BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver30_contig00031735
         (420 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; Alt...   235   9e-60
gb|ACO90230.1| cheilanthifoline synthase-like protein [Papaver b...   150   5e-34
gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum]         147   4e-33
sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase;...   139   6e-31
sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase;...   125   2e-26
dbj|BAG75114.1| cytochrome P450 [Eschscholzia californica]            120   4e-25
gb|AFB74615.1| cytochrome P450 [Papaver somniferum]                   119   7e-25
gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum]          119   7e-25
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   118   2e-24
gb|ADB89214.1| stylopine synthase [Papaver somniferum]                117   3e-24
gb|ACO90235.1| stylopine synthase-like protein [Papaver bracteatum]   117   3e-24
gb|AGL76711.1| (S)-canadine synthase, partial [Coptis chinensis]      117   4e-24
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   116   8e-24
gb|ACO90219.1| stylopine synthase [Eschscholzia californica]          115   1e-23
sp|Q50LH3.1|C7192_ESCCA RecName: Full=(S)-stylopine synthase 1; ...   115   1e-23
dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       114   3e-23
gb|AHF65153.1| stylopine synthase, partial [synthetic construct]      112   8e-23
dbj|BAG75116.1| cytochrome P450 [Eschscholzia californica]            111   2e-22
sp|Q50LH4.1|C7193_ESCCA RecName: Full=(S)-stylopine synthase 2; ...   110   3e-22
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       110   5e-22

>sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; AltName: Full=Cytochrome P450
           719B1 gi|148841090|gb|ABR14720.1| salutaridine synthase
           [Papaver somniferum] gi|827520611|gb|AKJ85663.1|
           salutaridine synthase [synthetic construct]
          Length = 505

 Score =  235 bits (600), Expect = 9e-60
 Identities = 115/115 (100%), Positives = 115/115 (100%)
 Frame = -2

Query: 347 MAPINIEGNDFWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLG 168
           MAPINIEGNDFWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLG
Sbjct: 1   MAPINIEGNDFWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLG 60

Query: 167 GGVPLQVALANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           GGVPLQVALANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV
Sbjct: 61  GGVPLQVALANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 115


>gb|ACO90230.1| cheilanthifoline synthase-like protein [Papaver bracteatum]
          Length = 271

 Score =  150 bits (378), Expect = 5e-34
 Identities = 74/108 (68%), Positives = 83/108 (76%)
 Frame = -2

Query: 326 GNDFWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQV 147
           G  FW+I C V++ FAL K +F K S   S +T EWPAGPKTLPIIGNLHQLGG     V
Sbjct: 2   GETFWLITCGVVVAFALAKLLFGKKS---SLSTMEWPAGPKTLPIIGNLHQLGGEA-FHV 57

Query: 146 ALANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
            LANLAKVYGG FTIW+GSW P IVISD+D A EVLVNKS+DYSARD+
Sbjct: 58  CLANLAKVYGGVFTIWVGSWRPFIVISDVDKAWEVLVNKSSDYSARDM 105


>gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum]
          Length = 492

 Score =  147 bits (370), Expect = 4e-33
 Identities = 72/105 (68%), Positives = 82/105 (78%)
 Frame = -2

Query: 317 FWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVALA 138
           FW+I C V++ FAL K +F K S   S +T EWP+GPKTLPIIGNLHQLGG     V LA
Sbjct: 5   FWLITCGVVVAFALAKLLFGKKS---SMSTMEWPSGPKTLPIIGNLHQLGGEA-FHVCLA 60

Query: 137 NLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           NLAKVYGG FTIW+GSW P IVISD+D A EVLVNKS+DYSARD+
Sbjct: 61  NLAKVYGGVFTIWVGSWRPFIVISDVDKAWEVLVNKSSDYSARDM 105


>sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
           Full=Cytochrome P450 719A14 [Argemone mexicana]
           gi|148841094|gb|ABR14722.1| cheilanthifoline synthase
           [Argemone mexicana]
          Length = 494

 Score =  139 bits (351), Expect = 6e-31
 Identities = 70/104 (67%), Positives = 79/104 (75%)
 Frame = -2

Query: 314 WMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVALAN 135
           W+I  TVIIV  + KF+  K S   S +T EWP GPK LPIIGNLHQLGG V   V LAN
Sbjct: 6   WLIISTVIIVLGIAKFLLGKSSS-SSLSTMEWPVGPKKLPIIGNLHQLGGDV-FHVVLAN 63

Query: 134 LAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           LAKVYG  FTIW+GSW PMI++SDID A EVLVNKS+DYSARD+
Sbjct: 64  LAKVYGSVFTIWVGSWRPMIIVSDIDKAWEVLVNKSSDYSARDM 107


>sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
           Full=Cytochrome P450 719A5 [Eschscholzia californica]
           gi|209571368|dbj|BAG75113.1| cheilanthifoline synthase
           [Eschscholzia californica]
          Length = 490

 Score =  125 bits (313), Expect = 2e-26
 Identities = 61/103 (59%), Positives = 74/103 (71%)
 Frame = -2

Query: 314 WMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVALAN 135
           W++  TV++VFA+ K +        S +T EWP GPK LPIIGNLHQLGG     V LAN
Sbjct: 6   WVVTATVVVVFAIAKLLKKS----SSISTMEWPKGPKKLPIIGNLHQLGGEA-FHVVLAN 60

Query: 134 LAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARD 6
           LAK++G   TIW+G+W PMIVISDID A EVLVNKS+DY+ RD
Sbjct: 61  LAKIHGTVMTIWVGAWRPMIVISDIDKAWEVLVNKSSDYAGRD 103


>dbj|BAG75114.1| cytochrome P450 [Eschscholzia californica]
          Length = 495

 Score =  120 bits (301), Expect = 4e-25
 Identities = 61/108 (56%), Positives = 77/108 (71%), Gaps = 1/108 (0%)
 Frame = -2

Query: 323 NDFWMIACT-VIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQV 147
           N+ W++  T   ++ ++  F+  K S       TEWP GPKTLPIIGN+H LGG   LQV
Sbjct: 10  NNPWVLTATFTTLLISIFLFVTRKTS------NTEWPNGPKTLPIIGNMHLLGGTKALQV 63

Query: 146 ALANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
            L NLAKVYGG  TIWIGSW P+I++SDI+ A EVLVNKS+DYSAR++
Sbjct: 64  ILHNLAKVYGGVMTIWIGSWRPVIIVSDIERAWEVLVNKSSDYSAREL 111


>gb|AFB74615.1| cytochrome P450 [Papaver somniferum]
          Length = 486

 Score =  119 bits (299), Expect = 7e-25
 Identities = 62/109 (56%), Positives = 78/109 (71%), Gaps = 2/109 (1%)
 Frame = -2

Query: 323 NDFWMIAC--TVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQ 150
           ++ W++    T++ VFA V  +F +     SA+TTEWP GPKTLPIIGNLH LGG   L 
Sbjct: 4   SNLWILTLISTILAVFAAVLIIFRRRI---SASTTEWPVGPKTLPIIGNLHILGG-TALH 59

Query: 149 VALANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           V L  LA+VYG   TIWIGSW P+I++SD D A EVLVNKS+DYSAR++
Sbjct: 60  VVLHKLAEVYGSVMTIWIGSWKPVIIVSDFDRAWEVLVNKSSDYSAREM 108


>gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum]
          Length = 488

 Score =  119 bits (299), Expect = 7e-25
 Identities = 62/109 (56%), Positives = 78/109 (71%), Gaps = 2/109 (1%)
 Frame = -2

Query: 323 NDFWMIAC--TVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQ 150
           ++ W++    T++ VFA V  +F +     SA+TTEWP GPKTLPIIGNLH LGG   L 
Sbjct: 4   SNLWILTLISTILAVFAAVLIIFRRRI---SASTTEWPVGPKTLPIIGNLHILGG-TALH 59

Query: 149 VALANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           V L  LA+VYG   TIWIGSW P+I++SD D A EVLVNKS+DYSAR++
Sbjct: 60  VVLHKLAEVYGSVMTIWIGSWKPVIIVSDFDRAWEVLVNKSSDYSAREM 108


>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
           Full=(S)-tetrahydrocolumbamine oxidase
           (methylenedioxy-bridge-forming); AltName:
           Full=Cytochrome P450 719A1 [Coptis japonica]
           gi|15822820|dbj|BAB68769.1| methylenedioxy
           bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  118 bits (295), Expect = 2e-24
 Identities = 64/106 (60%), Positives = 77/106 (72%), Gaps = 3/106 (2%)
 Frame = -2

Query: 311 MIACTVIIVFA---LVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVAL 141
           ++  TV IVFA   +++ +FS  S  Q     +WP+GP+ LPIIGNLHQLG  V L VAL
Sbjct: 7   LVCATVAIVFATTTIIRILFSSSSLPQM----KWPSGPRKLPIIGNLHQLGDDV-LHVAL 61

Query: 140 ANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           A LAKV+G   TIWIGSW P+IVISDI+ A EVLVNKSADY ARD+
Sbjct: 62  AKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVLVNKSADYGARDM 107


