BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver23_contig00006450
         (835 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFK73712.1| pavine-N-methyltransferase [Papaver somniferum]        204   2e-50
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      145   8e-33
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   144   2e-32
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   138   1e-30
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       136   5e-30

>gb|AFK73712.1| pavine-N-methyltransferase [Papaver somniferum]
          Length = 330

 Score =  204 bits (519), Expect = 2e-50
 Identities = 94/100 (94%), Positives = 96/100 (96%)
 Frame = -1

Query: 301 DRVFVIGLIEHMKNFELFLRKISKWMKEDGLLFLEHLCHKSFSDHWEPLSEDDWYANNFF 122
           DRVFVIGLIEHMKNFELFLRKISKWMK+DGLL LEHLCHKSFSDHWEPLSEDDWYA NFF
Sbjct: 164 DRVFVIGLIEHMKNFELFLRKISKWMKDDGLLLLEHLCHKSFSDHWEPLSEDDWYAKNFF 223

Query: 121 PSGTLVIPSATCLLYFQEDVTVKDHWLLSGNNFARSNEAI 2
           PSGTLVIPSATCLLYFQEDVTV DHW+LSGNNFARSNE I
Sbjct: 224 PSGTLVIPSATCLLYFQEDVTVIDHWILSGNNFARSNEVI 263



 Score =  179 bits (455), Expect = 5e-43
 Identities = 93/137 (67%), Positives = 100/137 (72%)
 Frame = -2

Query: 834 GMKMATEVHALENHKIYETPDSFNQIIGGKESAGLFTDETTTTIEEANIKMMDLYCXXXX 655
           GMKMATE+  LENHKIYETP+SFNQIIGGKESAGLFTDETTTT+EEAN KMMD       
Sbjct: 33  GMKMATEIDTLENHKIYETPESFNQIIGGKESAGLFTDETTTTMEEANTKMMD------- 85

Query: 654 XXXXXXXXXXXXXXXXXXXXXXLYCERAGLKDGQTILDIGCGAGLLVLHLAKKYKNCKIT 475
                                 LYCERAGLKDG TILD+GCGAGLLVLHLAKKYK  KIT
Sbjct: 86  ----------------------LYCERAGLKDGHTILDLGCGAGLLVLHLAKKYKKSKIT 123

Query: 474 GVTSTLWHKEYILEQCK 424
           G+T+T  HKEYIL+QCK
Sbjct: 124 GITNTSSHKEYILKQCK 140


>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  145 bits (367), Expect = 8e-33
 Identities = 61/98 (62%), Positives = 82/98 (83%)
 Frame = -1

Query: 301 DRVFVIGLIEHMKNFELFLRKISKWMKEDGLLFLEHLCHKSFSDHWEPLSEDDWYANNFF 122
           DR+ VI L EHMKN+EL LRKIS+W+ +DGLLFLEH+CHK+F+ H+EPL +DDW+    F
Sbjct: 198 DRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVF 257

Query: 121 PSGTLVIPSATCLLYFQEDVTVKDHWLLSGNNFARSNE 8
           P+GT++IPSA+  LYFQ+DV+V +HW LSG +F+R+NE
Sbjct: 258 PAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNE 295



 Score = 97.8 bits (242), Expect = 3e-18
 Identities = 54/138 (39%), Positives = 80/138 (57%), Gaps = 2/138 (1%)
 Frame = -2

Query: 831 MKMATEVHALENHKIYETPDSFNQIIGGKESAG--LFTDETTTTIEEANIKMMDLYCXXX 658
           MK+ATEV  L++ ++YE P  F +I+ G    G   +  E +TT++EA I M+DLYC   
Sbjct: 67  MKIATEVETLDS-QLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYC--- 122

Query: 657 XXXXXXXXXXXXXXXXXXXXXXXLYCERAGLKDGQTILDIGCGAGLLVLHLAKKYKNCKI 478
                                     ERA ++DGQ++LD+GCG G L LH+A+KYKNC++
Sbjct: 123 --------------------------ERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRV 156

Query: 477 TGVTSTLWHKEYILEQCK 424
           T VT+++  KEYI E+ +
Sbjct: 157 TAVTNSVSQKEYIEEESR 174


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
           gi|51980204|gb|AAU20766.1| (S)-coclaurine
           N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  144 bits (363), Expect = 2e-32
 Identities = 59/98 (60%), Positives = 81/98 (82%)
 Frame = -1

Query: 301 DRVFVIGLIEHMKNFELFLRKISKWMKEDGLLFLEHLCHKSFSDHWEPLSEDDWYANNFF 122
           DR+ V+ L EHMKN+EL LRKI +WM +DGLLF+EH+CHK+F+ H+EP+ EDDW+    F
Sbjct: 200 DRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPIDEDDWFTEYVF 259

Query: 121 PSGTLVIPSATCLLYFQEDVTVKDHWLLSGNNFARSNE 8
           P+GT++IPSA+  LYFQ+DV+V +HW LSG +F+R+NE
Sbjct: 260 PAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNE 297



