BLASTX nr result
ID: Papaver32_contig00007929
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver32_contig00007929
(768 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
C3SBU4.1 RecName: Full=Probable (S)-tetrahydroprotoberberine N-m... 301 7e-99
ANY58198.1 putative N-methyltransferase [synthetic construct] 296 1e-96
C3SBU5.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 285 2e-92
Q108P1.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 284 6e-92
ANY58200.1 putative N-methyltransferase [synthetic construct] 282 3e-91
ANY58190.1 putative N-methyltransferase [synthetic construct] 278 9e-90
ANY58195.1 TNMT [synthetic construct] 261 3e-83
C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 261 3e-83
ANY58201.1 putative N-methyltransferase [synthetic construct] 261 4e-83
ANY58193.1 putative N-methyltransferase [synthetic construct] 259 4e-82
ANY58191.1 putative N-methyltransferase [synthetic construct] 251 5e-79
AKN62838.1 (S)-tetrahydroprotoberberine-cis-N-methyltransferase,... 244 3e-77
ANY58192.1 putative N-methyltransferase [synthetic construct] 230 5e-71
ANY58202.1 putative N-methyltransferase [synthetic construct] 226 1e-69
ANY58196.1 putative N-methyltransferase [synthetic construct] 213 2e-64
AOR51553.1 putative N-methyltransferase [Papaver somniferum] 207 4e-62
5KN4_A Chain A, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KN4... 182 6e-52
C3SBW0.1 RecName: Full=Pavine N-methyltransferase; Short=PfPavNM... 179 5e-51
5KPC_A Chain A, Pavine N-methyltransferase H206a Mutant In Compl... 179 2e-50
BAB71802.1 coclaurine N-methyltransferase [Coptis japonica] 175 2e-49
>C3SBU4.1 RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2 ACO90236.1 putative
N-methyltransferase [Papaver bracteatum] ANY58199.1
TNMT2 [synthetic construct]
Length = 358
Score = 301 bits (772), Expect = 7e-99
Identities = 149/207 (71%), Positives = 157/207 (75%), Gaps = 38/207 (18%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNI 587
NCHVTGLTNSKEQVNYI+KQ EKLGL+NVDV+LADVTQYESDKTYDRILVIG VEHMKN+
Sbjct: 152 NCHVTGLTNSKEQVNYILKQAEKLGLRNVDVILADVTQYESDKTYDRILVIGVVEHMKNM 211
Query: 586 QLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLY 407
QLF+KKLSTWM EDS LFVDH CHKTF+H FEA+DEDDWYSGYIF PGC T LSADSLLY
Sbjct: 212 QLFIKKLSTWMAEDSLLFVDHSCHKTFNHFFEALDEDDWYSGYIFPPGCATFLSADSLLY 271
Query: 406 FQDDVSVVDHWV--------------------------------------XNGVITHIRT 341
FQDDVSVVDHWV NGVITHIRT
Sbjct: 272 FQDDVSVVDHWVVNGMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRT 331
Query: 340 LCLGGYVQFSFNDGDEWMNAQLLFKKK 260
C+GGYVQFS NDGDEWMNAQLLFKKK
Sbjct: 332 CCVGGYVQFSLNDGDEWMNAQLLFKKK 358
>ANY58198.1 putative N-methyltransferase [synthetic construct]
Length = 359
Score = 296 bits (757), Expect = 1e-96
Identities = 146/206 (70%), Positives = 156/206 (75%), Gaps = 38/206 (18%)
Frame = -3
Query: 763 CHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNIQ 584
CHVTGLTNSKEQVNYI+KQ EKLGL+NVDV+LADVTQYESDKTYDRILVIG VEH+KN+Q
