BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver30_contig00028580
         (2623 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_010263294.1| PREDICTED: isoamylase 1, chloroplastic [Nelu...   694   0.0  
ref|XP_008223817.1| PREDICTED: isoamylase 1, chloroplastic [Prun...   688   0.0  
ref|XP_002265964.3| PREDICTED: isoamylase 1, chloroplastic [Viti...   686   0.0  
ref|XP_009341737.1| PREDICTED: isoamylase 1, chloroplastic [Pyru...   684   0.0  
ref|XP_007014466.1| Isoamylase 1 isoform 2 [Theobroma cacao] gi|...   684   0.0  
ref|XP_007225082.1| hypothetical protein PRUPE_ppa025097mg, part...   682   0.0  
ref|XP_007160229.1| hypothetical protein PHAVU_002G303600g [Phas...   682   0.0  
ref|XP_012075534.1| PREDICTED: isoamylase 1, chloroplastic [Jatr...   681   0.0  
gb|KOM29811.1| hypothetical protein LR48_Vigan818s000900 [Vigna ...   680   0.0  
emb|CBI40669.3| unnamed protein product [Vitis vinifera]              680   0.0  
ref|XP_003532455.2| PREDICTED: isoamylase 1, chloroplastic-like ...   679   0.0  
ref|XP_014510281.1| PREDICTED: isoamylase 1, chloroplastic [Vign...   677   0.0  
ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like ...   677   0.0  
gb|KDO61988.1| hypothetical protein CISIN_1g003653mg [Citrus sin...   674   0.0  
ref|XP_010048547.1| PREDICTED: isoamylase 1, chloroplastic isofo...   674   0.0  
ref|XP_003630623.2| isoamylase-type starch debranching enzyme [M...   672   0.0  
ref|XP_002529900.1| isoamylase, putative [Ricinus communis] gi|2...   671   0.0  
ref|XP_010106957.1| Isoamylase 1 [Morus notabilis] gi|587925578|...   668   0.0  
ref|XP_004503543.1| PREDICTED: isoamylase 1, chloroplastic isofo...   668   0.0  
ref|XP_004503542.1| PREDICTED: isoamylase 1, chloroplastic isofo...   668   0.0  

>ref|XP_010263294.1| PREDICTED: isoamylase 1, chloroplastic [Nelumbo nucifera]
          Length = 806

 Score =  694 bits (1791), Expect(2) = 0.0
 Identities = 314/359 (87%), Positives = 341/359 (94%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDF+DMLYGYKFDGKFSP+EGHYY+SSRI++DPYAKAVISRGEYGA GP+G+CWPQMAG+
Sbjct: 158  GDFEDMLYGYKFDGKFSPEEGHYYDSSRILLDPYAKAVISRGEYGALGPEGSCWPQMAGI 217

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            +PSSD EFDWEGDLPL+H Q+DLIIYEMHVRGFT H+SS +  PGTY+GVVEKLDHLKEL
Sbjct: 218  IPSSDNEFDWEGDLPLKHPQRDLIIYEMHVRGFTMHDSSKSRFPGTYIGVVEKLDHLKEL 277

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            GINCIELMPCQEYNELEYFSYNSVLG+YK+NFWGYSTVNYFSPMIRYSS GI NCG DAI
Sbjct: 278  GINCIELMPCQEYNELEYFSYNSVLGDYKLNFWGYSTVNYFSPMIRYSSGGILNCGRDAI 337

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NEVK L+REAHKRGIEV++DVVFNHTAEGNENGPVLSFRG+DN+ YYMLAPKGEFYNYSG
Sbjct: 338  NEVKTLIREAHKRGIEVLLDVVFNHTAEGNENGPVLSFRGVDNSAYYMLAPKGEFYNYSG 397

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV RQFILDCLRYWVTE+H+DGFRFDLASI+TR  SLWDAVNV+GNPIEGD
Sbjct: 398  CGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASILTRGCSLWDAVNVYGNPIEGD 457

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL +PPLIDM+SNDPIL GVKLIAEAWDTGGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 458  LLTTGTPLGNPPLIDMISNDPILCGVKLIAEAWDTGGLYQVGTFPHWGIWSEWNGKYRD 516



 Score =  440 bits (1131), Expect(2) = 0.0
 Identities = 205/249 (82%), Positives = 219/249 (87%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQE-GRKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQE GRKPWNSINFICAHDGFTL DLVTYN KHNLA            NSWNCGQ
Sbjct: 538  GSPNLYQERGRKPWNSINFICAHDGFTLADLVTYNNKHNLANGEDNNDGENHNNSWNCGQ 597

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEF   PVK+LRKRQMRNFFLCLMVSQGVPM YMGDEYGHTKGGNNNTYCHDNYINYFR
Sbjct: 598  EGEFASLPVKRLRKRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFR 657

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+SSDF+RFC L+T FR ECESLGLNDFPT+ERLQWHGHAP  P+WS+TSRFVAF
Sbjct: 658  WDKKEESSSDFFRFCRLMTTFRRECESLGLNDFPTAERLQWHGHAPRMPDWSDTSRFVAF 717

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            SLVD+VKGEIYVAFNTSHLPVMVTLPER GYRW PLVDTSK +PFDFLS+D+ E+D+AIK
Sbjct: 718  SLVDAVKGEIYVAFNTSHLPVMVTLPERPGYRWEPLVDTSKPSPFDFLSEDLPERDVAIK 777

Query: 508  QYAQFLDGN 482
            QY+ FLD N
Sbjct: 778  QYSHFLDAN 786



 Score = 78.6 bits (192), Expect = 3e-11
 Identities = 39/61 (63%), Positives = 46/61 (75%)
 Frame = -1

Query: 2518 EMVVETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQ 2339
            E+    VEKP       FEVF+GSPMP+GAT ++GGVNFA++SG A S TLCLISLSDLQ
Sbjct: 75   ELETAVVEKPRL---RRFEVFAGSPMPFGATARDGGVNFAIYSGHATSATLCLISLSDLQ 131

Query: 2338 E 2336
            E
Sbjct: 132  E 132


>ref|XP_008223817.1| PREDICTED: isoamylase 1, chloroplastic [Prunus mume]
          Length = 805

 Score =  688 bits (1775), Expect(3) = 0.0
 Identities = 312/359 (86%), Positives = 337/359 (93%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKD LYGYKF+GKFSP+EG YY+SS+IV+DPYAKAVI RGEYG   PDGNCWPQMAG 
Sbjct: 158  GDFKDTLYGYKFEGKFSPEEGLYYDSSKIVLDPYAKAVIRRGEYGKLSPDGNCWPQMAGT 217

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VPS D EFDWEGDLPL++ QKDLIIYE+HVRGFTRHESSM   PGTYLG+VEKLDHLKEL
Sbjct: 218  VPSFDDEFDWEGDLPLKYPQKDLIIYEIHVRGFTRHESSMAELPGTYLGLVEKLDHLKEL 277

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            GINCIELMPC E+NELEYF YNSVLG+YK+NFWGYSTVNYFSPMIRYSSAGIRNCGHDAI
Sbjct: 278  GINCIELMPCHEFNELEYFGYNSVLGDYKVNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 337

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NEVKFL++EAHKRGIEVIMDVVFNHTAEGNENGP+LSFRG DN++YYMLAPKGEFYNYSG
Sbjct: 338  NEVKFLIKEAHKRGIEVIMDVVFNHTAEGNENGPILSFRGADNSIYYMLAPKGEFYNYSG 397

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV RQFI+DCLRYWVTE+H+DGFRFDLASIMTR SSLWDA NV+G PI+GD
Sbjct: 398  CGNTFNCNHPVVRQFIIDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDATNVYGIPIKGD 457

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL +PPL+DM+SNDPILRGVKLIAEAWDTGGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 458  LLTTGTPLANPPLVDMISNDPILRGVKLIAEAWDTGGLYQVGMFPHWGIWSEWNGKYRD 516



 Score =  423 bits (1087), Expect(3) = 0.0
 Identities = 194/249 (77%), Positives = 212/249 (85%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQEG R+PWNSINF+CAHDGFTL DLVTYN KHNLA            NSWNCGQ
Sbjct: 538  GSPNLYQEGGRRPWNSINFVCAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQ 597

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEF    VKKLRKRQM+NFF+CLMVSQGVPM YMGDEYGHTKGGNNNTYCHDNYINYFR
Sbjct: 598  EGEFASISVKKLRKRQMQNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFR 657

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+S DF+RFCCL+TKFR E ESLGLNDFPT+ERLQWHGHAP  P+WSETSRFVAF
Sbjct: 658  WDKKEESSLDFFRFCCLMTKFRQESESLGLNDFPTAERLQWHGHAPGVPDWSETSRFVAF 717

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            +L+DSVK E+Y+AFN SHLP  +TLPER GYRW PLVDTSK APFDFLS D+  +D+A+K
Sbjct: 718  TLMDSVKRELYIAFNASHLPATITLPERPGYRWDPLVDTSKPAPFDFLSSDLPGRDIAVK 777

Query: 508  QYAQFLDGN 482
            QYA FLD N
Sbjct: 778  QYAHFLDAN 786



 Score = 65.5 bits (158), Expect(3) = 0.0
 Identities = 36/61 (59%), Positives = 44/61 (72%), Gaps = 2/61 (3%)
 Frame = -1

Query: 2512 VVET--VEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQ 2339
            VVET  V++P       F+V  G P P+GAT  +GGVNFAV+S  AVSVTLCLI+LSDL+
Sbjct: 75   VVETAVVDRPKL---RRFQVSQGHPSPFGATTCDGGVNFAVYSANAVSVTLCLITLSDLE 131

Query: 2338 E 2336
            E
Sbjct: 132  E 132


>ref|XP_002265964.3| PREDICTED: isoamylase 1, chloroplastic [Vitis vinifera]
          Length = 793

 Score =  686 bits (1771), Expect(3) = 0.0
 Identities = 307/359 (85%), Positives = 339/359 (94%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            G+F++++YGYKFDGKFSP+EGHYY+SSR+++DPYAKAVISRGE+G  GP+GNCWP MAGM
Sbjct: 144  GNFENIVYGYKFDGKFSPEEGHYYDSSRLLLDPYAKAVISRGEFGILGPEGNCWPLMAGM 203

