BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver31_contig00015368
         (1826 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_010243867.1| PREDICTED: kinetochore protein nuf2 [Nelumbo...   465   e-128
ref|XP_002283312.1| PREDICTED: probable kinetochore protein NUF2...   427   e-116
ref|XP_006342187.1| PREDICTED: putative leucine-rich repeat-cont...   422   e-115
ref|XP_004248545.1| PREDICTED: probable kinetochore protein NUF2...   421   e-115
ref|XP_002269064.1| PREDICTED: kinetochore protein nuf2 [Vitis v...   421   e-115
emb|CAN82250.1| hypothetical protein VITISV_036492 [Vitis vinifera]   416   e-113
ref|XP_008218786.1| PREDICTED: probable kinetochore protein nuf2...   410   e-111
ref|XP_006446114.1| hypothetical protein CICLE_v10015244mg [Citr...   405   e-110
ref|XP_012071329.1| PREDICTED: probable kinetochore protein NUF2...   402   e-109
ref|XP_007029773.1| Kinetochore protein nuf2 isoform 1 [Theobrom...   402   e-109
ref|XP_012449504.1| PREDICTED: probable kinetochore protein NUF2...   401   e-109
ref|XP_012445609.1| PREDICTED: probable kinetochore protein NUF2...   399   e-108
ref|XP_011023065.1| PREDICTED: kinetochore protein nuf2 [Populus...   397   e-107
ref|XP_009355229.1| PREDICTED: probable kinetochore protein NUF2...   397   e-107
ref|XP_011087745.1| PREDICTED: probable kinetochore protein NUF2...   397   e-107
ref|XP_012071330.1| PREDICTED: probable kinetochore protein NUF2...   396   e-107
ref|XP_009804688.1| PREDICTED: kinetochore protein NDC80 homolog...   395   e-107
ref|XP_002309861.2| hypothetical protein POPTR_0007s03160g [Popu...   395   e-107
ref|XP_009622956.1| PREDICTED: huntingtin-interacting protein 1-...   393   e-106
gb|KJB68067.1| hypothetical protein B456_010G224800 [Gossypium r...   389   e-105

>ref|XP_010243867.1| PREDICTED: kinetochore protein nuf2 [Nelumbo nucifera]
          Length = 440

 Score =  465 bits (1197), Expect = e-128
 Identities = 249/437 (56%), Positives = 316/437 (72%)
 Frame = -1

Query: 1754 MASTFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQDDHRQ 1575
            MAS+FS  ++PRKDIV IL E QIA+++EE L+NP SD+V ++Y N+L+Y+DPLQ+DH Q
Sbjct: 1    MASSFSADLIPRKDIVQILSEYQIANLREEDLINPNSDLVTALYMNILVYIDPLQEDHGQ 60

Query: 1574 ADFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSA 1395
             DF+AL QLENPD H DSVR  NL RK+ E++ +VSCP +F+ RDL KPDTHR   FLSA
Sbjct: 61   VDFSALEQLENPDYHVDSVRASNLFRKMTELVDAVSCPINFIFRDLVKPDTHRTTAFLSA 120

Query: 1394 ILNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEV 1215
            ILNF L+R TKL  +Q   DQVN  E +R ELE++IS+ +ME+    EA EREQP + EV
Sbjct: 121  ILNFFLYRTTKLNDIQKVFDQVNFDEVRRQELETQISQKNMEILEHREAREREQPLVLEV 180

Query: 1214 DGKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRI 1035
            D +VKELRQ IQ LNNHQM +KTS   ++ +  EIDEKIS+AEF L+QSAQENAKL S+I
Sbjct: 181  DAQVKELRQNIQGLNNHQMSLKTSFRTLREKTKEIDEKISNAEFLLLQSAQENAKLQSKI 240

Query: 1034 VQSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTK 855
            VQSP+KLQ  LEEKKSI+ +VKNSERSAMQSFQEK+ T++VYSKA KKMSK   QM   +
Sbjct: 241  VQSPEKLQRALEEKKSIQAEVKNSERSAMQSFQEKTSTVEVYSKAHKKMSKNFTQMQEIQ 300

Query: 854  EQADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHE 675
            EQ + +KT++KDVKV+K K +++    MSLEAK VERQ KV             +++K E
Sbjct: 301  EQVNKSKTIDKDVKVIKFKFSEDEESIMSLEAKLVERQRKVEQQESSRKALEKERDLKRE 360

Query: 674  EVTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCE 495
            EV K L+         K D   R+ K+E +VAEGD I  + N   E+ AA + +L  KCE
Sbjct: 361  EVNKELNHVILDVEAKKHDLQERQRKIEVMVAEGDDITLKINSAKETSAAMQQKLANKCE 420

Query: 494  EIVNEFHSYSQSIGSYL 444
            EIVNEFH+YS SIG++L
Sbjct: 421  EIVNEFHNYSNSIGAHL 437


>ref|XP_002283312.1| PREDICTED: probable kinetochore protein NUF2 [Vitis vinifera]
            gi|296082133|emb|CBI21138.3| unnamed protein product
            [Vitis vinifera]
          Length = 453

 Score =  427 bits (1098), Expect = e-116
 Identities = 229/439 (52%), Positives = 305/439 (69%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQDDHRQAD 1569
            S F +P+LPR DI+AIL+ES+IA++ E++L+NP  D V  +Y+ LLI+LD LQ+D  Q +
Sbjct: 2    SKFEYPMLPRSDIIAILNESKIATVYEDNLINPNPDFVSDLYTRLLIHLDSLQEDPGQIE 61

Query: 1568 FNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAIL 1389
            F AL QLENPD H DSVR++NL  KI E++AS+ CP  F L+DL +PD  R   FL  IL
Sbjct: 62   FAALEQLENPDWHRDSVRVMNLYSKIKELVASLECPKKFTLKDLIRPDRDRTERFLGTIL 121

Query: 1388 NFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVDG 1209
            NF LH+++K+  L+P V+++ + +EQRN+ + RIS+L  E+++ +EA ERE+P + EV+ 
Sbjct: 122  NFFLHKDSKMNDLRPIVEELTLLDEQRNQFKDRISQLDAEIADCSEARERERPLVEEVEV 181

Query: 1208 KVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIVQ 1029
            KVKELRQTI  LNN QM ++ S   +K + TE+DEKISSAEF L+QS QENA L S+I+Q
Sbjct: 182  KVKELRQTIPSLNNQQMSLRASFRKMKEKVTEMDEKISSAEFALVQSVQENANLRSKIIQ 241

Query: 1028 SPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKEQ 849
            SPDKLQ  LEEKKS R +VKN+E+SAMQSFQEKS  L+VY+KA KKMSK  AQM   +EQ
Sbjct: 242  SPDKLQRALEEKKSDRIEVKNAEKSAMQSFQEKSAILEVYTKAGKKMSKHFAQMQTIQEQ 301

