BLASTX nr result
ID: Paeonia25_contig00027760
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Paeonia25_contig00027760
(417 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EMD33736.1| glycoside hydrolase family 18 protein [Ceriporiop... 168 8e-40
ref|XP_007386178.1| chitinase [Punctularia strigosozonata HHB-11... 158 8e-37
gb|EPQ57125.1| chitinase [Gloeophyllum trabeum ATCC 11539] 157 2e-36
ref|XP_007361831.1| chitinase [Dichomitus squalens LYAD-421 SS1]... 152 4e-35
gb|EMD39774.1| glycoside hydrolase family 18 protein [Ceriporiop... 150 2e-34
gb|EIW51657.1| glycoside hydrolase [Trametes versicolor FP-10166... 149 4e-34
gb|EMD34757.1| glycoside hydrolase family 18 protein [Ceriporiop... 148 7e-34
gb|EIW62947.1| chitinase [Trametes versicolor FP-101664 SS1] 146 3e-33
gb|ABB90389.1| chitinase [Taiwanofungus camphoratus] 144 1e-32
gb|ETW75803.1| glycoside hydrolase family 18 protein [Heterobasi... 143 2e-32
ref|XP_002469929.1| hypothetical protein POSPLDRAFT_134923 [Post... 142 4e-32
ref|XP_006456795.1| hypothetical protein AGABI2DRAFT_211936 [Aga... 142 5e-32
ref|XP_007328970.1| hypothetical protein AGABI1DRAFT_72570 [Agar... 142 5e-32
emb|CAQ51152.1| chitinase [Coprinellus congregatus] 142 6e-32
emb|CCM03883.1| predicted protein [Fibroporia radiculosa] 140 1e-31
ref|XP_007313460.1| glycoside hydrolase family 18 protein [Serpu... 140 1e-31
gb|EIW77028.1| glycoside hydrolase family 18 protein [Coniophora... 140 2e-31
gb|ETW81104.1| glycoside hydrolase family 18 protein [Heterobasi... 140 2e-31
ref|XP_002469694.1| hypothetical protein POSPLDRAFT_114058 [Post... 138 9e-31
ref|XP_001837403.1| endochitinase [Coprinopsis cinerea okayama7#... 138 9e-31
>gb|EMD33736.1| glycoside hydrolase family 18 protein [Ceriporiopsis subvermispora
B]
Length = 1037
Score = 168 bits (425), Expect = 8e-40
Identities = 79/105 (75%), Positives = 89/105 (84%)
Frame = +1
Query: 1 PAGIDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMV 180
P D+CG S G+FDFWGLIDAGFL A+G+ A GIDY FD CS+TAYVYNETS VMV
Sbjct: 322 PTNTDQCGQTSGPSGVFDFWGLIDAGFLFANGSVAEGIDYRFDECSQTAYVYNETSEVMV 381
Query: 181 SFDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
SFDDA AFAAKG+++KSS LRGF+MWEAGGDS+DILLDSIRAAAG
Sbjct: 382 SFDDAKAFAAKGDFIKSSNLRGFAMWEAGGDSDDILLDSIRAAAG 426
>ref|XP_007386178.1| chitinase [Punctularia strigosozonata HHB-11173 SS5]
gi|390597297|gb|EIN06697.1| chitinase [Punctularia
strigosozonata HHB-11173 SS5]
Length = 410
Score = 158 bits (399), Expect = 8e-37
Identities = 73/105 (69%), Positives = 85/105 (80%)
Frame = +1
Query: 4 AGIDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVS 183
AG DECGN GGLFDFWGL+ AGFLT+ G APGI Y +D C++T YVYN TS VMVS
Sbjct: 306 AGNDECGNFESQGGLFDFWGLVQAGFLTSQGNPAPGISYRYDTCTQTPYVYNATSEVMVS 365
Query: 184 FDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAGL 318
FD+A +FA KG+++KSSGL GF+MWEAGGD +DILLDSIR AAGL
