BLASTX nr result
ID: Paeonia24_contig00012745
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Paeonia24_contig00012745
(392 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006440440.1| hypothetical protein CICLE_v10019622mg [Citr... 90 8e-25
ref|XP_006477310.1| PREDICTED: MATE efflux family protein DTX1-l... 87 9e-24
ref|XP_006368730.1| MATE efflux family protein [Populus trichoca... 80 9e-23
ref|XP_002304444.2| MATE efflux family protein [Populus trichoca... 82 3e-22
ref|XP_002509634.1| multidrug resistance pump, putative [Ricinus... 75 4e-21
ref|XP_002274870.2| PREDICTED: MATE efflux family protein DTX1-l... 92 7e-17
emb|CAN82072.1| hypothetical protein VITISV_016012 [Vitis vinifera] 90 3e-16
ref|NP_194010.1| mate efflux domain-containing protein [Arabidop... 68 9e-16
ref|XP_006363917.1| PREDICTED: MATE efflux family protein DTX1-l... 81 1e-13
ref|XP_004245689.1| PREDICTED: MATE efflux family protein DTX1-l... 81 2e-13
ref|XP_004242013.1| PREDICTED: MATE efflux family protein DTX1-l... 81 2e-13
ref|XP_006363710.1| PREDICTED: MATE efflux family protein DTX1-l... 79 9e-13
ref|XP_004298710.1| PREDICTED: MATE efflux family protein 9-like... 77 2e-12
ref|XP_007040017.1| Multidrug and toxin extrusion protein 1 [The... 77 2e-12
ref|XP_006413620.1| hypothetical protein EUTSA_v10025023mg [Eutr... 75 9e-12
ref|XP_007209922.1| hypothetical protein PRUPE_ppa004487mg [Prun... 74 2e-11
ref|XP_006283611.1| hypothetical protein CARUB_v10004667mg [Caps... 74 2e-11
ref|XP_002867749.1| mate efflux family protein [Arabidopsis lyra... 73 4e-11
ref|XP_004980967.1| PREDICTED: MATE efflux family protein 9-like... 72 6e-11
ref|XP_003550892.2| PREDICTED: MATE efflux family protein 5-like... 71 1e-10
>ref|XP_006440440.1| hypothetical protein CICLE_v10019622mg [Citrus clementina]
gi|557542702|gb|ESR53680.1| hypothetical protein
CICLE_v10019622mg [Citrus clementina]
Length = 540
Score = 90.1 bits (222), Expect(2) = 8e-25
Identities = 46/82 (56%), Positives = 58/82 (70%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALP+ VVLAFK+ LGL GLLIGF +GM ARI+W +EA KA+ILAC
Sbjct: 428 FYLLALPVCVVLAFKAALGLAGLLIGFFIGMVACLALLLTFIARINWDDEAGKAQILACD 487
Query: 258 IQEDVKEDENHKTLELVENVDV 323
+QED KEDEN ++ + E+V V
Sbjct: 488 VQEDAKEDENVRSAVVDESVSV 509
Score = 49.3 bits (116), Expect(2) = 8e-25
Identities = 20/24 (83%), Positives = 23/24 (95%)
Frame = +2
Query: 2 PVAVCGGIVRGTARPWLAMYANLG 73
P+ VCGGIVRGTARPWLAM+AN+G
Sbjct: 403 PLTVCGGIVRGTARPWLAMHANVG 426
>ref|XP_006477310.1| PREDICTED: MATE efflux family protein DTX1-like [Citrus sinensis]
Length = 540
Score = 86.7 bits (213), Expect(2) = 9e-24
Identities = 46/82 (56%), Positives = 58/82 (70%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALP+ VVLAFK+ LGL GLLIG L+G+ ARI+W +EA KA+ILAC
Sbjct: 428 FYLLALPVCVVLAFKAALGLAGLLIGLLIGIIACLALLLMFIARINWDDEAGKAQILACD 487
Query: 258 IQEDVKEDENHKTLELVENVDV 323
+QEDVKEDEN ++ E+V V
Sbjct: 488 VQEDVKEDENVRSAVDDESVPV 509
Score = 49.3 bits (116), Expect(2) = 9e-24
Identities = 20/24 (83%), Positives = 23/24 (95%)
Frame = +2