Query: 848  ADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEEV 669
             ++AK +EKD+KV+K K ++EG+L  SLEAK VE QGKV             ++ K EE 
Sbjct: 302  VNSAKAIEKDLKVIKTKLSEEGVLEKSLEAKLVEWQGKVEQLDDLRKQLEKERDFKCEEA 361

Query: 668  TKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEEI 489
             K L+         + +  AR+ KVEA  AE DGI  + N   ESGAA + +L  K EEI
Sbjct: 362  AKELNNVKLAVESSRHELEARQKKVEAQAAEVDGITLKINSVKESGAAKQQELISKGEEI 421

Query: 488  VNEFHSYSQSIGSYLDGVQ 432
            + EFH Y+ SIG  L  +Q
Sbjct: 422  MKEFHLYANSIGVLLPRIQ 440


>ref|XP_006342187.1| PREDICTED: putative leucine-rich repeat-containing protein
            DDB_G0290503-like [Solanum tuberosum]
          Length = 450

 Score =  422 bits (1085), Expect = e-115
 Identities = 217/439 (49%), Positives = 297/439 (67%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQDDHRQAD 1569
            S F +P LPR+DI+A+L E+QIAS+ +E L+ PT D V  +YS++L+++D LQDDH Q D
Sbjct: 2    SRFDYPTLPRQDIIAVLAEAQIASVSDEDLIKPTPDFVTKLYSSILLHIDTLQDDHDQVD 61

Query: 1568 FNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAIL 1389
            F+AL  LENPDLH DS R +NL  KI +++A++ CP  F LRDL KPD  R   F+ AIL
Sbjct: 62   FSALEHLENPDLHVDSFRTINLFHKIRDMLAALDCPEIFTLRDLIKPDPDRTRFFVGAIL 121

Query: 1388 NFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVDG 1209
            NFCLHR+TKL  ++P V+ + + +EQR  LE+RIS+L+ E++  NE+ ERE P + E+D 
Sbjct: 122  NFCLHRDTKLNAIRPIVEHLTLIDEQRLALEARISQLNEEIAVHNESREREMPLVQEIDS 181

Query: 1208 KVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIVQ 1029
             VKELRQTI  LNNHQM +K S+  +K RA EIDEKIS+A+F L+Q+  +NA L S+IVQ
Sbjct: 182  NVKELRQTISGLNNHQMSLKASIRKLKERAKEIDEKISNADFALVQAVHDNANLRSKIVQ 241

Query: 1028 SPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKEQ 849
            SPDKLQ  LEEKKS + +++N+ER+AMQSFQ K+  L+VY+KA KKMSK   QM   +EQ
Sbjct: 242  SPDKLQRALEEKKSFQAEIRNAERAAMQSFQNKTAILEVYTKAYKKMSKNFNQMQAIQEQ 301

Query: 848  ADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEEV 669
             ++ K++EKDVKVLK K +DE +   SLEAK VERQGK              +N+  EE 
Sbjct: 302  VNSTKSIEKDVKVLKLKLSDEEVQEKSLEAKLVERQGKADQLEELRKQLEKERNLSFEEA 361

Query: 668  TKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEEI 489
             K L          +    AR+  +E ++AE D I ++ N   ESGA+   +L + CEE+
Sbjct: 362  AKELKNVKLEVESKRHGLEARQNDLEGVLAEADAITEKINSVRESGASKCQELDRNCEEV 421

Query: 488  VNEFHSYSQSIGSYLDGVQ 432
            + EF+ +S SI   L  ++
Sbjct: 422  IAEFYRHSNSIKDLLPDIE 440


>ref|XP_004248545.1| PREDICTED: probable kinetochore protein NUF2 [Solanum lycopersicum]
          Length = 453

 Score =  421 bits (1083), Expect = e-115
 Identities = 217/439 (49%), Positives = 296/439 (67%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQDDHRQAD 1569
            S F +P LPR+DI+A+L E+QIAS+ +E L+ PT D V  +YS++L+++D LQDDH Q D
Sbjct: 2    SRFDYPTLPRQDIIAVLGEAQIASVSDEDLIKPTPDFVTKLYSSILLHIDTLQDDHDQVD 61

Query: 1568 FNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAIL 1389
            F+AL  LENPDLH DS R +NL  KI  +++++ CP  F LRDL KPD  R   F+ AIL
Sbjct: 62   FSALEYLENPDLHVDSFRTINLFHKIRNMLSALGCPEIFTLRDLIKPDPDRTRFFVGAIL 121

Query: 1388 NFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVDG 1209
            NFCLHR+TKL  ++P V+ + + +EQR  LE+RIS+L+ E++  NE+ ERE P + E+D 
Sbjct: 122  NFCLHRDTKLNAIRPIVEDLTLIDEQRLALEARISQLNEEIAVQNESREREMPLVQEIDS 181

Query: 1208 KVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIVQ 1029
             VKELRQTI  LNNHQM +K S+  +K RA EIDEKIS+A+F L+Q+  +NA L S+IVQ
Sbjct: 182  NVKELRQTISGLNNHQMSLKASIRKLKERAKEIDEKISNADFALVQAVHDNANLRSKIVQ 241

Query: 1028 SPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKEQ 849
            SPDKLQ  LEEKKS + +++N+ER+AMQSFQ+K+  L+VY+KA KKMSK   QM   +EQ
Sbjct: 242  SPDKLQRALEEKKSFQAEIRNAERAAMQSFQDKTAILEVYTKAYKKMSKNFNQMQAIQEQ 301

Query: 848  ADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEEV 669
             ++ K++EKDVKVLK K +DE +   SLEAK VERQGK              +N+  EE 
Sbjct: 302  VNSTKSIEKDVKVLKLKLSDEEVQEKSLEAKLVERQGKADQLEELRKQLEKERNLSFEEA 361

Query: 668  TKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEEI 489
             K L          +    AR+  +E ++AE D I +R N   ESGA    +L + CEE+
Sbjct: 362  NKELKNVKLEVESKRHGLEARQNDLEGVLAEADAITERINSVRESGALKCQELDRNCEEV 421

Query: 488  VNEFHSYSQSIGSYLDGVQ 432
            + EF+ +S SI   L  ++
Sbjct: 422  IAEFYRHSSSIKDLLPDIE 440


>ref|XP_002269064.1| PREDICTED: kinetochore protein nuf2 [Vitis vinifera]
            gi|298204517|emb|CBI23792.3| unnamed protein product
            [Vitis vinifera]
          Length = 453

 Score =  421 bits (1083), Expect = e-115
 Identities = 225/439 (51%), Positives = 300/439 (68%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQDDHRQAD 1569
            S F +P++PR +I+AIL+ES+IA++ +E L+NP  D V  +Y+ LLI+LD LQ+D  Q +
Sbjct: 2    SKFEYPMIPRSEIIAILNESKIATVYDEDLINPNPDFVSDLYTRLLIHLDSLQEDPGQVE 61

Query: 1568 FNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAIL 1389
            F AL +LENPD H DSVR++NL  KI E++AS+ CP  F L+DL +PD  R   FL  IL
Sbjct: 62   FAALERLENPDWHRDSVRIMNLYSKIKELVASLDCPWKFTLKDLIRPDRDRTEKFLGTIL 121

