BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver31_contig00019401
         (1437 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004142116.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   632   e-178
ref|XP_010102337.1| tRNA-specific 2-thiouridylase mnmA [Morus no...   621   e-175
ref|XP_008449720.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   619   e-174
ref|XP_002282827.2| PREDICTED: mitochondrial tRNA-specific 2-thi...   619   e-174
ref|XP_010257320.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   615   e-173
ref|XP_008238179.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   609   e-171
ref|XP_004507093.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   609   e-171
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   606   e-170
ref|XP_009375053.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   606   e-170
ref|XP_009375052.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   606   e-170
ref|XP_009375051.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   606   e-170
gb|KDO61266.1| hypothetical protein CISIN_1g042717mg, partial [C...   606   e-170
ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   606   e-170
ref|XP_011006202.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   604   e-170
ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Popu...   604   e-170
ref|XP_009376883.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   603   e-169
ref|XP_008385560.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   603   e-169
ref|XP_008385559.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   603   e-169
ref|XP_010045014.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   602   e-169
ref|XP_009347315.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   602   e-169

>ref|XP_004142116.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis
            sativus] gi|700199017|gb|KGN54175.1| hypothetical protein
            Csa_4G291390 [Cucumis sativus]
          Length = 538

 Score =  632 bits (1629), Expect = e-178
 Identities = 314/426 (73%), Positives = 353/426 (82%), Gaps = 5/426 (1%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPWD+DLKYA+AVC+Q+ VPLEVVHLT+EYW+NVVSYIIEEYRCG
Sbjct: 113  LKIWFQEDFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCG 172

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIH-SSYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMDAIS + FDYVASGHYA VIH S+ + +  S LELS DMVK
Sbjct: 173  RTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVK 232

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++LLFPLGCIPKDEVRKLA K  LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 233  DQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEF 292

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            VARH         EAE+G+FLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVS
Sbjct: 293  VARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVS 352

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFSF+KRRR FRVGSLKWL+G P DQF +L CKVRHGPSFYSC+LTME  ED  ED A
Sbjct: 353  RNYFSFDKRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVA 412

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL EDDQGLAAGQFAAFY+ ++C+GSGVILESWDDQGFPVC KA+E A+M+DKSKLGK
Sbjct: 413  VVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGK 472

Query: 360  PVKIMTKQEPSSPRNSKQK---YIVEELNKESMDVTESHIQTS-KEGLMSSSLNLFHKLK 193
            P+KI  K E S  + SK K    I ++L  E   V E   + S  E + + S+     LK
Sbjct: 473  PIKIKVKPE-SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLK 531

Query: 192  EKLMCI 175
            +KL+ I
Sbjct: 532  KKLLQI 537


>ref|XP_010102337.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis]
            gi|587905107|gb|EXB93298.1| tRNA-specific 2-thiouridylase
            mnmA [Morus notabilis]
          Length = 580

 Score =  621 bits (1602), Expect = e-175
 Identities = 309/420 (73%), Positives = 345/420 (82%), Gaps = 3/420 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA+AVC+QI VPLEVVHLTDEYWKNVVSYII+EYRCG
Sbjct: 161  LKIWFQEDFENFWSECPWEEDLKYAKAVCSQIEVPLEVVHLTDEYWKNVVSYIIDEYRCG 220

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFG+FMDAIS++ FDYVASGHYA V+HS + E    S LELS DMVK
Sbjct: 221  RTPNPDVLCNTRIKFGSFMDAISSMEFDYVASGHYANVVHSFTNERGDASVLELSQDMVK 280

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++L+FPLGCI K+EVRKLA K +LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 281  DQTYFLSHLSQAQLKRLVFPLGCISKEEVRKLAKKFDLPNKDRKDSQGICFLGKIKFSEF 340

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            VARH         EAETG+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVF S
Sbjct: 341  VARHIGEKEGIILEAETGDFLGMHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFAS 400

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFSF+KRRR FRVGSLKWLNG P DQ  QL CKVRHGP FY+CS TME GE+  ED A
Sbjct: 401  RNYFSFDKRRRIFRVGSLKWLNGLPPDQTCQLQCKVRHGPGFYNCSFTMESGENSGEDVA 460

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VV L EDDQGLA GQFAAFY+ K+CLGSG+ILESWDDQGFPVC+KALEIAKM+DKS LGK
Sbjct: 461  VVQLSEDDQGLAVGQFAAFYQGKSCLGSGIILESWDDQGFPVCEKALEIAKMEDKSLLGK 520

Query: 360  PVKIMTKQEPSSPRNSKQKYIVEELNKESMDVTESHIQTSKEG--LMSSSLNLFHKLKEK 187
            PVKI  K +P SP     +    EL++       SHI  +K+G   +S  +    +L+EK
Sbjct: 521  PVKI--KVKPESPTIEPGQKSSTELDRGQ---RSSHIAAAKQGRQALSFPMKWLQRLREK 575


>ref|XP_008449720.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis
            melo]
          Length = 539

