BLASTX nr result
ID: Papaver29_contig00039536
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver29_contig00039536
(495 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002281960.1| PREDICTED: DNA-binding protein RHL1 [Vitis v... 79 1e-12
ref|XP_011072071.1| PREDICTED: DNA-binding protein RHL1 isoform ... 74 6e-11
ref|XP_011072069.1| PREDICTED: DNA-binding protein RHL1 isoform ... 73 7e-11
ref|XP_008803062.1| PREDICTED: DNA-binding protein RHL1 [Phoenix... 70 6e-10
ref|XP_013452847.1| DNA-binding protein RHL1, putative [Medicago... 69 2e-09
ref|XP_013452846.1| DNA-binding protein RHL1, putative [Medicago... 69 2e-09
ref|XP_013452845.1| DNA-binding protein RHL1, putative [Medicago... 69 2e-09
ref|XP_003620153.2| DNA-binding protein RHL1, putative [Medicago... 69 2e-09
ref|XP_010935551.1| PREDICTED: DNA-binding protein RHL1-like [El... 67 4e-09
gb|KMT03733.1| hypothetical protein BVRB_8g188820 isoform A [Bet... 67 7e-09
ref|XP_010687257.1| PREDICTED: DNA-binding protein RHL1 isoform ... 67 7e-09
ref|XP_010687256.1| PREDICTED: DNA-binding protein RHL1 isoform ... 67 7e-09
ref|XP_010687255.1| PREDICTED: DNA-binding protein RHL1 isoform ... 67 7e-09
gb|KNA18952.1| hypothetical protein SOVF_065990 [Spinacia oleracea] 66 9e-09
gb|AGJ83755.1| DNA-binding protein RHL1-like protein, partial [C... 66 9e-09
ref|XP_009398296.1| PREDICTED: DNA-binding protein RHL1 [Musa ac... 64 3e-08
ref|XP_012476900.1| PREDICTED: DNA-binding protein RHL1 isoform ... 64 6e-08
ref|XP_012476896.1| PREDICTED: DNA-binding protein RHL1 isoform ... 64 6e-08
ref|XP_012476895.1| PREDICTED: DNA-binding protein RHL1 isoform ... 64 6e-08
gb|KJB26814.1| hypothetical protein B456_004G262200 [Gossypium r... 64 6e-08
>ref|XP_002281960.1| PREDICTED: DNA-binding protein RHL1 [Vitis vinifera]
gi|731421942|ref|XP_010661929.1| PREDICTED: DNA-binding
protein RHL1 [Vitis vinifera]
Length = 449
Score = 79.0 bits (193), Expect = 1e-12
Identities = 53/129 (41%), Positives = 64/129 (49%), Gaps = 1/129 (0%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGAGPEEKFGGIKPREEKRKPQSPTDSLEXXXXXXXXXC 183
RLEFPKEL E + V++DFKGGAG + K G KP + +PQSP LE
Sbjct: 163 RLEFPKELSEGQSVEYDFKGGAGMASDSKQGVNKPEMKYVEPQSPKPELEDDLSGEDSLK 222
Query: 184 PSPGITLKDGMEVTPVRQSARTSGRRFNFAEXXXXXXXXXXXXTDVDLSEIKGKKVIEE- 360
+T KD +EVTPVR S RT+G+ FNFAE D E G E
Sbjct: 223 DVVEMTPKD-VEVTPVRHSQRTAGKTFNFAEASSGDDSVENDGNISDGQENSGSATPESG 281
