BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver27_contig00029218
         (826 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_007037234.1| Isochorismate synthase 2 isoform 5 [Theobrom...   344   3e-92
ref|XP_007037232.1| Isochorismate synthase 2 isoform 3 [Theobrom...   344   3e-92
ref|XP_007037230.1| Isochorismate synthase 2 isoform 1 [Theobrom...   344   3e-92
ref|XP_006476651.1| PREDICTED: isochorismate synthase, chloropla...   340   5e-91
ref|XP_006476649.1| PREDICTED: isochorismate synthase, chloropla...   340   5e-91
gb|EXB20726.1| Isochorismate synthase [Morus notabilis]               337   2e-90
ref|XP_004301164.1| PREDICTED: isochorismate synthase 2, chlorop...   335   9e-90
ref|XP_006439648.1| hypothetical protein CICLE_v10024236mg [Citr...   335   1e-89
ref|XP_007209084.1| hypothetical protein PRUPE_ppa003652mg [Prun...   333   6e-89
ref|XP_002267681.2| PREDICTED: isochorismate synthase 2, chlorop...   333   6e-89
emb|CBI15264.3| unnamed protein product [Vitis vinifera]              333   6e-89
gb|ACZ50508.1| isochorismate synthase [Populus fremontii x Popul...   332   1e-88
gb|ACX46384.1| isochorismate synthase [Populus tremuloides]           331   2e-88
gb|ACX46383.1| isochorismate synthase [Populus trichocarpa] gi|2...   329   7e-88
ref|XP_002511526.1| isochorismate synthase, putative [Ricinus co...   328   1e-87
ref|XP_002317989.2| hypothetical protein POPTR_0012s07180g [Popu...   327   3e-87
emb|CAN77956.1| hypothetical protein VITISV_021244 [Vitis vinifera]   327   3e-87
ref|XP_003607323.1| Isochorismate synthase [Medicago truncatula]...   322   1e-85
ref|XP_003516902.1| PREDICTED: isochorismate synthase, chloropla...   320   5e-85
gb|AHF20190.1| isochorismate synthase, partial [Musa acuminata A...   319   7e-85

>ref|XP_007037234.1| Isochorismate synthase 2 isoform 5 [Theobroma cacao]
            gi|590667485|ref|XP_007037235.1| Isochorismate synthase 2
            isoform 5 [Theobroma cacao] gi|508774479|gb|EOY21735.1|
            Isochorismate synthase 2 isoform 5 [Theobroma cacao]
            gi|508774480|gb|EOY21736.1| Isochorismate synthase 2
            isoform 5 [Theobroma cacao]
          Length = 532

 Score =  344 bits (882), Expect = 3e-92
 Identities = 171/269 (63%), Positives = 207/269 (76%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW +AI +L     Q++SVV  LRKE    +I+S+NHVP+K+ WD AV +AL+TIN S S
Sbjct: 250  TWDEAIDSLEATTHQVASVVVMLRKEASGELILSNNHVPNKTHWDLAVKRALQTINRSSS 309

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L+KV                         EG++AYQFC+QPPNAPAFIGNTPEQLFHRK
Sbjct: 310  PLIKVVLARSSRVLTATDIDPITWLACLQVEGENAYQFCLQPPNAPAFIGNTPEQLFHRK 369

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             L++SSEA+A TR RG +++LD Q+E+DLL SPKDHLEFTIVRE+I+ KLE +C+ V+VE
Sbjct: 370  WLSISSEAMAGTRARGGSRTLDLQIEHDLLSSPKDHLEFTIVRESIQNKLESVCDRVVVE 429

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P KTVRKL R+QHL+A+L+G L+ EDDEF +LS LHPTPAVCGFPTE AR FI+ETEMFD
Sbjct: 430  PKKTVRKLRRIQHLFAQLAGNLKREDDEFEILSYLHPTPAVCGFPTETARLFISETEMFD 489

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 490  RGMYAGPVGWFGGGESEFAVGIRSALVEK 518


>ref|XP_007037232.1| Isochorismate synthase 2 isoform 3 [Theobroma cacao]
            gi|590667479|ref|XP_007037233.1| Isochorismate synthase 2
            isoform 3 [Theobroma cacao] gi|508774477|gb|EOY21733.1|
            Isochorismate synthase 2 isoform 3 [Theobroma cacao]
            gi|508774478|gb|EOY21734.1| Isochorismate synthase 2
            isoform 3 [Theobroma cacao]
          Length = 523

 Score =  344 bits (882), Expect = 3e-92
 Identities = 171/269 (63%), Positives = 207/269 (76%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW +AI +L     Q++SVV  LRKE    +I+S+NHVP+K+ WD AV +AL+TIN S S
Sbjct: 250  TWDEAIDSLEATTHQVASVVVMLRKEASGELILSNNHVPNKTHWDLAVKRALQTINRSSS 309