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            +PSSD EFDWEGDLPL++ QKDLIIYEMHVRGFTRHESS T  PGTY GVVEKLDHLKEL
Sbjct: 204  IPSSDAEFDWEGDLPLKYPQKDLIIYEMHVRGFTRHESSRTKFPGTYHGVVEKLDHLKEL 263

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            G+NCIELMPC E+NELEYFSYNSVL +Y++NFWGYSTVNYFSPMIRYSSAGI NCGHDAI
Sbjct: 264  GVNCIELMPCHEFNELEYFSYNSVLDDYRVNFWGYSTVNYFSPMIRYSSAGIHNCGHDAI 323

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NEVK L+REAHKRGIEV+MDVVFNHTAEGNENGP+LSFRG+DN+VYYMLAPKGEFYNYSG
Sbjct: 324  NEVKLLIREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSVYYMLAPKGEFYNYSG 383

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHP+ RQFILDCLRYWVTE+H+DGFRFDLASIMTR SSLWDA+NV+GNP EGD
Sbjct: 384  CGNTFNCNHPIVRQFILDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDALNVYGNPAEGD 443

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
             LTTGTPL SPPLIDM+SNDPILRGVKLIAEAWD GGLYQVG FPHWG+WSEWNGKYRD
Sbjct: 444  SLTTGTPLSSPPLIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGLWSEWNGKYRD 502



 Score =  425 bits (1093), Expect(3) = 0.0
 Identities = 195/249 (78%), Positives = 215/249 (86%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQEG RKPWNSINF+CAHDGFTL DLVTYN+KHN A            NSWNCGQ
Sbjct: 524  GSPNLYQEGGRKPWNSINFVCAHDGFTLADLVTYNKKHNNANGEDNNDGENHNNSWNCGQ 583

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEF    VKKLRKRQMRNFFLCLMVSQGVPM YMGDEYGHTKGGNNNTYC DNY+NYFR
Sbjct: 584  EGEFASISVKKLRKRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYCQDNYMNYFR 643

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+ SDF+RFCCL++KFR ECESLGLNDFPT+ERLQWHG  P  P+WS+TSRFVAF
Sbjct: 644  WDKKEESLSDFFRFCCLMSKFRQECESLGLNDFPTAERLQWHGRTPGMPDWSKTSRFVAF 703

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            ++VDSVKGEIYVAFNTSHLP+++TLPER GYRW PLVDTSK APFDFLS+DV E+D A+K
Sbjct: 704  TMVDSVKGEIYVAFNTSHLPIIITLPERPGYRWQPLVDTSKPAPFDFLSNDVPERDTAVK 763

Query: 508  QYAQFLDGN 482
            QY+QF + N
Sbjct: 764  QYSQFTEAN 772



 Score = 68.6 bits (166), Expect(3) = 0.0
 Identities = 37/62 (59%), Positives = 41/62 (66%)
 Frame = -1

Query: 2521 AEMVVETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDL 2342
            AE     VEKP       F VF G P P GAT ++GGVNFAV+SG AVS TLCLIS SDL
Sbjct: 60   AEAETVVVEKPKL---QPFLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCLISASDL 116

Query: 2341 QE 2336
            +E
Sbjct: 117  EE 118


>ref|XP_009341737.1| PREDICTED: isoamylase 1, chloroplastic [Pyrus x bretschneideri]
          Length = 801

 Score =  684 bits (1766), Expect(3) = 0.0
 Identities = 310/359 (86%), Positives = 336/359 (93%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKD LYGYKFDGKFSP+EGHYY+SSR+V+DPYAKAVI RGE+   GPDGNCWPQMAG 
Sbjct: 154  GDFKDTLYGYKFDGKFSPEEGHYYDSSRVVLDPYAKAVIRRGEFRKLGPDGNCWPQMAGT 213

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VPS D +FDWEGDLPL++ QKDLIIYEMHVRGFTRHESS T  PGTYLG+V+KLDHLKEL
Sbjct: 214  VPSFDDQFDWEGDLPLKYPQKDLIIYEMHVRGFTRHESSATEFPGTYLGLVDKLDHLKEL 273

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            GINC+ELMPC E+NELEYFSYNSVLG+YK+NFWGYSTVNYFSPMIRYSSAGIRNCG DAI
Sbjct: 274  GINCLELMPCHEFNELEYFSYNSVLGDYKVNFWGYSTVNYFSPMIRYSSAGIRNCGRDAI 333

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NEVKFL+REAHKRGIEVIMDVVFNHTAEGNENGP+LSFRG DN+VYYMLAPKGEFYNYSG
Sbjct: 334  NEVKFLIREAHKRGIEVIMDVVFNHTAEGNENGPILSFRGADNSVYYMLAPKGEFYNYSG 393

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV RQFI+DCLRYWVTE+H+DGFRFDLASIMTR SSLWDA+NV+G+ IEGD
Sbjct: 394  CGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDAINVYGSAIEGD 453

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL +PPL+DM+SNDPIL GVKLIAEAWD GGLYQVG FPHWG WSEWNGKYRD
Sbjct: 454  LLTTGTPLATPPLVDMISNDPILHGVKLIAEAWDAGGLYQVGMFPHWGNWSEWNGKYRD 512



 Score =  425 bits (1093), Expect(3) = 0.0
 Identities = 193/248 (77%), Positives = 211/248 (85%)
 Frame = -1

Query: 1225 GSPNLYQEGRKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQE 1046
            GSPNLYQEG +PW+S+NF+CAHDGFTL DLVTYN KHNLA            NSWNCGQE
Sbjct: 534  GSPNLYQEGGRPWSSVNFVCAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQE 593

Query: 1045 GEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFRW 866
            GEF    VKKLRKRQMRNFF+CLMVSQGVPM  MGDEYGHTKGGNNNTYCHDNYINYFRW
Sbjct: 594  GEFASISVKKLRKRQMRNFFVCLMVSQGVPMLCMGDEYGHTKGGNNNTYCHDNYINYFRW 653

Query: 865  DKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAFS 686
            DKKEE+SSDF+RFCCL+TKFR ECESLGLNDFPT+ERLQWHGHAP  P+WS+TSRFVAF+
Sbjct: 654  DKKEESSSDFFRFCCLMTKFRQECESLGLNDFPTAERLQWHGHAPGVPDWSDTSRFVAFT 713

Query: 685  LVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIKQ 506
            LVDSVK E+Y+AFN SHL V ++LPER GYRW PLVDTSK  PFDFLS DV E+D A+KQ
Sbjct: 714  LVDSVKRELYIAFNASHLAVTISLPERPGYRWEPLVDTSKCTPFDFLSSDVPERDTAVKQ 773

Query: 505  YAQFLDGN 482
            YA FLD N
Sbjct: 774  YAHFLDAN 781



 Score = 68.2 bits (165), Expect(3) = 0.0
 Identities = 31/44 (70%), Positives = 38/44 (86%)
 Frame = -1

Query: 2467 FEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQE 2336
            F+V  G P P+GAT ++GGVNFAV+SG AVSVTLCLI+LSDL+E
Sbjct: 85   FQVSDGYPSPFGATARDGGVNFAVYSGNAVSVTLCLIALSDLEE 128


>ref|XP_007014466.1| Isoamylase 1 isoform 2 [Theobroma cacao] gi|508784829|gb|EOY32085.1|
            Isoamylase 1 isoform 2 [Theobroma cacao]
          Length = 795

 Score =  684 bits (1765), Expect(2) = 0.0
 Identities = 305/359 (84%), Positives = 340/359 (94%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKDMLYGY FDGKFSP++GHYY+SS+I++DPYAKAVISRGE+GA GP+ NCWPQMA M
Sbjct: 147  GDFKDMLYGYIFDGKFSPEKGHYYDSSKILLDPYAKAVISRGEFGALGPEDNCWPQMACM 206

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VP+S+ EFDWEGDLPLR+ Q+DLIIYEMHVRGFTRHESS T  PGTY GVVEKLDHLKEL
Sbjct: 207  VPTSEDEFDWEGDLPLRYSQRDLIIYEMHVRGFTRHESSRTEFPGTYRGVVEKLDHLKEL 266

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            G+NCIELMPC E+NELEY+SYNS+LG+YK+NFWGYSTVN+FSPMIRYSS+G+ NCG DA+
Sbjct: 267  GVNCIELMPCHEFNELEYYSYNSILGDYKLNFWGYSTVNFFSPMIRYSSSGMHNCGRDAV 326

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE K+LV+EAHKRGIEV MDVVFNHTAEGNENGP+LSFRG+DN+VYYMLAPKGE+YNYSG
Sbjct: 327  NEFKYLVKEAHKRGIEVFMDVVFNHTAEGNENGPILSFRGVDNSVYYMLAPKGEYYNYSG 386

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV RQFILDCLRYWVTE+H+DGFRFDLASIMTRSSSLWD VNVFGNP++GD
Sbjct: 387  CGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDLVNVFGNPLKGD 446

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            L+TTGTPL SPPLIDM+SNDP+LRGVKLIAEAWDTGGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 447  LITTGTPLSSPPLIDMMSNDPVLRGVKLIAEAWDTGGLYQVGSFPHWGIWSEWNGKYRD 505



 Score =  429 bits (1103), Expect(2) = 0.0
 Identities = 197/249 (79%), Positives = 214/249 (85%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQEG RKPWNSINF+CAHDGFTL DLVTYN KHNLA            NSWNCGQ
Sbjct: 527  GSPNLYQEGGRKPWNSINFVCAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQ 586

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEF    VKKLRKRQMRNFFLCLMVSQG+PM  MGDEYGHTKGGNNNTYCHDNYINYF+
Sbjct: 587  EGEFASISVKKLRKRQMRNFFLCLMVSQGIPMICMGDEYGHTKGGNNNTYCHDNYINYFQ 646

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE SSDF+RFCCL+TKFR ECESLGLNDFPT+ERLQWHGH P  P+WS+ SRFVAF
Sbjct: 647  WDKKEECSSDFFRFCCLMTKFRRECESLGLNDFPTAERLQWHGHTPGMPDWSDRSRFVAF 706

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            +L+DSVKGEIYVAFNTS+LPV +TLPE  GYRW PLVDTSK APFDFLS+++ E+D+AIK
Sbjct: 707  TLIDSVKGEIYVAFNTSYLPVTITLPEHPGYRWEPLVDTSKPAPFDFLSEELKERDIAIK 766

