BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver27_contig00036088
         (938 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002272782.1| PREDICTED: 4-coumarate--CoA ligase 1 isoform...   449   e-124
emb|CAN69130.1| hypothetical protein VITISV_039323 [Vitis vinifera]   449   e-124
gb|AFC89541.1| 4-coumarate: coenzyme A ligase 5 [Populus tomentosa]   440   e-121
gb|AAC39366.1| 4-coumarate:CoA ligase 1 [Populus trichocarpa x P...   439   e-120
gb|AAZ79469.1| 4-coumarate:coenzyme A ligase [Eucalyptus camaldu...   435   e-119
gb|ACX68559.1| 4-coumarate:CoA ligase [Eucalyptus camaldulensis]      435   e-119
gb|AAC39365.1| 4-coumarate:CoA ligase 2 [Populus trichocarpa x P...   434   e-119
ref|XP_006366277.1| PREDICTED: 4-coumarate--CoA ligase 2-like [S...   433   e-119
gb|AFP49810.1| 4-hydroxycinnamoyl-CoA ligase 3 [Coffea arabica]       433   e-119
sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; ...   432   e-119
gb|AFC89540.1| 4-coumarate: coenzyme A ligase 4 [Populus tomentosa]   432   e-119
gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus]      432   e-119
ref|XP_002324477.2| 4-coumarate-CoA ligase 2 family protein [Pop...   432   e-118
ref|XP_004235870.1| PREDICTED: 4-coumarate--CoA ligase 1-like [S...   432   e-118
gb|AGE10594.1| 4-coumarate CoA ligase [Lonicera japonica]             432   e-118
ref|XP_006381817.1| hypothetical protein POPTR_0006s18490g [Popu...   431   e-118
gb|AFG26323.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]        431   e-118
ref|XP_007014191.1| 4-coumarate:CoA ligase 2 [Theobroma cacao] g...   430   e-118
sp|O24540.1|4CL_VANPL RecName: Full=4-coumarate--CoA ligase; Sho...   430   e-118
gb|AEM63673.1| 4-hydroxycinnamoyl CoA ligase [Platycodon grandif...   430   e-118

>ref|XP_002272782.1| PREDICTED: 4-coumarate--CoA ligase 1 isoform 1 [Vitis vinifera]
          Length = 548

 Score =  449 bits (1156), Expect = e-124
 Identities = 221/251 (88%), Positives = 242/251 (96%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAKSPVVD YDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVL+M
Sbjct: 291  VLAIAKSPVVDEYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAM 350

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSGSCGTVVRNA+MKI+DP+TG+SLPR+Q GEICIRG QIMKGYLNDPE
Sbjct: 351  CLAFAKEPFEIKSGSCGTVVRNAQMKILDPDTGASLPRNQPGEICIRGHQIMKGYLNDPE 410

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT+ TIDK+GWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEAMLIAHPN++D
Sbjct: 411  ATERTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNVSD 470

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVVSMKDEAAGEVPVAF++RSNGS+ITE EIK+YIS QVVFYKRI++VFF ETIPKAPS
Sbjct: 471  AAVVSMKDEAAGEVPVAFIVRSNGSKITEDEIKQYISKQVVFYKRIYRVFFVETIPKAPS 530

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 531  GKILRKDLRAR 541


>emb|CAN69130.1| hypothetical protein VITISV_039323 [Vitis vinifera]
          Length = 548

 Score =  449 bits (1156), Expect = e-124
 Identities = 221/251 (88%), Positives = 242/251 (96%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAKSPVVD YDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVL+M
Sbjct: 291  VLAIAKSPVVDEYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAM 350

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSGSCGTVVRNA+MKI+DP+TG+SLPR+Q GEICIRG QIMKGYLNDPE
Sbjct: 351  CLAFAKEPFEIKSGSCGTVVRNAQMKILDPDTGASLPRNQPGEICIRGHQIMKGYLNDPE 410

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT+ TIDK+GWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEAMLIAHPN++D
Sbjct: 411  ATERTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNVSD 470

