BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver29_contig00011207
         (1260 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_001154225.2| transcription initiation factor IIF subunit ...   127   3e-26
sp|Q9SU25.1|T2FA_ARATH RecName: Full=Transcription initiation fa...   127   3e-26
gb|AAR28013.1| mutant TFIIF-alpha [Arabidopsis thaliana]              125   8e-26
ref|XP_009769313.1| PREDICTED: transcription initiation factor I...   121   1e-24
ref|XP_002872663.1| mutant TFIIF-alpha [Arabidopsis lyrata subsp...   120   2e-24
ref|XP_002322538.2| transcription initiation factor IIF alpha su...   108   7e-21
ref|XP_002262798.3| PREDICTED: transcription initiation factor I...   108   1e-20
ref|XP_009757698.1| PREDICTED: transcription initiation factor I...   107   2e-20
ref|XP_009602254.1| PREDICTED: transcription initiation factor I...   107   2e-20
ref|XP_012069319.1| PREDICTED: transcription initiation factor I...   106   5e-20
ref|XP_010665737.1| PREDICTED: transcription initiation factor I...   105   8e-20
ref|XP_010665736.1| PREDICTED: transcription initiation factor I...   105   8e-20
ref|XP_007154011.1| hypothetical protein PHAVU_003G083500g [Phas...   105   8e-20
ref|XP_004293352.1| PREDICTED: transcription initiation factor I...   105   8e-20
ref|XP_014506009.1| PREDICTED: transcription initiation factor I...   105   1e-19
ref|XP_011008636.1| PREDICTED: transcription initiation factor I...   105   1e-19
ref|XP_010270094.1| PREDICTED: transcription initiation factor I...   105   1e-19
ref|XP_012080961.1| PREDICTED: transcription initiation factor I...   105   1e-19
ref|XP_002308775.1| transcription initiation factor IIF alpha su...   105   1e-19
ref|XP_006380878.1| hypothetical protein POPTR_0006s01070g [Popu...   105   1e-19

>ref|NP_001154225.2| transcription initiation factor IIF subunit alpha [Arabidopsis
           thaliana] gi|332657756|gb|AEE83156.1| transcription
           initiation factor IIF subunit alpha [Arabidopsis
           thaliana]
          Length = 506

 Score =  127 bits (318), Expect = 3e-26
 Identities = 94/227 (41%), Positives = 125/227 (55%), Gaps = 16/227 (7%)
 Frame = -1

Query: 738 DAKVANSAPLKTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQS---TGP 568
           D+K  +S+  K VK E D               +N  P   V +  A APA  S   TGP
Sbjct: 279 DSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNT-PTKAVKAEPASAPASSSSAATGP 337

Query: 567 VTEEEIRAVLVQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK------FVVL 406
           VTE+EIRAVL++   +T Q LV+  + RLK+KEDKNAFA IL +ISK+QK      FVVL
Sbjct: 338 VTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKISKIQKNAGSQNFVVL 397

Query: 405 RDKNAVLSRGPVSASTKAPADPSTGP-----ITEEEIRAVLLESALLTTQALVAEFWERL 241
           R+K         S   K     +  P     +TE+EIR VL+E   LTT  LV  F ERL
Sbjct: 398 REKCQPKPGKRESRVNKLNIRSNLQPRKMELVTEDEIRKVLMEKKQLTTLELVMRFKERL 457

Query: 240 KSKEEKNAFTAILRRISKIRKS--DEANYVVLRDNNAITSRDLVSSS 106
            + E+K++F+ IL++I+K++K+   E   VVLRDN    + DL   S
Sbjct: 458 TTTEDKDSFSHILKKIAKLQKNPGSEKFVVVLRDNVTPLASDLTRLS 504



 Score = 86.3 bits (212), Expect = 5e-14
 Identities = 64/192 (33%), Positives = 101/192 (52%), Gaps = 21/192 (10%)
 Frame = -1

Query: 519 TPQALVAESRERLKSKEDKNAFAAILSRISKVQ--KFVVLRDKNAVLSRG--PVSASTKA 352
           TP A  ++ + +L   + K   +++  ++      K  +  ++   +S+   P  A    
Sbjct: 263 TPPAKPSKGKRKLNDGDSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNTPTKAVKAE 322

Query: 351 PADP-------STGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRI 193
           PA         +TGP+TE+EIRAVL+E   +TTQ LV+ F  RLK+KE+KNAF  ILR+I
Sbjct: 323 PASAPASSSSAATGPVTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKI 382

Query: 192 SKIRK-SDEANYVVLRD---------NNAITSRDLVSSSTKAPADPSTGRVTEEEIRSVL 43
           SKI+K +   N+VVLR+          + +   ++ S+      +     VTE+EIR VL
Sbjct: 383 SKIQKNAGSQNFVVLREKCQPKPGKRESRVNKLNIRSNLQPRKME----LVTEDEIRKVL 438

Query: 42  LKSAPLATQALV 7
           ++   L T  LV
Sbjct: 439 MEKKQLTTLELV 450


>sp|Q9SU25.1|T2FA_ARATH RecName: Full=Transcription initiation factor IIF subunit alpha;
            Short=TFIIF-alpha; AltName: Full=General transcription
            factor IIF subunit 1; AltName: Full=Transcription
            initiation factor RAP74 homolog; Short=AtRAP74
            gi|5823572|emb|CAB53754.1| putative protein [Arabidopsis
            thaliana] gi|7267963|emb|CAB78304.1| putative protein
            [Arabidopsis thaliana]
          Length = 649

 Score =  127 bits (318), Expect = 3e-26
 Identities = 94/227 (41%), Positives = 125/227 (55%), Gaps = 16/227 (7%)
 Frame = -1

Query: 738  DAKVANSAPLKTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQS---TGP 568
            D+K  +S+  K VK E D               +N  P   V +  A APA  S   TGP
Sbjct: 422  DSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNT-PTKAVKAEPASAPASSSSAATGP 480

Query: 567  VTEEEIRAVLVQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK------FVVL 406
            VTE+EIRAVL++   +T Q LV+  + RLK+KEDKNAFA IL +ISK+QK      FVVL
Sbjct: 481  VTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKISKIQKNAGSQNFVVL 540

Query: 405  RDKNAVLSRGPVSASTKAPADPSTGP-----ITEEEIRAVLLESALLTTQALVAEFWERL 241
            R+K         S   K     +  P     +TE+EIR VL+E   LTT  LV  F ERL
Sbjct: 541  REKCQPKPGKRESRVNKLNIRSNLQPRKMELVTEDEIRKVLMEKKQLTTLELVMRFKERL 600

Query: 240  KSKEEKNAFTAILRRISKIRKS--DEANYVVLRDNNAITSRDLVSSS 106
             + E+K++F+ IL++I+K++K+   E   VVLRDN    + DL   S
Sbjct: 601  TTTEDKDSFSHILKKIAKLQKNPGSEKFVVVLRDNVTPLASDLTRLS 647



 Score = 86.7 bits (213), Expect = 4e-14
 Identities = 36/58 (62%), Positives = 46/58 (79%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MS  L L  SC GCGS SDLYGS+C+HMTLCL CG+ M +N++KC++CGT +TRL R+
Sbjct: 1    MSNCLQLNTSCVGCGSQSDLYGSSCRHMTLCLKCGRTMAQNKSKCHECGTVVTRLIRE 58