Query: 361 TEDAKTLKG 387
EDA+ G
Sbjct: 282 NEDAEARTG 290
>ref|XP_011072071.1| PREDICTED: DNA-binding protein RHL1 isoform X3 [Sesamum indicum]
Length = 368
Score = 73.6 bits (179), Expect = 6e-11
Identities = 57/157 (36%), Positives = 79/157 (50%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGAGPEEKFGGIKPREEKRKPQSPTDSLEXXXXXXXXXC 183
RL+FPKEL EK V++DFKGGAGA + K G K + + +SP L+
Sbjct: 161 RLQFPKELNLEKQVEYDFKGGAGATQDSKLGSGKFATKCLEQESPEVCLD------NNFA 214
Query: 184 PSPGITLKDGMEVTPVRQSARTSGRRFNFAEXXXXXXXXXXXXTDVDLSEIKGKKVIEET 363
SP K+ EVTP RQSART+GR FNFAE + E + +KV
Sbjct: 215 DSPN-DYKELAEVTPTRQSARTAGRTFNFAEASSGDDFVGDGD---ETPEEENEKV---- 266
Query: 364 EDAKTLKGIPNLKKTAKSRLVLPDSSNDIPAKDNQSS 474
D++T K + + SRLV + N+ A+++ S
Sbjct: 267 -DSETRKTYTSTETKISSRLVF-EIDNEANAREDPGS 301
>ref|XP_011072069.1| PREDICTED: DNA-binding protein RHL1 isoform X1 [Sesamum indicum]
gi|747051948|ref|XP_011072070.1| PREDICTED: DNA-binding
protein RHL1 isoform X2 [Sesamum indicum]
Length = 370
Score = 73.2 bits (178), Expect = 7e-11
Identities = 60/157 (38%), Positives = 79/157 (50%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGAGPEEKFGGIKPREEKRKPQSPTDSLEXXXXXXXXXC 183
RL+FPKEL EK V++DFKGGAGA + K G K + + +SP L+
Sbjct: 161 RLQFPKELNLEKQVEYDFKGGAGATQDSKLGSGKFATKCLEQESPEVCLD------NNFA 214
Query: 184 PSPGITLKDGMEVTPVRQSARTSGRRFNFAEXXXXXXXXXXXXTDVDLSEIKGKKVIEET 363
SP K+ EVTP RQSART+GR FNFAE + E + +KV ET
Sbjct: 215 DSPN-DYKELAEVTPTRQSARTAGRTFNFAEASSGDDFVGDGD---ETPEEENEKVDSET 270
Query: 364 EDAKTLKGIPNLKKTAKSRLVLPDSSNDIPAKDNQSS 474
KT L K + SRLV + N+ A+++ S
Sbjct: 271 R--KTYTSTDILTKIS-SRLVF-EIDNEANAREDPGS 303
>ref|XP_008803062.1| PREDICTED: DNA-binding protein RHL1 [Phoenix dactylifera]
Length = 432
Score = 70.1 bits (170), Expect = 6e-10
Identities = 42/93 (45%), Positives = 54/93 (58%), Gaps = 1/93 (1%)
Frame = +1
Query: 1 VRLEFPKELKEEKHV-DHDFKGGAGAGPEEKFGGIKPREEKRKPQSPTDSLEXXXXXXXX 177
++LEFPK L E KH D DFKGGAGA EE G KP ++ +P SP +E
Sbjct: 160 LKLEFPKNLSEGKHAADCDFKGGAGATVEELSGANKPGKDYVEPLSPDTDMEGDTPEDSH 219
Query: 178 XCPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
+ K+ E TPVRQSART+G++ N+AE
Sbjct: 220 FKTEE--STKNLAEATPVRQSARTAGKQLNYAE 250
>ref|XP_013452847.1| DNA-binding protein RHL1, putative [Medicago truncatula]
gi|657383098|gb|KEH26875.1| DNA-binding protein RHL1,
putative [Medicago truncatula]
