BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Paeonia24_contig00013134
         (3157 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...  1230   0.0  
ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|50...  1192   0.0  
ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prun...  1181   0.0  
ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1154   0.0  
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...  1136   0.0  
ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citr...  1134   0.0  
gb|EXB39337.1| Isoamylase 2 [Morus notabilis]                        1128   0.0  
ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1105   0.0  
ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Popu...  1092   0.0  
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...  1048   0.0  
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1048   0.0  
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1032   0.0  
ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...  1022   0.0  
ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Sola...  1019   0.0  
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|...  1018   0.0  
ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1015   0.0  
ref|XP_007162398.1| hypothetical protein PHAVU_001G148700g [Phas...  1009   0.0  
ref|XP_004493650.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1001   0.0  
ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] gi|42571321...   978   0.0  
gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]             977   0.0  

>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score = 1230 bits (3182), Expect = 0.0
 Identities = 621/886 (70%), Positives = 712/886 (80%), Gaps = 13/886 (1%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKT---EVAQKFT 563
            M TL+PS+ IRP CVNCGA +S KL+    YI GNK   GLEK+ + RK     VAQ  T
Sbjct: 1    MTTLIPSLVIRPCCVNCGARDSSKLVAATHYICGNKVAHGLEKMDLERKEILGVVAQNAT 60

Query: 564  KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 743
            +N  RN+N+K+ ATS I++ +TE R +T TE  EL+  L+YLF TEIGG VKV VGKKN 
Sbjct: 61   RNCFRNINWKVSATSHIAIEETENRYST-TESEELESRLNYLFLTEIGGQVKVIVGKKNK 119

Query: 744  KYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSS 923
            KY V IEVSSL   NSD++LILSWG+FRS+SSC MPVDFQN      + T E PF ++SS
Sbjct: 120  KYIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERSS 179

Query: 924  GIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGLS 1103
            G F   L FEA   P YLSFLLKS+LD D +SS+IRSHRKTNFC+PVGFG G PAPLGLS
Sbjct: 180  GSFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLS 239

Query: 1104 FRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNFV 1283
            F  DGS NFA FSRNA GV+LCLYD   +DKPALEIDLDPYVNR+GDIWHAS+E + +FV
Sbjct: 240  FSSDGSPNFAFFSRNAGGVVLCLYDGT-SDKPALEIDLDPYVNRTGDIWHASMESVGSFV 298

Query: 1284 SYGYR-----FRGEGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAFDW 1448
            SYGYR      +  G+   TE V LDPYAK+I N+ SD HG   P   LG++ +EPAF+W
Sbjct: 299  SYGYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLK-PQPRLGELQKEPAFNW 357

Query: 1449 SGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLLE 1628
            + DV P +PMEKLVVYRLNVMHFTKD+SSQ+  ++AGTFSGL EKLHHFKDLGVNAVLLE
Sbjct: 358  NDDVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLE 417

Query: 1629 PIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTHT 1808
            PIF+FDEQ GPY P HFFSPMN+YGP++G VS INS+KEMVK +HA+GIEVLLEVVFTHT
Sbjct: 418  PIFSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTHT 477

Query: 1809 VEGGALQGIDDSSYY-MNEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFIN 1985
             E GALQGIDDS YY +N  A+LG RNALNCNY IVQQMIVDSLR+WV EF VDGFCFIN
Sbjct: 478  AESGALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVDGFCFIN 537

Query: 1986 SSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEMN 2165
            +SSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIAD WDP +M PK+IRFPHW RWAE+N
Sbjct: 538  ASSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWKRWAEVN 597

Query: 2166 SRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFSN 2345
            +RFCNDVR FL+G+ L SD ATRLCGSGDIF D RGPAFSFNF  KN GLPLVDLVSFS+
Sbjct: 598  TRFCNDVRNFLRGEGL-SDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVDLVSFSS 656

Query: 2346 TKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGSL 2525
            ++LASELSWNCG+EGPTNK  VLERRLKQIRN+LF+LY+SLGVPILNMGDECGQS GGS 
Sbjct: 657  SELASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQSSGGSP 716

Query: 2526 AYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSEL 2705
            AY DRKPF+WN++KTGFGIQT QFISFL+SLR RR DLLQ+R+FLK E+IDWHGSDQS  
Sbjct: 717  AYGDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHGSDQSPP 776

Query: 2706 KWDDPSSKFLAMTLKADIAE----SERNPMWGDLFIAINAADCSECVILPPPPEGMAWLR 2873
            +WDDPSSKFLAMTLKA+  E    SE + + GDLFIA N AD S  VILPPPP GM W R
Sbjct: 777  RWDDPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGMVWHR 836

Query: 2874 LVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFETCSPSG 3011
            LVDTALPFPGFF+ +GE +L++ +GL  Y+M S+SC LFE  +  G
Sbjct: 837  LVDTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEANTLDG 882


>ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|508702938|gb|EOX94834.1|
            Debranching enzyme 1 [Theobroma cacao]
          Length = 867

 Score = 1192 bits (3085), Expect = 0.0
 Identities = 592/875 (67%), Positives = 701/875 (80%), Gaps = 8/875 (0%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKT---EVAQKFT 563
            MATL PS+AI PHC+N GATE  KL F  +Y+  +K    L++I +GRK    EVAQ   
Sbjct: 1    MATLPPSLAIGPHCLNSGATELSKLSFVTRYLCKSKMGQSLQRIDVGRKLFSGEVAQNVM 60

Query: 564  KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 743
            ++   NL+ +  A S++SV QTEQ + TST++ EL++  +YLFRTEIGG VKVFV K+++
Sbjct: 61   QSRYWNLDLRCFAASRVSVEQTEQ-IFTSTQVDELKKLSTYLFRTEIGGQVKVFVRKRSV 119

Query: 744  KYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSS 923
             + V IEVSSL     +S+L+LS GI RSD       D +N         IETPF  KSS
Sbjct: 120  NHVVDIEVSSLQLSGDESQLVLSGGIHRSDH------DIKNRI-------IETPFIAKSS 166

Query: 924  GIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGLS 1103
                 +L FEAK+ P Y SFLLK+    + + SEIR+HRKTNFCVPVGF  G PAPLGLS
Sbjct: 167  SELALELEFEAKEAPFYFSFLLKAPSGANLSGSEIRTHRKTNFCVPVGFNQGYPAPLGLS 226

Query: 1104 FRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNFV 1283
            F  DGS NFA++SRNAE ++LCLYDD  ++KPALE+DLDP+VNR+GDIWHASIE    FV
Sbjct: 227  FSTDGSMNFAVYSRNAESLVLCLYDDTASEKPALELDLDPFVNRTGDIWHASIEGSWTFV 286

Query: 1284 SYGYRFRGEGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAFDWSGDVR 1463
             YGYR +G+ D F+ ERV+LDPYA+IIG+++ +HH SG  +KHLG++ +EPAFDWS DV 
Sbjct: 287  RYGYRCKGDTDAFNAERVLLDPYARIIGSSIPNHHESGLLLKHLGRLCKEPAFDWSSDVC 346

Query: 1464 PNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLLEPIFAF 1643
            PNLPMEKLVVYRLNV+HFT+DKSS+LP +V GTF+G+TEK+ H KDLGVNAVLLEPIF F
Sbjct: 347  PNLPMEKLVVYRLNVLHFTEDKSSKLPADVVGTFAGVTEKVQHLKDLGVNAVLLEPIFTF 406

Query: 1644 DEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTHTVEGGA 1823
            DEQ GPY PCHFFSP NLYGP+NG VSAINS+KEMVK +HA+G+EVLLEVVFTHT EGGA
Sbjct: 407  DEQKGPYFPCHFFSPTNLYGPSNGSVSAINSIKEMVKNLHANGMEVLLEVVFTHTAEGGA 466

Query: 1824 LQGIDDSSYYM-NEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFINSSSLL 2000
            LQG+DD SYY  N V +L  RNALNCNYP+VQ+MI+DSLRHWV EF +DGFCFIN+S LL
Sbjct: 467  LQGLDDLSYYYRNRVQDLERRNALNCNYPVVQRMILDSLRHWVTEFHIDGFCFINASCLL 526

Query: 2001 RGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEMNSRFCN 2180
            RGF+GE+LSRPPL+E IAFDPLLSKTKIIAD W PHDM PK+IRFPHW +WAEMN++FC 
Sbjct: 527  RGFYGEHLSRPPLIEAIAFDPLLSKTKIIADCWYPHDMMPKEIRFPHWKKWAEMNTKFCT 586

Query: 2181 DVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFSNTKLAS 2360
            D+R FL+G+  LS LATRLCGSGDIFSD RGPAFSFN+IAKN GLPLVDLVSFS  ++AS
Sbjct: 587  DIRNFLRGEGALSSLATRLCGSGDIFSDGRGPAFSFNYIAKNFGLPLVDLVSFSKAEIAS 646

Query: 2361 ELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGSLAYSDR 2540
            ELSWNCG EGPTNK AVLERRLKQIRN+LF+L+ISLGVP+LNMGDECGQS GGSL+Y  R
Sbjct: 647  ELSWNCGVEGPTNKTAVLERRLKQIRNFLFILFISLGVPVLNMGDECGQSSGGSLSYGSR 706

Query: 2541 KPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSELKWDDP 2720
            K  DWNA+ TGFGIQTTQFISFL+SLR+RR DLLQKR+FLK ENI+W+GSDQS   W+DP
Sbjct: 707  KLLDWNAMTTGFGIQTTQFISFLSSLRMRRSDLLQKRSFLKEENIEWYGSDQSPPGWEDP 766

Query: 2721 SSKFLAMTLKADIAE----SERNPMWGDLFIAINAADCSECVILPPPPEGMAWLRLVDTA 2888
            S KFLAMTLKAD  E    SE + + GDL IAINAAD +E +ILPPPPEG+AW RLVDTA
Sbjct: 767  SCKFLAMTLKADKEENQLSSEASRLKGDLLIAINAADKAEIIILPPPPEGLAWRRLVDTA 826

Query: 2889 LPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
            LP+PGFFS +G+ VLEQ  GL+AYEM S SCTLFE
Sbjct: 827  LPYPGFFSADGKAVLEQMMGLFAYEMKSLSCTLFE 861


>ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prunus persica]
            gi|462397150|gb|EMJ02949.1| hypothetical protein
            PRUPE_ppa001199mg [Prunus persica]
          Length = 883

 Score = 1181 bits (3054), Expect = 0.0
 Identities = 592/884 (66%), Positives = 691/884 (78%), Gaps = 14/884 (1%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKT---EVAQKFT 563
            MATL  S+AI+  C NCG TES KL     +   +KA+ G  K+   RK    EV Q   
Sbjct: 1    MATLPLSIAIQACCFNCGITESSKLTATNGFRHRDKAMHGFVKLDAERKLVFREVVQNIN 60

Query: 564  KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 743
            +N  R+ N K+ A SQ+SV   EQR +T TE  E+ +  +YLFRTEIG  V VFV K+N 
Sbjct: 61   ENLPRDHNLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFVRKRNA 120

Query: 744  KYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSS 923
            KY V IEV S    ++D  L+L WG++R+DSSC +P+DF++S     T T+ETP  Q SS
Sbjct: 121  KYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPLIQTSS 180

Query: 924  GIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGLS 1103
            G FT +L FEAKQ P Y SF+L S  D + +  EIRSHRKTNFCVPVGFG G P PLGL+
Sbjct: 181  GRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLT 240

Query: 1104 FRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNFV 1283
            F  DGS NFAIFSRNAE V LCLYD+  A+KPALE+DLDPYVNRSGDIWH S     +FV
Sbjct: 241  FSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWSFV 300

Query: 1284 SYGYRFRGE-----GDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAFDW 1448
            SYGY+F+G       + FD   V+LDPYAK+I  ++ ++HG+G  +K+LG++  EPAFDW
Sbjct: 301  SYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTG--LKYLGRLCEEPAFDW 358

Query: 1449 SGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLLE 1628
            +GDVRP+L MEKLVVYRLNV  FT+ KSSQLP N+ G+FSGLTEKL HFKDLGVNAVLLE
Sbjct: 359  AGDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVLLE 418

