BLASTX nr result
ID: Papaver31_contig00018392
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver31_contig00018392
(1026 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 545 e-152
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 545 e-152
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 543 e-152
sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 513 e-142
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 489 e-135
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 489 e-135
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 487 e-135
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 444 e-122
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 436 e-119
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 422 e-115
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 276 1e-71
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 276 1e-71
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 276 2e-71
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum] 275 4e-71
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 273 2e-70
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 271 5e-70
ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 266 3e-68
ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 264 8e-68
ref|XP_009610421.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 263 2e-67
ref|XP_009757123.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 261 5e-67
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 545 bits (1405), Expect = e-152
Identities = 270/341 (79%), Positives = 305/341 (89%)
Frame = -2
Query: 1025 ALIAIVITSLFYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAQLAGTYGDLFTL 846
+L+A+VIT+ YLIFRDSS PIVGNLLQLGEKPHSQFA+LA TYGDLFTL
Sbjct: 6 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTL 65
Query: 845 KLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWKSLRK 666
KLG++TVVVASTPLAASEILKTHDRVLSGRYVFQSFR+K HVEN +VWS+CNETWK LRK
Sbjct: 66 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 125
Query: 665 VCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLIFSQN 486
VCR ELFT+KMIESQA++RE KAME+VE+L+++QG+EVKIVEV+F TLVNIFGNLIFSQN
Sbjct: 126 VCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQN 185
Query: 485 IFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEIYGVW 306
IFKL DE+SGSVEMKE +WR+LELGNSTNPADYFPFLG DL GQRKDVAD LQ IY VW
Sbjct: 186 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVW 245
Query: 305 GPILTEKKLAKQHNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEWALAE 126
G +L E+K+AK HN + NDFV++LLDSGL+DQQINALLMEIFGAGTETSASTIEWAL+E
Sbjct: 246 GAMLKERKIAKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSE 305
Query: 125 LIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
L KNPE+TAN+RSELLSV+GKR VKESDIPN+PYLQAFVKE
Sbjct: 306 LTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKE 346
>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3
gi|6176562|gb|AAF05621.1|AF191772_1
(S)-N-methylcoclaurine 3'-hydroxylase [Papaver
somniferum]
Length = 481
Score = 545 bits (1404), Expect = e-152
Identities = 271/341 (79%), Positives = 303/341 (88%)
Frame = -2
Query: 1025 ALIAIVITSLFYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAQLAGTYGDLFTL 846
+L+A+VIT+ YLIFRDSS PIVGNLLQLGEKPHSQFAQLA TYGDLF+L
Sbjct: 1 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSL 60
Query: 845 KLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWKSLRK 666
KLG++TVVVASTPLAASEILKTHDRVLSGRYVFQSFR+K HVEN +VWS+CNETWK LRK
Sbjct: 61 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 120
Query: 665 VCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLIFSQN 486
VCRTELFT+KMIESQA+VRE KAME+VEYL+K+ GNEVKI EV+F TLVNIFGNLIFSQN
Sbjct: 121 VCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 180
Query: 485 IFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEIYGVW 306
IFKL DE+SGSVEMKE +WR+LELGNSTNPADYFPFLG DL GQ KDVAD LQ IY VW
Sbjct: 181 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240
Query: 305 GPILTEKKLAKQHNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEWALAE 126
