BLASTX nr result
ID: Papaver27_contig00056702
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver27_contig00056702
(361 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002511335.1| hypothetical protein RCOM_1509330 [Ricinus c... 114 1e-23
ref|XP_004157826.1| PREDICTED: uncharacterized LOC101205472 [Cuc... 114 1e-23
ref|XP_004152567.1| PREDICTED: uncharacterized protein LOC101205... 114 1e-23
ref|XP_007158408.1| hypothetical protein PHAVU_002G150500g [Phas... 114 2e-23
ref|XP_002272555.1| PREDICTED: uncharacterized protein LOC100264... 114 2e-23
emb|CAN62303.1| hypothetical protein VITISV_023688 [Vitis vinifera] 114 2e-23
ref|XP_006483935.1| PREDICTED: ARM REPEAT PROTEIN INTERACTING WI... 113 2e-23
ref|XP_006438283.1| hypothetical protein CICLE_v10030994mg [Citr... 113 2e-23
ref|XP_006395542.1| hypothetical protein EUTSA_v10003833mg [Eutr... 113 2e-23
ref|XP_003517523.1| PREDICTED: uncharacterized protein LOC100779... 113 2e-23
ref|XP_002527921.1| conserved hypothetical protein [Ricinus comm... 113 2e-23
ref|XP_007044776.1| Armadillo repeat only 4 [Theobroma cacao] gi... 113 3e-23
gb|EYU28417.1| hypothetical protein MIMGU_mgv1a002917mg [Mimulus... 112 4e-23
ref|XP_006290754.1| hypothetical protein CARUB_v10016853mg [Caps... 112 4e-23
ref|XP_004309985.1| PREDICTED: uncharacterized protein LOC101304... 112 4e-23
ref|XP_006574629.1| PREDICTED: uncharacterized protein LOC100804... 112 5e-23
ref|XP_007225685.1| hypothetical protein PRUPE_ppa002891mg [Prun... 112 5e-23
ref|XP_003519830.1| PREDICTED: uncharacterized protein LOC100804... 112 5e-23
gb|EYU22527.1| hypothetical protein MIMGU_mgv1a003037mg [Mimulus... 112 7e-23
ref|XP_003613135.1| hypothetical protein MTR_5g033190 [Medicago ... 112 7e-23
>ref|XP_002511335.1| hypothetical protein RCOM_1509330 [Ricinus communis]
gi|223550450|gb|EEF51937.1| hypothetical protein
RCOM_1509330 [Ricinus communis]
Length = 602
Score = 114 bits (286), Expect = 1e-23
Identities = 60/106 (56%), Positives = 76/106 (71%), Gaps = 7/106 (6%)
Frame = -3
Query: 341 TSFHSLVRINKDLQGGKSNV-------GIRRSSMHNKYERENENPDVKLELKTKCSEALW 183
TS HSLV++NK+L S + G S H++ ERE + P+V+L+LK C ALW
Sbjct: 303 TSIHSLVQMNKELTYKNSRINRILSLDGSSHGSSHHRKEREMQPPEVQLKLKVSCVLALW 362
Query: 182 MLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
LS S SNS++ITETKGLLCLAKIIEKE+ +LQ+NCLMTVMEIT+
Sbjct: 363 KLSTGSVSNSKKITETKGLLCLAKIIEKEKGDLQLNCLMTVMEITA 408
>ref|XP_004157826.1| PREDICTED: uncharacterized LOC101205472 [Cucumis sativus]
Length = 615
Score = 114 bits (285), Expect = 1e-23
Identities = 61/109 (55%), Positives = 74/109 (67%), Gaps = 11/109 (10%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKSNVGIRRSSM-----------HNKYERENENPDVKLELKTKCSE 192
SFHS+V INK+L G N + SS + + E+E E+ +VKL+LK C+E
Sbjct: 315 SFHSVVEINKELAGKNLNTSLNSSSTSSHSDSSSRGGNQRKEKEVESSEVKLQLKVNCAE 374
Query: 191 ALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
ALW LSK S NSR+ITETKGLLCLAKIIE E ELQ NCLMTVME+T+
