BLASTX nr result
ID: Papaver29_contig00014065
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver29_contig00014065
(1433 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 806 0.0
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 804 0.0
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 792 0.0
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 656 0.0
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 654 0.0
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 651 0.0
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 620 e-175
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 619 e-174
sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 612 e-172
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 601 e-169
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 417 e-113
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 410 e-111
ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 399 e-108
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum] 397 e-107
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 395 e-107
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 393 e-106
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 393 e-106
ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 392 e-106
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 392 e-106
ref|XP_012076022.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 391 e-106
>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3
gi|6176562|gb|AAF05621.1|AF191772_1
(S)-N-methylcoclaurine 3'-hydroxylase [Papaver
somniferum]
Length = 481
Score = 806 bits (2082), Expect = 0.0
Identities = 405/425 (95%), Positives = 406/425 (95%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK
Sbjct: 57 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 116
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI
Sbjct: 117 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 176
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQ KDVADCLQGI
Sbjct: 177 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGI 236
Query: 891 YSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 712
YSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW
Sbjct: 237 YSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 296
Query: 711 ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 532
ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKE
Sbjct: 297 ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRR 356
Query: 531 XLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 352
LETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF
Sbjct: 357 ALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 416
Query: 351 GAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV 172
GAGRRICPGVPLATQFISLIV +LVQNFDWG PKGMDPSQLIMEEKFGLTLQKEPPLYIV
Sbjct: 417 GAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIV 476
Query: 171 PKTRD 157
PKTRD
Sbjct: 477 PKTRD 481
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 804 bits (2077), Expect = 0.0
Identities = 404/425 (95%), Positives = 406/425 (95%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK
Sbjct: 57 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 116
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
KLRKVCRT+LFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI
Sbjct: 117 KLRKVCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 176
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQ KDVADCLQGI
Sbjct: 177 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGI 236
Query: 891 YSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 712
YSVWGAMLKE KIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW
Sbjct: 237 YSVWGAMLKESKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 296
Query: 711 ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 532
ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKE
Sbjct: 297 ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRR 356
Query: 531 XLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 352
LETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF
Sbjct: 357 ALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 416
Query: 351 GAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV 172
GAGRRICPGVPLATQFISLIV +LVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV
Sbjct: 417 GAGRRICPGVPLATQFISLIVSSLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV 476
Query: 171 PKTRD 157
PKTRD
Sbjct: 477 PKTRD 481
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 792 bits (2046), Expect = 0.0
Identities = 395/425 (92%), Positives = 403/425 (94%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