G +L E+K+AKQHN + NDFV++LLDSGL+DQQINALLMEIFGAGTETSASTIEWAL+E
Sbjct: 241 GAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSE 300
Query: 125 LIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
L KNP++TAN+R ELLSV+GKR VKESDIPN+PYLQAFVKE
Sbjct: 301 LTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKE 341
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 543 bits (1399), Expect = e-152
Identities = 270/341 (79%), Positives = 302/341 (88%)
Frame = -2
Query: 1025 ALIAIVITSLFYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAQLAGTYGDLFTL 846
+L+A+VIT+ YLIFRDSS PIVGNLLQLGEKPHSQFAQLA TYGDLF+L
Sbjct: 1 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSL 60
Query: 845 KLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWKSLRK 666
KLG++TVVVASTPLAASEILKTHDRVLSGRYVFQSFR+K HVEN +VWS+CNETWK LRK
Sbjct: 61 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 120
Query: 665 VCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLIFSQN 486
VCRT+LFT+KMIESQA+VRE KAME+VEYL+K+ GNEVKI EV+F TLVNIFGNLIFSQN
Sbjct: 121 VCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 180
Query: 485 IFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEIYGVW 306
IFKL DE+SGSVEMKE +WR+LELGNSTNPADYFPFLG DL GQ KDVAD LQ IY VW
Sbjct: 181 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240
Query: 305 GPILTEKKLAKQHNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEWALAE 126
G +L E K+AKQHN + NDFV++LLDSGL+DQQINALLMEIFGAGTETSASTIEWAL+E
Sbjct: 241 GAMLKESKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSE 300
Query: 125 LIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
L KNP++TAN+R ELLSV+GKR VKESDIPN+PYLQAFVKE
Sbjct: 301 LTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKE 341
>sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
AltName: Full=Cytochrome P450 80BX
gi|388571234|gb|AFK73713.1| cytochrome P450 [Papaver
somniferum]
Length = 440
Score = 513 bits (1320), Expect = e-142
Identities = 258/342 (75%), Positives = 297/342 (86%), Gaps = 1/342 (0%)
Frame = -2
Query: 1025 ALIAIVITSLFYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAQLAGTYGDLFTL 846
ALIAIV T+ YLI R+SS PIVGNLLQLGEKPHSQFAQLA TYGDLFTL
Sbjct: 7 ALIAIVFTTFLYLIVRESSPNGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFTL 66
Query: 845 KLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWKSLRK 666
KLGTQTVVVASTPLAASE+LK HDR L GRYVFQSFR+KNHVEN +VW++CNETWK LRK
Sbjct: 67 KLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNETWKKLRK 126
Query: 665 VCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLIFSQN 486
VCRT+LFT+KMIE+QA+VRE K ME+V+YL+K++G EVKIVEVIF TLVN+FGNLIFSQ+
Sbjct: 127 VCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLIFSQD 186
Query: 485 IFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEIYGVW 306
IFKL DE+SGS+EMK+ IWR+LELGNS NP DY P LGSLDL GQRKDVAD LQ +YGVW
Sbjct: 187 IFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQGVYGVW 246
Query: 305 GPILTEKKLAK-QHNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEWALA 129
G +L E+++AK Q N +T NDFVD+LLDSGL+DQQIN+LL+++F AGTET+ASTIEWAL
Sbjct: 247 GAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTASTIEWALT 306
Query: 128 ELIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
EL KNP++TA+IRSELLSV+GKR VKESDI NLPYLQAFVKE
Sbjct: 307 ELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKE 348
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 489 bits (1260), Expect = e-135
Identities = 246/342 (71%), Positives = 284/342 (83%), Gaps = 1/342 (0%)
Frame = -2
Query: 1025 ALIAIVITSLFYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAQLAGTYGDLFTL 846
ALIA++I+S+ YL+F S PIVGNLLQLGEKPH+QFA+LA TYGD+FTL
Sbjct: 7 ALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTL 66
Query: 845 KLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWKSLRK 666
K+GT+TVVVAST AASEILKTHDR+LS RYVFQSFR+K HVEN +VWSDC ETWK+LRK
Sbjct: 67 KMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRK 126
Query: 665 VCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLIFSQN 486