Query: 1388 NFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVDG 1209
            NF LH+++K+  L+P V++ N+ +E+R  LE RIS+L  E+++ NEA ER  P + E++ 
Sbjct: 122  NFFLHKDSKMNELRPIVEESNLLDEERKLLEDRISQLDAEITDYNEARERAMPLVQEIEV 181

Query: 1208 KVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIVQ 1029
            KVK+LRQ I  LNN QM ++TS   +K +  E+DEKISSAEF L+QS QENA L S+IVQ
Sbjct: 182  KVKDLRQAIPSLNNQQMSLRTSFRKMKEKVAEMDEKISSAEFALVQSVQENANLRSKIVQ 241

Query: 1028 SPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKEQ 849
            SPDKLQ TLEEKKS+R + KN+ERSAMQSFQEKS  ++VY+KA KKMSK  AQM   +EQ
Sbjct: 242  SPDKLQRTLEEKKSVRVEAKNAERSAMQSFQEKSAIVEVYTKAGKKMSKHFAQMQAIQEQ 301

Query: 848  ADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEEV 669
             ++AK +EKD+KVLK K ++EG+L  S EAK +ERQ KV             +N K EE 
Sbjct: 302  VNSAKAIEKDLKVLKTKLSEEGVLDKSFEAKLLERQRKVEQLDELRKQLEKERNFKCEEA 361

Query: 668  TKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEEI 489
             K L+         +R+  AR+ KVEA  AE D    + N   ESGAA + +L +K EEI
Sbjct: 362  AKELNNVKLAVESKRRELEARQRKVEAEAAEVDATTLKINSVKESGAAKQQKLTRKGEEI 421

Query: 488  VNEFHSYSQSIGSYLDGVQ 432
            V EFH Y+ SIG  L  +Q
Sbjct: 422  VKEFHQYANSIGVLLPRIQ 440


>emb|CAN82250.1| hypothetical protein VITISV_036492 [Vitis vinifera]
          Length = 473

 Score =  416 bits (1068), Expect = e-113
 Identities = 229/459 (49%), Positives = 305/459 (66%), Gaps = 20/459 (4%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQDDHRQAD 1569
            S F +P+LPR DI+AIL+ES+IA++ E++L+NP  D V  +Y+ LLI+LD LQ+D  Q +
Sbjct: 2    SKFEYPMLPRSDIIAILNESKIATVYEDNLINPNPDFVSDLYTRLLIHLDSLQEDPGQIE 61

Query: 1568 FNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAIL 1389
            F AL QLENPD H DSVR++NL  KI E++AS+ CP  F L+DL +PD  R   FL  IL
Sbjct: 62   FAALEQLENPDWHRDSVRVMNLYSKIKELVASLECPKKFTLKDLIRPDRDRTERFLGTIL 121

Query: 1388 NFCLHR--------------------ETKLVLLQPFVDQVNIHEEQRNELESRISELHME 1269
            NF LH+                    ++K+  L+P V+++ + +EQRN+ + RIS+L  E
Sbjct: 122  NFFLHKKCKGISPKGPMTKTHKEETKDSKMNDLRPIVEELTLLDEQRNQFKDRISQLDAE 181

Query: 1268 MSNLNEANEREQPFIAEVDGKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSA 1089
            +++ +EA ERE+P + EV+ KVKELRQTI  LNN QM ++ S   +K + TE+DEKISSA
Sbjct: 182  IADCSEARERERPLVEEVEVKVKELRQTIPSLNNQQMSLRASFRKMKEKVTEMDEKISSA 241

Query: 1088 EFTLIQSAQENAKLLSRIVQSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVY 909
            EF L+QS QENA L S+I+QSPDKLQ  LEEKKS R +VKN+E+SAMQSFQEKS  L+VY
Sbjct: 242  EFALVQSVQENANLRSKIIQSPDKLQRALEEKKSDRIEVKNAEKSAMQSFQEKSAILEVY 301

Query: 908  SKALKKMSKQLAQMHGTKEQADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVX 729
            +KA KKMSK  AQM   +EQ ++AK +EKD+KV+K K ++EG+L  SLEAK VE QGKV 
Sbjct: 302  TKAGKKMSKHFAQMQTIQEQVNSAKAIEKDLKVIKTKLSEEGVLXKSLEAKLVEWQGKVE 361

Query: 728  XXXXXXXXXXXXKNMKHEEVTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNN 549
                        ++ K EE  K L+         + +  AR+ KVEA  AE DGI  + N
Sbjct: 362  QLDDLRKQLEKERDFKCEEAAKELNNVKLAVESSRHELEARQKKVEAQAAEVDGITLKIN 421

Query: 548  LEIESGAATKHQLYKKCEEIVNEFHSYSQSIGSYLDGVQ 432
               ESGAA + +L  K EEI+ EFH Y+ SIG  L  +Q
Sbjct: 422  SVKESGAAKQQELISKGEEIMKEFHLYANSIGVLLPRIQ 460


>ref|XP_008218786.1| PREDICTED: probable kinetochore protein nuf2 [Prunus mume]
          Length = 445

 Score =  410 bits (1055), Expect = e-111
 Identities = 223/432 (51%), Positives = 293/432 (67%), Gaps = 1/432 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPL-QDDHRQA 1572
            S F FP L R DIV IL +SQI +I +  LVNP  D V  +++ +L ++D L ++D+ Q 
Sbjct: 2    SKFEFPKLSRNDIVTILADSQIVAISDRDLVNPNPDFVADLFARILSHIDFLHEEDYEQV 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            +F AL QLENPDLH DS R + L  ++ EV+A V CP  F L+DL +P+T R   FLSAI
Sbjct: 62   EFAALEQLENPDLHIDSARSMKLYNRMKEVVALVDCPKRFTLKDLIRPETDRTEYFLSAI 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNFCLHRETK+ +L   VDQV   +EQRN  E RIS+L+ E+++ NEA E+E P + EVD
Sbjct: 122  LNFCLHRETKMNILTKVVDQVTDIDEQRNRWEDRISQLNAEIADYNEAREKELPLVQEVD 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
             KVKELRQTI  LNN QM ++TSL  +K +  E++EKISSAEF L+QS QE+A L SRIV
Sbjct: 182  AKVKELRQTIAGLNNQQMSLRTSLRKLKEKTGEMEEKISSAEFALVQSVQESANLRSRIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSPDKLQ  LEEKKS+RE+ KN ERSAMQSF+EK+   +VY+K  KK+SK LAQM   +E
Sbjct: 242  QSPDKLQRALEEKKSVREEAKNFERSAMQSFKEKTDVDEVYTKVSKKLSKHLAQMQAIQE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK+V++D K +KAK  D+G+L  SL+AK VER+GKV             +++K EE
Sbjct: 302  QVNSAKSVDRDFKAVKAKLADDGVLSRSLQAKLVEREGKVEQFNELKKQLEKERDLKFEE 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             +K L+         + D  AR+  VEA VAE D +  +     ESGA+ + QL +KCEE
Sbjct: 362  ASKELNNVKLEAESRRHDMEARQKDVEAAVAEVDSVTAKTTSIKESGASHQKQLARKCEE 421