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L+KV                         EG++AYQFC+QPPNAPAFIGNTPEQLFHRK
Sbjct: 310  PLIKVVLARSSRVLTATDIDPITWLACLQVEGENAYQFCLQPPNAPAFIGNTPEQLFHRK 369

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             L++SSEA+A TR RG +++LD Q+E+DLL SPKDHLEFTIVRE+I+ KLE +C+ V+VE
Sbjct: 370  WLSISSEAMAGTRARGGSRTLDLQIEHDLLSSPKDHLEFTIVRESIQNKLESVCDRVVVE 429

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P KTVRKL R+QHL+A+L+G L+ EDDEF +LS LHPTPAVCGFPTE AR FI+ETEMFD
Sbjct: 430  PKKTVRKLRRIQHLFAQLAGNLKREDDEFEILSYLHPTPAVCGFPTETARLFISETEMFD 489

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 490  RGMYAGPVGWFGGGESEFAVGIRSALVEK 518


>ref|XP_007037230.1| Isochorismate synthase 2 isoform 1 [Theobroma cacao]
            gi|590667451|ref|XP_007037231.1| Isochorismate synthase 2
            isoform 1 [Theobroma cacao] gi|508774475|gb|EOY21731.1|
            Isochorismate synthase 2 isoform 1 [Theobroma cacao]
            gi|508774476|gb|EOY21732.1| Isochorismate synthase 2
            isoform 1 [Theobroma cacao]
          Length = 561

 Score =  344 bits (882), Expect = 3e-92
 Identities = 171/269 (63%), Positives = 207/269 (76%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW +AI +L     Q++SVV  LRKE    +I+S+NHVP+K+ WD AV +AL+TIN S S
Sbjct: 250  TWDEAIDSLEATTHQVASVVVMLRKEASGELILSNNHVPNKTHWDLAVKRALQTINRSSS 309

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L+KV                         EG++AYQFC+QPPNAPAFIGNTPEQLFHRK
Sbjct: 310  PLIKVVLARSSRVLTATDIDPITWLACLQVEGENAYQFCLQPPNAPAFIGNTPEQLFHRK 369

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             L++SSEA+A TR RG +++LD Q+E+DLL SPKDHLEFTIVRE+I+ KLE +C+ V+VE
Sbjct: 370  WLSISSEAMAGTRARGGSRTLDLQIEHDLLSSPKDHLEFTIVRESIQNKLESVCDRVVVE 429

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P KTVRKL R+QHL+A+L+G L+ EDDEF +LS LHPTPAVCGFPTE AR FI+ETEMFD
Sbjct: 430  PKKTVRKLRRIQHLFAQLAGNLKREDDEFEILSYLHPTPAVCGFPTETARLFISETEMFD 489

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 490  RGMYAGPVGWFGGGESEFAVGIRSALVEK 518


>ref|XP_006476651.1| PREDICTED: isochorismate synthase, chloroplastic-like isoform X3
            [Citrus sinensis]
          Length = 572

 Score =  340 bits (871), Expect = 5e-91
 Identities = 170/270 (62%), Positives = 206/270 (76%), Gaps = 26/270 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW  AIS L   + ++SS + +L+KEV +T I+S++H PSK  WD AV KALE IN S +
Sbjct: 250  TWAKAISTLESTMDKVSSKIVRLQKEVSRTFILSNHHSPSKMHWDLAVKKALEIINRSST 309

Query: 645  ELVKV--------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHR 544
             L KV                          EG++AYQFC+QPP+APAFIGNTPEQLFHR
Sbjct: 310  PLTKVVLARSSRVITATDIDPIAWLACLKQVEGENAYQFCLQPPDAPAFIGNTPEQLFHR 369

Query: 543  KRLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLV 364
            K L ++SEALA TRGRG T +LD Q+E+DLL SPKDHLEFTIVRE+IR KLE +CN V++
Sbjct: 370  KWLKITSEALAGTRGRGGTTALDLQIEHDLLSSPKDHLEFTIVRESIRSKLEAVCNRVVI 429

Query: 363  EPLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMF 184
            EP K +RKLPRVQHLYA+L+GRLR+ED+E+ +++SLHP+PAVCGFPTE AR  IAETE+F
Sbjct: 430  EPKKAIRKLPRVQHLYARLAGRLRSEDEEYDIMASLHPSPAVCGFPTEEARLLIAETEVF 489

Query: 183  DRGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            DRGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 490  DRGMYAGPVGWFGGGESEFAVGIRSALVEK 519