Sbjct: 153 CHVTGLTNSKEQVNYILKQAEKLGLRNVDVMLADVTQYESDKTYDRILVIGFVEHIKNLQ 212
Query: 583 LFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLYF 404
LFMKKLSTWM EDS LFVDH CHKTF+H FEA+DEDDWYSGYIF PGC T LSADSLLYF
Sbjct: 213 LFMKKLSTWMAEDSLLFVDHSCHKTFNHFFEALDEDDWYSGYIFPPGCATFLSADSLLYF 272
Query: 403 QDDVSVVDHWV--------------------------------------XNGVITHIRTL 338
QDDVSVVDHWV NGVITHI+T
Sbjct: 273 QDDVSVVDHWVVNGMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIKTC 332
Query: 337 CLGGYVQFSFNDGDEWMNAQLLFKKK 260
C+GGYVQFS +DGDEWMNAQLLFKKK
Sbjct: 333 CVGGYVQFSLSDGDEWMNAQLLFKKK 358
>C3SBU5.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 ACO90237.1 tetrahydroprotoberberine
N-methyltransferase [Papaver bracteatum]
Length = 358
Score = 285 bits (729), Expect = 2e-92
Identities = 137/206 (66%), Positives = 154/206 (74%), Gaps = 38/206 (18%)
Frame = -3
Query: 763 CHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNIQ 584
CHVTGLTNSK QVNY++KQ EKLGL NVD +LADVTQYESDKTYDR+L+I A+EHMKN+Q
Sbjct: 153 CHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQ 212
Query: 583 LFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLYF 404
LFMKKLSTWMTE+S LFVDH+CHKTF+H FEA+DEDDWYSG+IF PGC T+L+A+SLLYF
Sbjct: 213 LFMKKLSTWMTEESLLFVDHVCHKTFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYF 272
Query: 403 QDDVSVVDHWV--------------------------------------XNGVITHIRTL 338
QDDVSVVDHWV NGV+THIRT
Sbjct: 273 QDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTF 332
Query: 337 CLGGYVQFSFNDGDEWMNAQLLFKKK 260
C+GGY QFS NDGDEWM AQLLFKKK
Sbjct: 333 CMGGYEQFSMNDGDEWMVAQLLFKKK 358
>Q108P1.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT AAY79177.1
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
cis-N-methyltransferase [Papaver somniferum]
Length = 358
Score = 284 bits (726), Expect = 6e-92
Identities = 136/207 (65%), Positives = 155/207 (74%), Gaps = 38/207 (18%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNI 587
NCHVTGLTNSK QVNY++KQ EKLGL NVD +LADVTQYESDKTYDR+L+I A+EHMKN+
Sbjct: 152 NCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNL 211
Query: 586 QLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLY 407
QLFMKKLSTWMT++S LFVDH+CHKTF+H FEA+DEDDWYSG+IF PGC T+L+A+SLLY
Sbjct: 212 QLFMKKLSTWMTKESLLFVDHVCHKTFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLY 271
Query: 406 FQDDVSVVDHWV--------------------------------------XNGVITHIRT 341
FQDDVSVVDHWV NGV+THIRT
Sbjct: 272 FQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHETVNGVVTHIRT 331
Query: 340 LCLGGYVQFSFNDGDEWMNAQLLFKKK 260
C+GGY QFS N+GDEWM AQLLFKKK
Sbjct: 332 FCMGGYEQFSMNNGDEWMVAQLLFKKK 358
>ANY58200.1 putative N-methyltransferase [synthetic construct]
Length = 358
Score = 282 bits (722), Expect = 3e-91
Identities = 138/206 (66%), Positives = 154/206 (74%), Gaps = 38/206 (18%)
Frame = -3
Query: 763 CHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNIQ 584
CHVTGLTNSKEQVNYI+KQ EKLGL+NVDV+LADVTQYESDKTYDR+L I +EHMKNIQ
