BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver32_contig00004044
         (1227 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

P93479.1 RecName: Full=Reticuline oxidase; AltName: Full=Berberi...   711   0.0  
AGL44333.1 FAD-dependent oxidoreductase, partial [Papaver somnif...   705   0.0  
ACJ76783.1 berberine bridge enzyme 1 [Argemone mexicana]              619   0.0  
ACJ76784.1 berberine bridge enzyme 2 [Argemone mexicana]              597   0.0  
3FW9_A Chain A, Structure Of Berberine Bridge Enzyme In Complex ...   580   0.0  
4EC3_A Chain A, Structure Of Berberine Bridge Enzyme, H174a Vari...   580   0.0  
3GSY_A Chain A, Structure Of Berberine Bridge Enzyme In Complex ...   580   0.0  
P30986.1 RecName: Full=Reticuline oxidase; AltName: Full=Berberi...   580   0.0  
3D2D_A Chain A, Structure Of Berberine Bridge Enzyme In Complex ...   580   0.0  
3FW8_A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant   576   0.0  
3FWA_A Chain A, Structure Of Berberine Bridge Enzyme, C166a Vari...   576   0.0  
3FW7_A Chain A, Structure Of Berberine Bridge Enzyme, H104a Variant   576   0.0  
4PZF_A Chain A, Berberine Bridge Enzyme G164a Variant, A Reticul...   577   0.0  
AAD17487.1 berberine bridge enzyme [Berberis stolonifera]             503   e-173
AAU20769.1 berberine bridge enzyme [Thalictrum flavum subsp. gla...   484   e-165
BAM44344.1 berberine bridge enzyme [Coptis japonica]                  478   e-163
KYP74605.1 Reticuline oxidase [Cajanus cajan]                         373   e-123
KHN05030.1 Reticuline oxidase [Glycine soja]                          372   e-123
XP_017414603.1 PREDICTED: reticuline oxidase-like [Vigna angular...   374   e-122
KHN30536.1 Reticuline oxidase [Glycine soja]                          370   e-122

>P93479.1 RecName: Full=Reticuline oxidase; AltName: Full=Berberine
            bridge-forming enzyme; Short=BBE; AltName:
            Full=Tetrahydroprotoberberine synthase; Flags: Precursor
            AAC61839.1 berberine bridge enzyme [Papaver somniferum]
          Length = 535

 Score =  711 bits (1835), Expect = 0.0
 Identities = 354/408 (86%), Positives = 368/408 (90%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYTADTPFVIVDMMNL+ ISID  SETAWVESGATLGELYYAIAQST+TLGF
Sbjct: 106  GGHSYEGLSYTADTPFVIVDMMNLNRISIDVLSETAWVESGATLGELYYAIAQSTDTLGF 165

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGWCPTV               RKYGLAADNVVDAILIDS GAILDREKMG+DVFWAIR
Sbjct: 166  TAGWCPTVGSGGHISGGGFGMMSRKYGLAADNVVDAILIDSNGAILDREKMGDDVFWAIR 225

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEKLTVFRVTKNV IE ASSLLHKWQYVADELD DFTVSV
Sbjct: 226  GGGGGVWGAIYAWKIKLLPVPEKLTVFRVTKNVGIEDASSLLHKWQYVADELDEDFTVSV 285

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGGVNGN+AWLMFLGLHLGRKDAAK+II EKFPELGLV+KEFQEMSWG SMAFL+GL+TI
Sbjct: 286  LGGVNGNDAWLMFLGLHLGRKDAAKTIIDEKFPELGLVDKEFQEMSWGESMAFLSGLDTI 345

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            SELNNRFLKFD+RAFKTKVDFTK +VPL+VFR ALEMLSEQPGGFIALNGFGGKMSEIST
Sbjct: 346  SELNNRFLKFDERAFKTKVDFTKVSVPLNVFRHALEMLSEQPGGFIALNGFGGKMSEIST 405

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHRKGTKLMFEYIIAWNQDEE+KIGEFSEWL KFYDYLEPFVSKEPRVGYVNHID
Sbjct: 406  DFTPFPHRKGTKLMFEYIIAWNQDEESKIGEFSEWLAKFYDYLEPFVSKEPRVGYVNHID 465

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LDIGGIDW+NKSST+NAVEIARNWGERYFSSNYERLVKAKTLIDPNNV
Sbjct: 466  LDIGGIDWRNKSSTTNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 513


>AGL44333.1 FAD-dependent oxidoreductase, partial [Papaver somniferum]
          Length = 535