Query: 2 PVAVCGGIVRGTARPWLAMYANLG 73
P+ VCGGIVRGTARPWLAM+AN+G
Sbjct: 403 PLTVCGGIVRGTARPWLAMHANVG 426
>ref|XP_006368730.1| MATE efflux family protein [Populus trichocarpa]
gi|550346818|gb|ERP65299.1| MATE efflux family protein
[Populus trichocarpa]
Length = 475
Score = 80.1 bits (196), Expect(2) = 9e-23
Identities = 42/83 (50%), Positives = 55/83 (66%), Gaps = 2/83 (2%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILA-C 254
FY +ALP+ V+LAFK+ LGL GLL+GFL+G+ RIDW EA+KA+ LA C
Sbjct: 392 FYFLALPMAVLLAFKAALGLGGLLVGFLIGLVACLILLVVFVVRIDWEVEAEKAQKLASC 451
Query: 255 SIQE-DVKEDENHKTLELVENVD 320
+QE DVKE NH+T E V+ +
Sbjct: 452 DVQEVDVKERVNHRTTETVDGAE 474
Score = 52.4 bits (124), Expect(2) = 9e-23
Identities = 22/24 (91%), Positives = 23/24 (95%)
Frame = +2
Query: 2 PVAVCGGIVRGTARPWLAMYANLG 73
P+AVCGGIVRGTARPWL MYANLG
Sbjct: 367 PLAVCGGIVRGTARPWLGMYANLG 390
>ref|XP_002304444.2| MATE efflux family protein [Populus trichocarpa]
gi|550342988|gb|EEE79423.2| MATE efflux family protein
[Populus trichocarpa]
Length = 509
Score = 81.6 bits (200), Expect(2) = 3e-22
Identities = 43/85 (50%), Positives = 57/85 (67%), Gaps = 3/85 (3%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILA-C 254
FY +A+P+ ++LAFK+ LGL GLLIGFL+G+A RIDW EADKA+ LA C
Sbjct: 425 FYFLAVPIAILLAFKAALGLGGLLIGFLIGLAACLILLVGFVVRIDWQVEADKAQKLASC 484
Query: 255 SIQEDV--KEDENHKTLELVENVDV 323
+QEDV KE NH+T+E + +V
Sbjct: 485 DVQEDVRIKECVNHRTIETEDGAEV 509
Score = 48.9 bits (115), Expect(2) = 3e-22
Identities = 20/24 (83%), Positives = 22/24 (91%)
Frame = +2
Query: 2 PVAVCGGIVRGTARPWLAMYANLG 73
P+AVCGGI RGTARPWL+ YANLG
Sbjct: 400 PLAVCGGIARGTARPWLSTYANLG 423
>ref|XP_002509634.1| multidrug resistance pump, putative [Ricinus communis]
gi|223549533|gb|EEF51021.1| multidrug resistance pump,
putative [Ricinus communis]
Length = 506
Score = 74.7 bits (182), Expect(2) = 4e-21
Identities = 39/82 (47%), Positives = 55/82 (67%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALP+ V+LAFK+RLGL GLL+GFLVG++ RIDW EADKA+ LA
Sbjct: 426 FYLLALPVAVLLAFKARLGLGGLLLGFLVGLSACLALLVVFIMRIDWDVEADKAQKLASD 485
Query: 258 IQEDVKEDENHKTLELVENVDV 323
+QE+++ EN + ++ +V
Sbjct: 486 VQENIQR-ENQRPNRTPDDAEV 506
Score = 52.4 bits (124), Expect(2) = 4e-21
Identities = 21/24 (87%), Positives = 24/24 (100%)
Frame = +2
Query: 2 PVAVCGGIVRGTARPWLAMYANLG 73
P+AVCGG+VRGTARPWLAMY+NLG
Sbjct: 401 PLAVCGGVVRGTARPWLAMYSNLG 424
>ref|XP_002274870.2| PREDICTED: MATE efflux family protein DTX1-like [Vitis vinifera]
Length = 484
Score = 92.0 bits (227), Expect = 7e-17
Identities = 48/79 (60%), Positives = 54/79 (68%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYLVALPLGVV AFK+ LGL GLLIG LVG+ RIDW E A KA+ILAC
Sbjct: 405 FYLVALPLGVVFAFKAGLGLGGLLIGLLVGVVVCLALLLVFIFRIDWAEAAGKAQILACR 464
Query: 258 IQEDVKEDENHKTLELVEN 314
QE KEDENH+ L+ +EN
Sbjct: 465 EQEIAKEDENHRILKTLEN 483
>emb|CAN82072.1| hypothetical protein VITISV_016012 [Vitis vinifera]
Length = 484
Score = 90.1 bits (222), Expect = 3e-16
Identities = 47/79 (59%), Positives = 53/79 (67%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYLVALPLG V AFK+ LGL GLLIG LVG+ RIDW E A KA+ILAC