Sbjct: 375 ALWRLSKGSLMNSRKITETKGLLCLAKIIENEGGELQYNCLMTVMEVTA 423
>ref|XP_004152567.1| PREDICTED: uncharacterized protein LOC101205472 [Cucumis sativus]
Length = 642
Score = 114 bits (285), Expect = 1e-23
Identities = 61/109 (55%), Positives = 74/109 (67%), Gaps = 11/109 (10%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKSNVGIRRSSM-----------HNKYERENENPDVKLELKTKCSE 192
SFHS+V INK+L G N + SS + + E+E E+ +VKL+LK C+E
Sbjct: 342 SFHSVVEINKELAGKNLNTSLNSSSTSSHSDSSSRGGNQRKEKEVESSEVKLQLKVNCAE 401
Query: 191 ALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
ALW LSK S NSR+ITETKGLLCLAKIIE E ELQ NCLMTVME+T+
Sbjct: 402 ALWRLSKGSLMNSRKITETKGLLCLAKIIENEGGELQYNCLMTVMEVTA 450
>ref|XP_007158408.1| hypothetical protein PHAVU_002G150500g [Phaseolus vulgaris]
gi|561031823|gb|ESW30402.1| hypothetical protein
PHAVU_002G150500g [Phaseolus vulgaris]
Length = 611
Score = 114 bits (284), Expect = 2e-23
Identities = 60/113 (53%), Positives = 78/113 (69%), Gaps = 15/113 (13%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKSNVGIRRSSMH-NKY--------------ERENENPDVKLELKT 204
S HS+V+INK+L G S G S+ + N Y ERENE+P VKL+LK
Sbjct: 308 SIHSIVQINKELGKGSSRGGRHFSNSYSNSYLFMEGSSRGGNHRKERENEDPAVKLQLKV 367
Query: 203 KCSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
C+EALWML++ S NSR+ITETKG+LCLAKI++ E+ ELQ NCLMT+ME+T+
Sbjct: 368 SCAEALWMLARGSVINSRKITETKGMLCLAKIVQNEQGELQYNCLMTIMEVTA 420
>ref|XP_002272555.1| PREDICTED: uncharacterized protein LOC100264922 [Vitis vinifera]
Length = 605
Score = 114 bits (284), Expect = 2e-23
Identities = 63/113 (55%), Positives = 79/113 (69%), Gaps = 15/113 (13%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKSNV---------------GIRRSSMHNKYERENENPDVKLELKT 204
S HSLV+INK++ G S V G R HNK +RENE P+VK+ LKT
Sbjct: 303 SIHSLVQINKEV-GESSTVNAKLHLNSCSSMYGEGSGRGGRHNK-DRENEKPEVKINLKT 360
Query: 203 KCSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
C+EAL ML++ + SNS+RITETKGLLCLAK+IEKE+ +LQ NCLMT+MEIT+
Sbjct: 361 SCAEALRMLARGNVSNSKRITETKGLLCLAKLIEKEKGDLQFNCLMTIMEITA 413
>emb|CAN62303.1| hypothetical protein VITISV_023688 [Vitis vinifera]
Length = 943
Score = 114 bits (284), Expect = 2e-23
Identities = 63/113 (55%), Positives = 79/113 (69%), Gaps = 15/113 (13%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKSNV---------------GIRRSSMHNKYERENENPDVKLELKT 204
S HSLV+INK++ G S V G R HNK +RENE P+VK+ LKT
Sbjct: 641 SIHSLVQINKEV-GESSTVNAKLHLNSSSSMYGEGSGRGGRHNK-DRENEKPEVKINLKT 698
Query: 203 KCSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
C+EAL ML++ + SNS+RITETKGLLCLAK+IEKE+ +LQ NCLMT+MEIT+
Sbjct: 699 SCAEALRMLARGNVSNSKRITETKGLLCLAKLIEKEKGDLQFNCLMTIMEITA 751
>ref|XP_006483935.1| PREDICTED: ARM REPEAT PROTEIN INTERACTING WITH ABF2-like [Citrus
sinensis]
Length = 608
Score = 113 bits (283), Expect = 2e-23
Identities = 58/110 (52%), Positives = 80/110 (72%), Gaps = 12/110 (10%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKSNVGIRR------------SSMHNKYERENENPDVKLELKTKCS 195
S H++V+INK+++ S VG + S N+ +RENE P+VK++LK C+