 Score =  705 bits (1820), Expect = 0.0
 Identities = 353/408 (86%), Positives = 366/408 (89%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYTADTPFVIVDMMNL+ ISID ESETAWVESGATLGELYYAIAQSTETLGF
Sbjct: 106  GGHSYEGLSYTADTPFVIVDMMNLNRISIDVESETAWVESGATLGELYYAIAQSTETLGF 165

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGWCPTV               RKYGLAADNVVDAILIDS G+ILDREKMGEDVFWAIR
Sbjct: 166  TAGWCPTVGSGGHISGGGFGMMSRKYGLAADNVVDAILIDSTGSILDREKMGEDVFWAIR 225

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEKLTVFRVTKNV IE ASSLLHKWQYVADELD DFTVSV
Sbjct: 226  GGGGGVWGAVYAWKIKLLPVPEKLTVFRVTKNVGIEGASSLLHKWQYVADELDEDFTVSV 285

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGGVNGN+AWLMFLGL+LGR+DAAK+II EKFPELG VE+EFQEMSW AS AFL+GLET+
Sbjct: 286  LGGVNGNDAWLMFLGLYLGREDAAKTIIDEKFPELGSVEEEFQEMSWVASTAFLSGLETV 345

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            SELNNRFLKFD+RAFKTKVDFTKE VPL+VFRRALEMLSEQPGGFIALNGFGGKMSEIST
Sbjct: 346  SELNNRFLKFDERAFKTKVDFTKEPVPLNVFRRALEMLSEQPGGFIALNGFGGKMSEIST 405

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHRKGTKLMFEYIIAWNQDEETKI EFSEWL KFYDYLEPFVSK PRVGYVNHID
Sbjct: 406  DFTPFPHRKGTKLMFEYIIAWNQDEETKIEEFSEWLTKFYDYLEPFVSKGPRVGYVNHID 465

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            L+IGGIDW+NKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV
Sbjct: 466  LEIGGIDWRNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 513


>ACJ76783.1 berberine bridge enzyme 1 [Argemone mexicana]
          Length = 554

 Score =  619 bits (1595), Expect = 0.0
 Identities = 295/408 (72%), Positives = 341/408 (83%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYTADTPFV++D+MNL+ ISID +SETAWVESGATLGELYYAI + T++LGF
Sbjct: 114  GGHSYEGLSYTADTPFVLIDLMNLNRISIDIDSETAWVESGATLGELYYAITELTDSLGF 173

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGWCPTV               RKYGLAADNV D ILIDSKGAILDR+ MGEDVFWA+R
Sbjct: 174  TAGWCPTVGSGGHISGGGFGMMSRKYGLAADNVEDVILIDSKGAILDRKLMGEDVFWAVR 233

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVP+K+TVFRVTKNV IE AS L+HKWQYVADELD+DFTVS+
Sbjct: 234  GGGGGVWGAIYAWKIKLLPVPKKVTVFRVTKNVNIEEASFLIHKWQYVADELDDDFTVSI 293

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG NGNE W++FLGLHLG K  AKSII +KFPELGL+E+EF EM+WG S A+L+GL+T+
Sbjct: 294  LGGANGNEVWVIFLGLHLGCKTVAKSIIDKKFPELGLIEEEFLEMNWGESFAYLSGLKTV 353

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
             ELNNRFLKFDDRAFKTKVDFTKE +PL+     LE+LS++P GFIALNGFGGKMS+IS 
Sbjct: 354  KELNNRFLKFDDRAFKTKVDFTKETLPLEAIDGLLEILSKEPRGFIALNGFGGKMSKISN 413

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHRKGTKLM EYI+AW++DEE+K  EF +WL   YDY+E FVSK PRVGYVNHID
Sbjct: 414  DFTPFPHRKGTKLMVEYIVAWSKDEESKSDEFFDWLRNIYDYMEMFVSKNPRVGYVNHID 473

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW +K+S++NA+EIARNWGE+YF SNYERL++AKTLIDPNNV
Sbjct: 474  LDLGGIDWSDKNSSNNAIEIARNWGEKYFLSNYERLIRAKTLIDPNNV 521


>ACJ76784.1 berberine bridge enzyme 2 [Argemone mexicana]
          Length = 536

 Score =  597 bits (1540), Expect = 0.0
 Identities = 283/408 (69%), Positives = 334/408 (81%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYTADTPFV++D+MNL+ ISID +SETAWVESGAT+GELYYAI++ST++ GF
Sbjct: 109  GGHSYEGLSYTADTPFVLIDLMNLNRISIDMDSETAWVESGATVGELYYAISESTDSFGF 168

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGWCPTV               RKYGLAADNV D ILIDS GAILDR+ MGEDVFWA+R
Sbjct: 169  TAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVEDVILIDSNGAILDRKLMGEDVFWAVR 228