 Score =  619 bits (1597), Expect = e-174
 Identities = 303/425 (71%), Positives = 347/425 (81%), Gaps = 4/425 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPWD+DLKYA+AVC+QI VPLEVVHLT+EYW+NVVSYIIEEYRCG
Sbjct: 114  LKIWFQEDFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCG 173

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIH-SSYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMDAIS + FDYVASGHYA VIH S+ + +  S LELS DMVK
Sbjct: 174  RTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVK 233

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++LLFPLGCIPKDEVRKLA K  LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 234  DQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEF 293

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            VARH         EAE+G+FLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKD++NNVVFVS
Sbjct: 294  VARHIGEDEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVS 353

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFSF+KRRR FRVGSLKWL+G P D+F  L CKVRHGP FYSC+LTME  +D  ED A
Sbjct: 354  RNYFSFDKRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVA 413

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL EDDQGLAAGQFAAFY+ ++C+GSGVILESWDDQGFPVC KA+E A+M+DKSKLGK
Sbjct: 414  VVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGK 473

Query: 360  PVKIMTKQEP---SSPRNSKQKYIVEELNKESMDVTESHIQTSKEGLMSSSLNLFHKLKE 190
            P+KI  K +     S      K   +  N+E+  V +    +  E + ++ +     L++
Sbjct: 474  PIKIKVKPDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRK 533

Query: 189  KLMCI 175
            KL+ I
Sbjct: 534  KLLQI 538


>ref|XP_002282827.2| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Vitis
            vinifera] gi|297746327|emb|CBI16383.3| unnamed protein
            product [Vitis vinifera]
          Length = 544

 Score =  619 bits (1595), Expect = e-174
 Identities = 307/423 (72%), Positives = 345/423 (81%), Gaps = 6/423 (1%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW++DLKYA+AVC+Q+ VPLEVVHLTDEYWKNVVSYIIEEYRCG
Sbjct: 119  LKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCG 178

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMDAIS+  FDYVASGHYA V+HS + + +  S LELS DMVK
Sbjct: 179  RTPNPDVLCNTRIKFGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVK 238

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++L+FPLGCIPKDEVR LATKL+LPN++RKDSQGICFLGKIKFSEF
Sbjct: 239  DQTYFLSHLSQIQLKRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEF 298

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            VARH         EAETG+ LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVS
Sbjct: 299  VARHIGEKEGIILEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVS 358

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS +KRRR FRVGSLKW++  P  Q +QL CKVRHGP FY+CS TMEPGED  ED A
Sbjct: 359  RNYFSLDKRRRLFRVGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVA 418

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VV L EDDQGLAAGQF AFY+ ++C+GSGVILESWDDQGFPVC KALEIA+M+DKSKLGK
Sbjct: 419  VVQLSEDDQGLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGK 478

Query: 360  PVKIMTKQEPSSPRNSKQKYIVEELNKE---SMDVTESHIQTSKEGLMSS--SLNLFHKL 196
            PVKI  K  P +P     +    +L++E   S       I  S EG   S   +N   K+
Sbjct: 479  PVKI--KVRPETPPKGSDQIDGIKLDRELANSQAAVTDEISRSSEGEARSRFPMNWLQKI 536

Query: 195  KEK 187
            +EK
Sbjct: 537  REK 539


>ref|XP_010257320.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
            [Nelumbo nucifera]
          Length = 531

 Score =  615 bits (1587), Expect = e-173
 Identities = 305/421 (72%), Positives = 347/421 (82%), Gaps = 4/421 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWSACPW+EDL+Y +AVC+Q+ VPLEVVHLTDEYW NVVSYII+EYR G
Sbjct: 110  LKIWFQEDFENFWSACPWEEDLEYVKAVCDQVEVPLEVVHLTDEYWDNVVSYIIQEYRYG 169

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAF+DAIS + FDYVASGHYA VIH  + + +G S LELS D VK
Sbjct: 170  RTPNPDVLCNTRIKFGAFLDAISNMEFDYVASGHYANVIHPCTDQKDGPSILELSEDKVK 229

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++LLFPLGCI K+EVRKLA KLELPN++RKDSQGICFLGKIKFSEF
Sbjct: 230  DQTYFLSHLSQAQLKRLLFPLGCISKNEVRKLAAKLELPNRDRKDSQGICFLGKIKFSEF 289

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            +A+H         EAETG+FLGKH GFWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVS
Sbjct: 290  IAKHIGEMEGILLEAETGDFLGKHHGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVS 349

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNY+SF+KRRRRFRVGSLKWL G P DQF++L CKVRHGP FY CSL ME GED    +A
Sbjct: 350  RNYYSFDKRRRRFRVGSLKWLAGLPPDQFNKLQCKVRHGPGFYDCSLIMETGEDGDRTSA 409

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL EDDQGLAAGQFAAFY+ KTC+GSGVILE WD QGFPVC KAL+IA+M+DKSKLGK
Sbjct: 410  VVHLSEDDQGLAAGQFAAFYQGKTCIGSGVILECWDSQGFPVCAKALQIARMEDKSKLGK 469

