BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver23_contig00022013
         (716 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName...   355   5e-96
gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]          290   3e-76
gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]          279   4e-73
pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a ...   271   7e-71
pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Comp...   271   7e-71

>sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName: Full=Berberine
           bridge-forming enzyme; Short=BBE; AltName:
           Full=Tetrahydroprotoberberine synthase; Flags: Precursor
           gi|3282517|gb|AAC61839.1| berberine bridge enzyme
           [Papaver somniferum]
          Length = 535

 Score =  355 bits (911), Expect = 5e-96
 Identities = 169/181 (93%), Positives = 176/181 (97%)
 Frame = -1

Query: 716 FDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRK 537
           FD+RAFKTKVDFTK +VPL+VFR ALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRK
Sbjct: 355 FDERAFKTKVDFTKVSVPLNVFRHALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRK 414

Query: 536 GTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWK 357
           GTKLMFEYIIAWNQDEE+KIGEFSEWL KFYDYLEPFVSKEPRVGYVNHIDLDIGGIDW+
Sbjct: 415 GTKLMFEYIIAWNQDEESKIGEFSEWLAKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWR 474

Query: 356 NKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEENYILNE 177
           NKSST+NAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEE Y+L E
Sbjct: 475 NKSSTTNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEEIYMLKE 534

Query: 176 L 174
           L
Sbjct: 535 L 535


>gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]
          Length = 554

 Score =  290 bits (741), Expect = 3e-76
 Identities = 131/172 (76%), Positives = 153/172 (88%)
 Frame = -1

Query: 716 FDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRK 537
           FDDRAFKTKVDFTKE +PL+     LE+LS++P GFIALNGFGGKMS+IS DFTPFPHRK
Sbjct: 363 FDDRAFKTKVDFTKETLPLEAIDGLLEILSKEPRGFIALNGFGGKMSKISNDFTPFPHRK 422

Query: 536 GTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWK 357
           GTKLM EYI+AW++DEE+K  EF +WL   YDY+E FVSK PRVGYVNHIDLD+GGIDW 
Sbjct: 423 GTKLMVEYIVAWSKDEESKSDEFFDWLRNIYDYMEMFVSKNPRVGYVNHIDLDLGGIDWS 482

Query: 356 NKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKF 201
           +K+S++NA+EIARNWGE+YF SNYERL++AKTLIDPNNVFNHPQSIPPMMK+
Sbjct: 483 DKNSSNNAIEIARNWGEKYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMMKY 534


>gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]
          Length = 536

 Score =  279 bits (714), Expect = 4e-73
 Identities = 125/172 (72%), Positives = 149/172 (86%)
 Frame = -1

Query: 713 DDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKG 534
           DD+AFKTKVDFTKE +PL V    LE+LS++P GFI LN  GGKMS+IS DFTPFPHR G
Sbjct: 359 DDKAFKTKVDFTKETLPLKVINGVLEILSKEPRGFILLNSLGGKMSKISNDFTPFPHRNG 418

Query: 533 TKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKN 354
           TKLM EYI++W++DEE+K  EF +WL   YDY+E FVSK PRVGYVN+IDLD+GGIDW N
Sbjct: 419 TKLMVEYIVSWSKDEESKSDEFFDWLRNIYDYMEEFVSKNPRVGYVNNIDLDLGGIDWSN 478

Query: 353 KSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 198
           K+S++NA+EIARNWGE+YF SNYERL++AKTLIDPNN+FNHPQSIPPMMKF+
Sbjct: 479 KNSSNNAIEIARNWGEKYFLSNYERLIRAKTLIDPNNIFNHPQSIPPMMKFD 530


>pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In
           Complex With (S)-Reticuline
          Length = 519

 Score =  271 bits (694), Expect = 7e-71
 Identities = 122/173 (70%), Positives = 147/173 (84%)
 Frame = -1

Query: 716 FDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRK 537
           FD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR 
Sbjct: 332 FDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRS 391

Query: 536 GTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWK 357
           GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW 
Sbjct: 392 GTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWG 451

Query: 356 NKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 198
           NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM  F+
Sbjct: 452 NKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANFD 504


>pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
           Dehydroscoulerine
          Length = 519

 Score =  271 bits (694), Expect = 7e-71
 Identities = 122/173 (70%), Positives = 147/173 (84%)
 Frame = -1

Query: 716 FDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRK 537
           FD+RAFKTKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR 
Sbjct: 332 FDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRS 391

Query: 536 GTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWK 357
           GT+LM EYI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW 
Sbjct: 392 GTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWG 451

Query: 356 NKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 198
           NK+  +NA+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM  F+
Sbjct: 452 NKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANFD 504