VCRTELFT+KMIESQA VRE K E+VEYL K QG EVKIVEVIF TLVNIFGNLIFSQN
Sbjct: 127 VCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQN 186
Query: 485 IFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEIYGVW 306
IF+L D NSGS E KE +WR+LELGNSTNPADYFP LG DL GQRK+VA+ L+ IY +W
Sbjct: 187 IFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIW 246
Query: 305 GPILTEKKLAKQ-HNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEWALA 129
G +L E+KLAK+ + NDFVD+ LDSGLND QINALLME+FGAGTETSASTIEWA+
Sbjct: 247 GAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMT 306
Query: 128 ELIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
EL KNP++TA +RSEL +V+G+R+VKESD PNLPYL+A VKE
Sbjct: 307 ELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKE 348
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1
gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 487
Score = 489 bits (1259), Expect = e-135
Identities = 246/342 (71%), Positives = 284/342 (83%), Gaps = 1/342 (0%)
Frame = -2
Query: 1025 ALIAIVITSLFYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAQLAGTYGDLFTL 846
ALIA++I+S+ YL+F S PIVGNLLQLGEKPH+QFA+LA TYGD+FTL
Sbjct: 6 ALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTL 65
Query: 845 KLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWKSLRK 666
K+GT+TVVVAST AASEILKTHDR+LS RYVFQSFR+K HVEN +VWSDC ETWK+LRK
Sbjct: 66 KMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRK 125
Query: 665 VCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLIFSQN 486
VCRTELFT+KMIESQA VRE K E+VEYL K QG EVKIVEVIF TLVNIFGNLIFSQN
Sbjct: 126 VCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQN 185
Query: 485 IFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEIYGVW 306
IF+L D NSGS E KE +WR+LELGNSTNPADYFP LG DL GQRK+VA+ L+ IY +W
Sbjct: 186 IFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIW 245
Query: 305 GPILTEKKLAKQ-HNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEWALA 129
G +L E+KLAK+ + NDFVD+ LDSGLND QINALLME+FGAGTETSASTIEWA+
Sbjct: 246 GAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMT 305
Query: 128 ELIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
EL KNP++TA IRSE+ +V+G+R+VKESD PNLPYL+A VKE
Sbjct: 306 ELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKE 347
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2
gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 488
Score = 487 bits (1253), Expect = e-135
Identities = 245/342 (71%), Positives = 283/342 (82%), Gaps = 1/342 (0%)
Frame = -2
Query: 1025 ALIAIVITSLFYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAQLAGTYGDLFTL 846
ALIA++I+S+ YL+F S PIVGNLLQLGEKPH+QFA+LA TYGD+FTL
Sbjct: 7 ALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTL 66
Query: 845 KLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWKSLRK 666
K+GT+TVVVAST AASEILKTHDR+LS RYVFQSFR+K HVEN +VWSDC ETWK+LRK
Sbjct: 67 KMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRK 126
Query: 665 VCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLIFSQN 486
VCRTELFT+KMIESQA VRE K E+VEYL K QG EVKIVEVIF TLVNIFGNLIFSQN
Sbjct: 127 VCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQN 186
Query: 485 IFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEIYGVW 306
IF+L NSGS E KE +WR+LELGNSTNPADYFP LG DL GQRK+VA+ L+ IY +W
Sbjct: 187 IFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIW 246
Query: 305 GPILTEKKLAKQ-HNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEWALA 129
G +L E+KLAK+ + NDFVD+ LDSGLND QINALLME+FGAGTETSASTIEWA+
Sbjct: 247 GAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMT 306
Query: 128 ELIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
EL KNP++TA +RSEL +V+G+R+VKESD PNLPYL+A VKE
Sbjct: 307 ELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKE 348
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 [Coptis japonica]
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 444 bits (1142), Expect = e-122
Identities = 220/345 (63%), Positives = 279/345 (80%), Gaps = 5/345 (1%)
Frame = -2