>ref|XP_006476649.1| PREDICTED: isochorismate synthase, chloroplastic-like isoform X1
            [Citrus sinensis] gi|568845582|ref|XP_006476650.1|
            PREDICTED: isochorismate synthase, chloroplastic-like
            isoform X2 [Citrus sinensis]
          Length = 576

 Score =  340 bits (871), Expect = 5e-91
 Identities = 170/270 (62%), Positives = 206/270 (76%), Gaps = 26/270 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW  AIS L   + ++SS + +L+KEV +T I+S++H PSK  WD AV KALE IN S +
Sbjct: 254  TWAKAISTLESTMDKVSSKIVRLQKEVSRTFILSNHHSPSKMHWDLAVKKALEIINRSST 313

Query: 645  ELVKV--------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHR 544
             L KV                          EG++AYQFC+QPP+APAFIGNTPEQLFHR
Sbjct: 314  PLTKVVLARSSRVITATDIDPIAWLACLKQVEGENAYQFCLQPPDAPAFIGNTPEQLFHR 373

Query: 543  KRLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLV 364
            K L ++SEALA TRGRG T +LD Q+E+DLL SPKDHLEFTIVRE+IR KLE +CN V++
Sbjct: 374  KWLKITSEALAGTRGRGGTTALDLQIEHDLLSSPKDHLEFTIVRESIRSKLEAVCNRVVI 433

Query: 363  EPLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMF 184
            EP K +RKLPRVQHLYA+L+GRLR+ED+E+ +++SLHP+PAVCGFPTE AR  IAETE+F
Sbjct: 434  EPKKAIRKLPRVQHLYARLAGRLRSEDEEYDIMASLHPSPAVCGFPTEEARLLIAETEVF 493

Query: 183  DRGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            DRGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 494  DRGMYAGPVGWFGGGESEFAVGIRSALVEK 523


>gb|EXB20726.1| Isochorismate synthase [Morus notabilis]
          Length = 593

 Score =  337 bits (865), Expect = 2e-90
 Identities = 170/268 (63%), Positives = 205/268 (76%), Gaps = 25/268 (9%)
 Frame = -2

Query: 822  WQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKSE 643
            WQ AI+ L+  L Q+SS V KLRKE P+T I+S+N++PSK  WD AV +AL+ I+ S S 
Sbjct: 277  WQKAINELQATLCQVSSKVVKLRKEAPRTFILSNNNIPSKKYWDLAVNRALQKISRSDSA 336

Query: 642  LVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRKR 538
            L KV                         EG++AYQFC+QPP+APAFIGNTPEQLFHRK 
Sbjct: 337  LTKVVLARSSRVVTATDVDPITWLACLQVEGENAYQFCLQPPDAPAFIGNTPEQLFHRKW 396

Query: 537  LNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVEP 358
            L++ S+A+A T  RGE+ + D Q+E DLL SPK HLEF+IVRE+I+ KLED+CN V+V+P
Sbjct: 397  LDIESDAMAGTCARGESMTADLQIELDLLSSPKYHLEFSIVRESIKKKLEDVCNFVVVDP 456

Query: 357  LKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFDR 178
             K VRKLPR+QHLYAKL+GRLR+EDDEF +LSSLHPTPAVCGFPT+ AR  IAETE+FDR
Sbjct: 457  KKAVRKLPRIQHLYAKLAGRLRSEDDEFEILSSLHPTPAVCGFPTDEARLLIAETEIFDR 516

Query: 177  GMYAGPVGWFGGKETEFAVGIRSALVEK 94
            GMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 517  GMYAGPVGWFGGGESEFAVGIRSALVEK 544


>ref|XP_004301164.1| PREDICTED: isochorismate synthase 2, chloroplastic-like [Fragaria
            vesca subsp. vesca]
          Length = 514

 Score =  335 bits (860), Expect = 9e-90
 Identities = 174/269 (64%), Positives = 206/269 (76%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            T  +AI  L+  L QISS+V K +KEVP  +++S++H+PSK+ WD AV +AL+ I  S S
Sbjct: 204  TLGNAIGELKATLNQISSIVIKPQKEVPTLLMLSNSHIPSKTYWDLAVNRALQKIR-SDS 262

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L+KV                         EG++AYQFC+QPP APAFIGNTPEQLFHR 
Sbjct: 263  ALIKVVLARSSRVITATDIDPIAWLACLQVEGENAYQFCLQPPGAPAFIGNTPEQLFHRT 322

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             L+++SEALA TR RG++ S D Q+E DLL SPKDHLEFTIVRE+IR KLED+CN V+VE
Sbjct: 323  WLDINSEALAGTRARGKSLSEDLQIELDLLSSPKDHLEFTIVRESIRKKLEDVCNSVVVE 382