Query: 1629 PIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTHT 1808
            PIF FDEQ GPY P HFFSPM+ +GP+ G VSA+NSMKEMV+  HA+GIEVLLEVVFTHT
Sbjct: 419  PIFPFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLLEVVFTHT 478

Query: 1809 VEGGALQGIDDSSYY-MNEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFIN 1985
             EG ALQGID SSYY +NEV +L  RNALNCNYPIVQQ+++DSLR+WV EF VDGF FIN
Sbjct: 479  AEGEALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHVDGFFFIN 538

Query: 1986 SSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEMN 2165
            +SSLLRGF+GEYLSRPPLVE IAFDPLLSKTKIIAD WDPH MAPK+  FPHW RWAE+N
Sbjct: 539  ASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHWRRWAEVN 598

Query: 2166 SRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFSN 2345
            ++FCNDVR FL+G+ LLSDLATRLCG+GDIFSD RGPAF+FNFI++NSGLPLVDLVSFS 
Sbjct: 599  TKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLVSFSG 658

Query: 2346 TKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGSL 2525
             +LASELSWNCGEEGPTNK AVLERRLKQIRN+LF+L++SLGVP+LNMGDECGQS GGS 
Sbjct: 659  VELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSTGGSP 718

Query: 2526 AYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSEL 2705
            AYSDRK FDWNAL+TGF  QTTQFI+FL+S R RR DLLQKRNFLK ENI W+ SDQ+  
Sbjct: 719  AYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWYESDQTPP 778

Query: 2706 KWDDPSSKFLAMTLKADIAE-----SERNPMWGDLFIAINAADCSECVILPPPPEGMAWL 2870
            +W+DPS KFLAM LKAD  E      E +  WGDLF+A +AAD SE V+LPPP EGM W 
Sbjct: 779  RWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPPLEGMGWR 838

Query: 2871 RLVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFETCS 3002
            RLVDTALPFPGFFST+GEPV+EQ  GL+AYEM S+SC LFE  S
Sbjct: 839  RLVDTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEARS 882


>ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like isoform X1 [Citrus
            sinensis] gi|568852193|ref|XP_006479764.1| PREDICTED:
            isoamylase 2, chloroplastic-like isoform X2 [Citrus
            sinensis] gi|568852195|ref|XP_006479765.1| PREDICTED:
            isoamylase 2, chloroplastic-like isoform X3 [Citrus
            sinensis]
          Length = 889

 Score = 1154 bits (2986), Expect = 0.0
 Identities = 581/885 (65%), Positives = 683/885 (77%), Gaps = 18/885 (2%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATES--PKLIFPAQ-----YIIGNKAICGLEKIHMGRKTEVA 551
            MATL PS+A RP C NCGA ES  P  +  A      Y+  +K      ++ +  KT  A
Sbjct: 1    MATLAPSLAFRPCCFNCGAVESSPPSKLCAASASASHYVFKSKCTRRPARLEV-EKTLSA 59

Query: 552  QKFTKNPLRNLNFKLLATS-QISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFV 728
            Q          +F+  A+S ++SV +T+   T +TE+ ELQ+  +YLFRT++GG V V V
Sbjct: 60   QVCQNAVTTRPDFRACASSSRVSVDETQPVFTPTTEVDELQKVSTYLFRTDLGGLVSVSV 119

Query: 729  GKKNIKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPF 908
            G+KN+ YAV I+VSSL       +L+L WG++ SDSS  M +  Q     A+TG+IETPF
Sbjct: 120  GEKNVNYAVTIDVSSLQTTRGGHKLVLIWGVYTSDSSSYMHLGSQCFTPDAKTGSIETPF 179

Query: 909  TQKSSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPA 1088
            T  S   F  +L FEAKQTP YLSFLLKS L   S   EIR+H    FCVPVG  +G+P+
Sbjct: 180  TPTSFDTFALELGFEAKQTPFYLSFLLKSPLRDGSGDFEIRNHLSAKFCVPVGLNAGVPS 239

Query: 1089 PLGLSFRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEI 1268
            P+GLSF +DGS NFAIFSRNA+GV+LCLYDD  AD+PALE+DLDPY+NRSGDIWHAS+E 
Sbjct: 240  PMGLSFSIDGSLNFAIFSRNAQGVVLCLYDDTTADRPALELDLDPYINRSGDIWHASMES 299

Query: 1269 LPNFVSYGYRFRG---EGDEFDT--ERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSRE 1433
              NFVSYGYRF+G   +GD + +  E V+LDPYAKII N++ +HH  G P K+LG++ +E
Sbjct: 300  TWNFVSYGYRFKGSFSQGDGYKSHLESVLLDPYAKIIVNSIPNHHDLGLPPKYLGRLCKE 359

Query: 1434 PAFDWSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVN 1613
            P FDW GDV  NLPMEKLVVYRLNVM F++ KSS+LP ++AGTFSG+TEK+HH KDLGVN
Sbjct: 360  PDFDWGGDVHLNLPMEKLVVYRLNVMRFSEHKSSKLPPDIAGTFSGVTEKVHHLKDLGVN 419

Query: 1614 AVLLEPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEV 1793
            A+LLEPI +FDEQ GPY P HFFSP  L+GP+ G +SAINSMKEMVK +HA+GIEVLLEV
Sbjct: 420  AILLEPILSFDEQKGPYFPRHFFSPTKLHGPSRGSISAINSMKEMVKKLHANGIEVLLEV 479

Query: 1794 VFTHTVEGGALQGIDDSSYYM-NEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDG 1970
            VFT T +G ALQGIDDSSYY  +    + T N LNCNYP VQQMI++SLRHWV EF +DG
Sbjct: 480  VFTRTADG-ALQGIDDSSYYYAHRGEGIETTNVLNCNYPTVQQMILNSLRHWVTEFHIDG 538

Query: 1971 FCFINSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMR 2150
            FCFIN+SSLLRGFHGEYLSRPPL+E IAFDPLLSK K+IAD WDPH +APKD RFPHW R
Sbjct: 539  FCFINASSLLRGFHGEYLSRPPLIEAIAFDPLLSKAKLIADYWDPHGVAPKDTRFPHWKR 598

Query: 2151 WAEMNSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDL 2330
            WAE+N+ FCNDVR F +G+ LLSDLATRLCGSGDIFSD RGPAFSFN+IA+N+GLPLVDL
Sbjct: 599  WAELNTNFCNDVRNFFRGEGLLSDLATRLCGSGDIFSDGRGPAFSFNYIARNTGLPLVDL 658

Query: 2331 VSFSNTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQS 2510
            VSFS   LASELSWNCGEEGPT K AVLERRLKQIRN+LFVLY+SLGVPILNMGDECGQS
Sbjct: 659  VSFSGGGLASELSWNCGEEGPTTKTAVLERRLKQIRNFLFVLYVSLGVPILNMGDECGQS 718

Query: 2511 YGGSLAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGS 2690
              GS +Y+DRKPFDWNAL TGFGIQ T+FISFL+S R++R DLL++RNFLK ENIDWHGS
Sbjct: 719  SWGSPSYADRKPFDWNALATGFGIQITEFISFLSSFRLKRSDLLEQRNFLKEENIDWHGS 778

Query: 2691 DQSELKWDDPSSKFLAMTLKADIAE----SERNPMWGDLFIAINAADCSECVILPPPPEG 2858
            D S  +W+DP  KFLAM LK D AE    SE +   GDL+IA NAAD SE V+LPPPPEG
Sbjct: 779  DHSPPRWEDPDCKFLAMRLKVDKAESQLSSESSQTKGDLYIACNAADHSESVVLPPPPEG 838

Query: 2859 MAWLRLVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
            M W  LVDTALPFPGFFST G+PVLEQ AGLY YEM  YSCTLFE
Sbjct: 839  MTWHHLVDTALPFPGFFSTEGKPVLEQMAGLYTYEMKPYSCTLFE 883


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score = 1136 bits (2939), Expect = 0.0
 Identities = 567/876 (64%), Positives = 681/876 (77%), Gaps = 9/876 (1%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQKFTKNP 572
            MAT++PS AIRP    CGATES KL      + GNK   G   +      +    + ++P
Sbjct: 1    MATIIPSFAIRPCYCVCGATESSKLA-----VTGNKDF-GRLVLDQRLFNQRLHFWKQSP 54

Query: 573  LRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNIKYA 752
              +L+ ++ + +++ V QTE R +TS  + EL +  +YLFRT+ GGHVKV V KKN KYA
Sbjct: 55   HWSLDSRVCSAARVPVQQTELRFSTSAPLDELNKVSTYLFRTQFGGHVKVLVRKKNAKYA 114

Query: 753  VYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSSGIF 932
            VYIEVSSL    +D  L+L WGI+RSDSSC MP+D QN A  AR   ++T   Q S G F
Sbjct: 115  VYIEVSSLELGTTDYRLMLIWGIYRSDSSCFMPLDSQNFAPNARK--MDTALVQNSFGTF 172

Query: 933  TTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGLSFRV 1112
              +L FE KQTP YLSFLLKS L+ D++  EI++H+  NFCVP+GF SG P+PLGLSF  
Sbjct: 173  ALELEFEPKQTPFYLSFLLKSKLNTDASGLEIKNHKNANFCVPIGFNSGDPSPLGLSFST 232

Query: 1113 DGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNFVSYG 1292
            DGS NFA FSRN EG++LCLYDD+  DKPALE+DLDPYVNR+GD+WHAS+E    F SYG
Sbjct: 233  DGSMNFAFFSRNVEGLVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASLEGAWTFTSYG 292

Query: 1293 YR-----FRGEGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAFDWSGD 1457
            YR      +G   + D E V+LDPYA++I ++++DH GS    K+LG++  EPAF+W  D
Sbjct: 293  YRCKGAILQGNTSKVDMECVLLDPYARVIASSMTDH-GSRLSAKYLGRLCEEPAFEWGSD 351

Query: 1458 VRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLLEPIF 1637
            +RPNL MEKL+VYRLNV  FT+ KS +L  ++AGTF+GL EK+ HF++LGVNAVLLEPIF
Sbjct: 352  IRPNLAMEKLIVYRLNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVLLEPIF 411

Query: 1638 AFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTHTVEG 1817
             FDEQ GPY P HFFSP N+YGP+ G +SAI SMKEMVK +HA+ IEVLLEVVFTHT EG
Sbjct: 412  PFDEQKGPYFPYHFFSPSNIYGPSGGSISAITSMKEMVKELHANRIEVLLEVVFTHTAEG 471

Query: 1818 GALQGIDDSSYYMNEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFINSSSL 1997
            GALQGIDD SYY  + +++ +RNALNCNYPIVQ+MI+DSL+HWV EF +DGFCFIN+S+L
Sbjct: 472  GALQGIDDFSYYYTK-SSMDSRNALNCNYPIVQRMILDSLQHWVTEFHIDGFCFINASAL 530

Query: 1998 LRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEMNSRFC 2177
            L GFHGE+LSRPPLVE IAFDP+LSKTKIIAD W P    PK+  FPHW RWAE+N +FC
Sbjct: 531  LTGFHGEHLSRPPLVEAIAFDPILSKTKIIADPWHPEHRIPKETCFPHWKRWAEINPKFC 590

Query: 2178 NDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFSNTKLA 2357
             DVR FL+G++LL DLATRLCGSGDIFS+ RGPAFSFN+IA+NSGLPLVDLVSFS  +L 
Sbjct: 591  IDVRNFLRGESLLGDLATRLCGSGDIFSNGRGPAFSFNYIARNSGLPLVDLVSFSGGELG 650

Query: 2358 SELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGSLAYSD 2537
            SELSWNCGEEGPTNK AVLERRLKQIRNYLF+LY+SLGVP+LNMGDECGQS  GS++Y D
Sbjct: 651  SELSWNCGEEGPTNKTAVLERRLKQIRNYLFILYVSLGVPVLNMGDECGQSSRGSISYGD 710

Query: 2538 RKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSELKWDD 2717
            RKPFDWNAL T FG Q TQFISFL+SLR+RR DLLQKRNFLK ENIDWHG+DQS  +W+D
Sbjct: 711  RKPFDWNALSTSFGNQMTQFISFLSSLRMRRSDLLQKRNFLKEENIDWHGNDQSPPRWED 770