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVP+K+TVFRV KNV IE AS L+HKWQYVADELD+DFTV++
Sbjct: 229  GGGGGVWGAIYAWKIKLLPVPKKVTVFRVMKNVNIEEASFLIHKWQYVADELDDDFTVTI 288

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG NGN AWL+FLGLHLG K  AKSI+ + FPELGL+E+EF EM+WG S A+L+GL+T+
Sbjct: 289  LGGANGNGAWLVFLGLHLGCKTVAKSIMDKMFPELGLIEEEFLEMNWGESFAYLSGLKTV 348

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
             ELNNRFLK DD+AFKTKVDFTKE +PL V    LE+LS++P GFI LN  GGKMS+IS 
Sbjct: 349  KELNNRFLKLDDKAFKTKVDFTKETLPLKVINGVLEILSKEPRGFILLNSLGGKMSKISN 408

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHR GTKLM EYI++W++DEE+K  EF +WL   YDY+E FVSK PRVGYVN+ID
Sbjct: 409  DFTPFPHRNGTKLMVEYIVSWSKDEESKSDEFFDWLRNIYDYMEEFVSKNPRVGYVNNID 468

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW NK+S++NA+EIARNWGE+YF SNYERL++AKTLIDPNN+
Sbjct: 469  LDLGGIDWSNKNSSNNAIEIARNWGEKYFLSNYERLIRAKTLIDPNNI 516


>3FW9_A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
            (S)-Scoulerine
          Length = 495

 Score =  580 bits (1494), Expect = 0.0
 Identities = 276/408 (67%), Positives = 327/408 (80%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGF
Sbjct: 77   GGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGF 136

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGWCPTV               RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR
Sbjct: 137  TAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIR 196

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SV
Sbjct: 197  GGGGGVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSV 256

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG +  + WL  LG H G K  AKS     FPELGLVE+++ EMSWG S A+LAGLET+
Sbjct: 257  LGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETV 316

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            S+LNNRFLKFD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+
Sbjct: 317  SQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISS 376

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHR GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHID
Sbjct: 377  DFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHID 436

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNV
Sbjct: 437  LDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNV 484


>4EC3_A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In
            Complex With (S)-Reticuline
          Length = 519

 Score =  580 bits (1494), Expect = 0.0
 Identities = 276/408 (67%), Positives = 327/408 (80%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGF
Sbjct: 83   GGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGF 142

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGWCPTV               RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR
Sbjct: 143  TAGWCPTVGTGGAISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIR 202

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SV
Sbjct: 203  GGGGGVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSV 262

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG +  + WL  LG H G K  AKS     FPELGLVE+++ EMSWG S A+LAGLET+
Sbjct: 263  LGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETV 322

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            S+LNNRFLKFD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+
Sbjct: 323  SQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISS 382

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHR GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHID
Sbjct: 383  DFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHID 442

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNV
Sbjct: 443  LDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNV 490


>3GSY_A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
            Dehydroscoulerine
          Length = 519

 Score =  580 bits (1494), Expect = 0.0
 Identities = 276/408 (67%), Positives = 327/408 (80%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGF
Sbjct: 83   GGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGF 142

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGWCPTV               RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR
Sbjct: 143  TAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIR 202

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SV
Sbjct: 203  GGGGGVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSV 262

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG +  + WL  LG H G K  AKS     FPELGLVE+++ EMSWG S A+LAGLET+
Sbjct: 263  LGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETV 322

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            S+LNNRFLKFD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+
Sbjct: 323  SQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISS 382

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHR GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHID
Sbjct: 383  DFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHID 442

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNV
Sbjct: 443  LDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNV 490


>P30986.1 RecName: Full=Reticuline oxidase; AltName: Full=Berberine
            bridge-forming enzyme; Short=BBE; AltName:
            Full=Tetrahydroprotoberberine synthase; Flags: Precursor
            AAB20352.1 (S)-reticuline:oxygen oxidoreductase
            (methylene-bridge-forming) [Eschscholzia californica]
            AAC39358.1 berberine bridge enzyme [Eschscholzia
            californica]
          Length = 538

 Score =  580 bits (1494), Expect = 0.0
 Identities = 276/408 (67%), Positives = 327/408 (80%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGF
Sbjct: 102  GGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGF 161

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGWCPTV               RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR
Sbjct: 162  TAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIR 221

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SV
Sbjct: 222  GGGGGVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSV 281

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG +  + WL  LG H G K  AKS     FPELGLVE+++ EMSWG S A+LAGLET+
Sbjct: 282  LGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETV 341

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            S+LNNRFLKFD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+
Sbjct: 342  SQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISS 401

