BLASTX nr result
ID: Papaver32_contig00025405
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver32_contig00025405
(1354 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AOR51553.1 putative N-methyltransferase [Papaver somniferum] 531 0.0
ANY58201.1 putative N-methyltransferase [synthetic construct] 508 e-177
ANY58193.1 putative N-methyltransferase [synthetic construct] 506 e-176
C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 496 e-172
ANY58195.1 TNMT [synthetic construct] 495 e-172
ANY58202.1 putative N-methyltransferase [synthetic construct] 486 e-169
ANY58191.1 putative N-methyltransferase [synthetic construct] 481 e-166
Q108P1.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 477 e-165
ANY58190.1 putative N-methyltransferase [synthetic construct] 477 e-165
C3SBU5.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 475 e-164
ANY58200.1 putative N-methyltransferase [synthetic construct] 471 e-162
ANY58196.1 putative N-methyltransferase [synthetic construct] 464 e-160
ANY58198.1 putative N-methyltransferase [synthetic construct] 459 e-157
C3SBU4.1 RecName: Full=Probable (S)-tetrahydroprotoberberine N-m... 445 e-152
ANY58192.1 putative N-methyltransferase [synthetic construct] 417 e-141
AKN62838.1 (S)-tetrahydroprotoberberine-cis-N-methyltransferase,... 399 e-135
5KN4_A Chain A, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KN4... 367 e-121
C3SBW0.1 RecName: Full=Pavine N-methyltransferase; Short=PfPavNM... 365 e-121
AJD20224.1 coclaurine N-methyltransferase [Sinopodophyllum hexan... 365 e-121
5KPC_A Chain A, Pavine N-methyltransferase H206a Mutant In Compl... 363 e-119
>AOR51553.1 putative N-methyltransferase [Papaver somniferum]
Length = 356
Score = 531 bits (1367), Expect = 0.0
Identities = 262/344 (76%), Positives = 297/344 (86%)
Frame = +2
Query: 125 ENGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMS 304
++G KILERLV G+IGDEELK+LI+IRFEK LQ GYKPT QDQL S + FIKSLK+M+MS
Sbjct: 6 QHGTKILERLVKGEIGDEELKELIRIRFEKRLQCGYKPTPQDQLASEMAFIKSLKDMKMS 65
Query: 305 GDLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDG 484
G+LE +N+E YELPTA + A LG+TLKQSACYF++ESM++DEAEIAAYEL+CERA+I+DG
Sbjct: 66 GELEAVNTELYELPTAAVAATLGSTLKQSACYFKEESMSIDEAEIAAYELDCERAQIKDG 125
Query: 485 QTVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTK 664
QT+LDIGCG GGLV HIAQKYKN HVTG+TNS QRNYI+ Q+EKL L+NVDVILADVTK
Sbjct: 126 QTILDIGCGFGGLVLHIAQKYKNSHVTGLTNSAEQRNYIMLQVEKLSLSNVDVILADVTK 185
Query: 665 FEFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALD 844
EF EKKFDRILV+EAIEHMKNIQLF+KKIS WMKDE SFLFV HLCH AF+ HFEALD
Sbjct: 186 HEFENEKKFDRILVVEAIEHMKNIQLFLKKISNWMKDEDSFLFVVHLCHKAFSQHFEALD 245
Query: 845 EDDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDV 1024
EDDWYTSYVLP+GSVT LS +ALLYFQDDVSVVD WLL+G HMARSQEEW K+ NL+
Sbjct: 246 EDDWYTSYVLPEGSVTYLSASALLYFQDDVSVVDQWLLSGTHMARSQEEWFKRFKINLEA 305
Query: 1025 AKKELKQGLGSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMIS 1156
A K L GLGSEEAANQV +TFF+G VQFSFNNGEEWMIS
Sbjct: 306 ASKALTVGLGSEEAANQVNIQYKTFFMGYVVQFSFNNGEEWMIS 349
>ANY58201.1 putative N-methyltransferase [synthetic construct]
Length = 365
Score = 508 bits (1308), Expect = e-177
Identities = 240/347 (69%), Positives = 297/347 (85%)
Frame = +2
Query: 119 MGENGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEME 298
+ G+I+ RL+ G+IGD+ELKKLI+ +E+ LQWGY+PT QL+ N+DFI+SLK+M+
Sbjct: 16 LSARAGEIMGRLMKGEIGDDELKKLIRNMWERRLQWGYQPTHHQQLSFNLDFIRSLKKMD 75
Query: 299 MSGDLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIE 478
MSG++ETMN ETYELPTAFLEAA G T+KQSACYF+D+S TLDEAE A++EL CERA+I+
Sbjct: 76 MSGEIETMNEETYELPTAFLEAAFGKTIKQSACYFKDKSTTLDEAEEASHELYCERAQIK 135
Query: 479 DGQTVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADV 658
DGQTVLD+GCGQGGLV HIAQKYKNC VTGITNS AQ NYI+ Q EKL+L+NVDVILADV
Sbjct: 136 DGQTVLDVGCGQGGLVLHIAQKYKNCRVTGITNSKAQMNYIVMQAEKLQLSNVDVILADV 195
Query: 659 TKFEFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEA 838