Sbjct: 153 CHVTGLTNSKEQVNYILKQAEKLGLRNVDVMLADVTQYESDKTYDRLLTIELIEHMKNIQ 212
Query: 583 LFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLYF 404
LFMKKLSTWMTEDS LFV+H+CHKTFSH FEAIDEDDWYSG+IF PG +T+L+A+SLLYF
Sbjct: 213 LFMKKLSTWMTEDSLLFVEHVCHKTFSHFFEAIDEDDWYSGFIFPPGSVTILAANSLLYF 272
Query: 403 QDDVSVVDHWVXNG--------------------------------------VITHIRTL 338
QD+VSVVDHWV NG V+THIRT
Sbjct: 273 QDEVSVVDHWVVNGMHTARSFDAWRKKVDKNMEVAKEILLPGLGGSHEAVNAVVTHIRTF 332
Query: 337 CLGGYVQFSFNDGDEWMNAQLLFKKK 260
C+GGY QFS N+GDEWM A LLFKKK
Sbjct: 333 CMGGYEQFSLNNGDEWMVAHLLFKKK 358
>ANY58190.1 putative N-methyltransferase [synthetic construct]
Length = 362
Score = 278 bits (712), Expect = 9e-90
Identities = 133/206 (64%), Positives = 155/206 (75%), Gaps = 38/206 (18%)
Frame = -3
Query: 763 CHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNIQ 584
CHVTGLTNSK QVNYI Q +KLGL NVDV+LADVT+Y+SDKTYDR+L+I A+EHMKNIQ
Sbjct: 157 CHVTGLTNSKAQVNYIEMQAKKLGLSNVDVILADVTKYDSDKTYDRLLMIEAIEHMKNIQ 216
Query: 583 LFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLYF 404
LFMKKLSTWMT+DS LFVDH+CHKTFSH FEA+DEDDWYSG+IF PGC+T+LS+D+LLYF
Sbjct: 217 LFMKKLSTWMTKDSLLFVDHICHKTFSHPFEAVDEDDWYSGFIFPPGCVTILSSDALLYF 276
Query: 403 QDDVSVVDHWVXNG--------------------------------------VITHIRTL 338
QDDVSVVDHWV NG ++THIRT
Sbjct: 277 QDDVSVVDHWVINGMHMARSVEAWRKKLDKNMKAAKEILLPGLGGNHEAVNQIVTHIRTF 336
Query: 337 CLGGYVQFSFNDGDEWMNAQLLFKKK 260
C+GGY QFS+N+G+EWM AQ+LFKKK
Sbjct: 337 CMGGYEQFSYNNGEEWMVAQMLFKKK 362
>ANY58195.1 TNMT [synthetic construct]
Length = 350
Score = 261 bits (668), Expect = 3e-83
Identities = 126/206 (61%), Positives = 148/206 (71%), Gaps = 37/206 (17%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNI 587
NCHVTGLTNSK Q NYI +Q EKL L NVDV+ ADVT++++DKTYDRILV+ +EHMKNI
Sbjct: 145 NCHVTGLTNSKAQANYIEQQAEKLELANVDVIFADVTKFDTDKTYDRILVVETIEHMKNI 204
Query: 586 QLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLY 407
QLFMKKLSTWMTEDS LFVDH+ HKTF+H FEA+DEDDWYSG+IF GC+T+LS+ +LLY
Sbjct: 205 QLFMKKLSTWMTEDSLLFVDHISHKTFNHNFEALDEDDWYSGFIFPKGCVTILSSSTLLY 264
Query: 406 FQDDVSVVDHWVXNG-------------------------------------VITHIRTL 338
FQDDVS +DHWV NG VITHIRT
Sbjct: 265 FQDDVSALDHWVVNGMHMARSVEAWRKKLDNTIEAAREILEPGLGSKEAVNQVITHIRTF 324
Query: 337 CLGGYVQFSFNDGDEWMNAQLLFKKK 260
C+GGY QFS+N+G+EWM Q+LFKKK
Sbjct: 325 CIGGYEQFSYNNGEEWMITQILFKKK 350
>C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT ACO90222.1 tetrahydroprotoberberine
N-methyltransferase [Eschscholzia californica]
Length = 350
Score = 261 bits (668), Expect = 3e-83
Identities = 126/206 (61%), Positives = 148/206 (71%), Gaps = 37/206 (17%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNI 587
NCHVTGLTNSK Q NYI +Q EKL L NVDV+ ADVT++++DKTYDRILV+ +EHMKNI
Sbjct: 145 NCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVTKFDTDKTYDRILVVETIEHMKNI 204
Query: 586 QLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLY 407
QLFMKKLSTWMTEDS LFVDH+ HKTF+H FEA+DEDDWYSG+IF GC+T+LS+ +LLY