Query: 2718 PSSKFLAMTLKADIAE----SERNPMWGDLFIAINAADCSECVILPPPPEGMAWLRLVDT 2885
            P+ KFLAMTLK D AE    SE + + GDLF+A NAA  +E VILPP PEGM W RLVDT
Sbjct: 771  PTCKFLAMTLKIDKAESQLSSEPSNIKGDLFMAFNAAGHAESVILPPVPEGMIWRRLVDT 830

Query: 2886 ALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
            ALPFPGFFS +GEPV+EQ AGL AY+M+S+SCTLFE
Sbjct: 831  ALPFPGFFSEDGEPVVEQIAGLIAYKMNSHSCTLFE 866


>ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citrus clementina]
            gi|557546394|gb|ESR57372.1| hypothetical protein
            CICLE_v10023436mg [Citrus clementina]
          Length = 840

 Score = 1134 bits (2932), Expect = 0.0
 Identities = 571/877 (65%), Positives = 662/877 (75%), Gaps = 10/877 (1%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQKFTKNP 572
            MATL PS+A RP C NCGA ES                                    +P
Sbjct: 1    MATLAPSLAFRPCCFNCGAVES------------------------------------SP 24

Query: 573  LRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNIKYA 752
               L     + S   V       T +TE+ ELQ+  +YLFRT++GG V V VG+KN+ YA
Sbjct: 25   PSKLCAASASASHYPV------FTPTTEVDELQKVSTYLFRTDLGGLVSVSVGEKNVNYA 78

Query: 753  VYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSSGIF 932
            V I+VSSL       +L+L WG++ SDSS  M +  Q     A+TG+IETPFT  S   F
Sbjct: 79   VTIDVSSLQTTRGGHKLVLIWGVYTSDSSSYMHLGSQCFTPDAKTGSIETPFTPTSFDTF 138

Query: 933  TTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGLSFRV 1112
              +L FEAKQTP YLSFLLKS L   S   EIR+H    FCVPVG  +G+P+P+GLSF +
Sbjct: 139  ALELGFEAKQTPFYLSFLLKSPLRDGSGDFEIRNHLSAKFCVPVGLNAGVPSPMGLSFSI 198

Query: 1113 DGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNFVSYG 1292
            DGS NFAIFSRNA+GV+LCLYDD  AD+PALE+DLDPY+NRSGDIWHAS+E   NFVSYG
Sbjct: 199  DGSLNFAIFSRNAQGVVLCLYDDTTADRPALELDLDPYINRSGDIWHASMESTWNFVSYG 258

Query: 1293 YRFRG---EGDEFDT--ERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAFDWSGD 1457
            YRF+G   +GD + +  E V+LDPYAKII N++ +HH  G P K+LG++ +EP FDW GD
Sbjct: 259  YRFKGSFSQGDGYKSHLESVLLDPYAKIIVNSIPNHHDLGLPPKYLGRLCKEPDFDWGGD 318

Query: 1458 VRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLLEPIF 1637
            V  NLPMEKLVVYRLNVM F++ KSS+LP ++AGTFSG+TEK+HH KDLGVNA+LLEPI 
Sbjct: 319  VHLNLPMEKLVVYRLNVMRFSEHKSSKLPPDIAGTFSGVTEKVHHLKDLGVNAILLEPIL 378

Query: 1638 AFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTHTVEG 1817
            +FDEQ GPY P HFFSP  L+GP+ G +SAINSMKEMVK +HA+GIEVLLEVVFT T +G
Sbjct: 379  SFDEQKGPYFPRHFFSPTKLHGPSRGSISAINSMKEMVKKLHANGIEVLLEVVFTRTADG 438

Query: 1818 GALQGIDDSSYYM-NEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFINSSS 1994
             ALQGIDDSSYY  +    + T N LNCNYP VQQMI++SLRHWV EF +DGFCFIN+SS
Sbjct: 439  -ALQGIDDSSYYYAHRGEGIETTNVLNCNYPTVQQMILNSLRHWVTEFHIDGFCFINASS 497

Query: 1995 LLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEMNSRF 2174
            LLRGFHGEYLSRPPL+E IAFDPLLSK K+IAD WDPH +APKD RFPHW RWAE+N+ F
Sbjct: 498  LLRGFHGEYLSRPPLIEAIAFDPLLSKAKLIADYWDPHGVAPKDTRFPHWKRWAELNTNF 557

Query: 2175 CNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFSNTKL 2354
            CNDVR F +G+ LLSDLATRLCGSGDIFSD RGPAFSFN+IA+N+GLPLVDLVSFS   L
Sbjct: 558  CNDVRNFFRGEGLLSDLATRLCGSGDIFSDGRGPAFSFNYIARNTGLPLVDLVSFSGGGL 617

Query: 2355 ASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGSLAYS 2534
            ASELSWNCGEEGPT K AVLERRLKQIRN+LFVLY+SLGVPILNMGDECGQS  GS +Y+
Sbjct: 618  ASELSWNCGEEGPTTKTAVLERRLKQIRNFLFVLYVSLGVPILNMGDECGQSSWGSPSYA 677

Query: 2535 DRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSELKWD 2714
            DRKPFDWNAL TGFGIQ T+FISFL+S R++R DLL++RNFLK ENIDWHGSD S  +W+
Sbjct: 678  DRKPFDWNALATGFGIQITEFISFLSSFRLKRSDLLEQRNFLKEENIDWHGSDHSPPRWE 737

Query: 2715 DPSSKFLAMTLKADIAE----SERNPMWGDLFIAINAADCSECVILPPPPEGMAWLRLVD 2882
            DP  KFLAM LK D AE    SE +   GDL+IA NAAD SE V+LPPPPEGM W  LVD
Sbjct: 738  DPDCKFLAMRLKVDKAESQLSSESSQTKGDLYIACNAADHSESVVLPPPPEGMTWHHLVD 797

Query: 2883 TALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
            TALPFPGFFST G+PVLEQ AGLY YEM  YSCTLFE
Sbjct: 798  TALPFPGFFSTEGKPVLEQMAGLYTYEMKPYSCTLFE 834


>gb|EXB39337.1| Isoamylase 2 [Morus notabilis]
          Length = 825

 Score = 1128 bits (2917), Expect = 0.0
 Identities = 565/822 (68%), Positives = 649/822 (78%), Gaps = 7/822 (0%)
 Frame = +3

Query: 558  FTKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKK 737
            F K P RNL+ ++ ATS++S+   ++RL+ +T++ E ++  +YLFRTE+GG V VFV KK
Sbjct: 12   FEKMP-RNLSVEVNATSRVSIEPVQKRLSRNTKVEEAEKVSTYLFRTEVGGLVNVFVRKK 70

Query: 738  NIKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQK 917
             +   VY+EVSSL    SD  L LSWGI+RSDSS  +P  F+ S        +ETPFT+ 
Sbjct: 71   KVNCTVYVEVSSLELPRSDGTLGLSWGIYRSDSSSFLPSHFETST------PVETPFTKN 124

Query: 918  SSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLG 1097
            S G +T +  FEAK+ P YLSFLLKS  D DS+  +IRSHRKTNFCVP+GF SG P PLG
Sbjct: 125  SFGRYTVEFEFEAKEIPCYLSFLLKSPRDNDSSGLDIRSHRKTNFCVPLGFSSGHPTPLG 184

Query: 1098 LSFRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPN 1277
            LSF  DGS NF+IFSR+AE V+LCLYDD  AD PALE+DLDPYVNR+GDIWHAS E    
Sbjct: 185  LSFSRDGSLNFSIFSRSAESVVLCLYDDTTADNPALELDLDPYVNRTGDIWHASFESSST 244

Query: 1278 FVSYGYRFRG---EGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAFDW 1448
            FVSYGYR +G   +G + +  R++LDPYAKIIG + S  HG G   K+LG++S+EP FDW
Sbjct: 245  FVSYGYRLKGSRLKGKKDEDARIVLDPYAKIIGKSTSSDHGIG--PKYLGRISKEPGFDW 302

Query: 1449 SGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLLE 1628
            SGD+RPNLP+EKL VYRLNVMHFT+ KSSQL  ++AGTFSGL EKL HF DLGVNAVLLE
Sbjct: 303  SGDIRPNLPIEKLTVYRLNVMHFTEHKSSQLSPDIAGTFSGLIEKLEHFTDLGVNAVLLE 362

Query: 1629 PIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTHT 1808
            PIF FDEQ GP  PCHFFSP +LYGP+ G VSAIN+MKEMVK +HASGIEVLLEVVFTH+
Sbjct: 363  PIFPFDEQKGPCFPCHFFSPTSLYGPSGGAVSAINTMKEMVKKLHASGIEVLLEVVFTHS 422

Query: 1809 VEGGALQGIDDSSYYMNEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFINS 1988
             E GALQGIDDSSYY+  VA+L  RN LNCNYPIVQQ I+DSLR+WV EF VDGFCFIN+
Sbjct: 423  AESGALQGIDDSSYYLKGVADLEARNVLNCNYPIVQQFILDSLRYWVTEFHVDGFCFINA 482

Query: 1989 SSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEMNS 2168
            S LL+G  GEYLS PPLVE IAFDPLLSKTKIIAD WDPH+   ++ RFPHW  WAEMN 
Sbjct: 483  SLLLQGSRGEYLSHPPLVEAIAFDPLLSKTKIIADCWDPHNTVLEETRFPHWKTWAEMNM 542

Query: 2169 RFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFSNT 2348
            +FCNDVR FL+G+ LLS LATRLCGSGDIFS  RGPAFSFNFI++NSGLPLVDLVSFS+ 
Sbjct: 543  KFCNDVRNFLRGEGLLSSLATRLCGSGDIFSKGRGPAFSFNFISRNSGLPLVDLVSFSSD 602

Query: 2349 KLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGSLA 2528
            KLASELSWNCGEEGPTNK  VLERRLKQ RN+LFVLY+SLGVP+LNMGDECGQS GGS A
Sbjct: 603  KLASELSWNCGEEGPTNKTLVLERRLKQTRNFLFVLYVSLGVPVLNMGDECGQSTGGSPA 662

Query: 2529 YSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSELK 2708
            YSDRK FDWNALK+ FGIQTT+FISFL+SLR RR DLLQ  N+LK ENIDWHGSDQS  +
Sbjct: 663  YSDRKSFDWNALKSSFGIQTTEFISFLSSLRRRRSDLLQNMNYLKEENIDWHGSDQSPPR 722

Query: 2709 WDDPSSKFLAMTLKA----DIAESERNPMWGDLFIAINAADCSECVILPPPPEGMAWLRL 2876
            W+DP+ KFLAM L+     D AE++     GDLFIA NAAD SE VILPP  EGMAW RL
Sbjct: 723  WEDPTCKFLAMRLRVDEDKDKAENQTTSGKGDLFIAFNAADLSESVILPPIAEGMAWHRL 782

Query: 2877 VDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFETCS 3002
            VDTALPFPGFF T+GEPV E   GL AYEM S S TLFE  S
Sbjct: 783  VDTALPFPGFFLTDGEPVPENVDGLLAYEMKSLSSTLFEARS 824


>ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like [Fragaria vesca subsp.
            vesca]
          Length = 868

 Score = 1105 bits (2858), Expect = 0.0
 Identities = 556/879 (63%), Positives = 662/879 (75%), Gaps = 15/879 (1%)
 Frame = +3

Query: 411  SVAIRPHCVNCGAT-ESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVA---QKFTKNPLR 578
            S+  +  C  CG T ES KL     Y    K   GL K+   RK       Q F ++ LR
Sbjct: 4    SITPQACCWACGTTSESSKLTANTHYRDRKKTTLGLVKLDAERKLVFGGFGQSFAQSSLR 63

Query: 579  NLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNIKYAVY 758
              + ++ A S +S+   EQ   T TE  E  +  +YLFRTE G  + VFV    + Y+VY
Sbjct: 64   GCHSRVQAASGVSIEPMEQNFPTGTE--ETNKVSTYLFRTENGDLINVFVRDNTVNYSVY 121