Length = 346
Score = 68.6 bits (166), Expect = 2e-09
Identities = 49/127 (38%), Positives = 63/127 (49%), Gaps = 6/127 (4%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGAGPEE------KFGGIKPREEKRKPQSPTDSLEXXXX 165
+LEFPKEL E K +HDFKGGAGAG G K + ++ +P+SP LE
Sbjct: 167 KLEFPKELYEGKQTEHDFKGGAGAGAGAGAASVVNHGVSKTKIKRPEPESPETPLE---- 222
Query: 166 XXXXXCPSPGITLKDGMEVTPVRQSARTSGRRFNFAEXXXXXXXXXXXXTDVDLSEIKGK 345
I LKD E+ PVRQSART + + FAE + DLSE + K
Sbjct: 223 ---EDLSDSEIELKDTTELVPVRQSARTVKKSYKFAE---ISSGDDSGKSSPDLSEHEEK 276
Query: 346 KVIEETE 366
V +T+
Sbjct: 277 AVEVDTD 283
>ref|XP_013452846.1| DNA-binding protein RHL1, putative [Medicago truncatula]
gi|657383097|gb|KEH26874.1| DNA-binding protein RHL1,
putative [Medicago truncatula]
Length = 389
Score = 68.6 bits (166), Expect = 2e-09
Identities = 49/127 (38%), Positives = 63/127 (49%), Gaps = 6/127 (4%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGAGPEE------KFGGIKPREEKRKPQSPTDSLEXXXX 165
+LEFPKEL E K +HDFKGGAGAG G K + ++ +P+SP LE
Sbjct: 167 KLEFPKELYEGKQTEHDFKGGAGAGAGAGAASVVNHGVSKTKIKRPEPESPETPLE---- 222
Query: 166 XXXXXCPSPGITLKDGMEVTPVRQSARTSGRRFNFAEXXXXXXXXXXXXTDVDLSEIKGK 345
I LKD E+ PVRQSART + + FAE + DLSE + K
Sbjct: 223 ---EDLSDSEIELKDTTELVPVRQSARTVKKSYKFAE---ISSGDDSGKSSPDLSEHEEK 276
Query: 346 KVIEETE 366
V +T+
Sbjct: 277 AVEVDTD 283
>ref|XP_013452845.1| DNA-binding protein RHL1, putative [Medicago truncatula]
gi|657383096|gb|KEH26873.1| DNA-binding protein RHL1,
putative [Medicago truncatula]
Length = 390
Score = 68.6 bits (166), Expect = 2e-09
Identities = 49/127 (38%), Positives = 63/127 (49%), Gaps = 6/127 (4%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGAGPEE------KFGGIKPREEKRKPQSPTDSLEXXXX 165
+LEFPKEL E K +HDFKGGAGAG G K + ++ +P+SP LE
Sbjct: 167 KLEFPKELYEGKQTEHDFKGGAGAGAGAGAASVVNHGVSKTKIKRPEPESPETPLE---- 222
Query: 166 XXXXXCPSPGITLKDGMEVTPVRQSARTSGRRFNFAEXXXXXXXXXXXXTDVDLSEIKGK 345
I LKD E+ PVRQSART + + FAE + DLSE + K
Sbjct: 223 ---EDLSDSEIELKDTTELVPVRQSARTVKKSYKFAE---ISSGDDSGKSSPDLSEHEEK 276
Query: 346 KVIEETE 366
V +T+
Sbjct: 277 AVEVDTD 283
>ref|XP_003620153.2| DNA-binding protein RHL1, putative [Medicago truncatula]
gi|657383095|gb|AES76371.2| DNA-binding protein RHL1,
putative [Medicago truncatula]
Length = 424
Score = 68.6 bits (166), Expect = 2e-09
Identities = 49/127 (38%), Positives = 63/127 (49%), Gaps = 6/127 (4%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGAGPEE------KFGGIKPREEKRKPQSPTDSLEXXXX 165