Query: 1022 LIAIVITSLFYLIF-----RDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAQLAGTYGD 858
+++I I S +L+F RDS PIVGNLLQLG+KPH++FA+LA YG+
Sbjct: 3 VLSIAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGE 62
Query: 857 LFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWK 678
LF+LKLG+QTVVVAS+P AA+EILKTHD++LSGRYVFQSFR+K HVEN +VWS+CN+ WK
Sbjct: 63 LFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWK 122
Query: 677 SLRKVCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLI 498
LRKVCRTELFT KMIESQ+++RE KA E+V++LR +G VKIVEV+F TLVNIFGNLI
Sbjct: 123 LLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLI 182
Query: 497 FSQNIFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEI 318
FS+++F L D GSVE+KE +W+LL++GNSTNPADYFP +G LDL GQR+ VA+ LQ+I
Sbjct: 183 FSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQI 242
Query: 317 YGVWGPILTEKKLAKQHNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEW 138
Y VWG +L E++ K + + NDFVD+LL++GL+DQ+INALLME+FGAGTETSASTIEW
Sbjct: 243 YDVWGVMLKERRGTK--GSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW 300
Query: 137 ALAELIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
A+ EL K P + + IR EL++V+G TVKESD+P+LPYLQAFVKE
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKE 345
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 436 bits (1120), Expect = e-119
Identities = 218/345 (63%), Positives = 272/345 (78%), Gaps = 5/345 (1%)
Frame = -2
Query: 1022 LIAIVITSLFYLIF-----RDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAQLAGTYGD 858
+++I I S +L+F RD PIVGNLLQLG+KP ++FA+LA YG
Sbjct: 3 VVSIAIVSFSFLLFLFFILRDPRQKNLPPGPRPLPIVGNLLQLGDKPRAEFAKLAQKYGQ 62
Query: 857 LFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWK 678
LF+LKLG+QTVVVAS+P AA+E+LKTHD++LSGRYVFQSFR+K HVEN +VWS+CN+ WK
Sbjct: 63 LFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWK 122
Query: 677 SLRKVCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLI 498
LRKVCRTELFT KMIESQ+++RE KA E+V++LR +G VKIVEV+F TLVNIFGNLI
Sbjct: 123 LLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLI 182
Query: 497 FSQNIFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEI 318
FS+++F L D GSVE+KE +W+LLE+GNSTNPADYFP +G DL GQR+ VAD LQ+I
Sbjct: 183 FSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQI 242
Query: 317 YGVWGPILTEKKLAKQHNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEW 138
Y VWG L E++ K +N NDFVD+LL++GL DQ+INALLME+FG GTETSASTIEW
Sbjct: 243 YDVWGINLKERRGMKGSESN--NDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEW 300
Query: 137 ALAELIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
A+ EL K P + + IR EL++V+G TVKESD+P+LPYLQAFVKE
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKE 345
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 422 bits (1085), Expect = e-115
Identities = 209/342 (61%), Positives = 269/342 (78%), Gaps = 1/342 (0%)
Frame = -2
Query: 1025 ALIAIVITSLF-YLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAQLAGTYGDLFT 849
A++++ LF + I DS PIVGNLLQLGEKPH++FA+LA YG+LFT
Sbjct: 7 AMVSLFFIFLFIFFILCDSRNKDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEKYGELFT 66
Query: 848 LKLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWKSLR 669
LKLG+QTVVVAS+P AA+EILKT D++LSGRYVFQSFR+ HV N +VWS+CNE WK LR
Sbjct: 67 LKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNENWKLLR 126
Query: 668 KVCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLIFSQ 489
KVCRTELF+ KMIESQA +RE KA+++V +LRK + EVKIVEV+F TLVNIFGNLIFS+
Sbjct: 127 KVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLIFSK 186
Query: 488 NIFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEIYGV 309
++F L D GS E+KE +W+LL++GNSTNPADYFP +G DL GQR++VA L++IY V
Sbjct: 187 DVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVLKQIYDV 246
Query: 308 WGPILTEKKLAKQHNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEWALA 129
WG +L E++ H NDF ++LL++GL+DQ+INALLME+FGAGTET+ASTIEWA+
Sbjct: 247 WGVMLKERRSITGHR---ENDFANVLLNAGLDDQKINALLMELFGAGTETTASTIEWAIT 303
Query: 128 ELIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