 Score = 86.3 bits (212), Expect = 5e-14
 Identities = 64/192 (33%), Positives = 101/192 (52%), Gaps = 21/192 (10%)
 Frame = -1

Query: 519 TPQALVAESRERLKSKEDKNAFAAILSRISKVQ--KFVVLRDKNAVLSRG--PVSASTKA 352
           TP A  ++ + +L   + K   +++  ++      K  +  ++   +S+   P  A    
Sbjct: 406 TPPAKPSKGKRKLNDGDSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNTPTKAVKAE 465

Query: 351 PADP-------STGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRI 193
           PA         +TGP+TE+EIRAVL+E   +TTQ LV+ F  RLK+KE+KNAF  ILR+I
Sbjct: 466 PASAPASSSSAATGPVTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKI 525

Query: 192 SKIRK-SDEANYVVLRD---------NNAITSRDLVSSSTKAPADPSTGRVTEEEIRSVL 43
           SKI+K +   N+VVLR+          + +   ++ S+      +     VTE+EIR VL
Sbjct: 526 SKIQKNAGSQNFVVLREKCQPKPGKRESRVNKLNIRSNLQPRKME----LVTEDEIRKVL 581

Query: 42  LKSAPLATQALV 7
           ++   L T  LV
Sbjct: 582 MEKKQLTTLELV 593


>gb|AAR28013.1| mutant TFIIF-alpha [Arabidopsis thaliana]
          Length = 640

 Score =  125 bits (314), Expect = 8e-26
 Identities = 91/215 (42%), Positives = 121/215 (56%), Gaps = 16/215 (7%)
 Frame = -1

Query: 738  DAKVANSAPLKTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQS---TGP 568
            D+K  +S+  K VK E D               +N  P   V +  A APA  S   TGP
Sbjct: 422  DSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNT-PTKAVKAEPASAPASSSSAATGP 480

Query: 567  VTEEEIRAVLVQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK------FVVL 406
            VTE+EIRAVL++   +T Q LV+  + RLK+KEDKNAFA IL +ISK+QK      FVVL
Sbjct: 481  VTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKISKIQKNAGSQNFVVL 540

Query: 405  RDKNAVLSRGPVSASTKAPADPSTGP-----ITEEEIRAVLLESALLTTQALVAEFWERL 241
            R+K         S   K     +  P     +TE+EIR VL+E   LTT  LV  F ERL
Sbjct: 541  REKCQPKPGKRESRVNKLNIRSNLQPRKMELVTEDEIRKVLMEKKQLTTLELVMRFKERL 600

Query: 240  KSKEEKNAFTAILRRISKIRKS--DEANYVVLRDN 142
             + E+K++F+ IL++I+K++K+   E   VVLRDN
Sbjct: 601  TTTEDKDSFSHILKKITKLQKNPGSEKFVVVLRDN 635



 Score = 86.7 bits (213), Expect = 4e-14
 Identities = 36/58 (62%), Positives = 46/58 (79%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MS  L L  SC GCGS SDLYGS+C+HMTLCL CG+ M +N++KC++CGT +TRL R+
Sbjct: 1    MSNCLQLNTSCVGCGSQSDLYGSSCRHMTLCLKCGRTMAQNKSKCHECGTVVTRLIRE 58



 Score = 86.3 bits (212), Expect = 5e-14
 Identities = 64/192 (33%), Positives = 101/192 (52%), Gaps = 21/192 (10%)
 Frame = -1

Query: 519 TPQALVAESRERLKSKEDKNAFAAILSRISKVQ--KFVVLRDKNAVLSRG--PVSASTKA 352
           TP A  ++ + +L   + K   +++  ++      K  +  ++   +S+   P  A    
Sbjct: 406 TPPAKPSKGKRKLNDGDSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNTPTKAVKAE 465

Query: 351 PADP-------STGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRI 193
           PA         +TGP+TE+EIRAVL+E   +TTQ LV+ F  RLK+KE+KNAF  ILR+I
Sbjct: 466 PASAPASSSSAATGPVTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKI 525

Query: 192 SKIRK-SDEANYVVLRD---------NNAITSRDLVSSSTKAPADPSTGRVTEEEIRSVL 43
           SKI+K +   N+VVLR+          + +   ++ S+      +     VTE+EIR VL
Sbjct: 526 SKIQKNAGSQNFVVLREKCQPKPGKRESRVNKLNIRSNLQPRKME----LVTEDEIRKVL 581

Query: 42  LKSAPLATQALV 7
           ++   L T  LV
Sbjct: 582 MEKKQLTTLELV 593


>ref|XP_009769313.1| PREDICTED: transcription initiation factor IIF subunit alpha-like
           [Nicotiana sylvestris]
          Length = 225

 Score =  121 bits (303), Expect = 1e-24
 Identities = 77/181 (42%), Positives = 111/181 (61%), Gaps = 16/181 (8%)
 Frame = -1

Query: 639 NNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQGAPLTPQALVAESRERLKSKEDKN 460
           NN + L + AS   K     +TGPVTE+EIRA L+Q   +T  +LV++ + RLK +EDK 
Sbjct: 24  NNSV-LIKCASPFPKTTQSPATGPVTEDEIRAFLMQRKQVTKLSLVSKFKSRLKCREDKI 82

Query: 459 AFAAILSRISKVQK-----FVVLRDKNAVLSRGPVSAS-----------TKAPADPSTGP 328
           AFA +L RISK+QK     +VVL+D   V      +A+            K     +TGP
Sbjct: 83  AFANVLRRISKIQKTGTASYVVLQDAIPVEDESSSTANHSFLIKRASPFPKTAQSLATGP 142

Query: 327 ITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRISKIRKSDEANYVVLR 148
           +TE+EIRA LL+   +TT  LV++F  RL+ +E+K AF  +L+RI+KI+K+   +YVVLR
Sbjct: 143 VTEDEIRAFLLQRKSVTTLHLVSKFKSRLQRREDKAAFADVLKRITKIQKTGTTSYVVLR 202

Query: 147 D 145
           +
Sbjct: 203 E 203



 Score = 89.4 bits (220), Expect = 6e-15
 Identities = 56/132 (42%), Positives = 80/132 (60%), Gaps = 13/132 (9%)
 Frame = -1

Query: 357 KAPADPSTGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRISKIRK 178
           K    P+TGP+TE+EIRA L++   +T  +LV++F  RLK +E+K AF  +LRRISKI+K
Sbjct: 37  KTTQSPATGPVTEDEIRAFLMQRKQVTKLSLVSKFKSRLKCREDKIAFANVLRRISKIQK 96

Query: 177 SDEANYVVLRDNNAITSRDLVSSST-------------KAPADPSTGRVTEEEIRSVLLK 37
           +  A+YVVL+D  AI   D  SS+              K     +TG VTE+EIR+ LL+
Sbjct: 97  TGTASYVVLQD--AIPVEDESSSTANHSFLIKRASPFPKTAQSLATGPVTEDEIRAFLLQ 154