Sbjct: 308 SIHTIVQINKEMEKN-SFVGSKNYRPYLYSEYCSSRSGQNRKDRENEKPEVKIKLKIACA 366
Query: 194 EALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
EALWML++ S +NSRRI+ETKGLLCLAK++EKE+ ELQ NCLMT+MEIT+
Sbjct: 367 EALWMLARGSVANSRRISETKGLLCLAKLVEKEQGELQFNCLMTIMEITA 416
>ref|XP_006438283.1| hypothetical protein CICLE_v10030994mg [Citrus clementina]
gi|557540479|gb|ESR51523.1| hypothetical protein
CICLE_v10030994mg [Citrus clementina]
Length = 608
Score = 113 bits (283), Expect = 2e-23
Identities = 58/110 (52%), Positives = 80/110 (72%), Gaps = 12/110 (10%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKSNVGIRR------------SSMHNKYERENENPDVKLELKTKCS 195
S H++V+INK+++ S VG + S N+ +RENE P+VK++LK C+
Sbjct: 308 SIHTIVQINKEMEKN-SFVGSKNYRPYLYSEYCSSRSGQNRKDRENEKPEVKIKLKIACA 366
Query: 194 EALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
EALWML++ S +NSRRI+ETKGLLCLAK++EKE+ ELQ NCLMT+MEIT+
Sbjct: 367 EALWMLARGSVANSRRISETKGLLCLAKLVEKEQGELQFNCLMTIMEITA 416
>ref|XP_006395542.1| hypothetical protein EUTSA_v10003833mg [Eutrema salsugineum]
gi|557092181|gb|ESQ32828.1| hypothetical protein
EUTSA_v10003833mg [Eutrema salsugineum]
Length = 617
Score = 113 bits (283), Expect = 2e-23
Identities = 64/116 (55%), Positives = 82/116 (70%), Gaps = 18/116 (15%)
Frame = -3
Query: 338 SFHSLVRINKDLQ------------GGKSNV----GIR--RSSMHNKYERENENPDVKLE 213
S HSLV++NKD++ KSNV G+ R++ + K ER+NENP+VK E
Sbjct: 308 SIHSLVQMNKDIEKDASLKIYRPYKSSKSNVYRDIGVSGSRTTGNFKKERDNENPEVKHE 367
Query: 212 LKTKCSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
LK C+EALWML++ + +NSRRITETKGLL LAKI+EKE ELQ NCLMT+MEIT+
Sbjct: 368 LKVNCAEALWMLARGNVANSRRITETKGLLSLAKIVEKEVGELQYNCLMTLMEITA 423
>ref|XP_003517523.1| PREDICTED: uncharacterized protein LOC100779659 [Glycine max]
Length = 611
Score = 113 bits (283), Expect = 2e-23
Identities = 60/112 (53%), Positives = 76/112 (67%), Gaps = 14/112 (12%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKSNVGIRRSSMHNKY--------------ERENENPDVKLELKTK 201
S HS+V+INK+L G SS N Y ER NE+P VKL+LK
Sbjct: 309 SIHSIVQINKELGKGGQGGWKFTSSYSNSYLFMEGSSRGGNHRKERGNEDPAVKLQLKVS 368
Query: 200 CSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
C+EALWML++ S +NSR+ITETKG+LCLAKI+E E+ ELQ+NCLMT+MEIT+
Sbjct: 369 CAEALWMLARGSVTNSRKITETKGMLCLAKIVEMEQGELQLNCLMTIMEITA 420
>ref|XP_002527921.1| conserved hypothetical protein [Ricinus communis]
gi|223532696|gb|EEF34478.1| conserved hypothetical
protein [Ricinus communis]
Length = 613
Score = 113 bits (283), Expect = 2e-23
Identities = 62/119 (52%), Positives = 78/119 (65%), Gaps = 21/119 (17%)
Frame = -3
Query: 338 SFHSLVRINKDLQ-----GGKSNV----------------GIRRSSMHNKYERENENPDV 222
S HS+V+INK+++ G K+N R S +N+ ERENE P+V
Sbjct: 303 SIHSIVQINKEIEKYPINGSKNNHFKPYMNSFSSFHSEGGSSRGGSNNNRKERENEKPEV 362
Query: 221 KLELKTKCSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
KL+LK C+EALWML+ S NS+RITETKGLLCLAKI+EKE ELQ NCLMT+ EIT+
Sbjct: 363 KLKLKITCAEALWMLASGSVLNSKRITETKGLLCLAKIVEKEEGELQYNCLMTIKEITA 421
