BLASTX nr result
ID: Paeonia25_contig00016937
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Paeonia25_contig00016937
(561 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002306381.2| hypothetical protein POPTR_0005s09510g [Popu... 119 7e-25
ref|XP_007043294.1| Uncharacterized protein isoform 2 [Theobroma... 118 9e-25
ref|XP_007043293.1| Uncharacterized protein isoform 1 [Theobroma... 118 9e-25
ref|XP_006484535.1| PREDICTED: uncharacterized protein LOC102607... 117 2e-24
ref|XP_006437584.1| hypothetical protein CICLE_v10031878mg [Citr... 117 2e-24
ref|XP_007224122.1| hypothetical protein PRUPE_ppa015802mg [Prun... 109 5e-22
ref|XP_006380511.1| hypothetical protein POPTR_0007s07720g [Popu... 108 9e-22
ref|XP_006388479.1| hypothetical protein POPTR_0175s00200g [Popu... 108 9e-22
ref|XP_004297739.1| PREDICTED: uncharacterized protein LOC101290... 105 6e-21
ref|XP_006288486.1| hypothetical protein CARUB_v10001745mg [Caps... 103 2e-20
ref|XP_006359305.1| PREDICTED: uncharacterized protein At5g23160... 102 7e-20
ref|NP_196441.1| uncharacterized protein [Arabidopsis thaliana] ... 100 2e-19
ref|XP_006399310.1| hypothetical protein EUTSA_v10014452mg [Eutr... 100 3e-19
ref|XP_002873348.1| predicted protein [Arabidopsis lyrata subsp.... 100 3e-19
ref|XP_004498125.1| PREDICTED: uncharacterized protein At5g23160... 98 1e-18
ref|XP_002523770.1| conserved hypothetical protein [Ricinus comm... 97 3e-18
ref|XP_003534675.1| PREDICTED: uncharacterized protein At5g23160... 96 6e-18
ref|XP_006289314.1| hypothetical protein CARUB_v10002797mg [Caps... 94 2e-17
ref|XP_006394584.1| hypothetical protein EUTSA_v10005318mg, part... 92 9e-17
ref|XP_002874112.1| hypothetical protein ARALYDRAFT_489172 [Arab... 89 1e-15
>ref|XP_002306381.2| hypothetical protein POPTR_0005s09510g [Populus trichocarpa]
gi|550338477|gb|EEE93377.2| hypothetical protein
POPTR_0005s09510g [Populus trichocarpa]
Length = 314
Score = 119 bits (297), Expect = 7e-25
Identities = 59/92 (64%), Positives = 70/92 (76%), Gaps = 1/92 (1%)
Frame = -3
Query: 526 KPHPENDLAV-KKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLLRRSDDIAT 350
KPH END KK DP+VGMSI+++TL+IMLLWG+LCAI+C SAWFYF+P L DD+
Sbjct: 223 KPHKENDRPNGKKLDPLVGMSIVVMTLMIMLLWGKLCAILCASAWFYFVPRLISEDDVKN 282
Query: 349 NTSSNLVNMDMNSEDYKKKVVLEGFLERKRRS 254
+ N+D SEDYKKKVVLEGFLERKR S
Sbjct: 283 GSIQREFNLD--SEDYKKKVVLEGFLERKRHS 312
>ref|XP_007043294.1| Uncharacterized protein isoform 2 [Theobroma cacao]