TKF+ +++ +DRILVIE IEHMKNIQLFMKK+S W+K + S LFV+H+CH F+HHFE
Sbjct: 196 TKFD--SDQTYDRILVIETIEHMKNIQLFMKKLSTWLKKD-SLLFVDHICHKTFSHHFET 252
Query: 839 LDEDDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNL 1018
LDE+DWY+ ++ PKG VT+ S +ALLYFQDDVSVVDHW++NGMHMARS EEW KKLDKN+
Sbjct: 253 LDEEDWYSGFIFPKGCVTLPSASALLYFQDDVSVVDHWVVNGMHMARSVEEWGKKLDKNM 312
Query: 1019 DVAKKELKQGLGSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
+ A++ L+ GLGS+EA NQVITH+RTF +GG QFSFNNGEEWM++Q
Sbjct: 313 EAAREILEPGLGSKEAVNQVITHIRTFCIGGYEQFSFNNGEEWMVAQ 359
>ANY58193.1 putative N-methyltransferase [synthetic construct]
Length = 360
Score = 506 bits (1302), Expect = e-176
Identities = 240/342 (70%), Positives = 293/342 (85%)
Frame = +2
Query: 134 GKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMSGDL 313
G+I+ RL+ G+IGDEEL KL++ ++E+ LQWGYKPT + QL N DFIKSLK+M+MSG++
Sbjct: 16 GEIMGRLMKGEIGDEELSKLVRHQWERRLQWGYKPTHEQQLACNFDFIKSLKDMDMSGEI 75
Query: 314 ETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDGQTV 493
+TMN ETYELP+AFLEA G T+KQS CYF+DE+ TLDEAE A++EL CERA+I+DGQT+
Sbjct: 76 DTMNEETYELPSAFLEATFGKTIKQSGCYFKDETTTLDEAEEASHELYCERAQIKDGQTI 135
Query: 494 LDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTKFEF 673
LDIGCGQGGLV +IAQKYKNCHVTG+TNS AQ NYIL Q +KL+L+NVDVI ADVTKF+
Sbjct: 136 LDIGCGQGGLVLYIAQKYKNCHVTGLTNSKAQMNYILMQAKKLQLSNVDVISADVTKFD- 194
Query: 674 VTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALDEDD 853
+K +DRILVIEAIEHMKNIQLF+KK+S WMK + S LFV+H+ H FNHHFEALDE+D
Sbjct: 195 -NDKTYDRILVIEAIEHMKNIQLFLKKLSTWMKKD-SLLFVDHISHKKFNHHFEALDEED 252
Query: 854 WYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDVAKK 1033
WY+ ++ PKG VTILS + LLYFQDDVSVVDHW++NGMHMARS EEW KKLDKN++ A++
Sbjct: 253 WYSGFIFPKGCVTILSSSTLLYFQDDVSVVDHWVVNGMHMARSMEEWRKKLDKNMEAARE 312
Query: 1034 ELKQGLGSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
L+ GLGS+EA NQVITH+RTF +GG QFSFNNGEEWMI+Q
Sbjct: 313 ILEPGLGSKEAVNQVITHIRTFCLGGYEQFSFNNGEEWMITQ 354
>C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT ACO90222.1 tetrahydroprotoberberine
N-methyltransferase [Eschscholzia californica]
Length = 350
Score = 496 bits (1277), Expect = e-172
Identities = 236/347 (68%), Positives = 290/347 (83%)
Frame = +2
Query: 119 MGENGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEME 298
MG + G+I+ RL+ G+I DEELKKLI+ ++++ ++WGYKPT + QL N+DFIK LKEM
Sbjct: 1 MGSSAGEIMGRLMKGEIEDEELKKLIRHQWDRRIEWGYKPTHEKQLAFNLDFIKGLKEMV 60
Query: 299 MSGDLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIE 478
MSG+++TMN ETYELPTAFLEA G T+KQS CYF+DE+ T+DEAE AA+EL CERA+I+
Sbjct: 61 MSGEIDTMNKETYELPTAFLEAVFGKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIK 120
Query: 479 DGQTVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADV 658
DGQTVLDIGCGQGGLV +IA+KYKNCHVTG+TNS AQ NYI Q EKL+LTNVDVI ADV
Sbjct: 121 DGQTVLDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADV 180
Query: 659 TKFEFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEA 838
TKF+ T+K +DRILV+E IEHMKNIQLFMKK+S WM E S LFV+H+ H FNH+FEA
Sbjct: 181 TKFD--TDKTYDRILVVETIEHMKNIQLFMKKLSTWM-TEDSLLFVDHISHKTFNHNFEA 237
Query: 839 LDEDDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNL 1018
LDEDDWY+ ++ PKG VTILS + LLYFQDDVS +DHW++NGMHMARS E W KKLD+ +
Sbjct: 238 LDEDDWYSGFIFPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEAWRKKLDETI 297
Query: 1019 DVAKKELKQGLGSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
+ A++ L+ GLGS+EA NQVITH+RTF +GG QFS+NNGEEWMI+Q
Sbjct: 298 EAAREILEPGLGSKEAVNQVITHIRTFCIGGYEQFSYNNGEEWMITQ 344
>ANY58195.1 TNMT [synthetic construct]
Length = 350
Score = 495 bits (1274), Expect = e-172
Identities = 236/347 (68%), Positives = 288/347 (82%)
Frame = +2
Query: 119 MGENGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEME 298
MG + G+I+ RL+ G+I DEELKKLI+ ++++ +QWGYKPT + QL N+DFIK LKEM
Sbjct: 1 MGSSAGEIMGRLMKGEIEDEELKKLIRHQWDRRIQWGYKPTHEKQLAFNLDFIKGLKEMV 60
Query: 299 MSGDLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIE 478
