BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver23_contig00023578
         (983 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin...   365   9e-99
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin...   344   2e-92
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi...   343   4e-92
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro...   339   5e-91
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      304   2e-80

>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
           Short=EcTNMT gi|226897702|gb|ACO90222.1|
           tetrahydroprotoberberine N-methyltransferase
           [Eschscholzia californica]
          Length = 350

 Score =  365 bits (937), Expect = 9e-99
 Identities = 181/272 (66%), Positives = 213/272 (78%)
 Frame = -3

Query: 981 ATLGSTLKQSACYFKEESMTIDEAEIAAYELDCERAQIKDGQTILDIGCGFGGLVLHIAQ 802
           A  G T+KQS CYFK+E+ TIDEAE AA+EL CERAQIKDGQT+LDIGCG GGLVL+IA+
Sbjct: 82  AVFGKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGGLVLYIAE 141

Query: 801 KYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVTKYEFENEKKFDRILVVEAIE 622
           KYKNCHVTGLTNS  Q NYIE Q EKL L+NVDVI ADVTK  F+ +K +DRILVVE IE
Sbjct: 142 KYKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVTK--FDTDKTYDRILVVETIE 199

Query: 621 HMKNIQQFLKKISKWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLS 442
           HMKNIQ F+KK+S WM  EDS LFV H+ HK F+ +FEALDEDDWY+ ++ P+G VT LS
Sbjct: 200 HMKNIQLFMKKLSTWMT-EDSLLFVDHISHKTFNHNFEALDEDDWYSGFIFPKGCVTILS 258

Query: 441 ASALLYFQDDVSVVDQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVN 262
           +S LLYFQDDVS +D W+++G HMARS E W K+    +EAA + L  GLGS+EA NQV 
Sbjct: 259 SSTLLYFQDDVSALDHWVVNGMHMARSVEAWRKKLDETIEAAREILEPGLGSKEAVNQVI 318

Query: 261 IQYKTFFMGYVVQFSYNNGEEWMISHYLFKKK 166
              +TF +G   QFSYNNGEEWMI+  LFKKK
Sbjct: 319 THIRTFCIGGYEQFSYNNGEEWMITQILFKKK 350


>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
           Short=PsTNMT gi|67764091|gb|AAY79177.1|
           S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
           cis-N-methyltransferase [Papaver somniferum]
          Length = 358

 Score =  344 bits (882), Expect = 2e-92
 Identities = 172/273 (63%), Positives = 207/273 (75%), Gaps = 1/273 (0%)
 Frame = -3

Query: 981 ATLGSTLKQSACYFKEESMTIDEAEIAAYELDCERAQIKDGQTILDIGCGFGGLVLHIAQ 802
           A  G T+KQS CYF  ES TIDEAE AA+EL CERAQIKDGQT+LDIGCG GGLVL+IAQ
Sbjct: 89  AVFGKTVKQSMCYFTHESATIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGGLVLYIAQ 148

Query: 801 KYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVTKYEFENEKKFDRILVVEAIE 622
           KYKNCHVTGLTNS  Q NY+  Q EKL L+NVD ILADVT+Y  E++K +DR+L++EAIE
Sbjct: 149 KYKNCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQY--ESDKTYDRLLMIEAIE 206

Query: 621 HMKNIQQFLKKISKWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLS 442
           HMKN+Q F+KK+S WM  E S LFV H+CHK F+  FEA+DEDDWY+ ++ P G  T L+
Sbjct: 207 HMKNLQLFMKKLSTWMTKE-SLLFVDHVCHKTFAHFFEAVDEDDWYSGFIFPPGCATILA 265

Query: 441 ASALLYFQDDVSVVDQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQV 265
           A++LLYFQDDVSVVD W+++G HMARS + W K    N+EAA + L  GL GS E  N V
Sbjct: 266 ANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHETVNGV 325

Query: 264 NIQYKTFFMGYVVQFSYNNGEEWMISHYLFKKK 166
               +TF MG   QFS NNG+EWM++  LFKKK
Sbjct: 326 VTHIRTFCMGGYEQFSMNNGDEWMVAQLLFKKK 358


>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
           Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
           tetrahydroprotoberberine N-methyltransferase [Papaver
           bracteatum]
          Length = 358

 Score =  343 bits (880), Expect = 4e-92
 Identities = 171/273 (62%), Positives = 209/273 (76%), Gaps = 1/273 (0%)
 Frame = -3

Query: 981 ATLGSTLKQSACYFKEESMTIDEAEIAAYELDCERAQIKDGQTILDIGCGFGGLVLHIAQ 802
           A  G T+KQS CYFK ES TIDEAE AA+EL CERAQIKDGQT+LDIGCG GGLVL+IA+
Sbjct: 89  AVFGKTVKQSMCYFKHESATIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGGLVLYIAR 148

