  SQQERSIRQAEGKLELIKRQ-RMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTI 663
            S+QE SIR+AE K EL+ RQ RMQ+K+  P L+E     SRWSV KSW  CP+GMLP ++
Sbjct: 413  SEQEYSIRKAEEKFELMMRQHRMQKKNEKPNLSE-----SRWSVIKSWNSCPMGMLPHSV 467

Query: 662  GFSGRLPTLDCVDN-RREVTTASDIK-EVSKQSHRKREAEISTQELDVTPVKKVEMEA-D 492
            GFSGR  ++  +D+   EV T+SD K +    +  KREAEI T E+D T VK++E+ A D
Sbjct: 468  GFSGRFSSVGLIDDVCEEVVTSSDNKGDCVVNNLGKREAEICTAEMDDTRVKRLEIVAED 527

Query: 491  CSMQNRSDSSAED-IEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            C MQ++ D S E+  +G L+IDG WKKVGE+EL  IASA+++L+
Sbjct: 528  CVMQDQDDVSEEEGTKGCLVIDGVWKKVGEEELSAIASAIRVLV 571


>ref|XP_002314010.1| las1-like family protein [Populus trichocarpa]
            gi|222850418|gb|EEE87965.1| las1-like family protein
            [Populus trichocarpa]
          Length = 617

 Score =  176 bits (445), Expect = 5e-41
 Identities = 116/281 (41%), Positives = 160/281 (56%), Gaps = 20/281 (7%)
 Frame = -2

Query: 1145 RRFELLSHLFGWLVENLKTLNPVGSKESAETK-------GLPRATXXXXXXXXXXXXLPG 987
            R+ + LS LF WLV  LK L P+  K+SA           L  A               G
Sbjct: 339  RQIDRLSSLFAWLVGQLKGLKPLRCKQSAAESLASSIGMNLSNAILMEVLRKCLLVFSYG 398

Query: 986  NKQLMASAVTLAQLSGASSLIQKLKKLALLEL--PSVSEPNSISAYDDTLLSQQERSIRQ 813
            NKQLM SA+ LAQL G SS++ KLKKL+LL L  P V++  S     ++ L+QQ++SI Q
Sbjct: 399  NKQLMGSALHLAQLMGDSSVMDKLKKLSLLALSDPEVTQEKSPPLSLNSFLTQQDQSIHQ 458

Query: 812  AEGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTIGFSGRLPTLD 633
            A  KL+ +K  R + K    T  +  G+  RW VAKSW PCPIGMLPR +G SG LP LD
Sbjct: 459  ATKKLDFVKLCRTKSKVAKRTDGDV-GSSGRWIVAKSWNPCPIGMLPRDLGSSGCLPVLD 517

Query: 632  CVDNRREVTTASDIKEV---------SKQSHRKREAEISTQELDVTPVKKVEMEADCSMQ 480
            C D+ ++   AS+ K++          +++  KREA+     LD   VKK+   AD S +
Sbjct: 518  CDDDGKKSVHASERKQIWELKQYYTSVERTGSKREADSDIYLLDKASVKKMRETAD-SFE 576

Query: 479  NRSDS--SAEDIEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            +  ++   ++D +G LMI+G WKKVGE+ELL I S V++L+
Sbjct: 577  SEGENVLLSKDDKGCLMINGVWKKVGEEELLAIMSDVRILV 617


>ref|XP_011036402.1| PREDICTED: uncharacterized protein LOC105133938 [Populus euphratica]
          Length = 634

 Score =  171 bits (434), Expect = 9e-40
 Identities = 116/294 (39%), Positives = 159/294 (54%), Gaps = 33/294 (11%)
 Frame = -2

Query: 1145 RRFELLSHLFGWLVENLKTLNPVGSKESAETK-------GLPRATXXXXXXXXXXXXLPG 987
            R+ + LS LF WLVE LK L P+  K+ A           L  A               G
Sbjct: 343  RQIDRLSSLFAWLVEQLKGLKPLRCKQPAAESLASSIGMNLSNAILMEVLRKCLLVSSYG 402

Query: 986  NKQLMASAVTLAQLSGASSLIQKLKKLALLEL--PSVSEPNSISAYDDTLLSQQERSIRQ 813
            NKQLM SA+ LA+L G SS+I KLKKL+LL L  P V++  S     ++ L+QQ++SI Q
Sbjct: 403  NKQLMGSALHLARLMGDSSVIDKLKKLSLLALSDPDVTQEKSPPLSLNSFLTQQDQSIHQ 462