Query: 508  QYAQFLDGN 482
            QY QFLD N
Sbjct: 767  QYVQFLDAN 775



 Score = 63.2 bits (152), Expect = 1e-06
 Identities = 32/62 (51%), Positives = 41/62 (66%)
 Frame = -1

Query: 2521 AEMVVETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDL 2342
            AE     VEKP       F++  G P P+GAT+ +GGVNFA++S  AVS TLCLI+ SDL
Sbjct: 63   AEADTAVVEKPK---IKRFQLSEGHPGPFGATLLDGGVNFAIYSANAVSATLCLITFSDL 119

Query: 2341 QE 2336
            Q+
Sbjct: 120  QQ 121


>ref|XP_007225082.1| hypothetical protein PRUPE_ppa025097mg, partial [Prunus persica]
            gi|462422018|gb|EMJ26281.1| hypothetical protein
            PRUPE_ppa025097mg, partial [Prunus persica]
          Length = 777

 Score =  682 bits (1759), Expect(3) = 0.0
 Identities = 311/359 (86%), Positives = 334/359 (93%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKD LYGYKF+GKFSP+EG YY+SS+IV+DPYAKAVI RGEYG   PDGNCWPQMAG 
Sbjct: 104  GDFKDTLYGYKFEGKFSPEEGLYYDSSKIVLDPYAKAVIRRGEYGKLSPDGNCWPQMAGT 163

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VPS D EFDWEGDLPL + QKDLIIYE+HVRGFTRHESSM   PGTYLG+VEKLDHLKEL
Sbjct: 164  VPSFDDEFDWEGDLPLNYPQKDLIIYEIHVRGFTRHESSMAELPGTYLGLVEKLDHLKEL 223

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            GINCIELMPC E+NELEYF YNSVLG+YK+NFWGYSTVNYFSPMIRYSSAGIRNCG DAI
Sbjct: 224  GINCIELMPCHEFNELEYFGYNSVLGDYKVNFWGYSTVNYFSPMIRYSSAGIRNCGRDAI 283

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NEVKFL++EAHKRGIEVIMDVVFNHTAEGNENGP+LSFRG DN++YYMLA KGEFYNYSG
Sbjct: 284  NEVKFLIKEAHKRGIEVIMDVVFNHTAEGNENGPILSFRGADNSIYYMLAHKGEFYNYSG 343

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV RQFI+DCLRYWVTE+H+DGFRFDLASIMTR SSLWDA NV+G PIEGD
Sbjct: 344  CGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDATNVYGVPIEGD 403

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL +PPL+DM+SNDPILRGVKLIAEAWDTGGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 404  LLTTGTPLANPPLVDMISNDPILRGVKLIAEAWDTGGLYQVGMFPHWGIWSEWNGKYRD 462



 Score =  412 bits (1058), Expect(3) = 0.0
 Identities = 195/275 (70%), Positives = 213/275 (77%), Gaps = 27/275 (9%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQ+G R+PWNSINF+CAHDGFTL DLVTYN KHNLA            NSWNCGQ
Sbjct: 484  GSPNLYQKGGRRPWNSINFVCAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQ 543

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNY----- 884
            EGEF    VKKLRKRQMRNFF+CLMVSQGVPM YMGDEYGHTKGGNNNTYCHDNY     
Sbjct: 544  EGEFARISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYVFFYT 603

Query: 883  ---------------------INYFRWDKKEETSSDFYRFCCLITKFRHECESLGLNDFP 767
                                 INYFRWDKKEE+S DF+RFCCL+TKFR ECESLGLNDFP
Sbjct: 604  FVHTLSVKPFSLFVMVVAVLQINYFRWDKKEESSLDFFRFCCLMTKFRQECESLGLNDFP 663

Query: 766  TSERLQWHGHAPCEPNWSETSRFVAFSLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWT 587
            T+ERLQWHGHAP  P+WSETSRFVAF+L+DSVK E+Y+AFN SHLP  +TLPER GYRW 
Sbjct: 664  TAERLQWHGHAPGVPDWSETSRFVAFTLMDSVKRELYIAFNASHLPATITLPERPGYRWD 723

Query: 586  PLVDTSKAAPFDFLSDDVSEKDLAIKQYAQFLDGN 482
            PLVDTSK APFDFLS D+  +D+A+KQYA FLD N
Sbjct: 724  PLVDTSKPAPFDFLSSDLPGRDIAVKQYAHFLDAN 758



 Score = 67.4 bits (163), Expect(3) = 0.0
 Identities = 36/61 (59%), Positives = 45/61 (73%), Gaps = 2/61 (3%)
 Frame = -1

Query: 2512 VVET--VEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQ 2339
            VVET  V++P       F+V  G P P+GAT ++GGVNFAV+S  AVSVTLCLI+LSDL+
Sbjct: 21   VVETAVVDRPKL---RRFQVSQGHPSPFGATTRDGGVNFAVYSANAVSVTLCLITLSDLE 77

Query: 2338 E 2336
            E
Sbjct: 78   E 78


>ref|XP_007160229.1| hypothetical protein PHAVU_002G303600g [Phaseolus vulgaris]
            gi|139867053|dbj|BAF52941.1| isoamylase-type
            starch-debranching enzyme 1 [Phaseolus vulgaris]
            gi|561033644|gb|ESW32223.1| hypothetical protein
            PHAVU_002G303600g [Phaseolus vulgaris]
          Length = 791

 Score =  682 bits (1759), Expect(3) = 0.0
 Identities = 305/359 (84%), Positives = 338/359 (94%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDF DMLYGYKFDGKFSP EGHYY+SSRIV+DPYAKAVISRGE+GA GP+GNCWPQMAGM
Sbjct: 143  GDFSDMLYGYKFDGKFSPLEGHYYDSSRIVLDPYAKAVISRGEFGALGPNGNCWPQMAGM 202

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VPS   EFDWEGDLPL++ QKDL++YEMHVRGFT+HESS T  PGTYLGVVEKLDHLKEL
Sbjct: 203  VPSDHDEFDWEGDLPLKYPQKDLVVYEMHVRGFTKHESSNTKFPGTYLGVVEKLDHLKEL 262

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            G+NC+ELMPC E+NELEY SYNSV G+Y++NFWGYST+N+FSPMIRYSSAGIRNCG D I
Sbjct: 263  GVNCLELMPCHEFNELEYSSYNSVQGDYRVNFWGYSTINFFSPMIRYSSAGIRNCGRDGI 322

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE+KFL++EAHKRGIEVIMDVVFNHTAEGNE+GP++SFRG+DN++YYM+APKGEFYNYSG
Sbjct: 323  NEIKFLIKEAHKRGIEVIMDVVFNHTAEGNEDGPIISFRGVDNSIYYMVAPKGEFYNYSG 382

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNC+HPV RQFI+DCLRYWVTE+H+DGFRFDLASIMTRSSSLWDA NVFG PIEGD
Sbjct: 383  CGNTFNCSHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDATNVFGAPIEGD 442

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL SPPLID++SNDPILRGVKLIAEAWD GGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 443  LLTTGTPLGSPPLIDLISNDPILRGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYRD 501



 Score =  417 bits (1071), Expect(3) = 0.0
 Identities = 194/249 (77%), Positives = 210/249 (84%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQ G RKPWNSINF+CAHDGFTL DLVTY  KHNL+            NSWNCGQ
Sbjct: 523  GSPNLYQGGGRKPWNSINFVCAHDGFTLADLVTYTNKHNLSNGEDNNDGENHNNSWNCGQ 582

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEFV   VKKLRKRQMRN FL LMVSQGVPM YMGDEYGHTKGGNNNTYCHDNY+NYF+
Sbjct: 583  EGEFVSTSVKKLRKRQMRNLFLSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYLNYFQ 642

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+SSDF+RFCCL+TKFR ECESLGL+DFPTSERLQWHGH P  P+WSETSRFVAF
Sbjct: 643  WDKKEESSSDFFRFCCLVTKFRQECESLGLDDFPTSERLQWHGHFPGMPDWSETSRFVAF 702

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            +LVDSVKGEIYVAFN SHLP  +TLPER GYRW PLVDTSK  PFDFL+ D+  +D+AI+
Sbjct: 703  TLVDSVKGEIYVAFNMSHLPFTITLPERPGYRWEPLVDTSKPIPFDFLTPDLPGRDIAIQ 762

Query: 508  QYAQFLDGN 482
            QYA FLD N
Sbjct: 763  QYAHFLDAN 771



 Score = 59.7 bits (143), Expect(3) = 0.0
 Identities = 31/54 (57%), Positives = 38/54 (70%)
 Frame = -1

Query: 2500 VEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQ 2339
            VEKP   L S F+V  G   P+GATV++GGVNFA++S  A S TLCL +LSD Q
Sbjct: 65   VEKPQ--LESLFQVSRGYASPFGATVRDGGVNFAIYSLNAFSATLCLFTLSDFQ 116


>ref|XP_012075534.1| PREDICTED: isoamylase 1, chloroplastic [Jatropha curcas]
            gi|643726052|gb|KDP34860.1| hypothetical protein
            JCGZ_09148 [Jatropha curcas]
          Length = 793

 Score =  681 bits (1756), Expect(3) = 0.0
 Identities = 308/359 (85%), Positives = 333/359 (92%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKDMLYGY+FDGK SP+EGHY+ SS IV+DPYAKAVISRGE+G  GPD NCWPQMAGM
Sbjct: 145  GDFKDMLYGYRFDGKLSPEEGHYFESSEIVLDPYAKAVISRGEFGVLGPDDNCWPQMAGM 204

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            +P++  +FDWEGDLPL++ QKDL+IYEMHVRGFT+HESS T  PGTYLGVVEKLDHLKEL
Sbjct: 205  IPAARDKFDWEGDLPLKYPQKDLMIYEMHVRGFTQHESSRTEFPGTYLGVVEKLDHLKEL 264

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            G+NCIELMPC E+NELEY+SYNSVLG+YKMNFWGYSTVNYFSPM RYSSAG RNCGHDAI
Sbjct: 265  GVNCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYSTVNYFSPMTRYSSAGTRNCGHDAI 324

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE K LVREAHKRGIEVIMDVVFNHTAEGNE GP+LSFRG+DN+VYYMLAPKGEFYNYSG
Sbjct: 325  NEFKLLVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGVDNSVYYMLAPKGEFYNYSG 384