EL N + + +R+EL++V+G +TVKES+IP+LPYLQA VKE
Sbjct: 304 ELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKE 345
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 276 bits (707), Expect = 1e-71
Identities = 139/313 (44%), Positives = 203/313 (64%), Gaps = 3/313 (0%)
Frame = -2
Query: 932 IVGNLLQLGEKPHSQFAQLAGTYGDLFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRY 753
I+GNLLQ+G+ PH++ A LA +G L +L+LGTQ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 587 IIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRY 646
Query: 752 VFQSFRIKNHVEN---CMVWSDCNETWKSLRKVCRTELFTRKMIESQAQVRECKAMELVE 582
+ S +KN N + DCN WK+LR +CR ELF+ K +ESQ ++RE K ELVE
Sbjct: 647 LSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVE 706
Query: 581 YLRKHQGNEVKIVEVIFATLVNIFGNLIFSQNIFKLSDENSGSVEMKEQIWRLLELGNST 402
+L +G VK+++++F T+ NI N FS ++ DE +K+ I + E G +
Sbjct: 707 FLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATP 766
Query: 401 NPADYFPFLGSLDLLGQRKDVADSLQEIYGVWGPILTEKKLAKQHNTNTTNDFVDLLLDS 222
N +DY+P LG LD+ G + + + I W IL E++ ++ N ++ DF++ LL+
Sbjct: 767 NLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEI 825
Query: 221 GLNDQQINALLMEIFGAGTETSASTIEWALAELIKNPELTANIRSELLSVLGKRTVKESD 42
G D QIN +++E+F AG ETS+ T+EWA+AELI+N + +R EL ++G+ V+ES
Sbjct: 826 GFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESH 885
Query: 41 IPNLPYLQAFVKE 3
+P LPYLQA VKE
Sbjct: 886 LPRLPYLQACVKE 898
Score = 264 bits (675), Expect = 8e-68
Identities = 137/315 (43%), Positives = 202/315 (64%), Gaps = 5/315 (1%)
Frame = -2
Query: 932 IVGNLLQLGEKPHSQFAQLAGTYGDLFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRY 753
I+GNLLQLG+ PH + A LA +G L +L+LGTQ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 1092 IIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRY 1151
Query: 752 VFQSFRIK----NHVENCMVWSDCNETWKSLRKVCRTELFTRKMIESQAQVRECKAMELV 585
V S +K NH+ +C WK+LR +CRTE+F+ K +ES ++RE K MELV
Sbjct: 1152 VSSSLSVKDPKLNHLSLAFA-KECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELV 1210
Query: 584 EYLRKHQGNEVKIVEVIFATLVNIFGNLIFSQNIFKLSDENSGSVEMKEQIWRLLELGNS 405
E+L +G VK+++++F T+ NI N FS ++ E +K+ I+R ELG +
Sbjct: 1211 EFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELGAT 1265
Query: 404 TNPADYFPFLGSLDLLGQRKDVADSLQEIYGVWGPILTEKKLAKQHNTNTTN-DFVDLLL 228
N +D++P L L+L G +K ++L I W L E++ KQ N +++ D ++ L
Sbjct: 1266 PNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEAFL 1323
Query: 227 DSGLNDQQINALLMEIFGAGTETSASTIEWALAELIKNPELTANIRSELLSVLGKRTVKE 48
+ D QIN +++E+F AG +TS TIEWA+ +LI+NP++ +R EL ++G+ V+E
Sbjct: 1324 EIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRE 1383
Query: 47 SDIPNLPYLQAFVKE 3
S +P+LPYLQA VKE
Sbjct: 1384 SHLPHLPYLQACVKE 1398
Score = 255 bits (652), Expect = 4e-65
Identities = 135/313 (43%), Positives = 202/313 (64%), Gaps = 3/313 (0%)
Frame = -2
Query: 932 IVGNLLQLGEKPHSQFAQLAGTYGDLFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRY 753
I+GNLLQ+G H + A LA +G L +L+LGTQ +VVAS+ AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGGNLHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGRY 106
Query: 752 VFQSFRIKNHVENCMVWSD---CNETWKSLRKVCRTELFTRKMIESQAQVRECKAMELVE 582
V + + + N + + CN W++L+ +CR ELF+ K +ES+ ++RE K MELVE
Sbjct: 107 VSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVMELVE 166
Query: 581 YLRKHQGNEVKIVEVIFATLVNIFGNLIFSQNIFKLSDENSGSVEMKEQIWRLLELGNST 402
+L K +G VK++++++ T+ NI N FS + + V +K+ + ELG +T
Sbjct: 167 FLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEGRDVRGVLLKDLLNENAELG-AT 225
Query: 401 NPADYFPFLGSLDLLGQRKDVADSLQEIYGVWGPILTEKKLAKQHNTNTTNDFVDLLLDS 222
N D++P LG LD+ G RK + + + I W IL E++ + H ++ DF+D LL++
Sbjct: 226 NILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRKQRIHGSSH-GDFLDALLET 284
Query: 221 GLNDQQINALLMEIFGAGTETSASTIEWALAELIKNPELTANIRSELLSVLGKRTVKESD 42
G D QIN ++ME+F AG ETS+ T+EWA+AELIKN + + +EL ++G+ V+ES
Sbjct: 285 GFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQIIGESPVRESH 344
Query: 41 IPNLPYLQAFVKE 3
+P+LPYLQA VKE
Sbjct: 345 LPHLPYLQACVKE 357
