BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Perilla23_contig00010644
         (1232 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011082319.1| PREDICTED: diphthamide biosynthesis protein ...   642   0.0  
ref|XP_012854190.1| PREDICTED: diphthamide biosynthesis protein ...   640   0.0  
gb|EPS72161.1| hypothetical protein M569_02597 [Genlisea aurea]       561   e-157
emb|CDP11710.1| unnamed protein product [Coffea canephora]            519   e-144
ref|XP_006365966.1| PREDICTED: diphthamide biosynthesis protein ...   518   e-144
ref|XP_004241760.1| PREDICTED: diphthamide biosynthesis protein ...   515   e-143
ref|XP_009611471.1| PREDICTED: diphthamide biosynthesis protein ...   507   e-141
ref|XP_009759721.1| PREDICTED: diphthamide biosynthesis protein ...   506   e-140
ref|XP_007041598.1| Diphthamide synthesis DPH2 family protein [T...   495   e-137
ref|XP_012480536.1| PREDICTED: diphthamide biosynthesis protein ...   490   e-136
gb|KHF97888.1| Diphthamide biosynthesis 1 [Gossypium arboreum]        490   e-136
ref|XP_011010116.1| PREDICTED: diphthamide biosynthesis protein ...   489   e-135
gb|AIU49158.1| diphthamide synthesis DPH2 family protein, partia...   486   e-134
ref|XP_006280451.1| hypothetical protein CARUB_v10026383mg [Caps...   481   e-133
dbj|BAB10164.1| unnamed protein product [Arabidopsis thaliana]        474   e-131
ref|NP_201009.1| diphthamide synthesis DPH2 family protein [Arab...   474   e-131
ref|XP_002864766.1| diphthamide synthesis DPH2 family protein [A...   473   e-130
ref|XP_010458072.1| PREDICTED: diphthamide biosynthesis protein ...   473   e-130
ref|XP_002325444.1| diphthamide synthesis DPH2 family protein [P...   473   e-130
ref|XP_013630682.1| PREDICTED: diphthamide biosynthesis protein ...   471   e-130

>ref|XP_011082319.1| PREDICTED: diphthamide biosynthesis protein 1 [Sesamum indicum]
            gi|747070945|ref|XP_011082320.1| PREDICTED: diphthamide
            biosynthesis protein 1 [Sesamum indicum]
          Length = 452

 Score =  642 bits (1655), Expect = 0.0
 Identities = 312/359 (86%), Positives = 330/359 (91%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            L TFA VTHCFILGDVTYGACCVDDLSARALD HLLVHFGHSCLVPIDSTTIPVLYIFVE
Sbjct: 93   LKTFAGVTHCFILGDVTYGACCVDDLSARALDAHLLVHFGHSCLVPIDSTTIPVLYIFVE 152

Query: 1051 IAINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSA 872
            IAINTRKLL ELN+NF+PDK N+F+MAGTIQFSNAIRAVKPELE LGFRVLIPQSKPLSA
Sbjct: 153  IAINTRKLLDELNYNFSPDKTNSFIMAGTIQFSNAIRAVKPELENLGFRVLIPQSKPLSA 212

Query: 871  GEVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY 692
            GEVLGCTAPSVKLQ CAGKD+VIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY
Sbjct: 213  GEVLGCTAPSVKLQVCAGKDDVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY 272

Query: 691  DHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISEL 512
            DHEGMKGDRR+AIERA GAK+WGIVLGTLGRQGNP+ L RLEGKMRD+GLDYMVVLISEL
Sbjct: 273  DHEGMKGDRRRAIERAIGAKSWGIVLGTLGRQGNPMVLERLEGKMRDRGLDYMVVLISEL 332

Query: 511  SPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLECNG 332
            SPKKIELFGDAVDAW+QIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKL  NG
Sbjct: 333  SPKKIELFGDAVDAWVQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLRSNG 392

Query: 331  RLDGSKKEGCCGNENVQKGVDYPMDYYAQDGGEWNSCYSKKPARDWGRKIQSCNSR*GQ 155
              + ++   CC NENVQ+ VDYPMDYYAQDGGEWNSCY KK AR  G+  Q C++  G+
Sbjct: 393  SSECTRSGDCCRNENVQERVDYPMDYYAQDGGEWNSCYLKKSARVRGKNNQVCSNSSGK 451


>ref|XP_012854190.1| PREDICTED: diphthamide biosynthesis protein 1 [Erythranthe guttatus]
            gi|604303962|gb|EYU23312.1| hypothetical protein
            MIMGU_mgv1a026412mg [Erythranthe guttata]
          Length = 459

 Score =  640 bits (1651), Expect = 0.0
 Identities = 312/358 (87%), Positives = 330/358 (92%), Gaps = 3/358 (0%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            LTTFAAVTHCF+LGDVTYGACCVDDLSARALD HLLVHFGHSCLVPIDSTTIPVLYIFVE
Sbjct: 97   LTTFAAVTHCFVLGDVTYGACCVDDLSARALDAHLLVHFGHSCLVPIDSTTIPVLYIFVE 156

Query: 1051 IAINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSA 872
            IAINTRKLLHELNHNFTPD+IN+FVMAGTIQFSNAIRAVKPELE LGFRVLIPQSKPLSA
Sbjct: 157  IAINTRKLLHELNHNFTPDQINSFVMAGTIQFSNAIRAVKPELENLGFRVLIPQSKPLSA 216

Query: 871  GEVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY 692
            GEVLGCTAPSVKLQ C GKD+VIIFVADGRFHLEAFMIANPEIRAFR+DPYLGKLFLEEY
Sbjct: 217  GEVLGCTAPSVKLQTCPGKDDVIIFVADGRFHLEAFMIANPEIRAFRFDPYLGKLFLEEY 276

Query: 691  DHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISEL 512
            DHEGMKGDRRKAIERA GAKTWGIVLGTLGRQGN + + RLE KM+DKGLDYMVVLISEL
Sbjct: 277  DHEGMKGDRRKAIERAMGAKTWGIVLGTLGRQGNTMIMKRLEEKMKDKGLDYMVVLISEL 336

Query: 511  SPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLE-CN 335
            SPKKIELFGDAVDAW+QIACPRLSIDWGDAFKKPLLT+FEAEIALGDL GWW+RK   CN
Sbjct: 337  SPKKIELFGDAVDAWVQIACPRLSIDWGDAFKKPLLTAFEAEIALGDLDGWWQRKSRCCN 396

Query: 334  GRLDGSKKEGCCGNEN--VQKGVDYPMDYYAQDGGEWNSCYSKKPARDWGRKIQSCNS 167
            G  + ++ E CCGN N  V++GVDYPMDYYAQDGG WNSCYSKKPAR   +  Q CN+
Sbjct: 397  GGSESTRNEECCGNGNGDVREGVDYPMDYYAQDGGGWNSCYSKKPARVSRKNNQCCNN 454