MSG+++TMN ETYELPTAFLEA G T+KQS CYF+DE+ T+DEAE AA+EL CERA+I+
Sbjct: 61 MSGEIDTMNKETYELPTAFLEAVFGKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIK 120
Query: 479 DGQTVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADV 658
DGQTVLDIGCGQGGLV +IA+KYKNCHVTG+TNS AQ NYI Q EKL+L NVDVI ADV
Sbjct: 121 DGQTVLDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELANVDVIFADV 180
Query: 659 TKFEFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEA 838
TKF+ T+K +DRILV+E IEHMKNIQLFMKK+S WM E S LFV+H+ H FNH+FEA
Sbjct: 181 TKFD--TDKTYDRILVVETIEHMKNIQLFMKKLSTWM-TEDSLLFVDHISHKTFNHNFEA 237
Query: 839 LDEDDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNL 1018
LDEDDWY+ ++ PKG VTILS + LLYFQDDVS +DHW++NGMHMARS E W KKLD +
Sbjct: 238 LDEDDWYSGFIFPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEAWRKKLDNTI 297
Query: 1019 DVAKKELKQGLGSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
+ A++ L+ GLGS+EA NQVITH+RTF +GG QFS+NNGEEWMI+Q
Sbjct: 298 EAAREILEPGLGSKEAVNQVITHIRTFCIGGYEQFSYNNGEEWMITQ 344
>ANY58202.1 putative N-methyltransferase [synthetic construct]
Length = 333
Score = 486 bits (1251), Expect = e-169
Identities = 234/323 (72%), Positives = 277/323 (85%)
Frame = +2
Query: 128 NGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMSG 307
N GKI+ERL+ G+IGDEEL KL++ +E+ LQWGYKPT + QL N DFIKSLK M+MSG
Sbjct: 14 NAGKIMERLMKGEIGDEELSKLVRHMWERRLQWGYKPTHEQQLAFNFDFIKSLKHMDMSG 73
Query: 308 DLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDGQ 487
+++TMN ETYELPTAFLEAA G T+KQS CYF+DE+ TLDEAE A++EL CERA+I+DGQ
Sbjct: 74 EIDTMNEETYELPTAFLEAAFGKTIKQSGCYFKDETTTLDEAEEASHELYCERAQIKDGQ 133
Query: 488 TVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTKF 667
+VLDIGCGQGGLV HIAQKYKNCHVTG+TNS AQ NYIL Q EKL+L+NVDVI ADVTKF
Sbjct: 134 SVLDIGCGQGGLVLHIAQKYKNCHVTGLTNSKAQMNYILMQAEKLQLSNVDVISADVTKF 193
Query: 668 EFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALDE 847
+ T+K +DRILVIEAIEHMKNIQLFMKK+S WMK E S LFV+H+ H FNHHFEALDE
Sbjct: 194 D--TDKTYDRILVIEAIEHMKNIQLFMKKLSTWMK-EDSLLFVDHISHKTFNHHFEALDE 250
Query: 848 DDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDVA 1027
+DWY+ ++ PKG VTILS + LLYFQDDVSVVDHW++NGMHMARS EEW KKLDKN++ A
Sbjct: 251 EDWYSGFIFPKGCVTILSSSTLLYFQDDVSVVDHWVVNGMHMARSVEEWRKKLDKNMEAA 310
Query: 1028 KKELKQGLGSEEAANQVITHLRT 1096
++ L+ GLGS+EA NQVITH+RT
Sbjct: 311 REILEPGLGSKEAVNQVITHIRT 333
>ANY58191.1 putative N-methyltransferase [synthetic construct]
Length = 350
Score = 481 bits (1238), Expect = e-166
Identities = 232/344 (67%), Positives = 283/344 (82%), Gaps = 1/344 (0%)
Frame = +2
Query: 131 GGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMSGD 310
G LERL ++ DEELKK I+I +EK LQWGY+ T +DQL SN+DF+KSLKEM+MSGD
Sbjct: 2 GINFLERLGKREVSDEELKKQIRIIWEKRLQWGYRSTHEDQLKSNLDFVKSLKEMKMSGD 61
Query: 311 LETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDGQT 490
++T N+E+YELP+ FLE+ G T+KQSACYF+ ESMT+DEAE A++EL CERA+I+DGQT
Sbjct: 62 IDTFNNESYELPSEFLESMFGKTIKQSACYFKHESMTIDEAEDASHELYCERAQIKDGQT 121
Query: 491 VLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTKFE 670
VLDIGCG GGLV +IAQKYKNCHVTG+TNS AQ NYI Q +KL LTNV+ ILADVTK E
Sbjct: 122 VLDIGCGHGGLVLYIAQKYKNCHVTGLTNSRAQVNYIEMQSKKLNLTNVEAILADVTKHE 181
Query: 671 FVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALDED 850
F TEK FDRI++IEAIEHMKNI+LFMKK+S WM +E S LFV+H+CH FNHHFEA+DE+
Sbjct: 182 FKTEKTFDRIILIEAIEHMKNIELFMKKLSTWMTEE-SLLFVDHICHKTFNHHFEAMDEE 240
Query: 851 DWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDVAK 1030
DWY+ Y+ PKG VTILS +ALLYFQ DVSVVDHW++NG HMARS +EW KKLD N++ +
Sbjct: 241 DWYSGYIFPKGCVTILSSSALLYFQSDVSVVDHWVVNGNHMARSVQEWLKKLDDNMETVR 300
Query: 1031 KELKQGL-GSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
L+ + GS+EA +QVITH RTF +G QFSFNNGEEWM++Q
Sbjct: 301 GILEPAMGGSKEAVDQVITHSRTFCMGAYEQFSFNNGEEWMVAQ 344
>Q108P1.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT AAY79177.1
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-cis-