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHP+ RQFILDCLRYWV E+H+DGFRFDLASIMTR SSLWDAVNVFGNP+EGD
Sbjct: 385  CGNTFNCNHPIVRQFILDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVFGNPVEGD 444

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL SPPLIDM+SNDPILR VKLIAEAWD GGLYQVG FPHW IWSEWNGKYRD
Sbjct: 445  LLTTGTPLSSPPLIDMISNDPILREVKLIAEAWDAGGLYQVGCFPHWQIWSEWNGKYRD 503



 Score =  426 bits (1095), Expect(3) = 0.0
 Identities = 197/249 (79%), Positives = 212/249 (85%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQEG RKPWNSINF+CAHDGFTL DLVTYN K+NLA            NSWNCG 
Sbjct: 525  GSPNLYQEGGRKPWNSINFVCAHDGFTLADLVTYNNKNNLANGEDNNDGENHNNSWNCGL 584

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEF    VKKLRKRQMRNFF+CLMVSQGVPM YMGDEYGHTKGGNNNTYCHDNYINYFR
Sbjct: 585  EGEFASISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFR 644

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+SSDFYRFCCL+TKFR ECESLGLNDFPT+ERLQWHGH P  P+WSETSRFVAF
Sbjct: 645  WDKKEESSSDFYRFCCLMTKFRSECESLGLNDFPTAERLQWHGHVPRMPDWSETSRFVAF 704

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            ++ D+VKGEIYVAFN  HLPV V LPER GYRW PLVDT KA+PFDFLS DV E+++AIK
Sbjct: 705  TVTDTVKGEIYVAFNAKHLPVTVALPERLGYRWEPLVDTGKASPFDFLSGDVPEREIAIK 764

Query: 508  QYAQFLDGN 482
            QY+ FLD N
Sbjct: 765  QYSHFLDAN 773



 Score = 54.7 bits (130), Expect(3) = 0.0
 Identities = 27/53 (50%), Positives = 36/53 (67%)
 Frame = -1

Query: 2500 VEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDL 2342
            V++P   L   ++V  G P P+GAT  + GVNF+V+S  AVS +LCLISL DL
Sbjct: 67   VDQPK--LRRRYQVSEGHPAPFGATASDDGVNFSVYSTNAVSASLCLISLDDL 117


>gb|KOM29811.1| hypothetical protein LR48_Vigan818s000900 [Vigna angularis]
          Length = 790

 Score =  680 bits (1754), Expect(3) = 0.0
 Identities = 304/359 (84%), Positives = 337/359 (93%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDF DMLYGY+FDGKFSP EGHYY+SSRI++DPYAKAVISRGE+GA GP+GNCWPQMAGM
Sbjct: 142  GDFSDMLYGYQFDGKFSPLEGHYYDSSRILLDPYAKAVISRGEFGALGPNGNCWPQMAGM 201

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VPS   EFDWEGDLPL++ QKDL++YEMHVRGFT+HESS T  PGTYLGVVEKLDHLKEL
Sbjct: 202  VPSDHDEFDWEGDLPLKYPQKDLVVYEMHVRGFTKHESSNTKFPGTYLGVVEKLDHLKEL 261

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            G+NC+ELMPC E+NELEY SYNSV G+Y++NFWGYSTVN+FSPMIRYSSAGIRNCG D I
Sbjct: 262  GVNCLELMPCHEFNELEYSSYNSVQGDYRVNFWGYSTVNFFSPMIRYSSAGIRNCGRDGI 321

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE+KFL++EAHKRGIEVIMDVVFNHTAEGNENGP++SFRG+DN++YYM+APKGEFYNYSG
Sbjct: 322  NEIKFLIKEAHKRGIEVIMDVVFNHTAEGNENGPIISFRGVDNSIYYMVAPKGEFYNYSG 381

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNC+HPV RQFI+DCLRYWVTE+H+DGFRFDLASIMTRSSSLWDA NVFG PIEGD
Sbjct: 382  CGNTFNCSHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDATNVFGAPIEGD 441

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
             LTTGTPL SPPLID++SNDPILRGVKLIAEAWD GGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 442  FLTTGTPLGSPPLIDLISNDPILRGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYRD 500



 Score =  420 bits (1080), Expect(3) = 0.0
 Identities = 194/249 (77%), Positives = 215/249 (86%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQ G RKPWNSINF+CAHDGFTL DLVTYN KHNL+            NSWNCGQ
Sbjct: 522  GSPNLYQGGGRKPWNSINFVCAHDGFTLADLVTYNNKHNLSNGEDNNDGENHNNSWNCGQ 581

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEFV   VKKLRKRQMRNFFL LMVSQGVPM YMGDEYGHTKGGNNNTYCHDNY+NYF+
Sbjct: 582  EGEFVSPLVKKLRKRQMRNFFLTLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYLNYFQ 641

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+SSDF+RFCCL+TKFR ECES+GL+DFPTS RLQWHGH P  P+WSETSRFVAF
Sbjct: 642  WDKKEESSSDFFRFCCLVTKFRQECESMGLDDFPTSARLQWHGHFPGMPDWSETSRFVAF 701

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            ++VDSVKGEIYVAFNTSHLP  +TLP+R GYRW PLVDTSKAAP+DFL+ D+  +++AI+
Sbjct: 702  TMVDSVKGEIYVAFNTSHLPFTITLPDRPGYRWEPLVDTSKAAPYDFLTPDLPGREIAIQ 761

Query: 508  QYAQFLDGN 482
            QYAQFLD N
Sbjct: 762  QYAQFLDAN 770



 Score = 66.2 bits (160), Expect(3) = 0.0
 Identities = 33/57 (57%), Positives = 42/57 (73%)
 Frame = -1

Query: 2509 VETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQ 2339
            +  VEKP   L S F+V SG P P+GATV++GGVNFA++S  A+S TLCL +LSD Q
Sbjct: 61   IAVVEKPQ--LESLFQVSSGYPSPFGATVRDGGVNFAIYSLNALSATLCLFTLSDFQ 115


>emb|CBI40669.3| unnamed protein product [Vitis vinifera]
          Length = 809

 Score =  680 bits (1754), Expect(3) = 0.0
 Identities = 307/365 (84%), Positives = 339/365 (92%), Gaps = 6/365 (1%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            G+F++++YGYKFDGKFSP+EGHYY+SSR+++DPYAKAVISRGE+G  GP+GNCWP MAGM
Sbjct: 154  GNFENIVYGYKFDGKFSPEEGHYYDSSRLLLDPYAKAVISRGEFGILGPEGNCWPLMAGM 213

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            +PSSD EFDWEGDLPL++ QKDLIIYEMHVRGFTRHESS T  PGTY GVVEKLDHLKEL
Sbjct: 214  IPSSDAEFDWEGDLPLKYPQKDLIIYEMHVRGFTRHESSRTKFPGTYHGVVEKLDHLKEL 273

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYK------MNFWGYSTVNYFSPMIRYSSAGIRN 1795
            G+NCIELMPC E+NELEYFSYNSVL +Y+      +NFWGYSTVNYFSPMIRYSSAGI N
Sbjct: 274  GVNCIELMPCHEFNELEYFSYNSVLDDYRYTCISMVNFWGYSTVNYFSPMIRYSSAGIHN 333

Query: 1794 CGHDAINEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGE 1615
            CGHDAINEVK L+REAHKRGIEV+MDVVFNHTAEGNENGP+LSFRG+DN+VYYMLAPKGE
Sbjct: 334  CGHDAINEVKLLIREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSVYYMLAPKGE 393

Query: 1614 FYNYSGCGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFG 1435
            FYNYSGCGNTFNCNHP+ RQFILDCLRYWVTE+H+DGFRFDLASIMTR SSLWDA+NV+G
Sbjct: 394  FYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDALNVYG 453

Query: 1434 NPIEGDLLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWN 1255
            NP EGD LTTGTPL SPPLIDM+SNDPILRGVKLIAEAWD GGLYQVG FPHWG+WSEWN
Sbjct: 454  NPAEGDSLTTGTPLSSPPLIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGLWSEWN 513

Query: 1254 GKYRD 1240
            GKYRD
Sbjct: 514  GKYRD 518



 Score =  425 bits (1093), Expect(3) = 0.0
 Identities = 195/249 (78%), Positives = 215/249 (86%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQEG RKPWNSINF+CAHDGFTL DLVTYN+KHN A            NSWNCGQ
Sbjct: 540  GSPNLYQEGGRKPWNSINFVCAHDGFTLADLVTYNKKHNNANGEDNNDGENHNNSWNCGQ 599

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEF    VKKLRKRQMRNFFLCLMVSQGVPM YMGDEYGHTKGGNNNTYC DNY+NYFR
Sbjct: 600  EGEFASISVKKLRKRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYCQDNYMNYFR 659

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+ SDF+RFCCL++KFR ECESLGLNDFPT+ERLQWHG  P  P+WS+TSRFVAF
Sbjct: 660  WDKKEESLSDFFRFCCLMSKFRQECESLGLNDFPTAERLQWHGRTPGMPDWSKTSRFVAF 719

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            ++VDSVKGEIYVAFNTSHLP+++TLPER GYRW PLVDTSK APFDFLS+DV E+D A+K
Sbjct: 720  TMVDSVKGEIYVAFNTSHLPIIITLPERPGYRWQPLVDTSKPAPFDFLSNDVPERDTAVK 779

Query: 508  QYAQFLDGN 482
            QY+QF + N
Sbjct: 780  QYSQFTEAN 788



 Score = 68.6 bits (166), Expect(3) = 0.0
 Identities = 37/62 (59%), Positives = 41/62 (66%)
 Frame = -1

Query: 2521 AEMVVETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDL 2342
            AE     VEKP       F VF G P P GAT ++GGVNFAV+SG AVS TLCLIS SDL
Sbjct: 70   AEAETVVVEKPKL---QPFLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCLISASDL 126

Query: 2341 QE 2336
            +E
Sbjct: 127  EE 128


>ref|XP_003532455.2| PREDICTED: isoamylase 1, chloroplastic-like [Glycine max]
            gi|734363630|gb|KHN16691.1| Isoamylase 1, chloroplastic
            [Glycine soja] gi|947092828|gb|KRH41413.1| hypothetical
            protein GLYMA_08G028400 [Glycine max]
          Length = 798