LF+LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK
Sbjct: 62 LFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 121
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
KLRKVCR ELFTQKMIESQAE+RESKAMEMVE+LK+N G+EVKI EVVFGTLVNIFGNLI
Sbjct: 122 KLRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLI 181
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG+FDLFGQRKDVADCLQGI
Sbjct: 182 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 241
Query: 891 YSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 712
YSVWGAMLKERKIAK HNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW
Sbjct: 242 YSVWGAMLKERKIAKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 301
Query: 711 ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 532
ALSELTKNP+VTANMR ELLSVVGKRPVKESDIPNMPYLQAFVKE
Sbjct: 302 ALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRR 361
Query: 531 XLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 352
LETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKF+PERFLNSSIDFKGNDFELIPF
Sbjct: 362 ALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFAPERFLNSSIDFKGNDFELIPF 421
Query: 351 GAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV 172
GAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV
Sbjct: 422 GAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV 481
Query: 171 PKTRD 157
PK RD
Sbjct: 482 PKNRD 486
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1
gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 487
Score = 656 bits (1692), Expect = 0.0
Identities = 320/425 (75%), Positives = 364/425 (85%), Gaps = 1/425 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
+F+LK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C ETWK
Sbjct: 62 IFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWK 121
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
LRKVCRTELFTQKMIESQA VRE K EMVEYL K G EVKI EV+FGTLVNIFGNLI
Sbjct: 122 NLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLI 181
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FSQNIF+LGD +SGS E KE+LWRMLELGNSTNPADYFP LGKFDLFGQRK+VA+CL+GI
Sbjct: 182 FSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGI 241
Query: 891 YSVWGAMLKERKIAKQHNNSK-KNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIE 715
Y++WGAML+ERK+AK+ + K KNDFV++ LDSGL+D QINALLME+FGAGTETSASTIE
Sbjct: 242 YAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIE 301
Query: 714 WALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXX 535
WA++ELTKNP++TA +R E+ +VVG+R VKESD PN+PYL+A VKE
Sbjct: 302 WAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPR 361
Query: 534 XXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIP 355
LETC +LNYTIPK+CQIMVNAWGIGRDPK WTDPL FSPERFLNSS+DF+GNDF LIP
Sbjct: 362 RALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIP 421
Query: 354 FGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYI 175
FGAGRRICPG+P+A QFI+L+V T VQN DW LP GM LI+EEKFGLTLQKEPPL+I
Sbjct: 422 FGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFI 481
Query: 174 VPKTR 160
VPK+R
Sbjct: 482 VPKSR 486
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 654 bits (1686), Expect = 0.0
Identities = 319/425 (75%), Positives = 362/425 (85%), Gaps = 1/425 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
+F+LK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C ETWK
Sbjct: 63 IFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWK 122
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
LRKVCRTELFTQKMIESQA VRE K EMVEYL K G EVKI EV+FGTLVNIFGNLI
Sbjct: 123 NLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLI 182
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FSQNIF+LGD +SGS E KE+LWRMLELGNSTNPADYFP LGKFDLFGQRK+VA+CL+GI
Sbjct: 183 FSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGI 242
Query: 891 YSVWGAMLKERKIAKQHNN-SKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIE 715
Y++WGAML+ERK+AK+ + KNDFV++ LDSGL+D QINALLME+FGAGTETSASTIE
Sbjct: 243 YAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIE 302
Query: 714 WALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXX 535
WA++ELTKNP++TA +R EL +VVG+R VKESD PN+PYL+A VKE
Sbjct: 303 WAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPR 362
Query: 534 XXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIP 355
LETC +LNYTIPK+CQIMVNAWGIGRDPK W DPL FSPERFLNSS+DF+GNDF LIP
Sbjct: 363 RALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIP 422
Query: 354 FGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYI 175
FGAGRRICPG+P+A QFI+L+V T VQN DW LP GM LI+EEKFGLTLQKEPPL+I
Sbjct: 423 FGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFI 482
Query: 174 VPKTR 160
VPK+R
Sbjct: 483 VPKSR 487