Query: 812  AEGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTIGFSGRLPTLD 633
            A  KL+ +K  R + K    T  +  G+  RW VAKSW PCPIGMLPR +G SG LP LD
Sbjct: 463  ATKKLDFVKLCRTKSKVAKRTDGDV-GSSGRWVVAKSWNPCPIGMLPRDLGSSGCLPVLD 521

Query: 632  CVDNRREVTTASDIKEVSKQSH----------------------RKREAEISTQELDVTP 519
            C D+ ++   AS+ K++ +Q                         KREA      LD + 
Sbjct: 522  CDDDGKKPVHASERKQIWEQKQCSIREPGVDIPLSDYTGVERTGSKREAGSDIYLLDKSS 581

Query: 518  VKKVEMEADCSMQNRSDS--SAEDIEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            VKK+   AD S ++  ++   ++D +G LMI+G WKK+GE+ELL I S V++L+
Sbjct: 582  VKKMRETAD-SFESEGENLLLSKDDQGCLMINGVWKKIGEEELLAIMSDVRILV 634


>ref|XP_010656891.1| PREDICTED: pre-rRNA-processing protein las1 [Vitis vinifera]
          Length = 624

 Score =  170 bits (431), Expect = 2e-39
 Identities = 110/276 (39%), Positives = 153/276 (55%), Gaps = 10/276 (3%)
 Frame = -2

Query: 1160 DSVKMRRFELLSHLFGWLVENLKTLNPVGSKESAETKGLPRATXXXXXXXXXXXXLPGNK 981
            D V++ + ELLS LF WLVE+ K     G     +   +P+AT             P NK
Sbjct: 357  DDVEIGQIELLSSLFAWLVEHFK-----GFYSEIQ---IPKATLLELLRKCLLVSAPDNK 408

Query: 980  QLMASAVTLAQLSGASSLIQKLKKLALLELPS--VSEPNSISAYDDTLLSQQERSIRQAE 807
             +M SA+ LAQ++  + +I+KL +  LL L    V+E  +     + LL  QE +IRQA 
Sbjct: 409  LIMGSALLLAQMAENNIMIEKLSRFCLLGLSKSDVTEETAPPLSFEKLLVHQEDAIRQAA 468

Query: 806  GKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTIGFSGRLPTLDCV 627
             KLEL+KR R++      T     G  SRW VAKSW PCPIGMLPR +G SGRLP LD  
Sbjct: 469  EKLELVKRHRVKGSIVKTTTNGDDGNHSRWVVAKSWNPCPIGMLPRALGSSGRLPALDHN 528

Query: 626  DNRREVTTASDIKEVSKQSH--RKREAEISTQELDVTPVKKVEMEADCSMQNRSDSSAED 453
            DN++    +S+ +E+ +  H  RKREA    + LD + VKK+    +       DS  +D
Sbjct: 529  DNQKMPPESSERRELWELKHCSRKREACFDIEMLDNSSVKKMRETVEDQTIEGHDSDDKD 588

Query: 452  ------IEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
                  ++G L+I+G WKKV  +ELL I SAV++L+
Sbjct: 589  VTLCEGVKGQLLINGVWKKVEREELLAIKSAVRILV 624


>emb|CBI17902.3| unnamed protein product [Vitis vinifera]
          Length = 547

 Score =  170 bits (431), Expect = 2e-39
 Identities = 110/276 (39%), Positives = 153/276 (55%), Gaps = 10/276 (3%)
 Frame = -2

Query: 1160 DSVKMRRFELLSHLFGWLVENLKTLNPVGSKESAETKGLPRATXXXXXXXXXXXXLPGNK 981
            D V++ + ELLS LF WLVE+ K     G     +   +P+AT             P NK
Sbjct: 280  DDVEIGQIELLSSLFAWLVEHFK-----GFYSEIQ---IPKATLLELLRKCLLVSAPDNK 331

Query: 980  QLMASAVTLAQLSGASSLIQKLKKLALLELPS--VSEPNSISAYDDTLLSQQERSIRQAE 807
             +M SA+ LAQ++  + +I+KL +  LL L    V+E  +     + LL  QE +IRQA 
Sbjct: 332  LIMGSALLLAQMAENNIMIEKLSRFCLLGLSKSDVTEETAPPLSFEKLLVHQEDAIRQAA 391

