BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver29_contig00001145
         (1812 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-...   832   0.0  
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   801   0.0  
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-...   800   0.0  
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   798   0.0  
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ...   726   0.0  
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   723   0.0  
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   721   0.0  
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   685   0.0  
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   676   0.0  
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum...   658   0.0  
ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   450   e-123
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   449   e-123
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   446   e-122
ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   442   e-121
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              442   e-121
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   433   e-118
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]   433   e-118
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   432   e-118
ref|XP_011030061.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   428   e-117
ref|XP_009610421.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   427   e-116

>sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
            AltName: Full=Cytochrome P450 80BX
            gi|388571234|gb|AFK73713.1| cytochrome P450 [Papaver
            somniferum]
          Length = 440

 Score =  832 bits (2149), Expect = 0.0
 Identities = 420/434 (96%), Positives = 420/434 (96%)
 Frame = -2

Query: 1712 MEIVTVALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETY 1533
            MEIVTVALIAIVFTTFLYLIVRESS              GNLLQLGEKPHSQFAQLAETY
Sbjct: 1    MEIVTVALIAIVFTTFLYLIVRESSPNGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETY 60

Query: 1532 GDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNET 1353
            GDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNET
Sbjct: 61   GDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNET 120

Query: 1352 WKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGN 1173
            WKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGN
Sbjct: 121  WKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGN 180

Query: 1172 LIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ 993
            LIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ
Sbjct: 181  LIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ 240

Query: 992  GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAST 813
            GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAST
Sbjct: 241  GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAST 300

Query: 812  IEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLI 633
            IEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLI
Sbjct: 301  IEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLI 360

Query: 632  PRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFEL 453
            PRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFEL
Sbjct: 361  PRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFEL 420

Query: 452  IPFGAGRRICPGVP 411
            IPFGAGRRICPGVP
Sbjct: 421  IPFGAGRRICPGVP 434


>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  801 bits (2070), Expect = 0.0
 Identities = 394/485 (81%), Positives = 436/485 (89%)
 Frame = -2

Query: 1703 VTVALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETYGDL 1524
            VTV+L+A+V TTFLYLI R+SS              GNLLQLGEKPHSQFA+LA+TYGDL
Sbjct: 3    VTVSLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDL 62

Query: 1523 FTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNETWKK 1344
            FTLKLG++TVVVASTPLAASE+LK HDR L GRYVFQSFRVK HVENSIVW+ECNETWKK
Sbjct: 63   FTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKK 122

Query: 1343 LRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLIF 1164
            LRKVCR +LFTQKMIE+QAE+RE K MEMV++LK+N+G EVKIVEV+FGTLVN+FGNLIF
Sbjct: 123  LRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIF 182

Query: 1163 SQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQGVY 984
            SQ+IFKLGDESSGS+EMK+H+WRMLELGNS NP DY P LG  DLFGQRKDVADCLQG+Y
Sbjct: 183  SQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIY 242

Query: 983  GVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTASTIEW 804
             VWGAMLKER+IAK   N   KNDFV++LLDSGLDDQQIN+LL+++F AGTET+ASTIEW
Sbjct: 243  SVWGAMLKERKIAKLH-NNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 301

Query: 803  ALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLIPRR 624
            AL+ELTKNP+VTA++RSELLSVVGKR VKESDI N+PYLQAFVKETLRLHP TPLL+PRR
Sbjct: 302  ALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRR 361

Query: 623  ALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFELIPF 444
            ALETC+VLNYTIPKECQIMVNAWGIGRDPK WTDPLKF+P+RFLNSSIDFKGNDFELIPF
Sbjct: 362  ALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFAPERFLNSSIDFKGNDFELIPF 421

Query: 443  GAGRRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEPPLYIV 264
            GAGRRICPGVPLATQFISLIV   +QNFDW LPKGMD SQLIMEEKFGLTLQKEPPLYIV
Sbjct: 422  GAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIV 481

Query: 263  LKTRD 249
             K RD
Sbjct: 482  PKNRD 486


>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
            AltName: Full=Cytochrome P450 80B1; AltName:
            Full=Cytochrome P450 80B3
            gi|6176562|gb|AAF05621.1|AF191772_1
            (S)-N-methylcoclaurine 3'-hydroxylase [Papaver
            somniferum]
          Length = 481

 Score =  800 bits (2066), Expect = 0.0
 Identities = 396/482 (82%), Positives = 432/482 (89%)
 Frame = -2

Query: 1694 ALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETYGDLFTL 1515
            +L+A+V TTFLYLI R+SS              GNLLQLGEKPHSQFAQLAETYGDLF+L
Sbjct: 1    SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSL 60

Query: 1514 KLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNETWKKLRK 1335
            KLG++TVVVASTPLAASE+LK HDR L GRYVFQSFRVK HVENSIVW+ECNETWKKLRK
Sbjct: 61   KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 120

Query: 1334 VCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLIFSQD 1155
            VCRT+LFTQKMIE+QAEVRE K MEMV+YLKKN G EVKI EV+FGTLVN+FGNLIFSQ+
Sbjct: 121  VCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 180

Query: 1154 IFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQGVYGVW 975
            IFKLGDESSGS+EMK+H+WRMLELGNS NP DY P LG  DLFGQ KDVADCLQG+Y VW
Sbjct: 181  IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240

Query: 974  GAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTASTIEWALT 795
            GAMLKER+IAK Q N   KNDFV++LLDSGLDDQQIN+LL+++F AGTET+ASTIEWAL+
Sbjct: 241  GAMLKERKIAK-QHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 299

Query: 794  ELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLIPRRALE 615
            ELTKNPQVTA++R ELLSVVGKR VKESDI N+PYLQAFVKETLRLHP TPLL+PRRALE
Sbjct: 300  ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 359

Query: 614  TCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFELIPFGAG 435
            TC+VLNYTIPKECQIMVNAWGIGRDPK WTDPLKFSP+RFLNSSIDFKGNDFELIPFGAG
Sbjct: 360  TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAG 419

Query: 434  RRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEPPLYIVLKT 255
            RRICPGVPLATQFISLIV + +QNFDW  PKGMD SQLIMEEKFGLTLQKEPPLYIV KT
Sbjct: 420  RRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKT 479