Query: 491  IVNEFHSYSQSI 456
            I+ EF+ Y  SI
Sbjct: 422  IMKEFYQYQNSI 433


>ref|XP_006446114.1| hypothetical protein CICLE_v10015244mg [Citrus clementina]
            gi|568832784|ref|XP_006470606.1| PREDICTED: probable
            kinetochore protein NUF2-like [Citrus sinensis]
            gi|557548725|gb|ESR59354.1| hypothetical protein
            CICLE_v10015244mg [Citrus clementina]
            gi|641842355|gb|KDO61261.1| hypothetical protein
            CISIN_1g013334mg [Citrus sinensis]
          Length = 445

 Score =  405 bits (1042), Expect = e-110
 Identities = 219/436 (50%), Positives = 293/436 (67%), Gaps = 1/436 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPL-QDDHRQA 1572
            S F +P L   +IV IL E++IA I E  L NP  D V  +Y+ LLI+LD L +DD  Q 
Sbjct: 2    SKFDYPRLSATEIVTILTETEIAVITENDLKNPNPDFVSDLYTRLLIFLDVLHEDDQGQL 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            DF AL QLENPDLH  SV+++ L   + EV+ +V+CP +F L+DL +PD  R   FLSAI
Sbjct: 62   DFAALEQLENPDLHVRSVQIMKLYSMVKEVVTTVNCPMNFTLKDLIRPDATRTEYFLSAI 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNFCL+++TK+ LL+P  + +   +EQR+ELE +IS+L+ E++  NE  ERE P + EVD
Sbjct: 122  LNFCLYKDTKMNLLRPIAEDLTRLDEQRSELEDKISQLNAEIAAYNEVRERELPLVQEVD 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
             KVKELRQTIQ+LN HQ+ ++T+L  +K +   +DEK S  EF L+QS Q+NA L S+IV
Sbjct: 182  AKVKELRQTIQDLNKHQVSLRTTLNKLKEKTGALDEKFSQTEFDLVQSVQQNADLRSKIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSP+KLQ TLEEKKSIRE+ +++E+ AMQSFQEK+ TL+VY K LKKMSKQ AQM    E
Sbjct: 242  QSPEKLQRTLEEKKSIREEARDAEKLAMQSFQEKTTTLEVYQKTLKKMSKQSAQMQAILE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK++EKD K LKA+ ND+ +L  SLEAK +ERQGKV             +N+K E+
Sbjct: 302  QVNSAKSIEKDYKSLKARLNDDVVLDKSLEAKLIERQGKVEQLDELQQQLEKERNLKSED 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             T+  +         ++D  AR+ KVEA+V E D I  +     ESGAA   +L  K EE
Sbjct: 362  ATRAFENVKLEMESKRQDLEARQKKVEAVVTEVDAITSKTKFVKESGAAKLQELASKAEE 421

Query: 491  IVNEFHSYSQSIGSYL 444
            IV +F  Y+ SIG+ L
Sbjct: 422  IVEKFQQYTNSIGTLL 437


>ref|XP_012071329.1| PREDICTED: probable kinetochore protein NUF2 isoform X1 [Jatropha
            curcas] gi|643731625|gb|KDP38869.1| hypothetical protein
            JCGZ_05026 [Jatropha curcas]
          Length = 444

 Score =  402 bits (1034), Expect = e-109
 Identities = 220/433 (50%), Positives = 288/433 (66%), Gaps = 1/433 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQD-DHRQA 1572
            S F FP L R +IV IL ESQIA+I E  L NP +D +  +Y+ LLI+LD LQ+ D  Q 
Sbjct: 2    SKFEFPRLSRSEIVTILAESQIANITENDLKNPNADFLSDLYTRLLIHLDLLQEEDQGQV 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            +F AL QLENPD H DSVR++NLC +I E+++ V C   F L+DL +P   R   +LSAI
Sbjct: 62   EFAALEQLENPDHHVDSVRIMNLCIRIREMVSMVGCKLQFTLKDLLRPQGDRTEFYLSAI 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNFCLHR+ K+ LL+P VD + + +EQR E E +IS+L+ E+++ N A ERE P + EVD
Sbjct: 122  LNFCLHRDAKMNLLRPMVDDLTLLDEQRKEWEDKISQLNAEIADYNVARERELPLVQEVD 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
             KVKEL Q IQ LN+ QM ++ S+  +K +  E+D +IS AEF L+QS QENA L S+IV
Sbjct: 182  SKVKELYQIIQGLNSQQMSLRASIRKLKDKTGEMDTQISKAEFDLVQSVQENANLRSKIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSPDKLQ  LEEKK + E+ KN ERSAMQSFQEK+  L+VYSKALKKMSK L QM   +E
Sbjct: 242  QSPDKLQRALEEKKLVLEEAKNVERSAMQSFQEKTAILEVYSKALKKMSKHLNQMKTIQE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK++EKD K LKAK +D+ +L  SL+AK VE Q K              +++K EE
Sbjct: 302  QVNSAKSIEKDYKALKAKLSDDRMLDKSLDAKLVELQAKAQRSDELRKMLEKERDLKCEE 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             TK  +         + D  AR+ KVEA ++E D I  + NL  ESGAA   +L  KCEE
Sbjct: 362  ATKEYNNVKLEVESRRLDLEARQKKVEAALSEVDAITSKTNLVKESGAAKVQELVHKCEE 421

Query: 491  IVNEFHSYSQSIG 453
            I+ +F  Y +S+G
Sbjct: 422  IMEQFQEYKKSVG 434


>ref|XP_007029773.1| Kinetochore protein nuf2 isoform 1 [Theobroma cacao]
            gi|590639807|ref|XP_007029774.1| Kinetochore protein nuf2
            isoform 1 [Theobroma cacao] gi|508718378|gb|EOY10275.1|
            Kinetochore protein nuf2 isoform 1 [Theobroma cacao]
            gi|508718379|gb|EOY10276.1| Kinetochore protein nuf2
            isoform 1 [Theobroma cacao]
          Length = 445