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVVSMKDEAAGEVPVAF++RSNGS+ITE EIK+YIS QVVFYKRI++VFF ETIPKAPS
Sbjct: 471  AAVVSMKDEAAGEVPVAFIVRSNGSKITEDEIKQYISKQVVFYKRIYRVFFVETIPKAPS 530

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 531  GKILRKDLRAR 541


>gb|AFC89541.1| 4-coumarate: coenzyme A ligase 5 [Populus tomentosa]
          Length = 545

 Score =  440 bits (1132), Expect = e-121
 Identities = 217/251 (86%), Positives = 237/251 (94%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLA+AK PVVD YDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM
Sbjct: 287  VLAVAKCPVVDKYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 346

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFA+EP E+KSG+CGTVVRNAEMKIVDP+TG SLPR+Q+GEICIRG QIMKGYLNDPE
Sbjct: 347  CLAFAREPFEIKSGACGTVVRNAEMKIVDPDTGRSLPRNQAGEICIRGSQIMKGYLNDPE 406

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT+ TID DGWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEAMLIAHP+I+D
Sbjct: 407  ATERTIDNDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPDISD 466

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
             AVV MKDEAAGEVPVAFV+R+NGS+ITE EIK+YIS QVVFYKRI +VFF E+IPKAPS
Sbjct: 467  CAVVPMKDEAAGEVPVAFVVRANGSKITEDEIKQYISKQVVFYKRISRVFFTESIPKAPS 526

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 527  GKILRKDLRAR 537


>gb|AAC39366.1| 4-coumarate:CoA ligase 1 [Populus trichocarpa x Populus deltoides]
          Length = 557

 Score =  439 bits (1128), Expect = e-120
 Identities = 216/251 (86%), Positives = 235/251 (93%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLA+AK PVVD YDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM
Sbjct: 287  VLAVAKCPVVDKYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 346

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSG+CGTVVRNAEMKIVDP+TG SLPR+QSGEICIRG QIMKGYLNDPE
Sbjct: 347  CLAFAKEPFEIKSGACGTVVRNAEMKIVDPDTGRSLPRNQSGEICIRGSQIMKGYLNDPE 406

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT+ T+D DGWLHTGDIGYID ++E+FIVDRLKELIKYKGFQVAPAELEAMLIAHP+I+D
Sbjct: 407  ATERTVDNDGWLHTGDIGYIDGDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPDISD 466

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
             AVV MKDEAAGEVP+AFV+R+NGS+ITE EIK+YIS QVVFYKRI +VFF E IPKAPS
Sbjct: 467  CAVVPMKDEAAGEVPIAFVVRANGSKITEDEIKQYISKQVVFYKRISRVFFTEAIPKAPS 526

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 527  GKILRKDLRAR 537


>gb|AAZ79469.1| 4-coumarate:coenzyme A ligase [Eucalyptus camaldulensis]
          Length = 544

 Score =  435 bits (1118), Expect = e-119
 Identities = 216/251 (86%), Positives = 235/251 (93%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAKS  VD YDLSSIRT+MSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVL+M
Sbjct: 287  VLAIAKSAEVDRYDLSSIRTIMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAM 346

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSG+CGTVVRNAEMKIVDPETG+SLPR+Q+GEICIRG QIMKGYLNDPE
Sbjct: 347  CLAFAKEPFEIKSGACGTVVRNAEMKIVDPETGASLPRNQAGEICIRGHQIMKGYLNDPE 406

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT  TIDK+GWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEAMLIAHP+I+D
Sbjct: 407  ATANTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPSISD 466

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVV MKDE A EVPVAFV++SNGS ITE EIK+YIS QVVFYKRI++VFF + IPKAPS
Sbjct: 467  AAVVPMKDEVASEVPVAFVVKSNGSVITEDEIKQYISKQVVFYKRINRVFFTDAIPKAPS 526

Query: 723  GKILRNDLRAK 755
            GKILR DLRAK
Sbjct: 527  GKILRKDLRAK 537


>gb|ACX68559.1| 4-coumarate:CoA ligase [Eucalyptus camaldulensis]
          Length = 544