Query: 254  RD 249
            RD
Sbjct: 480  RD 481


>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  798 bits (2062), Expect = 0.0
 Identities = 396/482 (82%), Positives = 431/482 (89%)
 Frame = -2

Query: 1694 ALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETYGDLFTL 1515
            +L+A+V TTFLYLI R+SS              GNLLQLGEKPHSQFAQLAETYGDLF+L
Sbjct: 1    SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSL 60

Query: 1514 KLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNETWKKLRK 1335
            KLG++TVVVASTPLAASE+LK HDR L GRYVFQSFRVK HVENSIVW+ECNETWKKLRK
Sbjct: 61   KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 120

Query: 1334 VCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLIFSQD 1155
            VCRT LFTQKMIE+QAEVRE K MEMV+YLKKN G EVKI EV+FGTLVN+FGNLIFSQ+
Sbjct: 121  VCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 180

Query: 1154 IFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQGVYGVW 975
            IFKLGDESSGS+EMK+H+WRMLELGNS NP DY P LG  DLFGQ KDVADCLQG+Y VW
Sbjct: 181  IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240

Query: 974  GAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTASTIEWALT 795
            GAMLKE +IAK Q N   KNDFV++LLDSGLDDQQIN+LL+++F AGTET+ASTIEWAL+
Sbjct: 241  GAMLKESKIAK-QHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 299

Query: 794  ELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLIPRRALE 615
            ELTKNPQVTA++R ELLSVVGKR VKESDI N+PYLQAFVKETLRLHP TPLL+PRRALE
Sbjct: 300  ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 359

Query: 614  TCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFELIPFGAG 435
            TC+VLNYTIPKECQIMVNAWGIGRDPK WTDPLKFSP+RFLNSSIDFKGNDFELIPFGAG
Sbjct: 360  TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAG 419

Query: 434  RRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEPPLYIVLKT 255
            RRICPGVPLATQFISLIV + +QNFDW LPKGMD SQLIMEEKFGLTLQKEPPLYIV KT
Sbjct: 420  RRICPGVPLATQFISLIVSSLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKT 479

Query: 254  RD 249
            RD
Sbjct: 480  RD 481


>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
            californica [Expression vector pWCD2353]
          Length = 490

 Score =  726 bits (1874), Expect = 0.0
 Identities = 349/487 (71%), Positives = 414/487 (85%)
 Frame = -2

Query: 1712 MEIVTVALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETY 1533
            ME+VTVALIA++ ++ LYL+   S               GNLLQLGEKPH+QFA+LA+TY
Sbjct: 1    MEVVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTY 60

Query: 1532 GDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNET 1353
            GD+FTLK+GT+TVVVAST  AASE+LK HDR L  RYVFQSFRVK HVENSIVW++C ET
Sbjct: 61   GDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTET 120

Query: 1352 WKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGN 1173
            WK LRKVCRT+LFTQKMIE+QA VRE K  EMV+YL K +G EVKIVEVIFGTLVN+FGN
Sbjct: 121  WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 180

Query: 1172 LIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ 993
            LIFSQ+IF+LGD +SGS E K+++WRMLELGNS NP DY P+LG  DLFGQRK+VA+CL+
Sbjct: 181  LIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 240

Query: 992  GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAST 813
            G+Y +WGAML+ER++AK+     +KNDFVDV LDSGL+D QIN+LL+++F AGTET+AST
Sbjct: 241  GIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 300

Query: 812  IEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLI 633
            IEWA+TELTKNP++TA +RSEL +VVG+R+VKESD  NLPYL+A VKETLRLHPPTPLL+
Sbjct: 301  IEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLL 360

Query: 632  PRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFEL 453
            PRRALETC +LNYTIPK+CQIMVNAWGIGRDPKTW DPL FSP+RFLNSS+DF+GNDF L
Sbjct: 361  PRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSL 420

Query: 452  IPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEPPL 273
            IPFGAGRRICPG+P+A QFI+L+V  F+QN DW LP GM    LI+EEKFGLTLQKEPPL
Sbjct: 421  IPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPL 480

Query: 272  YIVLKTR 252
            +IV K+R
Sbjct: 481  FIVPKSR 487


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
            AltName: Full=Cytochrome P450 80B2
            gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
            3'-hydroxylase [Eschscholzia californica]
          Length = 488

 Score =  723 bits (1867), Expect = 0.0
 Identities = 348/487 (71%), Positives = 413/487 (84%)
 Frame = -2

Query: 1712 MEIVTVALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETY 1533
            ME+VTVALIA++ ++ LYL+   S               GNLLQLGEKPH+QFA+LA+TY
Sbjct: 1    MEVVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTY 60

Query: 1532 GDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNET 1353
            GD+FTLK+GT+TVVVAST  AASE+LK HDR L  RYVFQSFRVK HVENSIVW++C ET
Sbjct: 61   GDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTET 120

Query: 1352 WKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGN 1173
            WK LRKVCRT+LFTQKMIE+QA VRE K  EMV+YL K +G EVKIVEVIFGTLVN+FGN
Sbjct: 121  WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 180

Query: 1172 LIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ 993
            LIFSQ+IF+LG  +SGS E K+++WRMLELGNS NP DY P+LG  DLFGQRK+VA+CL+
Sbjct: 181  LIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 240

Query: 992  GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAST 813
            G+Y +WGAML+ER++AK+     +KNDFVDV LDSGL+D QIN+LL+++F AGTET+AST
Sbjct: 241  GIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 300

Query: 812  IEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLI 633
            IEWA+TELTKNP++TA +RSEL +VVG+R+VKESD  NLPYL+A VKETLRLHPPTPLL+
Sbjct: 301  IEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLL 360

Query: 632  PRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFEL 453
            PRRALETC +LNYTIPK+CQIMVNAWGIGRDPKTW DPL FSP+RFLNSS+DF+GNDF L
Sbjct: 361  PRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSL 420

Query: 452  IPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEPPL 273
            IPFGAGRRICPG+P+A QFI+L+V  F+QN DW LP GM    LI+EEKFGLTLQKEPPL
Sbjct: 421  IPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPL 480