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2
gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 488
Score = 651 bits (1679), Expect = 0.0
Identities = 318/425 (74%), Positives = 361/425 (84%), Gaps = 1/425 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
+F+LK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C ETWK
Sbjct: 63 IFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWK 122
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
LRKVCRTELFTQKMIESQA VRE K EMVEYL K G EVKI EV+FGTLVNIFGNLI
Sbjct: 123 NLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLI 182
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FSQNIF+LG +SGS E KE+LWRMLELGNSTNPADYFP LGKFDLFGQRK+VA+CL+GI
Sbjct: 183 FSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGI 242
Query: 891 YSVWGAMLKERKIAKQHNN-SKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIE 715
Y++WGAML+ERK+AK+ + KNDFV++ LDSGL+D QINALLME+FGAGTETSASTIE
Sbjct: 243 YAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIE 302
Query: 714 WALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXX 535
WA++ELTKNP++TA +R EL +VVG+R VKESD PN+PYL+A VKE
Sbjct: 303 WAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPR 362
Query: 534 XXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIP 355
LETC +LNYTIPK+CQIMVNAWGIGRDPK W DPL FSPERFLNSS+DF+GNDF LIP
Sbjct: 363 RALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIP 422
Query: 354 FGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYI 175
FGAGRRICPG+P+A QFI+L+V T VQN DW LP GM LI+EEKFGLTLQKEPPL+I
Sbjct: 423 FGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFI 482
Query: 174 VPKTR 160
VPK+R
Sbjct: 483 VPKSR 487
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 [Coptis japonica]
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 620 bits (1600), Expect = e-175
Identities = 302/424 (71%), Positives = 355/424 (83%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
LFSLKLGS+TVVVAS+P AA+EILKTHD++LSGRYVFQSFRVKEHVENSIVWSECN+ WK
Sbjct: 63 LFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWK 122
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
LRKVCRTELFT KMIESQ+E+RE+KA EMV++L+ G VKI EVVFGTLVNIFGNLI
Sbjct: 123 LLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLI 182
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FS+++F L D + GSVE+KEHLW++L++GNSTNPADYFP +GK DLFGQR+ VA+ LQ I
Sbjct: 183 FSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQI 242
Query: 891 YSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 712
Y VWG MLKER+ K + KNDFV++LL++GLDDQ+INALLME+FGAGTETSASTIEW
Sbjct: 243 YDVWGVMLKERRGTK--GSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW 300
Query: 711 ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 532
A++ELTK P V + +RLEL++VVG VKESD+P++PYLQAFVKE
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRR 360
Query: 531 XLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 352
LETC V+NYTIPKECQIMVNAW IGRDPK W DPL F PERFL+S +D+KGNDFELIPF
Sbjct: 361 ALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 420
Query: 351 GAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV 172
G GRRICPG+PLA+QF +LIV TLVQNF+W LP+GM S+L M+EKFGLTLQK+PPL IV
Sbjct: 421 GGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIV 480
Query: 171 PKTR 160
K R
Sbjct: 481 LKAR 484
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 619 bits (1597), Expect = e-174
Identities = 301/424 (70%), Positives = 353/424 (83%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
LFSLKLGS+TVVVAS+P AA+E+LKTHD++LSGRYVFQSFRVKEHVENSIVWSECN+ WK
Sbjct: 63 LFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWK 122
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
LRKVCRTELFT KMIESQ+E+RE KA EMV++L+ G VKI EVVFGTLVNIFGNLI
Sbjct: 123 LLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLI 182
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FS+++F L D + GSVE+KEHLW++LE+GNSTNPADYFP +GK+DLFGQR+ VAD LQ I
Sbjct: 183 FSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQI 242
Query: 891 YSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 712
Y VWG LKER+ K + NDFV++LL++GL+DQ+INALLME+FG GTETSASTIEW
Sbjct: 243 YDVWGINLKERRGMK--GSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEW 300
Query: 711 ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 532
A++ELTK P V + +RLEL++VVG VKESD+P++PYLQAFVKE
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRR 360
Query: 531 XLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 352
LETC V+NYTIPKECQIMVNAW IGRDPK W DPL F PERFL+S +D+KGNDFELIPF
Sbjct: 361 ALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 420
Query: 351 GAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV 172