Query: 360  PVKIMTKQEPSSPRNSKQKYIVEELNKESM---DVTESHIQTSKEGLMSSSLNLFHKLKE 190
            PVKI  K  P +   S +     ELN +S+   D+T    + S +G+   ++N F KL+E
Sbjct: 470  PVKI--KVNPENSLKSSEITDKVELNGKSLNSEDITLDKSKQSSQGIF--NVNWFQKLRE 525

Query: 189  K 187
            K
Sbjct: 526  K 526


>ref|XP_008238179.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X1
            [Prunus mume]
          Length = 537

 Score =  609 bits (1570), Expect = e-171
 Identities = 303/427 (70%), Positives = 342/427 (80%), Gaps = 6/427 (1%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA+AVCNQ+ VPLEVVHLT+EYWKNVVSYIIEEYRCG
Sbjct: 112  LKIWFQEDFENFWSECPWEEDLKYAKAVCNQVDVPLEVVHLTNEYWKNVVSYIIEEYRCG 171

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIH-SSYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMDAIS + FDYVASGHYA V+H S+ E +  S LELS DMVK
Sbjct: 172  RTPNPDVLCNTRIKFGAFMDAISCMKFDYVASGHYANVVHPSAGEMDKDSVLELSQDMVK 231

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL+QL+FPLGC+ KDEVRKLATK +L NK+RKDSQGICFLGKIKFSEF
Sbjct: 232  DQTYFLSHLSQAQLKQLIFPLGCLSKDEVRKLATKFDLLNKQRKDSQGICFLGKIKFSEF 291

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            +ARH         EAETG+ LG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVS
Sbjct: 292  IARHIGEKEGIILEAETGDLLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVS 351

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS +KRRR FRVGSLKWLNG P +Q  QL CKVRHGP F +CSL MEPG    ED A
Sbjct: 352  RNYFSVDKRRRLFRVGSLKWLNGFPPNQISQLQCKVRHGPGFRNCSLIMEPGAYGHEDVA 411

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL EDDQGLAAGQFAAFY+ +TCLGSGVILESWDDQGFPVC +ALEIA+M+DKS LG+
Sbjct: 412  VVHLSEDDQGLAAGQFAAFYQGRTCLGSGVILESWDDQGFPVCARALEIARMEDKSLLGR 471

Query: 360  PVKIMTKQEPSSPRNSKQKY----IVEELNKESMDVTESHIQTSK-EGLMSSSLNLFHKL 196
            PVKI  K +P +P     +     +   L    +   +  +Q S+ E +    +N   K 
Sbjct: 472  PVKI--KVKPENPITESDQVDGMELCGGLKHSGISAAKQRMQVSQGEAITRFPVNWLQKF 529

Query: 195  KEKLMCI 175
            +EK + I
Sbjct: 530  REKWLHI 536


>ref|XP_004507093.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cicer
            arietinum]
          Length = 484

 Score =  609 bits (1570), Expect = e-171
 Identities = 294/374 (78%), Positives = 324/374 (86%), Gaps = 1/374 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW++DLKYA+ VCNQ+ VPLEVVHLTDEYWKNVVSYIIEEY+CG
Sbjct: 100  LKIWFQEDFENFWSECPWEDDLKYAKDVCNQVEVPLEVVHLTDEYWKNVVSYIIEEYQCG 159

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIH-SSYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAF+DAIS +GFDYVASGHYA VIH S+ + +G S LELS DMVK
Sbjct: 160  RTPNPDVLCNTRIKFGAFLDAISDMGFDYVASGHYANVIHPSADQMDGPSTLELSQDMVK 219

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL+QLLFPLGCIPKDEVR+LATK +LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 220  DQTYFLSHLSQSQLKQLLFPLGCIPKDEVRRLATKFDLPNKDRKDSQGICFLGKIKFSEF 279

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            VARH         EAETG+FLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVS
Sbjct: 280  VARHIGEKEGIILEAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVS 339

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS +KRRR FRVGSLKWL+G P  Q  QL CKVRHGP FY CSL ME   +   D+A
Sbjct: 340  RNYFSIDKRRRVFRVGSLKWLSGLPPSQTSQLRCKVRHGPGFYDCSLQMELEGENPIDSA 399

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VV + EDDQGLAAGQF AFY  +TC+GSGVILESWDDQGFPVC KALEIA+M+DKSK+G 
Sbjct: 400  VVRISEDDQGLAAGQFTAFYEGRTCIGSGVILESWDDQGFPVCTKALEIARMEDKSKIGN 459

Query: 360  PVKIMTKQEPSSPR 319
            PVKI  K +P +P+
Sbjct: 460  PVKI--KVKPDNPQ 471


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
            tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis]
          Length = 497

 Score =  606 bits (1563), Expect = e-170
 Identities = 295/395 (74%), Positives = 332/395 (84%), Gaps = 3/395 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA+AVC+Q+ +PLEVVHLTDEYWKNVVSYIIEEYRCG
Sbjct: 102  LKIWFQEDFENFWSECPWEEDLKYAKAVCDQVDIPLEVVHLTDEYWKNVVSYIIEEYRCG 161