Query: 272  YIVLKTR 252
            +IV K+R
Sbjct: 481  FIVPKSR 487


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
            AltName: Full=Cytochrome P450 80B1
            gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
            3'-hydroxylase [Eschscholzia californica]
          Length = 487

 Score =  721 bits (1860), Expect = 0.0
 Identities = 347/483 (71%), Positives = 411/483 (85%)
 Frame = -2

Query: 1700 TVALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETYGDLF 1521
            TVALIA++ ++ LYL+   S               GNLLQLGEKPH+QFA+LA+TYGD+F
Sbjct: 4    TVALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIF 63

Query: 1520 TLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNETWKKL 1341
            TLK+GT+TVVVAST  AASE+LK HDR L  RYVFQSFRVK HVENSIVW++C ETWK L
Sbjct: 64   TLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNL 123

Query: 1340 RKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLIFS 1161
            RKVCRT+LFTQKMIE+QA VRE K  EMV+YL K +G EVKIVEVIFGTLVN+FGNLIFS
Sbjct: 124  RKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFS 183

Query: 1160 QDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQGVYG 981
            Q+IF+LGD +SGS E K+++WRMLELGNS NP DY P+LG  DLFGQRK+VA+CL+G+Y 
Sbjct: 184  QNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYA 243

Query: 980  VWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTASTIEWA 801
            +WGAML+ER++AK+     +KNDFVDV LDSGL+D QIN+LL+++F AGTET+ASTIEWA
Sbjct: 244  IWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWA 303

Query: 800  LTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLIPRRA 621
            +TELTKNP++TA IRSE+ +VVG+R+VKESD  NLPYL+A VKETLRLHPPTPLL+PRRA
Sbjct: 304  MTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 363

Query: 620  LETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFELIPFG 441
            LETC +LNYTIPK+CQIMVNAWGIGRDPKTWTDPL FSP+RFLNSS+DF+GNDF LIPFG
Sbjct: 364  LETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFG 423

Query: 440  AGRRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEPPLYIVL 261
            AGRRICPG+P+A QFI+L+V  F+QN DW LP GM    LI+EEKFGLTLQKEPPL+IV 
Sbjct: 424  AGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVP 483

Query: 260  KTR 252
            K+R
Sbjct: 484  KSR 486


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2; AltName: Full=Cytochrome P450 80B2 [Coptis japonica]
            gi|9971208|dbj|BAB12433.1|
            (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  685 bits (1768), Expect = 0.0
 Identities = 325/487 (66%), Positives = 404/487 (82%)
 Frame = -2

Query: 1712 MEIVTVALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETY 1533
            ME++++A+++  F  FL+ I+R+S               GNLLQLG+KPH++FA+LA+ Y
Sbjct: 1    MEVLSIAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 60

Query: 1532 GDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNET 1353
            G+LF+LKLG+QTVVVAS+P AA+E+LK HD+ L GRYVFQSFRVK HVENSIVW+ECN+ 
Sbjct: 61   GELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120

Query: 1352 WKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGN 1173
            WK LRKVCRT+LFT KMIE+Q+E+RE K  EMVK+L+  EG  VKIVEV+FGTLVN+FGN
Sbjct: 121  WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGN 180

Query: 1172 LIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ 993
            LIFS+D+F L D + GS+E+K+H+W++L++GNS NP DY P++G LDLFGQR+ VA+ LQ
Sbjct: 181  LIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQ 240

Query: 992  GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAST 813
             +Y VWG MLKERR  K     ++KNDFVDVLL++GLDDQ+IN+LL+++F AGTET+AST
Sbjct: 241  QIYDVWGVMLKERRGTK---GSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSAST 297

Query: 812  IEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLI 633
            IEWA+TELTK P V + IR EL++VVG   VKESD+ +LPYLQAFVKETLRLHPPTPLL+
Sbjct: 298  IEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLL 357

Query: 632  PRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFEL 453
            PRRALETC V+NYTIPKECQIMVNAW IGRDPKTW DPL F P+RFL+S +D+KGNDFEL
Sbjct: 358  PRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFEL 417

Query: 452  IPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEPPL 273
            IPFG GRRICPG+PLA+QF +LIV   +QNF+W LP+GM  S+L M+EKFGLTLQK+PPL
Sbjct: 418  IPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPL 477

Query: 272  YIVLKTR 252
             IVLK R
Sbjct: 478  LIVLKAR 484


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  676 bits (1745), Expect = 0.0
 Identities = 324/487 (66%), Positives = 398/487 (81%)
 Frame = -2

Query: 1712 MEIVTVALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETY 1533
            ME+V++A+++  F  FL+ I+R+                GNLLQLG+KP ++FA+LA+ Y
Sbjct: 1    MEVVSIAIVSFSFLLFLFFILRDPRQKNLPPGPRPLPIVGNLLQLGDKPRAEFAKLAQKY 60

Query: 1532 GDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNET 1353
            G LF+LKLG+QTVVVAS+P AA+EVLK HD+ L GRYVFQSFRVK HVENSIVW+ECN+ 
Sbjct: 61   GQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120

Query: 1352 WKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGN 1173
            WK LRKVCRT+LFT KMIE+Q+E+RE K  EMVK+L+  EG  VKIVEV+FGTLVN+FGN
Sbjct: 121  WKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGN 180

Query: 1172 LIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ 993
            LIFS+D+F L D + GS+E+K+H+W++LE+GNS NP DY P++G  DLFGQR+ VAD LQ
Sbjct: 181  LIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQ 240

Query: 992  GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAST 813
             +Y VWG  LKERR  K     ++ NDFVDVLL++GL+DQ+IN+LL+++F  GTET+AST
Sbjct: 241  QIYDVWGINLKERRGMK---GSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSAST 297

Query: 812  IEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLI 633
            IEWA+TELTK P V + IR EL++VVG   VKESD+ +LPYLQAFVKETLRLHPPTPLL+
Sbjct: 298  IEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLL 357

Query: 632  PRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFEL 453
            PRRALETC V+NYTIPKECQIMVNAW IGRDPKTW DPL F P+RFL+S +D+KGNDFEL
Sbjct: 358  PRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFEL 417