G GRRICPG+PLA+QFISLIV TLVQNF+W LP+GM S+L M+EKFGLTLQK+PPL IV
Sbjct: 421 GGGRRICPGLPLASQFISLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIV 480
Query: 171 PKTR 160
K R
Sbjct: 481 LKAR 484
>sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
AltName: Full=Cytochrome P450 80BX
gi|388571234|gb|AFK73713.1| cytochrome P450 [Papaver
somniferum]
Length = 440
Score = 612 bits (1577), Expect = e-172
Identities = 301/372 (80%), Positives = 330/372 (88%), Gaps = 1/372 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
LF+LKLG++TVVVASTPLAASE+LK HDR L GRYVFQSFRVK HVENSIVW+ECNETWK
Sbjct: 63 LFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNETWK 122
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
KLRKVCRT+LFTQKMIE+QAEVRE K MEMV+YLKKN G EVKI EV+FGTLVN+FGNLI
Sbjct: 123 KLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLI 182
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FSQ+IFKLGDESSGS+EMK+H+WRMLELGNS NP DY P LG DLFGQRKDVADCLQG+
Sbjct: 183 FSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQGV 242
Query: 891 YSVWGAMLKERKIAK-QHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIE 715
Y VWGAMLKER+IAK Q N KNDFV++LLDSGLDDQQIN+LL+++F AGTET+ASTIE
Sbjct: 243 YGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTASTIE 302
Query: 714 WALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXX 535
WAL+ELTKNPQVTA++R ELLSVVGKR VKESDI N+PYLQAFVKE
Sbjct: 303 WALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLIPR 362
Query: 534 XXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIP 355
LETC+VLNYTIPKECQIMVNAWGIGRDPK WTDPLKFSP+RFLNSSIDFKGNDFELIP
Sbjct: 363 RALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFELIP 422
Query: 354 FGAGRRICPGVP 319
FGAGRRICPGVP
Sbjct: 423 FGAGRRICPGVP 434
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 601 bits (1550), Expect = e-169
Identities = 290/424 (68%), Positives = 350/424 (82%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
LF+LKLGS+TVVVAS+P AA+EILKT D++LSGRYVFQSFRV EHV NSIVWSECNE WK
Sbjct: 64 LFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNENWK 123
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
LRKVCRTELF+ KMIESQA +RE+KA++MV +L+K EVKI EVVF TLVNIFGNLI
Sbjct: 124 LLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLI 183
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FS+++F L D + GS E+KEHLW++L++GNSTNPADYFP +GKFDLFGQR++VA L+ I
Sbjct: 184 FSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVLKQI 243
Query: 891 YSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 712
Y VWG MLKER+ H ++NDF +LL++GLDDQ+INALLME+FGAGTET+ASTIEW
Sbjct: 244 YDVWGVMLKERRSITGH---RENDFANVLLNAGLDDQKINALLMELFGAGTETTASTIEW 300
Query: 711 ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 532
A++ELT N +V + +R EL++VVG + VKES+IP++PYLQA VKE
Sbjct: 301 AITELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKETLRLHPPTPLLLPRR 360
Query: 531 XLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 352
LETCKV+NYTIPKECQIMVNAW IGRDPK W DPL F PERF+NS++D+KGNDFELIPF
Sbjct: 361 ALETCKVMNYTIPKECQIMVNAWAIGRDPKTWDDPLTFKPERFMNSTVDYKGNDFELIPF 420
Query: 351 GAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV 172
G GRRICPG+PLA+QF+SLIV TLVQNFDW P+GM P+++ M+EKFGL LQK+PPL IV
Sbjct: 421 GGGRRICPGLPLASQFLSLIVATLVQNFDWSFPQGMTPNEVPMDEKFGLPLQKDPPLLIV 480
Query: 171 PKTR 160
K R
Sbjct: 481 LKAR 484
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 417 bits (1072), Expect = e-113
Identities = 205/425 (48%), Positives = 291/425 (68%), Gaps = 1/425 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 1252
L LK G E VVVAS+ +AA E+LK DR+LSGR+ S R+K ++E+S+VW++C + WK
Sbjct: 63 LMLLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWK 122
Query: 1251 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 1072
+RKV RTELF+ KM++ QA RE K E++++L + G +V A+V+FG+++NI G LI
Sbjct: 123 MVRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALI 182
Query: 1071 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGI 892
FS++++ D + ++ MK + +++ L N AD +P LG D G RK A C++ +
Sbjct: 183 FSKDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVKRM 242
Query: 891 YSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 712
W A++K+R+ H+ KNDF+++LLDSG D QI+A+L+E FG G++TS STIEW
Sbjct: 243 NESWAAIVKQRRKNDDHS---KNDFLQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTIEW 299
Query: 711 ALSELTKNPQVTANMRLELLSVVGK-RPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXX 535
A++EL +NP+ +R EL V+ + VKESD+PN+PYL A VKE
Sbjct: 300 AMAELLRNPEKLVKVREELDRVIRRSNNVKESDLPNLPYLHACVKETLRLHPPVTFLLPH 359
Query: 534 XXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIP 355
+ETC+++NYTIPK CQ+MVN + IGRD K W PL F PERFLNS +D++GNDF+ IP