 Score =  435 bits (1118), Expect = e-119
 Identities = 216/251 (86%), Positives = 235/251 (93%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAKS  VD YDLSSIRT+MSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVL+M
Sbjct: 287  VLAIAKSAEVDRYDLSSIRTIMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAM 346

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSG+CGTVVRNAEMKIVDPETG+SLPR+Q+GEICIRG QIMKGYLNDPE
Sbjct: 347  CLAFAKEPFEIKSGACGTVVRNAEMKIVDPETGASLPRNQAGEICIRGHQIMKGYLNDPE 406

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT  TIDK+GWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEAMLIAHP+I+D
Sbjct: 407  ATANTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPSISD 466

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVV MKDE A EVPVAFV++SNGS ITE EIK+YIS QVVFYKRI++VFF + IPKAPS
Sbjct: 467  AAVVPMKDEVASEVPVAFVVKSNGSVITEDEIKQYISKQVVFYKRINRVFFTDAIPKAPS 526

Query: 723  GKILRNDLRAK 755
            GKILR DLRAK
Sbjct: 527  GKILRKDLRAK 537


>gb|AAC39365.1| 4-coumarate:CoA ligase 2 [Populus trichocarpa x Populus deltoides]
          Length = 548

 Score =  434 bits (1116), Expect = e-119
 Identities = 217/250 (86%), Positives = 236/250 (94%)
 Frame = +3

Query: 6    LAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMC 185
            LAIAKSPVVD YDLSSIRTV+SGAAP+GKELEDTVRAKLPNAKLGQGYGMTEAGPV++MC
Sbjct: 288  LAIAKSPVVDQYDLSSIRTVLSGAAPLGKELEDTVRAKLPNAKLGQGYGMTEAGPVIAMC 347

Query: 186  LAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPEA 365
            LAFAKEP E+KSG+CGTVVRNAEMKIVDPETG S PR+++GEICIRG QIMKGYLNDPEA
Sbjct: 348  LAFAKEPFEIKSGACGTVVRNAEMKIVDPETGESQPRNKTGEICIRGCQIMKGYLNDPEA 407

Query: 366  TKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNINDA 545
            T+ TIDKDGWLHTGDIGYI DE+E+FIVDRLKELIKYKGFQVAPAELEAMLIAHPNI+DA
Sbjct: 408  TERTIDKDGWLHTGDIGYI-DEDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDA 466

Query: 546  AVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPSG 725
            AVV MKDEAAGEVPVAFV+RSNGS+ITE EIK+YIS QV+FYKRI +VFF E IPKAPSG
Sbjct: 467  AVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVIFYKRIGRVFFTEAIPKAPSG 526

Query: 726  KILRNDLRAK 755
            KILR DLRA+
Sbjct: 527  KILRKDLRAR 536


>ref|XP_006366277.1| PREDICTED: 4-coumarate--CoA ligase 2-like [Solanum tuberosum]
          Length = 548

 Score =  433 bits (1114), Expect = e-119
 Identities = 209/251 (83%), Positives = 237/251 (94%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAKSP+VDNYDLSS+RTVMSGAAP+GKELEDTVRAK+PNAKLGQGYGMTEAGPVL+M
Sbjct: 291  VLAIAKSPMVDNYDLSSVRTVMSGAAPLGKELEDTVRAKIPNAKLGQGYGMTEAGPVLAM 350

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSG+CGTVVRNAEMKIVDP+TG SLPR+QSGEICIRGDQIMKGYLNDP+
Sbjct: 351  CLAFAKEPFEIKSGACGTVVRNAEMKIVDPDTGKSLPRNQSGEICIRGDQIMKGYLNDPD 410

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT  TIDK+GWLHTGDIGYID+++E+FIVDRLKELIKYKGFQVAPAELEA+L+ HPNI+D
Sbjct: 411  ATTGTIDKEGWLHTGDIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISD 470

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVV MKDE AGEVPVAFV+RSNGS ITE E+K++I+ QV+FYKRI +VFF + +PK+PS
Sbjct: 471  AAVVPMKDEQAGEVPVAFVVRSNGSTITEDEVKEFITKQVIFYKRIKRVFFVDAVPKSPS 530