>ref|XP_007044776.1| Armadillo repeat only 4 [Theobroma cacao]
gi|508708711|gb|EOY00608.1| Armadillo repeat only 4
[Theobroma cacao]
Length = 616
Score = 113 bits (282), Expect = 3e-23
Identities = 63/119 (52%), Positives = 77/119 (64%), Gaps = 21/119 (17%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKS---------------------NVGIRRSSMHNKYERENENPDV 222
S HS+V+INK+++ S G R H K ERENE P+V
Sbjct: 308 SIHSIVQINKEMEKNSSIGSNSRNYSYRPYTNSYSNLHMEGSSRGGNHRK-ERENEKPEV 366
Query: 221 KLELKTKCSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
K++LK +EALWML+K S SNSRRITETKGLLCLAK++EKE+ ELQ NCLMTVMEIT+
Sbjct: 367 KIQLKINGAEALWMLAKGSVSNSRRITETKGLLCLAKLVEKEQGELQYNCLMTVMEITA 425
>gb|EYU28417.1| hypothetical protein MIMGU_mgv1a002917mg [Mimulus guttatus]
Length = 625
Score = 112 bits (281), Expect = 4e-23
Identities = 57/98 (58%), Positives = 72/98 (73%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKSNVGIRRSSMHNKYERENENPDVKLELKTKCSEALWMLSKESTS 159
S H+LV INK+++ S+ R + K ERENENP VKL LK+ C+ ALWML+K S +
Sbjct: 332 SIHTLVLINKEMENKTSSYSKRAVTSQRK-ERENENPQVKLSLKSTCARALWMLAKGSVA 390
Query: 158 NSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
NS+RI ETKGLLCLAK++E E ELQ N LMT+MEIT+
Sbjct: 391 NSKRIAETKGLLCLAKLVETEEGELQKNSLMTIMEITT 428
>ref|XP_006290754.1| hypothetical protein CARUB_v10016853mg [Capsella rubella]
gi|482559461|gb|EOA23652.1| hypothetical protein
CARUB_v10016853mg [Capsella rubella]
Length = 614
Score = 112 bits (281), Expect = 4e-23
Identities = 63/121 (52%), Positives = 81/121 (66%), Gaps = 17/121 (14%)
Frame = -3
Query: 356 QPQRPTSFHSLVRINKDLQ------------GGKSNV-----GIRRSSMHNKYERENENP 228
Q + S HSLV++NKD++ KSNV G + + K ER+NENP
Sbjct: 300 QLSKHNSIHSLVQMNKDIEKDPSLKIPRPFKSSKSNVYREIGGSGSKTGNFKKERDNENP 359
Query: 227 DVKLELKTKCSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEIT 48
+VK ELK C+EALWML++ + +NS+RITETKGLL LAKI+EKE ELQ NCLMT+MEIT
Sbjct: 360 EVKHELKVSCAEALWMLARGNVANSKRITETKGLLTLAKIVEKEVGELQYNCLMTLMEIT 419
Query: 47 S 45
+
Sbjct: 420 A 420
>ref|XP_004309985.1| PREDICTED: uncharacterized protein LOC101304246 [Fragaria vesca
subsp. vesca]
Length = 602
Score = 112 bits (281), Expect = 4e-23
Identities = 57/111 (51%), Positives = 76/111 (68%), Gaps = 15/111 (13%)
Frame = -3
Query: 338 SFHSLVRINKDLQGGKS---------------NVGIRRSSMHNKYERENENPDVKLELKT 204
S HS++ INK+++ G + G R +++ +RENE P VKL++K
Sbjct: 304 SIHSVLEINKEMEKGSQRRSYTNSFSSSSYTYSEGSSRGGHNHRKDRENEQPLVKLQMKI 363
Query: 203 KCSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEI 51
C+EALWML++ S SNSRRITETKGLLCLAK++EKE+ ELQ NCLMT+MEI
Sbjct: 364 NCAEALWMLARGSISNSRRITETKGLLCLAKLVEKEQGELQYNCLMTIMEI 414
>ref|XP_006574629.1| PREDICTED: uncharacterized protein LOC100804310 isoform X2 [Glycine
max]
Length = 529
Score = 112 bits (280), Expect = 5e-23
Identities = 59/112 (52%), Positives = 80/112 (71%), Gaps = 14/112 (12%)
Frame = -3
Query: 338 SFHSLVRINKDL-QGGKSNVGIRRS-------------SMHNKYERENENPDVKLELKTK 201