gi|508707229|gb|EOX99125.1| Uncharacterized protein
isoform 2 [Theobroma cacao]
Length = 286
Score = 118 bits (296), Expect = 9e-25
Identities = 57/104 (54%), Positives = 77/104 (74%), Gaps = 3/104 (2%)
Frame = -3
Query: 559 KRTANTATKLKKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLP 380
KR N L++ +N+ V+K DP++GMSI++VTLIIMLLWGRLCAI+CTSAWFYF P
Sbjct: 181 KRVGNPRMHLREVQRKNNEVVEKIDPVLGMSIIMVTLIIMLLWGRLCAILCTSAWFYFCP 240
Query: 379 LLR---RSDDIATNTSSNLVNMDMNSEDYKKKVVLEGFLERKRR 257
R ++D A ++N ++D++SE+YKKKVVLEGFL+R R
Sbjct: 241 RFRSTINNNDTAVRGTANSSDLDLDSEEYKKKVVLEGFLQRNHR 284
>ref|XP_007043293.1| Uncharacterized protein isoform 1 [Theobroma cacao]
gi|508707228|gb|EOX99124.1| Uncharacterized protein
isoform 1 [Theobroma cacao]
Length = 289
Score = 118 bits (296), Expect = 9e-25
Identities = 57/104 (54%), Positives = 77/104 (74%), Gaps = 3/104 (2%)
Frame = -3
Query: 559 KRTANTATKLKKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLP 380
KR N L++ +N+ V+K DP++GMSI++VTLIIMLLWGRLCAI+CTSAWFYF P
Sbjct: 184 KRVGNPRMHLREVQRKNNEVVEKIDPVLGMSIIMVTLIIMLLWGRLCAILCTSAWFYFCP 243
Query: 379 LLR---RSDDIATNTSSNLVNMDMNSEDYKKKVVLEGFLERKRR 257
R ++D A ++N ++D++SE+YKKKVVLEGFL+R R
Sbjct: 244 RFRSTINNNDTAVRGTANSSDLDLDSEEYKKKVVLEGFLQRNHR 287
>ref|XP_006484535.1| PREDICTED: uncharacterized protein LOC102607466 [Citrus sinensis]
Length = 345
Score = 117 bits (294), Expect = 2e-24
Identities = 57/94 (60%), Positives = 74/94 (78%), Gaps = 2/94 (2%)
Frame = -3
Query: 529 KKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLLR--RSDDI 356
K +N + V KFDP+VGMSI++VTLIIM +WGRLCAI+CTSAWFYF+P LR ++DD
Sbjct: 250 KTGQDDNAMVVNKFDPVVGMSIIVVTLIIMFIWGRLCAILCTSAWFYFVPRLRAIKNDDA 309
Query: 355 ATNTSSNLVNMDMNSEDYKKKVVLEGFLERKRRS 254
AT + ++DMNS++YKK+VVLEGFLER R+
Sbjct: 310 ATMKING--DLDMNSQEYKKRVVLEGFLERDHRT 341
>ref|XP_006437584.1| hypothetical protein CICLE_v10031878mg [Citrus clementina]
gi|557539780|gb|ESR50824.1| hypothetical protein
CICLE_v10031878mg [Citrus clementina]
Length = 369
Score = 117 bits (294), Expect = 2e-24
Identities = 57/94 (60%), Positives = 74/94 (78%), Gaps = 2/94 (2%)
Frame = -3
Query: 529 KKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLLR--RSDDI 356
K +N + V KFDP+VGMSI++VTLIIM +WGRLCAI+CTSAWFYF+P LR ++DD
Sbjct: 274 KTGQDDNAMVVNKFDPVVGMSIIVVTLIIMFIWGRLCAILCTSAWFYFVPRLRAIKNDDA 333
Query: 355 ATNTSSNLVNMDMNSEDYKKKVVLEGFLERKRRS 254
AT + ++DMNS++YKK+VVLEGFLER R+