N-methyltransferase [Papaver somniferum]
Length = 358
Score = 477 bits (1228), Expect = e-165
Identities = 232/346 (67%), Positives = 282/346 (81%), Gaps = 1/346 (0%)
Frame = +2
Query: 125 ENGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMS 304
E+ G+ L RL+ G+I DEELKKLIK +FEK LQWGYK + Q+QL+ N+DFIKSLK+MEMS
Sbjct: 10 ESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLKKMEMS 69
Query: 305 GDLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDG 484
G++ETMN ETYELP+ FLEA G T+KQS CYF ES T+DEAE AA+EL CERA+I+DG
Sbjct: 70 GEIETMNKETYELPSEFLEAVFGKTVKQSMCYFTHESATIDEAEEAAHELYCERAQIKDG 129
Query: 485 QTVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTK 664
QTVLDIGCGQGGLV +IAQKYKNCHVTG+TNS AQ NY+L Q EKL LTNVD ILADVT+
Sbjct: 130 QTVLDIGCGQGGLVLYIAQKYKNCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQ 189
Query: 665 FEFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALD 844
+E ++K +DR+L+IEAIEHMKN+QLFMKK+S WM E S LFV+H+CH F H FEA+D
Sbjct: 190 YE--SDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTKE-SLLFVDHVCHKTFAHFFEAVD 246
Query: 845 EDDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDV 1024
EDDWY+ ++ P G TIL+ +LLYFQDDVSVVDHW++NGMHMARS + W K LDKN++
Sbjct: 247 EDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEA 306
Query: 1025 AKKELKQGL-GSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
AK+ L GL GS E N V+TH+RTF +GG QFS NNG+EWM++Q
Sbjct: 307 AKEILLPGLGGSHETVNGVVTHIRTFCMGGYEQFSMNNGDEWMVAQ 352
>ANY58190.1 putative N-methyltransferase [synthetic construct]
Length = 362
Score = 477 bits (1228), Expect = e-165
Identities = 231/342 (67%), Positives = 283/342 (82%), Gaps = 1/342 (0%)
Frame = +2
Query: 137 KILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMSGDLE 316
+IL RL+ G+I DEEL+KLIK +F+K L+WGYK T QL+ N+DFIKSLK M+MSG++E
Sbjct: 18 EILGRLLKGEIKDEELRKLIKFQFQKRLEWGYKSTHHQQLSFNLDFIKSLKNMDMSGEIE 77
Query: 317 TMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDGQTVL 496
TMN ETYELPT FLEAA G T+KQS CYF+ ES T+DEAE A++EL CERA+I+DGQTVL
Sbjct: 78 TMNKETYELPTEFLEAAFGKTMKQSGCYFKHESTTIDEAEEASHELYCERAQIKDGQTVL 137
Query: 497 DIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTKFEFV 676
DIGCGQGGLV +IA+KYK CHVTG+TNS AQ NYI Q +KL L+NVDVILADVTK++
Sbjct: 138 DIGCGQGGLVLYIAEKYKKCHVTGLTNSKAQVNYIEMQAKKLGLSNVDVILADVTKYD-- 195
Query: 677 TEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALDEDDW 856
++K +DR+L+IEAIEHMKNIQLFMKK+S WM + S LFV+H+CH F+H FEA+DEDDW
Sbjct: 196 SDKTYDRLLMIEAIEHMKNIQLFMKKLSTWMTKD-SLLFVDHICHKTFSHPFEAVDEDDW 254
Query: 857 YTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDVAKKE 1036
Y+ ++ P G VTILS ALLYFQDDVSVVDHW++NGMHMARS E W KKLDKN+ AK+
Sbjct: 255 YSGFIFPPGCVTILSSDALLYFQDDVSVVDHWVINGMHMARSVEAWRKKLDKNMKAAKEI 314
Query: 1037 LKQGL-GSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
L GL G+ EA NQ++TH+RTF +GG QFS+NNGEEWM++Q
Sbjct: 315 LLPGLGGNHEAVNQIVTHIRTFCMGGYEQFSYNNGEEWMVAQ 356
>C3SBU5.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 ACO90237.1 tetrahydroprotoberberine
N-methyltransferase [Papaver bracteatum]
Length = 358
Score = 475 bits (1222), Expect = e-164
Identities = 230/346 (66%), Positives = 284/346 (82%), Gaps = 1/346 (0%)
Frame = +2
Query: 125 ENGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMS 304
E+ G+ L RL+ G+I DEELKKLIK +FEK LQWGYK + Q+QL+ N+DFIKSLK+MEMS
Sbjct: 10 ESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLKKMEMS 69
Query: 305 GDLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDG 484
G++ETMN ETYELP+ FLEA G T+KQS CYF+ ES T+DEAE AA+EL CERA+I+DG
Sbjct: 70 GEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATIDEAEEAAHELYCERAQIKDG 129
Query: 485 QTVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTK 664
QTVLDIGCGQGGLV +IA+KYK CHVTG+TNS AQ NY+L Q EKL LTNVD ILADVT+
Sbjct: 130 QTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQ 189
Query: 665 FEFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALD 844
+E ++K +DR+L+IEAIEHMKN+QLFMKK+S WM +E S LFV+H+CH F H FEA+D