Query: 36  SAPLATQALVAE 1
              + T  LV++
Sbjct: 155 RKSVTTLHLVSK 166


>ref|XP_002872663.1| mutant TFIIF-alpha [Arabidopsis lyrata subsp. lyrata]
            gi|297318500|gb|EFH48922.1| mutant TFIIF-alpha
            [Arabidopsis lyrata subsp. lyrata]
          Length = 619

 Score =  120 bits (301), Expect = 2e-24
 Identities = 86/209 (41%), Positives = 121/209 (57%), Gaps = 11/209 (5%)
 Frame = -1

Query: 738  DAKVANSAPLKTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQS---TGP 568
            D+K  +S+  K VK E D               +N  P   V +  A APA  S   TGP
Sbjct: 421  DSKKPSSSVQKKVKTENDPKSSVKEERANTVSKSNT-PTKAVKAEPASAPAASSSAATGP 479

Query: 567  VTEEEIRAVLVQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK------FVVL 406
            VTE+EIRAVL++   +T Q LV+  + RLK+KEDKNAFA IL +ISK+QK      FVVL
Sbjct: 480  VTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKISKIQKNAGSQNFVVL 539

Query: 405  RDKNAVLSRGPVSASTKAPADPSTGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEE 226
            R+          S S + P       +TE+E+R+VL+E+  LTT  LV  F ERL ++EE
Sbjct: 540  RENCE-------SCSNRQPR--KMEHVTEDELRSVLMETKQLTTLELVMRFKERLTTREE 590

Query: 225  KNAFTAILRRISKIRKSDEA--NYVVLRD 145
            K+ F  IL++I+K++K+  +   ++VLR+
Sbjct: 591  KDTFAHILKKIAKLQKNAGSLKYFLVLRE 619



 Score = 97.4 bits (241), Expect = 2e-17
 Identities = 76/215 (35%), Positives = 112/215 (52%), Gaps = 26/215 (12%)
 Frame = -1

Query: 573 GPVTEEEIRAVL----VQGAPL----------TPQALVAESRERLKSKEDKNAFAAILSR 436
           GPVT  + + V     V+ AP           TP A  ++ + +L   + K   +++  +
Sbjct: 373 GPVTNSKQKEVAKEEPVENAPAKPTPSGPSRGTPPAKPSKGKRKLNDGDSKKPSSSVQKK 432

Query: 435 ISKVQ--KFVVLRDKNAVLSRG--PVSASTKAPADP-------STGPITEEEIRAVLLES 289
           +      K  V  ++   +S+   P  A    PA         +TGP+TE+EIRAVL+E 
Sbjct: 433 VKTENDPKSSVKEERANTVSKSNTPTKAVKAEPASAPAASSSAATGPVTEDEIRAVLMEK 492

Query: 288 ALLTTQALVAEFWERLKSKEEKNAFTAILRRISKIRK-SDEANYVVLRDNNAITSRDLVS 112
             +TTQ LV+ F  RLK+KE+KNAF  ILR+ISKI+K +   N+VVLR+N         S
Sbjct: 493 KQVTTQDLVSRFKARLKTKEDKNAFANILRKISKIQKNAGSQNFVVLREN-------CES 545

Query: 111 SSTKAPADPSTGRVTEEEIRSVLLKSAPLATQALV 7
            S + P       VTE+E+RSVL+++  L T  LV
Sbjct: 546 CSNRQPR--KMEHVTEDELRSVLMETKQLTTLELV 578



 Score = 92.8 bits (229), Expect = 5e-16
 Identities = 40/58 (68%), Positives = 47/58 (81%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MS  L LKP+C GCGS SDLYGS+C+HMTLCL CG+ M EN+AKC DCGT +TRL R+
Sbjct: 1    MSNCLQLKPACMGCGSQSDLYGSSCRHMTLCLKCGRTMAENKAKCLDCGTVLTRLIRE 58


>ref|XP_002322538.2| transcription initiation factor IIF alpha subunit family protein
            [Populus trichocarpa] gi|550320589|gb|EEF04299.2|
            transcription initiation factor IIF alpha subunit family
            protein [Populus trichocarpa]
          Length = 540

 Score =  108 bits (271), Expect = 7e-21
 Identities = 46/58 (79%), Positives = 51/58 (87%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MSFDL LKPSC GCGST+DLYGSNCKHMTLCLNCGK M ENR KC+DCGT ++RL R+
Sbjct: 1    MSFDLLLKPSCSGCGSTTDLYGSNCKHMTLCLNCGKTMAENRGKCFDCGTTVSRLIRE 58



 Score = 99.0 bits (245), Expect = 8e-18
 Identities = 69/169 (40%), Positives = 85/169 (50%), Gaps = 5/169 (2%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK+KD   PKEE  D +                          +G+DAK +N AP+
Sbjct: 375 PVLAPKQKDV-VPKEEAADISPAKPTPSGSAKGTPSTSKSAKGKRKLNGEDAKSSNGAPV 433

Query: 708 KTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQG 529
           K VK E +              G     +   +S         STGPVTEEEIRAVL+Q 
Sbjct: 434 KKVKTENEVKPAVKEESSPATKGTATPKVTPPSSKTGSTSG--STGPVTEEEIRAVLLQN 491

Query: 528 APLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLRDK 397
            P+T Q LVA  + RL++ EDK AFA IL RISK+QK     +VVLRDK
Sbjct: 492 GPVTTQDLVARFKSRLRTPEDKKAFADILRRISKIQKTSGSNYVVLRDK 540



 Score = 89.7 bits (221), Expect = 5e-15
 Identities = 52/137 (37%), Positives = 86/137 (62%), Gaps = 7/137 (5%)
 Frame = -1

Query: 534 QGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQKFV-VLRDKNAVLSRGPVSAST 358
           +G P T ++  A+ + +L  ++ K++  A + ++    +    ++++++  ++G  +   
Sbjct: 405 KGTPSTSKS--AKGKRKLNGEDAKSSNGAPVKKVKTENEVKPAVKEESSPATKGTATPKV 462

Query: 357 KAPADP------STGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRR 196
             P+        STGP+TEEEIRAVLL++  +TTQ LVA F  RL++ E+K AF  ILRR
Sbjct: 463 TPPSSKTGSTSGSTGPVTEEEIRAVLLQNGPVTTQDLVARFKSRLRTPEDKKAFADILRR 522

Query: 195 ISKIRKSDEANYVVLRD 145
           ISKI+K+  +NYVVLRD
Sbjct: 523 ISKIQKTSGSNYVVLRD 539


>ref|XP_002262798.3| PREDICTED: transcription initiation factor IIF subunit alpha [Vitis
            vinifera] gi|297741818|emb|CBI33123.3| unnamed protein
            product [Vitis vinifera]
          Length = 539

 Score =  108 bits (270), Expect = 1e-20
 Identities = 46/58 (79%), Positives = 50/58 (86%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MSFDL LKPSC GCGST+DLYGSNCKHMTLCL+CGK M EN  KCYDCGT +TRL R+
Sbjct: 1    MSFDLMLKPSCSGCGSTTDLYGSNCKHMTLCLSCGKTMAENHGKCYDCGTPVTRLIRE 58