Sbjct: 334 ATMKING--DLDMNSQEYKKRVVLEGFLERDHRT 365
>ref|XP_007224122.1| hypothetical protein PRUPE_ppa015802mg [Prunus persica]
gi|462421058|gb|EMJ25321.1| hypothetical protein
PRUPE_ppa015802mg [Prunus persica]
Length = 417
Score = 109 bits (272), Expect = 5e-22
Identities = 54/86 (62%), Positives = 65/86 (75%), Gaps = 4/86 (4%)
Frame = -3
Query: 511 NDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLLRRSD----DIATNT 344
N+ K DP+VGMSI++VTLIIML WGRLCAI+CT AWFYFLP S AT T
Sbjct: 323 NNKKKKNSDPLVGMSIIVVTLIIMLFWGRLCAILCTCAWFYFLPRFTSSSAAATATATGT 382
Query: 343 SSNLVNMDMNSEDYKKKVVLEGFLER 266
+S + ++D+NSE+YKKKVVLEGFLER
Sbjct: 383 ASIISDLDLNSEEYKKKVVLEGFLER 408
>ref|XP_006380511.1| hypothetical protein POPTR_0007s07720g [Populus trichocarpa]
gi|550334360|gb|ERP58308.1| hypothetical protein
POPTR_0007s07720g [Populus trichocarpa]
Length = 299
Score = 108 bits (270), Expect = 9e-22
Identities = 59/109 (54%), Positives = 79/109 (72%), Gaps = 8/109 (7%)
Frame = -3
Query: 556 RTANTATK----LKKPHPENDLAV-KKFD-PMVGMSILIVTLIIMLLWGRLCAIICTSAW 395
+T NTA + +K+P END KK D P VGMSI+I+TL++ML WG+LCAI+CTSAW
Sbjct: 193 KTPNTAARSRFTIKRPRKENDHQNGKKSDHPFVGMSIIIMTLVLMLAWGKLCAILCTSAW 252
Query: 394 FYFLPLLRRSDDIATNTSSNLVNMD--MNSEDYKKKVVLEGFLERKRRS 254
FYF+P LR D + S+ L++ + +SE+YKKKVV++GFLER RRS
Sbjct: 253 FYFVPRLRSGDSV----SNGLISAESFYDSEEYKKKVVMDGFLERSRRS 297
>ref|XP_006388479.1| hypothetical protein POPTR_0175s00200g [Populus trichocarpa]
gi|550310250|gb|ERP47393.1| hypothetical protein
POPTR_0175s00200g [Populus trichocarpa]
Length = 299
Score = 108 bits (270), Expect = 9e-22
Identities = 59/109 (54%), Positives = 79/109 (72%), Gaps = 8/109 (7%)
Frame = -3
Query: 556 RTANTATK----LKKPHPENDLAV-KKFD-PMVGMSILIVTLIIMLLWGRLCAIICTSAW 395
+T NTA + +K+P END KK D P VGMSI+I+TL++ML WG+LCAI+CTSAW
Sbjct: 193 KTPNTAARSRFTIKRPRKENDHQNGKKSDHPFVGMSIIIMTLVLMLAWGKLCAILCTSAW 252
Query: 394 FYFLPLLRRSDDIATNTSSNLVNMD--MNSEDYKKKVVLEGFLERKRRS 254
FYF+P LR D + S+ L++ + +SE+YKKKVV++GFLER RRS
Sbjct: 253 FYFVPRLRSEDSV----SNGLISAESFYDSEEYKKKVVMDGFLERSRRS 297
>ref|XP_004297739.1| PREDICTED: uncharacterized protein LOC101290756 [Fragaria vesca
subsp. vesca]
Length = 338
Score = 105 bits (263), Expect = 6e-21
Identities = 52/111 (46%), Positives = 74/111 (66%), Gaps = 13/111 (11%)
Frame = -3
Query: 541 ATKLKKP---HPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLLR 371
A+ +KP E + KK DP+VGM+I++VTL+IM+LWGRLCAI+CT+AWFY LP L