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P K +RKLPRVQHLYAKLSGRLR+EDDEF +LSSLHP+PAVCGFPTE AR  IAETE+FD
Sbjct: 383  PKKEIRKLPRVQHLYAKLSGRLRSEDDEFEILSSLHPSPAVCGFPTEEARVLIAETEVFD 442

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 443  RGMYAGPVGWFGGGESEFAVGIRSALVEK 471


>ref|XP_006439648.1| hypothetical protein CICLE_v10024236mg [Citrus clementina]
            gi|557541910|gb|ESR52888.1| hypothetical protein
            CICLE_v10024236mg [Citrus clementina]
          Length = 572

 Score =  335 bits (859), Expect = 1e-89
 Identities = 168/270 (62%), Positives = 205/270 (75%), Gaps = 26/270 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW  AIS L   + ++SS + +L+KEV +T I+S++H P K  WD AV KALE IN S +
Sbjct: 250  TWAKAISTLESTMDKVSSKIVRLQKEVSRTFILSNHHSPRKMHWDLAVKKALEIINRSST 309

Query: 645  ELVKV--------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHR 544
             L KV                          EG++AYQFC+QPP+APAFIGNTPEQLFHR
Sbjct: 310  PLTKVVLGRSSRVITATDIDPIAWLACLKQVEGENAYQFCLQPPDAPAFIGNTPEQLFHR 369

Query: 543  KRLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLV 364
            K L ++SEALA TRGRG T +LD Q+E+DL+ SPKDHLEFTIVRE+IR KLE IC+ V++
Sbjct: 370  KWLKITSEALAGTRGRGGTTALDLQIEHDLVSSPKDHLEFTIVRESIRSKLEAICDRVVI 429

Query: 363  EPLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMF 184
            EP K +RKLPRVQHLYA+L+GRLR+ED+E+ +++SLHP+PAVCGFPTE AR  IAETE+F
Sbjct: 430  EPKKAIRKLPRVQHLYARLAGRLRSEDEEYDIMASLHPSPAVCGFPTEEARLLIAETEVF 489

Query: 183  DRGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            DRGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 490  DRGMYAGPVGWFGGGESEFAVGIRSALVEK 519


>ref|XP_007209084.1| hypothetical protein PRUPE_ppa003652mg [Prunus persica]
            gi|462404819|gb|EMJ10283.1| hypothetical protein
            PRUPE_ppa003652mg [Prunus persica]
          Length = 559

 Score =  333 bits (853), Expect = 6e-89
 Identities = 172/265 (64%), Positives = 205/265 (77%), Gaps = 25/265 (9%)
 Frame = -2

Query: 813  AISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKSELVK 634
            AI AL+  L QISSVV K R++VP T ++S+NH+PSK+ W+ AV +AL+ I  S S L+K
Sbjct: 250  AIDALKATLSQISSVVLKQRRQVPWTPMLSNNHIPSKTYWNLAVNRALQKIR-SNSALIK 308

Query: 633  V-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRKRLNV 529
            V                         EG++AYQFC+QPPNAPAFIGNTPEQLFHRK L +
Sbjct: 309  VVLARRSTVITATDIDPIAWLACLQVEGENAYQFCLQPPNAPAFIGNTPEQLFHRKWLGI 368

Query: 528  SSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVEPLKT 349
            +SEALA TR RG++   D Q+E +LL SPKDHLEFTIV+E+IR KLE++CN VLVEP K 
Sbjct: 369  TSEALAGTRARGKSMPEDLQIELELLSSPKDHLEFTIVQESIRRKLENVCNSVLVEPKKA 428

Query: 348  VRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFDRGMY 169
            +RKLPRVQHLYAKL+GRLR+E+DEF +LSSLHP+PAVCGFPTE AR  IAETE+FDRGMY
Sbjct: 429  IRKLPRVQHLYAKLAGRLRSEEDEFDILSSLHPSPAVCGFPTEEARVLIAETEVFDRGMY 488

Query: 168  AGPVGWFGGKETEFAVGIRSALVEK 94
            AGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 489  AGPVGWFGGGESEFAVGIRSALVEK 513


>ref|XP_002267681.2| PREDICTED: isochorismate synthase 2, chloroplastic [Vitis vinifera]
          Length = 560

 Score =  333 bits (853), Expect = 6e-89
 Identities = 172/269 (63%), Positives = 205/269 (76%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW+ AI AL+  + Q+SS+V KLRKEV KT ++S++HVPSK+SW+ AV +AL+ I  + S
Sbjct: 249  TWEKAIGALQATMCQVSSIVGKLRKEVCKTFVLSNSHVPSKASWNLAVNRALQIIGRADS 308