Query: 759  IEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSSGIFTT 938
            +E+SSL   ++   L++SWG++R+DSS L P+DF           IETPFT+ SSG FT 
Sbjct: 122  VELSSLQLSSAGDRLVISWGMYRADSSSLKPLDF-----------IETPFTKTSSGSFTL 170

Query: 939  DLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGLSFRVDG 1118
            +L FEAKQTP YLSF +KS  D + +  EIRSHRKTNFCVPVGFG G P PLGLS+  DG
Sbjct: 171  ELEFEAKQTPFYLSFTVKSLADANLSGLEIRSHRKTNFCVPVGFGRGCPTPLGLSYSSDG 230

Query: 1119 SSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNFVSYGYR 1298
            S NFAIFSRNAE V+LCLYDD  A +PALE+DLDPYVNRSGDIWHAS E    F+SYGYR
Sbjct: 231  SINFAIFSRNAESVVLCLYDDPTAQEPALELDLDPYVNRSGDIWHASFESAWTFLSYGYR 290

Query: 1299 FRGEG----DEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAFDWSGDVRP 1466
            F+G      +  D   V+LDPYAK+I  +++++ G+G  +K LG++  EP FDW GDVRP
Sbjct: 291  FKGTSLRNTNSLDEGNVLLDPYAKVIDESIANNRGTG--LKLLGRLCEEPVFDWDGDVRP 348

Query: 1467 NLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLLEPIFAFD 1646
             LP+EKLVVYRLN+  FT+ KSS+LP NVAGTFSGLT+KL HF+DLGVNA+LLEPIF FD
Sbjct: 349  LLPIEKLVVYRLNLKRFTEHKSSKLPTNVAGTFSGLTQKLDHFQDLGVNAILLEPIFPFD 408

Query: 1647 EQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTHTVEGGAL 1826
            E+ GPY PCHFFSPMN +GP+ G +++INSMKEMVK  HA+GIEV+LEV+FTHT EG  L
Sbjct: 409  EEKGPYFPCHFFSPMNCFGPSGGPIASINSMKEMVKEFHANGIEVILEVIFTHTAEGEVL 468

Query: 1827 QGIDDSSYY-MNEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFINSSSLLR 2003
            QGID SSYY  +   +L   N+LNCN+P+VQQM++DSLR+WV EF +DGFCFIN+SSLL 
Sbjct: 469  QGIDISSYYHASTTGDLEAGNSLNCNHPVVQQMVLDSLRYWVTEFHIDGFCFINASSLLH 528

Query: 2004 GFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEMNSRFCND 2183
            G  GEY SRPPLVE I FDPLL+KTK IAD WDPH+M PK+ RFPHW RWAE+N+RFCND
Sbjct: 529  GVKGEYQSRPPLVEAITFDPLLAKTKFIADCWDPHEMVPKETRFPHWKRWAEVNTRFCND 588

Query: 2184 VRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFSNTKLASE 2363
            VR FL+G+ LLSDLATRLCG+GDIFSD RGPAFSFNFI +NSGLPLVDLVSFS ++LASE
Sbjct: 589  VRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFSFNFITRNSGLPLVDLVSFSGSELASE 648

Query: 2364 LSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGSLAYSDRK 2543
            LSWNCGEEGPT+K AVLERRLKQIRN+LF+LY+SLGVP+LNMGDECGQS GGS AYSDRK
Sbjct: 649  LSWNCGEEGPTSKTAVLERRLKQIRNFLFILYLSLGVPVLNMGDECGQSSGGSPAYSDRK 708

Query: 2544 PFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSELKWDDPS 2723
             FDW AL+TGF  Q TQFI++L+SLR RR DLLQK++F K ENIDW+GSDQS  +W+DP 
Sbjct: 709  SFDWKALETGFATQITQFIAYLSSLRRRRSDLLQKKHFFKEENIDWYGSDQSLPRWEDPL 768

Query: 2724 SKFLAMTLKADIAESERNP------MWGDLFIAINAADCSECVILPPPPEGMAWLRLVDT 2885
             KFLA+ LKAD  E E         + GDLF+A +AAD SE VILPPP EGMAW RLVDT
Sbjct: 769  CKFLAVRLKADQDEVENQSNSVYAGLRGDLFLAFSAADQSETVILPPPQEGMAWSRLVDT 828

Query: 2886 ALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFETCS 3002
            ALPFPGFFST+GEPV+EQ   L AYEM S+SC LFE  S
Sbjct: 829  ALPFPGFFSTDGEPVIEQMKDLCAYEMKSHSCALFEARS 867


>ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Populus trichocarpa]
            gi|550345605|gb|ERP64619.1| hypothetical protein
            POPTR_0002s22530g [Populus trichocarpa]
          Length = 857

 Score = 1092 bits (2824), Expect = 0.0
 Identities = 563/881 (63%), Positives = 663/881 (75%), Gaps = 14/881 (1%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCV-NCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQKFTKN 569
            M TL P +AI P C  NCG+ ES K I    Y+   K            K+   ++F + 
Sbjct: 1    MTTLSPLLAITPLCFCNCGSVESSKSI---HYVCKTK------------KSRPTRRFGRM 45

Query: 570  PLRNLNFKLLATSQISVTQTEQRLTTSTE--IRELQETLSYLFRTEIGGHVKVFVGKKNI 743
             +   +  L A S+++   TEQ + TS+   + +L+E  +Y FRTEIGGHVK+ VGK N 
Sbjct: 46   DVGKDSRLLFARSRVAAQPTEQMMFTSSNPLVDQLKEVSTYRFRTEIGGHVKISVGKING 105

Query: 744  KYAVYIEVSSL-LRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKS 920
            KYAVY+EVSSL L  + +  L+L WGI+ SDSSC MP+D  + A      T ETP  Q S
Sbjct: 106  KYAVYVEVSSLELGASDNISLMLIWGIYTSDSSCFMPLDSSSHAR-----TRETPLLQNS 160

Query: 921  SGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGL 1100
               F T+L FEAKQTP YLSF LK +    S+  EIR+H K+NFCVP+GF SG P PLGL
Sbjct: 161  CARFATELEFEAKQTPFYLSFFLKPT----SSVVEIRNHNKSNFCVPIGFDSGYPTPLGL 216

Query: 1101 SFRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNF 1280
            SF  DGS NFA FSRNA G +LCLYDD+ + KPALE+DLDPYVNRSGDIWHAS+E    F
Sbjct: 217  SFSTDGSMNFAFFSRNAAGCVLCLYDDSTSGKPALELDLDPYVNRSGDIWHASLEGAWTF 276

Query: 1281 VSYGYRFRG-----EGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAFD 1445
            +SYGYR +G     + D+FD  RV+LDPY+KII N+V+D+  SG   K+LG++ +EP FD
Sbjct: 277  LSYGYRCKGAALQSDADKFDAGRVLLDPYSKIIINSVTDNV-SGLLPKYLGRLCKEPVFD 335

Query: 1446 WSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLL 1625
            WS DV P+L MEKLVVYR+NVM FTKD SSQ+  + AGTF+GL EKL HFK+LGVNAVLL
Sbjct: 336  WSDDVPPHLEMEKLVVYRVNVMRFTKDNSSQISSDAAGTFAGLIEKLSHFKNLGVNAVLL 395

Query: 1626 EPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTH 1805
            E IF FDEQ GPY PCHFFSP N+YGP+NG V+AI+SMKEMVK +HA+GIEVLLEVVFTH
Sbjct: 396  ESIFPFDEQKGPYFPCHFFSPSNVYGPSNGSVAAISSMKEMVKRLHANGIEVLLEVVFTH 455

Query: 1806 TVEGGALQGIDDSSYYMNEVAN-LGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFI 1982
            T E GALQGIDDSSYY   +   L ++NALNCN+PIVQ++I+DSL+HWV EF +DGFCFI
Sbjct: 456  TAEAGALQGIDDSSYYCTSITTGLDSQNALNCNHPIVQRVILDSLQHWVTEFHIDGFCFI 515

Query: 1983 NSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEM 2162
            N+ SLLR F GEYLSRPP+VE IAFDPL SKTKIIAD WDP ++  K+  FPHW +WAEM
Sbjct: 516  NALSLLRSFGGEYLSRPPVVEAIAFDPLFSKTKIIADCWDPKELEAKETAFPHWKKWAEM 575

Query: 2163 NSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFS 2342
            N++FC D+R FL+G+ LLSDLATRLCGSGDIFS  RGPAFSFNFI +N GLPLVDLVSFS
Sbjct: 576  NTKFCYDIRNFLRGEGLLSDLATRLCGSGDIFSSGRGPAFSFNFITRNFGLPLVDLVSFS 635

Query: 2343 NTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGS 2522
              +LASELSWNCGEEGPTNK  +LERRLKQIRNYLFVL++SLGVP+LNMGDECGQS GGS
Sbjct: 636  GDELASELSWNCGEEGPTNKTTILERRLKQIRNYLFVLFVSLGVPVLNMGDECGQSSGGS 695

Query: 2523 LAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSE 2702
             +Y  RKPFDWNAL TGFGIQTTQFISFL+SLR+RR DLLQKRNFLK ENIDWHGSDQ+ 
Sbjct: 696  TSYGSRKPFDWNALSTGFGIQTTQFISFLSSLRMRRSDLLQKRNFLKEENIDWHGSDQNP 755

Query: 2703 LKWDDPSSKFLAMTLKADI----AESERNPMWGDLFIAINAADCSECVILPPPPEGMAWL 2870
             +W+DPS KFLAMTLK D       SE + + GD+FIA NAA  SE V LP  PEGMAW 
Sbjct: 756  PRWEDPSCKFLAMTLKVDNPGSGLSSESSHVEGDMFIAFNAAGRSESVTLPEVPEGMAWH 815

Query: 2871 RLVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
            RLVDTALPFPGFFS + EPV+ Q      YEM S+SC L E
Sbjct: 816  RLVDTALPFPGFFSNDSEPVIRQ-----PYEMKSHSCILLE 851


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Cucumis sativus]
          Length = 885

 Score = 1048 bits (2710), Expect = 0.0
 Identities = 531/886 (59%), Positives = 656/886 (74%), Gaps = 16/886 (1%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCV-NCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQK---F 560
            MAT L S+ I+P CV NCG TESPKL        G K      K+   R     +     
Sbjct: 1    MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 561  TKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKN 740
             K+  RNL+ K  A S ISV ++ QRL    + +E +   +YLFRTE G  V VFVGKK 
Sbjct: 61   VKSSHRNLS-KTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKG 119

Query: 741  IKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKS 920
              + V IEV S+  ++ D  L+LSWG++RSDS+ + P +F++S     TG  ETPF + S
Sbjct: 120  STFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTS 178

Query: 921  SGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGL 1100
             G F+ +L F+AK TP YLSF+LK  + VDS SSEIRSH+KT+F VPVGFG G P+PLGL
Sbjct: 179  EGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGL 238

Query: 1101 SFRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNF 1280
            S   DGS NF+IFS +AE ++LCLY+D+ ++KP LE+DLDPY+NRSG+IWHAS E    F
Sbjct: 239  SISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 298

Query: 1281 VSYGYRFRGEG-----DEFDTERVILDPYAKIIGNAV--SDHHGSGFPVKHLGQVSREPA 1439
            VSYGY+ +G       D  +  R+++DPYAKI+  ++  S   G G P K LGQ+S+ P 
Sbjct: 299  VSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPT 358

Query: 1440 FDWSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAV 1619
            FDW G+V PNLPMEKL VYRLNV  FT DKSSQLP ++AGTFSGLT+KL HFK+LGVNAV
Sbjct: 359  FDWDGEVHPNLPMEKLXVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV 418

Query: 1620 LLEPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVF 1799
            LLEPIF FDE+ GPY P HFFSP N YGP+   +SAINSMKEMVK +HA+G+EV+LEVV+
Sbjct: 419  LLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVY 478

Query: 1800 THTVEGGALQGIDDSSYYM-NEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFC 1976
            THT   GALQGIDDSSYY  N VANL  ++ALNCNYPIVQQ+++DSLR+WV EF VDGFC
Sbjct: 479  THTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFC 538