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIH-SSYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMDAI+ + FDYVASGHYA+VIH S+ E N  S LELS DMVK
Sbjct: 162  RTPNPDVLCNTRIKFGAFMDAINNMEFDYVASGHYAKVIHPSADEINQPSVLELSKDMVK 221

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QLR+L+FPLG I KDEVRKLAT   LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 222  DQTYFLSHLSQAQLRRLIFPLGGISKDEVRKLATLFNLPNKDRKDSQGICFLGKIKFSEF 281

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            VARH         EAETG+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVS
Sbjct: 282  VARHIGEMEGIMLEAETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVS 341

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFSF+KRRR  RVGSL+W NG P  +  +L CKVRHGP FY+CS+ +EPGE   +D A
Sbjct: 342  RNYFSFDKRRRLLRVGSLRWFNGSPPKETSELQCKVRHGPGFYNCSVVIEPGEVDDQDVA 401

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            +V LCEDDQGLAAGQFAAFY+ +TC+GSGVILESW+DQGFPVC++ALE+A+++DKS+LGK
Sbjct: 402  IVQLCEDDQGLAAGQFAAFYKGRTCIGSGVILESWNDQGFPVCERALELARIEDKSELGK 461

Query: 360  PVKIMTKQE-PSSPRNSKQKYIVEE-LNKESMDVT 262
            PVKI  K E P    + K    VE  LN   + VT
Sbjct: 462  PVKIKVKPEIPVREFDGKDGIEVERGLNNSQVAVT 496


>ref|XP_009375053.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X3
            [Pyrus x bretschneideri]
          Length = 512

 Score =  606 bits (1562), Expect = e-170
 Identities = 290/369 (78%), Positives = 321/369 (86%), Gaps = 1/369 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA AVCNQ+ VPLEVVHLTDEYWKNVVSYIIEEY+CG
Sbjct: 104  LKIWFQEDFENFWSECPWEEDLKYANAVCNQVDVPLEVVHLTDEYWKNVVSYIIEEYQCG 163

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFG+FMDAIS++ FD VASGHYA V+H  + E +  S LELS DMVK
Sbjct: 164  RTPNPDVLCNTRIKFGSFMDAISSMKFDRVASGHYANVVHPPASETDKDSVLELSQDMVK 223

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++L+FPLGC+ KDEVRKLATK +LPNK+RKDSQGICFLGKIKF+EF
Sbjct: 224  DQTYFLSHLSQAQLKRLIFPLGCLSKDEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEF 283

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            +ARH         EAE+G+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVS
Sbjct: 284  IARHIGENKGVILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVS 343

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS EKRR  FRVGSLKWLNG P +Q  QL CKVRHGP+F +CSL MEPGED  ED A
Sbjct: 344  RNYFSDEKRRHLFRVGSLKWLNGLPPNQISQLECKVRHGPAFCNCSLIMEPGEDGHEDVA 403

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL +DDQGLAAGQFAAFYR +TCLGSGVILESWDDQGFP+C  ALEIA+++DKS LGK
Sbjct: 404  VVHLSQDDQGLAAGQFAAFYRGRTCLGSGVILESWDDQGFPMCAIALEIARLEDKSLLGK 463

Query: 360  PVKIMTKQE 334
            PVKI  K E
Sbjct: 464  PVKIKVKPE 472


>ref|XP_009375052.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X2
            [Pyrus x bretschneideri]
          Length = 525

 Score =  606 bits (1562), Expect = e-170
 Identities = 290/369 (78%), Positives = 321/369 (86%), Gaps = 1/369 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA AVCNQ+ VPLEVVHLTDEYWKNVVSYIIEEY+CG
Sbjct: 104  LKIWFQEDFENFWSECPWEEDLKYANAVCNQVDVPLEVVHLTDEYWKNVVSYIIEEYQCG 163

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFG+FMDAIS++ FD VASGHYA V+H  + E +  S LELS DMVK
Sbjct: 164  RTPNPDVLCNTRIKFGSFMDAISSMKFDRVASGHYANVVHPPASETDKDSVLELSQDMVK 223

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++L+FPLGC+ KDEVRKLATK +LPNK+RKDSQGICFLGKIKF+EF
Sbjct: 224  DQTYFLSHLSQAQLKRLIFPLGCLSKDEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEF 283

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            +ARH         EAE+G+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVS
Sbjct: 284  IARHIGENKGVILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVS 343

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS EKRR  FRVGSLKWLNG P +Q  QL CKVRHGP+F +CSL MEPGED  ED A
Sbjct: 344  RNYFSDEKRRHLFRVGSLKWLNGLPPNQISQLECKVRHGPAFCNCSLIMEPGEDGHEDVA 403

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL +DDQGLAAGQFAAFYR +TCLGSGVILESWDDQGFP+C  ALEIA+++DKS LGK
Sbjct: 404  VVHLSQDDQGLAAGQFAAFYRGRTCLGSGVILESWDDQGFPMCAIALEIARLEDKSLLGK 463

Query: 360  PVKIMTKQE 334
            PVKI  K E
Sbjct: 464  PVKIKVKPE 472


>ref|XP_009375051.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X1
            [Pyrus x bretschneideri]
          Length = 527