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L+KV                         EGQ+AYQF +QPP+APAFIGNTPEQLFHRK
Sbjct: 309  PLIKVVLARSSRVVTTSHIDPIAWLACLQVEGQNAYQFFLQPPDAPAFIGNTPEQLFHRK 368

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
            RL + SEALA TR RG +K+ D Q+E DLL SPK+  EF+IVRE+I+ KLE IC+ V VE
Sbjct: 369  RLCIHSEALAGTRARGGSKAQDLQIELDLLSSPKEDFEFSIVRESIQRKLEAICSTVWVE 428

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P K +RKLPR+QHLYA+LSGRLR+EDDEF +LSSLHPTPAVCGFP E AR  IAETEMFD
Sbjct: 429  PKKAIRKLPRIQHLYAQLSGRLRSEDDEFDILSSLHPTPAVCGFPREEARLLIAETEMFD 488

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGM+AGPVGWFGG ETEFAVGIRSALVEK
Sbjct: 489  RGMFAGPVGWFGGGETEFAVGIRSALVEK 517


>emb|CBI15264.3| unnamed protein product [Vitis vinifera]
          Length = 511

 Score =  333 bits (853), Expect = 6e-89
 Identities = 172/269 (63%), Positives = 205/269 (76%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW+ AI AL+  + Q+SS+V KLRKEV KT ++S++HVPSK+SW+ AV +AL+ I  + S
Sbjct: 200  TWEKAIGALQATMCQVSSIVGKLRKEVCKTFVLSNSHVPSKASWNLAVNRALQIIGRADS 259

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L+KV                         EGQ+AYQF +QPP+APAFIGNTPEQLFHRK
Sbjct: 260  PLIKVVLARSSRVVTTSHIDPIAWLACLQVEGQNAYQFFLQPPDAPAFIGNTPEQLFHRK 319

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
            RL + SEALA TR RG +K+ D Q+E DLL SPK+  EF+IVRE+I+ KLE IC+ V VE
Sbjct: 320  RLCIHSEALAGTRARGGSKAQDLQIELDLLSSPKEDFEFSIVRESIQRKLEAICSTVWVE 379

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P K +RKLPR+QHLYA+LSGRLR+EDDEF +LSSLHPTPAVCGFP E AR  IAETEMFD
Sbjct: 380  PKKAIRKLPRIQHLYAQLSGRLRSEDDEFDILSSLHPTPAVCGFPREEARLLIAETEMFD 439

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGM+AGPVGWFGG ETEFAVGIRSALVEK
Sbjct: 440  RGMFAGPVGWFGGGETEFAVGIRSALVEK 468


>gb|ACZ50508.1| isochorismate synthase [Populus fremontii x Populus angustifolia]
          Length = 572

 Score =  332 bits (850), Expect = 1e-88
 Identities = 167/269 (62%), Positives = 201/269 (74%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW+ A+ A+   + QISS V KL KEV ++ I+S++HVP K  WD AV +AL+ IN S S
Sbjct: 251  TWEQAVDAVEATMTQISSNVVKLSKEVTRSFILSNSHVPCKMYWDLAVERALQIINRSSS 310

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L KV                         EG++AYQFC+QPPNAPAFIGNTPEQLFHR 
Sbjct: 311  PLTKVVLARSSKFVIANDIDPIAWLACLQVEGENAYQFCLQPPNAPAFIGNTPEQLFHRN 370

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             L +SS+A+A TR RG + +LD Q++ DLL SPKDHLEFT VR++IR KLE +C+ ++VE
Sbjct: 371  CLGISSDAMAGTRARGGSMALDLQIQLDLLSSPKDHLEFTTVRDDIRKKLEAVCDRIVVE 430

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P K +RKL RVQHLYA+L+G LRNEDDEF++LSSLHPTPAVCGFPTE AR  IAETE+FD
Sbjct: 431  PTKAIRKLHRVQHLYARLAGELRNEDDEFKILSSLHPTPAVCGFPTEEARLLIAETEVFD 490

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 491  RGMYAGPVGWFGGGESEFAVGIRSALVEK 519


>gb|ACX46384.1| isochorismate synthase [Populus tremuloides]
          Length = 572

 Score =  331 bits (849), Expect = 2e-88
 Identities = 168/269 (62%), Positives = 200/269 (74%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW+ A+ A+   + QISS V KL KEV ++ IISS+H+P K  WD AV +AL+ IN S S
Sbjct: 251  TWEQAVDAVEATMTQISSNVVKLSKEVTRSFIISSSHIPCKMYWDLAVERALQIINRSSS 310