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHR GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHID
Sbjct: 402  DFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHID 461

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNV
Sbjct: 462  LDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNV 509


>3D2D_A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
            (S)-Reticuline 3D2H_A Chain A, Structure Of Berberine
            Bridge Enzyme From Eschscholzia Californica, Monoclinic
            Crystal Form 3D2J_A Chain A, Structure Of Berberine
            Bridge Enzyme From Eschscholzia Californica, Tetragonal
            Crystal Form
          Length = 538

 Score =  580 bits (1494), Expect = 0.0
 Identities = 276/408 (67%), Positives = 327/408 (80%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGF
Sbjct: 102  GGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGF 161

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGWCPTV               RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR
Sbjct: 162  TAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIR 221

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SV
Sbjct: 222  GGGGGVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSV 281

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG +  + WL  LG H G K  AKS     FPELGLVE+++ EMSWG S A+LAGLET+
Sbjct: 282  LGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETV 341

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            S+LNNRFLKFD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+
Sbjct: 342  SQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISS 401

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHR GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHID
Sbjct: 402  DFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHID 461

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNV
Sbjct: 462  LDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNV 509


>3FW8_A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant
          Length = 495

 Score =  576 bits (1485), Expect = 0.0
 Identities = 275/408 (67%), Positives = 326/408 (79%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGF
Sbjct: 77   GGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGF 136

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGW PTV               RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR
Sbjct: 137  TAGWAPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIR 196

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SV
Sbjct: 197  GGGGGVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSV 256

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG +  + WL  LG H G K  AKS     FPELGLVE+++ EMSWG S A+LAGLET+
Sbjct: 257  LGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETV 316

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            S+LNNRFLKFD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+
Sbjct: 317  SQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISS 376

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHR GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHID
Sbjct: 377  DFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHID 436

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNV
Sbjct: 437  LDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNV 484


>3FWA_A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant In
            Complex With (S)-Reticuline
          Length = 497

 Score =  576 bits (1485), Expect = 0.0
 Identities = 275/408 (67%), Positives = 326/408 (79%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGF
Sbjct: 77   GGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGF 136

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGW PTV               RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR
Sbjct: 137  TAGWAPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIR 196

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SV
Sbjct: 197  GGGGGVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSV 256

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG +  + WL  LG H G K  AKS     FPELGLVE+++ EMSWG S A+LAGLET+
Sbjct: 257  LGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETV 316

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            S+LNNRFLKFD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+
Sbjct: 317  SQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISS 376

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHR GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHID
Sbjct: 377  DFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHID 436

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNV
Sbjct: 437  LDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNV 484


>3FW7_A Chain A, Structure Of Berberine Bridge Enzyme, H104a Variant
          Length = 498

 Score =  576 bits (1484), Expect = 0.0
 Identities = 275/408 (67%), Positives = 326/408 (79%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GG SYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGF
Sbjct: 80   GGASYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGF 139

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TAGWCPTV               RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR
Sbjct: 140  TAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIR 199

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SV
Sbjct: 200  GGGGGVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSV 259

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG +  + WL  LG H G K  AKS     FPELGLVE+++ EMSWG S A+LAGLET+
Sbjct: 260  LGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETV 319

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            S+LNNRFLKFD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+
Sbjct: 320  SQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISS 379

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHR GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHID
Sbjct: 380  DFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHID 439

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNV
Sbjct: 440  LDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNV 487


>4PZF_A Chain A, Berberine Bridge Enzyme G164a Variant, A Reticuline
            Dehydrogenase 4PZF_B Chain B, Berberine Bridge Enzyme
            G164a Variant, A Reticuline Dehydrogenase 4PZF_C Chain C,
            Berberine Bridge Enzyme G164a Variant, A Reticuline
            Dehydrogenase 4PZF_D Chain D, Berberine Bridge Enzyme
            G164a Variant, A Reticuline Dehydrogenase
          Length = 538

 Score =  577 bits (1488), Expect = 0.0
 Identities = 275/408 (67%), Positives = 326/408 (79%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGF
Sbjct: 102  GGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGF 161

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            TA WCPTV               RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR
Sbjct: 162  TAAWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIR 221

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         KIKLLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SV
Sbjct: 222  GGGGGVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSV 281

Query: 542  LGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLETI 721
            LGG +  + WL  LG H G K  AKS     FPELGLVE+++ EMSWG S A+LAGLET+
Sbjct: 282  LGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETV 341

Query: 722  SELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIST 901
            S+LNNRFLKFD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+
Sbjct: 342  SQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISS 401

Query: 902  DFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHID 1081
            DFTPFPHR GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHID
Sbjct: 402  DFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHID 461