Query: 806  GKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTIGFSGRLPTLDCV 627
             KLEL+KR R++      T     G  SRW VAKSW PCPIGMLPR +G SGRLP LD  
Sbjct: 392  EKLELVKRHRVKGSIVKTTTNGDDGNHSRWVVAKSWNPCPIGMLPRALGSSGRLPALDHN 451

Query: 626  DNRREVTTASDIKEVSKQSH--RKREAEISTQELDVTPVKKVEMEADCSMQNRSDSSAED 453
            DN++    +S+ +E+ +  H  RKREA    + LD + VKK+    +       DS  +D
Sbjct: 452  DNQKMPPESSERRELWELKHCSRKREACFDIEMLDNSSVKKMRETVEDQTIEGHDSDDKD 511

Query: 452  ------IEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
                  ++G L+I+G WKKV  +ELL I SAV++L+
Sbjct: 512  VTLCEGVKGQLLINGVWKKVEREELLAIKSAVRILV 547


>ref|XP_008227626.1| PREDICTED: uncharacterized protein LOC103327116 [Prunus mume]
            gi|645242630|ref|XP_008227627.1| PREDICTED:
            uncharacterized protein LOC103327116 [Prunus mume]
          Length = 640

 Score =  165 bits (417), Expect = 9e-38
 Identities = 114/292 (39%), Positives = 154/292 (52%), Gaps = 12/292 (4%)
 Frame = -2

Query: 1208 TESCCLSASGDYQSPLDSVKMRRFELLSHLFGWLVENLKTLNPVGSKESAET-------K 1050
            T+   L  +G  ++  D  ++ + E LS LF WLV N + L P   K+SA         K
Sbjct: 331  TQEAVLFETGGVRTSSDRAEILQVEHLSSLFKWLVGNFEGLKPHLEKDSAPEIKVSSGEK 390

Query: 1049 GLPRATXXXXXXXXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALLELPS--VSE 876
             + +A                N QLM SAV LAQL+G + L+ KL KL+ L   +  V+E
Sbjct: 391  TISKAILMELLRKCLVLSASANNQLMDSAVHLAQLTGDNYLLGKLNKLSSLVSSNLDVTE 450

Query: 875  PNSISAYDDTLLSQQERSIRQAEGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWT 696
             +    +   +   ++ SI +A  KLE+IK+++M+ K    T  +A G  SRW +AKSW 
Sbjct: 451  EDDSLKHSTNVFKNEDESISEAAKKLEMIKQRKMKSKVVKRTDGDA-GNTSRWVLAKSWN 509

Query: 695  PCPIGMLPRTIGFSGRLPTLDCVDNRREVTTASDIKE--VSKQSHRKREAEISTQELDVT 522
            PCPIGMLP+ +G SG LP LD      +V+     KE     Q   KREA    Q LD  
Sbjct: 510  PCPIGMLPQAVGSSGCLPVLDFNTAPEKVSQLLGRKENWELNQCSGKREASSDIQLLDNR 569

Query: 521  PVKKV-EMEADCSMQNRSDSSAEDIEGHLMIDGAWKKVGEQELLGIASAVKL 369
             VKK+ E E  C   +    S ED+ GHLMI G WKKVGE+ELL I SAV++
Sbjct: 570  CVKKLRETEDGCVSDSEHVLSTEDVSGHLMIGGVWKKVGEEELLAIKSAVRI 621


>ref|XP_002298459.2| las1-like family protein [Populus trichocarpa]
            gi|550348351|gb|EEE83264.2| las1-like family protein
            [Populus trichocarpa]
          Length = 626

 Score =  159 bits (403), Expect = 4e-36
 Identities = 112/287 (39%), Positives = 153/287 (53%), Gaps = 27/287 (9%)
 Frame = -2

Query: 1142 RFELLSHLFGWLVENLKTLNPVGSKESAETK-------GLPRATXXXXXXXXXXXXLPGN 984
            + E LS LF WLV  LK L P+  KE+A  +        L                  GN
Sbjct: 342  QIERLSSLFAWLVGQLKGLKPLRCKETAAERKASSIGMNLSNTILMEVLRKCLLVSSNGN 401