Query: 452  IPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEPPL 273
            IPFG GRRICPG+PLA+QFISLIV   +QNF+W LP+GM  S+L M+EKFGLTLQK+PPL
Sbjct: 418  IPFGGGRRICPGLPLASQFISLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPL 477

Query: 272  YIVLKTR 252
             IVLK R
Sbjct: 478  LIVLKAR 484


>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
            glaucum]
          Length = 491

 Score =  658 bits (1697), Expect = 0.0
 Identities = 313/488 (64%), Positives = 398/488 (81%), Gaps = 1/488 (0%)
 Frame = -2

Query: 1712 MEIVTVALIAIVFT-TFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAET 1536
            ME+++ A++++ F   F++ I+ +S               GNLLQLGEKPH++FA+LAE 
Sbjct: 1    MEVLSAAMVSLFFIFLFIFFILCDSRNKDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEK 60

Query: 1535 YGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNE 1356
            YG+LFTLKLG+QTVVVAS+P AA+E+LK  D+ L GRYVFQSFRV  HV NSIVW+ECNE
Sbjct: 61   YGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNE 120

Query: 1355 TWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFG 1176
             WK LRKVCRT+LF+ KMIE+QA +RE K ++MV++L+K E  EVKIVEV+F TLVN+FG
Sbjct: 121  NWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFG 180

Query: 1175 NLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCL 996
            NLIFS+D+F L D + GS E+K+H+W++L++GNS NP DY P++G  DLFGQR++VA  L
Sbjct: 181  NLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVL 240

Query: 995  QGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAS 816
            + +Y VWG MLKERR     I G  +NDF +VLL++GLDDQ+IN+LL+++F AGTETTAS
Sbjct: 241  KQIYDVWGVMLKERR----SITGHRENDFANVLLNAGLDDQKINALLMELFGAGTETTAS 296

Query: 815  TIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLL 636
            TIEWA+TELT N +V + +R+EL++VVG + VKES+I +LPYLQA VKETLRLHPPTPLL
Sbjct: 297  TIEWAITELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKETLRLHPPTPLL 356

Query: 635  IPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFE 456
            +PRRALETC+V+NYTIPKECQIMVNAW IGRDPKTW DPL F P+RF+NS++D+KGNDFE
Sbjct: 357  LPRRALETCKVMNYTIPKECQIMVNAWAIGRDPKTWDDPLTFKPERFMNSTVDYKGNDFE 416

Query: 455  LIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEPP 276
            LIPFG GRRICPG+PLA+QF+SLIV   +QNFDW  P+GM  +++ M+EKFGL LQK+PP
Sbjct: 417  LIPFGGGRRICPGLPLASQFLSLIVATLVQNFDWSFPQGMTPNEVPMDEKFGLPLQKDPP 476

Query: 275  LYIVLKTR 252
            L IVLK R
Sbjct: 477  LLIVLKAR 484


>ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Populus euphratica]
          Length = 492

 Score =  450 bits (1158), Expect = e-123
 Identities = 217/450 (48%), Positives = 311/450 (69%), Gaps = 3/450 (0%)
 Frame = -2

Query: 1592 NLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQ 1413
            N+L  G+KPH   A  A+T+G L +LKLG Q ++V S+  AA+E+LK+HDR L  RYVF+
Sbjct: 45   NILHFGKKPHISTANFAKTHGPLISLKLGKQLLIVGSSKRAATEILKSHDRLLSARYVFK 104

Query: 1412 SFRVKNHVEN--SIVW-NECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLK 1242
            +   +NHV +  SIVW  +C++ WK LR +CR +LF+ K IE+QA +RE K  EMV+++ 
Sbjct: 105  AALFENHVLDRISIVWATQCSDGWKSLRALCRNELFSAKAIESQAVLREKKMGEMVEFIG 164

Query: 1241 KNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPG 1062
            + +G  V I EV+   + N   NL+FS D+  L D+ + +  +K  +WRM++LG + N  
Sbjct: 165  RRKGEVVGIGEVVLAIVFNTIANLLFSVDLIGLEDDGAAT-GLKSLMWRMMKLGATPNIS 223

Query: 1061 DYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGL 882
            D+ P+LG +D  G ++ +A C+  ++G+WG  +KERR  ++ ++   ++DF+DV L +G 
Sbjct: 224  DFYPILGGIDPQGLKRKMAVCVNQMFGIWGKYIKERR--EKHVHDGPRSDFLDVFLANGF 281

Query: 881  DDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDIL 702
            +D QIN L L++ SAGT+TTA+T+EWA+ EL KN +V   +  E+       ++KES + 
Sbjct: 282  EDLQINWLALELLSAGTDTTATTVEWAIAELLKNKEVLKKVSEEIKRETDTNSLKESHVS 341

Query: 701  NLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTD 522
             LPYL A V ETLRLHPP P LIPRRALETC+V++YTIP++ +++VN W +GRDP  W D
Sbjct: 342  QLPYLNACVNETLRLHPPVPFLIPRRALETCKVMDYTIPRDSEVIVNVWAVGRDPSLWED 401

Query: 521  PLKFSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPK 342
            PL F P+RFL S +DFKG+DFE +PFGAGRRICPG+P+A + + LI+   L  FDW LP 
Sbjct: 402  PLSFKPERFLGSDLDFKGHDFEFLPFGAGRRICPGLPMAAKQVHLIIATLLYYFDWSLPN 461

Query: 341  GMDRSQLIMEEKFGLTLQKEPPLYIVLKTR 252
            G D + L M EKFG+TLQKE PL +V + R
Sbjct: 462  GEDPATLDMSEKFGITLQKEQPLLLVPRRR 491


>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
            communis] gi|223549945|gb|EEF51432.1|
            (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
            [Ricinus communis]
          Length = 496

 Score =  449 bits (1154), Expect = e-123
 Identities = 218/488 (44%), Positives = 317/488 (64%), Gaps = 3/488 (0%)
 Frame = -2

Query: 1706 IVTVALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETYGD 1527
            ++ + ++ ++ T  +  I                   GN+LQ+ +K H   A  A+ YG 
Sbjct: 10   LLPLPILVVILTQIISFISSRRQHLPLPPGPKPWPILGNILQVDKKLHICIANFAKVYGP 69