 Score =  679 bits (1752), Expect(3) = 0.0
 Identities = 303/359 (84%), Positives = 335/359 (93%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDF+DMLYGYKFDGKFSP EGHY++SS I++DPYAKAVISRGE+GA GPDGNCWPQMAG 
Sbjct: 150  GDFRDMLYGYKFDGKFSPLEGHYFDSSHILLDPYAKAVISRGEFGALGPDGNCWPQMAGT 209

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VPS D EFDWEGDLPL++ QKDL+IYEMHVRGFT+HESS T  PGTYLGVVEKLDHLKEL
Sbjct: 210  VPSEDDEFDWEGDLPLKYPQKDLVIYEMHVRGFTKHESSNTKFPGTYLGVVEKLDHLKEL 269

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            G+NC+ELMPC E+NELEY+ +NS  G+Y++NFWGYST+NYFSPMIRYSSAGIRNCG D I
Sbjct: 270  GVNCLELMPCHEFNELEYYGHNSAQGDYRVNFWGYSTINYFSPMIRYSSAGIRNCGQDGI 329

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE+KFL++EAHKRGIEVIMDVVFNHTAEGNENGP++SFRG+DN++YYMLAPKGEFYNYSG
Sbjct: 330  NEIKFLIKEAHKRGIEVIMDVVFNHTAEGNENGPIISFRGVDNSMYYMLAPKGEFYNYSG 389

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV RQFI+DCLRYWVTE+H+DGFRFDLASIMTRSSSLWD  NVFG PIEGD
Sbjct: 390  CGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDGANVFGAPIEGD 449

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL SPPLID++SNDPIL GVKLIAEAWD GGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 450  LLTTGTPLSSPPLIDLISNDPILCGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYRD 508



 Score =  405 bits (1042), Expect(3) = 0.0
 Identities = 190/249 (76%), Positives = 210/249 (84%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQ G RKPW+SINF+CAHDGFTL DLVTYN K+NL+            NSWNCGQ
Sbjct: 530  GSPNLYQGGGRKPWHSINFVCAHDGFTLADLVTYNNKNNLSNGEDNNDGENHNNSWNCGQ 589

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEFV   VKKLRKRQMRNFFL LMVSQGVPM YMGDEYGHTKGGNNNTYCHDNY NYF+
Sbjct: 590  EGEFVSTSVKKLRKRQMRNFFLSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYHNYFQ 649

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+SSDF+RFC L+TKFR ECESLGL DFPTSERLQWHGH P +P+WSETSRFVA 
Sbjct: 650  WDKKEESSSDFFRFCRLMTKFRQECESLGLADFPTSERLQWHGHFPGKPDWSETSRFVAC 709

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            ++VDSVKGEIY+AFN SHLP  VTLPER GY+W PLVDTSK  P+DFL+ D+  +D+AI+
Sbjct: 710  TMVDSVKGEIYIAFNMSHLPFTVTLPERPGYKWEPLVDTSKPTPYDFLTPDLPGRDIAIQ 769

Query: 508  QYAQFLDGN 482
            QYAQFLD N
Sbjct: 770  QYAQFLDAN 778



 Score = 57.8 bits (138), Expect(3) = 0.0
 Identities = 30/60 (50%), Positives = 38/60 (63%)
 Frame = -1

Query: 2518 EMVVETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQ 2339
            E     VEKP   L + F+   G P P+GATV++GGVNFA+ S  A+S TLC  +LSD Q
Sbjct: 66   ETETAVVEKPQ--LKTLFQASRGYPSPFGATVRDGGVNFAISSLNALSATLCFFTLSDFQ 123


>ref|XP_014510281.1| PREDICTED: isoamylase 1, chloroplastic [Vigna radiata var. radiata]
          Length = 790

 Score =  677 bits (1746), Expect(3) = 0.0
 Identities = 304/359 (84%), Positives = 336/359 (93%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDF DMLYGYKFDGKFSP EG YY+ SRI++DPYAKAVISRGE+GA GP+GNCWPQMAGM
Sbjct: 142  GDFTDMLYGYKFDGKFSPLEGQYYDPSRILLDPYAKAVISRGEFGALGPNGNCWPQMAGM 201

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VPS   EFDWEGDLPL++ QKDL++YEMHVRGFT+HESS T  PGTYLGVVEKLDHLKEL
Sbjct: 202  VPSDHDEFDWEGDLPLKYPQKDLVVYEMHVRGFTKHESSNTKFPGTYLGVVEKLDHLKEL 261

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            G+NC+ELMPC E+NELEY SYNSV G+Y++NFWGYSTVN+FSPMIRYSSAGIRNCG D I
Sbjct: 262  GVNCLELMPCHEFNELEYSSYNSVQGDYRVNFWGYSTVNFFSPMIRYSSAGIRNCGRDGI 321

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE+KFL++EAHKRGIEVIMDVVFNHTAEGNENGP++SFRG+DN++YYM+APKGEFYNYSG
Sbjct: 322  NEMKFLIKEAHKRGIEVIMDVVFNHTAEGNENGPIISFRGVDNSIYYMVAPKGEFYNYSG 381

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNC+HPV RQFI+DCLRYWVTE+H+DGFRFDLASIMTRSSSLWDA NVFG PIEGD
Sbjct: 382  CGNTFNCSHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDATNVFGAPIEGD 441

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL SPPLID++SNDPILRGVKLIAEAWD GGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 442  LLTTGTPLGSPPLIDLISNDPILRGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYRD 500



 Score =  420 bits (1079), Expect(3) = 0.0
 Identities = 193/249 (77%), Positives = 214/249 (85%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQ G RKPWNSINF+CAHDGFTL DLVTYN KHNL+            NSWNCGQ
Sbjct: 522  GSPNLYQGGGRKPWNSINFVCAHDGFTLADLVTYNNKHNLSNGEDNNDGENHNNSWNCGQ 581

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEFV   VKKLRKRQMRNFFL LMVSQGVPM YMGDEYGHTKGGNNNTYCHDNY+NYF+
Sbjct: 582  EGEFVSPLVKKLRKRQMRNFFLSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYLNYFQ 641

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+SSDF+RFCCL+TKFR ECES+GL+DFPTSERLQWHGH P  P+WSETSRFVAF
Sbjct: 642  WDKKEESSSDFFRFCCLVTKFRQECESMGLDDFPTSERLQWHGHFPGMPDWSETSRFVAF 701

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            ++ DSVKGEIYVAFNTSHLP  +TLPER GYRW PL+DTSK AP+DFL+ D+  +++AI+
Sbjct: 702  TMADSVKGEIYVAFNTSHLPFTITLPERPGYRWEPLIDTSKPAPYDFLTPDLPGREIAIQ 761

Query: 508  QYAQFLDGN 482
            QYAQFLD N
Sbjct: 762  QYAQFLDAN 770



 Score = 66.2 bits (160), Expect(3) = 0.0
 Identities = 33/57 (57%), Positives = 42/57 (73%)
 Frame = -1

Query: 2509 VETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQ 2339
            +  VEKP   L S F+V SG P P+GATV++GGVNFA++S  A+S TLCL +LSD Q
Sbjct: 61   IAVVEKPQ--LESLFQVSSGYPSPFGATVRDGGVNFAIYSLNALSATLCLFTLSDFQ 115


>ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like [Citrus sinensis]
          Length = 805

 Score =  677 bits (1746), Expect(3) = 0.0
 Identities = 304/359 (84%), Positives = 336/359 (93%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKDMLYGYKFDGKFSP EGHY++ ++IV+DPYAKAVISR ++G  GPD NCWPQMA +
Sbjct: 157  GDFKDMLYGYKFDGKFSPQEGHYFDPTKIVLDPYAKAVISRAQFGVLGPDENCWPQMACL 216

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VP+ + EFDWEGDLPL++ Q+DLIIYE+HVRGFTRHESS T  PGTYLGVVEKLDHLK+L
Sbjct: 217  VPTPEDEFDWEGDLPLKYPQRDLIIYEVHVRGFTRHESSKTEHPGTYLGVVEKLDHLKDL 276

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            GINC+ELMPC E+NELEYFSYNSVLG+YK+NFWGYST+NYFSPMI YSSAGIRNCGHDAI
Sbjct: 277  GINCLELMPCHEFNELEYFSYNSVLGDYKVNFWGYSTINYFSPMISYSSAGIRNCGHDAI 336

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE K LVREAHKRGIEV+MDVVFNHTAEGN+ GP+LSFRG+DN+VYYMLAPKGEFYNYSG
Sbjct: 337  NEFKLLVREAHKRGIEVVMDVVFNHTAEGNDKGPILSFRGVDNSVYYMLAPKGEFYNYSG 396

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV RQFI+DCLRYWVTE+H+DGFRFDLASIMTR SSLWD+VNV+G PIEGD
Sbjct: 397  CGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDSVNVYGIPIEGD 456

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL SPPLID++SNDPILRGVKLIAEAWDTGGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 457  LLTTGTPLSSPPLIDLISNDPILRGVKLIAEAWDTGGLYQVGIFPHWGIWSEWNGKYRD 515



 Score =  416 bits (1070), Expect(3) = 0.0
 Identities = 191/249 (76%), Positives = 213/249 (85%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQ G RKPWNSINF+CAHDGF+L DLV+YN+KHNLA            NSWNCGQ
Sbjct: 537  GSPNLYQGGGRKPWNSINFVCAHDGFSLADLVSYNQKHNLANGEDNNDGETHNNSWNCGQ 596

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEF    VKKLR+RQMRNFFLCLMVSQGVPM  MGDEYGHTKGGNNNTYCHDN INYFR
Sbjct: 597  EGEFANILVKKLRRRQMRNFFLCLMVSQGVPMISMGDEYGHTKGGNNNTYCHDNDINYFR 656

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+ SDF+RFCCL+TKFRHECESLGL+DFPT++RLQWHGHAP  P+WS+ SRFVAF
Sbjct: 657  WDKKEESKSDFFRFCCLLTKFRHECESLGLSDFPTADRLQWHGHAPGLPDWSDKSRFVAF 716

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            +L+DSVKG+IYVAFN SHLPV+++LPER GYRW PLVDTSK  PFDFLS D+  K++AIK
Sbjct: 717  TLIDSVKGKIYVAFNASHLPVIISLPERPGYRWEPLVDTSKPVPFDFLSSDLPAKEIAIK 776