Query: 1082 LDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            LD+GGIDW NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNV
Sbjct: 462  LDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNV 509


>AAD17487.1 berberine bridge enzyme [Berberis stolonifera]
          Length = 536

 Score =  503 bits (1296), Expect = e-173
 Identities = 240/409 (58%), Positives = 307/409 (75%), Gaps = 1/409 (0%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLSY ADTPFV++D+MNL+ ISID ES+TAWVESGATLGE+Y AI+++++TLGF
Sbjct: 98   GGHSYEGLSYVADTPFVVIDLMNLNRISIDLESKTAWVESGATLGEIYCAISEASDTLGF 157

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            + G+CPTV               RKYGLAADNV+DA+++D+ GA+LDR  MGEDVFWAIR
Sbjct: 158  SGGYCPTVGSGGHISGGGFGMMSRKYGLAADNVIDALIVDANGAVLDRSSMGEDVFWAIR 217

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNV-KIEAASSLLHKWQYVADELDNDFTVS 538
                         K++LLPVP+++TVF++ KN   IE AS +LHKWQ VA  L++DFT+S
Sbjct: 218  GGGGGVWGAIYAWKLQLLPVPKQVTVFKLMKNFDNIEEASKMLHKWQVVAPALEDDFTLS 277

Query: 539  VLGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLET 718
            VL G + N  W  FLGL+LG K+ A S + + FPEL LV ++ +EMSW  S A LAGL +
Sbjct: 278  VLAGADTNGIWFSFLGLYLGPKELAISSVDQNFPELNLVMEDCKEMSWVESFAHLAGLNS 337

Query: 719  ISELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIS 898
            + E+NNRFLK+DDRAFKTKVDF KE +PL+  + AL ML+++  GF+A NG GG MS IS
Sbjct: 338  VEEMNNRFLKYDDRAFKTKVDFVKEPIPLEGIKGALTMLTKELRGFMAFNGQGGLMSRIS 397

Query: 899  TDFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHI 1078
            +D TPFPHRKGT +M EYI+AW++DE+ K  EF  WL  FY+Y+  F+  +PR+ YVNH+
Sbjct: 398  SDSTPFPHRKGTLMMMEYIVAWDRDEDAKSYEFIGWLHGFYNYMGQFLPSDPRIAYVNHV 457

Query: 1079 DLDIGGIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            DLD+G +DW N +  SNA+EIAR WGE+YF SNYERLV+AKTLIDP NV
Sbjct: 458  DLDLGRLDWTNSTIASNAIEIARTWGEKYFLSNYERLVRAKTLIDPKNV 506


>AAU20769.1 berberine bridge enzyme [Thalictrum flavum subsp. glaucum]
          Length = 535

 Score =  484 bits (1245), Expect = e-165
 Identities = 232/410 (56%), Positives = 307/410 (74%), Gaps = 2/410 (0%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLS+ AD PFVI+D+MNL+GISID +++TAWVESGATLGE+Y+AI +S+ T+ F
Sbjct: 99   GGHSYEGLSHIADNPFVIIDLMNLNGISIDLDTQTAWVESGATLGEIYHAIGKSSGTMAF 158

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            +AG+CPT                RKYGLAADNVVDA+L+D+ G +LDRE MGEDVFWAIR
Sbjct: 159  SAGYCPTGGSGGHIAPGGFGMMSRKYGLAADNVVDALLVDANGVVLDRESMGEDVFWAIR 218

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTVSV 541
                         K++L+PVP+ +T+FR+ K+ ++E AS LLHKWQ VA +L++DF+++V
Sbjct: 219  GGGGGVWGAVYAWKLQLVPVPKNVTIFRLMKHSEVEDASKLLHKWQLVAPKLEDDFSLAV 278

Query: 542  LGGVNGNEA-WLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLET 718
            L G N + + WL FLGL+LG K+ A S + +KFPEL L+ ++  EMSW  + A LAGL++
Sbjct: 279  LAGTNKDSSIWLTFLGLYLGPKELASSSMHKKFPELNLLLEDCMEMSWVEATAELAGLKS 338

Query: 719  ISELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIS 898
            +SEL +RFL++DDRAFKTKVDF KEA+PL+  + ALE+L ++  GF+ +NG GG M  IS
Sbjct: 339  VSELKDRFLRYDDRAFKTKVDFPKEAIPLEGIQGALEILKKEQRGFMVMNGQGGMMDRIS 398

Query: 899  TDFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHI 1078
            TD +PFPHR GT  M EYI+AW++ E+    EF  WL + +DY+  FVS  PRVGYVNH+
Sbjct: 399  TDASPFPHRSGTLSMVEYIVAWDKHEDLHSNEFIHWLHQLFDYMGKFVSNNPRVGYVNHV 458