Query: 1526 LFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVEN--SIVWNE-CNE 1356
            L +L+LG Q +VVASTP +A+EVLK HDR L  R++ ++   ++H+    +IVWN  CN+
Sbjct: 70   LISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERVAIVWNPACND 129

Query: 1355 TWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFG 1176
             WK LR +CRT+LF+ K IE+QA +RE K  EM+++L   +G  V + EV+FGT+ N   
Sbjct: 130  HWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEVVFGTIFNTIS 189

Query: 1175 NLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCL 996
            +L+FS D+    ++  G   +K  +W M+E+  S N  ++ P+L  LD  G ++ +  CL
Sbjct: 190  HLLFSTDLIGFENQIGG---VKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCL 246

Query: 995  QGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAS 816
            + ++GVW   +KERR      +   K DF+D+ L +G DD QIN L++++ SAGT+TT++
Sbjct: 247  KEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLSAGTDTTST 306

Query: 815  TIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLL 636
            ++EWA+ EL KN +    +R EL   + K  +KES +  LPYL A VKETLRLHPP P L
Sbjct: 307  SVEWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLRLHPPAPFL 366

Query: 635  IPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFE 456
            IPRRA E C+V+NYTIPK+ Q++VN W IGRDP  W DP  F P+RFL SS+D KG+DFE
Sbjct: 367  IPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLGSSLDVKGHDFE 426

Query: 455  LIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEPP 276
            LIPFG+GRRICPG+P+AT+ +SL++ + +  FDW LP G D +++ M EKFG+TLQ E P
Sbjct: 427  LIPFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKVDMTEKFGITLQMEHP 486

Query: 275  LYIVLKTR 252
            L I+ K +
Sbjct: 487  LLIIPKPK 494


>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Nelumbo nucifera]
          Length = 485

 Score =  446 bits (1147), Expect = e-122
 Identities = 221/447 (49%), Positives = 309/447 (69%), Gaps = 1/447 (0%)
 Frame = -2

Query: 1589 LLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQS 1410
            L +L ++PH + ++LA+ +G L  LK G + VVVAS+ +AA EVLK  DR L GR+   S
Sbjct: 42   LPKLKKQPHVELSKLAQRFGPLMLLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHS 101

Query: 1409 FRVKNHVENSIVWNECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEG 1230
             R+K ++E+S+VW +C + WK +RKV RT+LF+ KM++ QA  RE K  E++K+L + EG
Sbjct: 102  VRIKGYIEHSMVWADCTDYWKMVRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEG 161

Query: 1229 IEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVP 1050
             +V   +VIFG+++N+ G LIFS+D++   D +  +L MK  I +++ L    N  D  P
Sbjct: 162  EKVNFADVIFGSILNILGALIFSKDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYP 221

Query: 1049 LLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQ 870
            +LG  D  G RK  A C++ +   W A++K+RR    + +  +KNDF+ VLLDSG  D Q
Sbjct: 222  ILGGSDFQGLRKASAACVKRMNESWAAIVKQRR----KNDDHSKNDFLQVLLDSGFSDPQ 277

Query: 869  INSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGK-RAVKESDILNLP 693
            I+++LL+ F  G++T+ STIEWA+ EL +NP+    +R EL  V+ +   VKESD+ NLP
Sbjct: 278  IDAMLLETFGPGSDTSTSTIEWAMAELLRNPEKLVKVREELDRVIRRSNNVKESDLPNLP 337

Query: 692  YLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLK 513
            YL A VKETLRLHPP   L+P RA+ETCQ++NYTIPK CQ+MVN + IGRD KTW  PL 
Sbjct: 338  YLHACVKETLRLHPPVTFLLPHRAMETCQMMNYTIPKGCQLMVNTYAIGRDSKTWEKPLS 397

Query: 512  FSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPKGMD 333
            F P+RFLNS +D++GNDF+ IPFGAGRRICPG+ LAT+ + LI+ + L  FDW LP GM 
Sbjct: 398  FLPERFLNSELDYQGNDFQYIPFGAGRRICPGLSLATRVVRLILASLLHTFDWSLPDGMH 457

Query: 332  RSQLIMEEKFGLTLQKEPPLYIVLKTR 252
              +L M +KFGL LQK+ PL ++ K R
Sbjct: 458  PDELDMNDKFGLALQKDIPLVVIPKLR 484


>ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Vitis vinifera]
          Length = 493

 Score =  442 bits (1137), Expect = e-121
 Identities = 219/451 (48%), Positives = 301/451 (66%), Gaps = 4/451 (0%)
 Frame = -2

Query: 1592 NLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQ 1413
            NLLQLG+ PH + A LA+ +G L +L+LGTQ +VVAS+P AA EVLK HDRTL GRYV  
Sbjct: 50   NLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSS 109

Query: 1412 SFRVK----NHVENSIVWNECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYL 1245
            S  VK    NH+  +    EC   WK LR +CRT++F+ K +E+  E+RE K ME+V++L
Sbjct: 110  SLSVKDPKLNHLSLAFA-KECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFL 168

Query: 1244 KKNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANP 1065
               EG  VK+++++F T+ N+  N  FS D+     E      +K  I+R  ELG + N 
Sbjct: 169  ATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELGATPNL 223

Query: 1064 GDYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSG 885
             D+ P+L  L+L G +K   + L  +   W   LKERR  K++  G +  D ++  L+  
Sbjct: 224  SDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEAFLEIR 281

Query: 884  LDDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDI 705
             +D QIN ++L++FSAG +T+  TIEWA+T+L +NP V   +R EL  ++G+  V+ES +
Sbjct: 282  FEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHL 341

Query: 704  LNLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWT 525
             +LPYLQA VKETLRLHPP PLL+P RA+ETCQV+ YTIPK+ Q+ VN W +GRDPK W 
Sbjct: 342  PHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWD 401

Query: 524  DPLKFSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELP 345
            DPL F+P+RFL+S ++FKGNDFE IPFGAGRRICPG+ L  + + L++   +  FDW LP
Sbjct: 402  DPLSFTPERFLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLP 461