Query: 508  QYAQFLDGN 482
            QYA FLD N
Sbjct: 777  QYAPFLDAN 785



 Score = 70.1 bits (170), Expect(3) = 0.0
 Identities = 34/62 (54%), Positives = 45/62 (72%)
 Frame = -1

Query: 2521 AEMVVETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDL 2342
            AE+    +EKP    S  F+V  G P P+GAT+++GGVNF++FS  AVS +LCLI+LSDL
Sbjct: 73   AELETAVIEKPP---SQRFQVSKGYPTPFGATLRDGGVNFSIFSSNAVSASLCLITLSDL 129

Query: 2341 QE 2336
            QE
Sbjct: 130  QE 131


>gb|KDO61988.1| hypothetical protein CISIN_1g003653mg [Citrus sinensis]
          Length = 805

 Score =  674 bits (1740), Expect(3) = 0.0
 Identities = 303/359 (84%), Positives = 335/359 (93%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKDMLYGYKFDGKFSP EGHY++ ++IV+DPYAKAVISR ++G  GPD NCWPQMA +
Sbjct: 157  GDFKDMLYGYKFDGKFSPQEGHYFDPTKIVLDPYAKAVISRAQFGVLGPDENCWPQMACL 216

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VP+ + EFDWEGDLPL++ Q+DLIIYE+HVRGFTRHESS T  PGTYLGVVEKLDHLK+L
Sbjct: 217  VPTPEDEFDWEGDLPLKYPQRDLIIYEVHVRGFTRHESSKTEHPGTYLGVVEKLDHLKDL 276

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            GINC+ELMPC E+NELEYFSYNSVLG+YK+NFWGYST+NYFSPMI YSSAGIRNCGHDAI
Sbjct: 277  GINCLELMPCHEFNELEYFSYNSVLGDYKVNFWGYSTINYFSPMISYSSAGIRNCGHDAI 336

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE K LVREAHKRGIEV+MDVVFNHT EGN+ GP+LSFRG+DN+VYYMLAPKGEFYNYSG
Sbjct: 337  NEFKLLVREAHKRGIEVVMDVVFNHTVEGNDKGPILSFRGVDNSVYYMLAPKGEFYNYSG 396

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV RQFI+DCLRYWVTE+H+DGFRFDLASIMTR SSLWD+VNV+G PIEGD
Sbjct: 397  CGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDSVNVYGIPIEGD 456

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL SPPLID++SNDPILRGVKLIAEAWDTGGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 457  LLTTGTPLRSPPLIDLISNDPILRGVKLIAEAWDTGGLYQVGIFPHWGIWSEWNGKYRD 515



 Score =  416 bits (1069), Expect(3) = 0.0
 Identities = 191/249 (76%), Positives = 213/249 (85%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQ G RKPWNSINF+CAHDGF+L DLV+YN+KHNLA            NSWNCGQ
Sbjct: 537  GSPNLYQGGGRKPWNSINFVCAHDGFSLADLVSYNQKHNLANGEDNNDGETHNNSWNCGQ 596

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEF    VKKLR+RQMRNFFLCLMVSQGVPM  MGDEYGHTKGGNNNTYCHDN INYFR
Sbjct: 597  EGEFANILVKKLRRRQMRNFFLCLMVSQGVPMISMGDEYGHTKGGNNNTYCHDNDINYFR 656

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+ SDF+RFCCL+TKFRHECESLGL+DFPT++RLQWHGHAP  P+WS+ SRFVAF
Sbjct: 657  WDKKEESKSDFFRFCCLLTKFRHECESLGLSDFPTADRLQWHGHAPGLPDWSDKSRFVAF 716

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            +L+DSVKGEIYVAFN SHLPV+++LP+R GYRW PLVDTSK  PFDFLS D+  K++AIK
Sbjct: 717  TLIDSVKGEIYVAFNASHLPVIISLPKRPGYRWEPLVDTSKPEPFDFLSSDLPAKEIAIK 776

Query: 508  QYAQFLDGN 482
            QYA FLD N
Sbjct: 777  QYAPFLDAN 785



 Score = 70.9 bits (172), Expect(3) = 0.0
 Identities = 34/62 (54%), Positives = 45/62 (72%)
 Frame = -1

Query: 2521 AEMVVETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDL 2342
            AE+    ++KP    S  F+V  G P P+GAT+++GGVNF++FS  AVS TLCLI+LSDL
Sbjct: 73   AELETAVIKKPQ---SQRFQVSKGYPTPFGATLRDGGVNFSIFSSNAVSATLCLITLSDL 129

Query: 2341 QE 2336
            QE
Sbjct: 130  QE 131


>ref|XP_010048547.1| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Eucalyptus
            grandis] gi|629116149|gb|KCW80824.1| hypothetical protein
            EUGRSUZ_C02189 [Eucalyptus grandis]
          Length = 794

 Score =  674 bits (1739), Expect(3) = 0.0
 Identities = 304/359 (84%), Positives = 332/359 (92%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDF D+LYGYKFDGK+SP+EGH Y++S I++DPYAKAVISRGEYG  GPDG+CWPQMA M
Sbjct: 146  GDFTDVLYGYKFDGKYSPEEGHRYDASHILLDPYAKAVISRGEYGVLGPDGDCWPQMACM 205

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VPS   EFDWEGDLPL++H KDLIIYEMHVRGFTRH+SS T  PGTY GVVEKLDHLKEL
Sbjct: 206  VPSDGDEFDWEGDLPLKYHPKDLIIYEMHVRGFTRHDSSKTDFPGTYRGVVEKLDHLKEL 265

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            GINCIELMPC E+NELEYFSYN+VLG+YKMNFWGYST+NYFSPM+RYSSAG+ +CG DAI
Sbjct: 266  GINCIELMPCHEFNELEYFSYNTVLGDYKMNFWGYSTINYFSPMVRYSSAGLSSCGQDAI 325

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE K L++EAHKRGIEV++DVVFNHTAEGNENGPVLSFRGIDN+VYYMLAPKGEFYNYSG
Sbjct: 326  NEFKTLIKEAHKRGIEVLLDVVFNHTAEGNENGPVLSFRGIDNSVYYMLAPKGEFYNYSG 385

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNT NCNHP  RQFI+DCLRYW TE+H+DGFRFDLASI+TRSSSLWDA NVFG PIEGD
Sbjct: 386  CGNTLNCNHPAVRQFIVDCLRYWATEMHVDGFRFDLASILTRSSSLWDAANVFGKPIEGD 445

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LLTTGTPL SPPLIDM+SNDPILRGVKLIAEAWDTGGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 446  LLTTGTPLSSPPLIDMISNDPILRGVKLIAEAWDTGGLYQVGTFPHWGIWSEWNGKYRD 504



 Score =  429 bits (1104), Expect(3) = 0.0
 Identities = 193/249 (77%), Positives = 217/249 (87%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQEG R+PWNS+NF+CAHDGFTL DLV+YN+KHNL             NSWNCGQ
Sbjct: 526  GSPNLYQEGGRRPWNSVNFVCAHDGFTLADLVSYNDKHNLVNGEDNNDGESHNNSWNCGQ 585

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEF G  VK+LRKRQMRNFF+CLMVSQG+PM YMGDEYGHTKGGNNN+YCHDNYINYFR
Sbjct: 586  EGEFAGISVKRLRKRQMRNFFVCLMVSQGIPMLYMGDEYGHTKGGNNNSYCHDNYINYFR 645

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+SSDFYRFC L+TKFRHECESLGL+DFPT+ERLQWHGH P +P+WSE SRFVA+
Sbjct: 646  WDKKEESSSDFYRFCSLMTKFRHECESLGLSDFPTAERLQWHGHLPGKPDWSENSRFVAY 705

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            ++VDSV+GEIY+AFN SHLPV V LPER GYRW PLVDTSK APFDFLS D+ E+D+A+K
Sbjct: 706  TMVDSVRGEIYIAFNASHLPVTVVLPERPGYRWEPLVDTSKPAPFDFLSSDLPERDVAVK 765

Query: 508  QYAQFLDGN 482
            QYAQFLD N
Sbjct: 766  QYAQFLDSN 774



 Score = 56.2 bits (134), Expect(3) = 0.0
 Identities = 31/61 (50%), Positives = 38/61 (62%)
 Frame = -1

Query: 2518 EMVVETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQ 2339
            E+    VE+P     S+F+V    P P GAT   GGVNF+V+S  A S TLCLISL+DL 
Sbjct: 63   EVETAMVERPRP---SSFQVSRVRPTPLGATACVGGVNFSVYSSSAASATLCLISLADLN 119

Query: 2338 E 2336
            E
Sbjct: 120  E 120


>ref|XP_003630623.2| isoamylase-type starch debranching enzyme [Medicago truncatula]
            gi|657376100|gb|AET05099.2| isoamylase-type starch
            debranching enzyme [Medicago truncatula]
          Length = 788

 Score =  672 bits (1733), Expect(3) = 0.0
 Identities = 300/359 (83%), Positives = 333/359 (92%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKDMLY YKFDGKFSP +GHYY+SSRI++DPYAKAVISRGE+G  GPDGNCWPQMAGM
Sbjct: 141  GDFKDMLYAYKFDGKFSPQQGHYYDSSRILIDPYAKAVISRGEFGEVGPDGNCWPQMAGM 200

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VP  + EFDWEGDLPL++ QKDLIIYEMHVRGFT+HESS    PGTYLGVVEKLDHLKEL
Sbjct: 201  VPFDNEEFDWEGDLPLKYPQKDLIIYEMHVRGFTKHESSKAEFPGTYLGVVEKLDHLKEL 260

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            G+NCIELMPC E+NELEY+SYN++ G+Y++NFWGYST+NYFSPMIRYSSAGI+NCG D I
Sbjct: 261  GVNCIELMPCHEFNELEYYSYNAIQGDYRVNFWGYSTINYFSPMIRYSSAGIQNCGRDGI 320

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE+K L++EAHKRGIEVIMDVVFNHTAEGNE GP++SFRG+DN+VYYM+APKGEFYNYSG
Sbjct: 321  NEMKLLIKEAHKRGIEVIMDVVFNHTAEGNEKGPIISFRGVDNSVYYMVAPKGEFYNYSG 380