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 276 bits (707), Expect = 1e-71
Identities = 139/313 (44%), Positives = 203/313 (64%), Gaps = 3/313 (0%)
Frame = -2
Query: 932 IVGNLLQLGEKPHSQFAQLAGTYGDLFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRY 753
I+GNLLQ+G+ PH++ A LA +G L +L+LGTQ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRY 106
Query: 752 VFQSFRIKNHVEN---CMVWSDCNETWKSLRKVCRTELFTRKMIESQAQVRECKAMELVE 582
+ S +KN N + DCN WK+LR +CR ELF+ K +ESQ ++RE K ELVE
Sbjct: 107 LSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVE 166
Query: 581 YLRKHQGNEVKIVEVIFATLVNIFGNLIFSQNIFKLSDENSGSVEMKEQIWRLLELGNST 402
+L +G VK+++++F T+ NI N FS ++ DE +K+ I + E G +
Sbjct: 167 FLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATP 226
Query: 401 NPADYFPFLGSLDLLGQRKDVADSLQEIYGVWGPILTEKKLAKQHNTNTTNDFVDLLLDS 222
N +DY+P LG LD+ G + + + I W IL E++ ++ N ++ DF++ LL+
Sbjct: 227 NLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEI 285
Query: 221 GLNDQQINALLMEIFGAGTETSASTIEWALAELIKNPELTANIRSELLSVLGKRTVKESD 42
G D QIN +++E+F AG ETS+ T+EWA+AELI+N + +R EL ++G+ V+ES
Sbjct: 286 GFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESH 345
Query: 41 IPNLPYLQAFVKE 3
+P LPYLQA VKE
Sbjct: 346 LPRLPYLQACVKE 358
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 276 bits (706), Expect = 2e-71
Identities = 139/313 (44%), Positives = 203/313 (64%), Gaps = 3/313 (0%)
Frame = -2
Query: 932 IVGNLLQLGEKPHSQFAQLAGTYGDLFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRY 753
I+GNLLQ+G+ PH++ A LA +G L +L+LGTQ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRY 106
Query: 752 VFQSFRIKNHVEN---CMVWSDCNETWKSLRKVCRTELFTRKMIESQAQVRECKAMELVE 582
+ S +KN N + DCN WK+LR +CR ELF+ K +ESQ ++RE K ELVE
Sbjct: 107 LSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVE 166
Query: 581 YLRKHQGNEVKIVEVIFATLVNIFGNLIFSQNIFKLSDENSGSVEMKEQIWRLLELGNST 402
+L +G VK+++++F T+ NI N FS ++ DE +K+ I + E G +
Sbjct: 167 FLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATP 226
Query: 401 NPADYFPFLGSLDLLGQRKDVADSLQEIYGVWGPILTEKKLAKQHNTNTTNDFVDLLLDS 222
N +DY+P LG LD+ G + + + I W IL E++ ++ N ++ DF++ LL+
Sbjct: 227 NLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEI 285
Query: 221 GLNDQQINALLMEIFGAGTETSASTIEWALAELIKNPELTANIRSELLSVLGKRTVKESD 42
G D QIN +++E+F AG ETS+ T+EWA+AELI+N + +R EL ++G+ V+ES
Sbjct: 286 GFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESH 345
Query: 41 IPNLPYLQAFVKE 3
+P LPYLQA VKE
Sbjct: 346 LPRLPYLQACVKE 358
>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 275 bits (703), Expect = 4e-71
Identities = 150/344 (43%), Positives = 215/344 (62%), Gaps = 3/344 (0%)
Frame = -2
Query: 1025 ALIAIVITSLFYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKP---HSQFAQLAGTYGDL 855
AL+ ++ T L Y++F+ S I+GN+ L H +A T+G +
Sbjct: 6 ALLYLIPTILLYILFKPRSKNLPPGPKPWP-IIGNIPALFSSAYPMHVALTNMARTHGPI 64
Query: 854 FTLKLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWKS 675
L LGTQ VAST AA EILKTHDR SGR V SFR+K+H++ +VW+DC+E WK
Sbjct: 65 MILWLGTQPTAVASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADCSEYWKL 124
Query: 674 LRKVCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLIF 495
LRK+ RTE+F+ KM++ Q VRE K ELVE+L+ +G VKI +++F TL+NI GN+IF
Sbjct: 125 LRKIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIF 184
Query: 494 SQNIFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQEIY 315
S+++F+ + +M+ I +L +G + N A+++P DL G +K E+
Sbjct: 185 SKDVFRYVGADGD--KMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVM 242
Query: 314 GVWGPILTEKKLAKQHNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIEWA 135
+W + E+K+ + D +D+LL + ND QINA+ +E FG G+ETS++T EWA
Sbjct: 243 KMWKGTVEERKVKRNEE---PKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWA 299
Query: 134 LAELIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
LAELIKNP + A +R EL V+GK TVKESD+P+LPYLQA +KE
Sbjct: 300 LAELIKNPHILAKVRQELDQVVGKSTVKESDLPHLPYLQACIKE 343
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 273 bits (698), Expect = 2e-70
Identities = 147/348 (42%), Positives = 224/348 (64%), Gaps = 9/348 (2%)