Query: 344  KGMDRSQLIMEEKFGLTLQKEPPLYIVLKTR 252
              MD +Q+ MEE   +TL+KE PL +V K R
Sbjct: 462  DNMDSAQIDMEEWLVITLRKENPLRLVPKVR 492


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  442 bits (1137), Expect = e-121
 Identities = 219/451 (48%), Positives = 301/451 (66%), Gaps = 4/451 (0%)
 Frame = -2

Query: 1592 NLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQ 1413
            NLLQLG+ PH + A LA+ +G L +L+LGTQ +VVAS+P AA EVLK HDRTL GRYV  
Sbjct: 1095 NLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSS 1154

Query: 1412 SFRVK----NHVENSIVWNECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYL 1245
            S  VK    NH+  +    EC   WK LR +CRT++F+ K +E+  E+RE K ME+V++L
Sbjct: 1155 SLSVKDPKLNHLSLAFA-KECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFL 1213

Query: 1244 KKNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANP 1065
               EG  VK+++++F T+ N+  N  FS D+     E      +K  I+R  ELG + N 
Sbjct: 1214 ATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELGATPNL 1268

Query: 1064 GDYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSG 885
             D+ P+L  L+L G +K   + L  +   W   LKERR  K++  G +  D ++  L+  
Sbjct: 1269 SDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEAFLEIR 1326

Query: 884  LDDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDI 705
             +D QIN ++L++FSAG +T+  TIEWA+T+L +NP V   +R EL  ++G+  V+ES +
Sbjct: 1327 FEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHL 1386

Query: 704  LNLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWT 525
             +LPYLQA VKETLRLHPP PLL+P RA+ETCQV+ YTIPK+ Q+ VN W +GRDPK W 
Sbjct: 1387 PHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWD 1446

Query: 524  DPLKFSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELP 345
            DPL F+P+RFL+S ++FKGNDFE IPFGAGRRICPG+ L  + + L++   +  FDW LP
Sbjct: 1447 DPLSFTPERFLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLP 1506

Query: 344  KGMDRSQLIMEEKFGLTLQKEPPLYIVLKTR 252
              MD +Q+ MEE   +TL+KE PL +V K R
Sbjct: 1507 DNMDSAQIDMEEWLVITLRKENPLRLVPKVR 1537



 Score =  435 bits (1119), Expect = e-119
 Identities = 221/457 (48%), Positives = 299/457 (65%), Gaps = 3/457 (0%)
 Frame = -2

Query: 1592 NLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQ 1413
            NLLQ+G+ PH++ A LA+ +G L +L+LGTQ +VVAS+P AA EVLK HDR L GRY+  
Sbjct: 590  NLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSS 649

Query: 1412 SFRVKNHVEN--SIVW-NECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLK 1242
            S  VKN   N  SIV+  +CN  WK LR +CR +LF+ K +E+Q E+RE K  E+V++L 
Sbjct: 650  SVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLA 709

Query: 1241 KNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPG 1062
              EG  VK+++++F T+ N+  N  FS D+    DE      +K  I +  E G + N  
Sbjct: 710  TKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLS 769

Query: 1061 DYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGL 882
            DY P+LG LD+ G  +   +  + +   W  +LKERR  +   N  +  DF++ LL+ G 
Sbjct: 770  DYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRS--NRSSHRDFLEALLEIGF 827

Query: 881  DDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDIL 702
            +D QIN ++L++FSAG ET++ T+EWA+ EL +N      +R EL  +VG+  V+ES + 
Sbjct: 828  EDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLP 887

Query: 701  NLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTD 522
             LPYLQA VKE LRLHPP PLL+P  A ETCQV+ YTIPK+ QI VN W + RDPK W D
Sbjct: 888  RLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDD 947

Query: 521  PLKFSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPK 342
            PL F P+RFL+S +DFKGNDFE IPFGAGRRICPG+ L  + + LI+  F+  F W LP 
Sbjct: 948  PLSFKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPG 1007

Query: 341  GMDRSQLIMEEKFGLTLQKEPPLYIVLKTRD*F*ILN 231
             MD +QL MEE   +TL+KE PL +V + R  F  +N
Sbjct: 1008 NMDSAQLDMEEWLVITLRKEQPLRLVPRVRKYFIFIN 1044



 Score =  417 bits (1071), Expect = e-113
 Identities = 207/448 (46%), Positives = 301/448 (67%), Gaps = 3/448 (0%)
 Frame = -2

Query: 1592 NLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQ 1413
            NLLQ+G   H + A LA+ +G L +L+LGTQ +VVAS+  AA EVLK HDRTL GRYV  
Sbjct: 50   NLLQMGGNLHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGRYVST 109

Query: 1412 SFRVKNHVENSIVW---NECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLK 1242
            +  V +   N +       CN  W+ L+ +CR +LF+ K +E++ E+RE K ME+V++L+
Sbjct: 110  TIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVMELVEFLE 169

Query: 1241 KNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPG 1062
            K EG  VK++++++ T+ N+  N  FS D           + +K  +    ELG + N  
Sbjct: 170  KKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEGRDVRGVLLKDLLNENAELG-ATNIL 228

Query: 1061 DYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGL 882
            D+ P+LG LD+ G RK + +  + +   W  +LKERR  K++I+G +  DF+D LL++G 
Sbjct: 229  DFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERR--KQRIHGSSHGDFLDALLETGF 286

Query: 881  DDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDIL 702
            +D QIN +++++F AG ET++ T+EWA+ EL KN      + +EL  ++G+  V+ES + 
Sbjct: 287  EDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQIIGESPVRESHLP 346

Query: 701  NLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTD 522
            +LPYLQA VKETLRLHP  PLL+P RA ETCQ++ YTIPK+  I VN W +GRDP TW D
Sbjct: 347  HLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWED 406

Query: 521  PLKFSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPK 342
            PL F P+RFL+S ++FKGNDFE IPFGAGRR+CPG+PLA + + +I+  F++ FDW  P 
Sbjct: 407  PLSFKPERFLDSKLEFKGNDFEYIPFGAGRRMCPGMPLAARLVPMILATFVRLFDWSTPG 466