Sbjct: 405 FYLVALPLGXVFAFKAGLGLGGLLIGLLVGVVVCLALLLVFIFRIDWAEAAGKAQILACR 464
Query: 258 IQEDVKEDENHKTLELVEN 314
QE KEDENH+ L+ +EN
Sbjct: 465 EQEIAKEDENHRILKTLEN 483
>ref|NP_194010.1| mate efflux domain-containing protein [Arabidopsis thaliana]
gi|2827556|emb|CAA16564.1| predicted protein
[Arabidopsis thaliana] gi|7269126|emb|CAB79234.1|
predicted protein [Arabidopsis thaliana]
gi|27754339|gb|AAO22621.1| putative integral membrane
protein [Arabidopsis thaliana]
gi|332659258|gb|AEE84658.1| mate efflux
domain-containing protein [Arabidopsis thaliana]
Length = 491
Score = 68.2 bits (165), Expect(2) = 9e-16
Identities = 32/64 (50%), Positives = 43/64 (67%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALPLG LAFK++ GL G LIG VG++ ARIDW +EA KA+IL C+
Sbjct: 416 FYLLALPLGATLAFKAKQGLQGFLIGLFVGISLCLSILLIFIARIDWEKEAGKAQILTCN 475
Query: 258 IQED 269
+++
Sbjct: 476 TEDE 479
Score = 40.8 bits (94), Expect(2) = 9e-16
Identities = 20/34 (58%), Positives = 22/34 (64%)
Frame = +2
Query: 2 PVAVCGGIVRGTARPWLAMYANLGVVLLSGFALG 103
P+ VCG IVRGTA+P L MYANL L LG
Sbjct: 391 PLMVCGEIVRGTAKPSLGMYANLSGFYLLALPLG 424
>ref|XP_006363917.1| PREDICTED: MATE efflux family protein DTX1-like [Solanum tuberosum]
Length = 486
Score = 81.3 bits (199), Expect = 1e-13
Identities = 42/78 (53%), Positives = 55/78 (70%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALP GV+LAFK +LGL GLLIGF+VG+A ARIDWV EA+KA LAC+
Sbjct: 405 FYLLALPSGVILAFKVQLGLAGLLIGFMVGVAACLALLLVLIARIDWVGEANKAHTLACN 464
Query: 258 IQEDVKEDENHKTLELVE 311
+E +D++ KT +V+
Sbjct: 465 QEEGGGDDDDRKTSWVVK 482
>ref|XP_004245689.1| PREDICTED: MATE efflux family protein DTX1-like [Solanum
lycopersicum]
Length = 480
Score = 80.9 bits (198), Expect = 2e-13
Identities = 39/69 (56%), Positives = 51/69 (73%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALPLGVVLAFK +GL GLL GF+VG+A ARIDWV+EA KA++L+C+
Sbjct: 410 FYLLALPLGVVLAFKIHVGLAGLLTGFVVGVACCLALLLVFIARIDWVQEAKKAQLLSCN 469
Query: 258 IQEDVKEDE 284
++E +DE
Sbjct: 470 LEEIANDDE 478
>ref|XP_004242013.1| PREDICTED: MATE efflux family protein DTX1-like [Solanum
lycopersicum]
Length = 487
Score = 80.9 bits (198), Expect = 2e-13
Identities = 42/79 (53%), Positives = 57/79 (72%), Gaps = 1/79 (1%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALPLGV+LAFK +LGL GLLIGF+VG+ ARIDWV EA+KA +LAC+
Sbjct: 405 FYLLALPLGVILAFKVQLGLAGLLIGFMVGVVACLALLLGLIARIDWVGEANKAHMLACN 464
Query: 258 IQEDVK-EDENHKTLELVE 311
+E + +D++ KT +V+
Sbjct: 465 QEEGGRDDDDDRKTSSVVK 483
>ref|XP_006363710.1| PREDICTED: MATE efflux family protein DTX1-like [Solanum tuberosum]
Length = 480
Score = 78.6 bits (192), Expect = 9e-13
Identities = 39/71 (54%), Positives = 50/71 (70%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALPLGVVLAFK LGL GLL GF+VG+A ARIDW +EA KA++L+C+
Sbjct: 410 FYLLALPLGVVLAFKIHLGLAGLLTGFVVGVACCLALLLVFIARIDWSQEAKKAQLLSCN 469
Query: 258 IQEDVKEDENH 290
++E DE +
Sbjct: 470 LEEIANVDERN 480
>ref|XP_004298710.1| PREDICTED: MATE efflux family protein 9-like [Fragaria vesca subsp.