Frame = -2
Query: 1019 IAIVITSLFYLI-------FRDSSXXXXXXXXXXXPIVGNLL-QLGEKPHSQFAQLAGTY 864
+A++++ +F+L+ F S PI+G LL +L ++PH + ++LA +
Sbjct: 1 MALLVSFIFFLLSILSVVLFLKPSPNNLPPGPFSWPIIGTLLPKLKKQPHVELSKLAQRF 60
Query: 863 GDLFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNET 684
G L LK G + VVVAS+ +AA E+LK DR+LSGR+ S RIK ++E+ MVW+DC +
Sbjct: 61 GPLMLLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDY 120
Query: 683 WKSLRKVCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGN 504
WK +RKV RTELF+ KM++ QA RE K EL+++L + +G +V +VIF +++NI G
Sbjct: 121 WKMVRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGA 180
Query: 503 LIFSQNIFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQ 324
LIFS++++ D ++ MK I +L+ L N AD +P LG D G RK A ++
Sbjct: 181 LIFSKDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVK 240
Query: 323 EIYGVWGPILTEKKLAKQHNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTI 144
+ W I+ ++ ++++ ++ NDF+ +LLDSG +D QI+A+L+E FG G++TS STI
Sbjct: 241 RMNESWAAIVKQR---RKNDDHSKNDFLQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTI 297
Query: 143 EWALAELIKNPELTANIRSELLSVLGK-RTVKESDIPNLPYLQAFVKE 3
EWA+AEL++NPE +R EL V+ + VKESD+PNLPYL A VKE
Sbjct: 298 EWAMAELLRNPEKLVKVREELDRVIRRSNNVKESDLPNLPYLHACVKE 345
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 271 bits (694), Expect = 5e-70
Identities = 146/346 (42%), Positives = 217/346 (62%), Gaps = 5/346 (1%)
Frame = -2
Query: 1025 ALIAIVITSLFYLIFRDSSXXXXXXXXXXXPIVGNLLQL---GEKP-HSQFAQLAGTYGD 858
AL +++ L +++ P++GNL L E P H A +A T+G
Sbjct: 6 ALFSLIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGP 65
Query: 857 LFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNHVENCMVWSDCNETWK 678
+ L LGTQ V+AST AA EILKTHDR+ S R++ SFR+K+H++ +VWSDC + WK
Sbjct: 66 IMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWK 125
Query: 677 SLRKVCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIVEVIFATLVNIFGNLI 498
LRK+ RTE+F+ KM+++Q+ VRE K EL+++LR +G VKI + +F TL+NI GN++
Sbjct: 126 LLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVV 185
Query: 497 FSQNIFKLSDE-NSGSVEMKEQIWRLLELGNSTNPADYFPFLGSLDLLGQRKDVADSLQE 321
FS+++F DE + G ++ I +L +G N A+++P L LDL G +K +
Sbjct: 186 FSKDVFVYGDETDKGGIQ--NLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIR 243
Query: 320 IYGVWGPILTEKKLAKQHNTNTTNDFVDLLLDSGLNDQQINALLMEIFGAGTETSASTIE 141
+ +W + E+ K + + D +D+LL + ND QINAL +E FG G+ETS++TIE
Sbjct: 244 VMKMWEGTVKER---KANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 300
Query: 140 WALAELIKNPELTANIRSELLSVLGKRTVKESDIPNLPYLQAFVKE 3
W +AELIK+P+ A +R EL V+G T+KESD+P LPYLQA +KE
Sbjct: 301 WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKE 346
>ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 266 bits (679), Expect = 3e-68
Identities = 137/302 (45%), Positives = 201/302 (66%), Gaps = 2/302 (0%)
Frame = -2
Query: 902 KPHSQFAQLAGTYGDLFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRYVFQSFRIKNH 723
KP+ + +LA YG L + G + VVVAS +AA E+LK DRVLSGR+ S R+K +
Sbjct: 48 KPNLELFKLAQRYGPLMLFRFGFENVVVASNHVAAMEVLKNQDRVLSGRFKANSVRVKGY 107
Query: 722 VENCMVWSDCNETWKSLRKVCRTELFTRKMIESQAQVRECKAMELVEYLRKHQGNEVKIV 543
+E MVW+DC + WK +RK+ RTELF+ KM++ A VRE K EL+++LR+ +G EV V
Sbjct: 108 IEYSMVWADCTDYWKMVRKILRTELFSTKMLDVHAHVREEKVSELMKFLRRKEGEEVNFV 167
Query: 542 EVIFATLVNIFGNLIFSQNIFKLSDENSGSVEMKEQIWRLLELGNSTNPADYFP-FLGSL 366
+VIF ++N+ G LI+S++++ D+ ++ MK I +L+ L + N AD +P F
Sbjct: 168 DVIFGCILNMLGALIYSKDVYDFEDKTDINLGMKGMIRQLMILAATPNIADLYPIFFDGS 227
Query: 365 DLLGQRKDVADSLQEIYGVWGPILTEKKLAKQHNTNTTNDFVDLLLDSGLNDQQINALLM 186
D G RK+ A ++ + W I+ E+ +++N +T ND + +LLDSG +D QI+A+ +
Sbjct: 228 DFQGLRKESAACVKRMSESWAAIINER---RKNNDHTKNDLLQVLLDSGFSDPQIDAIFL 284
Query: 185 EIFGAGTETSASTIEWALAELIKNPELTANIRSELLSVLGK-RTVKESDIPNLPYLQAFV 9
E FG G++TSASTIEWALAEL++NPE + EL V+G+ TVK+SD+PNLPYL A V
Sbjct: 285 ETFGPGSDTSASTIEWALAELLRNPEKLVKLHEELDRVIGRNNTVKDSDLPNLPYLHACV 344
Query: 8 KE 3
KE
Sbjct: 345 KE 346
>ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 493
Score = 264 bits (675), Expect = 8e-68