Sbjct: 205 QLFMKKLSTWMTEDSLLFVDHISHKTFNHNFEALDEDDWYSGFIFPKGCVTILSSSTLLY 264
Query: 406 FQDDVSVVDHWVXNG-------------------------------------VITHIRTL 338
FQDDVS +DHWV NG VITHIRT
Sbjct: 265 FQDDVSALDHWVVNGMHMARSVEAWRKKLDETIEAAREILEPGLGSKEAVNQVITHIRTF 324
Query: 337 CLGGYVQFSFNDGDEWMNAQLLFKKK 260
C+GGY QFS+N+G+EWM Q+LFKKK
Sbjct: 325 CIGGYEQFSYNNGEEWMITQILFKKK 350
>ANY58201.1 putative N-methyltransferase [synthetic construct]
Length = 365
Score = 261 bits (668), Expect = 4e-83
Identities = 127/206 (61%), Positives = 149/206 (72%), Gaps = 37/206 (17%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNI 587
NC VTG+TNSK Q+NYI+ Q EKL L NVDV+LADVT+++SD+TYDRILVI +EHMKNI
Sbjct: 160 NCRVTGITNSKAQMNYIVMQAEKLQLSNVDVILADVTKFDSDQTYDRILVIETIEHMKNI 219
Query: 586 QLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLY 407
QLFMKKLSTW+ +DS LFVDH+CHKTFSH FE +DE+DWYSG+IF GC+T+ SA +LLY
Sbjct: 220 QLFMKKLSTWLKKDSLLFVDHICHKTFSHHFETLDEEDWYSGFIFPKGCVTLPSASALLY 279
Query: 406 FQDDVSVVDHWVXNG-------------------------------------VITHIRTL 338
FQDDVSVVDHWV NG VITHIRT
Sbjct: 280 FQDDVSVVDHWVVNGMHMARSVEEWGKKLDKNMEAAREILEPGLGSKEAVNQVITHIRTF 339
Query: 337 CLGGYVQFSFNDGDEWMNAQLLFKKK 260
C+GGY QFSFN+G+EWM AQ+LFKKK
Sbjct: 340 CIGGYEQFSFNNGEEWMVAQMLFKKK 365
>ANY58193.1 putative N-methyltransferase [synthetic construct]
Length = 360
Score = 259 bits (661), Expect = 4e-82
Identities = 126/206 (61%), Positives = 149/206 (72%), Gaps = 37/206 (17%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNI 587
NCHVTGLTNSK Q+NYI+ Q +KL L NVDV+ ADVT++++DKTYDRILVI A+EHMKNI
Sbjct: 155 NCHVTGLTNSKAQMNYILMQAKKLQLSNVDVISADVTKFDNDKTYDRILVIEAIEHMKNI 214
Query: 586 QLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLY 407
QLF+KKLSTWM +DS LFVDH+ HK F+H FEA+DE+DWYSG+IF GC+T+LS+ +LLY
Sbjct: 215 QLFLKKLSTWMKKDSLLFVDHISHKKFNHHFEALDEEDWYSGFIFPKGCVTILSSSTLLY 274
Query: 406 FQDDVSVVDHWVXNG-------------------------------------VITHIRTL 338
FQDDVSVVDHWV NG VITHIRT
Sbjct: 275 FQDDVSVVDHWVVNGMHMARSMEEWRKKLDKNMEAAREILEPGLGSKEAVNQVITHIRTF 334
Query: 337 CLGGYVQFSFNDGDEWMNAQLLFKKK 260
CLGGY QFSFN+G+EWM Q+LFKKK
Sbjct: 335 CLGGYEQFSFNNGEEWMITQMLFKKK 360
>ANY58191.1 putative N-methyltransferase [synthetic construct]
Length = 350
Score = 251 bits (640), Expect = 5e-79
Identities = 123/209 (58%), Positives = 150/209 (71%), Gaps = 40/209 (19%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYE--SDKTYDRILVIGAVEHMK 593
NCHVTGLTNS+ QVNYI Q++KL L NV+ +LADVT++E ++KT+DRI++I A+EHMK
Sbjct: 142 NCHVTGLTNSRAQVNYIEMQSKKLNLTNVEAILADVTKHEFKTEKTFDRIILIEAIEHMK 201
Query: 592 NIQLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSL 413
NI+LFMKKLSTWMTE+S LFVDH+CHKTF+H FEA+DE+DWYSGYIF GC+T+LS+ +L
Sbjct: 202 NIELFMKKLSTWMTEESLLFVDHICHKTFNHHFEAMDEEDWYSGYIFPKGCVTILSSSAL 261
Query: 412 LYFQDDVSVVDHWVXNG--------------------------------------VITHI 347
LYFQ DVSVVDHWV NG VITH
Sbjct: 262 LYFQSDVSVVDHWVVNGNHMARSVQEWLKKLDDNMETVRGILEPAMGGSKEAVDQVITHS 321