Sbjct: 360 RAMETCQMMNYTIPKGCQLMVNTYAIGRDSKTWEKPLSFLPERFLNSELDYQGNDFQYIP 419
Query: 354 FGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYI 175
FGAGRRICPG+ LAT+ + LI+ +L+ FDW LP GM P +L M +KFGL LQK+ PL +
Sbjct: 420 FGAGRRICPGLSLATRVVRLILASLLHTFDWSLPDGMHPDELDMNDKFGLALQKDIPLVV 479
Query: 174 VPKTR 160
+PK R
Sbjct: 480 IPKLR 484
>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223549945|gb|EEF51432.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 410 bits (1054), Expect = e-111
Identities = 199/428 (46%), Positives = 287/428 (67%), Gaps = 4/428 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWSE-CNE 1261
L SL+LG++ +VVASTP +A+E+LK HDR+LS R++ ++ + H+ +IVW+ CN+
Sbjct: 70 LISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERVAIVWNPACND 129
Query: 1260 TWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 1081
WK LR +CRTELF+ K IESQA +RE K EM+E+L G V +AEVVFGT+ N
Sbjct: 130 HWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEVVFGTIFNTIS 189
Query: 1080 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCL 901
+L+FS ++ ++ G +K LW M+E+ S N A+++P L D G ++ + CL
Sbjct: 190 HLLFSTDLIGFENQIGG---VKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCL 246
Query: 900 QGIYSVWGAMLKERKIAKQHNNSK-KNDFVEILLDSGLDDQQINALLMEIFGAGTETSAS 724
+ ++ VW +KER+ H+++ K DF++I L +G DD QIN L+ME+ AGT+T+++
Sbjct: 247 KEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLSAGTDTTST 306
Query: 723 TIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXX 544
++EWA++EL KN + +R EL + K P+KES + +PYL A VKE
Sbjct: 307 SVEWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLRLHPPAPFL 366
Query: 543 XXXXXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFE 364
E C+V+NYTIPK+ Q++VN W IGRDP W DP F PERFL SS+D KG+DFE
Sbjct: 367 IPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLGSSLDVKGHDFE 426
Query: 363 LIPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPP 184
LIPFG+GRRICPG+P+AT+ +SL++ +L+ FDW LP G DP+++ M EKFG+TLQ E P
Sbjct: 427 LIPFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKVDMTEKFGITLQMEHP 486
Query: 183 LYIVPKTR 160
L I+PK +
Sbjct: 487 LLIIPKPK 494
>ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Populus euphratica]
Length = 492
Score = 399 bits (1024), Expect = e-108
Identities = 196/427 (45%), Positives = 285/427 (66%), Gaps = 3/427 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWS-ECNE 1261
L SLKLG + ++V S+ AA+EILK+HDR+LS RYVF++ + HV + SIVW+ +C++
Sbjct: 67 LISLKLGKQLLIVGSSKRAATEILKSHDRLLSARYVFKAALFENHVLDRISIVWATQCSD 126
Query: 1260 TWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 1081
WK LR +CR ELF+ K IESQA +RE K EMVE++ + G V I EVV + N
Sbjct: 127 GWKSLRALCRNELFSAKAIESQAVLREKKMGEMVEFIGRRKGEVVGIGEVVLAIVFNTIA 186
Query: 1080 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCL 901
NL+FS ++ L D+ + + +K +WRM++LG + N +D++P LG D G ++ +A C+
Sbjct: 187 NLLFSVDLIGLEDDGAAT-GLKSLMWRMMKLGATPNISDFYPILGGIDPQGLKRKMAVCV 245
Query: 900 QGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 721
++ +WG +KER+ K ++ ++DF+++ L +G +D QIN L +E+ AGT+T+A+T
Sbjct: 246 NQMFGIWGKYIKERR-EKHVHDGPRSDFLDVFLANGFEDLQINWLALELLSAGTDTTATT 304
Query: 720 IEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 541
+EWA++EL KN +V + E+ +KES + +PYL A V E
Sbjct: 305 VEWAIAELLKNKEVLKKVSEEIKRETDTNSLKESHVSQLPYLNACVNETLRLHPPVPFLI 364
Query: 540 XXXXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFEL 361
LETCKV++YTIP++ +++VN W +GRDP W DPL F PERFL S +DFKG+DFE
Sbjct: 365 PRRALETCKVMDYTIPRDSEVIVNVWAVGRDPSLWEDPLSFKPERFLGSDLDFKGHDFEF 424
Query: 360 IPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPL 181
+PFGAGRRICPG+P+A + + LI+ TL+ FDW LP G DP+ L M EKFG+TLQKE PL
Sbjct: 425 LPFGAGRRICPGLPMAAKQVHLIIATLLYYFDWSLPNGEDPATLDMSEKFGITLQKEQPL 484
Query: 180 YIVPKTR 160
+VP+ R
Sbjct: 485 LLVPRRR 491
>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 397 bits (1019), Expect = e-107
Identities = 200/421 (47%), Positives = 277/421 (65%)
Frame = -3
Query: 1422 LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLR 1243
L LG++ VAST AA EILKTHDR SGR V SFR+K H++ S+VW++C+E WK LR
Sbjct: 67 LWLGTQPTAVASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADCSEYWKLLR 126
Query: 1242 KVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 1063
K+ RTE+F+ KM++ Q VRE K E+VE+LK G VKI+++VFGTL+NI GN+IFS+
Sbjct: 127 KIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSK 186
Query: 1062 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGIYSV 883
++F+ + +M+ + ML +G + N A+++P +FDL G +K + +
Sbjct: 187 DVFRY--VGADGDKMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKM 244
Query: 882 WGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 703
W ++ERK+ + N + D +++LL + +D QINA+ +E FG G+ETS++T EWAL+