>gb|EPS72161.1| hypothetical protein M569_02597 [Genlisea aurea]
          Length = 440

 Score =  561 bits (1446), Expect = e-157
 Identities = 270/353 (76%), Positives = 303/353 (85%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            LT FA VTHCF+LGDVTYGACCVDDLS RAL VHLLVHFGHSCLVPI +TT+PVLYIFVE
Sbjct: 88   LTAFAGVTHCFVLGDVTYGACCVDDLSCRALAVHLLVHFGHSCLVPIGNTTVPVLYIFVE 147

Query: 1051 IAINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSA 872
            I INTR LL EL++NF P+K++NF+MAGTIQF NAIRAVKPELE LGFRVL+PQSKPLSA
Sbjct: 148  IGINTRNLLQELDYNFNPEKVSNFIMAGTIQFCNAIRAVKPELENLGFRVLMPQSKPLSA 207

Query: 871  GEVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY 692
            GEVLGCTAP+VKLQDC GK+++IIFV+DGRFHLEAFMIANP ++AFRYDPYLGKLFLEEY
Sbjct: 208  GEVLGCTAPTVKLQDCPGKEDIIIFVSDGRFHLEAFMIANPGVKAFRYDPYLGKLFLEEY 267

Query: 691  DHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISEL 512
            D+EGMK DRRKAIERA GAK+WGIVLGTLGRQG+P+ + RLE KM++KGLDY+ VL+SEL
Sbjct: 268  DYEGMKQDRRKAIERAMGAKSWGIVLGTLGRQGSPMLVERLEKKMQEKGLDYITVLLSEL 327

Query: 511  SPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLECNG 332
            SPKKI+LFGD+VDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGD  GWW+ K     
Sbjct: 328  SPKKIDLFGDSVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDRPGWWDMK----- 382

Query: 331  RLDGSKKEGCCGNENVQKGVDYPMDYYAQDGGEWNSCYSKKPARDWGRKIQSC 173
                 K    C N N ++ VDYPMDYY+QDGGEWNS Y KK +     KI  C
Sbjct: 383  ---PMKSSNGCRNRNEEECVDYPMDYYSQDGGEWNSSYLKKKSGRASGKIGQC 432


>emb|CDP11710.1| unnamed protein product [Coffea canephora]
          Length = 472

 Score =  519 bits (1336), Expect = e-144
 Identities = 255/373 (68%), Positives = 301/373 (80%), Gaps = 21/373 (5%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            L TF +VTHCF+LGDVTYGACCVDDLSA AL   LL+HFGHSCLVPID T IP LYIFVE
Sbjct: 93   LQTFTSVTHCFVLGDVTYGACCVDDLSAAALSADLLIHFGHSCLVPIDFTAIPCLYIFVE 152

Query: 1051 IAINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSA 872
            I I++ KL +++  NF+  +  +F++AGTIQFS+AIRAVKPELEKLGFRVL+PQ+KPLSA
Sbjct: 153  IFIDSNKLFNQIKLNFSSSENFSFILAGTIQFSHAIRAVKPELEKLGFRVLVPQAKPLSA 212

Query: 871  GEVLGCTAPSVKLQ------DCAGKDN---VIIFVADGRFHLEAFMIANPEIRAFRYDPY 719
            GEVLGCTAPSV +       +  GK+    VIIFVADGRFHLEAFMIANP ++ FRYDPY
Sbjct: 213  GEVLGCTAPSVSINKEVTRVEREGKEENEEVIIFVADGRFHLEAFMIANPGVKTFRYDPY 272

Query: 718  LGKLFLEEYDHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLD 539
            LGKLFLEEYD++GMK +RR+AIERA+GAK WG+VLGTLGRQGNP  L RLEGKM +KGLD
Sbjct: 273  LGKLFLEEYDYDGMKEERRRAIERARGAKNWGLVLGTLGRQGNPRILERLEGKMMEKGLD 332

Query: 538  YMVVLISELSPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGW 359
            +MVVL+SELSP++I LF D+VDAW+QIACPRLSIDWGDAF KPLLT+FEAEIALGDL GW
Sbjct: 333  WMVVLMSELSPQRIALFEDSVDAWVQIACPRLSIDWGDAFGKPLLTTFEAEIALGDLPGW 392

Query: 358  WERK------LECNGRLDGSKKEGCCGNENVQKG------VDYPMDYYAQDGGEWNSCYS 215
            WERK      L C+   + S+ + CC + N  +       VDYPMDYYAQDGGEWNSCY+
Sbjct: 393  WERKSKAGVELSCSSGSECSENKSCCASNNGDRKEKNEVVVDYPMDYYAQDGGEWNSCYT 452

Query: 214  KKPARDWGRKIQS 176
            KKP R+  + +Q+
Sbjct: 453  KKPTRNLRKNLQA 465


>ref|XP_006365966.1| PREDICTED: diphthamide biosynthesis protein 1-like [Solanum
            tuberosum]
          Length = 481

 Score =  518 bits (1335), Expect = e-144
 Identities = 256/367 (69%), Positives = 299/367 (81%), Gaps = 13/367 (3%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            L+TF + THCFILGDVT+GACCVDDLSA AL   LL+HFGHSCLVPIDSTTIP LYIFVE
Sbjct: 106  LSTFTSATHCFILGDVTFGACCVDDLSAGALSADLLIHFGHSCLVPIDSTTIPCLYIFVE 165

Query: 1051 IAINTRKLLHELNHNF-TPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLS 875
            I+I+  +LL+EL  NF   D  +N +MAGTIQF++AIRAVKPELEK+GF +LIPQ+KPLS
Sbjct: 166  ISIDVHRLLNELKLNFYNSDTYDNIIMAGTIQFASAIRAVKPELEKMGFSILIPQAKPLS 225

Query: 874  AGEVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEE 695
            AGEVLGCTAPSVK +   G++ V++FVADGRFHLEAFMIANP I+ +RYDP++GKLF+EE
Sbjct: 226  AGEVLGCTAPSVKNRFSDGENVVLVFVADGRFHLEAFMIANPGIKTYRYDPFIGKLFVEE 285

Query: 694  YDHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISE 515
            YDH+GMK +R++AIE+A+G K WGIVLGTLGRQGNP  L+RLE KM +KG+ + VVL+SE
Sbjct: 286  YDHKGMKEERKRAIEKARGVKNWGIVLGTLGRQGNPRILDRLEKKMSEKGMTWTVVLMSE 345