Query: 346 RTLCLGGYVQFSFNDGDEWMNAQLLFKKK 260
RT C+G Y QFSFN+G+EWM AQ+LFKKK
Sbjct: 322 RTFCMGAYEQFSFNNGEEWMVAQILFKKK 350
>AKN62838.1 (S)-tetrahydroprotoberberine-cis-N-methyltransferase, partial
[Papaver bracteatum]
Length = 283
Score = 244 bits (622), Expect = 3e-77
Identities = 109/135 (80%), Positives = 125/135 (92%)
Frame = -3
Query: 763 CHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNIQ 584
CHVTGLTNSK QVNY++KQ EKLGL NVD +LADVTQYESDKTYDR+L+I A+EHMKN+Q
Sbjct: 114 CHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQ 173
Query: 583 LFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLYF 404
LFMKKLSTWMTE+S LFVDH+CHKTF+H FEA+DEDDWYSG+IF PGC T+L+A+SLLYF
Sbjct: 174 LFMKKLSTWMTEESLLFVDHVCHKTFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYF 233
Query: 403 QDDVSVVDHWVXNGV 359
QDDVSVVDHWV NG+
Sbjct: 234 QDDVSVVDHWVVNGM 248
>ANY58192.1 putative N-methyltransferase [synthetic construct]
Length = 353
Score = 230 bits (587), Expect = 5e-71
Identities = 115/209 (55%), Positives = 145/209 (69%), Gaps = 40/209 (19%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQ--YESDKTYDRILVIGAVEHMK 593
NCHVTGLTNSK QV +I QT+KL L NV+V+LADVT+ ++++KT+DRI++I +EHMK
Sbjct: 145 NCHVTGLTNSKAQVKHIELQTKKLNLTNVEVILADVTKHVFKTEKTFDRIILIQVIEHMK 204
Query: 592 NIQLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSL 413
NI+LFM+KLSTWMTE+S LFVDH+CHKTF++ FE +DE+DWYSG+IF GC+T+LS+ SL
Sbjct: 205 NIELFMRKLSTWMTEESLLFVDHICHKTFNYHFEPMDENDWYSGHIFPEGCVTILSSSSL 264
Query: 412 LYFQDDVSVVDHWVXNG--------------------------------------VITHI 347
LYFQ DVSV+DHWV NG V+T
Sbjct: 265 LYFQKDVSVLDHWVVNGKHMARSAQEWLKSLDDNMEKVREILEPSMGGNKEAVDQVLTQS 324
Query: 346 RTLCLGGYVQFSFNDGDEWMNAQLLFKKK 260
R C+G Y QFS N+G+EWM AQLLFKKK
Sbjct: 325 RFFCMGAYEQFSNNNGEEWMFAQLLFKKK 353
>ANY58202.1 putative N-methyltransferase [synthetic construct]
Length = 333
Score = 226 bits (576), Expect = 1e-69
Identities = 103/136 (75%), Positives = 121/136 (88%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNI 587
NCHVTGLTNSK Q+NYI+ Q EKL L NVDV+ ADVT++++DKTYDRILVI A+EHMKNI
Sbjct: 155 NCHVTGLTNSKAQMNYILMQAEKLQLSNVDVISADVTKFDTDKTYDRILVIEAIEHMKNI 214
Query: 586 QLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLY 407
QLFMKKLSTWM EDS LFVDH+ HKTF+H FEA+DE+DWYSG+IF GC+T+LS+ +LLY
Sbjct: 215 QLFMKKLSTWMKEDSLLFVDHISHKTFNHHFEALDEEDWYSGFIFPKGCVTILSSSTLLY 274
Query: 406 FQDDVSVVDHWVXNGV 359
FQDDVSVVDHWV NG+
Sbjct: 275 FQDDVSVVDHWVVNGM 290
>ANY58196.1 putative N-methyltransferase [synthetic construct]
Length = 357
Score = 213 bits (543), Expect = 2e-64
Identities = 105/206 (50%), Positives = 133/206 (64%), Gaps = 37/206 (17%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNI 587
NCHVTG+TNS Q N I ++ L L NV+V+LADVTQY++ T+DRI++I AVEHMKNI
Sbjct: 152 NCHVTGITNSNAQKNIIEEECRNLKLSNVEVILADVTQYDTKDTFDRIILIEAVEHMKNI 211
Query: 586 QLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLY 407
LF+KK+S WM +D +F+D +CHKTF H FEAIDEDDWYSGYIF G +T+ +A +LLY