+LEFPKEL E K +HDFKGGAGAG G K + ++ +P+SP LE
Sbjct: 167 KLEFPKELYEGKQTEHDFKGGAGAGAGAGAASVVNHGVSKTKIKRPEPESPETPLE---- 222
Query: 166 XXXXXCPSPGITLKDGMEVTPVRQSARTSGRRFNFAEXXXXXXXXXXXXTDVDLSEIKGK 345
I LKD E+ PVRQSART + + FAE + DLSE + K
Sbjct: 223 ---EDLSDSEIELKDTTELVPVRQSARTVKKSYKFAE---ISSGDDSGKSSPDLSEHEEK 276
Query: 346 KVIEETE 366
V +T+
Sbjct: 277 AVEVDTD 283
>ref|XP_010935551.1| PREDICTED: DNA-binding protein RHL1-like [Elaeis guineensis]
Length = 405
Score = 67.4 bits (163), Expect = 4e-09
Identities = 49/140 (35%), Positives = 71/140 (50%), Gaps = 1/140 (0%)
Frame = +1
Query: 1 VRLEFPKELKEEKHV-DHDFKGGAGAGPEEKFGGIKPREEKRKPQSPTDSLEXXXXXXXX 177
++LEFPK L E H D DFKGGAGA EE G P ++ +P SP +E
Sbjct: 160 LKLEFPKNLNEGNHAADCDFKGGAGATVEESLRGNIPGKDYVEPFSPDTDMEGDMPEDSH 219
Query: 178 XCPSPGITLKDGMEVTPVRQSARTSGRRFNFAEXXXXXXXXXXXXTDVDLSEIKGKKVIE 357
+ K+ E TPVRQS RT+G++ N+AE +D ++ E+ +K+
Sbjct: 220 FKTEE--STKNLAEATPVRQSTRTAGKQLNYAE---SSSGDDSIASDAEVPEMMSEKI-- 272
Query: 358 ETEDAKTLKGIPNLKKTAKS 417
+ ED K I ++KT +S
Sbjct: 273 KIEDDFLEKKINPMQKTEES 292
>gb|KMT03733.1| hypothetical protein BVRB_8g188820 isoform A [Beta vulgaris subsp.
vulgaris]
Length = 282
Score = 66.6 bits (161), Expect = 7e-09
Identities = 43/95 (45%), Positives = 51/95 (53%), Gaps = 4/95 (4%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGA----GPEEKFGGIKPREEKRKPQSPTDSLEXXXXXX 171
RL+FPKEL E H++ DF+GGAGA K K EE+ P SP D +E
Sbjct: 164 RLDFPKELYERTHLESDFEGGAGATAMRSKNLKKVVSKSTEEESLPGSPVDEME---AEL 220
Query: 172 XXXCPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
C P KD TPVRQS RT+G+ FNFAE
Sbjct: 221 PERCQKP----KDVEYSTPVRQSGRTTGKTFNFAE 251
>ref|XP_010687257.1| PREDICTED: DNA-binding protein RHL1 isoform X3 [Beta vulgaris
subsp. vulgaris]
Length = 406
Score = 66.6 bits (161), Expect = 7e-09
Identities = 43/95 (45%), Positives = 51/95 (53%), Gaps = 4/95 (4%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGA----GPEEKFGGIKPREEKRKPQSPTDSLEXXXXXX 171
RL+FPKEL E H++ DF+GGAGA K K EE+ P SP D +E
Sbjct: 164 RLDFPKELYERTHLESDFEGGAGATAMRSKNLKKVVSKSTEEESLPGSPVDEME---AEL 220
Query: 172 XXXCPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
C P KD TPVRQS RT+G+ FNFAE
Sbjct: 221 PERCQKP----KDVEYSTPVRQSGRTTGKTFNFAE 251
>ref|XP_010687256.1| PREDICTED: DNA-binding protein RHL1 isoform X2 [Beta vulgaris