Query: 514  LSPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLE-- 341
            +SP +I LF DAVDAWIQIACPRLSIDWGDAFKKPLLT FEAEIALGDL+GWWERK    
Sbjct: 346  ISPTRIALFEDAVDAWIQIACPRLSIDWGDAFKKPLLTPFEAEIALGDLSGWWERKTSVN 405

Query: 340  ----CNGRLDGSKKEGC--CGNE----NVQKGVDYPMDYYAQDGGEWNSCYSKKPARDWG 191
                C+  +  SK E C  C N+      +  VDYPMDYYAQDGGEWNSCYSKKPAR   
Sbjct: 406  SDICCDEDMKCSKNESCGACDNDGEKVKEESQVDYPMDYYAQDGGEWNSCYSKKPARLSH 465

Query: 190  RKIQSCN 170
            R  QSCN
Sbjct: 466  RNSQSCN 472


>ref|XP_004241760.1| PREDICTED: diphthamide biosynthesis protein 1 [Solanum lycopersicum]
            gi|723709409|ref|XP_010322707.1| PREDICTED: diphthamide
            biosynthesis protein 1 [Solanum lycopersicum]
            gi|723709412|ref|XP_010322708.1| PREDICTED: diphthamide
            biosynthesis protein 1 [Solanum lycopersicum]
          Length = 483

 Score =  515 bits (1326), Expect = e-143
 Identities = 255/367 (69%), Positives = 296/367 (80%), Gaps = 13/367 (3%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            L+TF + THCFILGDVT+GACCVDDLSA AL   LL+HFGHSCLVPIDSTTIP LYIFVE
Sbjct: 108  LSTFTSATHCFILGDVTFGACCVDDLSAGALSADLLIHFGHSCLVPIDSTTIPCLYIFVE 167

Query: 1051 IAINTRKLLHELNHNF-TPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLS 875
            I+I+  +LL+EL  NF   D  +N +MAGTIQF++AIRAVKPELEKLGF +LIPQ+KPLS
Sbjct: 168  ISIDVHRLLNELKLNFYNSDTYDNIIMAGTIQFASAIRAVKPELEKLGFSILIPQAKPLS 227

Query: 874  AGEVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEE 695
            AGEVLGCTAPSVK +   G++ V+IFVADGRFHLEAFMIANP I+ +RYDP++GKLF+EE
Sbjct: 228  AGEVLGCTAPSVKNRFSDGENVVLIFVADGRFHLEAFMIANPGIKTYRYDPFIGKLFVEE 287

Query: 694  YDHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISE 515
            YDH+GMK +R++AIE+A+GAK WGIVLGTLGRQGNP  L+RLE KM +KG+ + VVL+SE
Sbjct: 288  YDHKGMKEERKRAIEKARGAKNWGIVLGTLGRQGNPRILDRLEKKMSEKGMTWTVVLMSE 347

Query: 514  LSPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLECN 335
            +SP +I LF DAVDAWIQIACPRLSIDWGDAFKKPLLT FEAEIALGDL+GWWER    N
Sbjct: 348  ISPTRIALFEDAVDAWIQIACPRLSIDWGDAFKKPLLTPFEAEIALGDLSGWWERTTSKN 407

Query: 334  GRLDGSKKEGCCGNENV------------QKGVDYPMDYYAQDGGEWNSCYSKKPARDWG 191
              +   +   C  NE+             +  VDYPMDYYAQDGGEWNSCYSKKPAR   
Sbjct: 408  SDICRDEVMKCSKNESCGACDKSGEEVKEETQVDYPMDYYAQDGGEWNSCYSKKPARLSQ 467

Query: 190  RKIQSCN 170
            R  QSCN
Sbjct: 468  RNSQSCN 474


>ref|XP_009611471.1| PREDICTED: diphthamide biosynthesis protein 1 [Nicotiana
            tomentosiformis]
          Length = 480

 Score =  507 bits (1305), Expect = e-141
 Identities = 262/370 (70%), Positives = 297/370 (80%), Gaps = 16/370 (4%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            L+TF +  HCFILGDVTYGACCVDDLSA AL   LL+HFGHSCLVPIDSTTIP LYIFVE
Sbjct: 103  LSTFTSAAHCFILGDVTYGACCVDDLSAAALSADLLIHFGHSCLVPIDSTTIPCLYIFVE 162

Query: 1051 IAINTRKLLHELNHNFT-PDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLS 875
            I+I+  +LLHEL  NF      +N +MAGTIQF+ AIRAVKPELEK+GF+VLIPQ+KPLS
Sbjct: 163  ISIDLYRLLHELKLNFNNTSTYDNIIMAGTIQFAGAIRAVKPELEKIGFKVLIPQAKPLS 222

Query: 874  AGEVLGCTAPSV--KLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFL 701
            AGEVLGCTAPSV  K  DC  +  V++FVADGRFHLEAFMIANP I+ +RYDPY+GKLFL
Sbjct: 223  AGEVLGCTAPSVMNKFSDC--ESVVLVFVADGRFHLEAFMIANPGIKTYRYDPYVGKLFL 280

Query: 700  EEYDHEGMKGDRRKAIERA-KGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVL 524
            EEYDH+GMK +R++AIE+A + AK WGIVLGTLGRQGNP  L+RLE KM +KG+ + VVL
Sbjct: 281  EEYDHKGMKEERKRAIEKARREAKNWGIVLGTLGRQGNPRILDRLEKKMAEKGMTWTVVL 340

Query: 523  ISELSPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKL 344
            +SE+SP +I LF DAVDAWIQIACPRLSIDWGDAF KPLLT FEAEIALGDL+GWWERK 
Sbjct: 341  MSEISPTRIALFEDAVDAWIQIACPRLSIDWGDAFTKPLLTPFEAEIALGDLSGWWERKK 400

Query: 343  ECNG------RLDGSKKE--GCC--GNENVQKG--VDYPMDYYAQDGGEWNSCYSKKPAR 200
              N        L  SK E  G C  G E V++G  VDYPMDYYAQDGGEWNSCYSKKPAR
Sbjct: 401  AVNSDVCYDEALKCSKNESSGACDNGGEKVKEGVHVDYPMDYYAQDGGEWNSCYSKKPAR 460

Query: 199  DWGRKIQSCN 170
               R  Q+CN
Sbjct: 461  LSQRINQACN 470


>ref|XP_009759721.1| PREDICTED: diphthamide biosynthesis protein 1 [Nicotiana sylvestris]
          Length = 480

 Score =  506 bits (1304), Expect = e-140
 Identities = 261/370 (70%), Positives = 298/370 (80%), Gaps = 16/370 (4%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            ++TF +  HCFILGDVTYGACCVDDLSA AL   LL+HFGHSCLVPIDSTTIP LYIFVE
Sbjct: 103  ISTFTSAAHCFILGDVTYGACCVDDLSAAALSADLLIHFGHSCLVPIDSTTIPCLYIFVE 162