Query: 341  GMDRSQLIMEEKFGLTLQKEPPLYIVLK 258
             MD +++ MEE+F +TL+KE PL ++ K
Sbjct: 467  DMDFAEIDMEERFVITLRKEQPLRLLKK 494


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Vitis vinifera]
          Length = 498

 Score =  433 bits (1114), Expect = e-118
 Identities = 219/450 (48%), Positives = 296/450 (65%), Gaps = 3/450 (0%)
 Frame = -2

Query: 1592 NLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQ 1413
            NLLQ+G+ PH++ A LA+ +G L +L+LGTQ +VVAS+P AA EVLK HDR L GRY+  
Sbjct: 50   NLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSS 109

Query: 1412 SFRVKNHVEN--SIVW-NECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLK 1242
            S  VKN   N  SIV+  +CN  WK LR +CR +LF+ K +E+Q E+RE K  E+V++L 
Sbjct: 110  SVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLA 169

Query: 1241 KNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPG 1062
              EG  VK+++++F T+ N+  N  FS D+    DE      +K  I +  E G + N  
Sbjct: 170  TKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLS 229

Query: 1061 DYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGL 882
            DY P+LG LD+ G  +   +  + +   W  +LKERR  +   N  +  DF++ LL+ G 
Sbjct: 230  DYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRS--NRSSHRDFLEALLEIGF 287

Query: 881  DDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDIL 702
            +D QIN ++L++FSAG ET++ T+EWA+ EL +N      +R EL  +VG+  V+ES + 
Sbjct: 288  EDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLP 347

Query: 701  NLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTD 522
             LPYLQA VKE LRLHPP PLL+P  A ETCQV+ YTIPK+ QI VN W + RDPK W D
Sbjct: 348  RLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDD 407

Query: 521  PLKFSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPK 342
            PL F P+RFL+S +DFKGNDFE IPFGAGRRICPG+ L  + + LI+  F+  F W LP 
Sbjct: 408  PLSFKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPG 467

Query: 341  GMDRSQLIMEEKFGLTLQKEPPLYIVLKTR 252
             MD +QL MEE   +TL+KE PL +V + R
Sbjct: 468  NMDSAQLDMEEWLVITLRKEQPLRLVPRVR 497


>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
          Length = 498

 Score =  433 bits (1113), Expect = e-118
 Identities = 219/450 (48%), Positives = 296/450 (65%), Gaps = 3/450 (0%)
 Frame = -2

Query: 1592 NLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQ 1413
            NLLQ+G+ PH++ A LA+ +G L +L+LGTQ +VVAS+P AA EVLK HDR L GRY+  
Sbjct: 50   NLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSX 109

Query: 1412 SFRVKNHVEN--SIVW-NECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLK 1242
            S  VKN   N  SIV+  +CN  WK LR +CR +LF+ K +E+Q E+RE K  E+V++L 
Sbjct: 110  SVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLA 169

Query: 1241 KNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPG 1062
              EG  VK+++++F T+ N+  N  FS D+    DE      +K  I +  E G + N  
Sbjct: 170  TKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLS 229

Query: 1061 DYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGL 882
            DY P+LG LD+ G  +   +  + +   W  +LKERR  +   N  +  DF++ LL+ G 
Sbjct: 230  DYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRS--NRSSHRDFLEALLEIGF 287

Query: 881  DDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDIL 702
            +D QIN ++L++FSAG ET++ T+EWA+ EL +N      +R EL  +VG+  V+ES + 
Sbjct: 288  EDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLP 347

Query: 701  NLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTD 522
             LPYLQA VKE LRLHPP PLL+P  A ETCQV+ YTIPK+ QI VN W + RDPK W D
Sbjct: 348  RLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDD 407

Query: 521  PLKFSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPK 342
            PL F P+RFL+S +DFKGNDFE IPFGAGRRICPG+ L  + + LI+  F+  F W LP 
Sbjct: 408  PLSFKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPG 467

Query: 341  GMDRSQLIMEEKFGLTLQKEPPLYIVLKTR 252
             MD +QL MEE   +TL+KE PL +V + R
Sbjct: 468  NMDSAQLDMEEWLVITLRKEQPLRLVPRVR 497


>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
            communis] gi|223547255|gb|EEF48750.1|
            (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
            [Ricinus communis]
          Length = 496

 Score =  432 bits (1111), Expect = e-118
 Identities = 216/450 (48%), Positives = 303/450 (67%), Gaps = 3/450 (0%)
 Frame = -2

Query: 1592 NLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQ 1413
            N+LQ+G+ PH   A  A+ +G L +L+LGT+ VVVAS+P AA+E+LK HDR L GRY+  
Sbjct: 49   NILQVGKNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPA 108

Query: 1412 SFRVKNHVENSI--VWN-ECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLK 1242
            +   +++V + I  VWN  C++ WK LR +CR++LF+ K IE+QA +RE K  EM+ +L 
Sbjct: 109  TTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLT 168

Query: 1241 KNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPG 1062
              +G  V I EV+F T  N   NL+FS+D+    D+ +   E+K  I  ++EL    N  
Sbjct: 169  SKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSFEDQGNAG-ELKTLISTLMELATCPNIA 227

Query: 1061 DYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGL 882
            D+ P+L  LD  G ++ + +CL+ ++GVW   +KERR  +R +    K DF+DV L +G 
Sbjct: 228  DFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERR--ERHVKDARKTDFLDVFLSNGF 285

Query: 881  DDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDIL 702
            DD QIN LLL++FSAG +TT +T+EWA+ EL K   V   +R EL + +GK  ++ES I 
Sbjct: 286  DDHQINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIRESHIP 345

Query: 701  NLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTD 522
             L YL A VKETLRLHPP P LIPRRA E C+V+NYTIPK  QI+VN W IGRDP  W D
Sbjct: 346  QLKYLNACVKETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWED 405

Query: 521  PLKFSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPK 342
            PL + P+RFL+S++DFKG++FE +PFG+GRRICPG+P+ T+ + LI+ + +  FDW L  
Sbjct: 406  PLSYRPERFLDSNLDFKGHNFEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQN 465