 Score = 94.0 bits (232), Expect = 2e-16
 Identities = 70/169 (41%), Positives = 83/169 (49%), Gaps = 5/169 (2%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK+KD   PKEE  D +                           GDD K  N  P 
Sbjct: 376 PVLAPKQKDV--PKEEPADNSPMKSATASARSTPPTSKSSKGKRKSN-GDDMKTTNGGPP 432

Query: 708 KTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQG 529
           K VK E +                   P    +S   K  +  S GPVTE+EIRAVL+Q 
Sbjct: 433 KKVKTENESKSSAKDEGPSPSKVT-ATPKGTPSS-SVKTGSTSSAGPVTEDEIRAVLLQK 490

Query: 528 APLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLRDK 397
           AP+T Q LVA+ + RLKS+EDK AFA IL RISK+QK     +VVLRDK
Sbjct: 491 APVTTQDLVAKFKARLKSQEDKKAFADILRRISKIQKTNGPNYVVLRDK 539



 Score = 91.3 bits (225), Expect = 2e-15
 Identities = 48/79 (60%), Positives = 61/79 (77%)
 Frame = -1

Query: 381 RGPVSASTKAPADPSTGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAIL 202
           +G  S+S K  +  S GP+TE+EIRAVLL+ A +TTQ LVA+F  RLKS+E+K AF  IL
Sbjct: 460 KGTPSSSVKTGSTSSAGPVTEDEIRAVLLQKAPVTTQDLVAKFKARLKSQEDKKAFADIL 519

Query: 201 RRISKIRKSDEANYVVLRD 145
           RRISKI+K++  NYVVLRD
Sbjct: 520 RRISKIQKTNGPNYVVLRD 538


>ref|XP_009757698.1| PREDICTED: transcription initiation factor IIF subunit alpha
           [Nicotiana sylvestris]
          Length = 535

 Score =  107 bits (268), Expect = 2e-20
 Identities = 72/170 (42%), Positives = 94/170 (55%), Gaps = 6/170 (3%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK K+   PKEE +DT++                         +GDD+K  N APL
Sbjct: 372 PVLAPKPKEA--PKEEPIDTSSPSKPSASGPTRGTPTSKSAKTKRKANGDDSKPTNGAPL 429

Query: 708 KTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQG 529
           K VK ETD               N+V+P  + AS  +K     +TGPVTEEEIRAVL+Q 
Sbjct: 430 KKVKTETDAKPGKDETPSTAK--NSVLP--KGASPSSKTAPSPATGPVTEEEIRAVLLQR 485

Query: 528 APLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK------FVVLRDK 397
            P+T Q LV++ + RLK KEDK AFA +L +ISK+QK      +VVLR++
Sbjct: 486 KPVTTQDLVSQFKSRLKCKEDKAAFADVLRKISKIQKTGTGTSYVVLRER 535



 Score = 96.3 bits (238), Expect = 5e-17
 Identities = 41/58 (70%), Positives = 47/58 (81%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MS DL LKPSC  CGSTS+LYGS CKH+TLC++CGK M E R KCY+CGT ITRL R+
Sbjct: 1    MSIDLVLKPSCSRCGSTSELYGSTCKHLTLCMSCGKNMAETRDKCYECGTPITRLIRE 58



 Score = 87.8 bits (216), Expect = 2e-14
 Identities = 55/132 (41%), Positives = 81/132 (61%), Gaps = 7/132 (5%)
 Frame = -1

Query: 519 TPQALVAESRERLKSKEDKNAFAAILSRISKVQKFVVLRD------KNAVLSRGPVSAST 358
           TP +  A+++ +    + K    A L ++         +D      KN+VL +G   +S 
Sbjct: 404 TPTSKSAKTKRKANGDDSKPTNGAPLKKVKTETDAKPGKDETPSTAKNSVLPKGASPSSK 463

Query: 357 KAPADPSTGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRISKIRK 178
            AP+ P+TGP+TEEEIRAVLL+   +TTQ LV++F  RLK KE+K AF  +LR+ISKI+K
Sbjct: 464 TAPS-PATGPVTEEEIRAVLLQRKPVTTQDLVSQFKSRLKCKEDKAAFADVLRKISKIQK 522

Query: 177 SDE-ANYVVLRD 145
           +    +YVVLR+
Sbjct: 523 TGTGTSYVVLRE 534


>ref|XP_009602254.1| PREDICTED: transcription initiation factor IIF subunit alpha
           [Nicotiana tomentosiformis]
          Length = 535

 Score =  107 bits (268), Expect = 2e-20
 Identities = 72/170 (42%), Positives = 94/170 (55%), Gaps = 6/170 (3%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK K+   PKEE +DT++                         +GDD+K  N APL
Sbjct: 372 PVLAPKPKEA--PKEEPIDTSSPSKPSASGPTRGTPTSKPAKTKRKANGDDSKPTNGAPL 429

Query: 708 KTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQG 529
           K VK ETD               N+V+P  + AS  +K     +TGPVTEEEIRAVL+Q 
Sbjct: 430 KKVKTETDAKPGKDETPATAK--NSVLP--KGASPSSKTAPSPATGPVTEEEIRAVLLQR 485

Query: 528 APLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK------FVVLRDK 397
            P+T Q LV++ + RLK KEDK AFA +L +ISK+QK      +VVLR++
Sbjct: 486 KPVTTQDLVSQFKSRLKCKEDKAAFADVLRKISKIQKTGTGTSYVVLRER 535



 Score = 96.3 bits (238), Expect = 5e-17
 Identities = 41/58 (70%), Positives = 47/58 (81%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MS DL LKPSC  CGSTS+LYGS CKH+TLC++CGK M E R KCY+CGT ITRL R+
Sbjct: 1    MSIDLVLKPSCSRCGSTSELYGSTCKHLTLCMSCGKNMAETRDKCYECGTPITRLIRE 58



 Score = 87.8 bits (216), Expect = 2e-14
 Identities = 55/132 (41%), Positives = 81/132 (61%), Gaps = 7/132 (5%)
 Frame = -1

Query: 519 TPQALVAESRERLKSKEDKNAFAAILSRISKVQKFVVLRD------KNAVLSRGPVSAST 358
           TP +  A+++ +    + K    A L ++         +D      KN+VL +G   +S 
Sbjct: 404 TPTSKPAKTKRKANGDDSKPTNGAPLKKVKTETDAKPGKDETPATAKNSVLPKGASPSSK 463

Query: 357 KAPADPSTGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRISKIRK 178
            AP+ P+TGP+TEEEIRAVLL+   +TTQ LV++F  RLK KE+K AF  +LR+ISKI+K
Sbjct: 464 TAPS-PATGPVTEEEIRAVLLQRKPVTTQDLVSQFKSRLKCKEDKAAFADVLRKISKIQK 522

Query: 177 SDE-ANYVVLRD 145
           +    +YVVLR+
Sbjct: 523 TGTGTSYVVLRE 534


>ref|XP_012069319.1| PREDICTED: transcription initiation factor IIF subunit alpha
            [Jatropha curcas] gi|802578042|ref|XP_012069320.1|
            PREDICTED: transcription initiation factor IIF subunit
            alpha [Jatropha curcas] gi|802578044|ref|XP_012069321.1|
            PREDICTED: transcription initiation factor IIF subunit
            alpha [Jatropha curcas] gi|802578046|ref|XP_012069322.1|
            PREDICTED: transcription initiation factor IIF subunit
            alpha [Jatropha curcas] gi|643733570|gb|KDP40450.1|
            hypothetical protein JCGZ_03950 [Jatropha curcas]
          Length = 533