Sbjct: 212 DLFLKKISKWMKDDGLMFIDCICHKTFGHHFEAIDEDDWYSGYIFPKGSVTLQAASTLLY 271
Query: 406 FQDDVSVVDHWVXNG-------------------------------------VITHIRTL 338
FQ+DV+VVD W +G VITH RT
Sbjct: 272 FQEDVAVVDQWAVSGKHMARTVEEWLKKLDKNIDVAREILEPSLGSKEAVEKVITHSRTF 331
Query: 337 CLGGYVQFSFNDGDEWMNAQLLFKKK 260
C+G QFS+N+GDEWM + +LFKKK
Sbjct: 332 CIGTSEQFSYNNGDEWMISHVLFKKK 357
>AOR51553.1 putative N-methyltransferase [Papaver somniferum]
Length = 356
Score = 207 bits (528), Expect = 4e-62
Identities = 111/209 (53%), Positives = 134/209 (64%), Gaps = 40/209 (19%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVT--QYESDKTYDRILVIGAVEHMK 593
N HVTGLTNS EQ NYI+ Q EKL L NVDV+LADVT ++E++K +DRILV+ A+EHMK
Sbjct: 148 NSHVTGLTNSAEQRNYIMLQVEKLSLSNVDVILADVTKHEFENEKKFDRILVVEAIEHMK 207
Query: 592 NIQLFMKKLSTWMT-EDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADS 416
NIQLF+KK+S WM EDSFLFV HLCHK FS FEA+DEDDWY+ Y+ G +T LSA +
Sbjct: 208 NIQLFLKKISNWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASA 267
Query: 415 LLYFQDDVSVVDHWVXNG-------------------------------------VITHI 347
LLYFQDDVSVVD W+ +G V
Sbjct: 268 LLYFQDDVSVVDQWLLSGTHMARSQEEWFKRFKINLEAASKALTVGLGSEEAANQVNIQY 327
Query: 346 RTLCLGGYVQFSFNDGDEWMNAQLLFKKK 260
+T +G VQFSFN+G+EWM + LFKKK
Sbjct: 328 KTFFMGYVVQFSFNNGEEWMISHYLFKKK 356
>5KN4_A Chain A, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KN4_B Chain
B, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KOC_A
Chain A, Pavine N-methyltransferase In Complex With
S-adenosylmethionine Ph 7 5KOC_B Chain B, Pavine
N-methyltransferase In Complex With S-adenosylmethionine
Ph 7 5KOK_A Chain A, Pavine N-methyltransferase In
Complex With Tetrahydropapaverine And S-
Adenosylhomocysteine Ph 7.25 5KOK_B Chain B, Pavine
N-methyltransferase In Complex With Tetrahydropapaverine
And S- Adenosylhomocysteine Ph 7.25 5KPG_A Chain A,
Pavine N-methyltransferase In Complex With
S-adenosylhomocysteine Ph 7 5KPG_B Chain B, Pavine
N-methyltransferase In Complex With
S-adenosylhomocysteine Ph 7
Length = 397
Score = 182 bits (463), Expect = 6e-52
Identities = 88/205 (42%), Positives = 124/205 (60%), Gaps = 37/205 (18%)
Frame = -3
Query: 763 CHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNIQ 584
C VTG+TNS Q +I+ Q +KL L NV+++L DVT++E++ TYDRI + +EHMKN +
Sbjct: 193 CKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDVTKFETEITYDRIFAVALIEHMKNYE 252
Query: 583 LFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLYF 404
LF+KK+STW+ +D LFV+H CHK F++ +E +DEDDWY+ YIF G L + S+ LLYF
Sbjct: 253 LFLKKVSTWIAQDGLLFVEHHCHKVFAYQYEPLDEDDWYTEYIFPSGTLVMSSSSILLYF 312
Query: 403 QDDVSVVDHWVXNG-------------------------------------VITHIRTLC 335
Q+DVSVV+HW +G IT+ R C
Sbjct: 313 QEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEIFESFYGSKEKAMKFITYWRVFC 372
Query: 334 LGGYVQFSFNDGDEWMNAQLLFKKK 260
+ +S N+G+EWM +Q+LFKKK
Sbjct: 373 IAHSQMYSTNNGEEWMLSQVLFKKK 397
>C3SBW0.1 RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT ACO90251.1