 Score = 86.3 bits (212), Expect = 8e-15
 Identities = 49/138 (35%), Positives = 77/138 (55%), Gaps = 2/138 (1%)
 Frame = -2

Query: 831 MKMATEVHALENHKIYETPDSFNQIIGGKESAG--LFTDETTTTIEEANIKMMDLYCXXX 658
           MK+ATEV  L++ ++YE P  F +I+ G    G   +    +TT++EA I M++LYC   
Sbjct: 69  MKIATEVETLDD-QMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAMLELYC--- 124

Query: 657 XXXXXXXXXXXXXXXXXXXXXXXLYCERAGLKDGQTILDIGCGAGLLVLHLAKKYKNCKI 478
                                     ERA +KDG ++LD+GCG G L L++A+KYKN ++
Sbjct: 125 --------------------------ERAQIKDGHSVLDLGCGQGALTLYVAQKYKNSRV 158

Query: 477 TGVTSTLWHKEYILEQCK 424
           T VT+++  KE+I E+ +
Sbjct: 159 TAVTNSVSQKEFIEEESR 176


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
           somniferum]
          Length = 351

 Score =  138 bits (348), Expect = 1e-30
 Identities = 57/98 (58%), Positives = 81/98 (82%)
 Frame = -1

Query: 301 DRVFVIGLIEHMKNFELFLRKISKWMKEDGLLFLEHLCHKSFSDHWEPLSEDDWYANNFF 122
           DR+F + LIEHMKN++L L+KIS+WMK+DGLLF+EH+CHK+ + H+EP+  +DWY N  F
Sbjct: 191 DRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTNYIF 250

Query: 121 PSGTLVIPSATCLLYFQEDVTVKDHWLLSGNNFARSNE 8
           P+GTL + SA+ LLYFQ+DV+V + W LSG +++RS+E
Sbjct: 251 PAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHE 288



 Score = 95.1 bits (235), Expect = 2e-17
 Identities = 59/140 (42%), Positives = 80/140 (57%), Gaps = 3/140 (2%)
 Frame = -2

Query: 834 GMKMATEVHALENHKIYETPDSFNQIIGG---KESAGLFTDETTTTIEEANIKMMDLYCX 664
           GMKMATE+  L+  K+YE P  F +I  G   K+SAG +TDE+TT ++EA I M+DLY  
Sbjct: 59  GMKMATEMENLDL-KLYEAPMEFLKIQHGSNMKQSAGYYTDESTT-LDEAEIAMLDLYM- 115

Query: 663 XXXXXXXXXXXXXXXXXXXXXXXXXLYCERAGLKDGQTILDIGCGAGLLVLHLAKKYKNC 484
                                       ERA +KDGQ++LD+GCG G + L  A K+K C
Sbjct: 116 ----------------------------ERAQIKDGQSVLDLGCGLGAVALFGANKFKKC 147

Query: 483 KITGVTSTLWHKEYILEQCK 424
           + TGVTS++  K+YI  +CK
Sbjct: 148 QFTGVTSSVEQKDYIEGKCK 167


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  136 bits (343), Expect = 5e-30
 Identities = 56/98 (57%), Positives = 79/98 (80%)
 Frame = -1

Query: 301 DRVFVIGLIEHMKNFELFLRKISKWMKEDGLLFLEHLCHKSFSDHWEPLSEDDWYANNFF 122
           DR+ V+GL EHMKN+ L L+KIS+WM +DGLLF++H+CHK+F+ H EP+ EDDW +   F
Sbjct: 191 DRILVVGLFEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSEYAF 250

Query: 121 PSGTLVIPSATCLLYFQEDVTVKDHWLLSGNNFARSNE 8
           P+GT++IPSA+  LYFQ+D +V +HW LSG +F+R+ E
Sbjct: 251 PAGTMIIPSASFFLYFQDDASVVNHWTLSGKHFSRTTE 288



 Score = 79.3 bits (194), Expect = 1e-12
 Identities = 50/139 (35%), Positives = 76/139 (54%), Gaps = 3/139 (2%)
 Frame = -2

Query: 831 MKMATEVHALENHKIYETPDSFNQIIGG---KESAGLFTDETTTTIEEANIKMMDLYCXX 661
           M +A EV  L+++ +YE P  F +I+ G   K S+  F D++TT ++EA I MMDL+C  
Sbjct: 60  MNIAMEVDTLDSN-MYEVPLDFLKIMNGSALKMSSCYFKDDSTT-LDEAEIAMMDLHC-- 115

Query: 660 XXXXXXXXXXXXXXXXXXXXXXXXLYCERAGLKDGQTILDIGCGAGLLVLHLAKKYKNCK 481
                                      ERA +KDG ++LD+GCG G L  ++A+KYK   
Sbjct: 116 ---------------------------ERAQIKDGHSVLDLGCGHGSLTFYVAQKYKKSH 148

Query: 480 ITGVTSTLWHKEYILEQCK 424
           +T VT+++  KEYI E+ +
Sbjct: 149 VTAVTNSVSQKEYIEEEAR 167