Query: 341  GMDRSQLIMEEKFGLTLQKEPPLYIVLKTR 252
            G D + L M +KF +TL+KE  L +V K +
Sbjct: 466  GDDPAMLDMNDKFSITLEKEQHLLVVPKRK 495


>ref|XP_011030061.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Populus euphratica]
          Length = 511

 Score =  428 bits (1100), Expect = e-117
 Identities = 220/489 (44%), Positives = 311/489 (63%), Gaps = 3/489 (0%)
 Frame = -2

Query: 1709 EIVTVALIAIVFTTFLYLIVRESSXXXXXXXXXXXXXXGNLLQLGEKPHSQFAQLAETYG 1530
            EI+ + L+ + F     +I   S               GNLL +G +PH   A++A+ +G
Sbjct: 25   EILFLLLLPLTFIILRNIIRSCSKSKYLPPGPKPWPIIGNLLHMGNQPHVSLAEIAKIHG 84

Query: 1529 DLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVEN--SIVW-NECN 1359
             L +L+LGTQ +VV S+  AA+E+LK HDR L  R+V Q    ++HV    ++VW  E  
Sbjct: 85   PLISLRLGTQLLVVGSSAKAATEILKTHDRFLSARHVPQVIPRESHVLRRVALVWCPESM 144

Query: 1358 ETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMF 1179
            +TWK LR +CRT+LF+ K IE+ A +RE K  E++ +L   EG  V I EV+F T+ N  
Sbjct: 145  DTWKLLRGLCRTELFSAKAIESNATLREKKVGELMDFLVAREGKVVSIGEVVFSTVFNTI 204

Query: 1178 GNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADC 999
             NL+FS D+  L +E   S  +K H+ +++ L  + N  D+ P+   LD  G R+ ++  
Sbjct: 205  SNLLFSNDLAGL-EEKGMSGGLKSHVRKLMLLVATPNIADFYPIFAGLDPQGLRRKLSKL 263

Query: 998  LQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTA 819
            ++  + +W   +KERR     ++   K DF+DV L  G DD QIN L  ++FSAGT+TTA
Sbjct: 264  VEETFAIWANNIKERR--NSYVHDSPKRDFLDVFLAKGFDDDQINWLAAELFSAGTDTTA 321

Query: 818  STIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPL 639
            +TIEWA+ E+ KN +V   +  EL   + K  + ESDI  LPYL A +KETLRLHPP PL
Sbjct: 322  TTIEWAVAEILKNKEVMKKVDEELEREITKNTISESDISQLPYLDACIKETLRLHPPVPL 381

Query: 638  LIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDF 459
            L+P RA ETC+V+ YT+PK+ Q++VN W I RDP TW DPL F PDRFL S++DFKG ++
Sbjct: 382  LVPHRAAETCEVMKYTVPKDSQVLVNVWAISRDPSTWDDPLSFKPDRFLGSNLDFKGGNY 441

Query: 458  ELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELPKGMDRSQLIMEEKFGLTLQKEP 279
            E +PFGAGRRICPG+PLA + + LI+ + ++ FDW LP G D ++L M++KFG+ LQKE 
Sbjct: 442  EFLPFGAGRRICPGLPLANKLVPLILASLIRCFDWSLPNGEDPAKLDMKDKFGVVLQKEQ 501

Query: 278  PLYIVLKTR 252
            PL +V K R
Sbjct: 502  PLLLVPKRR 510


>ref|XP_009610421.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Nicotiana tomentosiformis]
          Length = 489

 Score =  427 bits (1097), Expect = e-116
 Identities = 215/447 (48%), Positives = 304/447 (68%), Gaps = 4/447 (0%)
 Frame = -2

Query: 1592 NLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQ 1413
            N+LQLG+ PH    Q A+ +G L  L+LGTQ VV+AS+P +A+E+LK  DR L  R V +
Sbjct: 42   NILQLGKNPHISMTQFAKIHGPLICLRLGTQLVVIASSPDSAAEILKTQDRLLSARSVPR 101

Query: 1412 SFRVKNHV--ENSIVWN-ECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLK 1242
                K  V  ++SIVW+ +    WK LR  CRT LF+ K +E+QA++RE K  EM+++L+
Sbjct: 102  VAPYKLSVIDQHSIVWSSDLTNHWKSLRAFCRTHLFSAKAVESQADLREKKVFEMIEFLR 161

Query: 1241 KNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPG 1062
             N+G  VKI E++F T++N  GNL F++++  L  E + S  +K  I R +ELG   N  
Sbjct: 162  SNKGKTVKISEILFATILNTLGNLFFTKNLCDLDSEGNTS-GIKHVIRRFVELGAMPNIS 220

Query: 1061 DYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGL 882
            ++ P L +LDL G RK        +  +W  ++KE+R A    + +   DF+D+++DSG 
Sbjct: 221  EFYPFLDALDLQGLRKLTNIYQDQLISIWAEVVKEKRQAISCGSSNKNQDFLDIMIDSGF 280

Query: 881  DDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRA-VKESDI 705
             D QIN +L+++ +AGT+TT STIEWA+ EL +N +V   +++E+ S +G  A ++ES+I
Sbjct: 281  SDLQINIILMELIAAGTDTTTSTIEWAMAELLRNKKVMHKLQAEIRSKIGSNANIRESNI 340

Query: 704  LNLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWT 525
              LPYL A VKETLR+HPPTP L+PRRA ETC+++N+TIPK  ++ VN W IGRD KTW 
Sbjct: 341  SELPYLAACVKETLRIHPPTPFLVPRRAPETCKLMNFTIPKNSRLFVNVWAIGRDSKTWE 400

Query: 524  DPLKFSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVLAFLQNFDWELP 345
            D L F P+RFL+SS+DF+G DFE IPFGAGRRICPG+P A Q + LI+ + + +F+W +P
Sbjct: 401  DALSFRPERFLDSSVDFRGQDFEFIPFGAGRRICPGLPFARQEVHLILASLIHHFEWSVP 460

Query: 344  KGMDRSQLIMEEKFGLTLQKEPPLYIV 264
             G D  QL M EKFG+TLQKE PL +V
Sbjct: 461  NGEDPMQLDMGEKFGVTLQKEKPLLVV 487