Query: 1051 IAINTRKLLHELNHNFT-PDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLS 875
            I+I+  +LLHEL  NF      +N +MAGTIQF+ AIRAVKPELEK+GF+VLIPQ+KPLS
Sbjct: 163  ISIDVYRLLHELKLNFNNTSTYDNIIMAGTIQFAGAIRAVKPELEKIGFKVLIPQAKPLS 222

Query: 874  AGEVLGCTAPSV--KLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFL 701
            AGEVLGCTAPSV  K  DC  +  V++FVADGRFHLEAFMIANP I+ +RYDPY+GKLFL
Sbjct: 223  AGEVLGCTAPSVMHKFSDC--ESVVLVFVADGRFHLEAFMIANPGIKTYRYDPYVGKLFL 280

Query: 700  EEYDHEGMKGDRRKAIERAK-GAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVL 524
            EEYDH+GMK +R++AIE+A+  AK WGIVLGTLGRQGNP  L+RLE KM +KG+ + VVL
Sbjct: 281  EEYDHKGMKEERKRAIEKARMEAKNWGIVLGTLGRQGNPRILDRLEKKMAEKGMTWTVVL 340

Query: 523  ISELSPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERK- 347
            +SE+SP +I LF  AVDAWIQIACPRLSIDWGDAF KPLLT FEAEIALGDL+GWWERK 
Sbjct: 341  MSEISPTRIALFEGAVDAWIQIACPRLSIDWGDAFTKPLLTPFEAEIALGDLSGWWERKK 400

Query: 346  -----LECNGRLDGSKKE--GCC--GNENVQKG--VDYPMDYYAQDGGEWNSCYSKKPAR 200
                 + C+  L  SK E  G C  G E V++G  VDYPMDYYAQDGGEWNSCYSKKPAR
Sbjct: 401  AVNSDVCCDEALKCSKNESSGACDNGGEKVKEGVLVDYPMDYYAQDGGEWNSCYSKKPAR 460

Query: 199  DWGRKIQSCN 170
               R  QSCN
Sbjct: 461  LSQRISQSCN 470


>ref|XP_007041598.1| Diphthamide synthesis DPH2 family protein [Theobroma cacao]
            gi|508705533|gb|EOX97429.1| Diphthamide synthesis DPH2
            family protein [Theobroma cacao]
          Length = 458

 Score =  495 bits (1274), Expect = e-137
 Identities = 249/352 (70%), Positives = 283/352 (80%), Gaps = 2/352 (0%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            LT+FA VTHCFILGDVTYGACC+DDLSA AL   LL+H+GHSCLVPIDST IP LY+FVE
Sbjct: 101  LTSFADVTHCFILGDVTYGACCIDDLSATALGADLLIHYGHSCLVPIDSTKIPCLYVFVE 160

Query: 1051 IAINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSA 872
            I IN   L++ + HN T  K  + V+AGTIQFS+AIR+ KPELEK GFRVLIPQSKPLSA
Sbjct: 161  IKINVDGLINTIEHNLTSSK-ESVVLAGTIQFSSAIRSAKPELEKRGFRVLIPQSKPLSA 219

Query: 871  GEVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY 692
            GEVLGCTAP +     A  + V +FVADGRFHLEAFMIANP I+AFRYDPYLGKLFLEEY
Sbjct: 220  GEVLGCTAPKIGTVANAN-ETVAVFVADGRFHLEAFMIANPGIKAFRYDPYLGKLFLEEY 278

Query: 691  DHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISEL 512
            DH+GM+  RR+AI++AK AK+WGIVLGTLGRQGNP  L RLEGKMR+KG DY ++L+SE+
Sbjct: 279  DHKGMRETRRRAIQKAKRAKSWGIVLGTLGRQGNPRVLERLEGKMREKGFDYTIILMSEI 338

Query: 511  SPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLECNG 332
            SP +I LF DAVDAWIQIACPRLSIDWGDAF KPLLT+FEAEIALG+++GWWER    NG
Sbjct: 339  SPTRIALFEDAVDAWIQIACPRLSIDWGDAFVKPLLTTFEAEIALGEISGWWERDGCGNG 398

Query: 331  RLDGSKKE--GCCGNENVQKGVDYPMDYYAQDGGEWNSCYSKKPARDWGRKI 182
               G  K   GC G E+ +   DYPMDYYAQDGGEWNS Y KK AR   R I
Sbjct: 399  LSCGESKSCCGCRGREDFRG--DYPMDYYAQDGGEWNSSYVKKLARPVRRNI 448


>ref|XP_012480536.1| PREDICTED: diphthamide biosynthesis protein 1 [Gossypium raimondii]
            gi|823161333|ref|XP_012480537.1| PREDICTED: diphthamide
            biosynthesis protein 1 [Gossypium raimondii]
            gi|763765493|gb|KJB32747.1| hypothetical protein
            B456_005G259400 [Gossypium raimondii]
          Length = 464

 Score =  490 bits (1262), Expect = e-136
 Identities = 244/354 (68%), Positives = 281/354 (79%), Gaps = 4/354 (1%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            LT+FA VTHCFILGDVTYGACCVDDLSA AL   LL+H+GHSCLVPID+T IP LY+FVE
Sbjct: 103  LTSFADVTHCFILGDVTYGACCVDDLSAVALGADLLIHYGHSCLVPIDATKIPCLYVFVE 162

Query: 1051 IAINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSA 872
            I IN   L++ + HN    K  + V+AGTIQFS+AIR+ KPELEK GFRVLIPQSKPLSA
Sbjct: 163  IKINVDGLVNTIKHNLGSSK-ESIVLAGTIQFSSAIRSAKPELEKRGFRVLIPQSKPLSA 221

Query: 871  GEVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY 692
            GEVLGCTAP +        ++V +FVADGRFHLEAFMIANP I+AFRYDPYLGKLFLEEY
Sbjct: 222  GEVLGCTAPKIG-SIANANESVAVFVADGRFHLEAFMIANPGIKAFRYDPYLGKLFLEEY 280

Query: 691  DHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISEL 512
            DH+GM+  RR+AIE+AK AK+WGIVLGTLGRQGNP  L RLEGKM++KG DY V+L+SE+
Sbjct: 281  DHKGMRETRRRAIEKAKSAKSWGIVLGTLGRQGNPKVLERLEGKMKEKGYDYTVILMSEI 340