Query: 1977 FINSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWA 2156
            F+N+S LLRG HGE LSRPP VE IAFDPLLSKTK++AD WDP ++  K+ RFPHW RWA
Sbjct: 539  FVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWA 598

Query: 2157 EMNSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVS 2336
            E+NS+FC+D+R F +G+ L+S LATRLCGSGD+FSD RGPAFSFNFIA+N GLPLVDLVS
Sbjct: 599  EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS 658

Query: 2337 FSNTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYG 2516
            FSN+ LASELSWNCGEEGPT+   VLE+RLKQIRN++FVL++SLGVP+LNMGDECGQS G
Sbjct: 659  FSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSG 718

Query: 2517 GSLAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQ 2696
            GS+A++D++ F+W+ LKT FG QTTQFI+FL+S R RR DL Q RNFLK ENIDW  ++Q
Sbjct: 719  GSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQ 778

Query: 2697 SELKWDDPSSKFLAMTLKADIAESE---RNPMW-GDLFIAINAADCSECVILPPPPEGMA 2864
            S  +W+D S KFLA+ L+AD  E+E    NP    ++F+  NA+D SE V LP P EG +
Sbjct: 779  SPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTS 838

Query: 2865 WLRLVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFETCS 3002
            W R+VDTALPFPGFFS++GE  L    G   YE+ ++SC LFE  S
Sbjct: 839  WFRVVDTALPFPGFFSSDGE--LVPMTGSVTYEIQAHSCALFEAKS 882


>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
          Length = 885

 Score = 1048 bits (2710), Expect = 0.0
 Identities = 531/886 (59%), Positives = 656/886 (74%), Gaps = 16/886 (1%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCV-NCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQK---F 560
            MAT L S+ I+P CV NCG TESPKL        G K      K+   R     +     
Sbjct: 1    MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 561  TKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKN 740
             K+  RNL+ K  A S ISV ++ QRL    + +E +   +YLFRTE G  V VFVGKK 
Sbjct: 61   VKSSHRNLS-KTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKG 119

Query: 741  IKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKS 920
              + V IEV S+  ++ D  L+LSWG++RSDS+ + P +F++S     TG  ETPF + S
Sbjct: 120  STFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTS 178

Query: 921  SGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGL 1100
             G F+ +L F+AK TP YLSF+LK  + VDS SSEIRSH+KT+F VPVGFG G P+PLGL
Sbjct: 179  EGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGL 238

Query: 1101 SFRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNF 1280
            S   DGS NF+IFS +AE ++LCLY+D+ ++KP LE+DLDPY+NRSG+IWHAS E    F
Sbjct: 239  SISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 298

Query: 1281 VSYGYRFRGEG-----DEFDTERVILDPYAKIIGNAV--SDHHGSGFPVKHLGQVSREPA 1439
            VSYGY+ +G       D  +  R+++DPYAKI+  ++  S   G G P K LGQ+S+ P 
Sbjct: 299  VSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPT 358

Query: 1440 FDWSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAV 1619
            FDW G+V PNLPMEKL VYRLNV  FT DKSSQLP ++AGTFSGLT+KL HFK+LGVNAV
Sbjct: 359  FDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV 418

Query: 1620 LLEPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVF 1799
            LLEPIF FDE+ GPY P HFFSP N YGP+   +SAINSMKEMVK +HA+G+EV+LEVV+
Sbjct: 419  LLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVY 478

Query: 1800 THTVEGGALQGIDDSSYYM-NEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFC 1976
            THT   GALQGIDDSSYY  N VANL  ++ALNCNYPIVQQ+++DSLR+WV EF VDGFC
Sbjct: 479  THTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFC 538

Query: 1977 FINSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWA 2156
            F+N+S LLRG HGE LSRPP VE IAFDPLLSKTK++AD WDP ++  K+ RFPHW RWA
Sbjct: 539  FVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWA 598

Query: 2157 EMNSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVS 2336
            E+NS+FC+D+R F +G+ L+S LATRLCGSGD+FSD RGPAFSFNFIA+N GLPLVDLVS
Sbjct: 599  EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS 658

Query: 2337 FSNTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYG 2516
            FSN+ LASELSWNCGEEGPT+   VLE+RLKQIRN++FVL++SLGVP+LNMGDECGQS G
Sbjct: 659  FSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSG 718

Query: 2517 GSLAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQ 2696
            GS+A++D++ F+W+ LKT FG QTTQFI+FL+S R RR DL Q RNFLK ENIDW  ++Q
Sbjct: 719  GSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQ 778

Query: 2697 SELKWDDPSSKFLAMTLKADIAESE---RNPMW-GDLFIAINAADCSECVILPPPPEGMA 2864
            S  +W+D S KFLA+ L+AD  E+E    NP    ++F+  NA+D SE V LP P EG +
Sbjct: 779  SPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTS 838

Query: 2865 WLRLVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFETCS 3002
            W R+VDTALPFPGFFS++GE  L    G   YE+ ++SC LFE  S
Sbjct: 839  WFRVVDTALPFPGFFSSDGE--LVPMTGSVTYEIQAHSCALFEAKS 882


>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 865

 Score = 1032 bits (2668), Expect = 0.0
 Identities = 526/824 (63%), Positives = 628/824 (76%), Gaps = 12/824 (1%)
 Frame = +3

Query: 558  FTKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGH-VKVFVGK 734
            F+ NP  +   KL ATS++S+ +TEQ++ T T   +L+ +L+YLFRTE GG  VKV V +
Sbjct: 48   FSLNPTSS---KLCATSRLSIEETEQQIGTLTRPEDLKGSLAYLFRTETGGGLVKVHVTR 104

Query: 735  KNIKYAVYIEVSSLLRLNSDSE-LILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFT 911
            +N +Y+VYIE+SSL       E L+L WG++RSDSSC + +D    +  A  G   +P  
Sbjct: 105  RNDRYSVYIEISSLDISGGVGEALLLCWGVYRSDSSCFVDLDTIGLSENAAMGMNVSPLV 164

Query: 912  QKSSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAP 1091
            Q S G F  +L F+AK  PLYLSF L SSLD   +  EIRSHR+TNFC+PVG   G P P
Sbjct: 165  QNSDGKFAIELEFDAKHVPLYLSFFLMSSLD---SGLEIRSHRRTNFCMPVGSLPGYPCP 221

Query: 1092 LGLSFRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEIL 1271
            LG+S+  DGS NF+IFSR+AE V+LCLYD+   +KPALE+DLDPYVNR+GDIWH S E  
Sbjct: 222  LGVSYSPDGSVNFSIFSRHAESVVLCLYDENGVEKPALELDLDPYVNRTGDIWHVSFESA 281

Query: 1272 PNFVSYGYRFRG-----EGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREP 1436
              FVSYGYR R        D+   E V+LDPYAKI+GN+  D  G    VK+LG + +EP
Sbjct: 282  KGFVSYGYRCRRGVLKKNKDDGFAEHVVLDPYAKIVGNSYPDGVGL---VKNLGCLRKEP 338

Query: 1437 AFDWSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNA 1616
             FDW GD  P+L MEKLVVYRLNV  FT+ +SSQLP  +AGTF+GL +K+ HFKDLGVNA
Sbjct: 339  FFDWGGDRHPDLSMEKLVVYRLNVKRFTQHESSQLPSGLAGTFTGLAKKVQHFKDLGVNA 398

Query: 1617 VLLEPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVV 1796
            VLLEP+F FDE+ GPY PCHFFS M++YGP+ G VSAI +MKEMVKTMHA+GIEVL+EVV
Sbjct: 399  VLLEPVFTFDEKKGPYFPCHFFSLMHIYGPSGGPVSAIAAMKEMVKTMHANGIEVLVEVV 458

Query: 1797 FTHTVEGGALQGIDDSSYYM-NEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGF 1973
            F++T E GA+QGIDDSSYY  N V  L  ++ALNCNYPIVQ +I+DSLRHWV EF +DGF
Sbjct: 459  FSNTAEIGAIQGIDDSSYYYANGVGGLKVQSALNCNYPIVQNLILDSLRHWVTEFHIDGF 518

Query: 1974 CFINSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRW 2153
             FIN+S LLRGFHGEYLSRPPLVE IAFDP+LSKTKIIAD WDPH M  K+IRFPHWMRW
Sbjct: 519  SFINASHLLRGFHGEYLSRPPLVEAIAFDPVLSKTKIIADCWDPHGMVAKEIRFPHWMRW 578

Query: 2154 AEMNSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLV 2333
            AEMN+ FCNDVR FL+G  LLSDLATRLCGSGDIFS  RGP FSFN+IA+N G+ LVDLV
Sbjct: 579  AEMNTHFCNDVRNFLRGQNLLSDLATRLCGSGDIFSGGRGPGFSFNYIARNFGVSLVDLV 638

Query: 2334 SFSNTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSY 2513
            SFS+     ELSWNCG EGPTN  AVLERRLKQIRN+LF+L++SLGVP+LNMGDECGQS 
Sbjct: 639  SFSS---VDELSWNCGAEGPTNNTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSS 695

Query: 2514 GGSLAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSD 2693
            GG  AY   KPF W+ALKTGFG QT++FI FL+SLR RR  LLQ+R+FLK ENI+W+GSD
Sbjct: 696  GGFTAYDGIKPFSWSALKTGFGKQTSEFIFFLSSLRKRRSYLLQRRSFLKEENIEWYGSD 755

Query: 2694 QSELKWDDPSSKFLAMTLKADIAE----SERNPMWGDLFIAINAADCSECVILPPPPEGM 2861
                +W+DPS KFLAM LKA++ E    S  + + GDLFIA NA D  E  +LP PPEGM
Sbjct: 756  GDPPRWEDPSCKFLAMILKAEVTEFLESSVSSDISGDLFIAFNATDHPETAVLPLPPEGM 815

Query: 2862 AWLRLVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
            +W RLVDTALPFPGFFST+GE V EQT GL+ Y++ SYSCTLFE
Sbjct: 816  SWYRLVDTALPFPGFFSTSGEVVPEQTEGLFTYQVKSYSCTLFE 859


>ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Solanum lycopersicum]
          Length = 878

 Score = 1022 bits (2642), Expect = 0.0
 Identities = 517/876 (59%), Positives = 635/876 (72%), Gaps = 9/876 (1%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIH---MGRKTEVAQKFT 563
            M T    +A+    ++ G+TES KL+ PA      K +C L K+    M           
Sbjct: 1    METSPIQLAVHSRLLSYGSTESTKLV-PASSGNRGKIVCSLRKLELEDMNFSGISRNNDQ 59

Query: 564  KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 743
            + P R    K L+ S+IS+  + +R+ T            YLFRT+IG  VKVFV K N 
Sbjct: 60   EAPRRAHRLKALSGSRISLVPSAKRVPT------------YLFRTDIGCQVKVFVEKTNG 107

Query: 744  KYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSS 923
            KY V +EV  L   ++ SEL++ WG+FRSD+SC MP+D        ++ T+ETPF Q  S
Sbjct: 108  KYKVLVEVLPLELSDAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPS 167

Query: 924  GIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGLS 1103
            G  T +L FE    P Y+SF +KS L  D  +SEIRSHR TNF VPVG  SG PAPLG+S
Sbjct: 168  GKVTVELDFEVSLVPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGIS 227

Query: 1104 FRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNFV 1283
            F+ DG  NFA++SR+A+GV+LCLYDD   +KP+LEIDLDPY+N+SGDIWHA+++    F 
Sbjct: 228  FQPDGYVNFALYSRSAKGVVLCLYDDISVEKPSLEIDLDPYINQSGDIWHAALDCSLPFK 287

Query: 1284 SYGYRFR----GEGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAFDWS 1451
            +YGYRF+    G+G     E V+LDPYAK+I + +    GS    K+LG++  EP +DWS
Sbjct: 288  TYGYRFKAATSGKG-----ELVLLDPYAKVIRSVIPRQGGSEIRPKYLGELCLEPGYDWS 342

Query: 1452 GDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLLEP 1631
            GDV P+LPMEKL++YR+NV HFTKDKSS+LP N+AGTFSG++EK HH KDLGVNA+LLEP
Sbjct: 343  GDVPPSLPMEKLIIYRINVTHFTKDKSSKLPDNLAGTFSGISEKWHHLKDLGVNAMLLEP 402