Query: 723  GKILRNDLRAK 755
            GKILR DLRAK
Sbjct: 531  GKILRKDLRAK 541


>gb|AFP49810.1| 4-hydroxycinnamoyl-CoA ligase 3 [Coffea arabica]
          Length = 543

 Score =  433 bits (1114), Expect = e-119
 Identities = 214/251 (85%), Positives = 237/251 (94%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VL IAKSPVVD YDLSSIR VMSGAAPMGKELEDTVRAKLP A LGQGYGMTEAGP+LSM
Sbjct: 285  VLEIAKSPVVDKYDLSSIRMVMSGAAPMGKELEDTVRAKLPKAVLGQGYGMTEAGPLLSM 344

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP +VKSG+CGTVVRNAEMKIVDPET  SLPR+Q+GEICIRGDQIMKGYLNDPE
Sbjct: 345  CLAFAKEPFDVKSGACGTVVRNAEMKIVDPETNLSLPRNQAGEICIRGDQIMKGYLNDPE 404

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT+ TIDK+GWLHTGDIGYIDD++EIFIVDRLKELIKYKGFQVAPAELEAML++HP I+D
Sbjct: 405  ATENTIDKEGWLHTGDIGYIDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLLSHPGISD 464

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVVSMKDEAAGEVPVAFV+R++GS+I+E EIK++IS+QV+FYKRIH+VFF + IPKAPS
Sbjct: 465  AAVVSMKDEAAGEVPVAFVVRASGSKISEDEIKQFISNQVIFYKRIHRVFFMDKIPKAPS 524

Query: 723  GKILRNDLRAK 755
            GKILR DLRAK
Sbjct: 525  GKILRKDLRAK 535


>sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName:
            Full=4-coumaroyl-CoA synthase 2 gi|1663724|gb|AAB18638.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 542

 Score =  432 bits (1112), Expect = e-119
 Identities = 211/251 (84%), Positives = 236/251 (94%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAKSP+VD+YDLSS+RTVMSGAAP+GKELEDTVRAK PNAKLGQGYGMTEAGPVL+M
Sbjct: 285  VLAIAKSPMVDDYDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAM 344

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSG+CGTVVRNAEMKIVDP+TG+SLPR+QSGEICIRGDQIMKGYLNDPE
Sbjct: 345  CLAFAKEPFEIKSGACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPE 404

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT  TIDK+GWL+TGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEA+L+ HPNI+D
Sbjct: 405  ATARTIDKEGWLYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISD 464

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVV MKDE AGEVPVAFV+RSNGS ITE E+K +IS QV+FYKRI +VFF + IPK+PS
Sbjct: 465  AAVVPMKDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPS 524

Query: 723  GKILRNDLRAK 755
            GKILR DLRAK
Sbjct: 525  GKILRKDLRAK 535


>gb|AFC89540.1| 4-coumarate: coenzyme A ligase 4 [Populus tomentosa]
          Length = 556

 Score =  432 bits (1112), Expect = e-119
 Identities = 216/249 (86%), Positives = 235/249 (94%)
 Frame = +3

Query: 6    LAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMC 185
            LAIAKSPVVD YDLSSIRTV+SGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPV++MC
Sbjct: 288  LAIAKSPVVDQYDLSSIRTVLSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVIAMC 347

Query: 186  LAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPEA 365
            LAFAKEP E+KSG+CGTVVRNAEMKIVDPETG S PR+++GEICIRG QIMKGYLNDPEA
Sbjct: 348  LAFAKEPFEIKSGACGTVVRNAEMKIVDPETGDSQPRNKAGEICIRGSQIMKGYLNDPEA 407

Query: 366  TKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNINDA 545
            T+ TIDKDGWLHTGDIGYI DE+E+FIVDRLKELIKYKGFQVAPAELE+MLIAHP+I+DA
Sbjct: 408  TERTIDKDGWLHTGDIGYI-DEDELFIVDRLKELIKYKGFQVAPAELESMLIAHPSISDA 466

Query: 546  AVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPSG 725
            AVV MKDEAAGEVPVAFV+RSNGS+ITE EIK+YIS QV+FYKRI +VFF E IPKAPSG
Sbjct: 467  AVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVIFYKRIGRVFFTEAIPKAPSG 526