vesca]
Length = 506
Score = 77.4 bits (189), Expect = 2e-12
Identities = 45/80 (56%), Positives = 54/80 (67%), Gaps = 2/80 (2%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKA-RILAC 254
FYL+ALPLGVVLAFK +LGL GLLIGFL+GM RI+W EEA KA R+ +
Sbjct: 424 FYLLALPLGVVLAFKVKLGLSGLLIGFLIGMVACLILLMMFVLRINWDEEACKAQRLASV 483
Query: 255 SIQEDV-KEDENHKTLELVE 311
S Q V +DENHK++ VE
Sbjct: 484 SCQAPVLNKDENHKSVGSVE 503
Score = 58.2 bits (139), Expect = 1e-06
Identities = 31/66 (46%), Positives = 39/66 (59%)
Frame = +2
Query: 2 PVAVCGGIVRGTARPWLAMYANLGVVLLSGFALGCGFSIQI*AWA*WTVDWVLGWDGCVL 181
P+AVCGGIVRGTARPWLAMYANLG L LG + ++ LG G ++
Sbjct: 399 PLAVCGGIVRGTARPWLAMYANLGGFYLLALPLGVVLAFKV----------KLGLSGLLI 448
Query: 182 AFGLGV 199
F +G+
Sbjct: 449 GFLIGM 454
>ref|XP_007040017.1| Multidrug and toxin extrusion protein 1 [Theobroma cacao]
gi|508777262|gb|EOY24518.1| Multidrug and toxin
extrusion protein 1 [Theobroma cacao]
Length = 504
Score = 77.0 bits (188), Expect = 2e-12
Identities = 43/82 (52%), Positives = 54/82 (65%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALP+ VVLAFK+ LGL+GLL+GFLVGM RI W EEA KA+ILA +
Sbjct: 425 FYLLALPMAVVLAFKTALGLNGLLLGFLVGMVTCLALLLVMVVRIKWHEEAGKAQILASN 484
Query: 258 IQEDVKEDENHKTLELVENVDV 323
+ V ED N KT++ V +V
Sbjct: 485 VA--VVEDGNLKTVDTVNPTEV 504
>ref|XP_006413620.1| hypothetical protein EUTSA_v10025023mg [Eutrema salsugineum]
gi|557114790|gb|ESQ55073.1| hypothetical protein
EUTSA_v10025023mg [Eutrema salsugineum]
Length = 491
Score = 75.1 bits (183), Expect = 9e-12
Identities = 36/64 (56%), Positives = 46/64 (71%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALPLG LAFK++LGL+G LIGFLVG + AR+DW +EA KA+IL CS
Sbjct: 415 FYLIALPLGASLAFKAKLGLEGFLIGFLVGASVCFSVLLIFIARLDWEKEAGKAQILTCS 474
Query: 258 IQED 269
+E+
Sbjct: 475 REEE 478
>ref|XP_007209922.1| hypothetical protein PRUPE_ppa004487mg [Prunus persica]
gi|462405657|gb|EMJ11121.1| hypothetical protein
PRUPE_ppa004487mg [Prunus persica]
Length = 507
Score = 74.3 bits (181), Expect = 2e-11
Identities = 43/78 (55%), Positives = 50/78 (64%), Gaps = 3/78 (3%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARIL--- 248
FYL+ALPLGVVLAFK LGL GLLIGFL+GM RI+W EEA KA+ L
Sbjct: 425 FYLLALPLGVVLAFKVALGLSGLLIGFLIGMVACLILVLMFVVRINWDEEACKAQTLASV 484
Query: 249 ACSIQEDVKEDENHKTLE 302
AC E + +D NHK+ E
Sbjct: 485 ACQAPE-LNKDGNHKSTE 501
Score = 58.9 bits (141), Expect = 7e-07
Identities = 31/66 (46%), Positives = 39/66 (59%)
Frame = +2
Query: 2 PVAVCGGIVRGTARPWLAMYANLGVVLLSGFALGCGFSIQI*AWA*WTVDWVLGWDGCVL 181