Query: 511  SPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERK--LEC 338
            SP +I LF DAVDAW+QIACPRLSIDWGDAF KPLLT+FEAEIALG++ GWWE+     C
Sbjct: 341  SPVRIALFEDAVDAWVQIACPRLSIDWGDAFLKPLLTTFEAEIALGEIPGWWEKDGCGSC 400

Query: 337  NGRLDGSKKEGCCGNENVQKGV--DYPMDYYAQDGGEWNSCYSKKPARDWGRKI 182
               L  S+ + CCG+ N  K    DYPMDYYAQ+GGEWNS Y KK  R   R I
Sbjct: 401  GNGLSCSESKSCCGDGNGMKDFHGDYPMDYYAQNGGEWNSSYVKKSVRLMRRNI 454


>gb|KHF97888.1| Diphthamide biosynthesis 1 [Gossypium arboreum]
          Length = 567

 Score =  490 bits (1262), Expect = e-136
 Identities = 242/348 (69%), Positives = 279/348 (80%), Gaps = 4/348 (1%)
 Frame = -2

Query: 1231 LTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVE 1052
            LT+FA VTHCFILGDVTYGACCVDDLSA AL   LL+H+GHSCLVPID+T IP LY+FVE
Sbjct: 102  LTSFADVTHCFILGDVTYGACCVDDLSAVALGADLLIHYGHSCLVPIDATKIPCLYVFVE 161

Query: 1051 IAINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSA 872
            I IN   L++ + HN    K  + V+AGTIQFS+AIR+ KPELEK GFRVLIPQSKPLSA
Sbjct: 162  IKINVDGLVNTIEHNLGSSK-ESIVLAGTIQFSSAIRSAKPELEKRGFRVLIPQSKPLSA 220

Query: 871  GEVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY 692
            GEVLGCTAP +        ++V +FVADGRFHLEAFMIANP I+AFRYDPYLGKLFLEEY
Sbjct: 221  GEVLGCTAPKIG-SIANANESVAVFVADGRFHLEAFMIANPGIKAFRYDPYLGKLFLEEY 279

Query: 691  DHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISEL 512
            DH+GM+  RR+AIE+AK AK+WGIVLGTLGRQGNP  L RLEGKM++KG DY V+L+SE+
Sbjct: 280  DHKGMRETRRRAIEKAKSAKSWGIVLGTLGRQGNPKVLERLEGKMKEKGYDYTVILMSEI 339

Query: 511  SPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERK--LEC 338
            SP +I LF DAVDAW+QIACPRLSIDWGDAF KPLLT+FEAEIALG+++GWWE+     C
Sbjct: 340  SPVRIALFEDAVDAWVQIACPRLSIDWGDAFLKPLLTTFEAEIALGEISGWWEKDGFGSC 399

Query: 337  NGRLDGSKKEGCCGNENVQKGV--DYPMDYYAQDGGEWNSCYSKKPAR 200
               L   + + CCG+ N  K    DYPMDYYAQDGGEWNS Y KK  R
Sbjct: 400  GNGLSCRESKSCCGDGNGMKDFHGDYPMDYYAQDGGEWNSSYVKKSVR 447


>ref|XP_011010116.1| PREDICTED: diphthamide biosynthesis protein 1 [Populus euphratica]
            gi|743931672|ref|XP_011010117.1| PREDICTED: diphthamide
            biosynthesis protein 1 [Populus euphratica]
          Length = 460

 Score =  489 bits (1258), Expect = e-135
 Identities = 250/372 (67%), Positives = 286/372 (76%), Gaps = 21/372 (5%)
 Frame = -2

Query: 1228 TTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVEI 1049
            T FA VTHCF+LGDVTYGACCVDDLSA ALD  LL+H+GHSCL+PID+T IP LY+FVEI
Sbjct: 82   TAFADVTHCFVLGDVTYGACCVDDLSALALDADLLIHYGHSCLIPIDATKIPCLYVFVEI 141

Query: 1048 AINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSAG 869
             I+  +L++ ++ NF   K  N V+AGTIQF++AIR  KPELE  GF VLIPQSKPLSAG
Sbjct: 142  KIDVSRLINTISLNFGTHK-RNIVLAGTIQFASAIREAKPELENNGFVVLIPQSKPLSAG 200

Query: 868  EVLGCTAPSVKLQDCAG-----KDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLF 704
            EVLGCTAP +            ++ V++FVADGRFHLEAFMIANP I+AFRYDPYLGKLF
Sbjct: 201  EVLGCTAPKMSKNVVGNLGFSEEEMVLVFVADGRFHLEAFMIANPGIKAFRYDPYLGKLF 260

Query: 703  LEEYDHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVL 524
            LEEYDH+GMK  R+KAIERAKGA++WG+VLGTLGRQGNP  L RLE KM +KG DYMVVL
Sbjct: 261  LEEYDHKGMKEVRKKAIERAKGARSWGVVLGTLGRQGNPRILERLEKKMGEKGYDYMVVL 320

Query: 523  ISELSPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKL 344
            +SE+SP +I LF D+VDAWIQIACPRLSIDWGDAFKKPLLT FEAEIALGDLAGWWE+ L
Sbjct: 321  MSEISPSRIALFEDSVDAWIQIACPRLSIDWGDAFKKPLLTPFEAEIALGDLAGWWEKSL 380

Query: 343  ECNGRLD--------GSKKEG-CCGNEN-----VQK--GVDYPMDYYAQDGGEWNSCYSK 212
              N   D        G K +G CC   N     V+K  G +YPMDYYAQDGGEWNS Y K
Sbjct: 381  VVNSGCDGGGSGLGCGQKSDGSCCECSNGDAKGVEKDFGGEYPMDYYAQDGGEWNSSYVK 440

Query: 211  KPARDWGRKIQS 176
            KP R   R + S
Sbjct: 441  KPTRPVRRNVVS 452


>gb|AIU49158.1| diphthamide synthesis DPH2 family protein, partial [Erythranthe
            guttata]
          Length = 341

 Score =  486 bits (1251), Expect = e-134
 Identities = 257/331 (77%), Positives = 265/331 (80%)
 Frame = -2

Query: 1201 FILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVEIAINTRKLLH 1022
            F+LGDVTYGACCVDDLSARALD HLLVHFGHSCLVPIDSTTIPVLYIFVEIAINTRKLLH
Sbjct: 70   FVLGDVTYGACCVDDLSARALDAHLLVHFGHSCLVPIDSTTIPVLYIFVEIAINTRKLLH 129

Query: 1021 ELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSAGEVLGCTAPS 842
            ELNHNF        VMAGTIQFSNAIRAVKPEL      VLIPQSKPLSAGEVLGCTAPS
Sbjct: 130  ELNHNF--------VMAGTIQFSNAIRAVKPEL------VLIPQSKPLSAGEVLGCTAPS 175