Query: 726  KILRNDLRA 752
            KILR DLRA
Sbjct: 527  KILRKDLRA 535


>gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus]
          Length = 544

 Score =  432 bits (1111), Expect = e-119
 Identities = 213/251 (84%), Positives = 235/251 (93%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VL+IAK P +  YDLSSIR VMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM
Sbjct: 287  VLSIAKCPDLHRYDLSSIRMVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 346

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSG+CGTVVRNAEMKI+DP+T  SLPR+QSGEICIRG QIMKGYLNDPE
Sbjct: 347  CLAFAKEPYEIKSGACGTVVRNAEMKIIDPDTNESLPRNQSGEICIRGSQIMKGYLNDPE 406

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT+ TIDK+GWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEAMLI+HPN++D
Sbjct: 407  ATENTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLISHPNLSD 466

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVVSMKDEAAGEVPVAFV+RSNGS+I+E +IK+YIS QVVFYKRI KVFF + IPKAPS
Sbjct: 467  AAVVSMKDEAAGEVPVAFVVRSNGSKISEDDIKQYISKQVVFYKRISKVFFTDKIPKAPS 526

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 527  GKILRKDLRAR 537


>ref|XP_002324477.2| 4-coumarate-CoA ligase 2 family protein [Populus trichocarpa]
            gi|550318444|gb|EEF03042.2| 4-coumarate-CoA ligase 2
            family protein [Populus trichocarpa]
          Length = 556

 Score =  432 bits (1110), Expect = e-118
 Identities = 216/250 (86%), Positives = 235/250 (94%)
 Frame = +3

Query: 6    LAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMC 185
            LAIAKSPVVD YDLSSIRTV+SGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPV++MC
Sbjct: 288  LAIAKSPVVDQYDLSSIRTVLSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVIAMC 347

Query: 186  LAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPEA 365
            LAFAKEP E+KSG+CGTVVRNAEMKIVDPETG S PR+++GEICIRG QIMKGYLND EA
Sbjct: 348  LAFAKEPFEIKSGACGTVVRNAEMKIVDPETGDSQPRNKAGEICIRGCQIMKGYLNDTEA 407

Query: 366  TKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNINDA 545
            T+ TIDKDGWLHTGD+GYI DE+E+FIVDRLKELIKYKGFQVAPAELEAMLIAHPNI+DA
Sbjct: 408  TERTIDKDGWLHTGDVGYI-DEDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDA 466

Query: 546  AVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPSG 725
            AVV MKDEAAGEVPVAFV+RSNGS+ITE EIK+YIS QV+FYKRI +VFF E IPKAPSG
Sbjct: 467  AVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVIFYKRIGRVFFTEAIPKAPSG 526

Query: 726  KILRNDLRAK 755
            KILR DLRA+
Sbjct: 527  KILRKDLRAR 536


>ref|XP_004235870.1| PREDICTED: 4-coumarate--CoA ligase 1-like [Solanum lycopersicum]
          Length = 545

 Score =  432 bits (1110), Expect = e-118
 Identities = 209/251 (83%), Positives = 234/251 (93%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAKSP+VDNYDLSS+RTVMSGAAP+GKELED VRAK PNAKLGQGYGMTEAGPVL+M
Sbjct: 288  VLAIAKSPLVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAM 347

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP ++KSG+CGTVVRNAEMKIVDP+TG SLPR+Q GEICIRGDQIMKGYLNDPE
Sbjct: 348  CLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPE 407

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT  TI+K+GWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEA+L+ HPNI+D
Sbjct: 408  ATARTIEKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLVNHPNISD 467

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVV MKDE AGEVPVAFV+RSNGS ITE E+K +IS QV+FYKRI +VFF ET+PK+PS
Sbjct: 468  AAVVPMKDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPS 527

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 528  GKILRKDLRAR 538


>gb|AGE10594.1| 4-coumarate CoA ligase [Lonicera japonica]
          Length = 538