Query: 983  KQLMASAVTLAQLSGASSLIQKLKKLALLEL--PSVSEPNSISAYDDTLLSQQERSIRQA 810
            KQLM SA+ LAQL G +S++ KLKKL+ L L  P V++  S     + LL QQ+ SI QA
Sbjct: 402  KQLMDSALHLAQLMGDTSVMGKLKKLSSLVLSDPDVTQEKSSLPSLNNLLIQQDESIHQA 461

Query: 809  EGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTIGFSGRLPTLDC 630
              KLE +K  R + K    T  E  G+   W+VAKSW PCPIGMLPR +G SG LP LDC
Sbjct: 462  TKKLEFVKFCRTKSKAVKRTDGEV-GSSGGWAVAKSWNPCPIGMLPRDLGSSGHLPVLDC 520

Query: 629  VDNRREVTTASDIKEV----------------SKQSHRKREAEISTQELDVTPVKKVEME 498
             D+ ++   +S+ K+                  +++  KREA      LD +  KK+   
Sbjct: 521  ADDGKKPVHSSEWKQSWELKQGSSGDIRFSYGVERTSSKREAGCDIYLLDKSSAKKMRET 580

Query: 497  ADCSMQNRSDS--SAEDIEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            AD S ++  ++   + D +G LMI+G WKK+GE+ELL I S V++L+
Sbjct: 581  AD-SFESDCENVLLSGDDKGCLMINGVWKKIGEEELLTIMSDVRILV 626


>gb|ABK93614.1| unknown [Populus trichocarpa]
          Length = 311

 Score =  159 bits (403), Expect = 4e-36
 Identities = 112/287 (39%), Positives = 153/287 (53%), Gaps = 27/287 (9%)
 Frame = -2

Query: 1142 RFELLSHLFGWLVENLKTLNPVGSKESAETK-------GLPRATXXXXXXXXXXXXLPGN 984
            + E LS LF WLV  LK L P+  KE+A  +        L                  GN
Sbjct: 27   QIERLSSLFAWLVGQLKGLKPLRCKETAAERKASSIGMNLSNTILMEVLRKCLLVSSNGN 86

Query: 983  KQLMASAVTLAQLSGASSLIQKLKKLALLEL--PSVSEPNSISAYDDTLLSQQERSIRQA 810
            KQLM SA+ LAQL G +S++ KLKKL+ L L  P V++  S     + LL QQ+ SI QA
Sbjct: 87   KQLMDSALHLAQLMGDTSVMGKLKKLSSLVLSDPDVTQEKSSLPSLNNLLIQQDESIHQA 146

Query: 809  EGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTIGFSGRLPTLDC 630
              KLE +K  R + K    T  E  G+   W+VAKSW PCPIGMLPR +G SG LP LDC
Sbjct: 147  TKKLEFVKFCRTKSKAVKRTDGEV-GSSGGWAVAKSWNPCPIGMLPRDLGSSGHLPVLDC 205

Query: 629  VDNRREVTTASDIKEV----------------SKQSHRKREAEISTQELDVTPVKKVEME 498
             D+ ++   +S+ K+                  +++  KREA      LD +  KK+   
Sbjct: 206  ADDGKKPVHSSEWKQSWELKQGSSGDIRFSYGVERTSSKREAGCDIYLLDKSSAKKMRET 265

Query: 497  ADCSMQNRSDS--SAEDIEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            AD S ++  ++   + D +G LMI+G WKK+GE+ELL I S V++L+
Sbjct: 266  AD-SFESDCENVLLSGDDKGCLMINGVWKKIGEEELLTIMSDVRILV 311


>ref|XP_007198899.1| hypothetical protein PRUPE_ppa003025mg [Prunus persica]
            gi|462394194|gb|EMJ00098.1| hypothetical protein
            PRUPE_ppa003025mg [Prunus persica]
          Length = 611

 Score =  159 bits (401), Expect = 6e-36
 Identities = 107/270 (39%), Positives = 146/270 (54%), Gaps = 12/270 (4%)
 Frame = -2

Query: 1136 ELLSHLFGWLVENLKTLNPVGSKESAET-------KGLPRATXXXXXXXXXXXXLPGNKQ 978
            E LS LF WLV+  + L P   K+S          K + +A                N Q
Sbjct: 343  EHLSSLFKWLVKKFEGLKPHFEKDSVAEIKVSSGEKTISKAILMELLRKCLVLSASANNQ 402

