BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver23_contig00006632
         (1586 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]             416   e-114
gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]   416   e-113
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   286   1e-74
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   278   4e-72
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...   276   1e-71

>gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]
          Length = 356

 Score =  416 bits (1069), Expect = e-114
 Identities = 213/360 (59%), Positives = 269/360 (74%), Gaps = 3/360 (0%)
 Frame = -3

Query: 1500 MEIQMEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSL 1321
            MEI +E +E EMKYQSQIWN +CG+V+TSVL+C+I LGIFDAIH+SGK M+TLTELS+ +
Sbjct: 1    MEIHLESQEQEMKYQSQIWNQICGTVDTSVLRCAIQLGIFDAIHNSGKPMITLTELSSIV 60

Query: 1320 SF--SNMIKTHNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIG 1147
            S   S+ I+  NLYRL+RYLS M+LI+I         SLT  SKLLL NQEKSL DW + 
Sbjct: 61   SSPSSSSIEPCNLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLA 120

Query: 1146 IGDVNFVATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLA 967
            I     V  WHE+SSS S    +   ++ VHGK   EL  E PE+++  + AM+SDT++ 
Sbjct: 121  ISCEMMVVVWHELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVT 180

Query: 966  MPAFIQGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCH 787
             PA IQGC  I++G++SL+DVGGG G  M Y+V+AFPHIK  V D+PHV+  A +     
Sbjct: 181  KPALIQGCGKILNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERP--- 237

Query: 786  GIEFIGGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDE 607
            G+EFI GD+F  I  ADA+LLK++LHNWED EC N+LKRCKEA+PADKGKVIIMD+V+D+
Sbjct: 238  GVEFISGDIFKSISNADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVIDD 297

Query: 606  NADNDDLVQAKLSMDLDMMS-SGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 430
            + DN  L QAKLS+DL +M+  GGRERTKEDW+NL+EMSGF+ HEIIPI A+ SIIVAYP
Sbjct: 298  D-DNSILTQAKLSLDLTVMNHGGGRERTKEDWRNLIEMSGFSRHEIIPISAMPSIIVAYP 356


>gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]
          Length = 356

 Score =  416 bits (1068), Expect = e-113
 Identities = 213/360 (59%), Positives = 269/360 (74%), Gaps = 3/360 (0%)
 Frame = -3

Query: 1500 MEIQMEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSL 1321
            MEI +E +E EMKYQSQIWN +CG+V+TSVL+C+I LGIFDAIH+SGK M+TLTELS+ +
Sbjct: 1    MEIHLESQEQEMKYQSQIWNQICGTVDTSVLRCAIQLGIFDAIHNSGKPMITLTELSSIV 60

Query: 1320 SF--SNMIKTHNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIG 1147
            S   S+ I+  NLYRL+RYLS M+LI+I         SLT  SKLLL NQEKSL DW + 
Sbjct: 61   SSPSSSSIEPCNLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLA 120

Query: 1146 IGDVNFVATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLA 967
            I     V  WHE+SSS S    +   ++ VHGK   EL  E PE+++  + AM+SDT++ 
Sbjct: 121  IXCEMMVVVWHELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVT 180

Query: 966  MPAFIQGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCH 787
             PA IQGC  I++G++SL+DVGGG G  M Y+V+AFPHIK  V D+PHV+  A +     
Sbjct: 181  KPALIQGCGKILNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERP--- 237

Query: 786  GIEFIGGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDE 607
            G+EFI GD+F  I  ADA+LLK++LHNWED EC N+LKRCKEA+PADKGKVIIMD+V+D+
Sbjct: 238  GVEFISGDIFKSISNADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVIDD 297

Query: 606  NADNDDLVQAKLSMDLDMMS-SGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 430
            + DN  L QAKLS+DL +M+  GGRERTKEDW+NL+EMSGF+ HEIIPI A+ SIIVAYP
Sbjct: 298  D-DNSILTQAKLSLDLTVMNHGGGRERTKEDWRNLIEMSGFSRHEIIPISAMPSIIVAYP 356


>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase; Short=6-OMT
            gi|9711914|dbj|BAB08004.1|
            S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  286 bits (731), Expect = 1e-74
 Identities = 153/354 (43%), Positives = 229/354 (64%), Gaps = 1/354 (0%)
 Frame = -3