 Score =  402 bits (1033), Expect = e-109
 Identities = 217/436 (49%), Positives = 298/436 (68%), Gaps = 1/436 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPL-QDDHRQA 1572
            S F +P+L R DI++IL ESQIA++ +    N   D V  +Y+ LLIYLD L ++D  Q 
Sbjct: 2    SKFEYPILSRADIISILAESQIAAVTDNDFKNVKPDFVSDLYTRLLIYLDALHEEDQGQV 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            +F+AL Q ENPDL   S++++NL  ++ EV+AS++CP  F LRDL KPD+ R   F+S+I
Sbjct: 62   EFSALEQFENPDLLIGSIQVMNLYCRLREVVASLNCPMQFNLRDLVKPDSARAEFFISSI 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNFCL+++TK+ LL+P  +++ + +EQR E E++IS+L+ E++  +EA ERE P + EVD
Sbjct: 122  LNFCLYKDTKMNLLRPIAEELTLLDEQRKEWEAKISQLNAEIAGYSEARERELPLVQEVD 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
             KVKELR+ I  LN++QM ++TS   +K +  ++DEKIS AEF L+QS QENA L S+IV
Sbjct: 182  SKVKELREMIAGLNSNQMSLRTSFRNLKDKTGQMDEKISKAEFDLVQSVQENANLRSKIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSPDKLQ  LEE+K  R++ K +ER AMQSFQEK+  ++VYSKALKKMSK  A M    E
Sbjct: 242  QSPDKLQRALEERKLARDEAKTAERLAMQSFQEKTTIVEVYSKALKKMSKHFALMQAIHE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK+VEK+ K LKAK +D+ +L  SLEAK +ERQGKV             +++K EE
Sbjct: 302  QVNSAKSVEKEWKGLKAKLSDDAVLDKSLEAKLIERQGKVEQLDELQRQLQKERDLKFEE 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             TK+L+         +RD  AR+ KVE +VAE D I  + ++  ESGAA  H L  KCEE
Sbjct: 362  STKHLNDVKLEVESRRRDLEARQRKVEDVVAEVDSITLKTSMAKESGAAKVHDLVSKCEE 421

Query: 491  IVNEFHSYSQSIGSYL 444
            IV +F  YS SIG  L
Sbjct: 422  IVKQFQHYSSSIGVLL 437


>ref|XP_012449504.1| PREDICTED: probable kinetochore protein NUF2 [Gossypium raimondii]
            gi|823233731|ref|XP_012449506.1| PREDICTED: probable
            kinetochore protein NUF2 [Gossypium raimondii]
            gi|763801109|gb|KJB68064.1| hypothetical protein
            B456_010G224800 [Gossypium raimondii]
            gi|763801111|gb|KJB68066.1| hypothetical protein
            B456_010G224800 [Gossypium raimondii]
          Length = 449

 Score =  401 bits (1031), Expect = e-109
 Identities = 219/436 (50%), Positives = 299/436 (68%), Gaps = 1/436 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPL-QDDHRQA 1572
            S+F +P+L R DI++IL ESQIA++ +    N   D V ++Y+ LLIYLD L ++D  Q 
Sbjct: 2    SSFDYPILSRSDIISILAESQIAAVTDNDFKNIKPDFVSNLYTRLLIYLDALNEEDQGQV 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            +F+AL Q+ENPDL   S +++NL  ++ EVMAS++CP  F LRDL KPD  R   FLS I
Sbjct: 62   EFSALEQIENPDLLIGSFQVMNLYCRLREVMASLNCPMQFNLRDLIKPDPRRTEHFLSGI 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNFCL++ETK+ LL+P V+++ + ++QR E E++IS+L+ E++  +EA ERE P + E++
Sbjct: 122  LNFCLYKETKMNLLRPIVEELALLDDQRKEWEAKISQLNEEIAGYSEARERELPLVQEIE 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
             KVKELR+ I  LN++QM ++TS   +K +  ++DEKIS AEF L+QS QENA L S+IV
Sbjct: 182  SKVKELREMIAGLNSNQMSLRTSFRNLKEKTGQMDEKISKAEFDLVQSVQENANLRSKIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSPDKLQ  LEEKK  R++ K +ERSAMQSFQEK+ T++VYSKALKKMSK  A M    E
Sbjct: 242  QSPDKLQRALEEKKLARDEAKYAERSAMQSFQEKTATVEVYSKALKKMSKHFALMQAIHE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK+VEK+ K LKAK +D+ +L  SLEAK +ERQGKV              ++K EE
Sbjct: 302  QVNSAKSVEKECKGLKAKLSDDVVLDKSLEAKLIERQGKVVQLEEHKRQLQKESDLKFEE 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             TK+L+         + +  AR+ KVE +VAE D I  + ++  ESGAA   QL  KCEE
Sbjct: 362  STKHLNSVKSEVLSKRCELEARQKKVEDVVAEVDSITLKTSMVRESGAAKVQQLISKCEE 421

Query: 491  IVNEFHSYSQSIGSYL 444
            IV +F  YS SIG  L
Sbjct: 422  IVKQFQQYSSSIGLLL 437


>ref|XP_012445609.1| PREDICTED: probable kinetochore protein NUF2 isoform X1 [Gossypium
            raimondii]
          Length = 449

 Score =  399 bits (1024), Expect = e-108
 Identities = 217/436 (49%), Positives = 300/436 (68%), Gaps = 1/436 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPL-QDDHRQA 1572
            S+F +P+L R DI++IL ESQIA++ +    N   D + ++Y+ LLIYLD L ++D  Q 
Sbjct: 2    SSFDYPILSRSDIISILAESQIAAVTDNDFKNIKPDFISNLYTRLLIYLDALNEEDQGQV 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            +F+AL Q+ENPDL   S +++NL  ++ EVMAS++CP  F LRDL KPD  R   FLS I
Sbjct: 62   EFSALEQIENPDLLIGSFQVMNLYCRLREVMASLNCPMQFNLRDLIKPDPRRTEHFLSGI 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNFCL++ETK+ LL+P V+++ + ++QR E E++IS+L+ E++  +EA ERE P + EV+
Sbjct: 122  LNFCLYKETKMNLLRPIVEELALLDDQRKEWEAKISQLNAEIAGYSEARERELPLVQEVE 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
             KVKELR+ I  LN++QM ++TS   +K +  ++DEKIS AEF L+QS QENA L S+IV
Sbjct: 182  SKVKELREMIAGLNSNQMSLRTSFRNLKEKTGQMDEKISKAEFDLVQSVQENANLRSKIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSPDKLQ  LEEKK  R++ KN+ERSA+QSFQEK+ T++VYSKALKKMSK  A M    E
Sbjct: 242  QSPDKLQRALEEKKLARDEAKNAERSAIQSFQEKTATVEVYSKALKKMSKHFALMQAIHE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK+VEK+ K LKAK +D+ +L  SLEAK +E +GKV             +++K EE
Sbjct: 302  QVNSAKSVEKECKGLKAKLSDDVVLDKSLEAKLIEWEGKVGQLDEHKRQLQKERDLKFEE 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             TK+L+         + +  AR+ KVE +VAE D I  + ++  ESGAA   QL  KCEE
Sbjct: 362  STKHLNSLKSEVLSKRCELEARQKKVEDVVAEVDSITIKTSMVRESGAAKVQQLISKCEE 421

Query: 491  IVNEFHSYSQSIGSYL 444
            IV +F  YS SIG  L
Sbjct: 422  IVKQFQQYSSSIGLLL 437


>ref|XP_011023065.1| PREDICTED: kinetochore protein nuf2 [Populus euphratica]
          Length = 442