Sbjct: 190 YE--SDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEE-SLLFVDHVCHKTFAHFFEAVD 246
Query: 845 EDDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDV 1024
EDDWY+ ++ P G TIL+ +LLYFQDDVSVVDHW++NGMHMARS + W K LDKN++
Sbjct: 247 EDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEA 306
Query: 1025 AKKELKQGL-GSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
AK+ L GL GS EA N V+TH+RTF +GG QFS N+G+EWM++Q
Sbjct: 307 AKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQ 352
>ANY58200.1 putative N-methyltransferase [synthetic construct]
Length = 358
Score = 471 bits (1213), Expect = e-162
Identities = 230/345 (66%), Positives = 281/345 (81%), Gaps = 1/345 (0%)
Frame = +2
Query: 125 ENGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMS 304
E+ + L RL+ G+I DEELKKLIK + EK LQWGYK + Q+QL+ N+DFIKSLK+MEMS
Sbjct: 10 ESAEETLGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFIKSLKKMEMS 69
Query: 305 GDLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDG 484
G++ETMN ETYELP+ FLEA G T+KQS CYF+ ES T+DEAE AA+EL CERA+I+DG
Sbjct: 70 GEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESSTIDEAEEAAHELYCERAQIKDG 129
Query: 485 QTVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTK 664
QTVLDIGCGQGGLV +IAQKYK CHVTG+TNS Q NYIL Q EKL L NVDV+LADVT+
Sbjct: 130 QTVLDIGCGQGGLVLYIAQKYKKCHVTGLTNSKEQVNYILKQAEKLGLRNVDVMLADVTQ 189
Query: 665 FEFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALD 844
+E ++K +DR+L IE IEHMKNIQLFMKK+S WM E S LFVEH+CH F+H FEA+D
Sbjct: 190 YE--SDKTYDRLLTIELIEHMKNIQLFMKKLSTWM-TEDSLLFVEHVCHKTFSHFFEAID 246
Query: 845 EDDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDV 1024
EDDWY+ ++ P GSVTIL+ +LLYFQD+VSVVDHW++NGMH ARS + W KK+DKN++V
Sbjct: 247 EDDWYSGFIFPPGSVTILAANSLLYFQDEVSVVDHWVVNGMHTARSFDAWRKKVDKNMEV 306
Query: 1025 AKKELKQGL-GSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMIS 1156
AK+ L GL GS EA N V+TH+RTF +GG QFS NNG+EWM++
Sbjct: 307 AKEILLPGLGGSHEAVNAVVTHIRTFCMGGYEQFSLNNGDEWMVA 351
>ANY58196.1 putative N-methyltransferase [synthetic construct]
Length = 357
Score = 464 bits (1194), Expect = e-160
Identities = 221/340 (65%), Positives = 278/340 (81%)
Frame = +2
Query: 137 KILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMSGDLE 316
+ILERL G I DEEL++L+KI++ + L+WGYK T QL N+DF+KSLK+M+MSG +E
Sbjct: 14 EILERLDKGLIPDEELRELVKIQWGRRLKWGYKSTHVQQLEFNLDFVKSLKQMDMSGKME 73
Query: 317 TMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDGQTVL 496
+NSE+YELP AFL A G TLKQS CYFEDESMTLDEAE AA+EL CERA+I+DGQ VL
Sbjct: 74 VINSESYELPVAFLRAMFGKTLKQSCCYFEDESMTLDEAETAAHELYCERAQIKDGQRVL 133
Query: 497 DIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTKFEFV 676
DIGCGQG L+ HIAQKYKNCHVTGITNS AQ+N I + LKL+NV+VILADVT+++
Sbjct: 134 DIGCGQGSLIIHIAQKYKNCHVTGITNSNAQKNIIEEECRNLKLSNVEVILADVTQYD-- 191
Query: 677 TEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALDEDDW 856
T+ FDRI++IEA+EHMKNI LF+KKISKWMKD+G +F++ +CH F HHFEA+DEDDW
Sbjct: 192 TKDTFDRIILIEAVEHMKNIDLFLKKISKWMKDDG-LMFIDCICHKTFGHHFEAIDEDDW 250
Query: 857 YTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDVAKKE 1036
Y+ Y+ PKGSVT+ + + LLYFQ+DV+VVD W ++G HMAR+ EEW KKLDKN+DVA++
Sbjct: 251 YSGYIFPKGSVTLQAASTLLYFQEDVAVVDQWAVSGKHMARTVEEWLKKLDKNIDVAREI 310
Query: 1037 LKQGLGSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMIS 1156
L+ LGS+EA +VITH RTF +G + QFS+NNG+EWMIS
Sbjct: 311 LEPSLGSKEAVEKVITHSRTFCIGTSEQFSYNNGDEWMIS 350
>ANY58198.1 putative N-methyltransferase [synthetic construct]
Length = 359
Score = 459 bits (1180), Expect = e-157
Identities = 226/346 (65%), Positives = 275/346 (79%), Gaps = 1/346 (0%)
Frame = +2
Query: 125 ENGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMS 304
E+ + L RL+ G+I DEELKKLIK + EK LQWGYK + Q+QL+ N+DFIKSLK+MEMS
Sbjct: 10 ESAEETLGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFIKSLKKMEMS 69
Query: 305 GDLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDG 484