Query: 1488 MEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSN 1309
            ME ++  +  Q+++WN + G   + VLKC++ L + + IH+SG SM TL+ELS+ L  S 
Sbjct: 1    MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSM-TLSELSSRLP-SQ 58

Query: 1308 MIKTHNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNF 1129
             +    LYR++RYL HM L   +SI G   + L   +K L+   +K +    + I D +F
Sbjct: 59   PVNEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDF 118

Query: 1128 VATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQ 949
            +A WH +    S   G  TA+E   G   +    E PE ++ F++AM++D+ L M A ++
Sbjct: 119  MAPWHYLKDGLSGESG--TAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVK 176

Query: 948  GCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIG 769
             C  I +GI++LVDVGGG GTA+  +  AFPHIKCTV+D+PHVI   AD      +  + 
Sbjct: 177  ECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVI---ADSPGYSEVHCVA 233

Query: 768  GDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDD 589
            GD+F FIP ADAI++K +LH+W+D+EC  ILKRCKEA+P   GKVII+DIVL+  +++  
Sbjct: 234  GDMFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEH-P 292

Query: 588  LVQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 430
              + +L++DLDMM ++GG+ERT+E+WK L+  +G+  H+I  I A+QS+I AYP
Sbjct: 293  YTKMRLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase 2 [Papaver somniferum]
          Length = 357

 Score =  278 bits (710), Expect = 4e-72
 Identities = 145/353 (41%), Positives = 229/353 (64%), Gaps = 3/353 (0%)
 Frame = -3

Query: 1479 EELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIK 1300
            +E+ +K Q+Q+WN + G  ++ VL+C++ +GI D I ++  + +TL +L+  L  +N + 
Sbjct: 13   QEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGA-ITLAQLAAKLPITN-VS 70

Query: 1299 THNLYRLLRYLSHMNLIAISSIQGNDH--FSLTDLSKLLLGNQEKSLKDWAIGIGDVNFV 1126
            +  LYR++RYL H+N+I   +  G     +SL  +  LLL + E+S+    +G+   +F+
Sbjct: 71   SDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFM 130

Query: 1125 ATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQG 946
             +WH M        G  TA+E   G + ++    +P+ S+ F++ M+ +T L     I+ 
Sbjct: 131  VSWHFMKEGLGN--GSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIED 188

Query: 945  CDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGG 766
            C     G+ SLVD+GGG GT +  + +AFPHIKCT++D+PHV+  + D      IE + G
Sbjct: 189  CRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHD---LPNIEKVPG 245

Query: 765  DLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDL 586
            D+F  +P A AILLK +LH+W DEEC NILK+CKEAIP + GKVII+D+ L+E + N +L
Sbjct: 246  DMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEES-NHEL 304

Query: 585  VQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 430
             + +L +D+DM+ ++GGRERT +DW+NLL+ +GF SH+I PI AIQS+I A+P
Sbjct: 305  TKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
            glaucum]
          Length = 350

 Score =  276 bits (705), Expect = 1e-71
 Identities = 147/344 (42%), Positives = 220/344 (63%), Gaps = 1/344 (0%)
 Frame = -3

Query: 1458 QSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIKTHNLYRL 1279
            Q+++WN + G  ++ VLK ++ L + + IH+ G  M TL+ELS  L  S  +    LYR+
Sbjct: 12   QAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPM-TLSELSLHLP-SQPVNQDALYRV 69

Query: 1278 LRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNFVATWHEMSSS 1099
            LRYL HM L   SSI G   + L   +K L+   +K +    + I D +F+A WH +   
Sbjct: 70   LRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPWHYLKEG 129

Query: 1098 CSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQGCDIIMSGIS 919
                    TA+E   G   ++   E PE ++ F++ M++DT L M A ++ C  +  GI+
Sbjct: 130  ILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSSMFDGIT 189

Query: 918  SLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGGDLFSFIPPA 739
            ++VDVGGG GTA+  + KAFPHIKCTV+D+PHVI   AD      I  I GD+F +IP A
Sbjct: 190  TIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVI---ADSPGYTEINSIQGDMFKYIPNA 246

Query: 738  DAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDLVQAKLSMDL 559
            DAI++K +LH+W+D+EC  ILKRCK+A+P D GKVII+DI+LD  +++    + +L++DL
Sbjct: 247  DAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEH-PYTKMRLTLDL 305

Query: 558  DMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 430
            DMM ++GG+ERT+E+WK L+  +G+  ++I  I A+QS+I AYP
Sbjct: 306  DMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYP 349