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L KV                         EG++AYQFC+QPPNAPAFIGNTPEQLFHR 
Sbjct: 311  PLTKVVLARSSKIVIANDIDPIAWLACLQVEGENAYQFCLQPPNAPAFIGNTPEQLFHRN 370

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             L +SSEA+A TR RG + +LD Q++ DLL SPKDHLEFTIVR+NIR KLE +C+ ++VE
Sbjct: 371  CLGISSEAMAGTRARGGSMALDLQIQLDLLSSPKDHLEFTIVRDNIRKKLEAVCDRIVVE 430

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P K +RK  RVQHLYA+L+G+LR+EDDEF +LSSLHPTPAVCGFPTE AR  IAETE+FD
Sbjct: 431  PNKAIRKFHRVQHLYARLAGQLRSEDDEFNILSSLHPTPAVCGFPTEEARLLIAETEVFD 490

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RG YAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 491  RGKYAGPVGWFGGGESEFAVGIRSALVEK 519


>gb|ACX46383.1| isochorismate synthase [Populus trichocarpa]
            gi|269851435|gb|ACZ50509.1| isochorismate synthase
            [Populus fremontii x Populus angustifolia]
            gi|269851437|gb|ACZ50510.1| isochorismate synthase
            [Populus fremontii x Populus angustifolia]
          Length = 572

 Score =  329 bits (844), Expect = 7e-88
 Identities = 167/269 (62%), Positives = 200/269 (74%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW+ A+ A+   + QISS V KL KEV ++ I+S++HVP K  WD AV +AL+ IN S S
Sbjct: 251  TWEQAVDAVEATMTQISSNVLKLSKEVTRSFILSNSHVPCKMYWDLAVERALQIINRSSS 310

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L KV                         EG++AYQFC+QPPNAPAFIGNTPEQLFHR 
Sbjct: 311  PLTKVVLARSSKFVIGNDIDPIAWLACLQVEGENAYQFCLQPPNAPAFIGNTPEQLFHRN 370

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             L +SSEA+A TR RG + +LD Q++ DLL SPKDHLEFTIVR+NIR KLE +C+ ++VE
Sbjct: 371  CLGISSEAMAGTRVRGGSMALDLQIQLDLLSSPKDHLEFTIVRDNIRKKLEAVCDRIVVE 430

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P K +RK  RVQHLYA+L+G LR+EDDEF++LSSLHPTPAVCGFPTE AR  IAETE+FD
Sbjct: 431  PNKAIRKFHRVQHLYARLAGELRSEDDEFKILSSLHPTPAVCGFPTEEARLLIAETEVFD 490

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGMYAGPVGWFGG E+EFAVGIRSAL EK
Sbjct: 491  RGMYAGPVGWFGGGESEFAVGIRSALAEK 519


>ref|XP_002511526.1| isochorismate synthase, putative [Ricinus communis]
            gi|223550641|gb|EEF52128.1| isochorismate synthase,
            putative [Ricinus communis]
          Length = 556

 Score =  328 bits (841), Expect = 1e-87
 Identities = 170/269 (63%), Positives = 198/269 (73%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW+ AI AL+  + QISS   KL KE  +T I++ NH PSK+ WD AV +AL+ IN   S
Sbjct: 246  TWEQAIDALQLTMSQISSTAVKLCKEFSRTFILNINHFPSKTYWDLAVKRALQIINRRSS 305

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L KV                         EG++AYQFC+QPPN+PAFIGNTPEQL HRK
Sbjct: 306  PLTKVVLARSSRVVTANDIDPITWLACLQVEGENAYQFCLQPPNSPAFIGNTPEQLLHRK 365

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             L VSSEALA TR RG +K+LD Q+E DLL SPKDHLEF IVR++IR KLE +C+ V++E
Sbjct: 366  WLGVSSEALAGTRARGGSKALDLQIELDLLSSPKDHLEFAIVRDSIRRKLEAMCSRVVIE 425

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P K +RK  RVQHLYA+LSG LR+EDDEF +LSSLHPTPAVCGFPTE AR  IAETE+FD
Sbjct: 426  PNKAIRKFSRVQHLYAQLSGTLRSEDDEFDILSSLHPTPAVCGFPTEEARLLIAETEVFD 485

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 486  RGMYAGPVGWFGGGESEFAVGIRSALVEK 514


>ref|XP_002317989.2| hypothetical protein POPTR_0012s07180g [Populus trichocarpa]
            gi|550326564|gb|EEE96209.2| hypothetical protein
            POPTR_0012s07180g [Populus trichocarpa]
          Length = 572