Query: 1079 DLDIGGIDWKNKS-STSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            DLD+G IDW NK+ S+  A+EIAR WGE+YF SNY+RLV+AKT+IDP NV
Sbjct: 459  DLDLGRIDWVNKTISSGRAIEIARTWGEKYFMSNYDRLVRAKTMIDPKNV 508


>BAM44344.1 berberine bridge enzyme [Coptis japonica]
          Length = 533

 Score =  478 bits (1231), Expect = e-163
 Identities = 234/410 (57%), Positives = 297/410 (72%), Gaps = 2/410 (0%)
 Frame = +2

Query: 2    GGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGF 181
            GGHSYEGLS+TAD+PFVI+DMMNL+G+SID +++TAW ESGATLGE+Y+AI  S++ LGF
Sbjct: 102  GGHSYEGLSHTADSPFVIIDMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSSDVLGF 161

Query: 182  TAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIR 361
            +AG+CPTV               RKYGLAADNVVDAILI + GA+ DR+ MGEDVFWAIR
Sbjct: 162  SAGYCPTVGSGGHISGGGFGMMSRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIR 221

Query: 362  XXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNV-KIEAASSLLHKWQYVADELDNDFTVS 538
                         K++LLPVP+ +TVF++TK+  +I+ AS LL+KWQ VA  LD+DF+++
Sbjct: 222  GGGGGVWGVVYAWKLQLLPVPKHVTVFKLTKHTSEIDEASKLLYKWQLVAPNLDDDFSLA 281

Query: 539  VLGGVNGNEAWLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAFLAGLET 718
            VL G   +  WL FLGL+LG K+ A S + +KFPEL L+ +E +E+SW  + A LAGL+ 
Sbjct: 282  VLNGAEKDGFWLTFLGLYLGPKEVAVSSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKE 341

Query: 719  ISELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEIS 898
            + ELNNRFLK+DDRAFKTKVDF +  +PL+    AL++L ++  GF+ +NG GG M  IS
Sbjct: 342  VDELNNRFLKYDDRAFKTKVDFAEVPIPLEGINGALQILKKEQRGFMVMNGQGGMMGRIS 401

Query: 899  TDFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHI 1078
             D  PFPHR G   M EYI+AW+ DE+    E+  WL +FYDY+  FV   PRVGYVNH+
Sbjct: 402  RDSIPFPHRSGMLSMIEYIVAWDMDEDFNSHEYINWLHQFYDYMGQFVGNNPRVGYVNHV 461

Query: 1079 DLDIGGIDWKNKS-STSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            D D G IDW N S S S A+EIAR WGE+YF SNY+RLV AKTLIDPNNV
Sbjct: 462  DFDFGTIDWTNSSISASKAIEIARTWGEKYFLSNYDRLVGAKTLIDPNNV 511


>KYP74605.1 Reticuline oxidase [Cajanus cajan]
          Length = 475

 Score =  373 bits (958), Expect = e-123
 Identities = 197/419 (47%), Positives = 269/419 (64%), Gaps = 11/419 (2%)
 Frame = +2

Query: 2    GGHSYEGLSYTAD--TPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETL 175
            GGHSYEG SY +D  TPFVI+DMMNL+ + ++ ESETAWVE GATLGE YYAI+Q++   
Sbjct: 36   GGHSYEGTSYVSDDGTPFVIIDMMNLNHVWVEMESETAWVEGGATLGETYYAISQASNEH 95

Query: 176  GFTAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWA 355
            GF+AG CPTV               RKYGLAADNVVDA+L+D+ G + DRE MGEDVFWA
Sbjct: 96   GFSAGSCPTVGVGGHIGGGGFGLLSRKYGLAADNVVDALLVDADGKLFDRESMGEDVFWA 155

Query: 356  IRXXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTV 535
            IR             KI++L VP+ +T F V++       + L+HKWQYVA  L+ DF +
Sbjct: 156  IRGGGGGLWGIVYAWKIQVLKVPQIVTSFTVSRTSTKSHVAMLVHKWQYVAPNLEGDFYL 215

Query: 536  SVLGGVNGNEA-----WLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAF 700
            S   G    +A        F G +LG   +A SI+ + FPELGLVE+E  EMSW  S+ F
Sbjct: 216  SCFVGAGLPQAKTTGLSTTFNGFYLGPMASAISILNQAFPELGLVEEECIEMSWIQSVVF 275

Query: 701  LAGLE---TISELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNG 871
             +GL     +S+L NR+L+ + + FK K D+ K+ VPL     ALE+L ++P G++ L+ 
Sbjct: 276  FSGLSDGALVSDLKNRYLQ-EKQYFKAKSDYVKQHVPLVGIETALEILEKEPKGYVILDP 334