 Score =  106 bits (264), Expect = 5e-20
 Identities = 45/58 (77%), Positives = 48/58 (82%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MSFDL LKPSC GCGST +LYGSNCKHMTLCL CGK M ENR KCYDCG  +TRL R+
Sbjct: 1    MSFDLMLKPSCSGCGSTQELYGSNCKHMTLCLTCGKTMAENRGKCYDCGATVTRLIRE 58



 Score = 97.1 bits (240), Expect = 3e-17
 Identities = 71/169 (42%), Positives = 85/169 (50%), Gaps = 5/169 (2%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK+KD   PKEE VD +                          SGDDAK ++ AP 
Sbjct: 373 PVLAPKQKD--APKEEPVDNSPAKPMPSSSAKGTPSNSKSAKGKRKLSGDDAKTSSGAPQ 430

Query: 708 KTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQG 529
           K VK E D               +   P  + A+   K     ST PVTEEEIRAVL+Q 
Sbjct: 431 KKVKAENDLKPTVKEEHAPTAKSS---PTPKGATSSLK---PGSTSPVTEEEIRAVLLQN 484

Query: 528 APLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLRDK 397
            P+T Q LVA  + RLK  EDK AFA IL RISK+QK     +VVLR++
Sbjct: 485 GPVTTQDLVARFKSRLKGSEDKKAFAEILRRISKIQKTSGSNYVVLRER 533



 Score = 82.8 bits (203), Expect = 6e-13
 Identities = 54/153 (35%), Positives = 80/153 (52%), Gaps = 3/153 (1%)
 Frame = -1

Query: 594 APADQSTGPVTEEEIRAVLVQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQ-- 421
           AP ++       + + +   +G P   ++  A+ + +L   + K +  A   ++      
Sbjct: 382 APKEEPVDNSPAKPMPSSSAKGTPSNSKS--AKGKRKLSGDDAKTSSGAPQKKVKAENDL 439

Query: 420 KFVVLRDKNAVLSRGPVSASTKAPADP-STGPITEEEIRAVLLESALLTTQALVAEFWER 244
           K  V  +        P      +   P ST P+TEEEIRAVLL++  +TTQ LVA F  R
Sbjct: 440 KPTVKEEHAPTAKSSPTPKGATSSLKPGSTSPVTEEEIRAVLLQNGPVTTQDLVARFKSR 499

Query: 243 LKSKEEKNAFTAILRRISKIRKSDEANYVVLRD 145
           LK  E+K AF  ILRRISKI+K+  +NYVVLR+
Sbjct: 500 LKGSEDKKAFAEILRRISKIQKTSGSNYVVLRE 532


>ref|XP_010665737.1| PREDICTED: transcription initiation factor IIF subunit alpha isoform
            X2 [Beta vulgaris subsp. vulgaris]
          Length = 539

 Score =  105 bits (262), Expect = 8e-20
 Identities = 44/58 (75%), Positives = 50/58 (86%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MSFDL LKPSCGGCG T+DLYGSNCKH+TLC +CGK M ENRAKCY+CG  +TRL R+
Sbjct: 1    MSFDLTLKPSCGGCGVTTDLYGSNCKHLTLCSSCGKTMAENRAKCYECGATLTRLIRE 58



 Score = 80.5 bits (197), Expect = 3e-12
 Identities = 41/75 (54%), Positives = 52/75 (69%)
 Frame = -1

Query: 372 VSASTKAPADPSTGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRI 193
           + AST     P+ GP+ EEEIRAVLL+   +TT  LVA F  RLK+ E+K AF  IL++I
Sbjct: 463 LKASTPVQGTPAQGPVAEEEIRAVLLQRTQITTNDLVAVFKARLKTSEDKRAFAEILKKI 522

Query: 192 SKIRKSDEANYVVLR 148
           S+I+K D  NYVVLR
Sbjct: 523 SRIQKCDNKNYVVLR 537



 Score = 73.2 bits (178), Expect = 4e-10
 Identities = 63/170 (37%), Positives = 78/170 (45%), Gaps = 8/170 (4%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK KD   PKEE  D++                          +GDD K  + AP 
Sbjct: 383 PVLAPKLKD---PKEEAADSSP--VKPVPGGSSRATPSTKSAKGKRKNGDDTKPTSGAPQ 437

Query: 708 KT-VKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPAD--QSTGPVTEEEIRAVL 538
           K  VK  T                N  +P    +S  A  P     + GPV EEEIRAVL
Sbjct: 438 KKDVKSTTKDNASK----------NVAVPKAMPSSLKASTPVQGTPAQGPVAEEEIRAVL 487

Query: 537 VQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLR 403
           +Q   +T   LVA  + RLK+ EDK AFA IL +IS++QK     +VVLR
Sbjct: 488 LQRTQITTNDLVAVFKARLKTSEDKRAFAEILKKISRIQKCDNKNYVVLR 537


>ref|XP_010665736.1| PREDICTED: transcription initiation factor IIF subunit alpha isoform
            X1 [Beta vulgaris subsp. vulgaris]
            gi|870843429|gb|KMS96600.1| hypothetical protein
            BVRB_8g201300 [Beta vulgaris subsp. vulgaris]
          Length = 544

 Score =  105 bits (262), Expect = 8e-20
 Identities = 44/58 (75%), Positives = 50/58 (86%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MSFDL LKPSCGGCG T+DLYGSNCKH+TLC +CGK M ENRAKCY+CG  +TRL R+
Sbjct: 1    MSFDLTLKPSCGGCGVTTDLYGSNCKHLTLCSSCGKTMAENRAKCYECGATLTRLIRE 58



 Score = 81.3 bits (199), Expect = 2e-12
 Identities = 63/167 (37%), Positives = 78/167 (46%), Gaps = 5/167 (2%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK KD   PKEE  D++                          +GDD K  + AP 
Sbjct: 383 PVLAPKLKD---PKEEAADSSP--VKPVPGGSSRATPSTKSAKGKRKNGDDTKPTSGAPQ 437

Query: 708 KTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQG 529
           K VK E D                  +P +  AS   +    Q  GPV EEEIRAVL+Q 
Sbjct: 438 KKVKTENDVKSTTKDNASKNVAVPKAMPSSLKASTPVQGTPAQ--GPVAEEEIRAVLLQR 495

Query: 528 APLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLR 403
             +T   LVA  + RLK+ EDK AFA IL +IS++QK     +VVLR
Sbjct: 496 TQITTNDLVAVFKARLKTSEDKRAFAEILKKISRIQKCDNKNYVVLR 542



 Score = 80.5 bits (197), Expect = 3e-12
 Identities = 41/75 (54%), Positives = 52/75 (69%)
 Frame = -1

Query: 372 VSASTKAPADPSTGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRI 193
           + AST     P+ GP+ EEEIRAVLL+   +TT  LVA F  RLK+ E+K AF  IL++I
Sbjct: 468 LKASTPVQGTPAQGPVAEEEIRAVLLQRTQITTNDLVAVFKARLKTSEDKRAFAEILKKI 527