Sbjct: 366 FDNAQSFAQKGKFIKSSGLAGFAMWEAGGDFHDILLDSIRGAAGL 410
>gb|EPQ57125.1| chitinase [Gloeophyllum trabeum ATCC 11539]
Length = 412
Score = 157 bits (396), Expect = 2e-36
Identities = 69/102 (67%), Positives = 86/102 (84%)
Frame = +1
Query: 1 PAGIDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMV 180
PAGIDECGN VGGLFDFWGLI AGFLT+ G APGI + +D C+KT YVY+ TS +M+
Sbjct: 307 PAGIDECGNDEAVGGLFDFWGLIQAGFLTSEGNRAPGIIFRYDDCTKTPYVYDATSEIMI 366
Query: 181 SFDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRA 306
+FD+AP+F KG +++S+GLRGFSMWEAGGDSNDILLD+I++
Sbjct: 367 AFDNAPSFREKGTFIRSAGLRGFSMWEAGGDSNDILLDAIQS 408
>ref|XP_007361831.1| chitinase [Dichomitus squalens LYAD-421 SS1]
gi|395332817|gb|EJF65195.1| chitinase [Dichomitus
squalens LYAD-421 SS1]
Length = 429
Score = 152 bits (385), Expect = 4e-35
Identities = 72/104 (69%), Positives = 83/104 (79%)
Frame = +1
Query: 4 AGIDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVS 183
AG+DECGN S GG FDFWGLIDAGFLT SG AP Y+FD CS+TAYVY+E S V VS
Sbjct: 324 AGVDECGNESGPGGNFDFWGLIDAGFLTLSGDPAPLTAYNFDVCSQTAYVYHEESEVFVS 383
Query: 184 FDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
FDDA AF AKG ++K + LRGF++WEAGGD NDILLD+IR+ AG
Sbjct: 384 FDDARAFTAKGHFIKDTKLRGFAIWEAGGDFNDILLDAIRSGAG 427
>gb|EMD39774.1| glycoside hydrolase family 18 protein [Ceriporiopsis subvermispora
B]
Length = 418
Score = 150 bits (378), Expect = 2e-34
Identities = 70/100 (70%), Positives = 83/100 (83%)
Frame = +1
Query: 10 IDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVSFD 189
+D+CG S G+FDF+GLID GFL G+ A GIDY FD+CS+TAYVYN TS+VMVSFD
Sbjct: 317 VDQCGAVSGPSGVFDFFGLIDGGFLNPDGSVASGIDYRFDSCSQTAYVYNATSSVMVSFD 376
Query: 190 DAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAA 309
+A AFAAKG ++KSS LRGF++WEAGGDSNDILLDSIR A
Sbjct: 377 NAQAFAAKGNFIKSSNLRGFAIWEAGGDSNDILLDSIRKA 416
>gb|EIW51657.1| glycoside hydrolase [Trametes versicolor FP-101664 SS1]
Length = 436
Score = 149 bits (376), Expect = 4e-34
Identities = 71/106 (66%), Positives = 84/106 (79%), Gaps = 2/106 (1%)
Frame = +1
Query: 7 GIDECGNASPVG--GLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMV 180
G D+CG S G G+FDFWGLI+ GFLTA+GT A GIDY FDACS+T YVYN TS VMV
Sbjct: 326 GPDQCGVQSAGGPSGIFDFWGLIEGGFLTANGTVADGIDYRFDACSQTPYVYNPTSGVMV 385
Query: 181 SFDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAGL 318
S+DDA +FAAKG ++ +GLRGF+MWEA GDSNDILLD+I A G+
Sbjct: 386 SYDDAESFAAKGRFINEAGLRGFAMWEAAGDSNDILLDAITNAIGI 431
>gb|EMD34757.1| glycoside hydrolase family 18 protein [Ceriporiopsis subvermispora
B]
Length = 437
Score = 148 bits (374), Expect = 7e-34
Identities = 68/108 (62%), Positives = 86/108 (79%), Gaps = 2/108 (1%)
Frame = +1
Query: 1 PAGIDECGNASPVG--GLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNV 174
PAG D+CGN + G G+FDFWGL+ GFLT +GTAA GID+ +D CS+T YVYN + V
Sbjct: 325 PAGYDQCGNYTSGGPSGIFDFWGLVQGGFLTNNGTAASGIDFRYDNCSQTPYVYNPNTGV 384