>gb|ADB89214.1| stylopine synthase [Papaver somniferum]
          Length = 494

 Score =  117 bits (293), Expect = 3e-24
 Identities = 56/104 (53%), Positives = 75/104 (72%)
 Frame = -2

Query: 314 WMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVALAN 135
           W++  + ++V  ++  +   +   + ++T  WP GPKTLPIIGN+H LGG   LQV L N
Sbjct: 10  WILTTSTLVVITIITMLM--VFKRKKSSTMVWPTGPKTLPIIGNMHVLGG-TALQVVLHN 66

Query: 134 LAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           LAK++G   TIWIGSW P+IV+SDID A EVLVNKS+DYSARD+
Sbjct: 67  LAKIHGNVMTIWIGSWRPVIVVSDIDRAWEVLVNKSSDYSARDM 110


>gb|ACO90235.1| stylopine synthase-like protein [Papaver bracteatum]
          Length = 274

 Score =  117 bits (293), Expect = 3e-24
 Identities = 56/104 (53%), Positives = 75/104 (72%)
 Frame = -2

Query: 314 WMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVALAN 135
           W++  + ++V  ++  +   +   + ++T  WP GPKTLPIIGN+H LGG   LQV L N
Sbjct: 10  WILTTSTLVVITIITMLM--VFKRKKSSTMVWPTGPKTLPIIGNMHVLGG-TALQVVLHN 66

Query: 134 LAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           LAK++G   TIWIGSW P+IV+SDID A EVLVNKS+DYSARD+
Sbjct: 67  LAKIHGNVMTIWIGSWRPVIVVSDIDRAWEVLVNKSSDYSARDM 110


>gb|AGL76711.1| (S)-canadine synthase, partial [Coptis chinensis]
          Length = 491

 Score =  117 bits (292), Expect = 4e-24
 Identities = 63/106 (59%), Positives = 77/106 (72%), Gaps = 3/106 (2%)
 Frame = -2

Query: 311 MIACTVIIVFA---LVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVAL 141
           ++  TV IVFA   +++ +FS  S  Q     +WP+GP+ LPIIGNLHQLG  V L VAL
Sbjct: 7   LVCATVAIVFATTTIIRILFSSSSLPQM----KWPSGPRKLPIIGNLHQLGDDV-LHVAL 61

Query: 140 ANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           A LAKV+G   TIWIGSW P+IVISD++ A EVLVNKSADY ARD+
Sbjct: 62  AKLAKVHGSMMTIWIGSWRPVIVISDLEKAWEVLVNKSADYGARDM 107


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  116 bits (290), Expect = 8e-24
 Identities = 57/104 (54%), Positives = 75/104 (72%)
 Frame = -2

Query: 314 WMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVALAN 135
           W+++ T+ +V A+   +   ++   S +  +WP GP  LPIIGNLHQLGG V L VALA 
Sbjct: 7   WLVSATLALVVAIT-ILIPMVTSSSSFSQMKWPTGPNKLPIIGNLHQLGGDV-LHVALAK 64

Query: 134 LAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           LA+V+G   T+WIG+W P+IVISDID A EVLVNKS+DY ARD+
Sbjct: 65  LAQVHGSVMTVWIGNWRPIIVISDIDKAWEVLVNKSSDYGARDM 108


>gb|ACO90219.1| stylopine synthase [Eschscholzia californica]
          Length = 495

 Score =  115 bits (288), Expect = 1e-23
 Identities = 60/107 (56%), Positives = 76/107 (71%)
 Frame = -2

Query: 323 NDFWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVA 144
           N+ W++  T   +   + F+F    F + ++   WPAGPKTLPIIGN+H LGG   LQV 
Sbjct: 10  NNPWILTATATTLLISI-FLF----FTRKSSKMVWPAGPKTLPIIGNMHLLGG-TALQVV 63

Query: 143 LANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           L NLAKV+G   TIWIGSW P+IV+SDI+ A EVLVNKS+DYSARD+
Sbjct: 64  LHNLAKVHGSVMTIWIGSWRPVIVVSDIERAWEVLVNKSSDYSARDM 110


>sp|Q50LH3.1|C7192_ESCCA RecName: Full=(S)-stylopine synthase 1; Short=STS; AltName:
           Full=Cytochrome P450 719A2; Short=EcCYP719A2;
           Short=EcCYPB gi|63108256|dbj|BAD98250.1| stylopine
           synthase [Eschscholzia californica]
          Length = 495

 Score =  115 bits (288), Expect = 1e-23
 Identities = 60/107 (56%), Positives = 76/107 (71%)
 Frame = -2

Query: 323 NDFWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVA 144
           N+ W++  T   +   + F+F    F + ++   WPAGPKTLPIIGN+H LGG   LQV 
Sbjct: 10  NNPWILTATATTLLISI-FLF----FTRKSSKMVWPAGPKTLPIIGNMHLLGG-TALQVV 63