 Score =  397 bits (1020), Expect = e-107
 Identities = 214/432 (49%), Positives = 288/432 (66%), Gaps = 1/432 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPL-QDDHRQA 1572
            S F +P+L R D+++IL ESQIA+I E  L NPT D V+ IY+ LL+YLD L ++D  Q 
Sbjct: 2    SKFEYPILSRTDMISILAESQIAAIIETDLKNPTPDFVVDIYTRLLVYLDLLHEEDEGQV 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            +F AL QLENP  H  S R++NL  K+ EV+  + CP +F LRDL KP   R   FLSAI
Sbjct: 62   EFAALEQLENPHYHVGSARIMNLYIKVKEVITMLQCPAEFTLRDLLKPQGDRTQFFLSAI 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNFCLH+++K+  L+P  +++ + +EQR  LE +IS+L+ E++  N+A ERE P + EVD
Sbjct: 122  LNFCLHKDSKMNELRPIGEELTLLDEQRRGLEDKISQLNAEIAEYNDARERELPLVQEVD 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
            GKVKELR+ I +LNNHQM ++ S   +K R++E+D +IS AEF L+QS QENA L S+IV
Sbjct: 182  GKVKELRKKIADLNNHQMSLRASYRKLKERSSEMDGEISRAEFDLVQSVQENANLRSKIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSPDKLQ  LEEKKS+RE+ +N+ER AMQSF+ KS  L+VY+KALKKMSK   QM    E
Sbjct: 242  QSPDKLQRALEEKKSVREEARNAERLAMQSFEAKSAVLEVYTKALKKMSKHFDQMQAIHE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK++EKD K LKAK +D+GL   SL+AK VE Q K              ++MK  E
Sbjct: 302  QVNSAKSIEKDYKALKAKLSDDGLTDKSLDAKLVELQMKAQQLNELKKLLEKERDMKCAE 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             +K  +         +RD  AR+ +VEA+++E D I  + N+  ESGAA   +L  K EE
Sbjct: 362  ASKEYNTIKSEVESKRRDLEARQRRVEAVLSEVDAITSKTNMVNESGAAKVQKLVSKREE 421

Query: 491  IVNEFHSYSQSI 456
            I  +F  Y  SI
Sbjct: 422  IAEQFQKYKNSI 433


>ref|XP_009355229.1| PREDICTED: probable kinetochore protein NUF2 [Pyrus x bretschneideri]
          Length = 441

 Score =  397 bits (1020), Expect = e-107
 Identities = 215/440 (48%), Positives = 292/440 (66%), Gaps = 1/440 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPL-QDDHRQA 1572
            S F +P L R DIV IL ++ I +I +  LVNP  D V  +Y+ +L+ LD   ++D  Q 
Sbjct: 2    SKFEYPKLTRSDIVTILADAHIVAISDRDLVNPNPDFVADLYTRILVSLDFFHEEDFGQV 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            +F+AL QLENPD H DS R + L  +I EV+A V CP  F L+DL KP+T R   F+SA+
Sbjct: 62   EFSALEQLENPDFHMDSARTMKLYNRIKEVVALVDCPKRFTLKDLIKPETDRTEYFVSAL 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNF LHRETK+ +L   VDQ+   +E+R   E +IS+L+ E+++ NEA E+E P + EVD
Sbjct: 122  LNFSLHRETKMNILTQVVDQLTDIDERRKGWEDKISQLNAEIADYNEAREKELPLVQEVD 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
             KVKEL QT+  LNN Q  ++TS   +K +  EI+EK+SSAEF+L+QS QENA L SRIV
Sbjct: 182  AKVKELHQTVSGLNNQQKSLRTSRQKLKEKIGEIEEKVSSAEFSLVQSVQENANLRSRIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSPDKLQ  LEEKKS+RE+ KN+ERSA QSF+EK+   +VY+K  KK+SK LAQM   +E
Sbjct: 242  QSPDKLQRALEEKKSVREEAKNAERSAKQSFEEKTAVDEVYTKVSKKLSKHLAQMQAIQE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK+V++DVK +KAK +D+G++  SL+AK VER+GKV             +++K EE
Sbjct: 302  QVNSAKSVDRDVKAVKAKLSDDGVVSKSLQAKLVEREGKVEQLNELKKQLERERDVKFEE 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             +K+L+         +RD  AR+  VEA V E D I  +     E+GAA + +L +KCEE
Sbjct: 362  ASKDLNNVELEVESRRRDLEARQKAVEAAVEEVDSITSKTASIKEAGAADQKELARKCEE 421

Query: 491  IVNEFHSYSQSIGSYLDGVQ 432
            I+ EFH Y  SI   L G Q
Sbjct: 422  IMKEFHQYQNSIRVLLLGSQ 441


>ref|XP_011087745.1| PREDICTED: probable kinetochore protein NUF2 isoform X1 [Sesamum
            indicum]
          Length = 442

 Score =  397 bits (1019), Expect = e-107
 Identities = 209/433 (48%), Positives = 292/433 (67%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQDDHRQAD 1569
            S F +P LPR +I+A L +SQIA++ E  L+ P  D++ ++Y+ + + +D LQ+D  Q +
Sbjct: 2    SKFEYPRLPRHEILAALADSQIATLSEADLLRPDPDLIFNLYTQIFLLIDTLQEDQGQME 61

Query: 1568 FNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAIL 1389
            F AL  LENPD H  SV+++NL  KI E++A+V+CP  F  +DL KP+T R  LFLSA+L
Sbjct: 62   FGALELLENPDHHMHSVQVMNLYNKIRELVAAVNCPKAFTPKDLIKPETERTELFLSALL 121

Query: 1388 NFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVDG 1209
            NF ++RETKL LL+P  D +N+ EE+    + R+S+L+ E++   E  ERE P + EV+ 
Sbjct: 122  NFYIYRETKLDLLKPIGDDLNLFEERELAAKDRMSQLNAEIAECEELREREVPLVQEVNS 181

Query: 1208 KVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIVQ 1029
            KVKEL QT+  LN HQM +KTS+  +K +A E+DEKIS AEF L+QS QENA L S+IVQ
Sbjct: 182  KVKELHQTVSGLNKHQMTLKTSIKQLKEKAKEMDEKISEAEFALVQSVQENANLRSKIVQ 241

Query: 1028 SPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKEQ 849
            SPDKLQ  LEEKK ++ + KN+ER+A+Q+FQ+K+  L+ Y+KA KKMSK  AQM   +EQ
Sbjct: 242  SPDKLQRALEEKKLVQIETKNAERAAVQAFQDKTTILEAYAKACKKMSKHFAQMQAVQEQ 301

Query: 848  ADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEEV 669
             ++AK+V+KDVKVLK+K +DEG+L  SLEAK VE Q                  +KHEEV
Sbjct: 302  VNSAKSVDKDVKVLKSKLSDEGVLIKSLEAKVVELQSIADQLKEFRRQLEKETALKHEEV 361

Query: 668  TKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEEI 489
            TK L+         ++    R  +VE++V+E D II + N+  E G AT  +L  KCEEI
Sbjct: 362  TKELNNVKLEVGSKRKALEVRHRQVESVVSEADAIISKKNIVKEEGEATMQELGSKCEEI 421