subsp. vulgaris]
Length = 414
Score = 66.6 bits (161), Expect = 7e-09
Identities = 43/95 (45%), Positives = 51/95 (53%), Gaps = 4/95 (4%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGA----GPEEKFGGIKPREEKRKPQSPTDSLEXXXXXX 171
RL+FPKEL E H++ DF+GGAGA K K EE+ P SP D +E
Sbjct: 164 RLDFPKELYERTHLESDFEGGAGATAMRSKNLKKVVSKSTEEESLPGSPVDEME---AEL 220
Query: 172 XXXCPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
C P KD TPVRQS RT+G+ FNFAE
Sbjct: 221 PERCQKP----KDVEYSTPVRQSGRTTGKTFNFAE 251
>ref|XP_010687255.1| PREDICTED: DNA-binding protein RHL1 isoform X1 [Beta vulgaris
subsp. vulgaris]
Length = 419
Score = 66.6 bits (161), Expect = 7e-09
Identities = 43/95 (45%), Positives = 51/95 (53%), Gaps = 4/95 (4%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGA----GPEEKFGGIKPREEKRKPQSPTDSLEXXXXXX 171
RL+FPKEL E H++ DF+GGAGA K K EE+ P SP D +E
Sbjct: 164 RLDFPKELYERTHLESDFEGGAGATAMRSKNLKKVVSKSTEEESLPGSPVDEME---AEL 220
Query: 172 XXXCPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
C P KD TPVRQS RT+G+ FNFAE
Sbjct: 221 PERCQKP----KDVEYSTPVRQSGRTTGKTFNFAE 251
>gb|KNA18952.1| hypothetical protein SOVF_065990 [Spinacia oleracea]
Length = 425
Score = 66.2 bits (160), Expect = 9e-09
Identities = 42/95 (44%), Positives = 52/95 (54%), Gaps = 4/95 (4%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGAGPEEKFGGI----KPREEKRKPQSPTDSLEXXXXXX 171
RL+FP+EL E KH + DF+GGAG+ P + + K EE+ P SP D E
Sbjct: 165 RLDFPEELYEGKHPEVDFEGGAGSTPTRRKDFMKVVTKSTEEESLPGSPIDETE------ 218
Query: 172 XXXCPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
P L D TPVRQS RT+G+RFNFAE
Sbjct: 219 -VISPERCQNLIDLENSTPVRQSGRTAGKRFNFAE 252
>gb|AGJ83755.1| DNA-binding protein RHL1-like protein, partial [Caragana
korshinskii]
Length = 280
Score = 66.2 bits (160), Expect = 9e-09
Identities = 47/123 (38%), Positives = 62/123 (50%), Gaps = 2/123 (1%)
Frame = +1
Query: 4 RLEFPKELKEEKHVDHDFKGGAGAGPEE--KFGGIKPREEKRKPQSPTDSLEXXXXXXXX 177
+LEFPKEL E + +HDFKGGAGAG G K + ++ + +SP LE
Sbjct: 168 KLEFPKELYEGQQAEHDFKGGAGAGAASVVNHGVPKIKIQRAEQESPKTPLE-------D 220
Query: 178 XCPSPGITLKDGMEVTPVRQSARTSGRRFNFAEXXXXXXXXXXXXTDVDLSEIKGKKVIE 357
I LKD E+ PVR SART+ R + FAE + DLSE + K V
Sbjct: 221 DLSDSEINLKDTKELVPVRHSARTAKRSYKFAE---VSSGDGSGESSPDLSEQEEKVVEV 277
Query: 358 ETE 366
+T+
Sbjct: 278 DTD 280
>ref|XP_009398296.1| PREDICTED: DNA-binding protein RHL1 [Musa acuminata subsp.