 Score =  606 bits (1562), Expect = e-170
 Identities = 290/369 (78%), Positives = 321/369 (86%), Gaps = 1/369 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA AVCNQ+ VPLEVVHLTDEYWKNVVSYIIEEY+CG
Sbjct: 104  LKIWFQEDFENFWSECPWEEDLKYANAVCNQVDVPLEVVHLTDEYWKNVVSYIIEEYQCG 163

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFG+FMDAIS++ FD VASGHYA V+H  + E +  S LELS DMVK
Sbjct: 164  RTPNPDVLCNTRIKFGSFMDAISSMKFDRVASGHYANVVHPPASETDKDSVLELSQDMVK 223

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++L+FPLGC+ KDEVRKLATK +LPNK+RKDSQGICFLGKIKF+EF
Sbjct: 224  DQTYFLSHLSQAQLKRLIFPLGCLSKDEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEF 283

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            +ARH         EAE+G+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVS
Sbjct: 284  IARHIGENKGVILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVS 343

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS EKRR  FRVGSLKWLNG P +Q  QL CKVRHGP+F +CSL MEPGED  ED A
Sbjct: 344  RNYFSDEKRRHLFRVGSLKWLNGLPPNQISQLECKVRHGPAFCNCSLIMEPGEDGHEDVA 403

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL +DDQGLAAGQFAAFYR +TCLGSGVILESWDDQGFP+C  ALEIA+++DKS LGK
Sbjct: 404  VVHLSQDDQGLAAGQFAAFYRGRTCLGSGVILESWDDQGFPMCAIALEIARLEDKSLLGK 463

Query: 360  PVKIMTKQE 334
            PVKI  K E
Sbjct: 464  PVKIKVKPE 472


>gb|KDO61266.1| hypothetical protein CISIN_1g042717mg, partial [Citrus sinensis]
          Length = 474

 Score =  606 bits (1562), Expect = e-170
 Identities = 294/420 (70%), Positives = 343/420 (81%), Gaps = 3/420 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFW+ CPW+ED+KYA+AVC+Q+ VPLEVVHLTDEYWKNVVSYIIEEYRCG
Sbjct: 50   LKIWFQEDFENFWTECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCG 109

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHSSYEAN-GTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMDAIS++ FDYVASGHYA+V+HSS +     S LELS D VK
Sbjct: 110  RTPNPDVLCNTRIKFGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVK 169

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++L+FPLGCI K+EVRK AT+ +LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 170  DQTYFLSHLSQAQLKRLIFPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEF 229

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            VARH         EAETG+FLGKH GFWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVS
Sbjct: 230  VARHIGEMEGVILEAETGDFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVS 289

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNY+SF+K+RR FRVGSLKWL+G P ++  +L CKVRHGP FY+C+L +E  ED  E+ A
Sbjct: 290  RNYYSFDKKRRLFRVGSLKWLSGLPPEEISRLQCKVRHGPGFYNCNLMIEHVEDGGENIA 349

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VV L EDDQGLAAGQFAAFY+   C+GSGVILESWDDQGFPVC+KALEIA+M+DKSKLGK
Sbjct: 350  VVQLSEDDQGLAAGQFAAFYQGTRCIGSGVILESWDDQGFPVCEKALEIARMEDKSKLGK 409

Query: 360  PVKIMTKQEPSSPRNSKQKYIVEE--LNKESMDVTESHIQTSKEGLMSSSLNLFHKLKEK 187
            P+KI  K E     NS +        +N ++    +S+  + +E +    +N   KL+EK
Sbjct: 410  PIKIKVKPETPEEFNSNKGVQSSRNLVNAQNSIAEKSNTTSQEEAISRFPMNWLQKLREK 469


>ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Citrus
            sinensis]
          Length = 525

 Score =  606 bits (1562), Expect = e-170
 Identities = 294/420 (70%), Positives = 343/420 (81%), Gaps = 3/420 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFW+ CPW+ED+KYA+AVC+Q+ VPLEVVHLTDEYWKNVVSYIIEEYRCG
Sbjct: 101  LKIWFQEDFENFWTECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCG 160

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHSSYEAN-GTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMDAIS++ FDYVASGHYA+V+HSS +     S LELS D VK
Sbjct: 161  RTPNPDVLCNTRIKFGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVK 220

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++L+FPLGCI K+EVRK AT+ +LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 221  DQTYFLSHLSQAQLKRLIFPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEF 280

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            VARH         EAETG+FLGKH GFWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVS
Sbjct: 281  VARHIGEMEGVILEAETGDFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVS 340

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNY+SF+K+RR FRVGSLKWL+G P ++  +L CKVRHGP FY+C+L +E  ED  E+ A
Sbjct: 341  RNYYSFDKKRRLFRVGSLKWLSGLPPEEISRLQCKVRHGPGFYNCNLMIEHVEDGGENIA 400

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VV L EDDQGLAAGQFAAFY+   C+GSGVILESWDDQGFPVC+KALEIA+M+DKSKLGK
Sbjct: 401  VVQLSEDDQGLAAGQFAAFYQGTRCIGSGVILESWDDQGFPVCEKALEIARMEDKSKLGK 460