Query: 175 MVSFDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAGL 318
MVS+DDA +FAAKG+++ S+GL+GF+MWEAGGD NDILLDSI A G+
Sbjct: 385 MVSYDDAQSFAAKGQFINSAGLKGFAMWEAGGDFNDILLDSITNAIGM 432
>gb|EIW62947.1| chitinase [Trametes versicolor FP-101664 SS1]
Length = 429
Score = 146 bits (369), Expect = 3e-33
Identities = 73/107 (68%), Positives = 86/107 (80%), Gaps = 2/107 (1%)
Frame = +1
Query: 1 PAGIDE-CGNASPV-GGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNV 174
PAG D+ CG + GG DFWGLIDA FL A+GTAAPGI+Y FD CS+T +VYNE + V
Sbjct: 322 PAGSDDGCGVLTTTPGGTMDFWGLIDAKFLNANGTAAPGINYRFDDCSQTPFVYNEATQV 381
Query: 175 MVSFDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
MVSFDDA +FA+KG+++KS LRGFSMWEAGGDS DILL+SIR AAG
Sbjct: 382 MVSFDDAHSFASKGDFIKSQKLRGFSMWEAGGDSADILLNSIRTAAG 428
>gb|ABB90389.1| chitinase [Taiwanofungus camphoratus]
Length = 429
Score = 144 bits (363), Expect = 1e-32
Identities = 66/102 (64%), Positives = 83/102 (81%)
Frame = +1
Query: 13 DECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVSFDD 192
D CG S G+FDFWGLID GFL +G+ + GI YS+D CS+T YVYN+ ++VMVSFD+
Sbjct: 320 DSCGVTSGPSGVFDFWGLIDGGFLYPNGSVSEGIAYSYDECSQTPYVYNQCTSVMVSFDN 379
Query: 193 APAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAGL 318
A +FAAKG+Y+K++ LRGF+MWEAGGD +DILLDSIR AAGL
Sbjct: 380 AESFAAKGDYIKNNNLRGFAMWEAGGDYDDILLDSIRQAAGL 421
>gb|ETW75803.1| glycoside hydrolase family 18 protein [Heterobasidion irregulare TC
32-1]
Length = 445
Score = 143 bits (361), Expect = 2e-32
Identities = 64/101 (63%), Positives = 79/101 (78%)
Frame = +1
Query: 13 DECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVSFDD 192
D+CGN +GG+FDFWGLI GFL A+GTA GI+Y FD CS+T YVYN + VMVS+DD
Sbjct: 341 DQCGNPPAIGGVFDFWGLIQGGFLNANGTAESGIEYRFDNCSQTPYVYNPNTQVMVSYDD 400
Query: 193 APAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
A +FAAKG ++ SGL GF+MWEAGGDSN+ILL++I A G
Sbjct: 401 ATSFAAKGTFISQSGLAGFAMWEAGGDSNNILLNAISTALG 441
>ref|XP_002469929.1| hypothetical protein POSPLDRAFT_134923 [Postia placenta Mad-698-R]
gi|220730991|gb|EED84840.1| hypothetical protein
POSPLDRAFT_134923 [Postia placenta Mad-698-R]
Length = 455
Score = 142 bits (359), Expect = 4e-32
Identities = 67/107 (62%), Positives = 83/107 (77%), Gaps = 3/107 (2%)
Frame = +1
Query: 4 AGIDECGNASPVGG---LFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNV 174
AG+D+CGN P GG +FDFWGL++ GFLT +GTAA GI Y FD CS+T YVYN + V
Sbjct: 348 AGVDQCGN--PTGGPSGIFDFWGLVEEGFLTDNGTAADGIAYRFDDCSQTPYVYNPDTKV 405
Query: 175 MVSFDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
MVSFDD +FAAKG ++ SGLRGF+MWE GGD D+L+D+IR+AAG
Sbjct: 406 MVSFDDPQSFAAKGSFISDSGLRGFAMWETGGDYEDMLVDAIRSAAG 452
>ref|XP_006456795.1| hypothetical protein AGABI2DRAFT_211936 [Agaricus bisporus var.
bisporus H97] gi|426192523|gb|EKV42459.1| hypothetical
protein AGABI2DRAFT_211936 [Agaricus bisporus var.
