BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver23_contig00006925
         (1630 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   688   0.0  
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      469   e-130
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   464   e-128
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       442   e-122
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   428   e-117

>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
            somniferum]
          Length = 351

 Score =  688 bits (1776), Expect = 0.0
 Identities = 337/351 (96%), Positives = 345/351 (98%)
 Frame = +3

Query: 435  MQLMAKEELLRNMELGLIPDQEIRQRIRIELEKRLQWGYKETHEEQLSQLLELVHSLRGM 614
            MQL AKEELLRNMELGLIPDQEIRQ IR+ELEKRLQWGYKETHEEQLSQLL+LVHSL+GM
Sbjct: 1    MQLKAKEELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGM 60

Query: 615  KMATEMENLDLKLYEAPMEFLKIQHGRNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQI 794
            KMATEMENLDLKLYEAPMEFLKIQHG NMKQSAGYYTDESTTLDEAEIAMLDLYMERAQI
Sbjct: 61   KMATEMENLDLKLYEAPMEFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQI 120

Query: 795  KDGQSVLDLGCGLGAVALFGANKFKNCQFTGVTSSMEQKDYIERKCKELKLTNVKVLLAD 974
            KDGQSVLDLGCGLGAVALFGANKFK CQFTGVTSS+EQKDYIE KCKELKLTNVKVLLAD
Sbjct: 121  KDGQSVLDLGCGLGAVALFGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLAD 180

Query: 975  ITTYETEERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVD 1154
            ITTYETEERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVD
Sbjct: 181  ITTYETEERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVD 240

Query: 1155 AEDWYTNYVFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVK 1334
            AEDWYTNY+FPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIV+
Sbjct: 241  AEDWYTNYIFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVE 300

Query: 1335 FKEIMKSITKTEEEANKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 1487
            FKEIM+SITKTE+EA KLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK
Sbjct: 301  FKEIMRSITKTEKEAIKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 351


>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  469 bits (1207), Expect = e-130
 Identities = 218/344 (63%), Positives = 276/344 (80%)
 Frame = +3

Query: 456  ELLRNMELGLIPDQEIRQRIRIELEKRLQWGYKETHEEQLSQLLELVHSLRGMKMATEME 635
            ELL+ +ELGL+P  +I+Q IR EL +RLQWGYK T+EEQ++++  L HSLR MK+ATE+E
Sbjct: 15   ELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNLTHSLRQMKIATEVE 74

Query: 636  NLDLKLYEAPMEFLKIQHGRNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVL 815
             LD +LYE P+EFLKI +G N+K S  Y+ ++STTLDEAEIAMLDLY ERAQI+DGQSVL
Sbjct: 75   TLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVL 134

Query: 816  DLGCGLGAVALFGANKFKNCQFTGVTSSMEQKDYIERKCKELKLTNVKVLLADITTYETE 995
            DLGCG GA+ L  A K+KNC+ T VT+S+ QK+YIE + +   L NV+V LADITT+E  
Sbjct: 135  DLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMA 194

Query: 996  ERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTN 1175
            E +DRI  +EL EHMKNY+LLL+KISEW+  DGLLF+EH+CHKT AYHYEP+D +DW+T 
Sbjct: 195  ETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTE 254

Query: 1176 YVFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVKFKEIMKS 1355
            YVFPAGT+ + SAS  LYFQDDVSVVN WTLSGKH+SR++EEWLK +D N+   K + ++
Sbjct: 255  YVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFET 314

Query: 1356 ITKTEEEANKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 1487
            +   EEEA KL+N+WR FC+ G E+FGY NGEEWM +H+LFKKK
Sbjct: 315  LMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 358


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
            gi|51980204|gb|AAU20766.1| (S)-coclaurine
            N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  464 bits (1194), Expect = e-128
 Identities = 216/345 (62%), Positives = 279/345 (80%), Gaps = 1/345 (0%)
 Frame = +3

Query: 456  ELLRNMELGLIPDQEIRQRIRIELEKRLQWGYKETHEEQLSQLLELVHSLRGMKMATEME 635
            ELL+ +ELGL+PD EI++ IRI+L +RLQWG K T+EEQ++QL+ L HSLR MK+ATE+E
Sbjct: 17   ELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLVNLTHSLRQMKIATEVE 76

Query: 636  NLDLKLYEAPMEFLKIQHGRNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVL 815
             LD ++YE P++FLKI +G N+K S  Y+ ++STTLDEAEIAML+LY ERAQIKDG SVL
Sbjct: 77   TLDDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAMLELYCERAQIKDGHSVL 136

Query: 816  DLGCGLGAVALFGANKFKNCQFTGVTSSMEQKDYIERKCKELKLTNVKVLLADITTYETE 995
            DLGCG GA+ L+ A K+KN + T VT+S+ QK++IE + ++  L+NV+VLLADITT++  
Sbjct: 137  DLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNLSNVEVLLADITTHKMP 196

Query: 996  ERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTN 1175
            + +DRI  VEL EHMKNY+LLL+KI EWM  DGLLFVEH+CHKT AYHYEP+D +DW+T 
Sbjct: 197  DTYDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPIDEDDWFTE 256

Query: 1176 YVFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVKFKEIMKS 1355
            YVFPAGT+ + SAS  LYFQDDVSVVN WTLSGKH+SR++EEWLK +D N+   K +  +
Sbjct: 257  YVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANVELIKPMFVT 316

Query: 1356 IT-KTEEEANKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 1487
            IT +  +EA KL+N+WR FC+ G E+FGY NGEEWM +H+LFKKK
Sbjct: 317  ITGQCRQEAMKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 361


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  442 bits (1138), Expect = e-122
 Identities = 206/346 (59%), Positives = 266/346 (76%)
 Frame = +3

Query: 450  KEELLRNMELGLIPDQEIRQRIRIELEKRLQWGYKETHEEQLSQLLELVHSLRGMKMATE 629
            K+EL + +ELGLIP+ E+++ +RI+L +RLQWGYK T+EEQ++Q+L+L HSLR M +A E
Sbjct: 6    KQELFKKLELGLIPNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAME 65

Query: 630  MENLDLKLYEAPMEFLKIQHGRNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQS 809
            ++ LD  +YE P++FLKI +G  +K S+ Y+ D+STTLDEAEIAM+DL+ ERAQIKDG S
Sbjct: 66   VDTLDSNMYEVPLDFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKDGHS 125

Query: 810  VLDLGCGLGAVALFGANKFKNCQFTGVTSSMEQKDYIERKCKELKLTNVKVLLADITTYE 989
            VLDLGCG G++  + A K+K    T VT+S+ QK+YIE + +   L+NV+VLLADI T+E
Sbjct: 126  VLDLGCGHGSLTFYVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIATHE 185

Query: 990  TEERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWY 1169
              + +DRI  V L EHMKNY LLLKKISEWM  DGLLFV+HVCHKT A+H EP+D +DW 
Sbjct: 186  MADTYDRILVVGLFEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWL 245

Query: 1170 TNYVFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVKFKEIM 1349
            + Y FPAGT+ + SAS  LYFQDD SVVN WTLSGKH+SR+ EEWLK +D N+     + 
Sbjct: 246  SEYAFPAGTMIIPSASFFLYFQDDASVVNHWTLSGKHFSRTTEEWLKRLDANVKVIIPMF 305

Query: 1350 KSITKTEEEANKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 1487
             +IT +EEEA KLLN+WR  C  G EL+GY NGEEWM +H+LFKKK
Sbjct: 306  ATITGSEEEAVKLLNYWRGLCFFGVELYGYNNGEEWMESHVLFKKK 351


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
            gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
            [Thalictrum flavum]
          Length = 356

 Score =  428 bits (1100), Expect = e-117
 Identities = 194/347 (55%), Positives = 269/347 (77%)
 Frame = +3

Query: 447  AKEELLRNMELGLIPDQEIRQRIRIELEKRLQWGYKETHEEQLSQLLELVHSLRGMKMAT 626
            A   L++ +E G + D+EIR  ++I+++KRL+WGYK THE+QL+QL+    SL+GM+MA 
Sbjct: 10   AVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGMEMAE 69

Query: 627  EMENLDLKLYEAPMEFLKIQHGRNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQ 806
            E++ LD +LYE P+ FL I  G+ +K S GY+ DESTTLDE+E+ M+DLY ERAQIKDGQ
Sbjct: 70   EVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQIKDGQ 129

Query: 807  SVLDLGCGLGAVALFGANKFKNCQFTGVTSSMEQKDYIERKCKELKLTNVKVLLADITTY 986
            S+LDLGCG G++ L  A K++ C+ TG+T+S+ QK++I  +CK+L L+NV+++L D+T +
Sbjct: 130  SILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDVTKF 189

Query: 987  ETEERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDW 1166
            ETE  +DRIFAV LIEHMKNY+L LKK+S W+   GLLFVEH CHK  AY YEP+D +DW
Sbjct: 190  ETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQYEPLDEDDW 249

Query: 1167 YTNYVFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVKFKEI 1346
            YT Y+FP+GTL +SS+S+LLYFQ+DVSVVN WTLSGKH S   ++WLK +D NI + KEI
Sbjct: 250  YTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEI 309

Query: 1347 MKSITKTEEEANKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 1487
             +S   ++E+A K + +WR+FC+  ++++   NGEEWML+ +LFKKK
Sbjct: 310  FESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQVLFKKK 356