Query: 977  LMASAVTLAQLSGASSLIQKLKKLALLELPS--VSEPNSISAYDDTLLSQQERSIRQAEG 804
            LM SA+ LAQL+G + L+ KL KL+ L   +  V+E      + + +  +++ S+ +A  
Sbjct: 403  LMDSAIDLAQLTGDNYLLGKLNKLSSLVSSNSDVTEEGDSLKHSNNVFKKKDESMSEAAK 462

Query: 803  KLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTIGFSGRLPTLDCVD 624
            KLE+IK  ++++K    T  +A  T +RW +AKSW PCPIGMLPR +GFSG LP LD   
Sbjct: 463  KLEMIKCHKIKKKVVKRTDADARNT-NRWVLAKSWNPCPIGMLPRAVGFSGCLPVLDFNI 521

Query: 623  NRREVTTASDIKE--VSKQSHRKREAEISTQELDVTPVKKV-EMEADCSMQNRSDSSAED 453
               +V+     KE     Q   KREA      LD   VKK+ E E  C   +   SS ED
Sbjct: 522  VPEKVSQLIGRKENWELNQCSGKREASSDLHLLDNQCVKKLRETEDGCVSDSEHVSSIED 581

Query: 452  IEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            + GHLMIDG WK+VGE+ELL I SA+++LI
Sbjct: 582  VSGHLMIDGVWKRVGEEELLAIKSALRILI 611


>ref|XP_010097924.1| hypothetical protein L484_009358 [Morus notabilis]
            gi|587884384|gb|EXB73280.1| hypothetical protein
            L484_009358 [Morus notabilis]
          Length = 683

 Score =  157 bits (397), Expect = 2e-35
 Identities = 111/295 (37%), Positives = 158/295 (53%), Gaps = 12/295 (4%)
 Frame = -2

Query: 1211 GTESCCLSASGDYQSPLDSVKMRRFELLSHLFGWLVENLKTLNPVGSKESA-ETKG---- 1047
            GTE   LS +G          +RR E L+ LF WLV+ LK L P   K+SA +TK     
Sbjct: 399  GTECLTLSDNGR--------DVRRVEYLASLFAWLVQILKELKPRRHKDSAAKTKSSSAE 450

Query: 1046 --LPRATXXXXXXXXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSVSE- 876
              + +A              PGNK LM SA+ + QL G + L++KL KL+ L + S SE 
Sbjct: 451  STISKALLMQLLRKCLALSAPGNKHLMDSALYITQLIGCNHLMKKLNKLSSL-VSSNSEI 509

Query: 875  -PNSISAYDDTLLSQQERSIRQAEGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSW 699
              +  S ++   L Q++ SI QA  KLELIK  RM+RK  N    +  G   RW+V+ SW
Sbjct: 510  IEDKSSLFNSKSLIQRDESISQAAKKLELIKHLRMKRKVANAA-DDDMGNSRRWAVSTSW 568

Query: 698  TPCPIGMLPRTIGFSGRLPTLDCVDNRREVTTASDIKEVSKQSHRKREAEISTQELDVTP 519
             PCPIGMLP ++G SG LP LDC  ++++ +     +E  + +        +   LD + 
Sbjct: 569  IPCPIGMLPNSVGSSGCLPVLDCKGDQKKNSETQQARENLEFNCYSEAIGPTPALLDGSG 628

Query: 518  VKK--VEMEADCSMQNRSD-SSAEDIEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            VKK  + +E D       D S  E ++G LMI G WK VGE+++L I S+V++L+
Sbjct: 629  VKKRRLTVEGDFESNRGEDVSILEGLDGQLMIGGVWKTVGEEDILDIKSSVRILV 683


>ref|XP_011464875.1| PREDICTED: protein LAS1 isoform X2 [Fragaria vesca subsp. vesca]
          Length = 623

 Score =  157 bits (397), Expect = 2e-35
 Identities = 108/284 (38%), Positives = 152/284 (53%), Gaps = 21/284 (7%)
 Frame = -2

Query: 1151 KMRRFELLSHLFGWLVENLKTLNPVGS------KESAETKGLPRATXXXXXXXXXXXXLP 990
            K+ + E LS LF WLV + K L P         K S+  K + +A               
Sbjct: 341  KIYQVEHLSSLFTWLVRSFKGLKPPEEDSADEVKVSSAEKTISKAVLMDVLRKCLLVSAS 400