G++ETMN ETYELP+ FLEA G T+KQS CYF+ ES T+DEAE AA+EL CERA+I+DG
Sbjct: 70 GEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESSTIDEAEEAAHELYCERAQIKDG 129
Query: 485 QTVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTK 664
QTVLDIGCGQGGLV +IAQKYK CHVTG+TNS Q NYIL Q EKL L NVDV+LADVT+
Sbjct: 130 QTVLDIGCGQGGLVLYIAQKYKKCHVTGLTNSKEQVNYILKQAEKLGLRNVDVMLADVTQ 189
Query: 665 FEFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALD 844
+E ++K +DRILVI +EH+KN+QLFMKK+S WM E S LFV+H CH FNH FEALD
Sbjct: 190 YE--SDKTYDRILVIGFVEHIKNLQLFMKKLSTWMA-EDSLLFVDHSCHKTFNHFFEALD 246
Query: 845 EDDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDV 1024
EDDWY+ Y+ P G T LS +LLYFQDDVSVVDHW++NGMH AR+ + W KKLDKN++
Sbjct: 247 EDDWYSGYIFPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDAWRKKLDKNMEA 306
Query: 1025 AKKELKQGL-GSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
K+ L GL G+ EA N VITH++T VGG VQFS ++G+EWM +Q
Sbjct: 307 VKEILLPGLGGNHEAVNGVITHIKTCCVGGYVQFSLSDGDEWMNAQ 352
>C3SBU4.1 RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2 ACO90236.1 putative
N-methyltransferase [Papaver bracteatum] ANY58199.1 TNMT2
[synthetic construct]
Length = 358
Score = 445 bits (1145), Expect = e-152
Identities = 220/346 (63%), Positives = 268/346 (77%), Gaps = 1/346 (0%)
Frame = +2
Query: 125 ENGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMS 304
E+ + L RL+ G+I DEELKKLIK + EK LQWGYK + Q+QL+ N+DFI SLK+M MS
Sbjct: 10 ESAEETLGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFINSLKKMGMS 69
Query: 305 GDLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDG 484
G +E +E YELPT EAA G ++K S CYF+ ES T+DEAE A++EL CERA+I+DG
Sbjct: 70 GQVEAFTNEVYELPTECFEAAYGKSMKLSGCYFKHESSTIDEAEEASHELYCERAQIKDG 129
Query: 485 QTVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTK 664
QTVLDIGCGQGGLV ++AQKYKNCHVTG+TNS Q NYIL Q EKL L NVDVILADVT+
Sbjct: 130 QTVLDIGCGQGGLVLYVAQKYKNCHVTGLTNSKEQVNYILKQAEKLGLRNVDVILADVTQ 189
Query: 665 FEFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALD 844
+E ++K +DRILVI +EHMKN+QLF+KK+S WM E S LFV+H CH FNH FEALD
Sbjct: 190 YE--SDKTYDRILVIGVVEHMKNMQLFIKKLSTWMA-EDSLLFVDHSCHKTFNHFFEALD 246
Query: 845 EDDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDV 1024
EDDWY+ Y+ P G T LS +LLYFQDDVSVVDHW++NGMH AR+ + W KKLDKN++
Sbjct: 247 EDDWYSGYIFPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDAWRKKLDKNMEA 306
Query: 1025 AKKELKQGL-GSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
K+ L GL G+ EA N VITH+RT VGG VQFS N+G+EWM +Q
Sbjct: 307 VKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDGDEWMNAQ 352
>ANY58192.1 putative N-methyltransferase [synthetic construct]
Length = 353
Score = 417 bits (1073), Expect = e-141
Identities = 203/343 (59%), Positives = 266/343 (77%), Gaps = 3/343 (0%)
Frame = +2
Query: 140 ILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMSGDLET 319
ILER +IGDEE K+ I+ + K LQWGY+ T ++QL SN+DFIKSLK+M+MSGD++
Sbjct: 6 ILERFRKREIGDEEFKQHIRSIWVKRLQWGYRSTHEEQLNSNLDFIKSLKKMDMSGDIDK 65
Query: 320 MNSETYELPTAFLEAALGNTLKQSACYFEDESMT--LDEAEIAAYELNCERAKIEDGQTV 493
N++ YELP+ F + G ++KQS CYF++ES T LDEA ++EL CERA+I+DGQTV
Sbjct: 66 YNTQEYELPSEFQQIIFGKSIKQSGCYFKEESKTKTLDEATDDSHELYCERAQIKDGQTV 125
Query: 494 LDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTKFEF 673
LDIGCG GGLV +IAQKYKNCHVTG+TNS AQ +I Q +KL LTNV+VILADVTK F
Sbjct: 126 LDIGCGNGGLVLYIAQKYKNCHVTGLTNSKAQVKHIELQTKKLNLTNVEVILADVTKHVF 185
Query: 674 VTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALDEDD 853
TEK FDRI++I+ IEHMKNI+LFM+K+S WM +E S LFV+H+CH FN+HFE +DE+D
Sbjct: 186 KTEKTFDRIILIQVIEHMKNIELFMRKLSTWMTEE-SLLFVDHICHKTFNYHFEPMDEND 244
Query: 854 WYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDVAKK 1033
WY+ ++ P+G VTILS ++LLYFQ DVSV+DHW++NG HMARS +EW K LD N++ ++
Sbjct: 245 WYSGHIFPEGCVTILSSSSLLYFQKDVSVLDHWVVNGKHMARSAQEWLKSLDDNMEKVRE 304
Query: 1034 ELKQGL-GSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
L+ + G++EA +QV+T R F +G QFS NNGEEWM +Q
Sbjct: 305 ILEPSMGGNKEAVDQVLTQSRFFCMGAYEQFSNNNGEEWMFAQ 347