Sbjct: 245 WKGTVEERKVKR---NEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALA 301
Query: 702 ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXLE 523
EL KNP + A +R EL VVGK VKESD+P++PYLQA +KE +E
Sbjct: 302 ELIKNPHILAKVRQELDQVVGKSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVE 361
Query: 522 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAG 343
TC V+ YTIPK+ Q++VN + IGRDPK W +P F PERFL S +D+ G FE IPFGAG
Sbjct: 362 TCPVMGYTIPKDTQVLVNIYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHFEFIPFGAG 421
Query: 342 RRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKT 163
RR C G+PLAT+ I LIV LVQ DW LP G P L+M E L+L +P L ++PK+
Sbjct: 422 RRQCVGMPLATRTIPLIVSNLVQTLDWSLPDGKLPQDLVMNESLSLSLALDPTLTVIPKS 481
Query: 162 R 160
R
Sbjct: 482 R 482
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 395 bits (1014), Expect = e-107
Identities = 196/421 (46%), Positives = 278/421 (66%)
Frame = -3
Query: 1422 LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLR 1243
L LG++ V+AST AA EILKTHDR+ S R++ SFR+K H++ S+VWS+C + WK LR
Sbjct: 69 LWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLR 128
Query: 1242 KVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 1063
K+ RTE+F+ KM+++Q+ VRE K E++++L+ G VKI++ VFGTL+NI GN++FS+
Sbjct: 129 KIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSK 188
Query: 1062 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCLQGIYSV 883
++F GDE+ ++ + ML +G N A+++P L + DL G +K + + +
Sbjct: 189 DVFVYGDETDKG-GIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRVMKM 247
Query: 882 WGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 703
W +KER K + N + D +++LL + +D QINAL +E FG G+ETS++TIEW ++
Sbjct: 248 WEGTVKER---KANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIA 304
Query: 702 ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXLE 523
EL K+P+ A +R EL VVG +KESD+P +PYLQA +KE E
Sbjct: 305 ELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLLPRRAAE 364
Query: 522 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAG 343
TC+V+ YTIPK Q++VNA+ IGRDPK W DP F PERFL S +DF G ++ IPFG+G
Sbjct: 365 TCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQFIPFGSG 424
Query: 342 RRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKT 163
RR C G+PLAT+ I LIV +LV N+D+GLP G P L M E LTL +P L +VPK
Sbjct: 425 RRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAIDPSLCVVPKA 484
Query: 162 R 160
R
Sbjct: 485 R 485
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 393 bits (1009), Expect = e-106
Identities = 202/427 (47%), Positives = 273/427 (63%), Gaps = 3/427 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWS-ECNE 1261
L SL+LG++ +VVAS+P AA E+LKTHDR LSGRY+ S VK N SIV++ +CN
Sbjct: 612 LMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNT 671
Query: 1260 TWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 1081
WK LR +CR ELF+ K +ESQ E+RE K E+VE+L G VK+ ++VF T+ NI
Sbjct: 672 NWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILS 731
Query: 1080 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCL 901
N FS ++ DE +K+ + + E G + N +DY+P LG D+ G + +
Sbjct: 732 NKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMF 791
Query: 900 QGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 721
+ I + W +LKER+ ++ N S DF+E LL+ G +D QIN +++E+F AG ETS+ T
Sbjct: 792 ERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLT 850
Query: 720 IEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 541
+EWA++EL +N +R EL +VG+ PV+ES +P +PYLQA VKE
Sbjct: 851 VEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPPAPLLL 910
Query: 540 XXXXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFEL 361
ETC+V+ YTIPK+ QI VN W + RDPK W DPL F PERFL+S +DFKGNDFE
Sbjct: 911 PHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEY 970
Query: 360 IPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPL 181
IPFGAGRRICPG+ L + + LI+ T V F W LP MD +QL MEE +TL+KE PL
Sbjct: 971 IPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPL 1030
Query: 180 YIVPKTR 160
+VP+ R
Sbjct: 1031 RLVPRVR 1037
Score = 392 bits (1008), Expect = e-106
Identities = 198/427 (46%), Positives = 275/427 (64%), Gaps = 3/427 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWS---ECNE 1261
L SL+LG++ +VVAS+P AA E+LKTHDR LSGRYV S VK+ N + + EC
Sbjct: 1117 LMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTN 1176
Query: 1260 TWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 1081
WK LR +CRTE+F+ K +ES E+RE K ME+VE+L G VK+ ++VF T+ NI
Sbjct: 1177 NWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILS 1236
Query: 1080 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCL 901
N FS ++ E +K+ ++R ELG + N +D++P L +L G +K + L