Query: 841  VKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEYDHEGMKGDRR 662
            V           IIFVADGRFHLEAFMIANPEIRAFR+DPYLGKLFLEEYDHEGMKGDRR
Sbjct: 176  V-----------IIFVADGRFHLEAFMIANPEIRAFRFDPYLGKLFLEEYDHEGMKGDRR 224

Query: 661  KAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISELSPKKIELFGD 482
            KAIERA GAKTWGIVLGTLGRQGN + + RL   M+DKG   MVVLISELSPKKIELFGD
Sbjct: 225  KAIERAMGAKTWGIVLGTLGRQGNTMIMKRL---MKDKG---MVVLISELSPKKIELFGD 278

Query: 481  AVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLECNGRLDGSKKEGC 302
            AVDAW+QIACPRLSIDWGDAFKKPLLT+FEAEIALGDL GWW+                 
Sbjct: 279  AVDAWVQIACPRLSIDWGDAFKKPLLTAFEAEIALGDLDGWWQ----------------- 321

Query: 301  CGNENVQKGVDYPMDYYAQDGGEWNSCYSKK 209
                       YPMDYYAQDGG WNSCYSKK
Sbjct: 322  -----------YPMDYYAQDGGGWNSCYSKK 341


>ref|XP_006280451.1| hypothetical protein CARUB_v10026383mg [Capsella rubella]
            gi|482549155|gb|EOA13349.1| hypothetical protein
            CARUB_v10026383mg [Capsella rubella]
          Length = 453

 Score =  481 bits (1239), Expect = e-133
 Identities = 242/359 (67%), Positives = 283/359 (78%), Gaps = 16/359 (4%)
 Frame = -2

Query: 1228 TTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVEI 1049
            T+FA  +HCF+LGDVTYGACCVDD SA AL   LL+H+GHSCLVPIDST IP LY+FVEI
Sbjct: 86   TSFAGASHCFVLGDVTYGACCVDDFSACALGADLLIHYGHSCLVPIDSTKIPCLYVFVEI 145

Query: 1048 AINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSAG 869
             I+ + LL+ ++ N + D   N ++AGTIQF++AIRAVKPELEK GF VLIPQSKPLSAG
Sbjct: 146  QIDVKCLLNTIHLNLSSDA-KNIILAGTIQFTSAIRAVKPELEKQGFNVLIPQSKPLSAG 204

Query: 868  EVLGCTAPSVK-LQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY 692
            EVLGCTAP +K ++DC  KD V++FVADGRFHLEAFMIANP+I+AFRYDPYLGKLFLEEY
Sbjct: 205  EVLGCTAPKIKTVEDC--KDQVLVFVADGRFHLEAFMIANPKIKAFRYDPYLGKLFLEEY 262

Query: 691  DHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISEL 512
            DH+GM+  RR+AI RA+ AKTWGIVLGTLGRQGNP  L RLE KMR+KG+D  VVL+SEL
Sbjct: 263  DHKGMRETRRRAIARARDAKTWGIVLGTLGRQGNPKILERLEKKMREKGIDSTVVLMSEL 322

Query: 511  SPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLECNG 332
            SP ++ LF D+VDAW+QIACPRLSIDWG+AF KPLLT+FEAEIALG + GWWE+     G
Sbjct: 323  SPTRVALFEDSVDAWVQIACPRLSIDWGEAFLKPLLTTFEAEIALGFIRGWWEKDTSSRG 382

Query: 331  -RLDGSKKEG-----CCGNENVQKGV---------DYPMDYYAQDGGEWNSCYSKKPAR 200
              L G  KE       C NENV+            DYPMDYYAQ+GGEWNS Y KK +R
Sbjct: 383  DSLSGCCKEKKETSCACSNENVENDKKDDDGALDGDYPMDYYAQEGGEWNSSYLKKSSR 441


>dbj|BAB10164.1| unnamed protein product [Arabidopsis thaliana]
          Length = 468

 Score =  474 bits (1221), Expect = e-131
 Identities = 240/359 (66%), Positives = 281/359 (78%), Gaps = 16/359 (4%)
 Frame = -2

Query: 1228 TTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVEI 1049
            T+FA  +HCF+LGDVTYGACCVDD SA AL   LL+H+GHSCLVPIDST IP LY+FVEI
Sbjct: 101  TSFAGASHCFVLGDVTYGACCVDDFSACALGADLLIHYGHSCLVPIDSTKIPCLYVFVEI 160

Query: 1048 AINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSAG 869
             I+ + LL+ ++ N   D + N ++AGTIQF++AIRAVKPELEK GF VLIPQSKPLSAG
Sbjct: 161  QIDVKCLLNTIHLNLASD-VKNIILAGTIQFTSAIRAVKPELEKQGFNVLIPQSKPLSAG 219

Query: 868  EVLGCTAPSVK-LQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY 692
            EVLGCTAP VK ++DC  KD V++FVADGRFHLEAFMIANP+I+AFRYDPYLGKLFLEEY
Sbjct: 220  EVLGCTAPKVKTVEDC--KDQVLVFVADGRFHLEAFMIANPKIKAFRYDPYLGKLFLEEY 277

Query: 691  DHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISEL 512
            DH+GM+  R +AI RA+ AKTWGIVLGTLGRQGNP  L RLE KM +KG+D  VVL+SEL
Sbjct: 278  DHKGMRETRMRAIARAREAKTWGIVLGTLGRQGNPKILERLEKKMMEKGIDSTVVLMSEL 337

Query: 511  SPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWE-----RK 347
            SP ++ LF D+VDAW+QIACPRLSIDWG+AF KPLLT+FEAEIALG + GWWE     R 
Sbjct: 338  SPTRVALFEDSVDAWVQIACPRLSIDWGEAFLKPLLTTFEAEIALGFIRGWWENDSSSRV 397

Query: 346  LECNGRLDGSKKEGC-CGNENVQKGV---------DYPMDYYAQDGGEWNSCYSKKPAR 200
               +G     K+  C C NENV+            DYPMDYYAQ+GGEWNS Y KK +R
Sbjct: 398  NSSSGCCKEDKETSCACKNENVKDDKKDNDGALDGDYPMDYYAQEGGEWNSSYLKKSSR 456


>ref|NP_201009.1| diphthamide synthesis DPH2 family protein [Arabidopsis thaliana]
            gi|20259528|gb|AAM13884.1| unknown protein [Arabidopsis
            thaliana] gi|21436215|gb|AAM51395.1| unknown protein
            [Arabidopsis thaliana] gi|110742463|dbj|BAE99150.1|
            hypothetical protein [Arabidopsis thaliana]
            gi|332010170|gb|AED97553.1| diphthamide synthesis DPH2
            family protein [Arabidopsis thaliana]
          Length = 453