Query: 1632 IFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTHTV 1811
            IF FDEQ GPY P HFFSP N+YGP+   +S I SMK+MVK +HA+GIEV LEVVFTHT 
Sbjct: 403  IFPFDEQKGPYYPWHFFSPGNMYGPSGDPLSVIKSMKDMVKKLHANGIEVFLEVVFTHTA 462

Query: 1812 EGGALQGIDDSSYYMNEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFINSS 1991
            E   L  +D+ SY +    ++  +NALNCNYPIVQQMI+D LRHWVIEF +DGF F+++S
Sbjct: 463  EDAPLMNVDNFSYCIKGSQDMNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVDAS 522

Query: 1992 SLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEMNSR 2171
            SLLRGF+GE LSRPPLVE IAFDP+LSK K+IADNW+P      +  FPHW RWAE+N R
Sbjct: 523  SLLRGFNGEILSRPPLVEAIAFDPILSKAKMIADNWNPLTNDSTENLFPHWRRWAEINMR 582

Query: 2172 FCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFSNTK 2351
            FC+D+R FL+G+ LLS+LATRLCGSGDIF+  RGPAFSFN+IA+NSGL LVDLVSFSN++
Sbjct: 583  FCDDIRDFLRGEGLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSNSE 642

Query: 2352 LASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGSLAY 2531
            +ASELSWNCG+EG T    VLERRLKQ+RN+LF+L+ISLGVP+LNMGDECGQS GGS AY
Sbjct: 643  VASELSWNCGQEGATTNSIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPAY 702

Query: 2532 SDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSELKW 2711
              RK  +WN LKTGFG Q  QFISFL++LR+RR DLLQKRNFLK ENI WHGSDQS   W
Sbjct: 703  DARKSLNWNTLKTGFGTQIAQFISFLSNLRMRRSDLLQKRNFLKEENIQWHGSDQSPPVW 762

Query: 2712 DDPSSKFLAMTLKAD--IAESERNPMWGDLFIAINAADCSECVILPPPPEGMAWLRLVDT 2885
            DDPSSKFLAMTLKAD  ++ +  + + GDLF+A N A  SE VILPPPP  M W RLVDT
Sbjct: 763  DDPSSKFLAMTLKADAEVSHTLLSDIGGDLFVAFNGAGDSESVILPPPPTDMVWYRLVDT 822

Query: 2886 ALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
            ALPFPGFF   G PV ++   L AYEM S+SC LFE
Sbjct: 823  ALPFPGFFDEKGTPVEDE---LVAYEMKSHSCVLFE 855


>ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Solanum tuberosum]
          Length = 878

 Score = 1019 bits (2636), Expect = 0.0
 Identities = 521/881 (59%), Positives = 635/881 (72%), Gaps = 14/881 (1%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHM--------GRKTEV 548
            MAT    +A+    ++ G+TES KL+ P+      K +C L K+ +        GR  + 
Sbjct: 1    MATSPIQLAVHSRLLSYGSTESTKLV-PSSSGNRGKIVCSLRKLELEDMNFSGIGRNND- 58

Query: 549  AQKFTKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFV 728
                 + P R    K L+ S+IS+  + +R+ T            YLFRT+IGG VKV V
Sbjct: 59   ----QEAPRRAHRRKALSASRISLVPSAKRVPT------------YLFRTDIGGQVKVLV 102

Query: 729  GKKNIKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPF 908
             + N KY V +EV  L    + SEL++ WG+FRSD+SC MP+D        ++ T+ETPF
Sbjct: 103  ERTNGKYKVLVEVLPLELSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPF 162

Query: 909  TQKSSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPA 1088
             Q  SG  T +L FEA   P Y+SF +KS L  D  +SEIRSHR TNF VPVG  SG PA
Sbjct: 163  VQGPSGKVTVELDFEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPA 222

Query: 1089 PLGLSFRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEI 1268
            PLG+SF+ DGS NFA+FSR+A  V+LCLYDD   +KP+LEIDLDPY+NRSGDIWHA+++ 
Sbjct: 223  PLGISFQPDGSVNFALFSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDC 282

Query: 1269 LPNFVSYGYRFR----GEGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREP 1436
               F +YGYR +    G+G     E V+LDPYAK+I   +    GS    K+LG++  EP
Sbjct: 283  SLPFKTYGYRCKATTSGKG-----ELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEP 337

Query: 1437 AFDWSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNA 1616
             +DWSGDV P+LPMEKL++YRLNV  FTKDKSS+LP ++AGTFSG++EK HHFKDLGVNA
Sbjct: 338  GYDWSGDVPPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNA 397

Query: 1617 VLLEPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVV 1796
            +LLEPIF FDEQ GPY P HFFSP N+YGP+   +SAI SMK+MVK +HA+GIEV LEVV
Sbjct: 398  MLLEPIFPFDEQKGPYFPWHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVV 457

Query: 1797 FTHTVEGGALQGIDDSSYYMNEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFC 1976
            FTHT E   L  +D+ SY +     L  +NALNCNYPIVQQMI+D LRHWVIEF +DGF 
Sbjct: 458  FTHTAEDAPLMNVDNFSYCIKGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFV 517

Query: 1977 FINSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWA 2156
            F+N+SSLLRGF+GE LSRPPLVE IAFDP+LSK K+IADNW+P     K+  FPHW RWA
Sbjct: 518  FVNASSLLRGFNGEILSRPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHWRRWA 577

Query: 2157 EMNSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVS 2336
            E+N RFC+D+R FL+G+ LLS+LATRLCGSGDIF+  RGPAFSFN+IA+NSGL LVDLVS
Sbjct: 578  EINMRFCDDIRDFLRGEGLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVS 637

Query: 2337 FSNTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYG 2516
            FS+ ++ASELSWNCG+EG T    VLERRLKQ+RN+LF+L+ISLGVP+LNMGDECGQS G
Sbjct: 638  FSSNEVASELSWNCGQEGATTNNIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSG 697

Query: 2517 GSLAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQ 2696
            GS AY  RK   WN LKTGFG Q  QFISFL++LR+RR DLLQKR FLK ENI WHGSDQ
Sbjct: 698  GSPAYDARKSLGWNTLKTGFGTQIAQFISFLSNLRMRRSDLLQKRTFLKEENIQWHGSDQ 757

Query: 2697 SELKWDDPSSKFLAMTLKAD--IAESERNPMWGDLFIAINAADCSECVILPPPPEGMAWL 2870
            S  KWD PSSKFLAMTLKAD  ++++  + + GDLF+A N A  SE VILPPPP  M W 
Sbjct: 758  SPPKWDGPSSKFLAMTLKADAEVSQTLVSDIVGDLFVAFNGAGDSEIVILPPPPTDMVWH 817

Query: 2871 RLVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
            RLVDTALPFPGFF   G PV ++   L AYEM S+SC LFE
Sbjct: 818  RLVDTALPFPGFFDEKGTPVEDE---LVAYEMKSHSCLLFE 855


>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|27728147|gb|AAN15318.1|
            isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score = 1018 bits (2631), Expect = 0.0
 Identities = 520/881 (59%), Positives = 634/881 (71%), Gaps = 14/881 (1%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHM--------GRKTEV 548
            MAT    +A+    ++ G+TES KL+ P+      K +C L K+ +        GR  + 
Sbjct: 1    MATSPIQLAVHSRLLSYGSTESTKLV-PSSSGNRGKIVCSLRKLELEDMNFSGIGRNND- 58

Query: 549  AQKFTKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFV 728
                 + P R    K L+ S+IS+  + +R+ T            YLFRT+IGG VKV V
Sbjct: 59   ----QEAPRRAHRRKALSASRISLVPSAKRVPT------------YLFRTDIGGQVKVLV 102

Query: 729  GKKNIKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPF 908
             + N KY V +EV  L    + SEL++ WG+FRSD+SC MP+D        ++ T+ETPF
Sbjct: 103  ERTNGKYKVLVEVLPLELSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPF 162

Query: 909  TQKSSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPA 1088
             Q  SG  T +L FEA   P Y+SF +KS L  D  +SEIRSHR TNF VPVG  SG PA
Sbjct: 163  VQGPSGKVTVELDFEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPA 222

Query: 1089 PLGLSFRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEI 1268
            PLG+SF+ DGS NFA+FSR+A  V+LCLYDD   +KP+LEIDLDPY+NRSGDIWHA+++ 
Sbjct: 223  PLGISFQPDGSVNFALFSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDC 282

Query: 1269 LPNFVSYGYRFR----GEGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREP 1436
               F +YGYR +    G+G     E V+LDPYAK+I   +    GS    K+LG++  EP
Sbjct: 283  SLPFKTYGYRCKATTSGKG-----ELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEP 337

Query: 1437 AFDWSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNA 1616
             +DWSGDV P+LPMEKL++YRLNV  FTKDKSS+LP ++AGTFSG++EK HHFKDLGVNA
Sbjct: 338  GYDWSGDVPPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNA 397

Query: 1617 VLLEPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVV 1796
            +LLEPIF FDEQ GPY P HFFSP N+YGP+   +SAI SMK+MVK +HA+GIEV LEVV
Sbjct: 398  MLLEPIFPFDEQKGPYFPWHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVV 457

Query: 1797 FTHTVEGGALQGIDDSSYYMNEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFC 1976
            FTHT E   L  +D+ SY +     L  +NALNCNYPIVQQMI+D LRHWVIEF +DGF 
Sbjct: 458  FTHTAEDAPLMNVDNFSYCIKGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFV 517

Query: 1977 FINSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWA 2156
            F+N+SSLLRGF+GE LSRPPLVE IAFDP+LSK K+IADNW+P     K+  FPHW RWA
Sbjct: 518  FVNASSLLRGFNGEILSRPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHWRRWA 577

Query: 2157 EMNSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVS 2336
            E+N RFC+D+R FL+G+ LLS+LATRLCGSGDIF+  RGPAFSFN+IA+NSGL LVDLVS
Sbjct: 578  EINMRFCDDIRDFLRGEGLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVS 637

Query: 2337 FSNTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYG 2516
            FS+ ++ASELSWNCG+EG T    VLERRLKQ+RN+LF+L+ISLGVP+LNMGDECGQS G
Sbjct: 638  FSSNEVASELSWNCGQEGATTNNIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSG 697

Query: 2517 GSLAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQ 2696
            G  AY  RK   WN LKTGFG Q  QFISFL++LR+RR DLLQKR FLK ENI WHGSDQ
Sbjct: 698  GPPAYDARKSLGWNTLKTGFGTQIAQFISFLSNLRMRRSDLLQKRTFLKEENIQWHGSDQ 757

Query: 2697 SELKWDDPSSKFLAMTLKAD--IAESERNPMWGDLFIAINAADCSECVILPPPPEGMAWL 2870
            S  KWD PSSKFLAMTLKAD  ++++  + + GDLF+A N A  SE VILPPPP  M W 
Sbjct: 758  SPPKWDGPSSKFLAMTLKADAEVSQTLVSDIVGDLFVAFNGAGDSEIVILPPPPTDMVWH 817

Query: 2871 RLVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
            RLVDTALPFPGFF   G PV ++   L AYEM S+SC LFE
Sbjct: 818  RLVDTALPFPGFFDEKGTPVEDE---LVAYEMKSHSCLLFE 855


>ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 866

 Score = 1015 bits (2625), Expect = 0.0
 Identities = 522/824 (63%), Positives = 621/824 (75%), Gaps = 12/824 (1%)
 Frame = +3

Query: 558  FTKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGH-VKVFVGK 734
            F++NP  +   KL ATS++S+ +TEQ+L T +    L+ + +YLFRTE GG  VKV+V K
Sbjct: 49   FSRNPTSS---KLCATSRLSIEETEQQLGTLSRPEYLKGSSAYLFRTETGGGLVKVYVTK 105

Query: 735  KNIKYAVYIEVSSLLRLNSDSE-LILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFT 911
            +  +Y VY E+SSL    S  E L+L WG++RSDSSC + +D       A TG   +P  
Sbjct: 106  RKDRYFVYTEISSLDISGSVGETLLLCWGVYRSDSSCFVDLDTIGLRENAATGMNVSPLV 165