P+AVCGGIVRGTARPWLAMYANLG L LG + ++ LG G ++
Sbjct: 400 PLAVCGGIVRGTARPWLAMYANLGGFYLLALPLGVVLAFKV----------ALGLSGLLI 449
Query: 182 AFGLGV 199
F +G+
Sbjct: 450 GFLIGM 455
>ref|XP_006283611.1| hypothetical protein CARUB_v10004667mg [Capsella rubella]
gi|482552316|gb|EOA16509.1| hypothetical protein
CARUB_v10004667mg [Capsella rubella]
Length = 492
Score = 73.9 bits (180), Expect = 2e-11
Identities = 35/64 (54%), Positives = 46/64 (71%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILACS 257
FYL+ALPLG LAFK++ GL+G LIGFLVG++ ARIDW +EA KA+IL C+
Sbjct: 416 FYLLALPLGATLAFKAKRGLEGFLIGFLVGISVCFSILLIFIARIDWEKEAGKAQILTCN 475
Query: 258 IQED 269
+E+
Sbjct: 476 TEEE 479
>ref|XP_002867749.1| mate efflux family protein [Arabidopsis lyrata subsp. lyrata]
gi|297313585|gb|EFH44008.1| mate efflux family protein
[Arabidopsis lyrata subsp. lyrata]
Length = 487
Score = 73.2 bits (178), Expect = 4e-11
Identities = 36/67 (53%), Positives = 46/67 (68%)
Frame = +3
Query: 69 LVWFYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARIL 248
L FYL+ALPLG LAFK++ GL+G LIGFLVG++ ARIDW +EA KA+IL
Sbjct: 415 LTGFYLLALPLGATLAFKAKQGLEGFLIGFLVGISVCLSILLIFIARIDWEKEAGKAQIL 474
Query: 249 ACSIQED 269
C+ E+
Sbjct: 475 TCNTVEE 481
>ref|XP_004980967.1| PREDICTED: MATE efflux family protein 9-like [Setaria italica]
Length = 546
Score = 72.4 bits (176), Expect = 6e-11
Identities = 37/74 (50%), Positives = 48/74 (64%)
Frame = +3
Query: 66 ILVWFYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARI 245
++ FYLVALP+GV L FK+RLGL+GLL GFLVG A +DWV EADKA+
Sbjct: 462 VVAGFYLVALPVGVALGFKARLGLEGLLAGFLVGAAASLAVLVTVIVCMDWVAEADKAQK 521
Query: 246 LACSIQEDVKEDEN 287
A + +D +ED +
Sbjct: 522 RAGADDQDTQEDNS 535
>ref|XP_003550892.2| PREDICTED: MATE efflux family protein 5-like [Glycine max]
Length = 507
Score = 71.2 bits (173), Expect = 1e-10
Identities = 36/72 (50%), Positives = 47/72 (65%), Gaps = 3/72 (4%)
Frame = +3
Query: 78 FYLVALPLGVVLAFKSRLGLDGLLIGFLVGMAXXXXXXXXXXARIDWVEEADKARILAC- 254
FY +ALPLGVV AFK RLGL GL+IGFL+G+ RI+WV+EA KA++L C
Sbjct: 430 FYFLALPLGVVFAFKLRLGLAGLIIGFLIGVVACLILLLTFIVRINWVQEATKAQMLVCI 489
Query: 255 --SIQEDVKEDE 284
+QE V ++
Sbjct: 490 PAQVQEQVPRNQ 501
Score = 56.6 bits (135), Expect = 3e-06
Identities = 30/66 (45%), Positives = 37/66 (56%)
Frame = +2
Query: 2 PVAVCGGIVRGTARPWLAMYANLGVVLLSGFALGCGFSIQI*AWA*WTVDWVLGWDGCVL 181
PV VCGGIVRGTARPWL MYAN+G LG F+ ++ LG G ++
Sbjct: 405 PVTVCGGIVRGTARPWLGMYANIGGFYFLALPLGVVFAFKL----------RLGLAGLII 454
Query: 182 AFGLGV 199
F +GV
Sbjct: 455 GFLIGV 460