Query: 801 KYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVTKYEFENEKKFDRILVVEAIE 622
           KYK CHVTGLTNS  Q NY+  Q EKL L+NVD ILADVT+Y  E++K +DR+L++EAIE
Sbjct: 149 KYKKCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQY--ESDKTYDRLLMIEAIE 206

Query: 621 HMKNIQQFLKKISKWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLS 442
           HMKN+Q F+KK+S WM  E+S LFV H+CHK F+  FEA+DEDDWY+ ++ P G  T L+
Sbjct: 207 HMKNLQLFMKKLSTWM-TEESLLFVDHVCHKTFAHFFEAVDEDDWYSGFIFPPGCATILA 265

Query: 441 ASALLYFQDDVSVVDQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQV 265
           A++LLYFQDDVSVVD W+++G HMARS + W K    N+EAA + L  GL GS EA N V
Sbjct: 266 ANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHEAVNGV 325

Query: 264 NIQYKTFFMGYVVQFSYNNGEEWMISHYLFKKK 166
               +TF MG   QFS N+G+EWM++  LFKKK
Sbjct: 326 VTHIRTFCMGGYEQFSMNDGDEWMVAQLLFKKK 358


>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
           N-methyltransferase 2; Short=PbTNMT2
           gi|226897730|gb|ACO90236.1| putative N-methyltransferase
           [Papaver bracteatum]
          Length = 358

 Score =  339 bits (870), Expect = 5e-91
 Identities = 170/273 (62%), Positives = 205/273 (75%), Gaps = 1/273 (0%)
 Frame = -3

Query: 981 ATLGSTLKQSACYFKEESMTIDEAEIAAYELDCERAQIKDGQTILDIGCGFGGLVLHIAQ 802
           A  G ++K S CYFK ES TIDEAE A++EL CERAQIKDGQT+LDIGCG GGLVL++AQ
Sbjct: 89  AAYGKSMKLSGCYFKHESSTIDEAEEASHELYCERAQIKDGQTVLDIGCGQGGLVLYVAQ 148

Query: 801 KYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVTKYEFENEKKFDRILVVEAIE 622
           KYKNCHVTGLTNS EQ NYI  Q EKL L NVDVILADVT+Y  E++K +DRILV+  +E
Sbjct: 149 KYKNCHVTGLTNSKEQVNYILKQAEKLGLRNVDVILADVTQY--ESDKTYDRILVIGVVE 206

Query: 621 HMKNIQQFLKKISKWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLS 442
           HMKN+Q F+KK+S WM  EDS LFV H CHK F+  FEALDEDDWY+ Y+ P G  T+LS
Sbjct: 207 HMKNMQLFIKKLSTWMA-EDSLLFVDHSCHKTFNHFFEALDEDDWYSGYIFPPGCATFLS 265

Query: 441 ASALLYFQDDVSVVDQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQV 265
           A +LLYFQDDVSVVD W+++G H AR+ + W K+   N+EA  + L  GL G+ EA N V
Sbjct: 266 ADSLLYFQDDVSVVDHWVVNGMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGV 325

Query: 264 NIQYKTFFMGYVVQFSYNNGEEWMISHYLFKKK 166
               +T  +G  VQFS N+G+EWM +  LFKKK
Sbjct: 326 ITHIRTCCVGGYVQFSLNDGDEWMNAQLLFKKK 358


>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  304 bits (778), Expect = 2e-80
 Identities = 146/269 (54%), Positives = 189/269 (70%)
 Frame = -3

Query: 972 GSTLKQSACYFKEESMTIDEAEIAAYELDCERAQIKDGQTILDIGCGFGGLVLHIAQKYK 793
           GS LK S CYFKE+S T+DEAEIA  +L CERAQI+DGQ++LD+GCG G L LH+AQKYK
Sbjct: 93  GSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVLDLGCGQGALTLHVAQKYK 152

Query: 792 NCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVTKYEFENEKKFDRILVVEAIEHMK 613
           NC VT +TNS  Q+ YIE +  + NL NV+V LAD+T +E    + +DRILV+E  EHMK
Sbjct: 153 NCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMA--ETYDRILVIELFEHMK 210

Query: 612 NIQQFLKKISKWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASA 433
           N +  L+KIS+W+  +D  LF+ H+CHK F+ H+E LD+DDW+T YV P G++   SAS 
Sbjct: 211 NYELLLRKISEWIS-KDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVFPAGTMIIPSASF 269

Query: 432 LLYFQDDVSVVDQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQY 253
            LYFQDDVSVV+ W LSG H +R+ EEW KR   NL+         +G+EE A ++   +
Sbjct: 270 FLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVKLINYW 329

Query: 252 KTFFMGYVVQFSYNNGEEWMISHYLFKKK 166
           + F +  +  F YNNGEEWM SH LFKKK
Sbjct: 330 RGFCLSGMEMFGYNNGEEWMASHVLFKKK 358