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV R+FI+DCLRYWVTE+H+DGFRFDLASIMTRSSSLW+ VNVFG PIEGD
Sbjct: 381  CGNTFNCNHPVVRKFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWNGVNVFGAPIEGD 440

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
             LTTGTPL SPPLIDM+S DPILRGVKLIAEAWD GGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 441  FLTTGTPLSSPPLIDMISIDPILRGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYRD 499



 Score =  417 bits (1071), Expect(3) = 0.0
 Identities = 189/248 (76%), Positives = 211/248 (85%)
 Frame = -1

Query: 1225 GSPNLYQEGRKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQE 1046
            GSPNLYQ GRKPWNSIN +CAHDGFTL DLVTYN KHNL             +SWNCG+E
Sbjct: 521  GSPNLYQGGRKPWNSINLVCAHDGFTLADLVTYNNKHNLPNGEDNNDGENHNSSWNCGEE 580

Query: 1045 GEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFRW 866
            GEFV   VKK+RKRQMRNFFL LMVSQGVPM +MGDEYGHTKGGNNNTYCHDNY+NYFRW
Sbjct: 581  GEFVSASVKKMRKRQMRNFFLSLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYLNYFRW 640

Query: 865  DKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAFS 686
            DKKEE+SSDF+RFC L+TKFR ECESLGL+DFPTSERLQWHGH P  P+WSE+SRFVAF+
Sbjct: 641  DKKEESSSDFFRFCRLLTKFRQECESLGLDDFPTSERLQWHGHFPVTPDWSESSRFVAFT 700

Query: 685  LVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIKQ 506
            L+D VKGE+YVAFNTSHLP  +TLPER GYRW PLVDTSK AP+DFL+ D+  +D+AI+Q
Sbjct: 701  LMDLVKGEVYVAFNTSHLPFTITLPERPGYRWEPLVDTSKKAPYDFLTPDLPGRDIAIQQ 760

Query: 505  YAQFLDGN 482
            YAQFLD N
Sbjct: 761  YAQFLDAN 768



 Score = 45.4 bits (106), Expect(3) = 0.0
 Identities = 22/57 (38%), Positives = 33/57 (57%)
 Frame = -1

Query: 2509 VETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQ 2339
            V+T     ++     ++  G P PYGAT +  GVNFA+ S  ++S TLC  +LSD +
Sbjct: 58   VDTETAVIEIEKRQLQLSKGHPSPYGATPQEDGVNFAINSLNSLSATLCFFTLSDFK 114


>ref|XP_002529900.1| isoamylase, putative [Ricinus communis] gi|223530627|gb|EEF32503.1|
            isoamylase, putative [Ricinus communis]
          Length = 795

 Score =  671 bits (1730), Expect(3) = 0.0
 Identities = 304/360 (84%), Positives = 330/360 (91%), Gaps = 1/360 (0%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKD LYGY+FDG  SP +GHY++SS+IV+DPYAKAVISRGE+G  GP  NCWPQMAGM
Sbjct: 148  GDFKDTLYGYRFDGVLSPHDGHYFDSSQIVLDPYAKAVISRGEFGVLGPGDNCWPQMAGM 207

Query: 2136 VPS-SDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKE 1960
            +PS S  EFDWEGDLPL++ QKDL+IYEMHVRGFTRHESS T  PGT+L  VEKLDHLKE
Sbjct: 208  IPSASQAEFDWEGDLPLKYPQKDLVIYEMHVRGFTRHESSRTEFPGTFLATVEKLDHLKE 267

Query: 1959 LGINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDA 1780
            LG+NCIELMPC E+NELEY+SYNSVLG+YKMNFWGYST+NYFSPM RYSSAG RNCG DA
Sbjct: 268  LGVNCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYSTINYFSPMTRYSSAGTRNCGRDA 327

Query: 1779 INEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYS 1600
            INE KFLVREAHKRGIEVIMDVVFNHTAEGN+ GP+LSFRG+DN+VYYMLAPKGEFYNYS
Sbjct: 328  INEFKFLVREAHKRGIEVIMDVVFNHTAEGNQKGPILSFRGVDNSVYYMLAPKGEFYNYS 387

Query: 1599 GCGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEG 1420
            GCGNTFNCNHP+ RQFILDCLRYWV E+H+DGFRFDLASIMTR SSLWDAVNVFGNPIEG
Sbjct: 388  GCGNTFNCNHPIVRQFILDCLRYWVLEMHVDGFRFDLASIMTRGSSLWDAVNVFGNPIEG 447

Query: 1419 DLLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            DLLTTGTPL SPPLIDM+SNDPIL GVKL+AEAWDTGGLYQVG FPHW IWSEWNGKYRD
Sbjct: 448  DLLTTGTPLSSPPLIDMISNDPILHGVKLVAEAWDTGGLYQVGSFPHWQIWSEWNGKYRD 507



 Score =  411 bits (1057), Expect(3) = 0.0
 Identities = 191/241 (79%), Positives = 206/241 (85%), Gaps = 1/241 (0%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQEG RKPWNSINFICAHDGFTL DLV+YN K+NLA            NSWNCGQ
Sbjct: 529  GSPNLYQEGGRKPWNSINFICAHDGFTLADLVSYNNKNNLANGEDNNDGESHNNSWNCGQ 588

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFR 869
            EGEF    VKKLRKRQMRNFF+CLMVSQGVPM YMGDEYGHTKGGNNNTYCHDNYINYFR
Sbjct: 589  EGEFASILVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFR 648

Query: 868  WDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            WDKKEE+SSDFYRFC L+TKFRHECESLGLNDFPT+ERLQWHGH+P  P+WSETSRFVAF
Sbjct: 649  WDKKEESSSDFYRFCRLMTKFRHECESLGLNDFPTAERLQWHGHSPGMPDWSETSRFVAF 708

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            +L DSVKGEIYVAFN +HLPV + LPER GYRW PLVDT K APFDF S D+ ++D+A K
Sbjct: 709  TLNDSVKGEIYVAFNANHLPVTIGLPERPGYRWQPLVDTGKPAPFDFFSSDIPDRDIAYK 768

Query: 508  Q 506
            Q
Sbjct: 769  Q 769



 Score = 60.8 bits (146), Expect(3) = 0.0
 Identities = 29/58 (50%), Positives = 39/58 (67%)
 Frame = -1

Query: 2509 VETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQE 2336
            VET    +K     ++V  G P P+GAT+ +GGVNFA++S  AVS +LCLISL DL +
Sbjct: 65   VETAVVVDKPKFRRYQVSEGHPAPFGATIADGGVNFAIYSSDAVSASLCLISLDDLTQ 122


>ref|XP_010106957.1| Isoamylase 1 [Morus notabilis] gi|587925578|gb|EXC12839.1| Isoamylase
            1 [Morus notabilis]
          Length = 805

 Score =  668 bits (1724), Expect(3) = 0.0
 Identities = 301/358 (84%), Positives = 330/358 (92%)
 Frame = -2

Query: 2313 DFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGMV 2134
            DFKDMLYGYKFDGKFSP+EGHYY+ SRIV+DPY KAVI RGEYG  GPDGNCWPQMA  V
Sbjct: 144  DFKDMLYGYKFDGKFSPEEGHYYDPSRIVLDPYGKAVICRGEYGTLGPDGNCWPQMACAV 203

Query: 2133 PSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKELG 1954
            P  D EFDWEGDLPL++ Q+DLI+YEMHVRGFTRHESS T  PGTYLGVVEKL HL+ELG
Sbjct: 204  PFFDNEFDWEGDLPLKYPQEDLIVYEMHVRGFTRHESSGTEFPGTYLGVVEKLHHLQELG 263

Query: 1953 INCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAIN 1774
            +NCIELMPC E+NELEY+SYNS +G+YK+NFWGYSTVNYFSPM+RYSSAG  NCGHDA+N
Sbjct: 264  VNCIELMPCHEFNELEYYSYNSAIGDYKVNFWGYSTVNYFSPMLRYSSAGTHNCGHDAVN 323

Query: 1773 EVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSGC 1594
            E+K L+REAHKRGIEVIMDVVFNHTAEGNENGP+LSFRG DN+VYYMLAPKGEFYNYSGC
Sbjct: 324  ELKLLIREAHKRGIEVIMDVVFNHTAEGNENGPILSFRGADNSVYYMLAPKGEFYNYSGC 383

Query: 1593 GNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGDL 1414
            GNTFNCNHPV RQFI+DCLRYWVTE+H+DGFRFDLASI+TR SSL+DAVN++G+PIEG L
Sbjct: 384  GNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASILTRGSSLYDAVNIYGSPIEGAL 443

Query: 1413 LTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LTTGTPL SPPLIDM+SNDPIL GVKLIAEAWD GGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 444  LTTGTPLSSPPLIDMMSNDPILCGVKLIAEAWDAGGLYQVGSFPHWGIWSEWNGKYRD 501



 Score =  416 bits (1070), Expect(3) = 0.0
 Identities = 194/261 (74%), Positives = 213/261 (81%), Gaps = 13/261 (4%)
 Frame = -1

Query: 1225 GSPNLYQEG-RKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQ 1049
            GSPNLYQEG RKPW+SINF+CAHDGFTL DLV YN KHNLA            NSWNCGQ
Sbjct: 523  GSPNLYQEGGRKPWHSINFVCAHDGFTLADLVAYNNKHNLANGEDNKDGENHNNSWNCGQ 582

Query: 1048 EGEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNY----- 884
            EG+FV   VKKLRKRQM+NFFLCLMVSQGVPM YMGDEYGHTKGGNNNTYCHDNY     
Sbjct: 583  EGKFVSISVKKLRKRQMKNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYTQLSI 642

Query: 883  -------INYFRWDKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCE 725
                   INYFRWDKKE++SSDF+RFCCL+TKFR ECESLGL DFPT+ERLQWHGHAP  
Sbjct: 643  LTVTGLQINYFRWDKKEDSSSDFFRFCCLVTKFRRECESLGLKDFPTAERLQWHGHAPET 702

Query: 724  PNWSETSRFVAFSLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFL 545
            P+WS+TSRFVAFSL DS+KGEIYVAFN SHLPV ++LPER GYRW PLVDT K AP+DFL
Sbjct: 703  PDWSKTSRFVAFSLKDSIKGEIYVAFNASHLPVTISLPERPGYRWEPLVDTGKPAPYDFL 762