bisporus H97]
Length = 431
Score = 142 bits (358), Expect = 5e-32
Identities = 65/104 (62%), Positives = 81/104 (77%)
Frame = +1
Query: 4 AGIDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVS 183
AG DECG A GG DFWGLI+ G+LT +G A G+ + FD CS+T Y YN ++ +MV+
Sbjct: 326 AGNDECGVAQGPGGNIDFWGLIEQGYLTKNGDFAQGVPHRFDTCSQTPYAYNTSAEIMVA 385
Query: 184 FDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
FD+A +FAAKG++VKS G+RGF+MWEAGGDSNDILLDSIR A G
Sbjct: 386 FDNAQSFAAKGKFVKSKGMRGFAMWEAGGDSNDILLDSIRKAVG 429
>ref|XP_007328970.1| hypothetical protein AGABI1DRAFT_72570 [Agaricus bisporus var.
burnettii JB137-S8] gi|409079525|gb|EKM79886.1|
hypothetical protein AGABI1DRAFT_72570 [Agaricus
bisporus var. burnettii JB137-S8]
Length = 431
Score = 142 bits (358), Expect = 5e-32
Identities = 65/104 (62%), Positives = 82/104 (78%)
Frame = +1
Query: 4 AGIDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVS 183
AG DECG A GG DFWGLI+ G+LT +G A G+ + FD CS++ YVYN ++ +MV+
Sbjct: 326 AGNDECGVAQGPGGNIDFWGLIEQGYLTQNGDFAQGVPHRFDTCSQSPYVYNTSAEIMVA 385
Query: 184 FDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
FD+A +FAAKG++VKS G+RGF+MWEAGGDSNDILLDSIR A G
Sbjct: 386 FDNAQSFAAKGKFVKSKGMRGFAMWEAGGDSNDILLDSIRKAVG 429
>emb|CAQ51152.1| chitinase [Coprinellus congregatus]
Length = 441
Score = 142 bits (357), Expect = 6e-32
Identities = 64/104 (61%), Positives = 84/104 (80%)
Frame = +1
Query: 4 AGIDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVS 183
A ID CG +P GG F+FWGLI GFL GT APGI Y +D+CS+T YVYN T+ +MVS
Sbjct: 333 AAIDVCGVMNPPGGNFNFWGLIANGFLKRDGTPAPGIAYRYDSCSQTPYVYNATTEIMVS 392
Query: 184 FDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
+D+A +FAAKG +++S GL+GF+MWEAGGD+++ILLDSIR++AG
Sbjct: 393 YDNAQSFAAKGNFIRSLGLKGFAMWEAGGDTDNILLDSIRSSAG 436
>emb|CCM03883.1| predicted protein [Fibroporia radiculosa]
Length = 425
Score = 140 bits (354), Expect = 1e-31
Identities = 63/102 (61%), Positives = 82/102 (80%)
Frame = +1
Query: 10 IDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVSFD 189
ID CG + G+FDFWG+ID G+L +GT A G+DY +D CS+T YVYN ++VM+S+D
Sbjct: 319 IDACGVNTGPSGVFDFWGMIDGGYLFPNGTVAAGVDYRYDECSQTPYVYNCRTDVMISYD 378
Query: 190 DAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
+A +FAAKG+Y+ S+ LRGF+MWEAGGD NDILLDSIR+AAG
Sbjct: 379 NAESFAAKGKYIASTNLRGFAMWEAGGDYNDILLDSIRSAAG 420
>ref|XP_007313460.1| glycoside hydrolase family 18 protein [Serpula lacrymans var.
lacrymans S7.9] gi|336375123|gb|EGO03459.1| glycoside
hydrolase family 18 protein [Serpula lacrymans var.
lacrymans S7.3] gi|336388074|gb|EGO29218.1| glycoside
hydrolase family 18 protein [Serpula lacrymans var.