Sbjct: 226 ASSARKPWLVSNEKTMTTKKLDPLVGMTIIMVTLVIMVLWGRLCAILCTAAWFYLLPRLT 285
Query: 370 RSDDIATNTSSNLV----------NMDMNSEDYKKKVVLEGFLERKRRSKY 248
++ + T T+S+ ++D +S++YKKKVVLEGFLER +
Sbjct: 286 QNGNYLTTTASSTAGNTFPHPRDDHLDFSSDEYKKKVVLEGFLERNNHRSH 336
>ref|XP_006288486.1| hypothetical protein CARUB_v10001745mg [Capsella rubella]
gi|482557192|gb|EOA21384.1| hypothetical protein
CARUB_v10001745mg [Capsella rubella]
Length = 262
Score = 103 bits (258), Expect = 2e-20
Identities = 53/106 (50%), Positives = 70/106 (66%), Gaps = 5/106 (4%)
Frame = -3
Query: 559 KRTANTATKLKKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLP 380
KR + + LK N V KFDP++G+SI+I+TL+IML+WGRLCAI+CTSAW Y LP
Sbjct: 153 KRVSTSKPDLKPTRANNGSRVDKFDPVIGISIIILTLMIMLVWGRLCAILCTSAWCYVLP 212
Query: 379 LLRRSDDIAT-----NTSSNLVNMDMNSEDYKKKVVLEGFLERKRR 257
+R + +A S V+ D+NSE YK+KVVL+GFL R R
Sbjct: 213 RVRDAAALAKRKRNGTEGSAYVSSDLNSESYKRKVVLDGFLGRPNR 258
>ref|XP_006359305.1| PREDICTED: uncharacterized protein At5g23160-like [Solanum
tuberosum]
Length = 302
Score = 102 bits (254), Expect = 7e-20
Identities = 52/103 (50%), Positives = 68/103 (66%), Gaps = 2/103 (1%)
Frame = -3
Query: 556 RTANTATKLKKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPL 377
+ N K + E L+ FD ++GMSIL+VTL+IML WG+ CAI+CT AWFYFLP
Sbjct: 195 KKVNDDEKSSQRRRERVLSHDNFDSIMGMSILMVTLLIMLFWGKACAIVCTCAWFYFLPR 254
Query: 376 LRRSDD--IATNTSSNLVNMDMNSEDYKKKVVLEGFLERKRRS 254
+R ++ IA ++D+NS +YKKKVVLEGFLER RS
Sbjct: 255 IRTKNEAVIAGKIDGVAGDVDLNSAEYKKKVVLEGFLERNHRS 297
>ref|NP_196441.1| uncharacterized protein [Arabidopsis thaliana]
gi|8346561|emb|CAB93725.1| putative protein [Arabidopsis
thaliana] gi|26449453|dbj|BAC41853.1| unknown protein
[Arabidopsis thaliana] gi|111074174|gb|ABH04460.1|
At5g08240 [Arabidopsis thaliana]
gi|332003890|gb|AED91273.1| uncharacterized protein
AT5G08240 [Arabidopsis thaliana]
Length = 258
Score = 100 bits (250), Expect = 2e-19
Identities = 49/93 (52%), Positives = 67/93 (72%), Gaps = 1/93 (1%)
Frame = -3
Query: 532 LKKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLLRRSDDIA 353
LK N VK FDP++G+SI+I+TL+IML+WGRLCAI+CTSAW Y LP +R + +A
Sbjct: 162 LKPTRSRNGSRVKPFDPVIGISIIILTLMIMLVWGRLCAILCTSAWCYVLPRVRDAAALA 221
Query: 352 TNTSSNLVNM-DMNSEDYKKKVVLEGFLERKRR 257
+ ++ D+NSE YK+KVVL+GFL R+ R
Sbjct: 222 KRKRNGSASVPDLNSESYKRKVVLDGFLGRQNR 254
>ref|XP_006399310.1| hypothetical protein EUTSA_v10014452mg [Eutrema salsugineum]
gi|557100400|gb|ESQ40763.1| hypothetical protein