S HS+V+INK+L +GG+ S ++++ ERENE+P VKL+LK
Sbjct: 227 SIHSIVQINKELGKGGRGGHQFSSSYSSSYLFTEWSSRGVNHRKERENEDPVVKLQLKVS 286
Query: 200 CSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
C+EALWML + S +NSR+ITETKG+LCLAKI+E E+ ELQ+NCLMT+MEIT+
Sbjct: 287 CAEALWMLVQGSVTNSRKITETKGMLCLAKIVEMEQGELQLNCLMTMMEITA 338
>ref|XP_007225685.1| hypothetical protein PRUPE_ppa002891mg [Prunus persica]
gi|462422621|gb|EMJ26884.1| hypothetical protein
PRUPE_ppa002891mg [Prunus persica]
Length = 623
Score = 112 bits (280), Expect = 5e-23
Identities = 62/127 (48%), Positives = 79/127 (62%), Gaps = 29/127 (22%)
Frame = -3
Query: 338 SFHSLVRINKDLQGG------------KSNV-----------------GIRRSSMHNKYE 246
S HSLV+INK+++ +SN G R +++ E
Sbjct: 305 SIHSLVQINKEMEKSTLAKSKPSSNSRESNFRPYSNSYSSSSYTYYAEGSNRGGHNHRKE 364
Query: 245 RENENPDVKLELKTKCSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLM 66
RENE P VKL+LK C+EALWML++ S SNSRRITETKGLLCLAK++E E+ ELQ NCLM
Sbjct: 365 RENEKPHVKLQLKVSCAEALWMLARGSVSNSRRITETKGLLCLAKLVEMEQGELQFNCLM 424
Query: 65 TVMEITS 45
T+MEIT+
Sbjct: 425 TIMEITA 431
>ref|XP_003519830.1| PREDICTED: uncharacterized protein LOC100804310 isoform X1 [Glycine
max]
Length = 619
Score = 112 bits (280), Expect = 5e-23
Identities = 59/112 (52%), Positives = 80/112 (71%), Gaps = 14/112 (12%)
Frame = -3
Query: 338 SFHSLVRINKDL-QGGKSNVGIRRS-------------SMHNKYERENENPDVKLELKTK 201
S HS+V+INK+L +GG+ S ++++ ERENE+P VKL+LK
Sbjct: 317 SIHSIVQINKELGKGGRGGHQFSSSYSSSYLFTEWSSRGVNHRKERENEDPVVKLQLKVS 376
Query: 200 CSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
C+EALWML + S +NSR+ITETKG+LCLAKI+E E+ ELQ+NCLMT+MEIT+
Sbjct: 377 CAEALWMLVQGSVTNSRKITETKGMLCLAKIVEMEQGELQLNCLMTMMEITA 428
>gb|EYU22527.1| hypothetical protein MIMGU_mgv1a003037mg [Mimulus guttatus]
Length = 613
Score = 112 bits (279), Expect = 7e-23
Identities = 58/111 (52%), Positives = 78/111 (70%), Gaps = 13/111 (11%)
Frame = -3
Query: 338 SFHSLVRINKDLQ--------GGKSNVGIRRSS-----MHNKYERENENPDVKLELKTKC 198
S HSLV+IN +L+ G SN+ I + + ++ ERE+E P++KLELK C
Sbjct: 311 SIHSLVQINMELEKKSLFKSGSGSSNLSIHYAEGSCKGLSHRKEREHEKPELKLELKISC 370
Query: 197 SEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
++ALWML+K S NS+RIT+TKGLLCLAK++E E ELQ NCLMT+MEIT+
Sbjct: 371 AKALWMLAKWSVPNSKRITDTKGLLCLAKLVEAEEGELQRNCLMTIMEITA 421
>ref|XP_003613135.1| hypothetical protein MTR_5g033190 [Medicago truncatula]
gi|355514470|gb|AES96093.1| hypothetical protein
MTR_5g033190 [Medicago truncatula]
Length = 656
Score = 112 bits (279), Expect = 7e-23
Identities = 61/120 (50%), Positives = 76/120 (63%), Gaps = 21/120 (17%)
Frame = -3
Query: 341 TSFHSLVRINKDL---QGGKSNVGIRRSSMHNKY------------------ERENENPD 225
TS HS+ ++NK+L +S G N Y ERENE+P
Sbjct: 302 TSIHSIFQVNKELGKKTTDRSGTGSGSRQFANSYSSTYYYTEGSSRGGNYRKERENEDPA 361
Query: 224 VKLELKTKCSEALWMLSKESTSNSRRITETKGLLCLAKIIEKERDELQMNCLMTVMEITS 45
VKL+LK C+EALWML+ S SNSR+ITETKG+LCLAKI+EKE+ ELQ NCLMT+MEIT+
Sbjct: 362 VKLQLKISCAEALWMLAAGSVSNSRKITETKGMLCLAKIVEKEQGELQRNCLMTIMEITA 421