 Score =  432 bits (1110), Expect = e-118
 Identities = 209/251 (83%), Positives = 236/251 (94%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAKSP+VDNYD+SS+RT+MSGAAP+GKELED VRAK PNAKLGQGYGMTEAGPVL+M
Sbjct: 281  VLAIAKSPIVDNYDMSSMRTMMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAM 340

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSG+CGTVVRNA MKIVDPETG+SLPRHQSGEICIRGDQIMKGYLNDP+
Sbjct: 341  CLAFAKEPFEIKSGACGTVVRNAVMKIVDPETGASLPRHQSGEICIRGDQIMKGYLNDPK 400

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            +T++TIDK+GWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEAML+ HPNI+D
Sbjct: 401  STELTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLLNHPNISD 460

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVV MK+E+AGEVPVAFV+RS  S ITE EIK++IS QVVFYKRI +VFF + IPKAPS
Sbjct: 461  AAVVPMKEESAGEVPVAFVVRSGNSNITEDEIKQFISKQVVFYKRIKRVFFLDAIPKAPS 520

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 521  GKILRKDLRAR 531


>ref|XP_006381817.1| hypothetical protein POPTR_0006s18490g [Populus trichocarpa]
            gi|550336573|gb|ERP59614.1| hypothetical protein
            POPTR_0006s18490g [Populus trichocarpa]
          Length = 543

 Score =  431 bits (1109), Expect = e-118
 Identities = 214/251 (85%), Positives = 234/251 (93%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLA+AK PVVD YDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGP LSM
Sbjct: 286  VLAVAKCPVVDKYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGP-LSM 344

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSG+CGTVVRNAEMKIVDP+TG SLPR+Q+GEICIRG QIMKGYLNDPE
Sbjct: 345  CLAFAKEPFEIKSGACGTVVRNAEMKIVDPDTGRSLPRNQAGEICIRGSQIMKGYLNDPE 404

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT+ T+D DGWLHTGDIGYID ++E+FIVDRLKELIKYKGFQVAPAELEAMLIAHP+I+D
Sbjct: 405  ATERTVDNDGWLHTGDIGYIDGDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPDISD 464

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
             AVV MKDEAAGEVP+AFV+R+NGS+ITE EIK+YIS QVVFYKRI +VFF E IPKAPS
Sbjct: 465  CAVVPMKDEAAGEVPIAFVVRANGSKITEDEIKQYISKQVVFYKRISRVFFTEAIPKAPS 524

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 525  GKILRKDLRAR 535


>gb|AFG26323.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
          Length = 539

 Score =  431 bits (1109), Expect = e-118
 Identities = 210/251 (83%), Positives = 236/251 (94%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAKSP VD YDLSSIRTVMSGAAPMGKELED +RAK+PNAKLGQGYGMTEAGPVLSM
Sbjct: 282  VLAIAKSPEVDRYDLSSIRTVMSGAAPMGKELEDILRAKIPNAKLGQGYGMTEAGPVLSM 341

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSGSCGTVVRNAE+KIVDPETG+SLPR+Q+GEICIRG QIMKGYLNDPE
Sbjct: 342  CLAFAKEPFEIKSGSCGTVVRNAELKIVDPETGASLPRNQAGEICIRGSQIMKGYLNDPE 401

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            ATK+TIDK+GWLHTGDIG++DD++EIFIVDRLKELIKYKGFQVAPAELEAMLI HPN+ D
Sbjct: 402  ATKMTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLITHPNVAD 461

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVV MKD  AGEVPVAFV++SN S ITE+EIK++++ QVVFYKRI++VFF ++IPKAPS
Sbjct: 462  AAVVPMKDVLAGEVPVAFVVKSNSSNITEEEIKQFVAKQVVFYKRINRVFFVDSIPKAPS 521

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 522  GKILRKDLRAR 532


>ref|XP_007014191.1| 4-coumarate:CoA ligase 2 [Theobroma cacao]
            gi|508784554|gb|EOY31810.1| 4-coumarate:CoA ligase 2
            [Theobroma cacao]
          Length = 542