Query: 360  PVKIMTKQEPSSPRNSKQKYIVEE--LNKESMDVTESHIQTSKEGLMSSSLNLFHKLKEK 187
            P+KI  K E     NS +        +N ++    +S+  + +E +    +N   KL+EK
Sbjct: 461  PIKIKVKPETPEEFNSNKGVQSSRNLVNAQNSIAEKSNTTSQEEAISRFPMNWLQKLREK 520


>ref|XP_011006202.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Populus
            euphratica]
          Length = 512

 Score =  604 bits (1557), Expect = e-170
 Identities = 287/379 (75%), Positives = 324/379 (85%), Gaps = 1/379 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ NFWS CPW+EDLK+A+ VC+Q+GVPLEVVHLTDEYW NVVSYIIEEYRCG
Sbjct: 117  LKIWFQEDFNNFWSECPWEEDLKFAKGVCDQVGVPLEVVHLTDEYWSNVVSYIIEEYRCG 176

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAF+DAI+ + FDYVASGHYARVIH  S + N  S LELS D +K
Sbjct: 177  RTPNPDVLCNTRIKFGAFIDAINNMEFDYVASGHYARVIHPVSNQINKPSVLELSKDKLK 236

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHL+Q QL++L+FPLGCIPK+EVR LA+K +LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 237  DQTYFLSHLAQAQLKRLIFPLGCIPKEEVRNLASKFDLPNKDRKDSQGICFLGKIKFSEF 296

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            V RH         EAETG+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVS
Sbjct: 297  VVRHIGESEGVILEAETGDFLGIHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVS 356

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS +KRRR FRVGSLKWL+G P D   Q  CKVRHGP+FY CS+ +E GED  E+  
Sbjct: 357  RNYFSLDKRRRSFRVGSLKWLSGSPPDMTSQFQCKVRHGPTFYDCSVKIELGEDSPEEVV 416

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHLCEDDQGLAAGQF AFY+ +TC+GSGVILESWDD+GFPVC KALE+A+++DKSKLGK
Sbjct: 417  VVHLCEDDQGLAAGQFTAFYQGRTCIGSGVILESWDDKGFPVCQKALELARLEDKSKLGK 476

Query: 360  PVKIMTKQEPSSPRNSKQK 304
            PVKI  K E +S + S QK
Sbjct: 477  PVKIKVKPE-TSDQESVQK 494


>ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Populus trichocarpa]
            gi|550348245|gb|EEE83209.2| hypothetical protein
            POPTR_0001s26620g [Populus trichocarpa]
          Length = 512

 Score =  604 bits (1557), Expect = e-170
 Identities = 288/383 (75%), Positives = 325/383 (84%), Gaps = 1/383 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ NFWS CPW+EDLK+A+ VC+Q+GVPLEVVHLTDEYW NVVSYIIEEYRCG
Sbjct: 117  LKIWFQEDFNNFWSECPWEEDLKFAKGVCDQVGVPLEVVHLTDEYWSNVVSYIIEEYRCG 176

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMDAI+ + FDYVASGHYA VIH  S + N  S LELS D +K
Sbjct: 177  RTPNPDVLCNTRIKFGAFMDAINNMEFDYVASGHYAHVIHPVSDQINKPSVLELSKDKLK 236

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHL+Q QL++L+FPLGCIPK+EVR LA+K +LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 237  DQTYFLSHLTQAQLKRLIFPLGCIPKEEVRNLASKFDLPNKDRKDSQGICFLGKIKFSEF 296

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            V RH         EAETG+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVS
Sbjct: 297  VGRHIGESEGVILEAETGDFLGIHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVS 356

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS +KRRR FRVGSLKWL+G P D   QL CKVRHGP+FY CS+ +E GED  E+  
Sbjct: 357  RNYFSLDKRRRSFRVGSLKWLSGSPTDMTTQLQCKVRHGPTFYDCSVKIELGEDSPEEVV 416

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
             VHLCEDDQGLAAGQF AFY+ +TC+GSGVILESWDD+GFPVC KALE+A+++DKSKLGK
Sbjct: 417  AVHLCEDDQGLAAGQFTAFYQGRTCIGSGVILESWDDKGFPVCQKALELARLEDKSKLGK 476

Query: 360  PVKIMTKQEPSSPRNSKQKYIVE 292
            PVKI  K E +S + S QK  +E
Sbjct: 477  PVKIKVKPE-TSDQESVQKDGIE 498


>ref|XP_009376883.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
            X1 [Pyrus x bretschneideri]
          Length = 579

 Score =  603 bits (1555), Expect = e-169
 Identities = 288/369 (78%), Positives = 321/369 (86%), Gaps = 1/369 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA+AVCNQ+ VPLEVVHLTDEYWKNVVSYIIEEY+CG
Sbjct: 177  LKIWFQEDFENFWSECPWEEDLKYAKAVCNQVDVPLEVVHLTDEYWKNVVSYIIEEYQCG 236