Query: 192 SKIRKSDEANYVVLR 148
           S+I+K D  NYVVLR
Sbjct: 528 SRIQKCDNKNYVVLR 542


>ref|XP_007154011.1| hypothetical protein PHAVU_003G083500g [Phaseolus vulgaris]
            gi|561027365|gb|ESW26005.1| hypothetical protein
            PHAVU_003G083500g [Phaseolus vulgaris]
          Length = 540

 Score =  105 bits (262), Expect = 8e-20
 Identities = 44/58 (75%), Positives = 50/58 (86%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MS DL LKP+CGGCGST+DLYGSNCKHMTLCL CGK M EN+AKC+DCG  +TRL R+
Sbjct: 1    MSIDLLLKPTCGGCGSTTDLYGSNCKHMTLCLTCGKTMAENKAKCFDCGATVTRLIRE 58



 Score = 83.2 bits (204), Expect = 4e-13
 Identities = 63/172 (36%), Positives = 83/172 (48%), Gaps = 8/172 (4%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PV+A K KD   PKEE  D +                            ++ K AN AP 
Sbjct: 374 PVIASKPKDA--PKEEPADNSPSKPAVTGTARGTLSTSKPSKGKRKL-NEETKAANGAPP 430

Query: 708 KTVKMETDXXXXXXXXXXXXXXGNNV---IPLNRVASWCAKAPADQSTGPVTEEEIRAVL 538
           K VK+E +               N     +P    +   + +P   ++GPV+EEEIRAVL
Sbjct: 431 KKVKIENEPKSTVKDENGSTSKNNTPSKGVPPTPSSKGGSSSPI--ASGPVSEEEIRAVL 488

Query: 537 VQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLRDK 397
           +Q  PLT Q LVA+ R RL+  EDK AFA IL RISK+QK     +V+LRD+
Sbjct: 489 MQKTPLTTQDLVAKFRARLRCPEDKKAFADILKRISKIQKTNASSYVILRDR 540



 Score = 79.7 bits (195), Expect = 5e-12
 Identities = 44/88 (50%), Positives = 63/88 (71%), Gaps = 3/88 (3%)
 Frame = -1

Query: 399 KNAVLSRG--PVSASTKAPADP-STGPITEEEIRAVLLESALLTTQALVAEFWERLKSKE 229
           KN   S+G  P  +S    + P ++GP++EEEIRAVL++   LTTQ LVA+F  RL+  E
Sbjct: 452 KNNTPSKGVPPTPSSKGGSSSPIASGPVSEEEIRAVLMQKTPLTTQDLVAKFRARLRCPE 511

Query: 228 EKNAFTAILRRISKIRKSDEANYVVLRD 145
           +K AF  IL+RISKI+K++ ++YV+LRD
Sbjct: 512 DKKAFADILKRISKIQKTNASSYVILRD 539


>ref|XP_004293352.1| PREDICTED: transcription initiation factor IIF subunit alpha
            [Fragaria vesca subsp. vesca]
          Length = 550

 Score =  105 bits (262), Expect = 8e-20
 Identities = 46/58 (79%), Positives = 49/58 (84%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MS DL LKPSCGGCGSTSDLYGSNCKHMTLC  CGK M EN+AKCYDCG  +TRL R+
Sbjct: 1    MSSDLLLKPSCGGCGSTSDLYGSNCKHMTLCCACGKTMAENKAKCYDCGAVVTRLIRE 58



 Score = 86.7 bits (213), Expect = 4e-14
 Identities = 66/170 (38%), Positives = 87/170 (51%), Gaps = 8/170 (4%)
 Frame = -1

Query: 882 LAPKKKDTHTPKEELVDTT-AKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPLK 706
           LAPK+KD   PK+E VD +  K                        +GDD K  NSAP K
Sbjct: 388 LAPKQKDA--PKQEPVDNSPVKPGASGSAAKANPSASKSSKGKRKSNGDDNKTPNSAPPK 445

Query: 705 TVKMETDXXXXXXXXXXXXXXGNNVIPLNRVA--SWCAKAPADQSTGPVTEEEIRAVLVQ 532
            VK E +               ++ +P    A  S     P+   TGPVTEEEIRAVL+Q
Sbjct: 446 KVKTENEQKSVKEEPAP-----DSTVPAKGTAAPSKPGSTPSGGRTGPVTEEEIRAVLMQ 500

Query: 531 GAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLRDK 397
            +P+T + LV     RLK+KE+K+AFAAIL  +S++QK     +VVLR+K
Sbjct: 501 KSPITTRDLVNNFPGRLKTKEEKDAFAAILRIMSRIQKNNGMSYVVLREK 550



 Score = 77.0 bits (188), Expect = 3e-11
 Identities = 39/69 (56%), Positives = 55/69 (79%)
 Frame = -1

Query: 351 PADPSTGPITEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRISKIRKSD 172
           P+   TGP+TEEEIRAVL++ + +TT+ LV  F  RLK+KEEK+AF AILR +S+I+K++
Sbjct: 481 PSGGRTGPVTEEEIRAVLMQKSPITTRDLVNNFPGRLKTKEEKDAFAAILRIMSRIQKNN 540

Query: 171 EANYVVLRD 145
             +YVVLR+
Sbjct: 541 GMSYVVLRE 549


>ref|XP_014506009.1| PREDICTED: transcription initiation factor IIF subunit alpha-like
            [Vigna radiata var. radiata]
          Length = 538

 Score =  105 bits (261), Expect = 1e-19
 Identities = 44/58 (75%), Positives = 50/58 (86%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MS DL LKP+CGGCGST+DLYGSNCKHMTLCL CGK M EN+AKC+DCG  +TRL R+
Sbjct: 1    MSIDLLLKPTCGGCGSTTDLYGSNCKHMTLCLTCGKNMAENKAKCFDCGAIVTRLIRE 58



 Score = 82.8 bits (203), Expect = 6e-13
 Identities = 57/167 (34%), Positives = 89/167 (53%), Gaps = 10/167 (5%)
 Frame = -1

Query: 615 VASWCAKAPADQSTGPVTEEEIRAVLVQGAPLTPQALVAESRERLKSKEDKNAFAAILSR 436
           +AS    AP ++ T     + +     QG   T +     S+ + K  E+  A      +
Sbjct: 375 IASKQKDAPKEEPTDNSPSKPVVTGTAQGTLSTSKP----SKGKRKFNEETKATTGAPPK 430

Query: 435 ISKVQKFV--VLRDKNAVLSRGPVSASTKAPADPS--------TGPITEEEIRAVLLESA 286
             K++     +++D+N   S+    A    PA  S        +GP++EEEIRAVL++  
Sbjct: 431 KVKIENEPKSIVKDENGSASKNNTPAKGVPPAPSSKGGSSPIPSGPVSEEEIRAVLMQKT 490

Query: 285 LLTTQALVAEFWERLKSKEEKNAFTAILRRISKIRKSDEANYVVLRD 145
            LTTQ LVA+F  RL+  E+K AF  IL+RISKI+K++ ++YV+LRD
Sbjct: 491 PLTTQDLVAKFRARLRCPEDKKAFADILKRISKIQKTNTSSYVILRD 537