>AKN62838.1 (S)-tetrahydroprotoberberine-cis-N-methyltransferase, partial
[Papaver bracteatum]
Length = 283
Score = 399 bits (1024), Expect = e-135
Identities = 191/284 (67%), Positives = 233/284 (82%)
Frame = +2
Query: 218 LQWGYKPTLQDQLTSNIDFIKSLKEMEMSGDLETMNSETYELPTAFLEAALGNTLKQSAC 397
LQWGYK + Q+QL+ N+DFIKSLK+MEMSG++ETMN ETYELP+ FLEA G T+KQS C
Sbjct: 2 LQWGYKSSHQEQLSFNLDFIKSLKKMEMSGEIETMNKETYELPSEFLEAVFGKTVKQSMC 61
Query: 398 YFEDESMTLDEAEIAAYELNCERAKIEDGQTVLDIGCGQGGLVFHIAQKYKNCHVTGITN 577
YF+ ES T+DEAE AA+EL CERA+I+DGQTVLDIGCGQGGLV +IA+KYK CHVTG+TN
Sbjct: 62 YFKHESATIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTN 121
Query: 578 STAQRNYILSQIEKLKLTNVDVILADVTKFEFVTEKKFDRILVIEAIEHMKNIQLFMKKI 757
S AQ NY+L Q EKL LTNVD ILADVT++E ++K +DR+L+IEAIEHMKN+QLFMKK+
Sbjct: 122 SKAQVNYLLKQAEKLGLTNVDAILADVTQYE--SDKTYDRLLMIEAIEHMKNLQLFMKKL 179
Query: 758 SKWMKDEGSFLFVEHLCHTAFNHHFEALDEDDWYTSYVLPKGSVTILSLTALLYFQDDVS 937
S WM +E S LFV+H+CH F H FEA+DEDDWY+ ++ P G TIL+ +LLYFQDDVS
Sbjct: 180 STWMTEE-SLLFVDHVCHKTFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVS 238
Query: 938 VVDHWLLNGMHMARSQEEWAKKLDKNLDVAKKELKQGLGSEEAA 1069
VVDHW++NGMHMARS + W K LDKN++ AK+ L GLG A
Sbjct: 239 VVDHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHEA 282
>5KN4_A Chain A, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KN4_B Chain B,
Pavine N-methyltransferase Apoenzyme Ph 6.0 5KOC_A Chain
A, Pavine N-methyltransferase In Complex With
S-adenosylmethionine Ph 7 5KOC_B Chain B, Pavine
N-methyltransferase In Complex With S-adenosylmethionine
Ph 7 5KOK_A Chain A, Pavine N-methyltransferase In
Complex With Tetrahydropapaverine And S-
Adenosylhomocysteine Ph 7.25 5KOK_B Chain B, Pavine
N-methyltransferase In Complex With Tetrahydropapaverine
And S- Adenosylhomocysteine Ph 7.25 5KPG_A Chain A,
Pavine N-methyltransferase In Complex With
S-adenosylhomocysteine Ph 7 5KPG_B Chain B, Pavine
N-methyltransferase In Complex With
S-adenosylhomocysteine Ph 7
Length = 397
Score = 367 bits (942), Expect = e-121
Identities = 170/340 (50%), Positives = 245/340 (72%)
Frame = +2
Query: 140 ILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMSGDLET 319
+++R+ +G++ DEE++ ++KI+ +K L+WGYKPT + QL + F +SLK MEM+ +++T
Sbjct: 55 LIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGMEMAEEVDT 114
Query: 320 MNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDGQTVLD 499
+++E YE+P FL G TLK S YF+DES TLDE+E+ +L CERA+I+DGQ++LD
Sbjct: 115 LDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQIKDGQSILD 174
Query: 500 IGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTKFEFVT 679
+GCG G L H+AQKY+ C VTGITNS +Q+ +I+ Q +KL L+NV++IL DVTKFE T
Sbjct: 175 LGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDVTKFE--T 232
Query: 680 EKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALDEDDWY 859
E +DRI + IEHMKN +LF+KK+S W+ +G LFVEH CH F + +E LDEDDWY
Sbjct: 233 EITYDRIFAVALIEHMKNYELFLKKVSTWIAQDG-LLFVEHHCHKVFAYQYEPLDEDDWY 291
Query: 860 TSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDVAKKEL 1039
T Y+ P G++ + S + LLYFQ+DVSVV+HW L+G H + ++W K+LD N+D K+
Sbjct: 292 TEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEIF 351
Query: 1040 KQGLGSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
+ GS+E A + IT+ R F + + +S NNGEEWM+SQ
Sbjct: 352 ESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQ 391
>C3SBW0.1 RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT ACO90251.1
pavine N-methyltransferase [Thalictrum flavum] ANY58204.1
PNMT [synthetic construct]
Length = 356
Score = 365 bits (938), Expect = e-121
Identities = 170/340 (50%), Positives = 244/340 (71%)
Frame = +2
Query: 140 ILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMSGDLET 319
+++R+ +G++ DEE++ ++KI+ +K L+WGYKPT + QL + F +SLK MEM+ +++T
Sbjct: 14 LIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGMEMAEEVDT 73
Query: 320 MNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDGQTVLD 499