 Score =  327 bits (839), Expect = 3e-87
 Identities = 166/269 (61%), Positives = 200/269 (74%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW+ A+ A+   + QISS V KL KEV ++ I+S++HVP K  WD AV +AL+ IN S S
Sbjct: 251  TWEQAVDAVEATMTQISSNVLKLSKEVTRSFILSNSHVPCKMYWDLAVERALQIINRSSS 310

Query: 645  ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L KV                         EG++AYQFC+QPPNAPAFIGNT +QLFHR 
Sbjct: 311  PLTKVVLARSSKFVIGNDIDPIAWLACLQVEGENAYQFCLQPPNAPAFIGNTAKQLFHRN 370

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             L +SSEA+A TR RG + +LD Q++ DLL SPKDHLEFTIVR+NIR KLE +C+ ++VE
Sbjct: 371  CLGISSEAMAGTRARGGSMALDLQIQLDLLSSPKDHLEFTIVRDNIRKKLEAVCDRIVVE 430

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P K +RK  RVQHLYA+L+G LR+EDDEF++LSSLHPTPAVCGFPTE AR  IAETE+FD
Sbjct: 431  PNKAIRKFHRVQHLYARLAGELRSEDDEFKILSSLHPTPAVCGFPTEEARLLIAETEVFD 490

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 491  RGMYAGPVGWFGGGESEFAVGIRSALVEK 519


>emb|CAN77956.1| hypothetical protein VITISV_021244 [Vitis vinifera]
          Length = 574

 Score =  327 bits (838), Expect = 3e-87
 Identities = 172/284 (60%), Positives = 205/284 (72%), Gaps = 40/284 (14%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            TW+ AI AL+  + Q+SS+V KLRKEV KT ++S++HVPSK+SW+ AV +AL+ I  + S
Sbjct: 249  TWEKAIGALQATMCQVSSIVGKLRKEVCKTFVLSNSHVPSKASWNLAVNRALQIIGRADS 308

Query: 645  ELVK----------------------------------------VEGQDAYQFCIQPPNA 586
             L+K                                        VEGQ+AYQF +QPP+A
Sbjct: 309  PLIKGQPVLVAARKMLQPLVVLARSSRVVTTSHIDPIAWLACLQVEGQNAYQFFLQPPDA 368

Query: 585  PAFIGNTPEQLFHRKRLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVREN 406
            PAFIGNTPEQLFHRKRL + SEALA TR RG +K+ D Q+E DLL SPK+  EF+IVRE+
Sbjct: 369  PAFIGNTPEQLFHRKRLCIHSEALAGTRARGGSKAQDLQIELDLLSSPKEDFEFSIVRES 428

Query: 405  IRGKLEDICNMVLVEPLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFP 226
            I+ KLE IC+ V VEP K +RKLPR+QHLYA+LSGRLR+EDDEF +LSSLHPTPAVCGFP
Sbjct: 429  IQRKLEAICSTVWVEPKKAIRKLPRIQHLYAQLSGRLRSEDDEFDILSSLHPTPAVCGFP 488

Query: 225  TEVARQFIAETEMFDRGMYAGPVGWFGGKETEFAVGIRSALVEK 94
             E AR  IAETEMFDRGM+AGPVGWFGG ETEFAVGIRSALVEK
Sbjct: 489  REEARLLIAETEMFDRGMFAGPVGWFGGGETEFAVGIRSALVEK 532


>ref|XP_003607323.1| Isochorismate synthase [Medicago truncatula]
            gi|355508378|gb|AES89520.1| Isochorismate synthase
            [Medicago truncatula]
          Length = 570

 Score =  322 bits (825), Expect = 1e-85
 Identities = 162/269 (60%), Positives = 203/269 (75%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            +W+ AI AL+  L ++S+   K  K+ P T+I+SS+++PSK  W+ AV +AL+ I    S
Sbjct: 253  SWEKAIVALQETLCKVSTSNVKFPKQAPPTLILSSHNIPSKEDWNLAVNRALQMIEQHDS 312

Query: 645  EL-------------------------VKVEGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L                         +KVEG +AYQF +QPPNAPAFIGNTPEQLFHRK
Sbjct: 313  SLTKVVLARSTRVVPTVNIDPLTWLACLKVEGGNAYQFFLQPPNAPAFIGNTPEQLFHRK 372

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
            RL+++SEALA TR RG + +LDRQ+E DLL SPKD +EFTIVRE+IR KLE +C  V++E
Sbjct: 373  RLHITSEALAGTRARGPSLALDRQIELDLLTSPKDDIEFTIVRESIRRKLEAVCEKVIIE 432