malaccensis]
Length = 427
Score = 64.3 bits (155), Expect = 3e-08
Identities = 41/93 (44%), Positives = 52/93 (55%), Gaps = 1/93 (1%)
Frame = +1
Query: 1 VRLEFPKELKEEKH-VDHDFKGGAGAGPEEKFGGIKPREEKRKPQSPTDSLEXXXXXXXX 177
++LEFPK L E K +D+DFKGGAGA E+ G KP KR+P SP E
Sbjct: 162 LQLEFPKYLSEGKDAIDYDFKGGAGATVGEQSAGNKP-VNKREPLSPETEFEDSLPDDSG 220
Query: 178 XCPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
PG TPVRQSART+G++ ++AE
Sbjct: 221 NKVEPGTI----NMATPVRQSARTAGKKLSYAE 249
>ref|XP_012476900.1| PREDICTED: DNA-binding protein RHL1 isoform X6 [Gossypium
raimondii]
Length = 383
Score = 63.5 bits (153), Expect = 6e-08
Identities = 41/92 (44%), Positives = 53/92 (57%)
Frame = +1
Query: 1 VRLEFPKELKEEKHVDHDFKGGAGAGPEEKFGGIKPREEKRKPQSPTDSLEXXXXXXXXX 180
VRL+FPKEL E + +++DFKGGAG K G PR E + Q+ T+SL+
Sbjct: 162 VRLDFPKELFEGEQMEYDFKGGAGVTSINKQG--IPRTEMK--QADTESLD---TESEDA 214
Query: 181 CPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
+LK ME TP R SAR +G+RF FAE
Sbjct: 215 LSDDETSLKVKMEATPTRHSARNAGKRFKFAE 246
>ref|XP_012476896.1| PREDICTED: DNA-binding protein RHL1 isoform X2 [Gossypium
raimondii]
Length = 450
Score = 63.5 bits (153), Expect = 6e-08
Identities = 41/92 (44%), Positives = 53/92 (57%)
Frame = +1
Query: 1 VRLEFPKELKEEKHVDHDFKGGAGAGPEEKFGGIKPREEKRKPQSPTDSLEXXXXXXXXX 180
VRL+FPKEL E + +++DFKGGAG K G PR E + Q+ T+SL+
Sbjct: 162 VRLDFPKELFEGEQMEYDFKGGAGVTSINKQG--IPRTEMK--QADTESLD---TESEDA 214
Query: 181 CPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
+LK ME TP R SAR +G+RF FAE
Sbjct: 215 LSDDETSLKVKMEATPTRHSARNAGKRFKFAE 246
>ref|XP_012476895.1| PREDICTED: DNA-binding protein RHL1 isoform X1 [Gossypium
raimondii]
Length = 457
Score = 63.5 bits (153), Expect = 6e-08
Identities = 41/92 (44%), Positives = 53/92 (57%)
Frame = +1
Query: 1 VRLEFPKELKEEKHVDHDFKGGAGAGPEEKFGGIKPREEKRKPQSPTDSLEXXXXXXXXX 180
VRL+FPKEL E + +++DFKGGAG K G PR E + Q+ T+SL+
Sbjct: 162 VRLDFPKELFEGEQMEYDFKGGAGVTSINKQG--IPRTEMK--QADTESLD---TESEDA 214
Query: 181 CPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
+LK ME TP R SAR +G+RF FAE
Sbjct: 215 LSDDETSLKVKMEATPTRHSARNAGKRFKFAE 246
>gb|KJB26814.1| hypothetical protein B456_004G262200 [Gossypium raimondii]
Length = 447
Score = 63.5 bits (153), Expect = 6e-08
Identities = 41/92 (44%), Positives = 53/92 (57%)
Frame = +1
Query: 1 VRLEFPKELKEEKHVDHDFKGGAGAGPEEKFGGIKPREEKRKPQSPTDSLEXXXXXXXXX 180
VRL+FPKEL E + +++DFKGGAG K G PR E + Q+ T+SL+
Sbjct: 162 VRLDFPKELFEGEQMEYDFKGGAGVTSINKQG--IPRTEMK--QADTESLD---TESEDA 214
Query: 181 CPSPGITLKDGMEVTPVRQSARTSGRRFNFAE 276
+LK ME TP R SAR +G+RF FAE
Sbjct: 215 LSDDETSLKVKMEATPTRHSARNAGKRFKFAE 246