+++E YE+P FL G TLK S YF+DES TLDE+E+ +L CERA+I+DGQ++LD
Sbjct: 74 LDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQIKDGQSILD 133
Query: 500 IGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTKFEFVT 679
+GCG G L H+AQKY+ C VTGITNS +Q+ +I+ Q +KL L+NV++IL DVTKFE T
Sbjct: 134 LGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDVTKFE--T 191
Query: 680 EKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALDEDDWY 859
E +DRI + IEHMKN +LF+KK+S W+ G LFVEH CH F + +E LDEDDWY
Sbjct: 192 EITYDRIFAVALIEHMKNYELFLKKVSTWIAQYG-LLFVEHHCHKVFAYQYEPLDEDDWY 250
Query: 860 TSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDVAKKEL 1039
T Y+ P G++ + S + LLYFQ+DVSVV+HW L+G H + ++W K+LD N+D K+
Sbjct: 251 TEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEIF 310
Query: 1040 KQGLGSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
+ GS+E A + IT+ R F + + +S NNGEEWM+SQ
Sbjct: 311 ESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQ 350
>AJD20224.1 coclaurine N-methyltransferase [Sinopodophyllum hexandrum]
Length = 370
Score = 365 bits (938), Expect = e-121
Identities = 175/343 (51%), Positives = 236/343 (68%)
Frame = +2
Query: 128 NGGKILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMSG 307
N ++L +L G + D E+K+LI+ ++ L+WGYK T Q QL +DF SL+ M ++
Sbjct: 24 NKAELLRKLELGVVPDHEIKQLIRHELDRRLRWGYKSTHQQQLAQLVDFAHSLRRMNIAD 83
Query: 308 DLETMNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDGQ 487
+++T++S YE+P +FL G LK S CYF +S TL EAEIA +L CERA++ DG
Sbjct: 84 EVDTLDSNIYEVPISFLRIMNGPMLKGSGCYFPHDSTTLAEAEIAMLDLYCERAQVRDGH 143
Query: 488 TVLDIGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTKF 667
T+LD+GCGQG L HIA+KY NC VT ITNS +Q+++I Q K LTNV+V+LAD+TK
Sbjct: 144 TILDLGCGQGALTLHIAKKYTNCRVTAITNSVSQKHFIQDQCIKSNLTNVEVLLADITKH 203
Query: 668 EFVTEKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALDE 847
+ T FDR+ VIE +EHMKN +L +KKISKWM +G LF+EH+CH F +H++ LDE
Sbjct: 204 D--TSHSFDRVFVIELLEHMKNYELLLKKISKWMSQDG-LLFIEHICHKTFAYHYDPLDE 260
Query: 848 DDWYTSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDVA 1027
DDW+T YV P G++ I S + LLYFQDDVSVV+HW L+G H +R+ EEW K+LD N+D
Sbjct: 261 DDWFTDYVFPAGTMIIPSASFLLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANVDTI 320
Query: 1028 KKELKQGLGSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMIS 1156
K + LGSEEAA ++I + + F G F NNGEEWM S
Sbjct: 321 KAIFEASLGSEEAAVKLINYWKGFCFSGMEMFGSNNGEEWMTS 363
>5KPC_A Chain A, Pavine N-methyltransferase H206a Mutant In Complex With S-
Adenosylmethionine Ph 6 5KPC_B Chain B, Pavine
N-methyltransferase H206a Mutant In Complex With S-
Adenosylmethionine Ph 6
Length = 397
Score = 363 bits (932), Expect = e-119
Identities = 169/340 (49%), Positives = 244/340 (71%)
Frame = +2
Query: 140 ILERLVNGDIGDEELKKLIKIRFEKILQWGYKPTLQDQLTSNIDFIKSLKEMEMSGDLET 319
+++R+ +G++ DEE++ ++KI+ +K L+WGYKPT + QL + F +SLK MEM+ +++T
Sbjct: 55 LIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGMEMAEEVDT 114
Query: 320 MNSETYELPTAFLEAALGNTLKQSACYFEDESMTLDEAEIAAYELNCERAKIEDGQTVLD 499
+++E YE+P FL G TLK S YF+DES TLDE+E+ +L CERA+I+DGQ++LD
Sbjct: 115 LDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQIKDGQSILD 174
Query: 500 IGCGQGGLVFHIAQKYKNCHVTGITNSTAQRNYILSQIEKLKLTNVDVILADVTKFEFVT 679
+GCG G L H+AQKY+ C VTGITNS +Q+ +I+ Q +KL L+NV++IL DVTKFE T
Sbjct: 175 LGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDVTKFE--T 232
Query: 680 EKKFDRILVIEAIEHMKNIQLFMKKISKWMKDEGSFLFVEHLCHTAFNHHFEALDEDDWY 859
E +DRI + IE MKN +LF+KK+S W+ +G LFVEH CH F + +E LDEDDWY
Sbjct: 233 EITYDRIFAVALIEAMKNYELFLKKVSTWIAQDG-LLFVEHHCHKVFAYQYEPLDEDDWY 291
Query: 860 TSYVLPKGSVTILSLTALLYFQDDVSVVDHWLLNGMHMARSQEEWAKKLDKNLDVAKKEL 1039
T Y+ P G++ + S + LLYFQ+DVSVV+HW L+G H + ++W K+LD N+D K+
Sbjct: 292 TEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEIF 351
Query: 1040 KQGLGSEEAANQVITHLRTFFVGGAVQFSFNNGEEWMISQ 1159
+ GS+E A + IT+ R F + + +S NNGEEWM+SQ
Sbjct: 352 ESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQ 391