Query: 872  FGGKMSEISTDFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKE 1051
            +GG M  IS++   FPHR+G     +Y+I W + +  K  ++ +W+ +FY  + PFVS  
Sbjct: 335  YGGIMHNISSESIAFPHRRGNLFTIQYLIYWKEADNDKSKDYVDWIREFYAAMTPFVSWG 394

Query: 1052 PRVGYVNHIDLDIGGI-DWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            PR  Y+N++D D+G + +  N ++  +AVE AR WGE+YF SNY+RLV+AKTLIDPNNV
Sbjct: 395  PRAAYINYMDFDLGVMEEISNGANEKDAVENARVWGEKYFLSNYDRLVRAKTLIDPNNV 453


>KHN05030.1 Reticuline oxidase [Glycine soja]
          Length = 447

 Score =  372 bits (955), Expect = e-123
 Identities = 194/419 (46%), Positives = 268/419 (63%), Gaps = 11/419 (2%)
 Frame = +2

Query: 2    GGHSYEGLSYTAD--TPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETL 175
            GGHSYEG SY AD  TPFVI+DMMNL+ + +D E+ETAWVE GATLGE YYAI+Q +   
Sbjct: 8    GGHSYEGTSYVADDGTPFVIIDMMNLNHVWVDMETETAWVEGGATLGETYYAISQESNEH 67

Query: 176  GFTAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWA 355
            GF+ G CPTV               RKYGLAADNVVDA+L+D+ G + DRE MGEDVFWA
Sbjct: 68   GFSGGSCPTVGVGGHIGGGGFGLLSRKYGLAADNVVDALLVDANGKLFDRETMGEDVFWA 127

Query: 356  IRXXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTV 535
            IR             KI++L VP+ +T F V++       ++L+HKWQYVA  L++DF +
Sbjct: 128  IRGGGGGLWGIIYAWKIQVLKVPQVVTSFTVSRTGTKSHVANLVHKWQYVAPNLEDDFYL 187

Query: 536  SVLGGVNGNEA-----WLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAF 700
            S L G    +A        F G +LG + +A SI+ + FPEL + E+E  EMSW  S+ F
Sbjct: 188  SCLVGAGLPQAKTTGLSTTFNGFYLGPRASATSILNQAFPELSIAEEECIEMSWIQSIVF 247

Query: 701  LAGLE---TISELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNG 871
             +GL    ++S+L NR+L+ +   FK K D+ K+ VPL     AL++L ++P G++ L+ 
Sbjct: 248  FSGLSDGASVSDLKNRYLQ-EKEYFKAKSDYVKKNVPLVGIETALDILEKEPKGYVVLDP 306

Query: 872  FGGKMSEISTDFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKE 1051
            +GG M  IS++   FPHR+G     +Y+I W + +  K  ++ +W+  FY  + PFVS  
Sbjct: 307  YGGMMHNISSESIAFPHRRGNLFTIQYLIYWKEADNDKSSDYVDWIRGFYAAMTPFVSWG 366

Query: 1052 PRVGYVNHIDLDIGGIDW-KNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            PR  Y+N++D D+G ++   N ++  +AVE AR WGE+YF SNY+RLV+AKTLIDPNNV
Sbjct: 367  PRAAYINYMDFDLGVMEGIGNGANMKDAVEHARVWGEKYFLSNYDRLVRAKTLIDPNNV 425


>XP_017414603.1 PREDICTED: reticuline oxidase-like [Vigna angularis] KOM35607.1
            hypothetical protein LR48_Vigan02g175700 [Vigna
            angularis] BAT94832.1 hypothetical protein VIGAN_08147600
            [Vigna angularis var. angularis]
          Length = 546

 Score =  374 bits (961), Expect = e-122
 Identities = 195/419 (46%), Positives = 266/419 (63%), Gaps = 11/419 (2%)
 Frame = +2

Query: 2    GGHSYEGLSYTAD--TPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETL 175
            GGHSYEG SY AD  TPFVI+DMMNL+ + +D E+ETAWVE GATLGE YYAI++++   
Sbjct: 111  GGHSYEGTSYVADEGTPFVIIDMMNLNHVWVDMETETAWVEGGATLGETYYAISEASNEH 170

Query: 176  GFTAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWA 355
            GF+AG CPTV               RKYGLAADNVVDA+L+D+   +LDRE MGEDVFWA
Sbjct: 171  GFSAGSCPTVGVGGHIGGGGFGMLSRKYGLAADNVVDALLVDADQNLLDRETMGEDVFWA 230