 Score =  430 bits (1106), Expect = e-118
 Identities = 215/251 (85%), Positives = 234/251 (93%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAK+P VD YDLSSIRTVMSGAAPMGKELED VRA+LPNAKLGQGYGMTEAGPVL+M
Sbjct: 285  VLAIAKTPDVDKYDLSSIRTVMSGAAPMGKELEDAVRARLPNAKLGQGYGMTEAGPVLAM 344

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
             LAFAKE  E KSGSCGTVVRNAEMKIV+P+TG+SLPR+QSGEICIRG QIMKGYLNDPE
Sbjct: 345  NLAFAKEACETKSGSCGTVVRNAEMKIVNPDTGASLPRNQSGEICIRGSQIMKGYLNDPE 404

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT+ TIDKDGWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEAMLI HPNI+D
Sbjct: 405  ATQRTIDKDGWLHTGDIGYIDDDDEVFIVDRLKELIKYKGFQVAPAELEAMLIGHPNISD 464

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVV MKDEAAGEVPVAFV+RSNGS+ITE EIK++IS QVVFYKR+ +VFF E IPKAPS
Sbjct: 465  AAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQFISKQVVFYKRLGQVFFTEAIPKAPS 524

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 525  GKILRKDLRAR 535


>sp|O24540.1|4CL_VANPL RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName:
            Full=4-coumaroyl-CoA synthase
          Length = 553

 Score =  430 bits (1106), Expect = e-118
 Identities = 211/251 (84%), Positives = 233/251 (92%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLAIAKS VVDNYDLSS+RTVMSGAAP+GKELED VRAK PNAKLGQGYGMTEAGPVL+M
Sbjct: 294  VLAIAKSTVVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAM 353

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP ++KSG+CGTVVRNAEMKIVDPETGSSLPR+  GEICIRGDQIMKGYLNDPE
Sbjct: 354  CLAFAKEPFDIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPE 413

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT  TIDK+GWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEA+L+ HP I+D
Sbjct: 414  ATARTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISD 473

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVV MKDEAAGEVPVAFV++SNG  ITE EIK++IS QV+FYKRI++VFF E IPKAPS
Sbjct: 474  AAVVPMKDEAAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPS 533

Query: 723  GKILRNDLRAK 755
            GKILR DLRA+
Sbjct: 534  GKILRKDLRAR 544


>gb|AEM63673.1| 4-hydroxycinnamoyl CoA ligase [Platycodon grandiflorus]
          Length = 556

 Score =  430 bits (1106), Expect = e-118
 Identities = 212/251 (84%), Positives = 237/251 (94%)
 Frame = +3

Query: 3    VLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSM 182
            VLA+AKS  VD YDL+SIRTVMSGAAP+GKELEDTVRAKLPNAKLGQGYGMTEAGPVL+M
Sbjct: 299  VLAMAKSSHVDKYDLTSIRTVMSGAAPLGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAM 358

Query: 183  CLAFAKEPSEVKSGSCGTVVRNAEMKIVDPETGSSLPRHQSGEICIRGDQIMKGYLNDPE 362
            CLAFAKEP E+KSG+CGTVVRNAEMKIVDP+TG SLPR+Q GEICIRGDQIMKGYLNDPE
Sbjct: 359  CLAFAKEPYEIKSGACGTVVRNAEMKIVDPDTGDSLPRNQRGEICIRGDQIMKGYLNDPE 418

Query: 363  ATKITIDKDGWLHTGDIGYIDDEEEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNIND 542
            AT+ TIDK+GWLHTGDIGYIDD++E+FIVDRLKELIKYKGFQVAPAELEA+L+ HPNI+D
Sbjct: 419  ATERTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISD 478

Query: 543  AAVVSMKDEAAGEVPVAFVIRSNGSQITEQEIKKYISDQVVFYKRIHKVFFPETIPKAPS 722
            AAVVSM DEAAGEVPVAFV+R+NGS ITE EIK++IS QVVFYKRI++VFF +TIPKAPS
Sbjct: 479  AAVVSMIDEAAGEVPVAFVVRANGSIITEDEIKQFISKQVVFYKRINRVFFTDTIPKAPS 538

Query: 723  GKILRNDLRAK 755
            GKILR +LRA+
Sbjct: 539  GKILRKELRAR 549