Sbjct: 1237 NTFFSMDLCDFEREG-----LKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEAL 1291
Query: 900 QGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 721
I + W LKER+ K +S + D +E L+ +D QIN +++E+F AG +TS T
Sbjct: 1292 GRILATWEGTLKERRKQKNPGSSHR-DLLEAFLEIRFEDDQINQVILELFSAGADTSTLT 1350
Query: 720 IEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 541
IEWA+++L +NP V +R EL ++G+ PV+ES +P++PYLQA VKE
Sbjct: 1351 IEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACVKETLRLHPPAPLLL 1410
Query: 540 XXXXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFEL 361
+ETC+V+ YTIPK+ Q+ VN W +GRDPK W DPL F+PERFL+S ++FKGNDFE
Sbjct: 1411 PHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERFLDSKLEFKGNDFEY 1470
Query: 360 IPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPL 181
IPFGAGRRICPG+ L + + L++ TLV FDW LP MD +Q+ MEE +TL+KE PL
Sbjct: 1471 IPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKENPL 1530
Query: 180 YIVPKTR 160
+VPK R
Sbjct: 1531 RLVPKVR 1537
Score = 375 bits (962), Expect = e-101
Identities = 191/425 (44%), Positives = 273/425 (64%), Gaps = 3/425 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWS---ECNE 1261
L SL+LG++ +VVAS+ AA E+LKTHDR LSGRYV + V N + + CN
Sbjct: 72 LMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKVCNS 131
Query: 1260 TWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 1081
W+ L+ +CR ELF+ K +ES+ E+RE K ME+VE+L+K G VK+ ++V+ T+ NI
Sbjct: 132 DWRNLKAICRMELFSGKAMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILS 191
Query: 1080 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCL 901
N FS + V +K+ L ELG +TN D++P LG D+ G RK + +
Sbjct: 192 NKFFSIDFSDFEGRDVRGVLLKDLLNENAELG-ATNILDFYPILGGLDIQGIRKKLKEIF 250
Query: 900 QGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 721
+ I + W +LKER+ + H +S DF++ LL++G +D QIN ++ME+F AG ETS+ T
Sbjct: 251 RRIPTTWEDILKERRKQRIHGSSH-GDFLDALLETGFEDDQINHVIMELFFAGPETSSLT 309
Query: 720 IEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 541
+EWA++EL KN + EL ++G+ PV+ES +P++PYLQA VKE
Sbjct: 310 VEWAMAELIKNQDAMHKLCNELTQIIGESPVRESHLPHLPYLQACVKETLRLHPTGPLLL 369
Query: 540 XXXXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFEL 361
ETC+++ YTIPK+ I VN W +GRDP W DPL F PERFL+S ++FKGNDFE
Sbjct: 370 PHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWEDPLSFKPERFLDSKLEFKGNDFEY 429
Query: 360 IPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPL 181
IPFGAGRR+CPG+PLA + + +I+ T V+ FDW P MD +++ MEE+F +TL+KE PL
Sbjct: 430 IPFGAGRRMCPGMPLAARLVPMILATFVRLFDWSTPGDMDFAEIDMEERFVITLRKEQPL 489
Query: 180 YIVPK 166
++ K
Sbjct: 490 RLLKK 494
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 393 bits (1009), Expect = e-106
Identities = 202/427 (47%), Positives = 273/427 (63%), Gaps = 3/427 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWS-ECNE 1261
L SL+LG++ +VVAS+P AA E+LKTHDR LSGRY+ S VK N SIV++ +CN
Sbjct: 72 LMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNT 131
Query: 1260 TWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 1081
WK LR +CR ELF+ K +ESQ E+RE K E+VE+L G VK+ ++VF T+ NI
Sbjct: 132 NWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILS 191
Query: 1080 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCL 901
N FS ++ DE +K+ + + E G + N +DY+P LG D+ G + +
Sbjct: 192 NKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMF 251
Query: 900 QGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 721
+ I + W +LKER+ ++ N S DF+E LL+ G +D QIN +++E+F AG ETS+ T
Sbjct: 252 ERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLT 310
Query: 720 IEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 541
+EWA++EL +N +R EL +VG+ PV+ES +P +PYLQA VKE
Sbjct: 311 VEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPPAPLLL 370
Query: 540 XXXXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFEL 361
ETC+V+ YTIPK+ QI VN W + RDPK W DPL F PERFL+S +DFKGNDFE
Sbjct: 371 PHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEY 430
Query: 360 IPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPL 181
IPFGAGRRICPG+ L + + LI+ T V F W LP MD +QL MEE +TL+KE PL
Sbjct: 431 IPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPL 490
Query: 180 YIVPKTR 160
+VP+ R
Sbjct: 491 RLVPRVR 497
>ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 493
Score = 392 bits (1008), Expect = e-106
Identities = 198/427 (46%), Positives = 275/427 (64%), Gaps = 3/427 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWS---ECNE 1261
L SL+LG++ +VVAS+P AA E+LKTHDR LSGRYV S VK+ N + + EC
Sbjct: 72 LMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTN 131
Query: 1260 TWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 1081