Query: 488  VNEFHSYSQSIGS 450
            V EF++YS+ I +
Sbjct: 422  VAEFNNYSKLISN 434


>ref|XP_012071330.1| PREDICTED: probable kinetochore protein NUF2 isoform X2 [Jatropha
            curcas]
          Length = 435

 Score =  396 bits (1018), Expect = e-107
 Identities = 220/434 (50%), Positives = 286/434 (65%), Gaps = 1/434 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQD-DHRQA 1572
            S F FP L R +IV IL ESQIA+I E  L NP +D +  +Y+ LLI+LD LQ+ D  Q 
Sbjct: 2    SKFEFPRLSRSEIVTILAESQIANITENDLKNPNADFLSDLYTRLLIHLDLLQEEDQGQV 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            +F AL QLENPD H DSVR++NLC +I E+++ V C   F L+DL +P   R   +LSAI
Sbjct: 62   EFAALEQLENPDHHVDSVRIMNLCIRIREMVSMVGCKLQFTLKDLLRPQGDRTEFYLSAI 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNFCLHR+ K+ LL+P VD + + +EQR E E +IS+L+ E+++ N A ERE P + EVD
Sbjct: 122  LNFCLHRDAKMNLLRPMVDDLTLLDEQRKEWEDKISQLNAEIADYNVARERELPLVQEVD 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
             KVKEL Q IQ LN+ QM ++ S+  +K +  E+D +IS AEF L+QS QENA L S+IV
Sbjct: 182  SKVKELYQIIQGLNSQQMSLRASIRKLKDKTGEMDTQISKAEFDLVQSVQENANLRSKIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSPDKLQ  LEEKK + E+ KN ERSAMQSFQEK+  L+VYSKALKKMSK L QM   +E
Sbjct: 242  QSPDKLQRALEEKKLVLEEAKNVERSAMQSFQEKTAILEVYSKALKKMSKHLNQMKTIQE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK++EKD K LKAK +D+ +L  SL+AK VE Q K              +++K EE
Sbjct: 302  QVNSAKSIEKDYKALKAKLSDDRMLDKSLDAKLVELQAKAQRSDELRKMLEKERDLKCEE 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             TK  +         + D  AR+ KVEA ++E D I  + NL  ESGAA   +L  KCEE
Sbjct: 362  ATKEYNNVKLEVESRRLDLEARQKKVEAALSEVDAITSKTNLVKESGAAKVQELVHKCEE 421

Query: 491  IVNEFHSYSQSIGS 450
            I+ + H    SI S
Sbjct: 422  IMEQVHIIPLSITS 435


>ref|XP_009804688.1| PREDICTED: kinetochore protein NDC80 homolog isoform X1 [Nicotiana
            sylvestris]
          Length = 450

 Score =  395 bits (1016), Expect = e-107
 Identities = 202/431 (46%), Positives = 289/431 (67%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQDDHRQAD 1569
            S F +P L R+DI+A+L ESQ+A++ ++ L+ PT D V ++YS +L+++D LQ+D  Q D
Sbjct: 2    SRFDYPTLSRQDIIAVLAESQLATVSDQDLLKPTPDFVTNLYSTVLLHVDSLQEDDDQVD 61

Query: 1568 FNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAIL 1389
            F+AL  LENPD+H +S R++NL  KI E++ ++ CP  F LRDL KPD  R   F+ A+L
Sbjct: 62   FSALEHLENPDVHVESFRIMNLFHKIREMLTTLDCPEPFTLRDLIKPDPERTRFFVGAML 121

Query: 1388 NFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVDG 1209
            NF LHR+TKL  + P V+++ + +EQR  LE+RIS+++ E++   E+ ERE P + ++D 
Sbjct: 122  NFSLHRDTKLSAISPIVEELTLIDEQRRALEARISQINEEIAEYTESREREMPMVQQIDS 181

Query: 1208 KVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIVQ 1029
             VKELRQTI  LNNHQM +KTSL  +K R  E+DEKIS+A+F L+Q+  ENA L S+IVQ
Sbjct: 182  NVKELRQTISGLNNHQMSLKTSLKKLKERTKEMDEKISNADFALVQAVHENANLRSKIVQ 241

Query: 1028 SPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKEQ 849
            SP KLQ  LEEKKS + + +N+ER+AMQSFQ K+  L+VY+KA +KMSK   QM   +EQ
Sbjct: 242  SPVKLQRALEEKKSFQAEARNAERAAMQSFQNKTSILEVYTKAHQKMSKHFNQMQAIQEQ 301

Query: 848  ADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEEV 669
             ++ K++EKD+K LK K +DE +L  SLEAK VERQGK              +++  EE 
Sbjct: 302  VNSTKSIEKDIKALKQKLSDEEVLDKSLEAKLVERQGKADQLEELRKQLKKERDLSCEEA 361

Query: 668  TKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEEI 489
             K ++         +    AR+ +VE ++AE D I  + N   ESGA+   +L + CEE+
Sbjct: 362  AKEMNFFNLEMESKRHGLEARQKEVEGVLAEADAITAKINSVRESGASKCQELGRHCEEV 421

Query: 488  VNEFHSYSQSI 456
            V EF+ +S SI
Sbjct: 422  VAEFYRHSSSI 432


>ref|XP_002309861.2| hypothetical protein POPTR_0007s03160g [Populus trichocarpa]
            gi|550334021|gb|EEE90311.2| hypothetical protein
            POPTR_0007s03160g [Populus trichocarpa]
          Length = 442

 Score =  395 bits (1014), Expect = e-107
 Identities = 215/432 (49%), Positives = 286/432 (66%), Gaps = 1/432 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPL-QDDHRQA 1572
            S F +P+L R D+++IL ESQIA+I E  L NPT D V  IY+ LL+YLD L ++D  Q 
Sbjct: 2    SKFEYPMLSRTDMISILAESQIAAIIETDLKNPTPDFVADIYTRLLVYLDLLHEEDEGQV 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            +F AL QLENP  H  S R++NL  K+ EV+  +  P DF LRDL KP   R   FLSAI
Sbjct: 62   EFAALEQLENPHYHVGSARIMNLYIKVKEVITMLQSPADFTLRDLLKPQGDRTQFFLSAI 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNFCLH+++K+  L+P  +++ + +EQR  LE +IS+L+ E++  N+A ERE P   EVD
Sbjct: 122  LNFCLHKDSKMNELRPIGEELTLLDEQRRGLEDKISQLNAEIAEYNDARERELPLAQEVD 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
            GKVKELRQ I +LNNHQM ++ S   +K R++E+D +IS AEF L+QS QENA L S+IV
Sbjct: 182  GKVKELRQEIADLNNHQMSLRASYRKLKERSSEMDGEISRAEFDLVQSVQENANLRSKIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSPDKLQ  LEEKKS+RE+ +N+ER AMQSF+ K+  L+VY+KALKKMSK   QM    E
Sbjct: 242  QSPDKLQRALEEKKSVREEARNAERLAMQSFEAKTAVLEVYTKALKKMSKHFDQMQAIHE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK++EKD K LKAK +D+GL+  SL+AK VE Q K              ++MK EE
Sbjct: 302  QVNSAKSIEKDYKALKAKLSDDGLMDKSLDAKLVELQMKAQQLNELKKLLEKERDMKCEE 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             TK  +         + D  AR+ +VEA+++E D I  + N+  ESGAA   +L  K EE
Sbjct: 362  ATKEYNTIKSEVESKRHDLEARQRRVEAVLSEVDAITSKTNMVNESGAAKVQKLVSKREE 421