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMD+I+++ FDYVASGHYA V+H  + + +  S LELS DMVK
Sbjct: 237  RTPNPDVLCNTRIKFGAFMDSINSMKFDYVASGHYANVVHPPASQTDKDSVLELSQDMVK 296

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLS+LSQ QLR+L+FPLGC+ KDEVRKLATK +LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 297  DQTYFLSNLSQAQLRRLIFPLGCLSKDEVRKLATKFDLPNKDRKDSQGICFLGKIKFSEF 356

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            +ARH         EAE+G+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVS
Sbjct: 357  IARHIGEKEGVILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVS 416

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS +KRRR FRVGSLKWLNG   +Q  QL CKVRHGP+F  CS  MEPGED  ED A
Sbjct: 417  RNYFSVDKRRRLFRVGSLKWLNGLSPNQISQLECKVRHGPAFCKCSFIMEPGEDGHEDVA 476

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL +DDQGLAAGQFAAFY+ +TCLGSGVILESWDDQGFPVC +A EIA+M+DKS LGK
Sbjct: 477  VVHLSQDDQGLAAGQFAAFYQGRTCLGSGVILESWDDQGFPVCARAHEIARMEDKSILGK 536

Query: 360  PVKIMTKQE 334
            PVKI  K E
Sbjct: 537  PVKIKVKPE 545


>ref|XP_008385560.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
            X2 [Malus domestica]
          Length = 522

 Score =  603 bits (1554), Expect = e-169
 Identities = 287/369 (77%), Positives = 321/369 (86%), Gaps = 1/369 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA AVCNQ+ VPLEVVHLTDEYWKNVVSYII  Y+CG
Sbjct: 114  LKIWFQEDFENFWSECPWEEDLKYANAVCNQVXVPLEVVHLTDEYWKNVVSYIIXXYQCG 173

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMDAIS++ FD+VASGHYA V+H  + + +  S LELS DMVK
Sbjct: 174  RTPNPDVLCNTRIKFGAFMDAISSMKFDHVASGHYANVVHPPASQTDKDSVLELSQDMVK 233

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++L+FPLGC+ KDEVRKLATK +LPNK+RKDSQGICFLGKIKF+EF
Sbjct: 234  DQTYFLSHLSQAQLKRLIFPLGCLSKDEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEF 293

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            +ARH         EAE+G+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVS
Sbjct: 294  IARHIGEKEGVILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVS 353

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS +KRRR FRVGSLKWLNG P +Q  QL CKVRHGP+F +CSL MEPGED  ED A
Sbjct: 354  RNYFSDKKRRRLFRVGSLKWLNGLPPNQISQLECKVRHGPAFCNCSLIMEPGEDGHEDVA 413

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL +DDQGLAAGQFAAFY+  TCLGSGVILESWDDQGFPVC +A+EIA+++DKS LGK
Sbjct: 414  VVHLSQDDQGLAAGQFAAFYQGXTCLGSGVILESWDDQGFPVCARAIEIARLEDKSLLGK 473

Query: 360  PVKIMTKQE 334
            PVKI  K E
Sbjct: 474  PVKIKVKPE 482


>ref|XP_008385559.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
            X1 [Malus domestica]
          Length = 549

 Score =  603 bits (1554), Expect = e-169
 Identities = 287/369 (77%), Positives = 321/369 (86%), Gaps = 1/369 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA AVCNQ+ VPLEVVHLTDEYWKNVVSYII  Y+CG
Sbjct: 114  LKIWFQEDFENFWSECPWEEDLKYANAVCNQVXVPLEVVHLTDEYWKNVVSYIIXXYQCG 173

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMDAIS++ FD+VASGHYA V+H  + + +  S LELS DMVK
Sbjct: 174  RTPNPDVLCNTRIKFGAFMDAISSMKFDHVASGHYANVVHPPASQTDKDSVLELSQDMVK 233

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLSHLSQ QL++L+FPLGC+ KDEVRKLATK +LPNK+RKDSQGICFLGKIKF+EF
Sbjct: 234  DQTYFLSHLSQAQLKRLIFPLGCLSKDEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEF 293

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            +ARH         EAE+G+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVS
Sbjct: 294  IARHIGEKEGVILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVS 353

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS +KRRR FRVGSLKWLNG P +Q  QL CKVRHGP+F +CSL MEPGED  ED A
Sbjct: 354  RNYFSDKKRRRLFRVGSLKWLNGLPPNQISQLECKVRHGPAFCNCSLIMEPGEDGHEDVA 413

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL +DDQGLAAGQFAAFY+  TCLGSGVILESWDDQGFPVC +A+EIA+++DKS LGK
Sbjct: 414  VVHLSQDDQGLAAGQFAAFYQGXTCLGSGVILESWDDQGFPVCARAIEIARLEDKSLLGK 473

Query: 360  PVKIMTKQE 334
            PVKI  K E
Sbjct: 474  PVKIKVKPE 482


>ref|XP_010045014.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X1
            [Eucalyptus grandis]
          Length = 495