Identities = 137/315 (43%), Positives = 202/315 (64%), Gaps = 5/315 (1%)
Frame = -2
Query: 932 IVGNLLQLGEKPHSQFAQLAGTYGDLFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRY 753
I+GNLLQLG+ PH + A LA +G L +L+LGTQ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRY 106
Query: 752 VFQSFRIK----NHVENCMVWSDCNETWKSLRKVCRTELFTRKMIESQAQVRECKAMELV 585
V S +K NH+ +C WK+LR +CRTE+F+ K +ES ++RE K MELV
Sbjct: 107 VSSSLSVKDPKLNHLSLAFA-KECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELV 165
Query: 584 EYLRKHQGNEVKIVEVIFATLVNIFGNLIFSQNIFKLSDENSGSVEMKEQIWRLLELGNS 405
E+L +G VK+++++F T+ NI N FS ++ E +K+ I+R ELG +
Sbjct: 166 EFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELGAT 220
Query: 404 TNPADYFPFLGSLDLLGQRKDVADSLQEIYGVWGPILTEKKLAKQHNTNTTN-DFVDLLL 228
N +D++P L L+L G +K ++L I W L E++ KQ N +++ D ++ L
Sbjct: 221 PNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEAFL 278
Query: 227 DSGLNDQQINALLMEIFGAGTETSASTIEWALAELIKNPELTANIRSELLSVLGKRTVKE 48
+ D QIN +++E+F AG +TS TIEWA+ +LI+NP++ +R EL ++G+ V+E
Sbjct: 279 EIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRE 338
Query: 47 SDIPNLPYLQAFVKE 3
S +P+LPYLQA VKE
Sbjct: 339 SHLPHLPYLQACVKE 353
>ref|XP_009610421.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nicotiana tomentosiformis]
Length = 489
Score = 263 bits (672), Expect = 2e-67
Identities = 142/315 (45%), Positives = 209/315 (66%), Gaps = 5/315 (1%)
Frame = -2
Query: 932 IVGNLLQLGEKPHSQFAQLAGTYGDLFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRY 753
I+GN+LQLG+ PH Q A +G L L+LGTQ VV+AS+P +A+EILKT DR+LS R
Sbjct: 39 ILGNILQLGKNPHISMTQFAKIHGPLICLRLGTQLVVIASSPDSAAEILKTQDRLLSARS 98
Query: 752 VFQSFRIKNHV--ENCMVWS-DCNETWKSLRKVCRTELFTRKMIESQAQVRECKAMELVE 582
V + K V ++ +VWS D WKSLR CRT LF+ K +ESQA +RE K E++E
Sbjct: 99 VPRVAPYKLSVIDQHSIVWSSDLTNHWKSLRAFCRTHLFSAKAVESQADLREKKVFEMIE 158
Query: 581 YLRKHQGNEVKIVEVIFATLVNIFGNLIFSQNIFKLSDENSGSVEMKEQIWRLLELGNST 402
+LR ++G VKI E++FAT++N GNL F++N+ L E + S +K I R +ELG
Sbjct: 159 FLRSNKGKTVKISEILFATILNTLGNLFFTKNLCDLDSEGNTS-GIKHVIRRFVELGAMP 217
Query: 401 NPADYFPFLGSLDLLGQRKDVADSLQEIYGVWGPILTEKKLA-KQHNTNTTNDFVDLLLD 225
N ++++PFL +LDL G RK ++ +W ++ EK+ A ++N DF+D+++D
Sbjct: 218 NISEFYPFLDALDLQGLRKLTNIYQDQLISIWAEVVKEKRQAISCGSSNKNQDFLDIMID 277
Query: 224 SGLNDQQINALLMEIFGAGTETSASTIEWALAELIKNPELTANIRSELLSVLGKR-TVKE 48
SG +D QIN +LME+ AGT+T+ STIEWA+AEL++N ++ +++E+ S +G ++E
Sbjct: 278 SGFSDLQINIILMELIAAGTDTTTSTIEWAMAELLRNKKVMHKLQAEIRSKIGSNANIRE 337
Query: 47 SDIPNLPYLQAFVKE 3
S+I LPYL A VKE
Sbjct: 338 SNISELPYLAACVKE 352
>ref|XP_009757123.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
isoform X1 [Nicotiana sylvestris]
gi|698520618|ref|XP_009757124.1| PREDICTED: probable
(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 isoform
X2 [Nicotiana sylvestris]
Length = 489
Score = 261 bits (668), Expect = 5e-67
Identities = 142/315 (45%), Positives = 210/315 (66%), Gaps = 5/315 (1%)
Frame = -2
Query: 932 IVGNLLQLGEKPHSQFAQLAGTYGDLFTLKLGTQTVVVASTPLAASEILKTHDRVLSGRY 753
I+GN+LQLG+ PH Q A +G L +L+LGTQ VV+AS+P +A+EILKT DR+LS R
Sbjct: 39 ILGNILQLGKNPHISMTQFAKIHGPLISLRLGTQLVVIASSPDSAAEILKTQDRLLSARS 98
Query: 752 VFQSFRIKNHV--ENCMVWS-DCNETWKSLRKVCRTELFTRKMIESQAQVRECKAMELVE 582
V + K V ++ +VWS D + WKSLR CRT LF+ K +ESQA +RE K E++E
Sbjct: 99 VPRVAPYKLSVVDQHSLVWSSDLSNHWKSLRAFCRTHLFSAKAVESQAALREKKVFEMIE 158
Query: 581 YLRKHQGNEVKIVEVIFATLVNIFGNLIFSQNIFKLSDENSGSVEMKEQIWRLLELGNST 402
+LR +G VKI E++FAT++N GNL F++++ L E + S +K I + +ELG
Sbjct: 159 FLRSKKGKTVKISEIMFATILNTLGNLFFTKDLCDLDYEGNTS-GIKHVIRKFVELGAMP 217
Query: 401 NPADYFPFLGSLDLLGQRKDVADSLQEIYGVWGPILTEKKLA-KQHNTNTTNDFVDLLLD 225
N ++++PFL +LDL G RK +++ +W I+ EK+ A + ++N DF+D+++
Sbjct: 218 NISEFYPFLDALDLQGLRKQTKIYQGQLFNIWAEIVKEKRQAISRGSSNKIQDFLDIMIY 277
Query: 224 SGLNDQQINALLMEIFGAGTETSASTIEWALAELIKNPELTANIRSELLSVLGKRT-VKE 48
SG D QIN +LME+ AGT+T+ STIEWA+AEL++N E+ +++EL S +G ++E
Sbjct: 278 SGFTDLQINIILMELIAAGTDTTTSTIEWAMAELLRNKEVMHKLQAELRSKIGSNAIIRE 337
Query: 47 SDIPNLPYLQAFVKE 3
S+I LPYL A VKE
Sbjct: 338 SNISELPYLAACVKE 352