Query: 912  QKSSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAP 1091
            Q S G F  +L F+AK  PLYLSF L SSLD      EIRSHR TNFCVPVG   G P P
Sbjct: 166  QNSDGNFAVELEFDAKHVPLYLSFFLMSSLDA---GMEIRSHRGTNFCVPVGLLPGYPGP 222

Query: 1092 LGLSFRVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEIL 1271
            LGL    DGS NFAIFSR AE V+LCLYD+   +KPALE+DLDPYVNR+GDIWH + E  
Sbjct: 223  LGLFCSPDGSVNFAIFSRRAESVVLCLYDENDMEKPALELDLDPYVNRTGDIWHVAFESA 282

Query: 1272 PNFVSYGYRFRG-----EGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREP 1436
              F+SYGY  RG       D+   E V+LDPYAKI+GN+  D  G GF VK+LG + +EP
Sbjct: 283  KGFMSYGYSCRGGVLKRNKDDGFAEHVVLDPYAKIVGNSYPD--GVGF-VKNLGWLGKEP 339

Query: 1437 AFDWSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNA 1616
             FDW GD   +L MEKLVVYRLNV  FT+ +SSQLP  +AGTF+GL +K+ HFKDLGVNA
Sbjct: 340  DFDWGGDCHLDLSMEKLVVYRLNVKRFTQHESSQLPSGLAGTFTGLAKKVQHFKDLGVNA 399

Query: 1617 VLLEPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVV 1796
            +LLEP+F FDE+ GPY P HFFS M++YGP+ G VSAI SMKEMVKTMHA+GIEVL+EVV
Sbjct: 400  ILLEPVFTFDEKKGPYFPSHFFSLMHIYGPSGGPVSAIASMKEMVKTMHANGIEVLVEVV 459

Query: 1797 FTHTVEGGALQGIDDSSYYM-NEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGF 1973
            F++T E GALQGIDDS+YY  N V  L  ++ALNCNYPIVQ +I+DSLRHWV EF +DGF
Sbjct: 460  FSNTAEIGALQGIDDSTYYYANGVGGLKGQSALNCNYPIVQSLILDSLRHWVTEFHIDGF 519

Query: 1974 CFINSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRW 2153
             F+N+S LLRGFHGEYL+RPPLVE IAFDP+LSKTKIIAD WDPH M  K+IRFPHWMRW
Sbjct: 520  SFLNASHLLRGFHGEYLTRPPLVEAIAFDPVLSKTKIIADCWDPHGMVAKEIRFPHWMRW 579

Query: 2154 AEMNSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLV 2333
            AE+N+ FCNDVR FL+G+ LLS+LATRLCGSGDIFS  RGPAFSFN+IA+N G+ LVDLV
Sbjct: 580  AEINTNFCNDVRNFLRGENLLSNLATRLCGSGDIFSGGRGPAFSFNYIARNFGVSLVDLV 639

Query: 2334 SFSNTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSY 2513
            SFS+T    ELSWNCGEEGPTN  A+LERRLKQIRN+LF+L++SLGVP+LNMGDECGQS 
Sbjct: 640  SFSST---DELSWNCGEEGPTNNTAILERRLKQIRNFLFILFVSLGVPVLNMGDECGQSS 696

Query: 2514 GGSLAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSD 2693
            GG  AY   KPF W++LKTGFG QT+QFI FL+S R RR DLLQ+ +FLK ENI+W+GSD
Sbjct: 697  GGFTAYDGIKPFSWSSLKTGFGKQTSQFIFFLSSFRRRRSDLLQRMSFLKEENIEWYGSD 756

Query: 2694 QSELKWDDPSSKFLAMTLKAD----IAESERNPMWGDLFIAINAADCSECVILPPPPEGM 2861
             +  +W+D S KFLAM LKA+    +  S  + + GDLFIA NAA   E  +LP PPEGM
Sbjct: 757  GAPPRWEDLSCKFLAMALKAEEKEFLESSVSSDISGDLFIAFNAAGHPETAVLPLPPEGM 816

Query: 2862 AWLRLVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
             W RLVDTALPFPGFFS +GE V EQTAGL+ Y M SYSCTLFE
Sbjct: 817  LWYRLVDTALPFPGFFSASGEVVPEQTAGLFTYRMKSYSCTLFE 860


>ref|XP_007162398.1| hypothetical protein PHAVU_001G148700g [Phaseolus vulgaris]
            gi|139867055|dbj|BAF52942.1| isoamylase-type
            starch-debranching enzyme 2 [Phaseolus vulgaris]
            gi|561035862|gb|ESW34392.1| hypothetical protein
            PHAVU_001G148700g [Phaseolus vulgaris]
          Length = 865

 Score = 1009 bits (2610), Expect = 0.0
 Identities = 517/826 (62%), Positives = 625/826 (75%), Gaps = 15/826 (1%)
 Frame = +3

Query: 576  RNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGH-VKVFVGKKNIKYA 752
            RN + KL ATS++S+ +TEQ++   T   +L+  L+YLFRTE GG  VKV+V KK  +Y 
Sbjct: 50   RNPSSKLCATSRLSIEETEQQVEPFTRPEDLKGALAYLFRTETGGGLVKVYVTKKKDRYF 109

Query: 753  VYIEVSSL-LRLNSDSE-LILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSSG 926
            VYIE+SSL +    DSE L+L WG++R DSSC + +D    +  A      +P  Q S  
Sbjct: 110  VYIEISSLDVNHCGDSETLVLCWGVYRGDSSCFVDMDSTGLSGNAAKRMNVSPLVQTSVC 169

Query: 927  IFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGLSF 1106
             F  +L F+AK  PLYLSF L SSLD      EI SHR+TNFCVPVG   G P PLGLS+
Sbjct: 170  KFGVELEFDAKYVPLYLSFFLMSSLDA---GLEIISHRRTNFCVPVGLLPGYPGPLGLSY 226

Query: 1107 RVDGSSNFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPNFVS 1286
              DGS NFAIFSR+AE V+LCLYD+   +KPALE+DLDPYVNRSGDIWH S E + +FVS
Sbjct: 227  SPDGSVNFAIFSRHAESVVLCLYDEKGVEKPALEVDLDPYVNRSGDIWHVSFESVKSFVS 286

Query: 1287 YGYRFRG------EGDEFDTERVILDPYAKIIGNAVSDHHGSGFP-VKHLGQVSREPAFD 1445
            YGYR RG       GD    E V+LDPYAKI+G++    + SG   V++LG + +EPAFD
Sbjct: 287  YGYRCRGGVHKQNNGDS-SAELVVLDPYAKIVGHS----YPSGLELVQNLGWLGKEPAFD 341

Query: 1446 WSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLL 1625
            W GD  P+L ME+LVVYRLNV  FT+  SSQLP   AGTF+GL EK+ HFKDLGVNAVLL
Sbjct: 342  WGGDFLPDLSMEELVVYRLNVKRFTQHNSSQLPSGSAGTFTGLAEKVQHFKDLGVNAVLL 401

Query: 1626 EPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTH 1805
            EP+F  DE+ GPY PCHFFS M++YGP+ G VS + SMKEMVKTMHA+GIEVL+EVVF++
Sbjct: 402  EPVFTSDEKKGPYFPCHFFSLMHIYGPSGGPVSTMASMKEMVKTMHANGIEVLVEVVFSN 461

Query: 1806 TVEGGALQGIDDSSYYM-NEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFI 1982
            T E GALQGIDDSSYY+ N V +L  ++ALNCNYPIVQ +I+DSLR+WV EF +DGF FI
Sbjct: 462  TAEIGALQGIDDSSYYLANGVGDLKIQSALNCNYPIVQNLILDSLRYWVTEFHIDGFSFI 521

Query: 1983 NSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEM 2162
            N+S LLRGFHGEYLSRPPLVE IAFDP+LSKTKIIAD WDPHD   K+I FPHWMRWAEM
Sbjct: 522  NASHLLRGFHGEYLSRPPLVEAIAFDPVLSKTKIIADCWDPHDTVAKEIHFPHWMRWAEM 581

Query: 2163 NSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFS 2342
            N++FCNDVR F +G+ LLSDLATRLCGSGD+FS  RGPAFSFN+I +N G  LVDLVSFS
Sbjct: 582  NAKFCNDVRNFFRGENLLSDLATRLCGSGDMFSGGRGPAFSFNYIVRNFGFSLVDLVSFS 641

Query: 2343 NTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGS 2522
            +     ELSWNCGEEGPTN  +VLERRLKQIRN+LF+L++SLGVP+LNMGDEC  S GG 
Sbjct: 642  SD---DELSWNCGEEGPTNNTSVLERRLKQIRNFLFILFVSLGVPVLNMGDECAHSSGGF 698

Query: 2523 LAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSE 2702
             AY D KP  W+AL TGFG Q +QFI F++SLR RR DLLQ+R+FLK ENI+W+GSD + 
Sbjct: 699  PAYDDIKPMTWSALTTGFGKQISQFIFFMSSLRRRRSDLLQRRSFLKEENIEWYGSDGAP 758

Query: 2703 LKWDDPSSKFLAMTLKADIA----ESERNPMWGDLFIAINAADCSECVILPPPPEGMAWL 2870
             +W+DPS KFLAMTLK+++A     S  + + GD+FIA+N AD  E  +LP PPEGM+W 
Sbjct: 759  PRWEDPSCKFLAMTLKSEVAVLSESSVSSDISGDIFIALNVADEPESTVLPLPPEGMSWY 818

Query: 2871 RLVDTALPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFETCSPS 3008
            RLVDT+LPFPGFFS++GE V E  AGL  Y+M S+SC LFE C+P+
Sbjct: 819  RLVDTSLPFPGFFSSSGEIVPEMKAGLSTYKMKSHSCALFEACNPT 864


>ref|XP_004493650.1| PREDICTED: isoamylase 2, chloroplastic-like [Cicer arietinum]
          Length = 858

 Score = 1001 bits (2589), Expect = 0.0
 Identities = 515/815 (63%), Positives = 615/815 (75%), Gaps = 10/815 (1%)
 Frame = +3

Query: 579  NLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIG-GHVKVFVGKKNIKYAV 755
            NL  KL ATS++SV +TEQ+ +T T+  +++  LSYLFRTEIG G VKV+V KK + Y V
Sbjct: 57   NLTSKLCATSRLSVEETEQKFSTFTQSEDIKTALSYLFRTEIGEGLVKVYVKKKKVTYFV 116

Query: 756  YIEVSSLLRLNSDSE-LILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSSGIF 932
            YIEVSSL  + ++ E L+L WG++R DS           ++    G   +PF Q S G F
Sbjct: 117  YIEVSSLELVRAEGETLVLCWGVYRDDSL----------SVNVGKGMNVSPFVQNSLGKF 166

Query: 933  TTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGLSFRV 1112
            + +L F+ +Q P YLSFLL+ S        EIR+H K NFCVPVGF  G P+ LGLSF  
Sbjct: 167  SVELEFDVEQVPFYLSFLLRYS------GLEIRTHMKRNFCVPVGFYKGCPSLLGLSFSP 220

Query: 1113 DGSSNFAIFSRNAEGVILCLYDDAK--ADKPALEIDLDPYVNRSGDIWHASIEILPNFVS 1286
            D S NFA+FSR AE V+LCLYDD     +KPALE+DLDPYVNRSGDIWH S E + NFV 
Sbjct: 221  DESVNFAVFSRRAESVVLCLYDDDNDSVEKPALELDLDPYVNRSGDIWHISFESVGNFVR 280

Query: 1287 YGYRFRG-EGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAFDWSGDVR 1463
            YGYR  G   D    E V+LDPYA+I+GN+  +  GS   VK+LG + ++PAFDW  D  
Sbjct: 281  YGYRLGGAHRDNSYAECVVLDPYARIVGNSFPNGIGS---VKNLGFLKKDPAFDWGDDYH 337

Query: 1464 PNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVLLEPIFAF 1643
             NL MEKL+VYRLNV  FT+ +SSQLP ++AGTFSGL +K+ HFKDLG+NAVLLEP+F F
Sbjct: 338  LNLDMEKLLVYRLNVKRFTEHESSQLPGDLAGTFSGLAKKVQHFKDLGMNAVLLEPVFTF 397