EUTSA_v10014452mg [Eutrema salsugineum]
Length = 257
Score = 100 bits (248), Expect = 3e-19
Identities = 49/106 (46%), Positives = 73/106 (68%), Gaps = 4/106 (3%)
Frame = -3
Query: 553 TANTATKLKKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLL 374
T + ++K + V+KFDP++G+SI+I+TL+IML+WGRLCAI+CTSAW Y LP L
Sbjct: 151 TRHGKPEMKPSRAKTRSRVEKFDPVIGISIIILTLMIMLIWGRLCAILCTSAWCYLLPRL 210
Query: 373 RRSDDIA----TNTSSNLVNMDMNSEDYKKKVVLEGFLERKRRSKY 248
+ + +A +++ + D+NSE YK+KVVLEGFL R+ R +
Sbjct: 211 KDAAALAKRKRSSSDGSAYVPDLNSESYKRKVVLEGFLGRQNRVSF 256
>ref|XP_002873348.1| predicted protein [Arabidopsis lyrata subsp. lyrata]
gi|297319185|gb|EFH49607.1| predicted protein
[Arabidopsis lyrata subsp. lyrata]
Length = 252
Score = 100 bits (248), Expect = 3e-19
Identities = 48/92 (52%), Positives = 65/92 (70%)
Frame = -3
Query: 532 LKKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLLRRSDDIA 353
LK N VK FDP++G+SI+I+TL+IML+WGRLCAI+CTSAW Y LP +R + +A
Sbjct: 157 LKPTRSRNGSRVKPFDPVIGISIIILTLMIMLVWGRLCAILCTSAWCYVLPRVRDAAALA 216
Query: 352 TNTSSNLVNMDMNSEDYKKKVVLEGFLERKRR 257
+ D+NS+ YK+KVVL+GFL R+ R
Sbjct: 217 KRKRNGSDVGDLNSQSYKRKVVLDGFLGRQNR 248
>ref|XP_004498125.1| PREDICTED: uncharacterized protein At5g23160-like [Cicer arietinum]
Length = 254
Score = 98.2 bits (243), Expect = 1e-18
Identities = 50/105 (47%), Positives = 68/105 (64%), Gaps = 5/105 (4%)
Frame = -3
Query: 556 RTANTATKLKKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPL 377
+T T+ LK A +D +VGMS+L+VTL+IM+ WGR CAI+CTSAW YF+P
Sbjct: 155 KTKTTSKNLKN-------ARSSYDSIVGMSVLVVTLVIMIFWGRFCAILCTSAWLYFIPR 207
Query: 376 LRRS-----DDIATNTSSNLVNMDMNSEDYKKKVVLEGFLERKRR 257
LR + DDI SN + +D++SE+YKKKV++EG L R R
Sbjct: 208 LRNNVVVNDDDIGPKVKSNDI-VDLDSEEYKKKVIMEGLLGRNNR 251
>ref|XP_002523770.1| conserved hypothetical protein [Ricinus communis]
gi|223536982|gb|EEF38619.1| conserved hypothetical
protein [Ricinus communis]
Length = 282
Score = 97.1 bits (240), Expect = 3e-18
Identities = 49/91 (53%), Positives = 63/91 (69%)
Frame = -3
Query: 529 KKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLLRRSDDIAT 350
KK +N++ K FDP++GMSI++VTLIIMLLWGRLCAI+CT AW YF L ++D
Sbjct: 190 KKDRRKNEMIGKGFDPVIGMSIVMVTLIIMLLWGRLCAILCTCAWLYFTRRL-KTDMSPR 248
Query: 349 NTSSNLVNMDMNSEDYKKKVVLEGFLERKRR 257
D +S++YKK+VVLEGFLER R
Sbjct: 249 QIEKIRGEPDFSSDEYKKRVVLEGFLERDHR 279
>ref|XP_003534675.1| PREDICTED: uncharacterized protein At5g23160-like [Glycine max]
Length = 253