Query: 491  IVNEFHSYSQSI 456
            I  +F  Y  SI
Sbjct: 422  IAEQFKKYKNSI 433


>ref|XP_009622956.1| PREDICTED: huntingtin-interacting protein 1-related protein isoform
            X1 [Nicotiana tomentosiformis]
          Length = 450

 Score =  393 bits (1010), Expect = e-106
 Identities = 201/430 (46%), Positives = 287/430 (66%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPLQDDHRQAD 1569
            S F +P L R+DI+A+L ESQ+A++ ++ L+ PT D V ++YS +L+++D LQ+D  Q D
Sbjct: 2    SRFDYPTLSRQDIIAVLAESQLATVSDQDLLKPTPDFVTNLYSTVLLHVDSLQEDDDQVD 61

Query: 1568 FNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAIL 1389
            F AL  LENPD+H DS R++NL  KI E++ ++ CP  F LRDL KPD  R   F+ A+L
Sbjct: 62   FAALEHLENPDVHVDSFRIMNLFHKIREMLTTLDCPEPFTLRDLIKPDPERTRFFVGAML 121

Query: 1388 NFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVDG 1209
            NF LHR+TKL  + P V+++ + +EQR  +E+RIS+++ E++   E+ ERE P + E+D 
Sbjct: 122  NFSLHRDTKLSAISPIVEELTLIDEQRRAVEARISQINEEIAEYTESREREMPLVQEIDS 181

Query: 1208 KVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIVQ 1029
             VKELRQTI  LNNHQM +K SL  +K R+ E+DEKIS+A+F L+Q+  ENA L S+IVQ
Sbjct: 182  NVKELRQTISGLNNHQMSLKASLKKLKERSKEMDEKISNADFVLVQAVHENANLRSKIVQ 241

Query: 1028 SPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKEQ 849
            SP KLQ  LEEKKS + + +N+ER+AMQSFQ K+  L+VY+KA +KMSK   QM   +EQ
Sbjct: 242  SPVKLQRALEEKKSFQAEARNAERAAMQSFQNKTSILEVYTKAHQKMSKHFNQMQAIQEQ 301

Query: 848  ADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEEV 669
             ++ K++EKD+KVLK K +DE +L  SLEAK VERQGK              +++  EE 
Sbjct: 302  VNSTKSIEKDIKVLKQKLSDEEVLDKSLEAKLVERQGKADQLEELRKQLKKERDLSCEEA 361

Query: 668  TKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEEI 489
             K +          +    AR+ +VE ++AE D +  + N   ESGA+   +L + CEE+
Sbjct: 362  AKEMKFFNLEMESKRHGLEARQKEVEGVLAEADAVTAKINSVRESGASKCQELGRHCEEV 421

Query: 488  VNEFHSYSQS 459
            V EF+ +S S
Sbjct: 422  VAEFYRHSSS 431


>gb|KJB68067.1| hypothetical protein B456_010G224800 [Gossypium raimondii]
          Length = 431

 Score =  389 bits (999), Expect = e-105
 Identities = 212/424 (50%), Positives = 292/424 (68%), Gaps = 1/424 (0%)
 Frame = -1

Query: 1748 STFSFPVLPRKDIVAILHESQIASIKEEHLVNPTSDVVLSIYSNLLIYLDPL-QDDHRQA 1572
            S+F +P+L R DI++IL ESQIA++ +    N   D V ++Y+ LLIYLD L ++D  Q 
Sbjct: 2    SSFDYPILSRSDIISILAESQIAAVTDNDFKNIKPDFVSNLYTRLLIYLDALNEEDQGQV 61

Query: 1571 DFNALGQLENPDLHGDSVRLVNLCRKINEVMASVSCPTDFLLRDLAKPDTHRNGLFLSAI 1392
            +F+AL Q+ENPDL   S +++NL  ++ EVMAS++CP  F LRDL KPD  R   FLS I
Sbjct: 62   EFSALEQIENPDLLIGSFQVMNLYCRLREVMASLNCPMQFNLRDLIKPDPRRTEHFLSGI 121

Query: 1391 LNFCLHRETKLVLLQPFVDQVNIHEEQRNELESRISELHMEMSNLNEANEREQPFIAEVD 1212
            LNFCL++ETK+ LL+P V+++ + ++QR E E++IS+L+ E++  +EA ERE P + E++
Sbjct: 122  LNFCLYKETKMNLLRPIVEELALLDDQRKEWEAKISQLNEEIAGYSEARERELPLVQEIE 181

Query: 1211 GKVKELRQTIQELNNHQMLVKTSLLAVKGRATEIDEKISSAEFTLIQSAQENAKLLSRIV 1032
             KVKELR+ I  LN++QM ++TS   +K +  ++DEKIS AEF L+QS QENA L S+IV
Sbjct: 182  SKVKELREMIAGLNSNQMSLRTSFRNLKEKTGQMDEKISKAEFDLVQSVQENANLRSKIV 241

Query: 1031 QSPDKLQGTLEEKKSIREDVKNSERSAMQSFQEKSCTLDVYSKALKKMSKQLAQMHGTKE 852
            QSPDKLQ  LEEKK  R++ K +ERSAMQSFQEK+ T++VYSKALKKMSK  A M    E
Sbjct: 242  QSPDKLQRALEEKKLARDEAKYAERSAMQSFQEKTATVEVYSKALKKMSKHFALMQAIHE 301

Query: 851  QADAAKTVEKDVKVLKAKQNDEGLLGMSLEAKSVERQGKVXXXXXXXXXXXXXKNMKHEE 672
            Q ++AK+VEK+ K LKAK +D+ +L  SLEAK +ERQGKV              ++K EE
Sbjct: 302  QVNSAKSVEKECKGLKAKLSDDVVLDKSLEAKLIERQGKVVQLEEHKRQLQKESDLKFEE 361

Query: 671  VTKNLDXXXXXXXXXKRDFAARKVKVEALVAEGDGIIKRNNLEIESGAATKHQLYKKCEE 492
             TK+L+         + +  AR+ KVE +VAE D I  + ++  ESGAA   QL  KCEE
Sbjct: 362  STKHLNSVKSEVLSKRCELEARQKKVEDVVAEVDSITLKTSMVRESGAAKVQQLISKCEE 421

Query: 491  IVNE 480
            IV +
Sbjct: 422  IVKQ 425