Query: 1644 DEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFTHTVEGGA 1823
            DE+ GPY PC+FFSPMNLYG +   VS INSMK+MVKTMHA+GIEVL+EVVF++T E GA
Sbjct: 398  DEEKGPYFPCNFFSPMNLYGKSGDPVSTINSMKDMVKTMHANGIEVLMEVVFSNTAETGA 457

Query: 1824 LQGIDDSSYYM-NEVANLGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCFINSSSLL 2000
            L GIDD SYY  N V  L  ++ALNCNYPI+Q +I+DSLR+WV EF +DGF F+N+S LL
Sbjct: 458  LHGIDDLSYYYANGVGGLKVQSALNCNYPIMQNLILDSLRYWVTEFHIDGFSFVNASHLL 517

Query: 2001 RGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAEMNSRFCN 2180
            RGFHGEYLSRPPLVE IAFDP+L KTKIIAD WD + M  K+IRFPHWMRWAE+N+ FCN
Sbjct: 518  RGFHGEYLSRPPLVEAIAFDPILWKTKIIADCWDLNAMEAKEIRFPHWMRWAEINTNFCN 577

Query: 2181 DVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSFSNTKLAS 2360
            DVR FL+G+ LLS+LATRLCGSGD++S  RGPAFSFN+IA+N GL LVDLVSFS+  L  
Sbjct: 578  DVRNFLRGENLLSNLATRLCGSGDMYSCGRGPAFSFNYIARNFGLSLVDLVSFSSADLDV 637

Query: 2361 ELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGGSLAYSDR 2540
            ELSWNCGEEGPTN  AVLERRLKQIRN+LF+L++SLGVP+LNMGDECG S GGS AY D 
Sbjct: 638  ELSWNCGEEGPTNNTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGYSSGGSPAYGDT 697

Query: 2541 KPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQSELKWDDP 2720
            KPF+W ALKTGFG QTTQFISFLTSLR RR DLLQ ++FLK ENI+W G D +  +W+DP
Sbjct: 698  KPFNWAALKTGFGKQTTQFISFLTSLRKRRSDLLQSKSFLKEENIEWRGVDNAPPRWEDP 757

Query: 2721 SSKFLAMTLKADIAE----SERNPMWGDLFIAINAADCSECVILPPPPEGMAWLRLVDTA 2888
            S KFLAM LKA+  E    S  + + GDLFI  NA D  E V+LP  PEG++W RLVDTA
Sbjct: 758  SCKFLAMNLKAEKGELQESSVSSDILGDLFIVFNADDHPETVVLPLLPEGVSWYRLVDTA 817

Query: 2889 LPFPGFFSTNGEPVLEQTAGLYAYEMSSYSCTLFE 2993
            LPFPGFF TNG+ V EQ +GL  YEM SYSCTLFE
Sbjct: 818  LPFPGFFLTNGDFVPEQISGLCTYEMKSYSCTLFE 852


>ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] gi|42571321|ref|NP_973751.1|
            Isoamylase 2 [Arabidopsis thaliana]
            gi|251764780|sp|Q8L735.2|ISOA2_ARATH RecName:
            Full=Isoamylase 2, chloroplastic; Short=AtISA2; AltName:
            Full=Protein DEBRANCHING ENZYME 1; Flags: Precursor
            gi|3850573|gb|AAC72113.1| Similar to gi|1652733 glycogen
            operon protein GlgX from Synechocystis sp. genome
            gb|D90908. ESTs gb|H36690, gb|AA712462, gb|AA651230 and
            gb|N95932 come from this gene [Arabidopsis thaliana]
            gi|332189436|gb|AEE27557.1| Isoamylase 2 [Arabidopsis
            thaliana] gi|332189437|gb|AEE27558.1| Isoamylase 2
            [Arabidopsis thaliana]
          Length = 882

 Score =  978 bits (2527), Expect = 0.0
 Identities = 498/888 (56%), Positives = 631/888 (71%), Gaps = 20/888 (2%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIG---NKAICGLEKIHMGRKTEVAQKFT 563
            MA   PSV I   C+N G T + K  FP+  +     NK   G E +   RK  +    T
Sbjct: 1    MAAWSPSVGIGSCCLNNGITRTWK--FPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVT 58

Query: 564  KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 743
             + L++  F  +  S+ S+   E   +   E  +L++  SY FRT+ G  VKV V KK  
Sbjct: 59   -SALQSYQFSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKRE 117

Query: 744  KYAVYIEVSSLLRLNSD-SELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKS 920
            KY++ + VSSL     D S L++ WG++RSDSSC +P+DF+NS+  ++T T ET F + S
Sbjct: 118  KYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSS 177

Query: 921  SGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGL 1100
                   L F+ K++P YLSF LK     D +  E+ +HR T+FC+PVGF +G P PLGL
Sbjct: 178  LSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGL 237

Query: 1101 SFRVDGSS-NFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPN 1277
            S   D  S NF+ FSR++  V+LCLYDD+  DKPALE+DLDPYVNR+GD+WHAS++   +
Sbjct: 238  SSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWD 297

Query: 1278 FVSYGYRFRG-----EGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAF 1442
            FV YGYR +      E  + + E ++LDPYA ++G +VS         K+LG +S+ P+F
Sbjct: 298  FVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ--------KYLGSLSKSPSF 349

Query: 1443 DWSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVL 1622
            DW  DV PN+P+EKL+VYRLNV  FT+ +SS+LP NVAGTFSG+ EK+ H K LG NAVL
Sbjct: 350  DWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVL 409

Query: 1623 LEPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFT 1802
            LEPIF+F EQ GPY P HFFSPM++YGP+N   SA+NSMK MVK +H+ GIEVLLEVVFT
Sbjct: 410  LEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFT 469

Query: 1803 HTVEGGALQGIDDSSYYMNEVAN-LGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCF 1979
            HT + GAL+GIDDSSYY    AN L +++ LNCNYP+VQQ++++SLR+WV EF VDGFCF
Sbjct: 470  HTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCF 529

Query: 1980 INSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAE 2159
            IN+SSLLRG HGE LSRPPLVE IAFDPLL++TK+IAD WDP +M PK++RFPHW RWAE
Sbjct: 530  INASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAE 589

Query: 2160 MNSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSF 2339
            +N+R+C +VR FL+G  +LSDLATR+CGSGD+F+D RGPAFSFN+I++NSGL LVD+VSF
Sbjct: 590  LNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSF 649

Query: 2340 SNTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGG 2519
            S  +LASELSWNCGEEG TNK AVL+RRLKQIRN+LF+ YISLGVP+LNMGDECG S  G
Sbjct: 650  SGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRG 709

Query: 2520 SLAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQS 2699
            S     RKPFDWN L + FG Q TQFISF+TS+R RR D+ Q+R+FLK ENI W+ +DQ+
Sbjct: 710  SPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQT 769

Query: 2700 ELKWDDPSSKFLAMTLKADIAESE-------RNPMWGDLFIAINAADCSECVILPPPPEG 2858
              KW+DP+SKFLA+ +K++  E E         P   DLFI  NA+D  E V+LP  P+G
Sbjct: 770  TPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDG 829

Query: 2859 MAWLRLVDTALPFPGFFSTNGEPVL--EQTAGLYAYEMSSYSCTLFET 2996
              W RLVDTALPFPGFFS  GE V+  E    L  YEM  YSCTLFET
Sbjct: 830  SKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFET 877


>gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]
          Length = 882

 Score =  977 bits (2526), Expect = 0.0
 Identities = 498/888 (56%), Positives = 631/888 (71%), Gaps = 20/888 (2%)
 Frame = +3

Query: 393  MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIG---NKAICGLEKIHMGRKTEVAQKFT 563
            MA   PSV I   C+N G T + K  FP+  +     NK   G E +   RK  +    T
Sbjct: 1    MAAWSPSVGIGSCCLNNGITRTWK--FPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVT 58

Query: 564  KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 743
             + L++  F  +  S+ S+   E   +   E  +L++  SY FRT+ G  VKV V KK  
Sbjct: 59   -SALQSYQFSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKRE 117

Query: 744  KYAVYIEVSSLLRLNSD-SELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKS 920
            KY++ + VSSL     D S L++ WG++RSDSSC +P+DF+NS+  ++T T ET F + S
Sbjct: 118  KYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSS 177

Query: 921  SGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPLGL 1100
                   L F+ K++P YLSF LK     D +  E+ +HR T+FC+PVGF +G P PLGL
Sbjct: 178  LSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGREMLTHRDTDFCIPVGFTAGHPLPLGL 237

Query: 1101 SFRVDGSS-NFAIFSRNAEGVILCLYDDAKADKPALEIDLDPYVNRSGDIWHASIEILPN 1277
            S   D  S NF+ FSR++  V+LCLYDD+  DKPALE+DLDPYVNR+GD+WHAS++   +
Sbjct: 238  SSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWD 297

Query: 1278 FVSYGYRFRG-----EGDEFDTERVILDPYAKIIGNAVSDHHGSGFPVKHLGQVSREPAF 1442
            FV YGYR +      E  + + E ++LDPYA ++G +VS         K+LG +S+ P+F
Sbjct: 298  FVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ--------KYLGSLSKSPSF 349

Query: 1443 DWSGDVRPNLPMEKLVVYRLNVMHFTKDKSSQLPVNVAGTFSGLTEKLHHFKDLGVNAVL 1622
            DW  DV PN+P+EKL+VYRLNV  FT+ +SS+LP NVAGTFSG+ EK+ H K LG NAVL
Sbjct: 350  DWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVL 409

Query: 1623 LEPIFAFDEQTGPYLPCHFFSPMNLYGPTNGCVSAINSMKEMVKTMHASGIEVLLEVVFT 1802
            LEPIF+F EQ GPY P HFFSPM++YGP+N   SA+NSMK MVK +H+ GIEVLLEVVFT
Sbjct: 410  LEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFT 469

Query: 1803 HTVEGGALQGIDDSSYYMNEVAN-LGTRNALNCNYPIVQQMIVDSLRHWVIEFRVDGFCF 1979
            HT + GAL+GIDDSSYY    AN L +++ LNCNYP+VQQ++++SLR+WV EF VDGFCF
Sbjct: 470  HTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCF 529

Query: 1980 INSSSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADNWDPHDMAPKDIRFPHWMRWAE 2159
            IN+SSLLRG HGE LSRPPLVE IAFDPLL++TK+IAD WDP +M PK++RFPHW RWAE
Sbjct: 530  INASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAE 589

Query: 2160 MNSRFCNDVRKFLKGDALLSDLATRLCGSGDIFSDRRGPAFSFNFIAKNSGLPLVDLVSF 2339
            +N+R+C +VR FL+G  +LSDLATR+CGSGD+F+D RGPAFSFN+I++NSGL LVD+VSF
Sbjct: 590  LNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSF 649

Query: 2340 SNTKLASELSWNCGEEGPTNKKAVLERRLKQIRNYLFVLYISLGVPILNMGDECGQSYGG 2519
            S  +LASELSWNCGEEG TNK AVL+RRLKQIRN+LF+ YISLGVP+LNMGDECG S  G
Sbjct: 650  SGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRG 709

Query: 2520 SLAYSDRKPFDWNALKTGFGIQTTQFISFLTSLRIRRGDLLQKRNFLKVENIDWHGSDQS 2699
            S     RKPFDWN L + FG Q TQFISF+TS+R RR D+ Q+R+FLK ENI W+ +DQ+
Sbjct: 710  SPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQT 769

Query: 2700 ELKWDDPSSKFLAMTLKADIAESE-------RNPMWGDLFIAINAADCSECVILPPPPEG 2858
              KW+DP+SKFLA+ +K++  E E         P   DLFI  NA+D  E V+LP  P+G
Sbjct: 770  TPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDG 829

Query: 2859 MAWLRLVDTALPFPGFFSTNGEPVL--EQTAGLYAYEMSSYSCTLFET 2996
              W RLVDTALPFPGFFS  GE V+  E    L  YEM  YSCTLFET
Sbjct: 830  SKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFET 877