 Score =  474 bits (1221), Expect = e-131
 Identities = 240/359 (66%), Positives = 281/359 (78%), Gaps = 16/359 (4%)
 Frame = -2

Query: 1228 TTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVEI 1049
            T+FA  +HCF+LGDVTYGACCVDD SA AL   LL+H+GHSCLVPIDST IP LY+FVEI
Sbjct: 86   TSFAGASHCFVLGDVTYGACCVDDFSACALGADLLIHYGHSCLVPIDSTKIPCLYVFVEI 145

Query: 1048 AINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSAG 869
             I+ + LL+ ++ N   D + N ++AGTIQF++AIRAVKPELEK GF VLIPQSKPLSAG
Sbjct: 146  QIDVKCLLNTIHLNLASD-VKNIILAGTIQFTSAIRAVKPELEKQGFNVLIPQSKPLSAG 204

Query: 868  EVLGCTAPSVK-LQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEY 692
            EVLGCTAP VK ++DC  KD V++FVADGRFHLEAFMIANP+I+AFRYDPYLGKLFLEEY
Sbjct: 205  EVLGCTAPKVKTVEDC--KDQVLVFVADGRFHLEAFMIANPKIKAFRYDPYLGKLFLEEY 262

Query: 691  DHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISEL 512
            DH+GM+  R +AI RA+ AKTWGIVLGTLGRQGNP  L RLE KM +KG+D  VVL+SEL
Sbjct: 263  DHKGMRETRMRAIARAREAKTWGIVLGTLGRQGNPKILERLEKKMMEKGIDSTVVLMSEL 322

Query: 511  SPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWE-----RK 347
            SP ++ LF D+VDAW+QIACPRLSIDWG+AF KPLLT+FEAEIALG + GWWE     R 
Sbjct: 323  SPTRVALFEDSVDAWVQIACPRLSIDWGEAFLKPLLTTFEAEIALGFIRGWWENDSSSRV 382

Query: 346  LECNGRLDGSKKEGC-CGNENVQKGV---------DYPMDYYAQDGGEWNSCYSKKPAR 200
               +G     K+  C C NENV+            DYPMDYYAQ+GGEWNS Y KK +R
Sbjct: 383  NSSSGCCKEDKETSCACKNENVKDDKKDNDGALDGDYPMDYYAQEGGEWNSSYLKKSSR 441


>ref|XP_002864766.1| diphthamide synthesis DPH2 family protein [Arabidopsis lyrata subsp.
            lyrata] gi|297310601|gb|EFH41025.1| diphthamide synthesis
            DPH2 family protein [Arabidopsis lyrata subsp. lyrata]
          Length = 453

 Score =  473 bits (1218), Expect = e-130
 Identities = 237/358 (66%), Positives = 279/358 (77%), Gaps = 15/358 (4%)
 Frame = -2

Query: 1228 TTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVEI 1049
            T+FA  +HCF+LGDVTYGACCVDD SA AL   LL+H+GHSCLVPIDST IP LY+FVEI
Sbjct: 86   TSFAGASHCFVLGDVTYGACCVDDFSACALGADLLIHYGHSCLVPIDSTKIPCLYVFVEI 145

Query: 1048 AINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSAG 869
             I+ + LL+ ++ N   D   N ++AGTIQF++AIRAVKPELEK GF V+IPQSKPLSAG
Sbjct: 146  QIDVKCLLNTIHMNLASDA-KNIILAGTIQFTSAIRAVKPELEKQGFNVMIPQSKPLSAG 204

Query: 868  EVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEYD 689
            EVLGCTAP +K  +  GKD V++FVADGRFHLEAFMIANP+I+AFRYDPYLGKLFLEEYD
Sbjct: 205  EVLGCTAPKIKTVE-DGKDQVLVFVADGRFHLEAFMIANPKIKAFRYDPYLGKLFLEEYD 263

Query: 688  HEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISELS 509
            H+GM+  RR+AI RA+ AKTWGIVLGTLGRQGNP+ L RLE KM +KG+D  VVL+SELS
Sbjct: 264  HKGMRETRRRAIARARDAKTWGIVLGTLGRQGNPMILERLEKKMVEKGIDSTVVLMSELS 323

Query: 508  PKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWE-----RKL 344
            P ++ LF D+VDAW+QIACPRLSIDWG+AF KPLLT+FEAEIALG + GWWE     R  
Sbjct: 324  PTRVALFEDSVDAWVQIACPRLSIDWGEAFLKPLLTTFEAEIALGFIRGWWENGSSSRVE 383

Query: 343  ECNGRLDGSKKEGC-CGNENVQKGV---------DYPMDYYAQDGGEWNSCYSKKPAR 200
              +G     K+  C C NE V+            DYPMDYYAQ+GGEWNS Y KK +R
Sbjct: 384  SSSGCCKEDKERSCACKNEKVEDDKKDNDGALDGDYPMDYYAQEGGEWNSSYLKKSSR 441


>ref|XP_010458072.1| PREDICTED: diphthamide biosynthesis protein 1-like [Camelina sativa]
            gi|727426025|ref|XP_010458080.1| PREDICTED: diphthamide
            biosynthesis protein 1-like [Camelina sativa]
            gi|727426027|ref|XP_010458087.1| PREDICTED: diphthamide
            biosynthesis protein 1-like [Camelina sativa]
          Length = 458

 Score =  473 bits (1217), Expect = e-130
 Identities = 238/359 (66%), Positives = 279/359 (77%), Gaps = 16/359 (4%)
 Frame = -2

Query: 1228 TTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVEI 1049
            T+FA   HCF+LGDVTYGACCVDD SA AL   LL+H+GHSCLVPIDST IP LY+FV+I
Sbjct: 86   TSFAGALHCFVLGDVTYGACCVDDFSAVALGADLLIHYGHSCLVPIDSTKIPCLYVFVDI 145

Query: 1048 AINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSAG 869
             I+ + LL+ ++ N T D   N V+AGTIQFS+AIRAVKP+LEKLGF VLIPQSKPLSAG
Sbjct: 146  QIDVKCLLNTIHLNLTSDA-KNIVLAGTIQFSSAIRAVKPDLEKLGFNVLIPQSKPLSAG 204

Query: 868  EVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEYD 689
            EVLGCTAP +K+ +   +D V++FVADGRFHLEA MIANP+I+AFRYDPYLGKLFLEEYD
Sbjct: 205  EVLGCTAPKIKMVEGC-EDQVLVFVADGRFHLEAIMIANPKIKAFRYDPYLGKLFLEEYD 263