Query: 989  GNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSV--SEPNSISAYDDTLLSQQERSIR 816
             N  LM SA+ LA+L+G   L+QKL KL+ L   ++  ++ N+  A  +++  QQE+SI 
Sbjct: 401  ANNHLMKSALHLAKLTGDEYLVQKLNKLSFLVSSNLDAAKENTCLASSNSIFVQQEKSIS 460

Query: 815  QAEGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTIGFSGRLPTL 636
            QA  KLELIKR +M+R+    T     G+  RW +AKSWT CPIGMLP  +G SG LP L
Sbjct: 461  QAAKKLELIKRHQMKREAVKGTRDADVGSSKRWVMAKSWTRCPIGMLPCAVGHSGSLPIL 520

Query: 635  D---CVDNRREVTTASDIKEVSKQSHRKREAEISTQELDVTPVKKV----------EMEA 495
            D     +   EV   ++  E++  + +KR A    Q  D    KK+          +ME 
Sbjct: 521  DYNGASEQDPEVPGENEKWELNSCTGKKR-ANSDMQLFDNPCAKKMRGTVVDDYVSDMED 579

Query: 494  DCSMQNRSDSSAEDIEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
              S +  +  S+ED+ GHLMI G WKKVGE+E+L I SAV++L+
Sbjct: 580  VLSSEEVNAMSSEDVCGHLMIGGVWKKVGEEEILAIKSAVRILV 623


>ref|XP_004299975.1| PREDICTED: uncharacterized protein LOC101296866 isoform X1 [Fragaria
            vesca subsp. vesca]
          Length = 651

 Score =  157 bits (397), Expect = 2e-35
 Identities = 108/284 (38%), Positives = 152/284 (53%), Gaps = 21/284 (7%)
 Frame = -2

Query: 1151 KMRRFELLSHLFGWLVENLKTLNPVGS------KESAETKGLPRATXXXXXXXXXXXXLP 990
            K+ + E LS LF WLV + K L P         K S+  K + +A               
Sbjct: 369  KIYQVEHLSSLFTWLVRSFKGLKPPEEDSADEVKVSSAEKTISKAVLMDVLRKCLLVSAS 428

Query: 989  GNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSV--SEPNSISAYDDTLLSQQERSIR 816
             N  LM SA+ LA+L+G   L+QKL KL+ L   ++  ++ N+  A  +++  QQE+SI 
Sbjct: 429  ANNHLMKSALHLAKLTGDEYLVQKLNKLSFLVSSNLDAAKENTCLASSNSIFVQQEKSIS 488

Query: 815  QAEGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRTIGFSGRLPTL 636
            QA  KLELIKR +M+R+    T     G+  RW +AKSWT CPIGMLP  +G SG LP L
Sbjct: 489  QAAKKLELIKRHQMKREAVKGTRDADVGSSKRWVMAKSWTRCPIGMLPCAVGHSGSLPIL 548

Query: 635  D---CVDNRREVTTASDIKEVSKQSHRKREAEISTQELDVTPVKKV----------EMEA 495
            D     +   EV   ++  E++  + +KR A    Q  D    KK+          +ME 
Sbjct: 549  DYNGASEQDPEVPGENEKWELNSCTGKKR-ANSDMQLFDNPCAKKMRGTVVDDYVSDMED 607

Query: 494  DCSMQNRSDSSAEDIEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
              S +  +  S+ED+ GHLMI G WKKVGE+E+L I SAV++L+
Sbjct: 608  VLSSEEVNAMSSEDVCGHLMIGGVWKKVGEEEILAIKSAVRILV 651


>ref|XP_007214532.1| hypothetical protein PRUPE_ppa003033mg [Prunus persica]
            gi|462410397|gb|EMJ15731.1| hypothetical protein
            PRUPE_ppa003033mg [Prunus persica]
          Length = 610

 Score =  157 bits (397), Expect = 2e-35
 Identities = 115/291 (39%), Positives = 150/291 (51%), Gaps = 14/291 (4%)
 Frame = -2

Query: 1208 TESCCLSASGDYQSPLDS-VKMRRFELLSHLFGWLVENLKTLNPVGSKESAET------- 1053
            T+   L  +G  ++  D   ++ + E LS LF WLV N + L P   K SA         
Sbjct: 319  TQEAVLFETGGVRTSSDHRAEILQVEHLSSLFKWLVGNFEGLKPHLEKYSAPEIKVSSGE 378

Query: 1052 KGLPRATXXXXXXXXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALL---ELPSV 882
            K + +A                N QLM SAV LAQL+G + L+ KL KL+ L    L   
Sbjct: 379  KTISKAILMELLRKCLVLSASANNQLMDSAVHLAQLTGDNYLLGKLNKLSSLVSSNLDVT 438

Query: 881  SEPNSISAYDDTLLSQQERSIRQAEGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKS 702
             E NS+  +   +   ++ SI +A  KLE+IK+++M+ K    T  +A G  +RW +AKS
Sbjct: 439  EEDNSLK-HSTNVFKNEDESISEASKKLEMIKQRKMKSKVVKGTDGDA-GNTNRWVLAKS 496

Query: 701  WTPCPIGMLPRTIGFSGRLPTLDCVDNRREVTTASDIKE--VSKQSHRKREAEISTQELD 528
            W PCPIGMLPR +G SG LP LD      +V+     KE     Q   KREA    Q LD
Sbjct: 497  WNPCPIGMLPRAVGSSGCLPVLDFDTVPEKVSQLLGRKENWELNQCSGKREASSDIQLLD 556

Query: 527  VTPVKKV-EMEADCSMQNRSDSSAEDIEGHLMIDGAWKKVGEQELLGIASA 378
               VKK+ E E  C   +    S ED+ GHLMI G WKKVGE+ELL I SA
Sbjct: 557  NRCVKKLRETEDGCVSDSEHVLSTEDVSGHLMIGGVWKKVGEEELLAIKSA 607


>ref|XP_008449642.1| PREDICTED: pre-rRNA-processing protein las1 [Cucumis melo]
          Length = 607

 Score =  156 bits (395), Expect = 3e-35
 Identities = 108/285 (37%), Positives = 145/285 (50%), Gaps = 8/285 (2%)
 Frame = -2

Query: 1193 LSASGDYQSPLD-SVKMRRFELLSHLFGWLVENLKTLNPVGSKESAETKGLPRATXXXXX 1017
            L     Y  P D  V+  +   LS LF WLV+ L  +      E    K +P+       
Sbjct: 325  LEYESQYFMPSDHKVESCQVVQLSSLFAWLVDKLNGIKLKRKGEVFSGKHIPKGILMELL 384

Query: 1016 XXXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSVSEP---NSISAYDDT 846
                    P NK+L+ SA  LAQL    SL++KL KL+ + +P    P   N      + 
Sbjct: 385  RKCLLVSAPWNKRLLDSATDLAQLLDDDSLVKKLNKLSFVNVPISPIPDGENPNQTASEN 444

Query: 845  LLSQQERSIRQAEGKLELIKRQRMQRKHGNPTLTEAWGTKSRWSVAKSWTPCPIGMLPRT 666
              +Q E S+R+A  KLE+IK ++ +    +  +    G  SRW+V  SWTPCPIGMLPR+
Sbjct: 445  SFNQAE-SLREASRKLEMIKLRKRKNNLASNPVDGNVGCSSRWTVVSSWTPCPIGMLPRS 503

Query: 665  IGFSGRLPTLDCVDNRR---EVTTASDIKEVSKQSHRKREAEISTQELDVTPVKKVEMEA 495
            IG SGRL  LD         E   + D + ++  SH KREA    Q LD + VKK++   
Sbjct: 504  IGCSGRLSVLDLNPENEPASEEPRSKDNRALNNSSH-KREASSDIQHLDSSIVKKMKETN 562

Query: 494  DCSMQNRSD-SSAEDIEGHLMIDGAWKKVGEQELLGIASAVKLLI 363
            D S     D  S E IEG L+I G WKKVGE+EL  I S+V++LI
Sbjct: 563  DKSQFETEDVESPEGIEGRLLIGGIWKKVGEEELSAIQSSVRILI 607


>ref|XP_009353741.1| PREDICTED: uncharacterized protein LOC103944976 isoform X2 [Pyrus x
            bretschneideri]
          Length = 647

 Score =  156 bits (394), Expect = 4e-35
 Identities = 115/301 (38%), Positives = 154/301 (51%), Gaps = 17/301 (5%)
 Frame = -2

Query: 1214 EGTESCCLSASGDYQSPLDSVKMRRFELLSHLFGWLVENLKTLNPVGSKESAET------ 1053
            E  E+      G   S     ++ + E LS LF WLV+N + L P    +SA        
Sbjct: 355  ETRETVIYETGGISTSSDRRAEVLQVEHLSSLFAWLVQNFEGLKPRHEMDSAAEIKVSSA 414