WK LR +CRTE+F+ K +ES E+RE K ME+VE+L G VK+ ++VF T+ NI
Sbjct: 132 NWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILS 191
Query: 1080 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCL 901
N FS ++ E +K+ ++R ELG + N +D++P L +L G +K + L
Sbjct: 192 NTFFSMDLCDFEREG-----LKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEAL 246
Query: 900 QGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 721
I + W LKER+ K +S + D +E L+ +D QIN +++E+F AG +TS T
Sbjct: 247 GRILATWEGTLKERRKQKNPGSSHR-DLLEAFLEIRFEDDQINQVILELFSAGADTSTLT 305
Query: 720 IEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 541
IEWA+++L +NP V +R EL ++G+ PV+ES +P++PYLQA VKE
Sbjct: 306 IEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACVKETLRLHPPAPLLL 365
Query: 540 XXXXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFEL 361
+ETC+V+ YTIPK+ Q+ VN W +GRDPK W DPL F+PERFL+S ++FKGNDFE
Sbjct: 366 PHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERFLDSKLEFKGNDFEY 425
Query: 360 IPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPL 181
IPFGAGRRICPG+ L + + L++ TLV FDW LP MD +Q+ MEE +TL+KE PL
Sbjct: 426 IPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKENPL 485
Query: 180 YIVPKTR 160
+VPK R
Sbjct: 486 RLVPKVR 492
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 392 bits (1008), Expect = e-106
Identities = 202/427 (47%), Positives = 273/427 (63%), Gaps = 3/427 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWS-ECNE 1261
L SL+LG++ +VVAS+P AA E+LKTHDR LSGRY+ S VK N SIV++ +CN
Sbjct: 72 LMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNT 131
Query: 1260 TWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 1081
WK LR +CR ELF+ K +ESQ E+RE K E+VE+L G VK+ ++VF T+ NI
Sbjct: 132 NWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILS 191
Query: 1080 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCL 901
N FS ++ DE +K+ + + E G + N +DY+P LG D+ G + +
Sbjct: 192 NKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMF 251
Query: 900 QGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 721
+ I + W +LKER+ ++ N S DF+E LL+ G +D QIN +++E+F AG ETS+ T
Sbjct: 252 ERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLT 310
Query: 720 IEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 541
+EWA++EL +N +R EL +VG+ PV+ES +P +PYLQA VKE
Sbjct: 311 VEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPPAPLLL 370
Query: 540 XXXXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFEL 361
ETC+V+ YTIPK+ QI VN W + RDPK W DPL F PERFL+S +DFKGNDFE
Sbjct: 371 PHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEY 430
Query: 360 IPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPL 181
IPFGAGRRICPG+ L + + LI+ T V F W LP MD +QL MEE +TL+KE PL
Sbjct: 431 IPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPL 490
Query: 180 YIVPKTR 160
+VP+ R
Sbjct: 491 RLVPRVR 497
>ref|XP_012076022.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Jatropha curcas] gi|802796904|ref|XP_012092884.1|
PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase
isozyme 2 [Jatropha curcas]
Length = 502
Score = 391 bits (1005), Expect = e-106
Identities = 193/427 (45%), Positives = 277/427 (64%), Gaps = 3/427 (0%)
Frame = -3
Query: 1431 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSI--VWS-ECNE 1261
+FSL+LG + VVV S+P A+EIL+ DR LSGR+V ++ H + I VW+ C++
Sbjct: 77 IFSLRLGYQLVVVGSSPAVAAEILRKKDRFLSGRFVPKAIPRPSHELDRIALVWNPSCSD 136
Query: 1260 TWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 1081
WK LR + RTELF+ K IES+A +RE K EM+E+L G V + EVVF T+ N
Sbjct: 137 QWKSLRALSRTELFSAKAIESKAALREQKVSEMIEFLGIKQGQVVNVGEVVFATVFNSIS 196
Query: 1080 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQRKDVADCL 901
NL+FS++ DE S S +K +W+++ELG++ N AD++P DL G RK ++ CL
Sbjct: 197 NLLFSKDFISFEDEESVS-GLKGLMWKLMELGSAPNLADFYPIFSGLDLQGLRKKMSKCL 255
Query: 900 QGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 721
+ ++ +W +KER+ N K DF+++ + +G DD+QIN L+ E+F AGT+T+ +T
Sbjct: 256 KEMFDIWEIFIKERRERHARNGVVKEDFLDVFISNGFDDEQINWLIHELFSAGTDTTTTT 315
Query: 720 IEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 541
+EWA++EL KN + + EL + K + ES + PYL A +KE
Sbjct: 316 VEWAMAELLKNKEAMNKVCQELEREINKNSINESQVSQFPYLYACIKETFRLHPPAPFLG 375
Query: 540 XXXXLETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFEL 361
ETC+V+NYTIPK+ QI+VN W +GRDP W DPL F PERFL SS+D KG+DFEL
Sbjct: 376 HQPL-ETCEVMNYTIPKDSQIIVNIWAVGRDPLAWEDPLLFKPERFLESSLDLKGHDFEL 434
Query: 360 IPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPL 181
+PF +GRR+CPG+P+A +F+ LI+ +L+ FDW LP G DP+ L M +K+G+TLQKE PL
Sbjct: 435 LPFSSGRRMCPGLPMAIRFLPLILASLIHYFDWSLPGGEDPAMLDMNDKYGITLQKEKPL 494
Query: 180 YIVPKTR 160
+VP T+
Sbjct: 495 LLVPNTK 501