Query: 356  IRXXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTV 535
            IR             KI+LL VP+ +T F V++       ++L+HKWQYVA  L++D+ +
Sbjct: 231  IRGGGGGLWGIIYAWKIQLLKVPQVVTSFTVSRTGTKSDVANLVHKWQYVAPNLEDDYYL 290

Query: 536  SVLGGVNGNEAWLMFL-----GLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAF 700
            S   G    +A    L     G +LG + +A SI+ E FPELG+ E+E  EMSW  S  F
Sbjct: 291  SCFVGAGLPQAKTKGLSVTLNGFYLGHRSSAVSILNETFPELGVTEEECIEMSWIESTVF 350

Query: 701  LAGLE---TISELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNG 871
             +GL    ++S+L NR+L+ +   FK K D+ ++ VPL     AL++L ++P G++ L+ 
Sbjct: 351  FSGLSDGASVSDLKNRYLQ-EKEYFKAKSDYVRKHVPLVGIETALDILEKEPKGYVILDA 409

Query: 872  FGGKMSEISTDFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKE 1051
            +GGKM  IS D   FPHR+G     +Y+I W + E  K  ++ +W+  FYD + PFVS  
Sbjct: 410  YGGKMHNISNDAIAFPHRRGNLFTIQYLIYWKEGENEKSSDYVDWIRGFYDAMTPFVSWG 469

Query: 1052 PRVGYVNHIDLDIG-GIDWKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            PR  Y+N++D D+G      N  +  +  E AR+WGE+YF SNY+RLV+AKT+IDPNNV
Sbjct: 470  PRAAYINYMDFDLGVRKRISNCGNVKDGAESARDWGEKYFLSNYDRLVRAKTMIDPNNV 528


>KHN30536.1 Reticuline oxidase [Glycine soja]
          Length = 447

 Score =  370 bits (949), Expect = e-122
 Identities = 195/419 (46%), Positives = 268/419 (63%), Gaps = 11/419 (2%)
 Frame = +2

Query: 2    GGHSYEGLSYTAD--TPFVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETL 175
            GGHSYEG SY AD  TPFVI+DMMNL+ + +D E+ETAWVE GATLGE YYAI+Q++   
Sbjct: 8    GGHSYEGTSYVADDGTPFVIIDMMNLNHVWVDMETETAWVEGGATLGETYYAISQASNEH 67

Query: 176  GFTAGWCPTVXXXXXXXXXXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWA 355
            GF+ G CPTV               RKYGLAADNVVDA+L+++ G + DRE MGEDVFWA
Sbjct: 68   GFSGGSCPTVGVGGHIGGGGFGILSRKYGLAADNVVDALLVNADGKLFDRETMGEDVFWA 127

Query: 356  IRXXXXXXXXXXXXXKIKLLPVPEKLTVFRVTKNVKIEAASSLLHKWQYVADELDNDFTV 535
            IR             KIK+L +P+ +T F V++       ++L+HKWQ VA  L++DF +
Sbjct: 128  IRGGGGGLWGIIYAWKIKVLKLPQVVTSFTVSRTGTKRHVANLVHKWQNVAPNLEDDFYL 187

Query: 536  SVLGGVNGNEA-----WLMFLGLHLGRKDAAKSIIGEKFPELGLVEKEFQEMSWGASMAF 700
            S   G    +A        F G +LG +  A SI+   FPELG+VE+E  EMSW  S  F
Sbjct: 188  SCFVGAGLPQAKTKGLSTTFNGFYLGPRAGAISILDHAFPELGIVEEECIEMSWIQSTVF 247

Query: 701  LAGLE---TISELNNRFLKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNG 871
             +GL    ++S+LNNR+L+ + + FK K D+ K+ VPL     AL++L ++P G++ L+ 
Sbjct: 248  FSGLSDGASVSDLNNRYLQ-EKQYFKAKSDYVKKHVPLVGIETALDILEKEPKGYVILDP 306

Query: 872  FGGKMSEISTDFTPFPHRKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKE 1051
            +GGKM  IS++   FPHR+G     +Y+I W + +  K  ++ +W+  FY  + PFVS  
Sbjct: 307  YGGKMHNISSESIAFPHRRGNLFTIQYLIYWKEADNDKNSDYVDWIRGFYAAMTPFVSWG 366

Query: 1052 PRVGYVNHIDLDIGGID-WKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNV 1225
            PR  YVN++D D+G ++   N ++  + VE AR WGE+YF SNY+RLV+AKTLIDPNNV
Sbjct: 367  PRAAYVNYMDFDLGVMERISNGANMKDVVEHARVWGEKYFLSNYDRLVRAKTLIDPNNV 425