Query: 688  HEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISELS 509
            H+GM+  RR+AI RA  AKTWGIVLGTLGRQGNP  L RLE KMR+ G+D  VVL+SELS
Sbjct: 264  HKGMRETRRRAIARAMDAKTWGIVLGTLGRQGNPKILERLETKMRENGIDSTVVLMSELS 323

Query: 508  PKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLECNG- 332
            P ++ LF D+VDAW+QIACPRLSIDWG+AF KPLLT++EAEIALG ++GWWE+     G 
Sbjct: 324  PTRVALFEDSVDAWVQIACPRLSIDWGEAFLKPLLTTYEAEIALGYISGWWEKDTSSRGD 383

Query: 331  RLDGSKKE------GCCGNENVQK---------GVDYPMDYYAQDGGEWNSCYSKKPAR 200
             L G  KE       C  NENV+            DYPMDYYAQ+GGEWNS Y KK +R
Sbjct: 384  SLAGCCKEKNETSCACSSNENVENDNKEDDGALDGDYPMDYYAQEGGEWNSSYLKKASR 442


>ref|XP_002325444.1| diphthamide synthesis DPH2 family protein [Populus trichocarpa]
            gi|222862319|gb|EEE99825.1| diphthamide synthesis DPH2
            family protein [Populus trichocarpa]
          Length = 428

 Score =  473 bits (1217), Expect = e-130
 Identities = 241/356 (67%), Positives = 276/356 (77%), Gaps = 5/356 (1%)
 Frame = -2

Query: 1228 TTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVEI 1049
            T FA VTHCF+LGDVTYGACCVDDLSA ALD  LL+H+GHSCL+PID+T IP LY+FVEI
Sbjct: 82   TAFADVTHCFVLGDVTYGACCVDDLSALALDADLLIHYGHSCLIPIDATKIPCLYVFVEI 141

Query: 1048 AINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSAG 869
             I+  +L++ ++ NF   K  N V+AGTIQF++AIR  KPELE  GF VLIPQSKPLSAG
Sbjct: 142  KIDVSRLINTISLNFGTHK-RNIVLAGTIQFASAIREAKPELENNGFVVLIPQSKPLSAG 200

Query: 868  EVLGCTAPSVKLQDCAG-----KDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLF 704
            EVLGCTAP +            ++ V++FVADGRFHLEAFMIANP I+AFRYDPYLGKLF
Sbjct: 201  EVLGCTAPKMSKNVVGNLGFSEEEMVLVFVADGRFHLEAFMIANPGIKAFRYDPYLGKLF 260

Query: 703  LEEYDHEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVL 524
            LEEYDH+GMK  R+KAIERAKGA++WG+VLGTLGRQGNP  L RLE KM +KG DYMVVL
Sbjct: 261  LEEYDHKGMKEVRKKAIERAKGARSWGVVLGTLGRQGNPRILERLEKKMGEKGFDYMVVL 320

Query: 523  ISELSPKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKL 344
            +SE+SP +I LF D+VDAWIQIACPRLSIDWGDAFKKPLLT FEAEIALGDLAG      
Sbjct: 321  MSEISPGRIALFEDSVDAWIQIACPRLSIDWGDAFKKPLLTPFEAEIALGDLAG------ 374

Query: 343  ECNGRLDGSKKEGCCGNENVQKGVDYPMDYYAQDGGEWNSCYSKKPARDWGRKIQS 176
             CNG   G +K+          G +YPMDYYAQDGGEWNS Y KKP R   R + S
Sbjct: 375  -CNGDAKGVEKD---------FGGEYPMDYYAQDGGEWNSSYVKKPTRPVRRNVVS 420


>ref|XP_013630682.1| PREDICTED: diphthamide biosynthesis protein 1 [Brassica oleracea var.
            oleracea] gi|922457679|ref|XP_013630683.1| PREDICTED:
            diphthamide biosynthesis protein 1 [Brassica oleracea
            var. oleracea] gi|922457682|ref|XP_013630684.1|
            PREDICTED: diphthamide biosynthesis protein 1 [Brassica
            oleracea var. oleracea]
          Length = 451

 Score =  471 bits (1212), Expect = e-130
 Identities = 238/360 (66%), Positives = 277/360 (76%), Gaps = 17/360 (4%)
 Frame = -2

Query: 1228 TTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDSTTIPVLYIFVEI 1049
            T+FA  +HCF+LGDVTYGACCVDD SA AL   LL+H+GHSCLVPIDST IP LY+FVEI
Sbjct: 86   TSFAGASHCFVLGDVTYGACCVDDFSASALGADLLIHYGHSCLVPIDSTKIPCLYVFVEI 145

Query: 1048 AINTRKLLHELNHNFTPDKINNFVMAGTIQFSNAIRAVKPELEKLGFRVLIPQSKPLSAG 869
             I+ + LL  ++ N + D + + ++AGTIQF++AIRAVKPELEK GF VLIPQSKPLSAG
Sbjct: 146  QIDVKCLLSTIHLNLSSDDVKSIILAGTIQFTSAIRAVKPELEKQGFSVLIPQSKPLSAG 205

Query: 868  EVLGCTAPSVKLQDCAGKDNVIIFVADGRFHLEAFMIANPEIRAFRYDPYLGKLFLEEYD 689
            EVLGCTAP V   D   KD V++FVADGRFHLEAFMIANP+I+AFRYDPYLGKLFLEEYD
Sbjct: 206  EVLGCTAPKVTRVD-DHKDAVLVFVADGRFHLEAFMIANPKIKAFRYDPYLGKLFLEEYD 264

Query: 688  HEGMKGDRRKAIERAKGAKTWGIVLGTLGRQGNPIALNRLEGKMRDKGLDYMVVLISELS 509
            H+GM+  RR+AI RAK AKTWGIVLGTLGRQGNP  L RLE KM +KG+D  VVL+SELS
Sbjct: 265  HKGMRETRRRAITRAKDAKTWGIVLGTLGRQGNPKILERLEKKMEEKGIDSTVVLMSELS 324

Query: 508  PKKIELFGDAVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDLAGWWERKLECNGR 329
            P ++ LF D+VDAW+QIACPRLSIDWG+AF KPLLT+FEAEIALG + GWWE+     G 
Sbjct: 325  PTRVALFEDSVDAWVQIACPRLSIDWGEAFLKPLLTTFEAEIALGFIPGWWEK-----GS 379

Query: 328  LD--GSKKEGCC---------------GNENVQKGVDYPMDYYAQDGGEWNSCYSKKPAR 200
            L    S   GCC                ++ V  G DYPMDYYAQ+GGEWNS Y KK +R
Sbjct: 380  LSRAESSSSGCCKEDKETSCACRDDKKDDDGVLDG-DYPMDYYAQEGGEWNSSYLKKSSR 438