lacrymans S7.9]
Length = 443
Score = 140 bits (354), Expect = 1e-31
Identities = 67/105 (63%), Positives = 84/105 (80%), Gaps = 1/105 (0%)
Frame = +1
Query: 7 GIDECGNA-SPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVS 183
G D CG + GG ++F+G+I+ GFLTA+GTAAPGIDY FD CS+T YVYN TSNVM+S
Sbjct: 332 GSDVCGAPYTQPGGTYEFFGMIENGFLTANGTAAPGIDYRFDECSQTPYVYNSTSNVMIS 391
Query: 184 FDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAGL 318
+DDA +FAAKG Y+ +GLRGF+M+EA GDSNDILLD+I A G+
Sbjct: 392 YDDARSFAAKGAYINQAGLRGFAMFEAAGDSNDILLDAISNAIGI 436
>gb|EIW77028.1| glycoside hydrolase family 18 protein [Coniophora puteana
RWD-64-598 SS2]
Length = 449
Score = 140 bits (353), Expect = 2e-31
Identities = 62/104 (59%), Positives = 81/104 (77%)
Frame = +1
Query: 4 AGIDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVS 183
AG D CGNA+P GG ++FWGL+D GFL +GT A G Y +D C++T YVY+ TS VMV+
Sbjct: 344 AGTDVCGNATPAGGTYEFWGLVDNGFLNGNGTVAQGKLYRYDECTQTPYVYDPTSQVMVA 403
Query: 184 FDDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
+DDA +FAAKG Y+KS+GL+GF+++E GGD NDILLD+I A G
Sbjct: 404 YDDATSFAAKGAYIKSAGLKGFALYETGGDYNDILLDAITGAVG 447
>gb|ETW81104.1| glycoside hydrolase family 18 protein [Heterobasidion irregulare TC
32-1]
Length = 422
Score = 140 bits (352), Expect = 2e-31
Identities = 63/103 (61%), Positives = 83/103 (80%)
Frame = +1
Query: 7 GIDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVSF 186
G D CGNA GG+F+F+GL++ GFL +GT A GI Y+FD CS+T +VYN TS VMVS+
Sbjct: 318 GPDVCGNAQGAGGVFNFFGLVEGGFLNDNGTVAAGIAYAFDECSQTPFVYNSTSQVMVSY 377
Query: 187 DDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
D+A +FAAKG+++ + GLRGF+MWEAGGD DIL+DSIR+A+G
Sbjct: 378 DNADSFAAKGKFINTLGLRGFAMWEAGGDFKDILVDSIRSASG 420
>ref|XP_002469694.1| hypothetical protein POSPLDRAFT_114058 [Postia placenta Mad-698-R]
gi|220731311|gb|EED85157.1| hypothetical protein
POSPLDRAFT_114058 [Postia placenta Mad-698-R]
Length = 381
Score = 138 bits (347), Expect = 9e-31
Identities = 61/101 (60%), Positives = 79/101 (78%)
Frame = +1
Query: 13 DECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVSFDD 192
D CGN S G+FDFWG+ID GFL A+GT A IDY +D CS+T YVYN+++ VM+S+D+
Sbjct: 276 DVCGNTSGPSGVFDFWGMIDEGFLFANGTPAAAIDYRYDECSQTPYVYNKSTGVMISYDN 335
Query: 193 APAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
A +F AKG Y+ ++ LRGF+MWEAGGD +DILLD+IR AG
Sbjct: 336 AESFIAKGSYIANNNLRGFAMWEAGGDYDDILLDAIRQGAG 376
>ref|XP_001837403.1| endochitinase [Coprinopsis cinerea okayama7#130]
gi|116501424|gb|EAU84319.1| endochitinase [Coprinopsis
cinerea okayama7#130]
Length = 425
Score = 138 bits (347), Expect = 9e-31
Identities = 62/103 (60%), Positives = 78/103 (75%)
Frame = +1
Query: 7 GIDECGNASPVGGLFDFWGLIDAGFLTASGTAAPGIDYSFDACSKTAYVYNETSNVMVSF 186
G D CG+ + GG F+FWGLI+ GFL G+ GI Y +D CS+T YVYN T+ +MV+F
Sbjct: 322 GTDVCGSPTLPGGTFNFWGLIENGFLNKDGSPTQGIHYRYDTCSQTPYVYNRTTEIMVAF 381
Query: 187 DDAPAFAAKGEYVKSSGLRGFSMWEAGGDSNDILLDSIRAAAG 315
D+A +FAAKG ++KS+GLRGF+ WEAGGD NDILLDSIR A G
Sbjct: 382 DNAQSFAAKGNFIKSNGLRGFATWEAGGDYNDILLDSIRKAGG 424