>ref|XP_011008636.1| PREDICTED: transcription initiation factor IIF subunit alpha [Populus
            euphratica]
          Length = 539

 Score =  105 bits (261), Expect = 1e-19
 Identities = 44/58 (75%), Positives = 49/58 (84%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MSFDL L+PSC GCGST+DLYGSNCKHMTLCL CGK M ENR KC+DCG  +TRL R+
Sbjct: 1    MSFDLQLQPSCSGCGSTTDLYGSNCKHMTLCLTCGKAMAENRGKCFDCGAIVTRLIRE 58



 Score =  101 bits (252), Expect = 1e-18
 Identities = 69/169 (40%), Positives = 83/169 (49%), Gaps = 5/169 (2%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK+KD   PKEE  D +                          + DDAK +N AP+
Sbjct: 373 PVLAPKQKDA-VPKEEPADNSPAKPTPPGSAKGTPSTSKSAKGKRKLNADDAKTSNGAPV 431

Query: 708 KTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQG 529
           K VK E +                       + S     P   +TGPVTEEEIRAVL+Q 
Sbjct: 432 KKVKTENE--VKPTVKEESSPATKGTATPKAMPSSSKTGPTSGATGPVTEEEIRAVLLQN 489

Query: 528 APLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLRDK 397
            P+T Q LVA  + RL++ EDK AFA IL RISK+QK     FVVLRDK
Sbjct: 490 GPVTTQDLVARFKSRLRTPEDKKAFADILRRISKIQKTSGSNFVVLRDK 538



 Score = 89.4 bits (220), Expect = 6e-15
 Identities = 57/156 (36%), Positives = 91/156 (58%), Gaps = 7/156 (4%)
 Frame = -1

Query: 591 PADQSTGPVTEEEIRAVLVQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQKFV 412
           PAD S    T         +G P T ++  A+ + +L + + K +  A + ++    +  
Sbjct: 388 PADNSPAKPTPPGS----AKGTPSTSKS--AKGKRKLNADDAKTSNGAPVKKVKTENEVK 441

Query: 411 -VLRDKNAVLSRGPV------SASTKAPADPSTGPITEEEIRAVLLESALLTTQALVAEF 253
             ++++++  ++G        S+S   P   +TGP+TEEEIRAVLL++  +TTQ LVA F
Sbjct: 442 PTVKEESSPATKGTATPKAMPSSSKTGPTSGATGPVTEEEIRAVLLQNGPVTTQDLVARF 501

Query: 252 WERLKSKEEKNAFTAILRRISKIRKSDEANYVVLRD 145
             RL++ E+K AF  ILRRISKI+K+  +N+VVLRD
Sbjct: 502 KSRLRTPEDKKAFADILRRISKIQKTSGSNFVVLRD 537


>ref|XP_010270094.1| PREDICTED: transcription initiation factor IIF subunit alpha-like
            [Nelumbo nucifera] gi|720045081|ref|XP_010270096.1|
            PREDICTED: transcription initiation factor IIF subunit
            alpha-like [Nelumbo nucifera]
          Length = 540

 Score =  105 bits (261), Expect = 1e-19
 Identities = 45/58 (77%), Positives = 49/58 (84%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MS DL LKPSCGGCGSTSDLYGSNCKHMTLCL+CGK M EN  KCY+CG  +TRL R+
Sbjct: 1    MSSDLLLKPSCGGCGSTSDLYGSNCKHMTLCLSCGKTMAENHGKCYECGVPVTRLIRE 58



 Score =  103 bits (257), Expect = 3e-19
 Identities = 73/169 (43%), Positives = 87/169 (51%), Gaps = 5/169 (2%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK+KD  +PKEE  D++                          +GDD KV N AP 
Sbjct: 378 PVLAPKQKD--SPKEEPADSSPSKPVPSGSARGTPSTSKSSKSKRKSAGDDGKVPNGAPA 435

Query: 708 KTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQG 529
           K  K E +                NV P    +      P    TGPVTE+EIRAVL+Q 
Sbjct: 436 KKGKFENE-SKSSVKDEPTSTSRGNVPPKGSASLTTGSTP---PTGPVTEDEIRAVLLQM 491

Query: 528 APLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLRDK 397
           AP+T Q LVA+ + RLKSKEDK AFA IL RISK+QK     +VVLRDK
Sbjct: 492 APVTTQDLVAKFKARLKSKEDKAAFAEILRRISKIQKTNGSNYVVLRDK 540



 Score = 96.3 bits (238), Expect = 5e-17
 Identities = 68/154 (44%), Positives = 88/154 (57%), Gaps = 5/154 (3%)
 Frame = -1

Query: 591 PADQSTG-PVTEEEIRAVLVQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQKF 415
           PAD S   PV     R     G P T ++  ++ +      +  N   A   +     K 
Sbjct: 392 PADSSPSKPVPSGSAR-----GTPSTSKSSKSKRKSAGDDGKVPNGAPAKKGKFENESKS 446

Query: 414 VVLRDKNAVLSRGPV----SASTKAPADPSTGPITEEEIRAVLLESALLTTQALVAEFWE 247
            V +D+    SRG V    SAS    + P TGP+TE+EIRAVLL+ A +TTQ LVA+F  
Sbjct: 447 SV-KDEPTSTSRGNVPPKGSASLTTGSTPPTGPVTEDEIRAVLLQMAPVTTQDLVAKFKA 505

Query: 246 RLKSKEEKNAFTAILRRISKIRKSDEANYVVLRD 145
           RLKSKE+K AF  ILRRISKI+K++ +NYVVLRD
Sbjct: 506 RLKSKEDKAAFAEILRRISKIQKTNGSNYVVLRD 539


>ref|XP_012080961.1| PREDICTED: transcription initiation factor IIF subunit alpha-like
            [Jatropha curcas] gi|643719802|gb|KDP30477.1|
            hypothetical protein JCGZ_16156 [Jatropha curcas]
          Length = 530

 Score =  105 bits (261), Expect = 1e-19
 Identities = 46/58 (79%), Positives = 48/58 (82%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MSFDL LKPSC GCGST+DLYGSNCKHMTLCL CGK M EN  KCYDCG  ITRL R+
Sbjct: 1    MSFDLLLKPSCSGCGSTTDLYGSNCKHMTLCLTCGKTMAENLEKCYDCGVTITRLIRE 58



 Score = 92.8 bits (229), Expect = 5e-16
 Identities = 64/166 (38%), Positives = 82/166 (49%), Gaps = 5/166 (3%)
 Frame = -1

Query: 885 VLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPLK 706
           VLAPKKKDT  PKEE  ++                           +GDDAK +NS P K
Sbjct: 369 VLAPKKKDT--PKEEPTESNHAKSMPSGSAKGNQSASKSAKGKRKSNGDDAKTSNSVPQK 426

Query: 705 TVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQGA 526
            VK E +               N     ++V     K  +  S GPV+EEEIRAVL Q  
Sbjct: 427 KVKAENELKHTVKEESVAATKSNVT---SKVVPSSLKTESTSSIGPVSEEEIRAVLSQNG 483