Query: 143 LANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           L NLAKV+G   TIWIGSW P+IV+SDI+ A EVLVNKS+DYSARD+
Sbjct: 64  LHNLAKVHGSVMTIWIGSWRPVIVVSDIERAWEVLVNKSSDYSARDM 110


>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  114 bits (285), Expect = 3e-23
 Identities = 59/109 (54%), Positives = 77/109 (70%)
 Frame = -2

Query: 332 IEGNDFWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPL 153
           +EG+  W+I  TV IV  +   +  ++S   S++  +WP+GPK LPIIGNLH LGG + L
Sbjct: 1   MEGSFLWLIGSTVAIVLVITTIV--RMSSSSSSSKKKWPSGPKRLPIIGNLHLLGGDL-L 57

Query: 152 QVALANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARD 6
            V LA LAKV+G   TIWIG W P+IVISDI++A EVLV+KSA+Y  RD
Sbjct: 58  HVTLAKLAKVHGSVMTIWIGRWRPIIVISDINSAWEVLVSKSAEYGQRD 106


>gb|AHF65153.1| stylopine synthase, partial [synthetic construct]
          Length = 511

 Score =  112 bits (281), Expect = 8e-23
 Identities = 54/80 (67%), Positives = 64/80 (80%)
 Frame = -2

Query: 242 QSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVALANLAKVYGGAFTIWIGSWVPMIVISD 63
           + ++T  WP GPKTLPIIGN+H LGG   LQV L NLAK++G   TIWIGSW P+IV+SD
Sbjct: 46  KKSSTMVWPTGPKTLPIIGNMHVLGG-TALQVVLHNLAKIHGNVMTIWIGSWRPVIVVSD 104

Query: 62  IDNAREVLVNKSADYSARDV 3
           ID A EVLVNKS+DYSARD+
Sbjct: 105 IDRAWEVLVNKSSDYSARDM 124


>dbj|BAG75116.1| cytochrome P450 [Eschscholzia californica]
          Length = 495

 Score =  111 bits (278), Expect = 2e-22
 Identities = 58/107 (54%), Positives = 75/107 (70%)
 Frame = -2

Query: 323 NDFWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVA 144
           N+ W++  T   +  +  F+F    F + ++   WP GPKTLPIIGN+H LGG   LQV 
Sbjct: 10  NNPWILTATATTLL-ISMFLF----FTRKSSKMVWPQGPKTLPIIGNMHLLGG-TALQVV 63

Query: 143 LANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           L NLAKV+G   TIWIGSW P+IV+SDI+ A EVLVNKS+DYSAR++
Sbjct: 64  LHNLAKVHGSVMTIWIGSWRPVIVVSDIERAWEVLVNKSSDYSAREL 110


>sp|Q50LH4.1|C7193_ESCCA RecName: Full=(S)-stylopine synthase 2; Short=STS; AltName:
           Full=Cytochrome P450 719A3; Short=EcCYP719A3;
           Short=EcCYPA gi|63108254|dbj|BAD98249.1| stylopine
           synthase [Eschscholzia californica]
          Length = 495

 Score =  110 bits (276), Expect = 3e-22
 Identities = 59/107 (55%), Positives = 74/107 (69%)
 Frame = -2

Query: 323 NDFWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVA 144
           N+ W++  T   +   + F+F    F + +    WP GPKTLPIIGN+H LGG   LQV 
Sbjct: 10  NNPWVLFATSATLLISI-FLF----FRRKSPNMAWPEGPKTLPIIGNMHLLGG-TALQVV 63

Query: 143 LANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           L NLAKV+G   TIWIGSW P+IV+SDI+ A EVLVNKS+DYSARD+
Sbjct: 64  LYNLAKVHGRVMTIWIGSWRPVIVVSDIEQAWEVLVNKSSDYSARDM 110


>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  110 bits (274), Expect = 5e-22
 Identities = 58/103 (56%), Positives = 72/103 (69%)
 Frame = -2

Query: 311 MIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPLQVALANL 132
           +   T+ I+  +   +    S   S +   WP+GPK LPIIGNLHQL G + L V LA L
Sbjct: 19  LAGATLAILLTISTIVRITSSPSSSFSQMTWPSGPKKLPIIGNLHQLRGEL-LHVTLAKL 77

Query: 131 AKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDV 3
           AKV+GG  TIW+GSWVP+IVISDI++A EVLVNKSADY AR+V
Sbjct: 78  AKVHGGVMTIWMGSWVPIIVISDINSAWEVLVNKSADYGAREV 120