pavine N-methyltransferase [Thalictrum flavum]
ANY58204.1 PNMT [synthetic construct]
Length = 356
Score = 179 bits (454), Expect = 5e-51
Identities = 87/205 (42%), Positives = 123/205 (60%), Gaps = 37/205 (18%)
Frame = -3
Query: 763 CHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNIQ 584
C VTG+TNS Q +I+ Q +KL L NV+++L DVT++E++ TYDRI + +EHMKN +
Sbjct: 152 CKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDVTKFETEITYDRIFAVALIEHMKNYE 211
Query: 583 LFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLYF 404
LF+KK+STW+ + LFV+H CHK F++ +E +DEDDWY+ YIF G L + S+ LLYF
Sbjct: 212 LFLKKVSTWIAQYGLLFVEHHCHKVFAYQYEPLDEDDWYTEYIFPSGTLVMSSSSILLYF 271
Query: 403 QDDVSVVDHWVXNG-------------------------------------VITHIRTLC 335
Q+DVSVV+HW +G IT+ R C
Sbjct: 272 QEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEIFESFYGSKEKAMKFITYWRVFC 331
Query: 334 LGGYVQFSFNDGDEWMNAQLLFKKK 260
+ +S N+G+EWM +Q+LFKKK
Sbjct: 332 IAHSQMYSTNNGEEWMLSQVLFKKK 356
>5KPC_A Chain A, Pavine N-methyltransferase H206a Mutant In Complex With S-
Adenosylmethionine Ph 6 5KPC_B Chain B, Pavine
N-methyltransferase H206a Mutant In Complex With S-
Adenosylmethionine Ph 6
Length = 397
Score = 179 bits (453), Expect = 2e-50
Identities = 87/205 (42%), Positives = 123/205 (60%), Gaps = 37/205 (18%)
Frame = -3
Query: 763 CHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNIQ 584
C VTG+TNS Q +I+ Q +KL L NV+++L DVT++E++ TYDRI + +E MKN +
Sbjct: 193 CKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDVTKFETEITYDRIFAVALIEAMKNYE 252
Query: 583 LFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLYF 404
LF+KK+STW+ +D LFV+H CHK F++ +E +DEDDWY+ YIF G L + S+ LLYF
Sbjct: 253 LFLKKVSTWIAQDGLLFVEHHCHKVFAYQYEPLDEDDWYTEYIFPSGTLVMSSSSILLYF 312
Query: 403 QDDVSVVDHWVXNG-------------------------------------VITHIRTLC 335
Q+DVSVV+HW +G IT+ R C
Sbjct: 313 QEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEIFESFYGSKEKAMKFITYWRVFC 372
Query: 334 LGGYVQFSFNDGDEWMNAQLLFKKK 260
+ +S N+G+EWM +Q+LFKKK
Sbjct: 373 IAHSQMYSTNNGEEWMLSQVLFKKK 397
>BAB71802.1 coclaurine N-methyltransferase [Coptis japonica]
Length = 358
Score = 175 bits (443), Expect = 2e-49
Identities = 84/206 (40%), Positives = 122/206 (59%), Gaps = 37/206 (17%)
Frame = -3
Query: 766 NCHVTGLTNSKEQVNYIIKQTEKLGLKNVDVLLADVTQYESDKTYDRILVIGAVEHMKNI 587
NC VT +TNS Q YI +++ + L NV+V LAD+T +E +TYDRILVI EHMKN
Sbjct: 153 NCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMAETYDRILVIELFEHMKNY 212
Query: 586 QLFMKKLSTWMTEDSFLFVDHLCHKTFSHLFEAIDEDDWYSGYIFSPGCLTVLSADSLLY 407
+L ++K+S W+++D LF++H+CHKTF++ +E +D+DDW++ Y+F G + + SA LY
Sbjct: 213 ELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVFPAGTMIIPSASFFLY 272
Query: 406 FQDDVSVVDHWVXNG-------------------------------------VITHIRTL 338
FQDDVSVV+HW +G +I + R
Sbjct: 273 FQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVKLINYWRGF 332
Query: 337 CLGGYVQFSFNDGDEWMNAQLLFKKK 260
CL G F +N+G+EWM + +LFKKK
Sbjct: 333 CLSGMEMFGYNNGEEWMASHVLFKKK 358