Query: 525 PLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLR 403
           P+T Q LV+  + +LK ++DK AFAA L RIS++QK     +VVLR
Sbjct: 484 PVTTQELVSRFKAKLKCRQDKEAFAATLKRISRIQKTNESSYVVLR 529



 Score = 77.4 bits (189), Expect = 2e-11
 Identities = 43/93 (46%), Positives = 66/93 (70%)
 Frame = -1

Query: 426 VQKFVVLRDKNAVLSRGPVSASTKAPADPSTGPITEEEIRAVLLESALLTTQALVAEFWE 247
           V++  V   K+ V S+  V +S K  +  S GP++EEEIRAVL ++  +TTQ LV+ F  
Sbjct: 438 VKEESVAATKSNVTSK-VVPSSLKTESTSSIGPVSEEEIRAVLSQNGPVTTQELVSRFKA 496

Query: 246 RLKSKEEKNAFTAILRRISKIRKSDEANYVVLR 148
           +LK +++K AF A L+RIS+I+K++E++YVVLR
Sbjct: 497 KLKCRQDKEAFAATLKRISRIQKTNESSYVVLR 529


>ref|XP_002308775.1| transcription initiation factor IIF alpha subunit family protein
            [Populus trichocarpa] gi|222854751|gb|EEE92298.1|
            transcription initiation factor IIF alpha subunit family
            protein [Populus trichocarpa]
          Length = 541

 Score =  105 bits (261), Expect = 1e-19
 Identities = 44/58 (75%), Positives = 49/58 (84%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MSFDL L+PSC GCGST+DLYGSNCKHMTLCL CGK M ENR KC+DCG  +TRL R+
Sbjct: 1    MSFDLQLQPSCSGCGSTTDLYGSNCKHMTLCLTCGKTMAENRGKCFDCGAIVTRLIRE 58



 Score =  100 bits (250), Expect = 2e-18
 Identities = 70/169 (41%), Positives = 84/169 (49%), Gaps = 5/169 (2%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK+KD   PKEE  D +                          + DDAK +N AP+
Sbjct: 375 PVLAPKQKDA-VPKEEPADNSPAKPTPPGSAKGTPSTSKSAKGKRKLNADDAKTSNGAPV 433

Query: 708 KTVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRAVLVQG 529
           K VK E +              G     +  + S     P    TGPVTEEEIRAVL+Q 
Sbjct: 434 KKVKTENEVKPTVKEESSPATKGTATPKI--MPSSSKTGPTSGPTGPVTEEEIRAVLLQN 491

Query: 528 APLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLRDK 397
            P+T Q LVA  + RL++ EDK AFA IL RISK+QK     FVVLRDK
Sbjct: 492 GPVTTQDLVARFKSRLRTPEDKKAFADILRRISKIQKTSGSNFVVLRDK 540



 Score = 88.6 bits (218), Expect = 1e-14
 Identities = 57/156 (36%), Positives = 90/156 (57%), Gaps = 7/156 (4%)
 Frame = -1

Query: 591 PADQSTGPVTEEEIRAVLVQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQKFV 412
           PAD S    T         +G P T ++  A+ + +L + + K +  A + ++    +  
Sbjct: 390 PADNSPAKPTPPGS----AKGTPSTSKS--AKGKRKLNADDAKTSNGAPVKKVKTENEVK 443

Query: 411 -VLRDKNAVLSRGPV------SASTKAPADPSTGPITEEEIRAVLLESALLTTQALVAEF 253
             ++++++  ++G        S+S   P    TGP+TEEEIRAVLL++  +TTQ LVA F
Sbjct: 444 PTVKEESSPATKGTATPKIMPSSSKTGPTSGPTGPVTEEEIRAVLLQNGPVTTQDLVARF 503

Query: 252 WERLKSKEEKNAFTAILRRISKIRKSDEANYVVLRD 145
             RL++ E+K AF  ILRRISKI+K+  +N+VVLRD
Sbjct: 504 KSRLRTPEDKKAFADILRRISKIQKTSGSNFVVLRD 539


>ref|XP_006380878.1| hypothetical protein POPTR_0006s01070g [Populus trichocarpa]
            gi|550335193|gb|ERP58675.1| hypothetical protein
            POPTR_0006s01070g [Populus trichocarpa]
          Length = 536

 Score =  105 bits (261), Expect = 1e-19
 Identities = 44/58 (75%), Positives = 49/58 (84%)
 Frame = -1

Query: 1215 MSFDLNLKPSCGGCGSTSDLYGSNCKHMTLCLNCGKRMTENRAKCYDCGTQITRLTRD 1042
            MSFDL L+PSC GCGST+DLYGSNCKHMTLCL CGK M ENR KC+DCG  +TRL R+
Sbjct: 1    MSFDLQLQPSCSGCGSTTDLYGSNCKHMTLCLTCGKTMAENRGKCFDCGAIVTRLIRE 58



 Score = 96.3 bits (238), Expect = 5e-17
 Identities = 71/174 (40%), Positives = 85/174 (48%), Gaps = 10/174 (5%)
 Frame = -1

Query: 888 PVLAPKKKDTHTPKEELVDTTAKXXXXXXXXXXXXXXXXXXXXXXXXSGDDAKVANSAPL 709
           PVLAPK+KD   PKEE  D +                          + DDAK +N AP+
Sbjct: 375 PVLAPKQKDA-VPKEEPADNSPAKPTPPGSAKGTPSTSKSAKGKRKLNADDAKTSNGAPV 433

Query: 708 K-----TVKMETDXXXXXXXXXXXXXXGNNVIPLNRVASWCAKAPADQSTGPVTEEEIRA 544
           K     TVK E+                  ++P     S     P    TGPVTEEEIRA
Sbjct: 434 KKEVKPTVKEESSPATKGTATP-------KIMP-----SSSKTGPTSGPTGPVTEEEIRA 481

Query: 543 VLVQGAPLTPQALVAESRERLKSKEDKNAFAAILSRISKVQK-----FVVLRDK 397
           VL+Q  P+T Q LVA  + RL++ EDK AFA IL RISK+QK     FVVLRDK
Sbjct: 482 VLLQNGPVTTQDLVARFKSRLRTPEDKKAFADILRRISKIQKTSGSNFVVLRDK 535



 Score = 87.8 bits (216), Expect = 2e-14
 Identities = 54/120 (45%), Positives = 76/120 (63%), Gaps = 8/120 (6%)
 Frame = -1

Query: 480 KSKEDKNAFAAILSRISKVQKFV--VLRDKNAVLSRGPV------SASTKAPADPSTGPI 325
           K K   NA  A  S  + V+K V   ++++++  ++G        S+S   P    TGP+
Sbjct: 415 KGKRKLNADDAKTSNGAPVKKEVKPTVKEESSPATKGTATPKIMPSSSKTGPTSGPTGPV 474

Query: 324 TEEEIRAVLLESALLTTQALVAEFWERLKSKEEKNAFTAILRRISKIRKSDEANYVVLRD 145
           TEEEIRAVLL++  +TTQ LVA F  RL++ E+K AF  ILRRISKI+K+  +N+VVLRD
Sbjct: 475 TEEEIRAVLLQNGPVTTQDLVARFKSRLRTPEDKKAFADILRRISKIQKTSGSNFVVLRD 534