Score = 95.9 bits (237), Expect = 6e-18
Identities = 49/109 (44%), Positives = 67/109 (61%), Gaps = 7/109 (6%)
Frame = -3
Query: 559 KRTANTATKLKKPHPENDLAVKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLP 380
KR A + + A +DP+VGMS+L+VTL+IML WGRLCAI+CTSAW Y P
Sbjct: 146 KRQARRLSSSMQAQTTPKKAQNAYDPVVGMSVLVVTLVIMLFWGRLCAILCTSAWLYCTP 205
Query: 379 LLRRS-------DDIATNTSSNLVNMDMNSEDYKKKVVLEGFLERKRRS 254
R+S +D+ T S +D++SE+YKKKV++EG L R R+
Sbjct: 206 RFRKSGGYVNDDEDLQTTKSK---EVDLDSEEYKKKVIMEGLLGRNHRT 251
>ref|XP_006289314.1| hypothetical protein CARUB_v10002797mg [Capsella rubella]
gi|482558020|gb|EOA22212.1| hypothetical protein
CARUB_v10002797mg [Capsella rubella]
Length = 252
Score = 94.4 bits (233), Expect = 2e-17
Identities = 47/86 (54%), Positives = 61/86 (70%), Gaps = 5/86 (5%)
Frame = -3
Query: 499 VKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLLRRSDDIATNTSSNLV--- 329
V KFDP++G+SI+ +TL+IML WGRLCAI+CTS W YFLP L+ + A S
Sbjct: 166 VGKFDPVIGISIITLTLMIMLTWGRLCAILCTSTWCYFLPRLKEATTAAKCKRSGSCKAV 225
Query: 328 --NMDMNSEDYKKKVVLEGFLERKRR 257
++D+NS+ YKKKVVLEGFL R+ R
Sbjct: 226 PGDLDLNSDAYKKKVVLEGFLVRQYR 251
>ref|XP_006394584.1| hypothetical protein EUTSA_v10005318mg, partial [Eutrema
salsugineum] gi|557091223|gb|ESQ31870.1| hypothetical
protein EUTSA_v10005318mg, partial [Eutrema salsugineum]
Length = 154
Score = 92.0 bits (227), Expect = 9e-17
Identities = 47/90 (52%), Positives = 62/90 (68%), Gaps = 9/90 (10%)
Frame = -3
Query: 499 VKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLL---------RRSDDIATN 347
V + DP++G+ I+++TL+IML+WGRLCAI+CTS W YFLP L +RS N
Sbjct: 62 VSRVDPVIGIIIIMLTLMIMLMWGRLCAILCTSTWCYFLPRLKEAAIAAKRKRSGGGKAN 121
Query: 346 TSSNLVNMDMNSEDYKKKVVLEGFLERKRR 257
S ++D+NSE YKKKVVLEGFL R+ R
Sbjct: 122 GVSFPGDLDLNSEAYKKKVVLEGFLVRQHR 151
>ref|XP_002874112.1| hypothetical protein ARALYDRAFT_489172 [Arabidopsis lyrata subsp.
lyrata] gi|297319949|gb|EFH50371.1| hypothetical protein
ARALYDRAFT_489172 [Arabidopsis lyrata subsp. lyrata]
Length = 273
Score = 88.6 bits (218), Expect = 1e-15
Identities = 46/93 (49%), Positives = 60/93 (64%), Gaps = 12/93 (12%)
Frame = -3
Query: 499 VKKFDPMVGMSILIVTLIIMLLWGRLCAIICTSAWFYFLPLL------------RRSDDI 356
V K DP++G+SI+++TL+IML WGRLCAI+CTS W Y LP L +R+
Sbjct: 178 VAKLDPVIGISIIMLTLMIMLTWGRLCAILCTSTWCYILPRLKEAATAVAVAKRKRNGSG 237
Query: 355 ATNTSSNLVNMDMNSEDYKKKVVLEGFLERKRR 257
S ++D+NSE YKKKVVLEGFL R+ R
Sbjct: 238 KGEEGSFQGDLDLNSEAYKKKVVLEGFLVRQHR 270