 Score =  602 bits (1552), Expect = e-169
 Identities = 283/368 (76%), Positives = 321/368 (87%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA+AVC Q+ VPLEVVHLTDEYWKNVVSYIIEEYR G
Sbjct: 124  LKIWFQEDFENFWSECPWEEDLKYAKAVCEQVDVPLEVVHLTDEYWKNVVSYIIEEYRSG 183

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHSSYEANGTSRLELSGDMVKD 1078
            RTPNPDVLCNTRIKFGAFMDAI+++ +D++ASGHYA+V+HSS   +  S L+LS DMVKD
Sbjct: 184  RTPNPDVLCNTRIKFGAFMDAINSMEYDFIASGHYAKVVHSSNHIDNASELKLSEDMVKD 243

Query: 1077 QTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEFV 898
            QTYFLSHLSQ QL++L+FPLGCI K+EVRKLA + +LPN++RKDSQGICFLGKIKFSEFV
Sbjct: 244  QTYFLSHLSQAQLKRLIFPLGCISKNEVRKLAARFDLPNQDRKDSQGICFLGKIKFSEFV 303

Query: 897  ARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVSR 718
            ARH         EAETG+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD +NNVVFVSR
Sbjct: 304  ARHIGEMEGIILEAETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSR 363

Query: 717  NYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAAV 538
            NYFS +KRRR FRVGSLKWL G P  +  QL CKVRHGP FY+CSL++E GE+ +ED AV
Sbjct: 364  NYFSVDKRRRLFRVGSLKWLIGAPPTEISQLQCKVRHGPGFYNCSLSLELGENGQEDVAV 423

Query: 537  VHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGKP 358
            V L EDDQGLAAGQF AFY+++TC+GSGVILESW+DQGFPVC KALEIA+M+DKSKLGKP
Sbjct: 424  VQLPEDDQGLAAGQFTAFYQDRTCIGSGVILESWNDQGFPVCQKALEIARMEDKSKLGKP 483

Query: 357  VKIMTKQE 334
            VKI  K E
Sbjct: 484  VKIKVKPE 491


>ref|XP_009347315.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Pyrus
            x bretschneideri] gi|694441086|ref|XP_009347316.1|
            PREDICTED: mitochondrial tRNA-specific 2-thiouridylase
            1-like [Pyrus x bretschneideri]
          Length = 588

 Score =  602 bits (1552), Expect = e-169
 Identities = 287/369 (77%), Positives = 321/369 (86%), Gaps = 1/369 (0%)
 Frame = -1

Query: 1437 LKIWFQEDYENFWSACPWDEDLKYAEAVCNQIGVPLEVVHLTDEYWKNVVSYIIEEYRCG 1258
            LKIWFQED+ENFWS CPW+EDLKYA+AVCNQ+ VPLEVVHLTDEYWKNVVSYIIEEY+CG
Sbjct: 116  LKIWFQEDFENFWSECPWEEDLKYAKAVCNQVDVPLEVVHLTDEYWKNVVSYIIEEYQCG 175

Query: 1257 RTPNPDVLCNTRIKFGAFMDAISTLGFDYVASGHYARVIHS-SYEANGTSRLELSGDMVK 1081
            RTPNPDVLCNTRIKFGAFMD+I+++ FDYVASGHYA V+H  + + +  S LELS DMVK
Sbjct: 176  RTPNPDVLCNTRIKFGAFMDSINSMKFDYVASGHYANVVHPPASQTDKDSVLELSQDMVK 235

Query: 1080 DQTYFLSHLSQGQLRQLLFPLGCIPKDEVRKLATKLELPNKERKDSQGICFLGKIKFSEF 901
            DQTYFLS+LSQ QLR+L+FPLGC+ KDEVRKLATK +LPNK+RKDSQGICFLGKIKFSEF
Sbjct: 236  DQTYFLSNLSQAQLRRLIFPLGCLSKDEVRKLATKFDLPNKDRKDSQGICFLGKIKFSEF 295

Query: 900  VARHXXXXXXXXXEAETGNFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVS 721
            +ARH         EAE+G+FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVS
Sbjct: 296  IARHIGEKEGVILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVS 355

Query: 720  RNYFSFEKRRRRFRVGSLKWLNGPPVDQFDQLLCKVRHGPSFYSCSLTMEPGEDCKEDAA 541
            RNYFS +KRRR FRVGSLKWLNG   +Q  QL CKVRHGP+F  CS  MEPGED  ED A
Sbjct: 356  RNYFSVDKRRRLFRVGSLKWLNGLSPNQISQLECKVRHGPAFCKCSFIMEPGEDGHEDVA 415

Query: 540  VVHLCEDDQGLAAGQFAAFYREKTCLGSGVILESWDDQGFPVCDKALEIAKMKDKSKLGK 361
            VVHL +DDQGLAAGQFAAFY+ +TCLGSGVILESWDDQGFPVC +A EIA+M+DKS LGK
Sbjct: 416  VVHLSQDDQGLAAGQFAAFYQGRTCLGSGVILESWDDQGFPVCARAHEIARMEDKSLLGK 475

Query: 360  PVKIMTKQE 334
            PVKI  + E
Sbjct: 476  PVKIKVEPE 484