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P K +RKLPR+QHL+A+L+GRLRNE+DEF +LSSLHP+PAVCGFPTEVA+  IAETE+FD
Sbjct: 433  PKKMIRKLPRIQHLFAQLTGRLRNEEDEFEILSSLHPSPAVCGFPTEVAQVLIAETEVFD 492

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGMYAGPVGWFGG E+EFAVGIRSALVE+
Sbjct: 493  RGMYAGPVGWFGGGESEFAVGIRSALVEQ 521


>ref|XP_003516902.1| PREDICTED: isochorismate synthase, chloroplastic-like isoform X1
            [Glycine max] gi|571434867|ref|XP_006573318.1| PREDICTED:
            isochorismate synthase, chloroplastic-like isoform X2
            [Glycine max]
          Length = 563

 Score =  320 bits (819), Expect = 5e-85
 Identities = 161/269 (59%), Positives = 203/269 (75%), Gaps = 25/269 (9%)
 Frame = -2

Query: 825  TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
            +W++AI+AL+  LR +SS + K  K+ P T+I SS+++PSK  WD AV  AL+ I  + S
Sbjct: 246  SWENAINALQETLRNVSSSIVKFPKQTPPTLISSSHNIPSKVDWDLAVNGALQMIKRNDS 305

Query: 645  EL-------------------------VKVEGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
             L                         +KVEG++AYQF +QPPNAPAFIGNTPEQLFHRK
Sbjct: 306  LLTKVVLARSTRVVPTADIDPLAWLSCLKVEGENAYQFLLQPPNAPAFIGNTPEQLFHRK 365

Query: 540  RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             L+++SEALA TR RG +++LD Q+E DLL SPKD +EFTIVR+ IR KLE +C  V+++
Sbjct: 366  CLHITSEALAGTRARGASQALDCQIELDLLTSPKDDIEFTIVRDTIRRKLEAVCEKVVIK 425

Query: 360  PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
            P K +RKLPR+QHLY++LSGRLR+E+DEF +LSSLHP+PAVCGFPTE A+  IAETE+FD
Sbjct: 426  PEKMIRKLPRIQHLYSQLSGRLRSEEDEFEILSSLHPSPAVCGFPTEEAQLLIAETEVFD 485

Query: 180  RGMYAGPVGWFGGKETEFAVGIRSALVEK 94
            RGMYAGPVGWFGG E+EFAVGIRSALVEK
Sbjct: 486  RGMYAGPVGWFGGGESEFAVGIRSALVEK 514


>gb|AHF20190.1| isochorismate synthase, partial [Musa acuminata AAA Group]
          Length = 438

 Score =  319 bits (818), Expect = 7e-85
 Identities = 167/268 (62%), Positives = 197/268 (73%), Gaps = 25/268 (9%)
 Frame = -2

Query: 825 TWQDAISALRPELRQISSVVTKLRKEVPKTVIISSNHVPSKSSWDDAVGKALETINGSKS 646
           TWQ A+  L+  L+QISS   +LRK  PKT IIS  H PSK SWD +V +ALE +NG +S
Sbjct: 114 TWQKAMDELKATLQQISSCFGRLRKLDPKTAIISFQHFPSKGSWDISVKRALEIVNGRES 173

Query: 645 ELVKV-------------------------EGQDAYQFCIQPPNAPAFIGNTPEQLFHRK 541
           ELVKV                         E Q+AYQF IQPP APAFIGNTPEQLFHRK
Sbjct: 174 ELVKVVLARCSKYIIDTTMDPLVLLASLKAESQNAYQFYIQPPGAPAFIGNTPEQLFHRK 233

Query: 540 RLNVSSEALAATRGRGETKSLDRQMENDLLRSPKDHLEFTIVRENIRGKLEDICNMVLVE 361
             +V+SEALA TR RG TK+ D Q+  DLL SPKD++EF IVRE+IR KL+ IC+ V +E
Sbjct: 234 CFHVTSEALAGTRARGVTKAEDLQIGQDLLFSPKDNIEFVIVRESIRRKLQIICDEVHIE 293

Query: 360 PLKTVRKLPRVQHLYAKLSGRLRNEDDEFRVLSSLHPTPAVCGFPTEVARQFIAETEMFD 181
           P K +RKLPR+QHL A+LSGRLR+ED EF +L+SLHP+PAVCG PTE AR+FI E EMFD
Sbjct: 294 PSKALRKLPRIQHLSAQLSGRLRDEDSEFDILTSLHPSPAVCGLPTEEARRFIKENEMFD 353

Query: 180 RGMYAGPVGWFGGKETEFAVGIRSALVE 97
           RGMYAGPVGWFGG+E+EFAVGIRSALV+
Sbjct: 354 RGMYAGPVGWFGGRESEFAVGIRSALVK 381