Query: 1052 -KGLPRATXXXXXXXXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSVSE 876
             K + +A                NK+L  SA  LAQL+G S L+ KL KL+     S   
Sbjct: 415  EKTISKAILMELLRKCLIVSASANKKLTDSAAHLAQLAGDSCLVGKLHKLS-----SFVS 469

Query: 875  PNSISAYDDTL-------LSQQERSIRQAEGKLELIKRQRMQRKHGNPTLTEAWGTKSRW 717
             NS    +DT+         +QE SI QA  KLELIKR+ ++ K       +  G   RW
Sbjct: 470  SNSDGTEEDTVHINSNNQFKKQEESISQAAEKLELIKRRIVKSKVAKRIDADV-GDSDRW 528

Query: 716  SVAKSWTPCPIGMLPRTIGFSGRLPTLD--CVDNRREVTTASDIKEVSKQSHRKREAEIS 543
             +AKSW PCPIGMLPR +G SGRLP LD    +   E+    +  E+++ S ++  +++ 
Sbjct: 529  VLAKSWNPCPIGMLPRAVGSSGRLPVLDVNVPEKVPELLGRKEKWELNQCSVKRPSSDM- 587

Query: 542  TQELDVTPVKKVEMEADCSMQNRSD-SSAEDIEGHLMIDGAWKKVGEQELLGIASAVKLL 366
             Q LD   VKK+    D S     D SS ED++GHLMI G WKKVGE+ELL I SAV++L
Sbjct: 588  -QLLDNPCVKKMRAAVDGSASEGEDVSSWEDVDGHLMIGGVWKKVGEEELLAIKSAVRIL 646

Query: 365  I 363
            I
Sbjct: 647  I 647


>ref|XP_009353740.1| PREDICTED: uncharacterized protein LOC103944976 isoform X1 [Pyrus x
            bretschneideri]
          Length = 659

 Score =  156 bits (394), Expect = 4e-35
 Identities = 115/301 (38%), Positives = 154/301 (51%), Gaps = 17/301 (5%)
 Frame = -2

Query: 1214 EGTESCCLSASGDYQSPLDSVKMRRFELLSHLFGWLVENLKTLNPVGSKESAET------ 1053
            E  E+      G   S     ++ + E LS LF WLV+N + L P    +SA        
Sbjct: 367  ETRETVIYETGGISTSSDRRAEVLQVEHLSSLFAWLVQNFEGLKPRHEMDSAAEIKVSSA 426

Query: 1052 -KGLPRATXXXXXXXXXXXXLPGNKQLMASAVTLAQLSGASSLIQKLKKLALLELPSVSE 876
             K + +A                NK+L  SA  LAQL+G S L+ KL KL+     S   
Sbjct: 427  EKTISKAILMELLRKCLIVSASANKKLTDSAAHLAQLAGDSCLVGKLHKLS-----SFVS 481

Query: 875  PNSISAYDDTL-------LSQQERSIRQAEGKLELIKRQRMQRKHGNPTLTEAWGTKSRW 717
             NS    +DT+         +QE SI QA  KLELIKR+ ++ K       +  G   RW
Sbjct: 482  SNSDGTEEDTVHINSNNQFKKQEESISQAAEKLELIKRRIVKSKVAKRIDADV-GDSDRW 540

Query: 716  SVAKSWTPCPIGMLPRTIGFSGRLPTLD--CVDNRREVTTASDIKEVSKQSHRKREAEIS 543
             +AKSW PCPIGMLPR +G SGRLP LD    +   E+    +  E+++ S ++  +++ 
Sbjct: 541  VLAKSWNPCPIGMLPRAVGSSGRLPVLDVNVPEKVPELLGRKEKWELNQCSVKRPSSDM- 599

Query: 542  TQELDVTPVKKVEMEADCSMQNRSD-SSAEDIEGHLMIDGAWKKVGEQELLGIASAVKLL 366
             Q LD   VKK+    D S     D SS ED++GHLMI G WKKVGE+ELL I SAV++L
Sbjct: 600  -QLLDNPCVKKMRAAVDGSASEGEDVSSWEDVDGHLMIGGVWKKVGEEELLAIKSAVRIL 658

Query: 365  I 363
            I
Sbjct: 659  I 659