Query: 544  SDDVSEKDLAIKQYAQFLDGN 482
            S DV E+++ +KQYA FLD N
Sbjct: 763  SPDVPEREIVVKQYAHFLDAN 783



 Score = 67.0 bits (162), Expect(3) = 0.0
 Identities = 30/46 (65%), Positives = 39/46 (84%)
 Frame = -1

Query: 2473 SNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSDLQE 2336
            S F+V  GSP+P GAT+++GGVNFAVF+G AV  TLCL+SLSDL++
Sbjct: 72   SRFQVSRGSPVPLGATLRDGGVNFAVFAGNAVKATLCLMSLSDLEQ 117


>ref|XP_004503543.1| PREDICTED: isoamylase 1, chloroplastic isoform X2 [Cicer arietinum]
          Length = 792

 Score =  668 bits (1723), Expect(3) = 0.0
 Identities = 297/359 (82%), Positives = 332/359 (92%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKDMLYGYKFDGKFSP +GHYY+SSR+++DPYAKAVIS+GE+G  GPDGNCWPQMAGM
Sbjct: 145  GDFKDMLYGYKFDGKFSPQQGHYYDSSRLLLDPYAKAVISKGEFGVVGPDGNCWPQMAGM 204

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VP    EFDWEGDLPL++ QKDLIIYE+HVRGFT+HESS T  PGTYLGVVEKLDHLK+L
Sbjct: 205  VPFDHEEFDWEGDLPLKYPQKDLIIYELHVRGFTKHESSNTKFPGTYLGVVEKLDHLKDL 264

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            G+NCIELMPC E+NELEY+SYN   G+Y++NFWGYST+NYFSPMIRYSSA I+NCG D I
Sbjct: 265  GVNCIELMPCHEFNELEYYSYNPGQGDYRVNFWGYSTINYFSPMIRYSSADIQNCGPDGI 324

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE+KFL++EAHKRGIEV+MDVVFNHTAEGNE GP++SFRG+DN+VYYM+APKGEFYNYSG
Sbjct: 325  NEIKFLIKEAHKRGIEVVMDVVFNHTAEGNEKGPIISFRGVDNSVYYMVAPKGEFYNYSG 384

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV R+FI+DCLRYWVTE+H+DGFRFDLASIMTR SSLW+ VNVFG PIEGD
Sbjct: 385  CGNTFNCNHPVVRKFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWNGVNVFGAPIEGD 444

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LL TGTPL SPPLIDM+SNDPILRGVKLIAEAWD GGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 445  LLATGTPLSSPPLIDMISNDPILRGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYRD 503



 Score =  420 bits (1080), Expect(3) = 0.0
 Identities = 191/248 (77%), Positives = 212/248 (85%)
 Frame = -1

Query: 1225 GSPNLYQEGRKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQE 1046
            GSPNLYQ GRKPW+SIN +CAHDGFTL DLVTYN KHNL             NSWNCG+E
Sbjct: 525  GSPNLYQRGRKPWHSINLVCAHDGFTLADLVTYNNKHNLPNGEDNNDGENHNNSWNCGEE 584

Query: 1045 GEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFRW 866
            GEFV   VKKLRKRQMRNFFL LMVSQGVPM YMGDEYGHTKGGNNNTYCHDNY+NYFRW
Sbjct: 585  GEFVSASVKKLRKRQMRNFFLSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYLNYFRW 644

Query: 865  DKKEETSSDFYRFCCLITKFRHECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAFS 686
            DKKEE+SSDFYRFC L+TKFR +CESLGL+DFPTS+RLQWHGH P  P+WSETSRFVAF+
Sbjct: 645  DKKEESSSDFYRFCRLLTKFRQQCESLGLDDFPTSDRLQWHGHFPATPDWSETSRFVAFT 704

Query: 685  LVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIKQ 506
            L+DSVKGEIY+AFNTSHLP  +TLPER GYRW PLVDTSK+AP+DFL+ D+  +D+AI+Q
Sbjct: 705  LMDSVKGEIYIAFNTSHLPFTITLPERPGYRWEPLVDTSKSAPYDFLTPDLPGRDIAIQQ 764

Query: 505  YAQFLDGN 482
            YA FLD N
Sbjct: 765  YAHFLDAN 772



 Score = 55.8 bits (133), Expect(3) = 0.0
 Identities = 28/58 (48%), Positives = 37/58 (63%)
 Frame = -1

Query: 2518 EMVVETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSD 2345
            E+ V  +EKP       FEV  G P P+GATV+  GVNFA++S  ++S TLCL +L D
Sbjct: 65   EIAVVEIEKPQ------FEVSKGYPSPFGATVREDGVNFAIYSLNSLSATLCLFTLCD 116


>ref|XP_004503542.1| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Cicer arietinum]
          Length = 793

 Score =  668 bits (1723), Expect(3) = 0.0
 Identities = 297/359 (82%), Positives = 332/359 (92%)
 Frame = -2

Query: 2316 GDFKDMLYGYKFDGKFSPDEGHYYNSSRIVVDPYAKAVISRGEYGAFGPDGNCWPQMAGM 2137
            GDFKDMLYGYKFDGKFSP +GHYY+SSR+++DPYAKAVIS+GE+G  GPDGNCWPQMAGM
Sbjct: 145  GDFKDMLYGYKFDGKFSPQQGHYYDSSRLLLDPYAKAVISKGEFGVVGPDGNCWPQMAGM 204

Query: 2136 VPSSDFEFDWEGDLPLRHHQKDLIIYEMHVRGFTRHESSMTSSPGTYLGVVEKLDHLKEL 1957
            VP    EFDWEGDLPL++ QKDLIIYE+HVRGFT+HESS T  PGTYLGVVEKLDHLK+L
Sbjct: 205  VPFDHEEFDWEGDLPLKYPQKDLIIYELHVRGFTKHESSNTKFPGTYLGVVEKLDHLKDL 264

Query: 1956 GINCIELMPCQEYNELEYFSYNSVLGNYKMNFWGYSTVNYFSPMIRYSSAGIRNCGHDAI 1777
            G+NCIELMPC E+NELEY+SYN   G+Y++NFWGYST+NYFSPMIRYSSA I+NCG D I
Sbjct: 265  GVNCIELMPCHEFNELEYYSYNPGQGDYRVNFWGYSTINYFSPMIRYSSADIQNCGPDGI 324

Query: 1776 NEVKFLVREAHKRGIEVIMDVVFNHTAEGNENGPVLSFRGIDNNVYYMLAPKGEFYNYSG 1597
            NE+KFL++EAHKRGIEV+MDVVFNHTAEGNE GP++SFRG+DN+VYYM+APKGEFYNYSG
Sbjct: 325  NEIKFLIKEAHKRGIEVVMDVVFNHTAEGNEKGPIISFRGVDNSVYYMVAPKGEFYNYSG 384

Query: 1596 CGNTFNCNHPVARQFILDCLRYWVTEVHIDGFRFDLASIMTRSSSLWDAVNVFGNPIEGD 1417
            CGNTFNCNHPV R+FI+DCLRYWVTE+H+DGFRFDLASIMTR SSLW+ VNVFG PIEGD
Sbjct: 385  CGNTFNCNHPVVRKFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWNGVNVFGAPIEGD 444

Query: 1416 LLTTGTPLDSPPLIDMLSNDPILRGVKLIAEAWDTGGLYQVGKFPHWGIWSEWNGKYRD 1240
            LL TGTPL SPPLIDM+SNDPILRGVKLIAEAWD GGLYQVG FPHWGIWSEWNGKYRD
Sbjct: 445  LLATGTPLSSPPLIDMISNDPILRGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYRD 503



 Score =  419 bits (1076), Expect(3) = 0.0
 Identities = 192/249 (77%), Positives = 213/249 (85%), Gaps = 1/249 (0%)
 Frame = -1

Query: 1225 GSPNLYQEGRKPWNSINFICAHDGFTLGDLVTYNEKHNLAXXXXXXXXXXXXNSWNCGQE 1046
            GSPNLYQ GRKPW+SIN +CAHDGFTL DLVTYN KHNL             NSWNCG+E
Sbjct: 525  GSPNLYQRGRKPWHSINLVCAHDGFTLADLVTYNNKHNLPNGEDNNDGENHNNSWNCGEE 584

Query: 1045 GEFVGFPVKKLRKRQMRNFFLCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDNYINYFRW 866
            GEFV   VKKLRKRQMRNFFL LMVSQGVPM YMGDEYGHTKGGNNNTYCHDNY+NYFRW
Sbjct: 585  GEFVSASVKKLRKRQMRNFFLSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYLNYFRW 644

Query: 865  DKKEETSSDFYRFCCLITKFRH-ECESLGLNDFPTSERLQWHGHAPCEPNWSETSRFVAF 689
            DKKEE+SSDFYRFC L+TKFRH +CESLGL+DFPTS+RLQWHGH P  P+WSETSRFVAF
Sbjct: 645  DKKEESSSDFYRFCRLLTKFRHRQCESLGLDDFPTSDRLQWHGHFPATPDWSETSRFVAF 704

Query: 688  SLVDSVKGEIYVAFNTSHLPVMVTLPERQGYRWTPLVDTSKAAPFDFLSDDVSEKDLAIK 509
            +L+DSVKGEIY+AFNTSHLP  +TLPER GYRW PLVDTSK+AP+DFL+ D+  +D+AI+
Sbjct: 705  TLMDSVKGEIYIAFNTSHLPFTITLPERPGYRWEPLVDTSKSAPYDFLTPDLPGRDIAIQ 764

Query: 508  QYAQFLDGN 482
            QYA FLD N
Sbjct: 765  QYAHFLDAN 773



 Score = 55.8 bits (133), Expect(3) = 0.0
 Identities = 28/58 (48%), Positives = 37/58 (63%)
 Frame = -1

Query: 2518 EMVVETVEKPNKLLSSNFEVFSGSPMPYGATVKNGGVNFAVFSGGAVSVTLCLISLSD 2345
            E+ V  +EKP       FEV  G P P+GATV+  GVNFA++S  ++S TLCL +L D
Sbjct: 65   EIAVVEIEKPQ------FEVSKGYPSPFGATVREDGVNFAIYSLNSLSATLCLFTLCD 116